Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,404,897 A→G intergenic (‑236/‑684) lrhA ← / → b2290 NADH dehydrogenase transcriptional regulator, LysR family/putative aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,404,8970AG99.3% 1031.7 / ‑4.7 295intergenic (‑236/‑684)lrhA/b2290NADH dehydrogenase transcriptional regulator, LysR family/putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (114/177);  total (116/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 295 total aligned reads displayed.

GCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACC  >  NC_000913/2404806‑2404986
                                                                                           |                                                                                         
 cGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGttt                                                                                        >  2:9531049/1‑94 (MQ=255)
 cGACTCACATTGTGCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCTTATCTATTAAGTTACTCACTCTTTTCTGttt                                                                                        <  2:10523807/94‑1 (MQ=255)
  gACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTa                                                                                       <  2:3609742/94‑1 (MQ=255)
  gACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTa                                                                                       >  2:2849645/1‑94 (MQ=255)
  gACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTa                                                                                       <  1:2882744/94‑1 (MQ=255)
    cTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATg                                                                                     <  1:4310694/94‑1 (MQ=255)
     tCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGa                                                                                    <  1:7255534/94‑1 (MQ=255)
     tCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGa                                                                                    <  1:150047/94‑1 (MQ=255)
     tCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGa                                                                                    <  2:4948703/94‑1 (MQ=255)
     tCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGa                                                                                    >  1:8937199/1‑94 (MQ=255)
     tCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGa                                                                                    <  1:3235977/94‑1 (MQ=255)
      cacaTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGAc                                                                                   <  1:2656984/94‑1 (MQ=255)
       acaTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACa                                                                                  <  1:1821457/94‑1 (MQ=255)
        caTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACAt                                                                                 <  2:8981064/94‑1 (MQ=255)
         aTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATg                                                                                <  1:707298/94‑1 (MQ=255)
          ttGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATgc                                                                               <  2:2047829/94‑1 (MQ=255)
          ttGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATgc                                                                               <  1:8313214/94‑1 (MQ=255)
          ttGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATgc                                                                               <  1:7610986/94‑1 (MQ=255)
            gTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATgcgc                                                                             <  1:9118545/94‑1 (MQ=255)
            gTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATgcgc                                                                             <  2:5603827/94‑1 (MQ=255)
            gTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATgcgc                                                                             >  2:4648513/1‑94 (MQ=255)
             ttCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGcgcg                                                                            <  2:9129227/94‑1 (MQ=255)
               ccGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGGCATGCGCgtg                                                                          >  2:4406364/1‑94 (MQ=255)
                cGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCgtgt                                                                         <  1:3372264/94‑1 (MQ=255)
                 gTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGtt                                                                        >  2:9833012/1‑94 (MQ=255)
                 gTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGtt                                                                        <  2:1077337/94‑1 (MQ=255)
                 gTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGtt                                                                        >  2:7240473/1‑94 (MQ=255)
                 gTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGtt                                                                        >  1:3015699/1‑94 (MQ=255)
                  ttATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGttt                                                                       >  1:6493765/1‑94 (MQ=255)
                  ttATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGttt                                                                       <  2:1723775/94‑1 (MQ=255)
                  ttATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGttt                                                                       <  2:1490332/94‑1 (MQ=255)
                   tataATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTg                                                                      >  1:3580826/1‑94 (MQ=255)
                   tataATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTg                                                                      <  2:6544186/94‑1 (MQ=255)
                   tataATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTg                                                                      >  1:9160455/1‑94 (MQ=255)
                    ataATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGt                                                                     <  1:2234988/94‑1 (MQ=255)
                      aaTGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGtat                                                                   >  2:2215325/1‑94 (MQ=255)
                       aTGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGtata                                                                  >  1:4665915/1‑94 (MQ=255)
                       aTGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGtata                                                                  <  2:6332456/94‑1 (MQ=255)
                       aTGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGtata                                                                  <  1:5334230/94‑1 (MQ=255)
                        tGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaa                                                                 <  1:10592665/94‑1 (MQ=255)
                        tGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaa                                                                 <  1:8688383/94‑1 (MQ=255)
                          ccTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaat                                                               <  2:5312645/94‑1 (MQ=255)
                           cTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatg                                                              <  1:4888980/94‑1 (MQ=255)
                           cTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatg                                                              <  1:189712/94‑1 (MQ=255)
                           cTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatg                                                              >  1:6712567/1‑94 (MQ=255)
                            tGAAGTAGATGACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatgt                                                             <  2:7139398/94‑1 (MQ=255)
                            tGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTTtaaatgt                                                             >  2:5860529/1‑94 (MQ=255)
                            tGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatgt                                                             <  2:3784596/94‑1 (MQ=255)
                            tGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatgt                                                             >  2:758942/1‑94 (MQ=255)
                             gAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatgta                                                            >  2:884399/1‑94 (MQ=255)
                              aaGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatgtaa                                                           <  1:4464868/94‑1 (MQ=255)
                               aGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatgtaaa                                                          <  1:4003015/94‑1 (MQ=255)
                               aGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatgtaaa                                                          >  1:1977523/1‑94 (MQ=255)
                                 tAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAtaaatgtaaatg                                                        >  2:8659180/1‑94 (MQ=255)
                                   gATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAAtgtg                                                      <  1:7901641/94‑1 (MQ=255)
                                   gATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAAtgtg                                                      <  1:9349895/94‑1 (MQ=255)
                                     tCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAg                                                    >  2:6757534/1‑94 (MQ=255)
                                     tCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAg                                                    <  2:2399967/94‑1 (MQ=255)
                                     gcacaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAg                                                    <  1:8557190/93‑1 (MQ=255)
                                      cacaGAATATATCTTCAGGGATCGCATATCTATTAAGTTATTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGt                                                   <  2:8284901/94‑1 (MQ=255)
                                      cacaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGt                                                   >  2:6383551/1‑94 (MQ=255)
                                      cacaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGt                                                   >  1:9560737/1‑94 (MQ=255)
                                       acaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTc                                                  >  1:6198897/1‑94 (MQ=255)
                                       acaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTc                                                  <  2:10167834/94‑1 (MQ=255)
                                         aGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCt                                                >  2:7715192/1‑94 (MQ=255)
                                         aGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCt                                                >  1:567868/1‑94 (MQ=255)
                                         aGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCt                                                >  2:6151483/1‑94 (MQ=255)
                                          gAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTTTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCtt                                               <  2:8506916/94‑1 (MQ=255)
                                          gAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCtt                                               >  1:1020258/1‑94 (MQ=255)
                                           aaTATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTg                                              <  2:7968306/94‑1 (MQ=255)
                                               tatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTcc                                          <  2:579943/94‑1 (MQ=255)
                                                atCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCa                                         <  1:8215803/94‑1 (MQ=255)
                                                  cTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAct                                       <  2:2732902/94‑1 (MQ=255)
                                                   ttCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCActc                                      >  2:7449821/1‑94 (MQ=255)
                                                    tCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCActct                                     <  1:6347275/94‑1 (MQ=255)
                                                      aGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCg                                   <  2:6090558/94‑1 (MQ=255)
                                                        ggATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTg                                 <  1:4267329/94‑1 (MQ=255)
                                                          gacGCATATCTATTAAGTTACTCCCTCCTTTCTGTTTAGGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTgca                               <  2:3110975/92‑1 (MQ=255)
                                                          aTCGCATATTTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGGTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCGCGTgca                               >  1:9394884/1‑94 (MQ=255)
                                                          aTCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTgca                               <  2:2918403/94‑1 (MQ=255)
                                                              cATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCAt                           >  1:9518909/1‑94 (MQ=255)
                                                               atatCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATc                          >  1:2555554/1‑94 (MQ=255)
                                                                 atCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGT‑AATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCt                       <  2:9700899/94‑1 (MQ=255)
                                                                  tCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCt                       >  2:9602011/1‑94 (MQ=255)
                                                                  tCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCt                       <  1:7659801/94‑1 (MQ=255)
                                                                  tCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCt                       <  2:6232923/94‑1 (MQ=255)
                                                                  tCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCt                       >  2:3248775/1‑94 (MQ=255)
                                                                   cTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTg                      <  1:1657993/94‑1 (MQ=255)
                                                                   cTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTg                      >  2:1198799/1‑94 (MQ=255)
                                                                        aaGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCAt                 <  1:7740699/94‑1 (MQ=255)
                                                                        aaGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCAt                 <  1:5033906/94‑1 (MQ=255)
                                                                         aGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATa                >  2:5062430/1‑94 (MQ=255)
                                                                          gTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATAc               <  2:4709544/94‑1 (MQ=255)
                                                                            tACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATAcgc             <  1:1118833/94‑1 (MQ=255)
                                                                              ctcactcTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGa           >  1:5634733/1‑94 (MQ=255)
                                                                                ccctctTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAac         <  1:10562420/92‑1 (MQ=255)
                                                                                  ctctTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAacac       >  1:8046280/1‑94 (MQ=255)
                                                                                   tctTTTCTGTTTATGACATGCGCCTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACg      <  1:5805833/94‑1 (MQ=255)
                                                                                      tttCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTAc   >  1:8480398/1‑94 (MQ=255)
                                                                                      tttCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTAc   <  1:10039783/94‑1 (MQ=255)
                                                                                           |                                                                                         
GCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACC  >  NC_000913/2404806‑2404986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACCAACA  >  NC_000913/2404805‑2404990
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AGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGT                                                                                              >  SRR4302545.6644815/1‑94 (MQ=60)
 GCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTT                                                                                             <  SRR4302545.4635333/94‑1 (MQ=60)
 GCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTT                                                                                             <  SRR4302545.5364536/94‑1 (MQ=60)
   GACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTA                                                                                           <  SRR4302545.2952531/94‑1 (MQ=60)
   GACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTA                                                                                           >  SRR4302545.10436895/1‑94 (MQ=60)
    ACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTAT                                                                                          >  SRR4302545.9914730/1‑94 (MQ=60)
     CTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATG                                                                                         <  SRR4302545.4419251/94‑1 (MQ=60)
      TCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGA                                                                                        <  SRR4302545.153339/94‑1 (MQ=60)
      TCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGA                                                                                        <  SRR4302545.3315439/94‑1 (MQ=60)
      TCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGA                                                                                        <  SRR4302545.5510935/94‑1 (MQ=60)
      TCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGA                                                                                        <  SRR4302545.7458783/94‑1 (MQ=60)
      TCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGA                                                                                        >  SRR4302545.9197073/1‑94 (MQ=60)
       CACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGAC                                                                                       <  SRR4302545.2720992/94‑1 (MQ=60)
        ACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACA                                                                                      >  SRR4302545.6556079/1‑94 (MQ=60)
        ACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACA                                                                                      <  SRR4302545.1865609/94‑1 (MQ=60)
         CATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACAT                                                                                     <  SRR4302545.2870225/94‑1 (MQ=60)
         CATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACAT                                                                                     <  SRR4302545.3296714/94‑1 (MQ=60)
         CATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACAT                                                                                     >  SRR4302545.640260/1‑94 (MQ=60)
         CATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACAT                                                                                     <  SRR4302545.8915648/94‑1 (MQ=60)
          ATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATG                                                                                    <  SRR4302545.723188/94‑1 (MQ=60)
           TTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGC                                                                                   <  SRR4302545.8554109/94‑1 (MQ=60)
           TTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGC                                                                                   <  SRR4302545.7826299/94‑1 (MQ=60)
           TTGTTCCG‑TATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCCTATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCG                                                                                  >  SRR4302545.6604606/1‑94 (MQ=60)
            TGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCG                                                                                  <  SRR4302545.824054/94‑1 (MQ=60)
            TGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCG                                                                                  <  SRR4302545.4411376/94‑1 (MQ=60)
             GTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGC                                                                                 <  SRR4302545.9386119/94‑1 (MQ=60)
              TTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCG                                                                                <  SRR4302545.4270310/94‑1 (MQ=60)
              TTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCG                                                                                <  SRR4302545.9818911/94‑1 (MQ=60)
              TTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCG                                                                                <  SRR4302545.6778092/94‑1 (MQ=60)
              TTCCGTTATAATACCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGGTACTCACTCTTTTCTGTTTATGACATGCGCG                                                                                <  SRR4302545.9254495/94‑1 (MQ=60)
               TCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGT                                                                               >  SRR4302545.2231815/1‑94 (MQ=60)
               TCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGT                                                                               <  SRR4302545.7415599/94‑1 (MQ=60)
                 CGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGT                                                                             >  SRR4302545.1582326/1‑94 (MQ=60)
                 CGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGT                                                                             <  SRR4302545.3454499/94‑1 (MQ=60)
                  GTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTT                                                                            >  SRR4302545.3089369/1‑94 (MQ=60)
                   TTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCTCTCTTTTCTGTTTATGACATGCGCGTGTTT                                                                           <  SRR4302545.8541289/94‑1 (MQ=60)
                   TTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTT                                                                           <  SRR4302545.3950999/94‑1 (MQ=60)
                   TTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTT                                                                           >  SRR4302545.3502110/1‑94 (MQ=60)
                   TTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTT                                                                           <  SRR4302545.3401428/94‑1 (MQ=60)
                   TTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTT                                                                           >  SRR4302545.6671315/1‑94 (MQ=60)
                   TTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTT                                                                           >  SRR4302545.6671314/1‑94 (MQ=60)
                    TATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTG                                                                          >  SRR4302545.9429327/1‑94 (MQ=60)
                    TATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTG                                                                          >  SRR4302545.3668240/1‑94 (MQ=60)
                    TATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTG                                                                          >  SRR4302545.3668239/1‑94 (MQ=60)
                    TATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTG                                                                          >  SRR4302545.9429325/1‑94 (MQ=60)
                     ATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGT                                                                         <  SRR4302545.2289482/94‑1 (MQ=60)
                     ATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGT                                                                         <  SRR4302545.2699475/94‑1 (MQ=60)
                     ATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGT                                                                         >  SRR4302545.9209162/1‑94 (MQ=60)
                       AATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAT                                                                       >  SRR4302545.4976581/1‑94 (MQ=60)
                       AATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTAT                                                                       <  SRR4302545.5801540/94‑1 (MQ=60)
                        ATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATA                                                                      >  SRR4302545.4785037/1‑94 (MQ=60)
                        ATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATA                                                                      <  SRR4302545.5472707/94‑1 (MQ=60)
                        ATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATA                                                                      <  SRR4302545.4061143/94‑1 (MQ=60)
                        ATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATA                                                                      <  SRR4302545.10706292/94‑1 (MQ=60)
                         TGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAA                                                                     <  SRR4302545.8940416/94‑1 (MQ=60)
                         TGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAA                                                                     >  SRR4302545.1896939/1‑94 (MQ=60)
                         TGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAA                                                                     <  SRR4302545.10904600/94‑1 (MQ=60)
                         TGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAA                                                                     <  SRR4302545.7475628/94‑1 (MQ=60)
                         TGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAA                                                                     <  SRR4302545.10904602/94‑1 (MQ=60)
                          GCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAA                                                                    <  SRR4302545.9602046/94‑1 (MQ=60)
                            CTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATG                                                                  <  SRR4302545.193712/94‑1 (MQ=60)
                            CTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATG                                                                  <  SRR4302545.5013176/94‑1 (MQ=60)
                            CTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATG                                                                  >  SRR4302545.6896597/1‑94 (MQ=60)
                             TGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGT                                                                 >  SRR4302545.500993/1‑94 (MQ=60)
                             TGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGT                                                                 <  SRR4302545.478641/94‑1 (MQ=60)
                              GAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTA                                                                <  SRR4302545.2706872/94‑1 (MQ=60)
                               AAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAA                                                               <  SRR4302545.1716734/94‑1 (MQ=60)
                               AAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAA                                                               <  SRR4302545.4578769/94‑1 (MQ=60)
                                AGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAA                                                              >  SRR4302545.2026502/1‑94 (MQ=60)
                                AGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAA                                                              <  SRR4302545.3373399/94‑1 (MQ=60)
                                AGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAA                                                              <  SRR4302545.4103128/94‑1 (MQ=60)
                                AGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAA                                                              >  SRR4302545.604392/1‑94 (MQ=60)
                                 GTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAAT                                                             <  SRR4302545.8151117/94‑1 (MQ=60)
                                 GTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAAT                                                             >  SRR4302545.8873883/1‑94 (MQ=60)
                                 GTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAAT                                                             <  SRR4302545.5804567/94‑1 (MQ=60)
                                  TAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATG                                                            <  SRR4302545.8381817/94‑1 (MQ=60)
                                    GATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTG                                                          <  SRR4302545.8125903/94‑1 (MQ=60)
                                    GATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTG                                                          <  SRR4302545.9624115/94‑1 (MQ=60)
                                      TCACAGAATATATCTTCAGGGATCGCATATCTATTAATTTACTCACTTTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAG                                                        <  SRR4302545.3506848/94‑1 (MQ=60)
                                      TCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAG                                                        <  SRR4302545.8804588/94‑1 (MQ=60)
                                      GCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAG                                                        <  SRR4302545.8804589/94‑1 (MQ=60)
                                       CACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGT                                                       >  SRR4302545.9842530/1‑94 (MQ=60)
                                        ACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTC                                                      >  SRR4302545.6367602/1‑94 (MQ=60)
                                        ACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTC                                                      <  SRR4302545.9419974/94‑1 (MQ=60)
                                          AGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCT                                                    >  SRR4302545.580624/1‑94 (MQ=60)
                                          AGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCT                                                    >  SRR4302545.3250202/1‑94 (MQ=60)
                                           GAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTT                                                   <  SRR4302545.9793509/94‑1 (MQ=60)
                                           GAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTT                                                   >  SRR4302545.1042729/1‑94 (MQ=60)
                                            AATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTG                                                  >  SRR4302545.7892388/1‑94 (MQ=60)
                                             ATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGT                                                 >  SRR4302545.1880001/1‑94 (MQ=60)
                                               ATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTC                                               >  SRR4302545.5619106/1‑94 (MQ=60)
                                               ATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTC                                               >  SRR4302545.1103719/1‑94 (MQ=60)
                                               ATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTC                                               >  SRR4302545.8023725/1‑94 (MQ=60)
                                                TATCTTCAGGCATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCC                                              <  SRR4302545.9538016/94‑1 (MQ=60)
                                                 GTCTTCCGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGAGTGTTTGTATAAATGTCAATGTGAGTCCTTGTTCCA                                             >  SRR4302545.10874572/1‑94 (MQ=60)
                                                 ATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCA                                             <  SRR4302545.8453659/94‑1 (MQ=60)
                                                  TCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAC                                            >  SRR4302545.2970216/1‑94 (MQ=60)
                                                  TCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAC                                            <  SRR4302545.7928691/94‑1 (MQ=60)
                                                    TTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTC                                          <  SRR4302545.705757/94‑1 (MQ=60)
                                                    TTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTC                                          >  SRR4302545.780518/1‑94 (MQ=60)
                                                     TCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCT                                         <  SRR4302545.6520650/94‑1 (MQ=60)
                                                     TCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCT                                         >  SRR4302545.1949975/1‑94 (MQ=60)
                                                     TCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCT                                         >  SRR4302545.2566705/1‑94 (MQ=60)
                                                     TCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCT                                         <  SRR4302545.9625877/94‑1 (MQ=60)
                                                      CAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTC                                        <  SRR4302545.9562766/94‑1 (MQ=60)
                                                       AGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCG                                       >  SRR4302545.6887847/1‑94 (MQ=60)
                                                       AGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCG                                       <  SRR4302545.2729067/94‑1 (MQ=60)
                                                       AGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCG                                       <  SRR4302545.7424827/94‑1 (MQ=60)
                                                       AGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCG                                       <  SRR4302545.1814802/94‑1 (MQ=60)
                                                        GGGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGT                                      <  SRR4302545.7244164/94‑1 (MQ=60)
                                                         GGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTG                                     <  SRR4302545.4376537/94‑1 (MQ=60)
                                                         GGATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTG                                     <  SRR4302545.4374407/94‑1 (MQ=60)
                                                          GATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGC                                    <  SRR4302545.5426828/94‑1 (MQ=60)
                                                           ATCGCATATTTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGGTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCGCGTGCA                                   >  SRR4302545.9670657/1‑94 (MQ=60)
                                                           ATCGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCA                                   <  SRR4302545.4655589/94‑1 (MQ=60)
                                                            ACGCATATCTATGAAGTTACTCACTCTATTCTGTTTATGACATGCGCGTGTGCGTATTAACGTAAATGTGAGTCCTTGTTCCACTCTCGCGCAG                                  >  SRR4302545.9632785/1‑94 (MQ=60)
                                                             CGCATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGC                                 <  SRR4302545.1995706/94‑1 (MQ=60)
                                                               CATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCAT                               >  SRR4302545.9799197/1‑94 (MQ=60)
                                                               CATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCAT                               >  SRR4302545.4526159/1‑94 (MQ=60)
                                                               CATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCAT                               >  SRR4302545.9799196/1‑94 (MQ=60)
                                                                ATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATC                              >  SRR4302545.236355/1‑94 (MQ=60)
                                                                ATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATC                              >  SRR4302545.2617196/1‑94 (MQ=60)
                                                                ATATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATC                              <  SRR4302545.6380217/94‑1 (MQ=60)
                                                                  ATCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGC                            >  SRR4302545.7845238/1‑94 (MQ=60)
                                                                   TCTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCT                           <  SRR4302545.7876569/94‑1 (MQ=60)
                                                                    CTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTG                          <  SRR4302545.139213/94‑1 (MQ=60)
                                                                    CTATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTG                          <  SRR4302545.1697225/94‑1 (MQ=60)
                                                                     TATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGG                         >  SRR4302545.7683760/1‑94 (MQ=60)
                                                                     TATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGG                         <  SRR4302545.906962/94‑1 (MQ=60)
                                                                     TATTAAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGG                         <  SRR4302545.5905668/94‑1 (MQ=60)
                                                                         AAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCAT                     <  SRR4302545.8760647/94‑1 (MQ=60)
                                                                         AAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCAT                     <  SRR4302545.5161572/94‑1 (MQ=60)
                                                                         AAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCAT                     <  SRR4302545.7959773/94‑1 (MQ=60)
                                                                         AAGTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCAT                     <  SRR4302545.9828507/94‑1 (MQ=60)
                                                                           GTTACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGCCATAC                   >  SRR4302545.3066522/1‑94 (MQ=60)
                                                                           GTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATAC                   >  SRR4302545.1673965/1‑94 (MQ=60)
                                                                             TACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGC                 <  SRR4302545.1143234/94‑1 (MQ=60)
                                                                             TACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGC                 <  SRR4302545.1143233/94‑1 (MQ=60)
                                                                              TCTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCG                <  SRR4302545.508845/94‑1 (MQ=60)
                                                                              ACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCG                <  SRR4302545.4117216/94‑1 (MQ=60)
                                                                              ACTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCG                <  SRR4302545.9170447/94‑1 (MQ=60)
                                                                               CTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGA               >  SRR4302545.5784642/1‑94 (MQ=60)
                                                                               CTCACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGA               >  SRR4302545.10511106/1‑94 (MQ=60)
                                                                                 CCCTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAAC             <  SRR4302545.10873559/94‑1 (MQ=60)
                                                                                 CACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAAC             >  SRR4302545.8707187/1‑94 (MQ=60)
                                                                                 CACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAAC             >  SRR4302545.1184459/1‑94 (MQ=60)
                                                                                  ACTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACA            <  SRR4302545.8015329/94‑1 (MQ=60)
                                                                                   CTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACAC           <  SRR4302545.8611612/94‑1 (MQ=60)
                                                                                   CTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACAC           >  SRR4302545.8275973/1‑94 (MQ=60)
                                                                                   CTCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACAC           >  SRR4302545.8275968/1‑94 (MQ=60)
                                                                                    TCTTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACG          >  SRR4302545.6018986/1‑94 (MQ=60)
                                                                                    TCTTTTCTGTTTATGACATGCGCCTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACG          <  SRR4302545.5962017/94‑1 (MQ=60)
                                                                                    TCTTTTCTGTTTATGACATGCGCCTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACG          <  SRR4302545.5962019/94‑1 (MQ=60)
                                                                                      TTTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTA        <  SRR4302545.2065081/94‑1 (MQ=60)
                                                                                       TTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTAC       >  SRR4302545.8725465/1‑94 (MQ=60)
                                                                                       TTTCTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTAC       <  SRR4302545.10337120/94‑1 (MQ=60)
                                                                                          CTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACCAA    >  SRR4302545.4956380/1‑94 (MQ=60)
                                                                                          CTGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACCAA    >  SRR4302545.9848915/1‑94 (MQ=60)
                                                                                           TGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACCAAC   >  SRR4302545.10234741/1‑94 (MQ=60)
                                                                                           TGTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACCAAC   >  SRR4302545.6622062/1‑94 (MQ=60)
                                                                                            GTTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACCAACA  <  SRR4302545.4851937/94‑1 (MQ=60)
                                                                                            |                                                                                             
AGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTCTCGTGCAGCATCGCTGGTCATACGCGAACACGTACCAACA  >  NC_000913/2404805‑2404990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑