Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,443,258 A→G V108A (GTC→GCC)  yfcA ← putative structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,443,2580AG98.9% 908.7 / ‑1.2 264V108A (GTC→GCC) yfcAputative structural protein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (144/117);  total (144/120)
Fisher's exact test for biased strand distribution p-value = 9.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 270 total aligned reads displayed.

CGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAA  >  NC_000913/2443168‑2443348
                                                                                          |                                                                                          
cGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCAt                                                                                         <  1:4751149/94‑1 (MQ=255)
   cgcTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAg                                                                                      <  1:3507563/94‑1 (MQ=255)
   cgcTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAg                                                                                      >  1:1102057/1‑94 (MQ=255)
    gcTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGc                                                                                     >  2:4975583/1‑94 (MQ=255)
    gcTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGc                                                                                     >  2:3159867/1‑94 (MQ=255)
       ggCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTg                                                                                  <  1:7820864/94‑1 (MQ=255)
        gCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGa                                                                                 <  2:3524706/94‑1 (MQ=255)
         cGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGaa                                                                                >  2:7884345/1‑94 (MQ=255)
         cGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGaa                                                                                <  1:2387241/94‑1 (MQ=255)
          ggTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAAc                                                                               <  2:7583474/94‑1 (MQ=255)
          ggTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAAc                                                                               <  1:1164567/94‑1 (MQ=255)
          ggTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAAc                                                                               >  1:3730145/1‑94 (MQ=255)
          ggTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAAc                                                                               >  2:2264044/1‑94 (MQ=255)
             cttcttCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTa                                                                            >  2:5117426/1‑94 (MQ=255)
              ttcttcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTAt                                                                           <  1:7728169/94‑1 (MQ=255)
              ttcttcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAAAGTAt                                                                           >  1:909599/1‑94 (MQ=255)
                cttcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTg                                                                         >  1:3843960/1‑94 (MQ=255)
                cttcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTg                                                                         >  1:8210169/1‑94 (MQ=255)
                cttcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTg                                                                         >  2:8881313/1‑94 (MQ=255)
                cttcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTg                                                                         >  2:7455976/1‑94 (MQ=255)
                 ttcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGc                                                                        >  2:10491790/1‑94 (MQ=255)
                 ttcACCTAGCTTTGGCAGCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGCCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGc                                                                        <  1:8742897/94‑1 (MQ=255)
                  tcACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCa                                                                       >  2:6642130/1‑94 (MQ=255)
                   cACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCAc                                                                      >  1:9290626/1‑94 (MQ=255)
                   cACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCAc                                                                      >  1:6413923/1‑94 (MQ=255)
                    aCCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCAcc                                                                     <  1:6233975/94‑1 (MQ=255)
                     ccTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACca                                                                    >  2:7224150/1‑94 (MQ=255)
                       tAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACcagc                                                                  >  2:4469008/1‑94 (MQ=255)
                       tAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACcagc                                                                  <  1:6543225/94‑1 (MQ=255)
                       tAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGAATCAGCCTGAACGTATTGCACcagc                                                                  >  1:9523962/1‑94 (MQ=255)
                        aGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACcagca                                                                 <  2:6178580/94‑1 (MQ=255)
                         gCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACcagcag                                                                <  1:10339179/94‑1 (MQ=255)
                         gCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACcagcag                                                                >  1:1225246/1‑94 (MQ=255)
                         gCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACcagcag                                                                >  1:10484463/1‑94 (MQ=255)
                          cTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGt                                                               >  2:118911/1‑94 (MQ=255)
                          cTTGGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGt                                                               <  2:5739508/94‑1 (MQ=255)
                             tGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTgcg                                                            <  2:2479440/94‑1 (MQ=255)
                             tGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTgcg                                                            >  1:2871973/1‑94 (MQ=255)
                               gCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGcgcc                                                          >  2:5649016/1‑94 (MQ=255)
                                cATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGcgccg                                                         >  2:10574768/1‑94 (MQ=255)
                                   cAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTc                                                      >  2:8193117/1‑94 (MQ=255)
                                   cAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTc                                                      >  2:10183267/1‑94 (MQ=255)
                                    aGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCa                                                     >  2:8367207/1‑94 (MQ=255)
                                     gaaaGAAATAGAGGCCGATACAAATTACCAGAATGAGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCAt                                                    <  1:10065739/92‑1 (MQ=255)
                                     gCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCAt                                                    <  1:9202221/94‑1 (MQ=255)
                                     gCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCCCTCAt                                                    <  1:3071499/94‑1 (MQ=255)
                                      cAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCAtt                                                   <  1:508959/94‑1 (MQ=255)
                                        aGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGa                                                 <  2:8706733/94‑1 (MQ=255)
                                            aTAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCg                                             <  1:6692725/94‑1 (MQ=255)
                                              agagGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGAc                                           <  2:588931/94‑1 (MQ=255)
                                                agGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCGTTGAGCCGACaa                                         >  1:9024454/1‑94 (MQ=255)
                                                agGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCACTGAGCCGACaa                                         >  1:5328056/1‑94 (MQ=255)
                                                 ggCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACaaa                                        <  1:6190017/94‑1 (MQ=255)
                                                 ggCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACaaa                                        >  2:9210467/1‑94 (MQ=255)
                                                 ggCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACaaa                                        <  1:5202640/94‑1 (MQ=255)
                                                  gCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAg                                       <  1:3170014/94‑1 (MQ=255)
                                                  gCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAg                                       >  1:8507716/1‑94 (MQ=255)
                                                   ccGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAgg                                      >  1:735089/1‑94 (MQ=255)
                                                     gATACAAATTCCCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTc                                    <  1:2316506/94‑1 (MQ=255)
                                                     gATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTc                                    <  2:5449355/94‑1 (MQ=255)
                                                      aTACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCa                                   <  2:409320/94‑1 (MQ=255)
                                                      aTACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCa                                   >  2:10451832/1‑94 (MQ=255)
                                                      aTAAAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCa                                   <  2:3426317/94‑1 (MQ=255)
                                                        aCAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATg                                 <  2:5822060/94‑1 (MQ=255)
                                                          aaaTTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGc                               <  2:2969049/94‑1 (MQ=255)
                                                          aaaTTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGc                               >  1:1535779/1‑94 (MQ=255)
                                                           aaTTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCg                              >  1:7689798/1‑94 (MQ=255)
                                                           aaTTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCg                              >  1:5718547/1‑94 (MQ=255)
                                                           aaTTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCg                              >  2:6260128/1‑94 (MQ=255)
                                                           aaTTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCg                              <  2:8616644/94‑1 (MQ=255)
                                                              tACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGata                           >  2:235676/1‑94 (MQ=255)
                                                               aCCAGAATGGGCACAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGAGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGatct                          >  1:8283101/1‑92 (MQ=255)
                                                                ccAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATAtt                         <  1:6733925/94‑1 (MQ=255)
                                                                  aGAATGGTAAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGa                       >  1:5885952/1‑94 (MQ=255)
                                                                   gAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAg                      <  1:1975785/94‑1 (MQ=255)
                                                                   gAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAg                      <  1:9975389/94‑1 (MQ=255)
                                                                    aaTGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGt                     >  2:7073573/1‑94 (MQ=255)
                                                                    aaTGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGt                     >  2:7052075/1‑94 (MQ=255)
                                                                     aTGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGtt                    >  1:7521418/1‑94 (MQ=255)
                                                                     aTGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGtt                    <  2:4773277/94‑1 (MQ=255)
                                                                       gggCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTc                  >  1:1750861/1‑94 (MQ=255)
                                                                       gggCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTc                  <  2:3711082/94‑1 (MQ=255)
                                                                         gCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTg                <  1:2126504/94‑1 (MQ=255)
                                                                           aaaaTCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGAt              >  1:191629/1‑94 (MQ=255)
                                                                           aaaaTCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGAt              <  1:4332860/94‑1 (MQ=255)
                                                                           aaaaTCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGAt              >  2:4987222/1‑94 (MQ=255)
                                                                            aaaTCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATc             <  2:3083571/94‑1 (MQ=255)
                                                                            aaaTCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATc             <  1:7022740/94‑1 (MQ=255)
                                                                             aaTCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCg            >  2:1602832/1‑94 (MQ=255)
                                                                             aaTCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCg            >  1:4467693/1‑94 (MQ=255)
                                                                              aTCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGc           >  2:4418116/1‑94 (MQ=255)
                                                                                cTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCtt         <  1:7124487/94‑1 (MQ=255)
                                                                                 tGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTa        >  2:4206445/1‑94 (MQ=255)
                                                                                    cGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAAc     >  1:2620174/1‑94 (MQ=255)
                                                                                    cGTAAGGCATCAGCCTGAAAGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAAc     >  1:10368048/1‑94 (MQ=255)
                                                                                     gTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACt    <  2:5911598/94‑1 (MQ=255)
                                                                                      tAAGTCATCAGCCTGAGCGTATTGCCCCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTa   <  1:1375935/94‑1 (MQ=255)
                                                                                      tAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTa   >  2:6684468/1‑94 (MQ=255)
                                                                                       aaGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTaa  <  2:7721153/94‑1 (MQ=255)
                                                                                       aaGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTaa  <  2:9426208/94‑1 (MQ=255)
                                                                                          |                                                                                          
CGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAA  >  NC_000913/2443168‑2443348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCA  >  NC_000913/2443165‑2443351
                                                                                             |                                                                                             
ATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGG                                                                                               >  SRR4302545.6922558/1‑94 (MQ=60)
ATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGG                                                                                               <  SRR4302545.9373724/94‑1 (MQ=60)
ATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGG                                                                                               >  SRR4302545.3366133/1‑94 (MQ=60)
 TCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGC                                                                                              <  SRR4302545.8730244/94‑1 (MQ=60)
 TCCGGCGCTGGCGCTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGC                                                                                              <  SRR4302545.6773632/94‑1 (MQ=60)
   CGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCAT                                                                                            <  SRR4302545.4872502/94‑1 (MQ=60)
   CGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCAT                                                                                            <  SRR4302545.9674115/94‑1 (MQ=60)
    GGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATC                                                                                           <  SRR4302545.7825419/94‑1 (MQ=60)
     GCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCA                                                                                          <  SRR4302545.1655385/94‑1 (MQ=60)
      CGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAG                                                                                         >  SRR4302545.1126029/1‑94 (MQ=60)
      CGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAG                                                                                         >  SRR4302545.1126030/1‑94 (MQ=60)
      CGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAG                                                                                         <  SRR4302545.3593579/94‑1 (MQ=60)
        CTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCC                                                                                       >  SRR4302545.5777633/1‑94 (MQ=60)
        CTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCC                                                                                       <  SRR4302545.2712401/94‑1 (MQ=60)
        CTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCC                                                                                       <  SRR4302545.1902590/94‑1 (MQ=60)
         ttgcgggctccccCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATTCAAATTACCAGAATGGGCAAAATCTTCCGTAAGGCAACAGCCT                                                                                      >  SRR4302545.9687257/14‑94 (MQ=60)
         TGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCT                                                                                      <  SRR4302545.9474103/94‑1 (MQ=60)
          GGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTG                                                                                     <  SRR4302545.8042414/94‑1 (MQ=60)
            CGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAA                                                                                   <  SRR4302545.2445158/94‑1 (MQ=60)
             GGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAAC                                                                                  >  SRR4302545.3821865/1‑94 (MQ=60)
             GGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAAC                                                                                  <  SRR4302545.1190280/94‑1 (MQ=60)
              GTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACG                                                                                 >  SRR4302545.866520/1‑94 (MQ=60)
                 TTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTAT                                                                              <  SRR4302545.10055380/94‑1 (MQ=60)
                 TTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTAT                                                                              <  SRR4302545.1823081/94‑1 (MQ=60)
                 TTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTAT                                                                              >  SRR4302545.6161276/1‑94 (MQ=60)
                 TTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTAT                                                                              <  SRR4302545.7946907/94‑1 (MQ=60)
                 TTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAAAGTAT                                                                              >  SRR4302545.929993/1‑94 (MQ=60)
                  TCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATT                                                                             >  SRR4302545.5388574/1‑94 (MQ=60)
                   CTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTG                                                                            >  SRR4302545.8447793/1‑94 (MQ=60)
                   CTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTG                                                                            >  SRR4302545.7353887/1‑94 (MQ=60)
                   CTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTG                                                                            >  SRR4302545.3939860/1‑94 (MQ=60)
                    TTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGC                                                                           >  SRR4302545.6587505/1‑94 (MQ=60)
                    TTCACCTAGCTTTGGCAGCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGCCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGC                                                                           <  SRR4302545.8996695/94‑1 (MQ=60)
                      CACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCAC                                                                         >  SRR4302545.9563007/1‑94 (MQ=60)
                      CACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCAC                                                                         >  SRR4302545.6589296/1‑94 (MQ=60)
                      CACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCAC                                                                         <  SRR4302545.2458503/94‑1 (MQ=60)
                       ACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACC                                                                        >  SRR4302545.4470011/1‑94 (MQ=60)
                       ACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACC                                                                        <  SRR4302545.6403871/94‑1 (MQ=60)
                        CCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCA                                                                       >  SRR4302545.3364806/1‑94 (MQ=60)
                          TAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGC                                                                     <  SRR4302545.7964525/94‑1 (MQ=60)
                          TAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGC                                                                     <  SRR4302545.6722220/94‑1 (MQ=60)
                          TAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGAATCAGCCTGAACGTATTGCACCAGC                                                                     >  SRR4302545.9804372/1‑94 (MQ=60)
                           AGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCA                                                                    <  SRR4302545.9412335/94‑1 (MQ=60)
                            GCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAG                                                                   >  SRR4302545.1253003/1‑94 (MQ=60)
                            GCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAG                                                                   >  SRR4302545.10793526/1‑94 (MQ=60)
                            GCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAG                                                                   <  SRR4302545.10643963/94‑1 (MQ=60)
                            GCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAG                                                                   <  SRR4302545.10643962/94‑1 (MQ=60)
                             CTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGT                                                                  >  SRR4302545.1700957/1‑94 (MQ=60)
                              TTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTG                                                                 >  SRR4302545.3991695/1‑94 (MQ=60)
                                TGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCG                                                               >  SRR4302545.2941423/1‑94 (MQ=60)
                                TGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCG                                                               >  SRR4302545.7762441/1‑94 (MQ=60)
                                TGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCG                                                               <  SRR4302545.7543258/94‑1 (MQ=60)
                                  GCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCC                                                             <  SRR4302545.3198569/94‑1 (MQ=60)
                                    ATCAGCAAGAAATAGAGGTCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGGATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGC                                                           <  SRR4302545.9338826/94‑1 (MQ=60)
                                    ATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGC                                                           >  SRR4302545.7722310/1‑94 (MQ=60)
                                     TCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCT                                                          <  SRR4302545.2281269/94‑1 (MQ=60)
                                      CAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTC                                                         >  SRR4302545.7184058/1‑94 (MQ=60)
                                      CAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTC                                                         >  SRR4302545.1152447/1‑94 (MQ=60)
                                      CAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTC                                                         >  SRR4302545.6622969/1‑94 (MQ=60)
                                       AGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCA                                                        >  SRR4302545.1526608/1‑94 (MQ=60)
                                        GCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCAT                                                       <  SRR4302545.9471952/94‑1 (MQ=60)
                                        GCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCCCTCAT                                                       <  SRR4302545.3146837/94‑1 (MQ=60)
                                        GAAAGAAATAGAGGCCGATACAAATTACCAGAATGAGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCAT                                                       <  SRR4302545.10363605/94‑1 (MQ=60)
                                         CAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATT                                                      >  SRR4302545.2420370/1‑94 (MQ=60)
                                         CAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATT                                                      <  SRR4302545.520365/94‑1 (MQ=60)
                                               ATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCG                                                <  SRR4302545.3841001/94‑1 (MQ=60)
                                               ATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCG                                                <  SRR4302545.6876205/94‑1 (MQ=60)
                                               ATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCG                                                <  SRR4302545.6876204/94‑1 (MQ=60)
                                                TAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGA                                               >  SRR4302545.5791570/1‑94 (MQ=60)
                                                  GAGGCCGATACACATTAACAGAGTGGCCAAAATCTGCCGTAAGGCACGAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACA                                             <  SRR4302545.6822819/94‑1 (MQ=60)
                                                  GAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACA                                             >  SRR4302545.1242010/1‑94 (MQ=60)
                                                  GAGGCCGATACAAATTACCAGAATGGGCAAAATCAGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACA                                             >  SRR4302545.1242009/1‑94 (MQ=60)
                                                   AGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCGTTGAGCCGACAA                                            >  SRR4302545.9287349/1‑94 (MQ=60)
                                                   AGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCACTGAGCCGACAA                                            >  SRR4302545.5466351/1‑94 (MQ=60)
                                                    GGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAA                                           <  SRR4302545.5336657/94‑1 (MQ=60)
                                                    GGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAA                                           <  SRR4302545.6358470/94‑1 (MQ=60)
                                                     GCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAG                                          <  SRR4302545.3247841/94‑1 (MQ=60)
                                                     GCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAG                                          >  SRR4302545.8753835/1‑94 (MQ=60)
                                                     GCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAG                                          >  SRR4302545.8753530/1‑94 (MQ=60)
                                                     GCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAG                                          >  SRR4302545.787498/1‑94 (MQ=60)
                                                      CCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGG                                         <  SRR4302545.3275512/94‑1 (MQ=60)
                                                      CCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGG                                         >  SRR4302545.751619/1‑94 (MQ=60)
                                                      CCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGG                                         <  SRR4302545.5065165/94‑1 (MQ=60)
                                                        GATACAAATTCCCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTC                                       <  SRR4302545.2372926/94‑1 (MQ=60)
                                                        GATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTC                                       >  SRR4302545.9245223/1‑94 (MQ=60)
                                                          TACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCAT                                     <  SRR4302545.9463829/94‑1 (MQ=60)
                                                           ACAAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATG                                    <  SRR4302545.156136/94‑1 (MQ=60)
                                                             AAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGC                                  >  SRR4302545.1571739/1‑94 (MQ=60)
                                                             AAATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGC                                  >  SRR4302545.4144668/1‑94 (MQ=60)
                                                              AATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCG                                 >  SRR4302545.7907519/1‑94 (MQ=60)
                                                              AATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCG                                 >  SRR4302545.5871919/1‑94 (MQ=60)
                                                              AATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCG                                 <  SRR4302545.3660038/94‑1 (MQ=60)
                                                              AATTACCAGAATGGGCAAAATCTGCCGTAAAGCATCAGCCTTAACGTATTGCACCAGCAGTGCGCCGCTCACTGAGTCGACAAAGGTCATGGCG                                 >  SRR4302545.6375996/1‑94 (MQ=60)
                                                               ATTACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGA                                >  SRR4302545.7220173/1‑94 (MQ=60)
                                                                 TACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATA                              <  SRR4302545.7645012/94‑1 (MQ=60)
                                                                  ACCAGAATGGGCACAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGAGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATCT                             >  SRR4302545.8523044/1‑94 (MQ=60)
                                                                  ACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATAT                             >  SRR4302545.8523043/1‑94 (MQ=60)
                                                                  ACCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATAT                             >  SRR4302545.9512164/1‑94 (MQ=60)
                                                                   CCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATT                            <  SRR4302545.6918544/94‑1 (MQ=60)
                                                                   CCAGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATT                            <  SRR4302545.10605642/94‑1 (MQ=60)
                                                                     AGAATGGTAAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGA                          >  SRR4302545.6044618/1‑94 (MQ=60)
                                                                     AGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGGGATATTGA                          <  SRR4302545.4867043/94‑1 (MQ=60)
                                                                     AGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGA                          >  SRR4302545.4477480/1‑94 (MQ=60)
                                                                     AGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGA                          >  SRR4302545.6044617/1‑94 (MQ=60)
                                                                     AGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGA                          >  SRR4302545.5988370/1‑94 (MQ=60)
                                                                     AGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGA                          >  SRR4302545.2132320/1‑94 (MQ=60)
                                                                     AGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGA                          >  SRR4302545.116459/1‑94 (MQ=60)
                                                                     AGAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGA                          <  SRR4302545.10777169/94‑1 (MQ=60)
                                                                      GAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAG                         <  SRR4302545.10271244/94‑1 (MQ=60)
                                                                      GAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAG                         <  SRR4302545.2024727/94‑1 (MQ=60)
                                                                      GAATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAG                         <  SRR4302545.500826/94‑1 (MQ=60)
                                                                        ATGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTT                       >  SRR4302545.7734200/1‑94 (MQ=60)
                                                                         TGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTT                      <  SRR4302545.3169630/94‑1 (MQ=60)
                                                                         TGGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTT                      >  SRR4302545.6346066/1‑94 (MQ=60)
                                                                          GGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTC                     >  SRR4302545.1792758/1‑94 (MQ=60)
                                                                          GGGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTC                     <  SRR4302545.9327653/94‑1 (MQ=60)
                                                                           GGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCT                    <  SRR4302545.1970858/94‑1 (MQ=60)
                                                                           GGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCT                    >  SRR4302545.8054290/1‑94 (MQ=60)
                                                                           GGCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCT                    <  SRR4302545.7992692/94‑1 (MQ=60)
                                                                            GCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTG                   <  SRR4302545.2178689/94‑1 (MQ=60)
                                                                            GCAAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTG                   <  SRR4302545.8642530/94‑1 (MQ=60)
                                                                              AAAATCTGCCTTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGAT                 <  SRR4302545.987890/94‑1 (MQ=60)
                                                                              AAAATCTGCCGTAAGGCATCGGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTTTGAT                 >  SRR4302545.2984496/1‑94 (MQ=60)
                                                                              AAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGAT                 >  SRR4302545.195667/1‑94 (MQ=60)
                                                                              AAAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGAT                 <  SRR4302545.4442171/94‑1 (MQ=60)
                                                                               AAATCTGCCGTAATGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATC                >  SRR4302545.6454106/1‑94 (MQ=60)
                                                                               AAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATC                >  SRR4302545.105820/1‑94 (MQ=60)
                                                                               AAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATC                <  SRR4302545.7216593/94‑1 (MQ=60)
                                                                               AAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATC                >  SRR4302545.6902348/1‑94 (MQ=60)
                                                                               AAATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATC                <  SRR4302545.2638772/94‑1 (MQ=60)
                                                                                AATCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCG               >  SRR4302545.4581681/1‑94 (MQ=60)
                                                                                  TCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCT             <  SRR4302545.7061720/94‑1 (MQ=60)
                                                                                  TCTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCT             >  SRR4302545.8793433/1‑94 (MQ=60)
                                                                                   CTGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTT            <  SRR4302545.7321981/94‑1 (MQ=60)
                                                                                    TGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTA           >  SRR4302545.2215647/1‑94 (MQ=60)
                                                                                    TGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTA           <  SRR4302545.10761217/94‑1 (MQ=60)
                                                                                    TGCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTA           <  SRR4302545.2940416/94‑1 (MQ=60)
                                                                                     GCCGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAA          >  SRR4302545.8351626/1‑94 (MQ=60)
                                                                                       CGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAAC        >  SRR4302545.2683270/1‑94 (MQ=60)
                                                                                       CGTAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAAC        >  SRR4302545.3646961/1‑94 (MQ=60)
                                                                                       CGTAAGGCATCAGCCTGAAAGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAAC        >  SRR4302545.10673665/1‑94 (MQ=60)
                                                                                        GTAAGGCATCAGCCTGAATGTATTGCACCAGCAGAGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACT       <  SRR4302545.1378399/94‑1 (MQ=60)
                                                                                         TAAGTCATCAGCCTGAGCGTATTGCCCCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTA      <  SRR4302545.1406765/94‑1 (MQ=60)
                                                                                         TAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTA      <  SRR4302545.5232696/94‑1 (MQ=60)
                                                                                         TAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTA      >  SRR4302545.6712538/1‑94 (MQ=60)
                                                                                         TAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTA      >  SRR4302545.2064064/1‑94 (MQ=60)
                                                                                         TAAGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTA      <  SRR4302545.5232697/94‑1 (MQ=60)
                                                                                           AGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGTTCGCTTAAACTAAC    >  SRR4302545.966975/1‑94 (MQ=60)
                                                                                           AGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAAC    >  SRR4302545.902948/1‑94 (MQ=60)
                                                                                           AGGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAAC    >  SRR4302545.8178365/1‑94 (MQ=60)
                                                                                           AGGCATCAGCCTGAACGTATTGCACAAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAAC    >  SRR4302545.4775092/1‑94 (MQ=60)
                                                                                            GGCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACC   <  SRR4302545.8143706/94‑1 (MQ=60)
                                                                                             GCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCA  <  SRR4302545.10420061/94‑1 (MQ=60)
                                                                                             GCATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCA  <  SRR4302545.7255578/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATCCGGCGCTGGCGGTCTTCTTCACCTAGCTTTGGCATCAGCAAGAAATAGAGGCCGATACAAATTACCAGAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCA  >  NC_000913/2443165‑2443351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑