Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,539,912 A→G R207R (CGT→CGC)  cysU ← sulfate, thiosulfate transport system permease T protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,539,9120AG100.0% 663.5 / NA 189R207R (CGT→CGC) cysUsulfate, thiosulfate transport system permease T protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (113/76);  total (113/76)

Only 100 of 196 total aligned reads displayed.

AAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCAC  >  NC_000913/2539823‑2540000
                                                                                         |                                                                                        
aaGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCgg                                                                                       >  1:5163893/1‑93 (MQ=255)
  gcgcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGt                                                                                     <  2:5390426/93‑1 (MQ=255)
  gcgcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGt                                                                                     <  1:2028472/93‑1 (MQ=255)
  gcgcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGt                                                                                     >  2:2222350/1‑93 (MQ=255)
     cacaAAAATCATCAGCGACGGCACTTCCGTCTTCCACGCGATATTTCCGGCTATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTaaa                                                                                  <  2:4322032/93‑1 (MQ=255)
      acaAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAc                                                                                 <  1:1381415/93‑1 (MQ=255)
      acaAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAAc                                                                                 <  2:1911041/93‑1 (MQ=255)
        aaaaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGa                                                                               >  1:4258937/1‑93 (MQ=255)
        aaaaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGa                                                                               >  2:2059835/1‑93 (MQ=255)
         aaaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGAc                                                                              >  2:3141878/1‑93 (MQ=255)
          aaaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACa                                                                             >  1:5471737/1‑93 (MQ=255)
           aaTCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAg                                                                            >  1:5436316/1‑93 (MQ=255)
             tcatcaGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAgcg                                                                          >  2:5553595/1‑93 (MQ=255)
                tcaGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCa                                                                       >  2:350063/1‑93 (MQ=255)
                tcaGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCa                                                                       >  2:4965942/1‑93 (MQ=255)
                  aGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcg                                                                     >  2:3446259/1‑93 (MQ=255)
                   gCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcgc                                                                    >  2:3602405/1‑93 (MQ=255)
                   gCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcgc                                                                    >  2:4114499/1‑93 (MQ=255)
                      aCGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcgcccg                                                                 >  2:2472765/1‑93 (MQ=255)
                      aCGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcgcccg                                                                 >  1:1153109/1‑93 (MQ=255)
                       cGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCTCCAcgcccgc                                                                >  2:4683524/1‑93 (MQ=255)
                       cGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAcgcccgc                                                                >  2:5810115/1‑93 (MQ=255)
                          cACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGccac                                                             <  2:1134860/93‑1 (MQ=255)
                           aCTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGccacc                                                            >  2:881729/1‑93 (MQ=255)
                            cTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGccacca                                                           <  2:1853443/93‑1 (MQ=255)
                             ttCCGTCTGCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATGCACCAAGACTGCGGGTAAGCGACAGCGCCACGCCCGCCACCAg                                                          <  1:5252680/93‑1 (MQ=255)
                              tCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgc                                                         >  1:1238269/1‑93 (MQ=255)
                              tCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgc                                                         <  1:3467345/93‑1 (MQ=255)
                              tCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgc                                                         <  1:5318054/93‑1 (MQ=255)
                              tCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgc                                                         >  2:2890439/1‑93 (MQ=255)
                              tCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgc                                                         >  1:5867985/1‑93 (MQ=255)
                               ccGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgcg                                                        >  1:5035715/1‑93 (MQ=255)
                               ccGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAgcg                                                        <  1:3881737/93‑1 (MQ=255)
                                 gTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGcc                                                      >  1:3563654/1‑93 (MQ=255)
                                  tCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCg                                                     >  2:6061484/1‑93 (MQ=255)
                                    ttCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGga                                                   >  2:1958330/1‑93 (MQ=255)
                                     tCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGgag                                                  >  2:4507145/1‑93 (MQ=255)
                                       cACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGaa                                                >  2:646591/1‑93 (MQ=255)
                                       cACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGaa                                                >  1:848277/1‑93 (MQ=255)
                                        aCGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGaaa                                               >  2:5180655/1‑93 (MQ=255)
                                        aCGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGaaa                                               >  1:4274937/1‑93 (MQ=255)
                                          gcgATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGc                                             <  2:3311842/93‑1 (MQ=255)
                                           cgATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCt                                            >  1:4508247/1‑93 (MQ=255)
                                            gATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTc                                           >  1:280245/1‑93 (MQ=255)
                                            gATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTc                                           >  2:3843325/1‑93 (MQ=255)
                                            gATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTc                                           >  1:4901574/1‑93 (MQ=255)
                                             atatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTcc                                          >  1:5899206/1‑93 (MQ=255)
                                             atatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTcc                                          >  2:281863/1‑93 (MQ=255)
                                             atatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTcc                                          >  1:779436/1‑93 (MQ=255)
                                             atatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTcc                                          >  2:2003850/1‑93 (MQ=255)
                                              tatTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCg                                         <  2:2137238/93‑1 (MQ=255)
                                              tatTTCCGGAGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCg                                         >  1:4041454/1‑93 (MQ=255)
                                               atTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCgg                                        >  1:6329250/1‑93 (MQ=255)
                                                tttCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGgc                                       <  2:4019530/93‑1 (MQ=255)
                                                tttCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGgc                                       <  2:367691/93‑1 (MQ=255)
                                                 ttCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGgca                                      >  2:4893273/1‑93 (MQ=255)
                                                  tCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGgcag                                     >  2:1622621/1‑93 (MQ=255)
                                                     ggCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcac                                  >  1:4736769/1‑93 (MQ=255)
                                                     ggCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcac                                  >  1:1534873/1‑93 (MQ=255)
                                                       cGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGGGCCACCCCCGCCACCAGCACCGGAGAAAGCTCCGGCGGcacca                                >  2:3737297/1‑93 (MQ=255)
                                                       cGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcacca                                >  2:2121541/1‑93 (MQ=255)
                                                       cGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcacca                                <  1:351616/93‑1 (MQ=255)
                                                        gATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcaccac                               >  1:3723364/1‑93 (MQ=255)
                                                        gATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcaccac                               >  1:3353923/1‑93 (MQ=255)
                                                        gATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcaccac                               >  2:4206668/1‑93 (MQ=255)
                                                          tAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCTGAGAAAGCTCCGACATCACCACtt                             >  1:3157129/1‑93 (MQ=255)
                                                          tAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACtt                             <  2:5013745/93‑1 (MQ=255)
                                                          tAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACtt                             >  2:1053237/1‑93 (MQ=255)
                                                           aaaaaTCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACttt                            <  2:3865270/93‑1 (MQ=255)
                                                           aaaaaTCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACttt                            <  1:2060169/93‑1 (MQ=255)
                                                            aaaaTCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTg                           >  2:4765419/1‑93 (MQ=255)
                                                             aaaTCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGTAAGCTCCGGCAGCACCACTTTGc                          >  2:1805204/1‑93 (MQ=255)
                                                             aaaTCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGc                          >  1:697997/1‑93 (MQ=255)
                                                                tCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGa                       <  1:6273057/93‑1 (MQ=255)
                                                                  cccGCGCCAAATTCACCAAGACTGCGTGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGaaa                     <  1:3124921/92‑1 (MQ=255)
                                                                   ccGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAAc                    >  1:5699783/1‑93 (MQ=255)
                                                                     gcgcCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAActc                  <  2:1156388/93‑1 (MQ=255)
                                                                     gcgcCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAActc                  <  1:6447837/93‑1 (MQ=255)
                                                                      cgcCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAActcc                 <  1:5775227/93‑2 (MQ=255)
                                                                       gcCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTg                >  1:993371/1‑93 (MQ=255)
                                                                       gcCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTg                <  1:2846073/93‑1 (MQ=255)
                                                                       gcCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTg                >  2:5081210/1‑93 (MQ=255)
                                                                        ccAAATCCACAAGGACTGCGCGTAAGCGACAGCGCCGCGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCGGAAACTCTGc               <  2:3547738/93‑1 (MQ=255)
                                                                         cAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGcc              <  2:5401185/93‑1 (MQ=255)
                                                                         cAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGcc              >  1:5424200/1‑93 (MQ=255)
                                                                         cAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGcc              >  2:2620519/1‑93 (MQ=255)
                                                                          aaaTTCACCAAGACTGCGGGTAACCGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCa             <  1:1876175/93‑1 (MQ=255)
                                                                           aaTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAg            >  2:1233612/1‑93 (MQ=255)
                                                                           aaTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAg            <  1:1643221/93‑1 (MQ=255)
                                                                            aTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAgc           >  1:4799748/1‑93 (MQ=255)
                                                                            aTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAgc           <  2:5999954/93‑1 (MQ=255)
                                                                            aTTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAgc           <  1:4176266/93‑1 (MQ=255)
                                                                             ttCACCAAGACTGCGGGTAAACGATAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAgcg          >  2:6270886/1‑93 (MQ=255)
                                                                                cccAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGt       <  1:1179591/92‑1 (MQ=255)
                                                                                aCCAAGACTGCGGGGAAACGACGGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGACAGCACCACTTTGCAGAAACTCTGCCAGCGCGt       <  1:3926516/93‑1 (MQ=255)
                                                                                 ccAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGtt      <  2:5325169/93‑1 (MQ=255)
                                                                                 ccAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGtt      <  1:3094354/93‑1 (MQ=255)
                                                                                  cAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTg     <  1:1733267/93‑1 (MQ=255)
                                                                                    aGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCa   >  1:2447682/1‑93 (MQ=255)
                                                                                     gACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCAc  <  2:1166356/93‑1 (MQ=255)
                                                                                         |                                                                                        
AAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCAC  >  NC_000913/2539823‑2540000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCA  >  NC_000913/2539819‑2540002
                                                                                             |                                                                                          
CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTG                                                                                            >  SRR4302537.3265225/1‑94 (MQ=60)
CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTG                                                                                            >  SRR4302537.4544730/1‑94 (MQ=60)
CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTG                                                                                            >  SRR4302537.5572101/1‑94 (MQ=60)
CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTG                                                                                            >  SRR4302537.3466570/1‑94 (MQ=60)
 TGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGC                                                                                           >  SRR4302537.1745981/1‑94 (MQ=60)
 TGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGC                                                                                           >  SRR4302537.1954544/1‑94 (MQ=60)
   TAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGG                                                                                         >  SRR4302537.5199479/1‑94 (MQ=60)
    AAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGG                                                                                        <  SRR4302537.4341710/94‑1 (MQ=60)
      GCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTA                                                                                      <  SRR4302537.2042614/94‑1 (MQ=60)
       CGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAA                                                                                     <  SRR4302537.2676548/94‑1 (MQ=60)
       CGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAA                                                                                     <  SRR4302537.5534982/94‑1 (MQ=60)
          ACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACG                                                                                  <  SRR4302537.1391053/94‑1 (MQ=60)
           CAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGA                                                                                 >  SRR4302537.4288535/1‑94 (MQ=60)
            AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGAC                                                                                <  SRR4302537.5832640/94‑1 (MQ=60)
            AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGAC                                                                                <  SRR4302537.653153/94‑1 (MQ=60)
             AAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACA                                                                               <  SRR4302537.1435031/94‑1 (MQ=60)
             AAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACA                                                                               >  SRR4302537.5509070/1‑94 (MQ=60)
              AAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAG                                                                              >  SRR4302537.5473396/1‑94 (MQ=60)
               AATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGC                                                                             <  SRR4302537.5773057/94‑1 (MQ=60)
               AATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGC                                                                             >  SRR4302537.3687888/1‑94 (MQ=60)
                ATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCG                                                                            >  SRR4302537.5211608/1‑94 (MQ=60)
                  CATCAGCGACGTCACTTCCGGCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCC                                                                          <  SRR4302537.5442531/94‑1 (MQ=60)
                    TCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAC                                                                        <  SRR4302537.3778944/94‑1 (MQ=60)
                    TCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCAC                                                                        <  SRR4302537.284789/94‑1 (MQ=60)
                     CAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACG                                                                       <  SRR4302537.888723/94‑1 (MQ=60)
                      AGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGC                                                                      >  SRR4302537.531906/1‑94 (MQ=60)
                       GCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCC                                                                     >  SRR4302537.4238736/1‑94 (MQ=60)
                        CGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCC                                                                    >  SRR4302537.6352335/1‑94 (MQ=60)
                         GACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCG                                                                   >  SRR4302537.5933791/1‑94 (MQ=60)
                         GACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCG                                                                   >  SRR4302537.1161133/1‑94 (MQ=60)
                           CGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCGCCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCC                                                                 >  SRR4302537.3478874/1‑94 (MQ=60)
                            GTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCA                                                                >  SRR4302537.3951528/1‑94 (MQ=60)
                            GTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCA                                                                >  SRR4302537.4840049/1‑94 (MQ=60)
                              CACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACC                                                              <  SRR4302537.1196546/94‑1 (MQ=60)
                               ACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCA                                                             >  SRR4302537.4787895/1‑94 (MQ=60)
                                CTTCCTCCTTCCACGCGATATTTCCGGCGATAAAAATCGCCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAG                                                            <  SRR4302537.4011783/94‑1 (MQ=60)
                                 TTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGC                                                           >  SRR4302537.5908241/1‑94 (MQ=60)
                                 TTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGC                                                           >  SRR4302537.1246882/1‑94 (MQ=60)
                                 TTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGC                                                           >  SRR4302537.350793/1‑94 (MQ=60)
                                 TTCCGTCTGCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATGCACCAAGACTGCGGGTAAGCGACAGCGCCACGCCCGCCACCAGC                                                           <  SRR4302537.5288823/94‑1 (MQ=60)
                                  TCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCG                                                          <  SRR4302537.5354565/94‑1 (MQ=60)
                                  TCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCG                                                          >  SRR4302537.5070380/1‑94 (MQ=60)
                                  TCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCG                                                          <  SRR4302537.3491869/94‑1 (MQ=60)
                                   CCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGC                                                         <  SRR4302537.3908891/94‑1 (MQ=60)
                                    CGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCC                                                        >  SRR4302537.3588787/1‑94 (MQ=60)
                                     GTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTAACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCG                                                       >  SRR4302537.1630771/1‑94 (MQ=60)
                                      TCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGG                                                      <  SRR4302537.1263198/94‑1 (MQ=60)
                                       CTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGA                                                     >  SRR4302537.1874074/1‑94 (MQ=60)
                                        TTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAG                                                    <  SRR4302537.550020/94‑1 (MQ=60)
                                          CCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAA                                                  >  SRR4302537.854087/1‑94 (MQ=60)
                                           CACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAA                                                 >  SRR4302537.4304647/1‑94 (MQ=60)
                                              GCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCT                                              >  SRR4302537.4539420/1‑94 (MQ=60)
                                               CGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTC                                             >  SRR4302537.282201/1‑94 (MQ=60)
                                               CGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTC                                             >  SRR4302537.4935407/1‑94 (MQ=60)
                                                GATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCC                                            >  SRR4302537.5939684/1‑94 (MQ=60)
                                                GATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCC                                            >  SRR4302537.784786/1‑94 (MQ=60)
                                                GATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCC                                            >  SRR4302537.2062051/1‑94 (MQ=60)
                                                 ATATTTCCGGAGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCG                                           >  SRR4302537.4069723/1‑94 (MQ=60)
                                                  TATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGG                                          >  SRR4302537.6372532/1‑94 (MQ=60)
                                                     TTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAG                                       <  SRR4302537.6179885/94‑1 (MQ=60)
                                                      TCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGC                                      <  SRR4302537.759300/94‑1 (MQ=60)
                                                       CCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCAACCAGCGCCGGAGAAAGCTCCGGCAGCA                                     >  SRR4302537.4196950/1‑94 (MQ=60)
                                                        CGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCAC                                    >  SRR4302537.4769513/1‑94 (MQ=60)
                                                        CGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCAC                                    >  SRR4302537.1545593/1‑94 (MQ=60)
                                                        CGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCAC                                    >  SRR4302537.2170142/1‑94 (MQ=60)
                                                          GCGATAAAAATCCCCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCA                                  <  SRR4302537.547029/94‑1 (MQ=60)
                                                           CGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCAC                                 >  SRR4302537.3377677/1‑94 (MQ=60)
                                                           CGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCAC                                 <  SRR4302537.354060/94‑1 (MQ=60)
                                                           CGATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCAC                                 >  SRR4302537.3749528/1‑94 (MQ=60)
                                                             ATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCTGAGAAAGCTCCGACATCACCACTT                               >  SRR4302537.3179291/1‑94 (MQ=60)
                                                             ATAAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTT                               >  SRR4302537.189940/1‑94 (MQ=60)
                                                               AAAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTG                             <  SRR4302537.2074631/94‑1 (MQ=60)
                                                                AAAATCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGC                            >  SRR4302537.702778/1‑94 (MQ=60)
                                                                    TCACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAA                        <  SRR4302537.6316103/94‑1 (MQ=60)
                                                                      CCCGCGCCAAATTCACCAAGACTGCGTGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAAC                      <  SRR4302537.3146804/94‑1 (MQ=60)
                                                                      ACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAAC                      <  SRR4302537.3146803/94‑1 (MQ=60)
                                                                      ACCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAAC                      >  SRR4302537.5738788/1‑94 (MQ=60)
                                                                       CCGCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACT                     <  SRR4302537.1682594/94‑1 (MQ=60)
                                                                         GCGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCT                   <  SRR4302537.6491570/94‑1 (MQ=60)
                                                                          CGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTG                  <  SRR4302537.3074033/94‑1 (MQ=60)
                                                                          CGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTG                  >  SRR4302537.1000234/1‑94 (MQ=60)
                                                                          CGCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCCG                  <  SRR4302537.5814812/94‑1 (MQ=60)
                                                                           GCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGC                 <  SRR4302537.2865997/94‑1 (MQ=60)
                                                                           GCCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGC                 <  SRR4302537.4615573/94‑1 (MQ=60)
                                                                            CCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCC                >  SRR4302537.5461169/1‑94 (MQ=60)
                                                                            CCAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCC                >  SRR4302537.3334633/1‑94 (MQ=60)
                                                                             CAAATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGACGCAACACTTTGCAGAAACTCTGCCA               >  SRR4302537.4945928/1‑94 (MQ=60)
                                                                              AAATTCACCAAGACTGCGGGTAACCGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAG              <  SRR4302537.1889227/94‑1 (MQ=60)
                                                                               AATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGC             <  SRR4302537.1654731/94‑1 (MQ=60)
                                                                               AATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGC             >  SRR4302537.4832863/1‑94 (MQ=60)
                                                                                ATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCG            <  SRR4302537.5076443/94‑1 (MQ=60)
                                                                                ATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCG            <  SRR4302537.6454181/94‑1 (MQ=60)
                                                                                ATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCG            <  SRR4302537.4205421/94‑1 (MQ=60)
                                                                                ATTCACCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCG            <  SRR4302537.3159889/94‑1 (MQ=60)
                                                                                    CCCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTT        <  SRR4302537.1187800/94‑1 (MQ=60)
                                                                                    ACCAAGACTGCGGGGAAACGACGGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGACAGCACCACTTTGCAGAAACTCTGCCAGCGCGTT        <  SRR4302537.3953948/94‑1 (MQ=60)
                                                                                     CCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTG       <  SRR4302537.3115939/94‑1 (MQ=60)
                                                                                      CAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGC      <  SRR4302537.1745359/94‑1 (MQ=60)
                                                                                      CAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGC      <  SRR4302537.807835/94‑1 (MQ=60)
                                                                                       AAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCA     >  SRR4302537.2464903/1‑94 (MQ=60)
                                                                                       AAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCA     >  SRR4302537.3585042/1‑94 (MQ=60)
                                                                                          ACTGCGGGCAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGCTGCACCA  >  SRR4302537.3843690/1‑94 (MQ=60)
                                                                                             |                                                                                          
CTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCA  >  NC_000913/2539819‑2540002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑