Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,885	T→C	D48G (GAC→GGC)	vacJ ←	lipoprotein precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,885	0	T	C	98.2%	948.7 / 1.1	286	D48G (GAC→GGC)	vacJ	lipoprotein precursor
Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (137/144); total (139/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 293 total aligned reads displayed.

AAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCAC  >  NC_000913/2462794‑2462975

aaGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCt                                                                                          >  2:742931/1‑94 (MQ=255)

     tGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATAc                                                                                     <  1:9889198/94‑1 (MQ=255)

     tGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATAc                                                                                     >  1:9952310/1‑94 (MQ=255)

     tGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATAc                                                                                     <  1:7356967/94‑1 (MQ=255)

      gCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACa                                                                                    >  1:1075131/1‑94 (MQ=255)

       ccAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAt                                                                                   <  1:9299377/94‑1 (MQ=255)

       ccAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAt                                                                                   >  1:2393898/1‑94 (MQ=255)

        cAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAtt                                                                                  >  1:4619306/1‑94 (MQ=255)

        cAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAtt                                                                                  <  1:3466993/94‑1 (MQ=255)

        cAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAtt                                                                                  >  2:7776984/1‑94 (MQ=255)

        cAGTAAACTTCCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAtt                                                                                  <  1:2898955/94‑1 (MQ=255)

         aGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttg                                                                                 <  1:2112348/94‑1 (MQ=255)

         aGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttg                                                                                 >  1:4938216/1‑94 (MQ=255)

         aGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttg                                                                                 >  2:5855374/1‑94 (MQ=255)

          gTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttga                                                                                >  1:5409636/1‑94 (MQ=255)

           tAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaa                                                                               <  1:5309034/94‑1 (MQ=255)

            aaaGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaag                                                                              <  2:2114432/94‑1 (MQ=255)

            aaaGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaag                                                                              >  1:617423/1‑94 (MQ=255)

            aaaGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaaa                                                                              <  2:10602345/94‑2 (MQ=255)

             aaGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACAttgaagt                                                                             <  1:177686/94‑1 (MQ=255)

             aaGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagt                                                                             >  1:7176927/1‑94 (MQ=255)

              aGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagtt                                                                            >  2:1861919/1‑94 (MQ=255)

              aGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagtt                                                                            <  2:3347628/94‑1 (MQ=255)

               gTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttg                                                                           <  1:5143397/94‑1 (MQ=255)

               gTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttg                                                                           >  1:1355403/1‑94 (MQ=255)

                ttGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttga                                                                          >  1:9809788/1‑94 (MQ=255)

                ttGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttga                                                                          <  2:3613913/94‑1 (MQ=255)

                 tGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttgaa                                                                         >  2:869289/1‑94 (MQ=255)

                 tGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttgaa                                                                         <  2:5109237/94‑1 (MQ=255)

                    taaaaCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTgaagttgaagtt                                                                      <  1:7663924/92‑1 (MQ=255)

                    tCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTgaagttgaagtt                                                                      >  2:8431197/1‑94 (MQ=255)

                       aaCCGTTTAGGGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTa                                                                   <  2:2980606/94‑1 (MQ=255)

                        aCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTAc                                                                  >  2:1296954/1‑94 (MQ=255)

                         ccGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACa                                                                 >  1:7783856/1‑94 (MQ=255)

                          cGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGATAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAt                                                                <  2:8031907/94‑1 (MQ=255)

                          cGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAt                                                                <  1:8287789/94‑1 (MQ=255)

                          cGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAt                                                                >  1:4812386/1‑94 (MQ=255)

                          cGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAt                                                                >  2:7608642/1‑94 (MQ=255)

                           gTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATg                                                               <  1:4101231/94‑1 (MQ=255)

                            ttACGCGCCGGGTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGGTGAAGTTGTACATgg                                                              >  2:9379785/1‑94 (MQ=255)

                             tACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGt                                                             <  1:8353903/94‑1 (MQ=255)

                               cgcgCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGc                                                           >  2:432198/1‑94 (MQ=255)

                               cgcgCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGc                                                           <  2:3923857/94‑1 (MQ=255)

                               cgcgCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGc                                                           >  1:1727704/1‑94 (MQ=255)

                                 cgcCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCgg                                                         <  2:89170/94‑1 (MQ=255)

                                   ccGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGtt                                                       <  2:5721779/94‑1 (MQ=255)

                                     ggTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGa                                                     >  2:3736038/1‑94 (MQ=255)

                                     ggTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGa                                                     <  1:6250422/94‑1 (MQ=255)

                                     ggTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTATACATGGTGCGGTTGa                                                     >  2:2539769/1‑94 (MQ=255)

                                        tGCGGAACATAATCACGCCAGGCGACAGCGCCCGGTCGAACAATATACGGGCCTAATACATCGAAGTTGAAGTTGTAAATCGTGCGGTTgcaac                                                  >  2:6102188/1‑90 (MQ=255)

                                        tGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAAcc                                                  >  1:8972205/1‑94 (MQ=255)

                                         gCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAAccc                                                 <  2:5325903/94‑1 (MQ=255)

                                          cGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCt                                                <  2:4114323/94‑1 (MQ=255)

                                          cGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCt                                                <  1:3823359/94‑1 (MQ=255)

                                          cGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCt                                                <  1:3179949/94‑1 (MQ=255)

                                              aCATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTa                                            >  1:4296330/1‑94 (MQ=255)

                                                aTAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAAc                                          >  2:7234756/1‑94 (MQ=255)

                                                aTAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAAc                                          >  1:6342869/1‑94 (MQ=255)

                                                 tAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACg                                         <  1:6448445/94‑1 (MQ=255)

                                                  aaTCACGCCAGGCGAAAGCGACCGGTAGAACAATATACGGGCCTAATACATTGAAGGTGAAGTTGTACATGGTGCGGTTGATCCTTTCAAACgg                                        >  1:5088648/1‑94 (MQ=255)

                                                    tCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGt                                      <  2:657222/94‑1 (MQ=255)

                                                     cACGCCAGGCGACGGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTc                                     <  2:1548971/94‑1 (MQ=255)

                                                     cACGCCAGGCGACAGCGACCGGTCGAACAATATACGTGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTc                                     <  1:5545408/94‑1 (MQ=255)

                                                       cGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAg                                   >  1:2626901/1‑94 (MQ=255)

                                                       cGCCAGGCGACAGCGACCGGCCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAg                                   >  2:10258386/1‑94 (MQ=255)

                                                         ccAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGaa                                 >  2:1211046/1‑94 (MQ=255)

                                                          cAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAc                                >  1:5874162/1‑94 (MQ=255)

                                                           aGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACg                               >  1:6885050/1‑94 (MQ=255)

                                                               gagaGCGACCTGTCGAAAAATATACGGAACTAAGACATTGAAGTTGAAGTTGTACATGGTGCGGTTGATCCCTTCTAACGGGTCAGAACGCCCt                           <  1:9493667/91‑1 (MQ=255)

                                                               gACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCt                           >  1:9550090/1‑94 (MQ=255)

                                                               gACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCt                           >  2:10427320/1‑94 (MQ=255)

                                                                 cAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAGCGGGTCAGAACGCCCttc                         >  2:6829739/1‑93 (MQ=255)

                                                                 cAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTg                         >  1:10446460/1‑94 (MQ=255)

                                                                  aGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGc                        >  1:6717072/1‑94 (MQ=255)

                                                                  aGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGc                        >  1:10290333/1‑94 (MQ=255)

                                                                   gCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCt                       >  2:2204173/1‑94 (MQ=255)

                                                                    cGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTg                      <  2:3915852/94‑1 (MQ=255)

                                                                     gACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGa                     >  1:8708493/1‑94 (MQ=255)

                                                                      aCCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGAt                    >  1:8707818/1‑94 (MQ=255)

                                                                       ccGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATc                   >  1:1885219/1‑94 (MQ=255)

                                                                        cGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCt                  >  2:8865939/1‑94 (MQ=255)

                                                                        cGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCt                  >  1:10073580/1‑94 (MQ=255)

                                                                        cGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCt                  <  2:4562/94‑1 (MQ=255)

                                                                         ggTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCATCTAACGGGTCAGAACGCCCTTGCTGATCTg                 <  2:3422945/94‑1 (MQ=255)

                                                                          gTCTAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGt                <  1:3207599/94‑1 (MQ=255)

                                                                          gTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGt                <  1:9652226/94‑1 (MQ=255)

                                                                            cGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAc              <  1:1022242/94‑1 (MQ=255)

                                                                            cGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAc              >  2:10122007/1‑94 (MQ=255)

                                                                            cGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAc              <  1:7571977/94‑1 (MQ=255)

                                                                             gAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAcc             >  2:954109/1‑94 (MQ=255)

                                                                              aaCAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCg            <  2:1818894/94‑1 (MQ=255)

                                                                                 aaTATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGaa         >  2:8148333/1‑94 (MQ=255)

                                                                                    ataCGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTc      >  2:8559494/1‑94 (MQ=255)

                                                                                     taCGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCg     <  2:6035020/94‑1 (MQ=255)

                                                                                     aaCGGGCCTAATATATTGAAGTTGAAATTGTACATGGTGATGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACACg     <  1:8889533/93‑1 (MQ=255)

                                                                                       cGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGcc   >  2:9757103/1‑93 (MQ=255)

                                                                                        gggCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGcac  >  2:2778765/1‑94 (MQ=255)

                                                                                        gggCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGcac  >  1:10042549/1‑94 (MQ=255)

                                                                                        gggCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGcac  <  2:7291958/94‑1 (MQ=255)

                                                                                        gggCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGcac  >  2:4815904/1‑94 (MQ=255)

AAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCAC  >  NC_000913/2462794‑2462975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACA  >  NC_000913/2462793‑2462976

CAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCC                                                                                            >  SRR4302545.4972744/1‑94 (MQ=60)

CAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCC                                                                                            <  SRR4302545.7560427/94‑1 (MQ=60)

CAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCC                                                                                            >  SRR4302545.1827825/1‑94 (MQ=60)

 AAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCT                                                                                           <  SRR4302545.7508727/94‑1 (MQ=60)

   GGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAA                                                                                         >  SRR4302545.7350133/1‑94 (MQ=60)

    tttgtCAGTAAAGTTGCTCAAGCCGTTACGCGCCGGTTGCTGAATATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAAT                                                                                        <  SRR4302545.6203014/89‑1 (MQ=60)

     TTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATA                                                                                       <  SRR4302545.6785664/94‑1 (MQ=60)

      TGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATAC                                                                                      <  SRR4302545.65133/94‑1 (MQ=60)

      TGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATAC                                                                                      <  SRR4302545.10183016/94‑1 (MQ=60)

      TGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATAC                                                                                      >  SRR4302545.10247656/1‑94 (MQ=60)

      TGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATAC                                                                                      <  SRR4302545.7563880/94‑1 (MQ=60)

      TGCCAGTAAAGTTGCTCAAACCGTTACGAGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATAA                                                                                      >  SRR4302545.10247657/1‑94 (MQ=60)

       GCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACA                                                                                     >  SRR4302545.1098465/1‑94 (MQ=60)

        CCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAT                                                                                    >  SRR4302545.2451973/1‑94 (MQ=60)

        CCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAT                                                                                    <  SRR4302545.9572008/94‑1 (MQ=60)

         CAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATT                                                                                   >  SRR4302545.802864/1‑94 (MQ=60)

         CAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATT                                                                                   <  SRR4302545.3551888/94‑1 (MQ=60)

         CAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATT                                                                                   >  SRR4302545.4737459/1‑94 (MQ=60)

         CAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATT                                                                                   <  SRR4302545.9753872/94‑1 (MQ=60)

         CAGTAAACTTCCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATT                                                                                   <  SRR4302545.2969214/94‑1 (MQ=60)

          AGTAAAGTTGCTCAACCCGTTACGCTCCGGTTGCGGAACATAATCACGCCAGGTGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTG                                                                                  <  SRR4302545.7917573/94‑1 (MQ=60)

          AGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTG                                                                                  >  SRR4302545.5063419/1‑94 (MQ=60)

          AGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTG                                                                                  <  SRR4302545.3726424/94‑1 (MQ=60)

          AGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTG                                                                                  <  SRR4302545.2164226/94‑1 (MQ=60)

           GTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGA                                                                                 >  SRR4302545.9979650/1‑94 (MQ=60)

           GTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGA                                                                                 <  SRR4302545.5536454/94‑1 (MQ=60)

           GTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGA                                                                                 >  SRR4302545.5550777/1‑94 (MQ=60)

            TAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAA                                                                                <  SRR4302545.5446682/94‑1 (MQ=60)

            AAAAGTAGCTCAACCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACGGGTCGAACAATATACGGGCCTAATACATTGAA                                                                                <  SRR4302545.1211286/94‑1 (MQ=60)

             AAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAG                                                                               >  SRR4302545.2842741/1‑94 (MQ=60)

             AAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAG                                                                               >  SRR4302545.631294/1‑94 (MQ=60)

              AAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGT                                                                              <  SRR4302545.181471/94‑1 (MQ=60)

              AAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAGGT                                                                              >  SRR4302545.7795477/1‑94 (MQ=60)

              AAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGT                                                                              >  SRR4302545.7376345/1‑94 (MQ=60)

               AGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTT                                                                             <  SRR4302545.7695636/94‑1 (MQ=60)

               AGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTT                                                                             >  SRR4302545.3265435/1‑94 (MQ=60)

               AGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTT                                                                             >  SRR4302545.878959/1‑94 (MQ=60)

               AGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTT                                                                             >  SRR4302545.10198037/1‑94 (MQ=60)

                GTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTG                                                                            <  SRR4302545.5275526/94‑1 (MQ=60)

                GTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTG                                                                            <  SRR4302545.8770254/94‑1 (MQ=60)

                GTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTG                                                                            >  SRR4302545.1385698/1‑94 (MQ=60)

                GTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTG                                                                            <  SRR4302545.2158694/94‑1 (MQ=60)

                 TTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGA                                                                           >  SRR4302545.10101268/1‑94 (MQ=60)

                  TGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAA                                                                          <  SRR4302545.3233647/94‑1 (MQ=60)

                   GCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAG                                                                         >  SRR4302545.558176/1‑94 (MQ=60)

                   GCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAG                                                                         <  SRR4302545.7040600/94‑1 (MQ=60)

                   GCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAG                                                                         <  SRR4302545.7040601/94‑1 (MQ=60)

                    CTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGT                                                                        <  SRR4302545.2577679/94‑1 (MQ=60)

                     TCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTT                                                                       <  SRR4302545.1029730/94‑1 (MQ=60)

                     TAAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTT                                                                       <  SRR4302545.7880842/94‑1 (MQ=60)

                      CAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTG                                                                      <  SRR4302545.947314/94‑1 (MQ=60)

                       AAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGT                                                                     <  SRR4302545.524510/94‑1 (MQ=60)

                         ACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTAC                                                                   >  SRR4302545.10770856/1‑94 (MQ=60)

                          CCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACA                                                                  >  SRR4302545.8004193/1‑94 (MQ=60)

                           CGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAT                                                                 <  SRR4302545.5670200/94‑1 (MQ=60)

                           CGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAT                                                                 >  SRR4302545.4935316/1‑94 (MQ=60)

                           CGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAT                                                                 <  SRR4302545.8527868/94‑1 (MQ=60)

                            GTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATG                                                                <  SRR4302545.4819932/94‑1 (MQ=60)

                            GTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATG                                                                <  SRR4302545.10167739/94‑1 (MQ=60)

                            GTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATG                                                                >  SRR4302545.3585449/1‑94 (MQ=60)

                            GTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATG                                                                >  SRR4302545.4231533/1‑94 (MQ=60)

                            GTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATG                                                                <  SRR4302545.4203888/94‑1 (MQ=60)

                             TTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGG                                                               <  SRR4302545.1811665/94‑1 (MQ=60)

                             TTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGG                                                               <  SRR4302545.7756677/94‑1 (MQ=60)

                              TACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGT                                                              <  SRR4302545.5151341/94‑1 (MQ=60)

                              TACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGT                                                              <  SRR4302545.8579738/94‑1 (MQ=60)

                              TACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGT                                                              <  SRR4302545.8595905/94‑1 (MQ=60)

                                CGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGC                                                            >  SRR4302545.1768918/1‑94 (MQ=60)

                                CGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGC                                                            <  SRR4302545.1891918/94‑1 (MQ=60)

                                 GCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCG                                                           <  SRR4302545.6479899/94‑1 (MQ=60)

                                 GCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCG                                                           <  SRR4302545.7551527/94‑1 (MQ=60)

                                 GCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCG                                                           <  SRR4302545.9899162/94‑1 (MQ=60)

                                  CGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGG                                                          <  SRR4302545.10199023/94‑1 (MQ=60)

                                   GCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGT                                                         <  SRR4302545.2979213/94‑1 (MQ=60)

                                    CCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTT                                                        <  SRR4302545.7203706/94‑1 (MQ=60)

                                    CCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTT                                                        <  SRR4302545.10516184/94‑1 (MQ=60)

                                     CGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTG                                                       <  SRR4302545.10107519/94‑1 (MQ=60)

                                      GGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGA                                                      <  SRR4302545.6420780/94‑1 (MQ=60)

                                       GTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAA                                                     >  SRR4302545.3992993/1‑94 (MQ=60)

                                       GTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAA                                                     >  SRR4302545.5993162/1‑94 (MQ=60)

                                        TTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAAC                                                    <  SRR4302545.10531607/94‑1 (MQ=60)

                                        TTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAAC                                                    >  SRR4302545.9199726/1‑94 (MQ=60)

                                        GTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAAC                                                    <  SRR4302545.179371/94‑1 (MQ=60)

                                         TGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACC                                                   <  SRR4302545.10038239/94‑1 (MQ=60)

                                         TGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACC                                                   >  SRR4302545.8336016/1‑94 (MQ=60)

                                         TGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACC                                                   >  SRR4302545.9233225/1‑94 (MQ=60)

                                          GCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCC                                                  >  SRR4302545.9789925/1‑94 (MQ=60)

                                           CGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCT                                                 >  SRR4302545.2339933/1‑94 (MQ=60)

                                           CGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCT                                                 <  SRR4302545.3257974/94‑1 (MQ=60)

                                           CGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCT                                                 <  SRR4302545.3918539/94‑1 (MQ=60)

                                            GGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTT                                                <  SRR4302545.9070292/94‑1 (MQ=60)

                                              AACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCT                                              >  SRR4302545.2038796/1‑94 (MQ=60)

                                              AACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCT                                              >  SRR4302545.6374426/1‑94 (MQ=60)

                                              AACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCT                                              >  SRR4302545.6374425/1‑94 (MQ=60)

                                               ACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTA                                             >  SRR4302545.4404448/1‑94 (MQ=60)

                                               ACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTA                                             >  SRR4302545.4404452/1‑94 (MQ=60)

                                                CATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAA                                            <  SRR4302545.4701054/94‑1 (MQ=60)

                                                 ATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAAC                                           <  SRR4302545.4271427/94‑1 (MQ=60)

                                                 ATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAAC                                           >  SRR4302545.3436798/1‑94 (MQ=60)

                                                 ATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAAC                                           >  SRR4302545.6516032/1‑94 (MQ=60)

                                                  TAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACG                                          <  SRR4302545.6624748/94‑1 (MQ=60)

                                                   AATCACGCCAGGCGAAAGCGACCGGTAGAACAATATACGGGCCTAATACATTGAAGGTGAAGTTGTACATGGTGCGGTTGATCCTTTCAAACGG                                         >  SRR4302545.5218410/1‑94 (MQ=60)

                                                     TCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGT                                       <  SRR4302545.4807400/94‑1 (MQ=60)

                                                      CACGCCAGGCGACAGCGACCGGTCGAACAATATACGTGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTC                                      <  SRR4302545.5691877/94‑1 (MQ=60)

                                                       ACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCA                                     <  SRR4302545.7880850/94‑1 (MQ=60)

                                                        CGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAG                                    >  SRR4302545.780971/1‑94 (MQ=60)

                                                        CGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAG                                    >  SRR4302545.2690127/1‑94 (MQ=60)

                                                         GCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGA                                   <  SRR4302545.231169/94‑1 (MQ=60)

                                                         GCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGA                                   <  SRR4302545.10735943/94‑1 (MQ=60)

                                                          CCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAA                                  >  SRR4302545.8586534/1‑94 (MQ=60)

                                                          CCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAA                                  >  SRR4302545.1855670/1‑94 (MQ=60)

                                                           CAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAC                                 >  SRR4302545.6032491/1‑94 (MQ=60)

                                                            AGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACG                                >  SRR4302545.5045677/1‑94 (MQ=60)

                                                            AGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACG                                >  SRR4302545.9510392/1‑94 (MQ=60)

                                                            AGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACG                                >  SRR4302545.7074335/1‑94 (MQ=60)

                                                               CGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCC                             <  SRR4302545.9838126/94‑1 (MQ=60)

                                                               CGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCC                             <  SRR4302545.4117670/94‑1 (MQ=60)

                                                                GAGAGCGACCTGTCGAAAAATATACGGAACTAAGACATTGAAGTTGAAGTTGTACATGGTGCGGTTGATCCCTTCTAACGGGTCAGAACGCCCT                            <  SRR4302545.9773103/94‑1 (MQ=60)

                                                                GACAGCGACCGGTCGACGAAGATACGGGCATAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCT                            <  SRR4302545.10542007/94‑1 (MQ=60)

                                                                GACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCT                            >  SRR4302545.9794224/1‑94 (MQ=60)

                                                                GACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCT                            >  SRR4302545.9831465/1‑94 (MQ=60)

                                                                GACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCT                            >  SRR4302545.5238932/1‑94 (MQ=60)

                                                                 ACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTT                           <  SRR4302545.1614970/94‑1 (MQ=60)

                                                                  CAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTG                          >  SRR4302545.10754307/1‑94 (MQ=60)

                                                                   AGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGC                         >  SRR4302545.10593802/1‑94 (MQ=60)

                                                                   AGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGC                         >  SRR4302545.6901229/1‑94 (MQ=60)

                                                                     CGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTG                       >  SRR4302545.6109320/1‑94 (MQ=60)

                                                                      GACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGCACCCTTCTAACAGGACAGAACGCACTTGCTGA                      >  SRR4302545.1164664/1‑94 (MQ=60)

                                                                      GACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGA                      >  SRR4302545.8961132/1‑94 (MQ=60)

                                                                      GACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGA                      >  SRR4302545.8961131/1‑94 (MQ=60)

                                                                      GACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGA                      >  SRR4302545.3385470/1‑94 (MQ=60)

                                                                      GACCGGTCGAACAATATACGGGCATAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGA                      <  SRR4302545.264756/94‑1 (MQ=60)

                                                                       ACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGAT                     >  SRR4302545.8960438/1‑94 (MQ=60)

                                                                       ACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGAT                     >  SRR4302545.8960437/1‑94 (MQ=60)

                                                                        CCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATC                    <  SRR4302545.1546451/94‑1 (MQ=60)

                                                                        CCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATC                    >  SRR4302545.1931610/1‑94 (MQ=60)

                                                                        CCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATC                    >  SRR4302545.2728687/1‑94 (MQ=60)

                                                                        CCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATC                    <  SRR4302545.6576331/94‑1 (MQ=60)

                                                                         CGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCT                   >  SRR4302545.10371632/1‑94 (MQ=60)

                                                                         CGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCT                   >  SRR4302545.10371634/1‑94 (MQ=60)

                                                                           GTCTAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGT                 <  SRR4302545.3286198/94‑1 (MQ=60)

                                                                           GTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGT                 <  SRR4302545.9937628/94‑1 (MQ=60)

                                                                            TCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTA                <  SRR4302545.5009224/94‑1 (MQ=60)

                                                                             CGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAC               <  SRR4302545.7785973/94‑1 (MQ=60)

                                                                             CGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAC               >  SRR4302545.6503920/1‑94 (MQ=60)

                                                                             CGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAC               <  SRR4302545.5364137/94‑1 (MQ=60)

                                                                             CGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAC               <  SRR4302545.1044728/94‑1 (MQ=60)

                                                                              GAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACC              <  SRR4302545.5119136/94‑1 (MQ=60)

                                                                              GAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACC              >  SRR4302545.5364899/1‑94 (MQ=60)

                                                                               AACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCG             <  SRR4302545.5538793/94‑1 (MQ=60)

                                                                               AACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCG             >  SRR4302545.10769135/1‑94 (MQ=60)

                                                                                  AATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAA          >  SRR4302545.10675570/1‑94 (MQ=60)

                                                                                   ATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAAC         <  SRR4302545.68447/94‑1 (MQ=60)

                                                                                    TATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACT        >  SRR4302545.5694355/1‑94 (MQ=60)

                                                                                     ATACGGGCCTATTACATTGAAGTTGAAGTTGTACATGGAGCGGTTGAACCCTTCTAACGGGTCACAACGCCCTTGCTGATCTGTACCGGAAttg       >  SRR4302545.9310853/1‑91 (MQ=60)

                                                                                      TACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCG      <  SRR4302545.9147891/94‑1 (MQ=60)

                                                                                      AACGGGCCTAATATATTGAAGTTGAAATTGTACATGGTGATGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACACG      <  SRR4302545.9147890/94‑1 (MQ=60)

                                                                                        CGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCA    >  SRR4302545.1280145/1‑94 (MQ=60)

                                                                                         GGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCAC   >  SRR4302545.10339977/1‑94 (MQ=60)

                                                                                         GGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCAC   >  SRR4302545.9599977/1‑94 (MQ=60)

                                                                                          GGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACA  >  SRR4302545.6835488/1‑94 (MQ=60)

                                                                                          GGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACA  <  SRR4302545.1271888/94‑1 (MQ=60)

                                                                                          GGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACA  >  SRR4302545.2885203/1‑94 (MQ=60)

CAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACA  >  NC_000913/2462793‑2462976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`