Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,885 T→C D48G (GAC→GGC)  vacJ ← lipoprotein precursor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,8850TC100.0% 694.7 / NA 201D48G (GAC→GGC) vacJlipoprotein precursor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (93/108);  total (93/108)

Only 100 of 205 total aligned reads displayed.

AGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCA  >  NC_000913/2462795‑2462974
                                                                                          |                                                                                         
aGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCt                                                                                         >  1:5359036/1‑93 (MQ=255)
aGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGCTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACAGGCCt                                                                                         >  1:2553307/1‑93 (MQ=255)
 ggTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTa                                                                                        >  2:86119/1‑93 (MQ=255)
 ggTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTa                                                                                        >  2:555328/1‑93 (MQ=255)
  gTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTaa                                                                                       >  1:5788070/1‑93 (MQ=255)
  gTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTaa                                                                                       <  1:2865946/93‑1 (MQ=255)
      cgaGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACa                                                                                   <  2:2516825/91‑1 (MQ=255)
      ccAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACa                                                                                   >  2:3058330/1‑93 (MQ=255)
       cAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAt                                                                                  >  2:5647506/1‑93 (MQ=255)
        aGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAtt                                                                                 >  2:4305033/1‑93 (MQ=255)
         gTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttg                                                                                <  2:1454938/93‑1 (MQ=255)
          tAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttga                                                                               <  1:5120777/93‑1 (MQ=255)
          tAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttga                                                                               <  1:4033354/93‑1 (MQ=255)
          tAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttga                                                                               >  1:6137830/1‑93 (MQ=255)
           aaaGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaa                                                                              >  2:1424859/1‑93 (MQ=255)
            aaGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgacg                                                                             >  1:1650477/1‑91 (MQ=255)
            aaGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaag                                                                             <  2:3835408/93‑1 (MQ=255)
             aGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagt                                                                            <  2:4782096/93‑1 (MQ=255)
             aGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagt                                                                            >  2:882292/1‑93 (MQ=255)
              gTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagtt                                                                           >  2:4223153/1‑93 (MQ=255)
               ttGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttg                                                                          >  1:893842/1‑93 (MQ=255)
               ttGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttg                                                                          >  2:4274227/1‑93 (MQ=255)
                tGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttga                                                                         <  1:724234/93‑1 (MQ=255)
                tGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAttgaagttga                                                                         >  1:4154079/1‑93 (MQ=255)
                    cAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTgaagttgaagtt                                                                     <  1:5773821/93‑1 (MQ=255)
                     aaaCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGaagttgaagttg                                                                    <  1:1124366/93‑1 (MQ=255)
                      aaCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGt                                                                   >  2:5331918/1‑93 (MQ=255)
                      aaCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGt                                                                   <  1:5806074/93‑1 (MQ=255)
                       aCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTa                                                                  <  1:4712694/93‑1 (MQ=255)
                        ccGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTAc                                                                 >  2:6248204/1‑93 (MQ=255)
                         cGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACa                                                                <  2:287562/93‑1 (MQ=255)
                          gTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAt                                                               >  1:4883597/1‑93 (MQ=255)
                          gTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAt                                                               <  1:4200523/93‑1 (MQ=255)
                           ttACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATg                                                              >  1:2001887/1‑93 (MQ=255)
                            tACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATgg                                                             <  1:2432548/93‑1 (MQ=255)
                            tACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATgg                                                             >  2:6461914/1‑93 (MQ=255)
                            tACGCGCCGGTTGCGAAACATAATCACGCAAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATgg                                                             <  1:4529605/93‑1 (MQ=255)
                             aCGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGt                                                            <  1:1229495/93‑1 (MQ=255)
                             aCGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGt                                                            <  1:5023047/93‑1 (MQ=255)
                              cgcgCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTg                                                           <  1:77285/93‑1 (MQ=255)
                               gcgcCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGc                                                          <  2:4025320/93‑1 (MQ=255)
                               gcgcCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGc                                                          <  2:669052/93‑1 (MQ=255)
                               gcgcCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGc                                                          <  2:5984334/93‑1 (MQ=255)
                                cgcCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCg                                                         <  1:1817424/93‑1 (MQ=255)
                                  ccGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGt                                                       >  2:1911598/1‑93 (MQ=255)
                                  ccGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGt                                                       <  2:1679952/93‑1 (MQ=255)
                                   cGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGtt                                                      <  2:3668841/93‑1 (MQ=255)
                                    ggTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTg                                                     >  2:1990356/1‑93 (MQ=255)
                                     gTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGa                                                    >  1:2612730/1‑93 (MQ=255)
                                      ttGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGaa                                                   >  2:4482690/1‑93 (MQ=255)
                                        gCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAAcc                                                 <  2:4122641/93‑1 (MQ=255)
                                           gAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCtt                                              >  2:1377443/1‑93 (MQ=255)
                                            aaCATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCttt                                             >  1:702198/1‑92 (MQ=255)
                                             aCATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCt                                            >  1:3608512/1‑93 (MQ=255)
                                              cATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTa                                           >  2:4104824/1‑93 (MQ=255)
                                              cATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTa                                           <  2:810793/93‑1 (MQ=255)
                                              cATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTa                                           <  2:1783127/93‑1 (MQ=255)
                                               aTAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTaa                                          >  1:2701604/1‑93 (MQ=255)
                                                tAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAAc                                         <  2:4650097/93‑1 (MQ=255)
                                                 aaTCACGCCGGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACg                                        <  2:1154940/93‑1 (MQ=255)
                                                 aaTCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACg                                        <  1:3261829/93‑1 (MQ=255)
                                                  aTCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACgg                                       <  2:4927142/93‑1 (MQ=255)
                                                   tCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACggg                                      <  2:5426655/93‑1 (MQ=255)
                                                     aCGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTc                                    >  2:6117074/1‑93 (MQ=255)
                                                      cGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCa                                   <  2:1043300/93‑1 (MQ=255)
                                                      cGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCa                                   <  1:1535801/93‑1 (MQ=255)
                                                         cAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGaa                                >  1:4829395/1‑93 (MQ=255)
                                                         cAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGaa                                <  2:6432353/93‑1 (MQ=255)
                                                         cAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGaa                                <  1:3008869/93‑1 (MQ=255)
                                                         cAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGaa                                <  1:5465636/93‑1 (MQ=255)
                                                         cAGGCGACAGCGACCGGTCGAACAATATACGGGCATAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGaa                                <  2:1184510/93‑1 (MQ=255)
                                                          aGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAc                               <  1:1359897/93‑1 (MQ=255)
                                                          aGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAc                               >  1:5387914/1‑93 (MQ=255)
                                                          aGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAc                               <  1:2953949/93‑1 (MQ=255)
                                                           ggCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACg                              >  1:6455606/1‑93 (MQ=255)
                                                              gACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGGCAGAACGccc                           >  1:85970/1‑93 (MQ=255)
                                                               aCAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCt                          >  2:5054135/1‑93 (MQ=255)
                                                               aCAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCt                          >  1:1392394/1‑93 (MQ=255)
                                                               aCAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCt                          <  2:3413815/93‑1 (MQ=255)
                                                                  gCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGc                       <  1:5642649/93‑1 (MQ=255)
                                                                   cGACCGGTCGAACAATATACGGGCCTAGTACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCt                      <  1:839647/93‑1 (MQ=255)
                                                                   cGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCt                      <  1:774312/93‑1 (MQ=255)
                                                                   cGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCt                      <  2:5791244/93‑1 (MQ=255)
                                                                     aCCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGa                    >  2:5901458/1‑93 (MQ=255)
                                                                     aCCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGCTGTACATGGTGCGGTTGAACCCTTCTAACCGGTCAGAACGCCCTTGCTGa                    >  1:3454523/1‑93 (MQ=255)
                                                                       cGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATc                  >  2:4770853/1‑93 (MQ=255)
                                                                       cGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATc                  <  1:439995/93‑1 (MQ=255)
                                                                         gTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTg                <  1:107371/93‑1 (MQ=255)
                                                                         gTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTg                >  2:4937971/1‑93 (MQ=255)
                                                                          tCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGt               <  1:2670120/93‑1 (MQ=255)
                                                                           cGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTa              <  2:2598030/93‑1 (MQ=255)
                                                                           cGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTa              >  2:6444794/1‑93 (MQ=255)
                                                                           cGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTa              <  1:5828066/93‑1 (MQ=255)
                                                                            gAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAc             <  2:5316237/93‑1 (MQ=255)
                                                                            gAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAc             <  1:2084642/93‑1 (MQ=255)
                                                                            gAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAc             <  2:1308290/93‑1 (MQ=255)
                                                                                aaTATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGa         >  2:2726049/1‑93 (MQ=255)
                                                                                 atatACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGaa        >  2:4248617/1‑93 (MQ=255)
                                                                                   ataCGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACt      >  2:2086659/1‑93 (MQ=255)
                                                                                       gggCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGca  <  2:1630467/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCA  >  NC_000913/2462795‑2462974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACC  >  NC_000913/2462792‑2462978
                                                                                             |                                                                                             
TCAAGGTTGCCAGTAAAGTTGCTCAAGCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGC                                                                                               <  SRR4302537.2589636/94‑1 (MQ=60)
TCAAGGTTGCCAGTAAAGTTGCTCAACCCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGC                                                                                               <  SRR4302537.3306613/94‑1 (MQ=60)
 CAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCC                                                                                              <  SRR4302537.3499539/94‑1 (MQ=60)
  AAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCT                                                                                             >  SRR4302537.5395734/1‑94 (MQ=60)
  AAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCT                                                                                             <  SRR4302537.4791221/94‑1 (MQ=60)
  AAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGCTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACAGGCCT                                                                                             >  SRR4302537.2571217/1‑94 (MQ=60)
    GGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAA                                                                                           >  SRR4302537.5827762/1‑94 (MQ=60)
    GGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAA                                                                                           >  SRR4302537.4077283/1‑94 (MQ=60)
     GTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAAT                                                                                          <  SRR4302537.2886049/94‑1 (MQ=60)
         CCAGTAAAGTTGCTCAAACCGTTACGCGCCAGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACAT                                                                                      <  SRR4302537.603334/94‑1 (MQ=60)
           AGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTG                                                                                    <  SRR4302537.915747/94‑1 (MQ=60)
            GTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGA                                                                                   <  SRR4302537.1113266/94‑1 (MQ=60)
            GTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGA                                                                                   >  SRR4302537.6180029/1‑94 (MQ=60)
             TAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAA                                                                                  <  SRR4302537.4061582/94‑1 (MQ=60)
             TAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAA                                                                                  <  SRR4302537.5156074/94‑1 (MQ=60)
              AAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGACG                                                                                 >  SRR4302537.1662055/1‑94 (MQ=60)
              AAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAG                                                                                 <  SRR4302537.5344772/94‑1 (MQ=60)
               AAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGT                                                                                >  SRR4302537.4868812/1‑94 (MQ=60)
               AAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGT                                                                                <  SRR4302537.315822/94‑1 (MQ=60)
                 GTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTG                                                                              >  SRR4302537.900009/1‑94 (MQ=60)
                 GTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTG                                                                              <  SRR4302537.759560/94‑1 (MQ=60)
                  TTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGA                                                                             >  SRR4302537.4183085/1‑94 (MQ=60)
                   TGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAA                                                                            <  SRR4302537.87842/94‑1 (MQ=60)
                   TGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAA                                                                            <  SRR4302537.729176/94‑1 (MQ=60)
                      TCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTT                                                                         <  SRR4302537.778971/94‑1 (MQ=60)
                       CAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTG                                                                        <  SRR4302537.5813395/94‑1 (MQ=60)
                       CAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTG                                                                        <  SRR4302537.3553621/94‑1 (MQ=60)
                        AAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGT                                                                       <  SRR4302537.2033167/94‑1 (MQ=60)
                        AAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGT                                                                       <  SRR4302537.1132164/94‑1 (MQ=60)
                         AACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTA                                                                      >  SRR4302537.86317/1‑94 (MQ=60)
                         AACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTA                                                                      <  SRR4302537.5845887/94‑1 (MQ=60)
                          ACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTAC                                                                     <  SRR4302537.4745293/94‑1 (MQ=60)
                           CCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACA                                                                    >  SRR4302537.4476255/1‑94 (MQ=60)
                           CCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACA                                                                    <  SRR4302537.2263961/94‑1 (MQ=60)
                            CGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAT                                                                   <  SRR4302537.5842251/94‑1 (MQ=60)
                            CGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACAT                                                                   >  SRR4302537.4917325/1‑94 (MQ=60)
                             GTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATG                                                                  >  SRR4302537.2015807/1‑94 (MQ=60)
                             GTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATG                                                                  <  SRR4302537.4229851/94‑1 (MQ=60)
                               TACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGT                                                                <  SRR4302537.4649692/94‑1 (MQ=60)
                               TACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGT                                                                <  SRR4302537.2449685/94‑1 (MQ=60)
                               TACGCGCCGGTTGCGAAACATAATCACGCAAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGT                                                                <  SRR4302537.4560969/94‑1 (MQ=60)
                                ACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTG                                                               <  SRR4302537.4313913/94‑1 (MQ=60)
                                ACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTG                                                               <  SRR4302537.5057644/94‑1 (MQ=60)
                                ACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTG                                                               <  SRR4302537.1238046/94‑1 (MQ=60)
                                ACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTG                                                               <  SRR4302537.1182597/94‑1 (MQ=60)
                                 CGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGC                                                              <  SRR4302537.77851/94‑1 (MQ=60)
                                  GCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCG                                                             <  SRR4302537.1354937/94‑1 (MQ=60)
                                   CGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGG                                                            <  SRR4302537.1830089/94‑1 (MQ=60)
                                    GCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGT                                                           >  SRR4302537.1460269/1‑94 (MQ=60)
                                    GCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGT                                                           >  SRR4302537.3913530/1‑94 (MQ=60)
                                       GGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGA                                                        >  SRR4302537.2631008/1‑94 (MQ=60)
                                        GTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAA                                                       >  SRR4302537.3598852/1‑94 (MQ=60)
                                            CGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCT                                                   >  SRR4302537.5435005/1‑94 (MQ=60)
                                            CGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCT                                                   <  SRR4302537.5791006/94‑1 (MQ=60)
                                            CGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCT                                                   <  SRR4302537.2913582/94‑1 (MQ=60)
                                              GAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTT                                                 >  SRR4302537.707007/1‑94 (MQ=60)
                                               AACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCT                                                <  SRR4302537.5136709/94‑1 (MQ=60)
                                               AACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCT                                                >  SRR4302537.3633948/1‑94 (MQ=60)
                                                 CATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAA                                              >  SRR4302537.2720469/1‑94 (MQ=60)
                                                  ATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAAC                                             <  SRR4302537.2713437/94‑1 (MQ=60)
                                                   TAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACG                                            <  SRR4302537.4794174/94‑1 (MQ=60)
                                                    AATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGG                                           <  SRR4302537.3284833/94‑1 (MQ=60)
                                                     ATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGG                                          <  SRR4302537.5562802/94‑1 (MQ=60)
                                                         CGCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAG                                      <  SRR4302537.1546527/94‑1 (MQ=60)
                                                          GCCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGA                                     >  SRR4302537.3024306/1‑94 (MQ=60)
                                                           CCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAA                                    <  SRR4302537.1925285/94‑1 (MQ=60)
                                                           CCAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAA                                    >  SRR4302537.4862754/1‑94 (MQ=60)
                                                            CAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAC                                   <  SRR4302537.3397243/94‑1 (MQ=60)
                                                            CAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAC                                   <  SRR4302537.3029811/94‑1 (MQ=60)
                                                            CAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAC                                   <  SRR4302537.5502917/94‑1 (MQ=60)
                                                            CAGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAAC                                   >  SRR4302537.5424735/1‑94 (MQ=60)
                                                             AGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACG                                  >  SRR4302537.6499367/1‑94 (MQ=60)
                                                             AGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACG                                  >  SRR4302537.2812763/1‑94 (MQ=60)
                                                             AGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACG                                  <  SRR4302537.1369365/94‑1 (MQ=60)
                                                             AGGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACG                                  <  SRR4302537.2974677/94‑1 (MQ=60)
                                                              GGCGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGC                                 <  SRR4302537.2627363/94‑1 (MQ=60)
                                                                CGACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGGCAGAACGCCC                               >  SRR4302537.86596/1‑94 (MQ=60)
                                                                CGACAGCGACCGGCCGATCATTAAACGTGCCTAATACATTGAAGTTGAAGTTGTACATGGAGCGGTTGAACCCTTtattcgggtctgaatgtcc                               >  SRR4302537.6514939/1‑75 (MQ=41)
                                                                 GACAGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCT                              >  SRR4302537.1402101/1‑94 (MQ=60)
                                                                    AGCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGC                           >  SRR4302537.1723591/1‑94 (MQ=60)
                                                                     GCGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCT                          <  SRR4302537.5681263/94‑1 (MQ=60)
                                                                      CGACCGGTCGAACAATATACGGGCCTAGTACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTG                         <  SRR4302537.845400/94‑1 (MQ=60)
                                                                      CGACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTG                         <  SRR4302537.779615/94‑1 (MQ=60)
                                                                       GACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGA                        <  SRR4302537.4976883/94‑1 (MQ=60)
                                                                       GACCGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGCTGTACATGGTGCGGTTGAACCCTTCTAACCGGTCAGAACGCCCTTGCTGA                        >  SRR4302537.3478954/1‑94 (MQ=60)
                                                                         ccgtaCGAACAATATACGGGCCTAATGCATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATC                      <  SRR4302537.1130662/89‑1 (MQ=60)
                                                                          CGGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCT                     <  SRR4302537.443053/94‑1 (MQ=60)
                                                                           GGTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTG                    >  SRR4302537.6307283/1‑94 (MQ=60)
                                                                            GTCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGT                   <  SRR4302537.108151/94‑1 (MQ=60)
                                                                             TCGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTA                  <  SRR4302537.2688776/94‑1 (MQ=60)
                                                                              CGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAC                 <  SRR4302537.422883/94‑1 (MQ=60)
                                                                              CGAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTAC                 <  SRR4302537.5868012/94‑1 (MQ=60)
                                                                               GAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACC                <  SRR4302537.2099331/94‑1 (MQ=60)
                                                                               GAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACC                <  SRR4302537.8174/94‑1 (MQ=60)
                                                                               GAACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACC                <  SRR4302537.2646110/94‑1 (MQ=60)
                                                                                AACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCG               >  SRR4302537.1556699/1‑94 (MQ=60)
                                                                                AACAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCG               >  SRR4302537.719674/1‑94 (MQ=60)
                                                                                  CAATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGA             <  SRR4302537.2475469/94‑1 (MQ=60)
                                                                                   AATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAA            >  SRR4302537.5397820/1‑94 (MQ=60)
                                                                                    ATATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAAC           >  SRR4302537.1379362/1‑94 (MQ=60)
                                                                                     TATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGACCCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACT          <  SRR4302537.5814464/94‑1 (MQ=60)
                                                                                      ATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTC         <  SRR4302537.5863209/94‑1 (MQ=60)
                                                                                      ATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTC         >  SRR4302537.669544/1‑94 (MQ=60)
                                                                                      ATACGGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTC         <  SRR4302537.643851/94‑1 (MQ=60)
                                                                                          GGGCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCAC     >  SRR4302537.2405496/1‑94 (MQ=60)
                                                                                            GCCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACAC   <  SRR4302537.5569403/94‑1 (MQ=60)
                                                                                             CCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACC  <  SRR4302537.5740046/94‑1 (MQ=60)
                                                                                             CCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACC  >  SRR4302537.4667536/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCAAGGTTGCCAGTAAAGTTGCTCAAACCGTTACGCGCCGGTTGCGGAACATAATCACGCCAGGCGACAGCGACCGGTCGAACAATATACGGGTCTAATACATTGAAGTTGAAGTTGTACATGGTGCGGTTGAACCCTTCTAACGGGTCAGAACGCCCTTGCTGATCTGTACCGGAACTCGCACACC  >  NC_000913/2462792‑2462978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑