Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,539,912	A→G	R207R (CGT→CGC)	cysU ←	sulfate, thiosulfate transport system permease T protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,539,912	0	A	G	99.2%	903.6 / ‑3.9	265	R207R (CGT→CGC)	cysU	sulfate, thiosulfate transport system permease T protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (124/139); total (125/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 272 total aligned reads displayed.

GTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCA  >  NC_000913/2539821‑2540002

gTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCg                                                                                          >  1:1934280/1‑94 (MQ=255)

  aaGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCggg                                                                                        >  2:5621360/1‑94 (MQ=255)

  aaGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCggg                                                                                        <  2:9716254/94‑1 (MQ=255)

    gcgcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTa                                                                                      <  1:948407/94‑1 (MQ=255)

    gcgcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGAT‑AAAATCACCGCGCCAAATTCACCAAGACTGCGGGTaa                                                                                     <  1:3285799/94‑1 (MQ=255)

     cgcACAAAAATCATCAGCGACGTCTCTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTaa                                                                                     <  2:8524814/94‑1 (MQ=255)

     cgcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTaa                                                                                     <  1:6707729/94‑1 (MQ=255)

      gcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTaaa                                                                                    <  2:9600849/94‑1 (MQ=255)

      gcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTaaa                                                                                    <  2:2719684/94‑1 (MQ=255)

       cacaAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAc                                                                                   <  1:2272542/94‑1 (MQ=255)

       cacaAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAc                                                                                   <  1:5769527/94‑1 (MQ=255)

       cacaAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAc                                                                                   <  2:2660333/94‑1 (MQ=255)

        ccaAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACg                                                                                  <  2:166531/93‑1 (MQ=255)

        acaAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACg                                                                                  <  1:9782031/94‑1 (MQ=255)

          aaaaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGAc                                                                                <  1:12075/94‑1 (MQ=255)

          aaaaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGAc                                                                                <  1:8683759/94‑1 (MQ=255)

           aaaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACa                                                                               <  2:3807343/94‑1 (MQ=255)

           aaaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACa                                                                               <  1:6893432/94‑1 (MQ=255)

           aaaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAAGCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACa                                                                               <  1:8450123/94‑1 (MQ=255)

              atcatcAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAgcg                                                                            >  2:629726/1‑94 (MQ=255)

                gctcaGCGACGTCACTTCCGTCTTCCACGCGATATTGCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGcc                                                                          <  2:6140133/92‑1 (MQ=255)

                 atcaGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCa                                                                         <  2:393466/94‑1 (MQ=255)

                   caGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcg                                                                       <  2:4652774/94‑1 (MQ=255)

                   caGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcg                                                                       <  2:5885929/94‑1 (MQ=255)

                    aGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcgc                                                                      >  2:8432912/1‑94 (MQ=255)

                       gACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcgcccg                                                                   <  2:4308617/94‑1 (MQ=255)

                         cGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcgcccgcc                                                                 <  2:2111387/94‑1 (MQ=255)

                          gTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGTCAGCGCCACGCCCGcca                                                                <  1:10179810/94‑1 (MQ=255)

                            cACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGccacc                                                              <  2:10366726/94‑1 (MQ=255)

                               ttCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgc                                                           >  2:9517082/1‑94 (MQ=255)

                               ttCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgc                                                           >  1:10225267/1‑94 (MQ=255)

                                tCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgcg                                                          <  2:9095839/94‑1 (MQ=255)

                                tCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgcg                                                          >  2:1170921/1‑94 (MQ=255)

                                 ccGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACACCCGCCACCAgcgc                                                         >  1:1666835/1‑94 (MQ=255)

                                  cGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGcc                                                        >  2:299599/1‑94 (MQ=255)

                                  cGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGcc                                                        <  2:8793440/94‑1 (MQ=255)

                                   gTCTTCCACGCGATATAACCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCg                                                       <  2:9417478/94‑1 (MQ=255)

                                   ctCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCg                                                       <  1:3778456/93‑1 (MQ=255)

                                     cTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGga                                                     >  2:6330675/1‑94 (MQ=255)

                                     cTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGga                                                     >  1:5278292/1‑94 (MQ=255)

                                       tCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGgaga                                                   >  2:7812210/1‑94 (MQ=255)

                                        ccACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGaa                                                  >  1:10302033/1‑94 (MQ=255)

                                          aCGCTATATTTCCGGCGATAAAAGTCCCCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAg                                                <  2:4500794/94‑1 (MQ=255)

                                          aCGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAg                                                >  1:1351671/1‑94 (MQ=255)

                                          aCGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAg                                                <  1:1798101/94‑1 (MQ=255)

                                            tcgATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCt                                              <  1:10510574/93‑1 (MQ=255)

                                             cgATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTc                                             >  2:9229553/1‑94 (MQ=255)

                                              gATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTcc                                            <  1:2974695/94‑1 (MQ=255)

                                              gATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTcc                                            >  1:4870428/1‑94 (MQ=255)

                                               atatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCg                                           <  1:3926299/94‑1 (MQ=255)

                                               atatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCg                                           <  2:8159802/94‑1 (MQ=255)

                                               atatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCg                                           <  1:7486306/94‑1 (MQ=255)

                                               atatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCg                                           <  2:1688667/94‑1 (MQ=255)

                                                   ttCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGgcag                                       <  1:6378720/94‑1 (MQ=255)

                                                     ccGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGgcagca                                     >  1:5395952/1‑94 (MQ=255)

                                                      cGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcac                                    <  2:6755258/94‑1 (MQ=255)

                                                      cGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcac                                    >  2:5740380/1‑94 (MQ=255)

                                                       ggCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcacc                                   >  1:2527970/1‑94 (MQ=255)

                                                        gCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcacca                                  >  2:7972708/1‑94 (MQ=255)

                                                        gCGATAAAAATCACCGCGCCAAATCCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcacca                                  <  1:1045195/94‑1 (MQ=255)

                                                         cGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcaccat                                 >  2:8943029/1‑93 (MQ=255)

                                                           aTAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACtt                               <  2:1485414/94‑1 (MQ=255)

                                                           aTAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACtt                               <  1:1722938/94‑1 (MQ=255)

                                                           aTAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACtt                               >  1:3744812/1‑94 (MQ=255)

                                                           aTAAAAAAAACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACtt                               <  2:2490949/94‑1 (MQ=255)

                                                            tAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACttt                              >  2:5279134/1‑94 (MQ=255)

                                                            tAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACttt                              <  2:9894721/94‑1 (MQ=255)

                                                             aaaaaTCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTg                             <  2:5058277/94‑1 (MQ=255)

                                                              aaaaTCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGc                            >  1:446497/1‑94 (MQ=255)

                                                               aaaTCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCa                           >  2:5758231/1‑94 (MQ=255)

                                                                  tCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGaa                        >  1:7146658/1‑94 (MQ=255)

                                                                  tCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGaa                        <  1:6245601/94‑1 (MQ=255)

                                                                  tCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGaa                        <  1:6064917/94‑1 (MQ=255)

                                                                   ccccGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGaaa                       <  1:7703006/92‑1 (MQ=255)

                                                                    cccGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAAc                      <  2:5173021/93‑1 (MQ=255)

                                                                      cgcgCCAAATTCACCAAGACTGCGGGGAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAActc                    >  2:8266696/1‑94 (MQ=255)

                                                                       gcgcCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAActct                   <  1:2713833/94‑1 (MQ=255)

                                                                         gcCAAATTCACCACGGCTGCGGGTAAACGACAGCGCCACCCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGc                 <  2:7008255/94‑1 (MQ=255)

                                                                         gcCAAATTCACCAAGACTGCGGGCAAACGACAGCGCCACGCCCTCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGc                 <  1:7930213/94‑1 (MQ=255)

                                                                          ccAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGcc                >  2:4068853/1‑94 (MQ=255)

                                                                          ccAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGcc                >  1:9215836/1‑94 (MQ=255)

                                                                           cAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCa               <  1:7630444/94‑1 (MQ=255)

                                                                            aaaTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAg              <  2:3598385/94‑1 (MQ=255)

                                                                            aaaTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAg              >  2:4807599/1‑94 (MQ=255)

                                                                             aaTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAgc             >  1:7325867/1‑94 (MQ=255)

                                                                             aaTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAgc             <  1:232819/94‑1 (MQ=255)

                                                                              aTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAgcg            >  1:9929279/1‑94 (MQ=255)

                                                                                tCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGcgcg          <  1:824459/94‑1 (MQ=255)

                                                                                tCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGcgcg          <  1:4561692/94‑1 (MQ=255)

                                                                                tCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGcgcg          >  2:3127537/1‑94 (MQ=255)

                                                                                 ccccAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGt         <  1:75359/92‑1 (MQ=255)

                                                                                  cccAAGACTGCGGTTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGtt        <  1:507268/93‑1 (MQ=255)

                                                                                  aCCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGtt        <  1:8511126/94‑1 (MQ=255)

                                                                                  aCCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGtt        <  2:4935056/94‑1 (MQ=255)

                                                                                  aCCAAGACTGCGGGTAAACGACAGCGCCACGACCGCCACCAGCGCCGGAGAAAGCTCCGGAAGCACCACTTTGCAGAAACTCTGCCAGCGCGtt        >  2:7409089/1‑94 (MQ=255)

                                                                                    cAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGc      <  1:9643939/94‑1 (MQ=255)

                                                                                     aaGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCa     >  1:6918051/1‑94 (MQ=255)

                                                                                     aaGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCa     <  2:1449455/94‑1 (MQ=255)

                                                                                        aCTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCa  >  2:2296435/1‑94 (MQ=255)

                                                                                        aCTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCa  <  2:8676317/94‑1 (MQ=255)

GTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCA  >  NC_000913/2539821‑2540002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGC  >  NC_000913/2539819‑2540005

CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTG                                                                                               <  SRR4302545.8495864/94‑1 (MQ=60)

CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTG                                                                                               <  SRR4302545.628097/94‑1 (MQ=60)

CTGTAAGCGCACAAAAATCATCAGCGACGTCACTGCCGTCTTCCACGCGATATTTCCGTCAATAAAAAGCCCCGCGCCAAATTCACCAAGACTG                                                                                               <  SRR4302545.1064936/94‑1 (MQ=60)

 TGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGC                                                                                              >  SRR4302545.2000120/1‑94 (MQ=60)

 TGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGC                                                                                              >  SRR4302545.1101678/1‑94 (MQ=60)

 TGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGC                                                                                              <  SRR4302545.4263310/94‑1 (MQ=60)

  GTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCG                                                                                             >  SRR4302545.1349105/1‑94 (MQ=60)

  GTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCG                                                                                             >  SRR4302545.1982212/1‑94 (MQ=60)

  GTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCG                                                                                             >  SRR4302545.7730998/1‑94 (MQ=60)

   TAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATCGAAATCACCGCGCCAAGTTCACCAAGACTGCGG                                                                                            >  SRR4302545.5278048/1‑94 (MQ=60)

   TAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGG                                                                                            <  SRR4302545.6600070/94‑1 (MQ=60)

   TAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGG                                                                                            >  SRR4302545.2358081/1‑94 (MQ=60)

      gcgcgcaaaattcatcggccacgtcgctgaCGTCTTCCACGCGATATTTCCGGAGATAAAAAGCACCGCGTCAAATTCCCCAAGACCGCGGGTA                                                                                         <  SRR4302545.7228678/64‑1 (MQ=37)

      GCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTA                                                                                         >  SRR4302545.10569729/1‑94 (MQ=60)

      GCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTA                                                                                         <  SRR4302545.969694/94‑1 (MQ=60)

      GCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGAT‑AAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAA                                                                                        <  SRR4302545.3366276/94‑1 (MQ=60)

       CGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAA                                                                                        <  SRR4302545.6891647/94‑1 (MQ=60)

        GCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAA                                                                                       <  SRR4302545.7090152/94‑1 (MQ=60)

         CACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAC                                                                                      <  SRR4302545.5924573/94‑1 (MQ=60)

         CACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAC                                                                                      <  SRR4302545.8029548/94‑1 (MQ=60)

         CACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAC                                                                                      <  SRR4302545.8627382/94‑1 (MQ=60)

         CACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAC                                                                                      <  SRR4302545.2327806/94‑1 (MQ=60)

          ACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACG                                                                                     <  SRR4302545.10072855/94‑1 (MQ=60)

            AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGAC                                                                                   <  SRR4302545.12373/94‑1 (MQ=60)

            AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGAC                                                                                   <  SRR4302545.8935650/94‑1 (MQ=60)

             AAAGTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACA                                                                                  <  SRR4302545.8694410/94‑1 (MQ=60)

             AAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGTCTGCGGGTAAACGACA                                                                                  <  SRR4302545.7403381/94‑1 (MQ=60)

             AAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACA                                                                                  <  SRR4302545.7083101/94‑1 (MQ=60)

             AAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACA                                                                                  >  SRR4302545.1951053/1‑94 (MQ=60)

             AAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAAGCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACA                                                                                  <  SRR4302545.8694409/94‑1 (MQ=60)

              AAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAG                                                                                 <  SRR4302545.5826370/94‑1 (MQ=60)

               AATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGC                                                                                <  SRR4302545.7831210/94‑1 (MQ=60)

                ATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCG                                                                               >  SRR4302545.9625830/1‑94 (MQ=60)

                 TCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGC                                                                              <  SRR4302545.3878931/94‑1 (MQ=60)

                  CATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCC                                                                             <  SRR4302545.1700683/94‑1 (MQ=60)

                   ATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCA                                                                            >  SRR4302545.2593400/1‑94 (MQ=60)

                     CAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACG                                                                          <  SRR4302545.3865569/94‑1 (MQ=60)

                      AGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGC                                                                         >  SRR4302545.1737824/1‑94 (MQ=60)

                        CGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCCCCAAGACTGCGGGTAAACGACAGCGCCACGCCC                                                                       <  SRR4302545.3227171/94‑1 (MQ=60)

                        CGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCC                                                                       <  SRR4302545.3661209/94‑1 (MQ=60)

                        CGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCC                                                                       >  SRR4302545.5508730/1‑94 (MQ=60)

                         GACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCG                                                                      <  SRR4302545.7584019/94‑1 (MQ=60)

                         GACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCG                                                                      <  SRR4302545.6240265/94‑1 (MQ=60)

                          ACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGC                                                                     >  SRR4302545.6988572/1‑94 (MQ=60)

                          ACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGC                                                                     >  SRR4302545.6988573/1‑94 (MQ=60)

                           CGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCC                                                                    <  SRR4302545.7795803/94‑1 (MQ=60)

                            GTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGTCAGCGCCACGCCCGCCA                                                                   <  SRR4302545.10480675/94‑1 (MQ=60)

                              CACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACC                                                                 >  SRR4302545.2474023/1‑94 (MQ=60)

                               ACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCA                                                                >  SRR4302545.601290/1‑94 (MQ=60)

                               ACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCA                                                                <  SRR4302545.8742911/94‑1 (MQ=60)

                                CTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAG                                                               >  SRR4302545.1212987/1‑94 (MQ=60)

                                 TTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGC                                                              >  SRR4302545.10527292/1‑94 (MQ=60)

                                  TCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGCCTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCG                                                             <  SRR4302545.8506661/94‑1 (MQ=60)

                                  TCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCG                                                             >  SRR4302545.6463938/1‑94 (MQ=60)

                                   CCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACACCCGCCACCAGCGC                                                            >  SRR4302545.1706248/1‑94 (MQ=60)

                                    CGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCC                                                           <  SRR4302545.9854170/94‑1 (MQ=60)

                                    CGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCC                                                           >  SRR4302545.3467740/1‑94 (MQ=60)

                                    CGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCC                                                           >  SRR4302545.10705695/1‑94 (MQ=60)

                                    CGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCC                                                           >  SRR4302545.11071/1‑94 (MQ=60)

                                     CTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCG                                                          <  SRR4302545.3871871/94‑1 (MQ=60)

                                       CTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGA                                                        <  SRR4302545.528604/94‑1 (MQ=60)

                                       CTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGA                                                        >  SRR4302545.5414857/1‑94 (MQ=60)

                                          CCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAA                                                     <  SRR4302545.6101321/94‑1 (MQ=60)

                                          CCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAA                                                     >  SRR4302545.10605851/1‑94 (MQ=60)

                                          CCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAA                                                     >  SRR4302545.2756704/1‑94 (MQ=60)

                                           CACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAA                                                    >  SRR4302545.4962236/1‑94 (MQ=60)

                                            CCGCGATATTTCCGGCGATAAAAATCCCCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAG                                                   <  SRR4302545.7689985/94‑1 (MQ=60)

                                            ACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAATCGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAG                                                   <  SRR4302545.10750364/94‑1 (MQ=60)

                                            ACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAG                                                   >  SRR4302545.1381886/1‑94 (MQ=60)

                                            ACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAG                                                   <  SRR4302545.8782171/94‑1 (MQ=60)

                                            ACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAG                                                   <  SRR4302545.1841554/94‑1 (MQ=60)

                                            ACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAG                                                   >  SRR4302545.528544/1‑94 (MQ=60)

                                            ACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAG                                                   <  SRR4302545.1841555/94‑1 (MQ=60)

                                             CGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGC                                                  >  SRR4302545.1295401/1‑94 (MQ=60)

                                              TCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCT                                                 <  SRR4302545.10820321/94‑1 (MQ=60)

                                               CGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTC                                                <  SRR4302545.1976787/94‑1 (MQ=60)

                                               CGATATTTCCGGCGATAAAAATCACAGCGCCAAAATCACCAAGACTACGTGTAAACGACAGCGCCACGCCCGCCACCAGCGCTGGAGAAAGCTC                                                >  SRR4302545.6464292/1‑94 (MQ=60)

                                                GATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCC                                               <  SRR4302545.3047178/94‑1 (MQ=60)

                                                GATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCC                                               >  SRR4302545.4994301/1‑94 (MQ=60)

                                                 ATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCG                                              >  SRR4302545.10556487/1‑94 (MQ=60)

                                                 ATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCG                                              <  SRR4302545.4024307/94‑1 (MQ=60)

                                                 ATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCG                                              <  SRR4302545.7494140/94‑1 (MQ=60)

                                                 ATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCG                                              >  SRR4302545.4609929/1‑94 (MQ=60)

                                                 ATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCG                                              <  SRR4302545.5532525/94‑1 (MQ=60)

                                                 ATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCG                                              <  SRR4302545.7698183/94‑1 (MQ=60)

                                                  TATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGG                                             <  SRR4302545.2400120/94‑1 (MQ=60)

                                                    TTTCCGGCGATAAAAATCACCGCGCCAACTTCACCAAGCCTGCGGCTAACCGACAGCGCCCCGCCCGCCACCAGCGCCGAAGAAAGCTCCGGCA                                           <  SRR4302545.6441915/94‑1 (MQ=60)

                                                     TTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAG                                          <  SRR4302545.6553112/94‑1 (MQ=60)

                                                     TTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAG                                          <  SRR4302545.6553113/94‑1 (MQ=60)

                                                      TCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGC                                         >  SRR4302545.10487279/1‑94 (MQ=60)

                                                       CCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCA                                        >  SRR4302545.5536549/1‑94 (MQ=60)

                                                        CGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCAC                                       <  SRR4302545.8293870/94‑1 (MQ=60)

                                                         GGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACC                                      >  SRR4302545.2589054/1‑94 (MQ=60)

                                                          GCGATAAAAATCACCGCGCCAAATCCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCA                                     <  SRR4302545.1067985/94‑1 (MQ=60)

                                                            GATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACT                                   >  SRR4302545.2346096/1‑94 (MQ=60)

                                                             ATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTT                                  >  SRR4302545.3837001/1‑94 (MQ=60)

                                                             ATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTT                                  <  SRR4302545.1763979/94‑1 (MQ=60)

                                                                AAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGC                               >  SRR4302545.456403/1‑94 (MQ=60)

                                                                  AATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAG                             >  SRR4302545.9015663/1‑94 (MQ=60)

                                                                  AATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAG                             <  SRR4302545.7089356/94‑1 (MQ=60)

                                                                   agccCCGCGCCAACTTCACCAAGTCTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGA                            <  SRR4302545.826191/90‑1 (MQ=60)

                                                                   ATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGTCAGCACCACTTTGCAGA                            >  SRR4302545.8149037/1‑94 (MQ=60)

                                                                   ATCACAGCGCCAAATTCAACAAGACTGCGGGTAAACGATAGCGCCACAGCAGCCACCAGCGCCGGAGAAAGCTCCCGCAGCACCCCGTTGCAGA                            >  SRR4302545.983834/1‑94 (MQ=42)

                                                                    TCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAA                           <  SRR4302545.6415829/94‑1 (MQ=60)

                                                                    TCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAA                           <  SRR4302545.6229521/94‑1 (MQ=60)

                                                                    TCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAA                           >  SRR4302545.7345001/1‑94 (MQ=60)

                                                                     CCCCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAA                          <  SRR4302545.7921080/94‑1 (MQ=60)

                                                                     CACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAA                          >  SRR4302545.5201635/1‑94 (MQ=60)

                                                                     CACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAA                          <  SRR4302545.1410900/94‑1 (MQ=60)

                                                                     CACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAA                          >  SRR4302545.5201636/1‑94 (MQ=60)

                                                                      CCCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAAC                         <  SRR4302545.3953379/94‑1 (MQ=60)

                                                                      ACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAAC                         <  SRR4302545.4434127/94‑1 (MQ=60)

                                                                        CGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTC                       >  SRR4302545.4586662/1‑94 (MQ=60)

                                                                         GCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCT                      <  SRR4302545.2779251/94‑1 (MQ=60)

                                                                         GCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCT                      <  SRR4302545.6274510/94‑1 (MQ=60)

                                                                           GCCAAATTCACCAAGACTGCGGGCAAACGACAGCGCCACGCCCTCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGC                    <  SRR4302545.8155444/94‑1 (MQ=60)

                                                                            CCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCC                   >  SRR4302545.10176292/1‑94 (MQ=60)

                                                                            CCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCC                   >  SRR4302545.9485999/1‑94 (MQ=60)

                                                                            CCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCC                   <  SRR4302545.2239244/94‑1 (MQ=60)

                                                                             CAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCA                  <  SRR4302545.7846371/94‑1 (MQ=60)

                                                                             CAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCA                  >  SRR4302545.7879617/1‑94 (MQ=60)

                                                                              AAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAG                 >  SRR4302545.10040464/1‑94 (MQ=60)

                                                                               AATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGC                >  SRR4302545.7531385/1‑94 (MQ=60)

                                                                               AATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGC                <  SRR4302545.237641/94‑1 (MQ=60)

                                                                                ATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCG               >  SRR4302545.10224148/1‑94 (MQ=60)

                                                                                  TCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCG             <  SRR4302545.4678717/94‑1 (MQ=60)

                                                                                  TCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCG             <  SRR4302545.3107437/94‑1 (MQ=60)

                                                                                  TCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCG             <  SRR4302545.842948/94‑1 (MQ=60)

                                                                                   CCCCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGT            <  SRR4302545.77047/94‑1 (MQ=60)

                                                                                   CACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGT            >  SRR4302545.2821932/1‑94 (MQ=60)

                                                                                   CACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGT            >  SRR4302545.1799995/1‑94 (MQ=60)

                                                                                    CCCAAGACTGCGGTTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTT           <  SRR4302545.518646/94‑1 (MQ=60)

                                                                                    ACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTT           <  SRR4302545.8757027/94‑1 (MQ=60)

                                                                                     CCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTG          >  SRR4302545.961907/1‑94 (MQ=60)

                                                                                      CAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGC         >  SRR4302545.10074106/1‑94 (MQ=60)

                                                                                      CAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGC         <  SRR4302545.9928602/94‑1 (MQ=60)

                                                                                      CAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGC         >  SRR4302545.7851233/1‑94 (MQ=60)

                                                                                       AAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCA        <  SRR4302545.1073930/94‑1 (MQ=60)

                                                                                       AAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCA        >  SRR4302545.7108656/1‑94 (MQ=60)

                                                                                       AAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCA        >  SRR4302545.9860806/1‑94 (MQ=60)

                                                                                       AAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCA        >  SRR4302545.7309844/1‑94 (MQ=60)

                                                                                        AGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCAC       >  SRR4302545.4064016/1‑94 (MQ=60)

                                                                                         GACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACC      >  SRR4302545.9160017/1‑94 (MQ=60)

                                                                                            TGCGGTTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAG   <  SRR4302545.2506130/94‑1 (MQ=60)

                                                                                             GCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGCAACTCTGCCAGCGCGTTGCACCAAGC  >  SRR4302545.10477554/1‑94 (MQ=60)

                                                                                             GCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGC  <  SRR4302545.10834699/94‑1 (MQ=60)

                                                                                             GCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGC  >  SRR4302545.3401097/1‑94 (MQ=60)

                                                                                             GCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGC  <  SRR4302545.5383914/94‑1 (MQ=60)

CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGC  >  NC_000913/2539819‑2540005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`