Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,547,949 T→C D206G (GAC→GGC)  b2431 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,547,9490TC98.7% 791.8 / ‑0.6 238D206G (GAC→GGC) b2431hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base C (108/127);  total (111/127)
Fisher's exact test for biased strand distribution p-value = 1.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 256 total aligned reads displayed.

CGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCC  >  NC_000913/2547858‑2548039
                                                                                           |                                                                                          
 ggCAGGCTCTGGCGTACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGATGTTTCCGGACGTTCGTCGCCGCCGa                                                                                         >  1:9357967/1‑94 (MQ=255)
 ggCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGa                                                                                         >  2:2619043/1‑94 (MQ=255)
  gCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAt                                                                                        <  1:10133995/94‑1 (MQ=255)
  gCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAt                                                                                        <  1:3849874/94‑1 (MQ=255)
   cAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtc                                                                                       >  1:8370276/1‑94 (MQ=255)
    cggCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCTGCCGAtct                                                                                      <  1:6854497/93‑1 (MQ=255)
    aGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtct                                                                                      >  2:2482879/1‑94 (MQ=255)
    aGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtct                                                                                      >  1:2159371/1‑94 (MQ=255)
    aGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtct                                                                                      >  1:2261989/1‑94 (MQ=255)
     ggCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtctc                                                                                     <  2:6267125/94‑1 (MQ=255)
      gCTCTGGCGAGCAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATctct                                                                                    <  1:2932504/94‑1 (MQ=255)
       ctctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCtt                                                                                   <  2:1251384/94‑1 (MQ=255)
       ctctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCtt                                                                                   <  1:4517028/94‑1 (MQ=255)
       ctctGGCGAACAATCTTAAGCCCTTTGCCATCTACTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCtt                                                                                   <  2:9417699/94‑1 (MQ=255)
        tctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTc                                                                                  <  1:8195360/94‑1 (MQ=255)
        tctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTc                                                                                  <  2:8136920/94‑1 (MQ=255)
         ctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCg                                                                                 <  1:4277044/94‑1 (MQ=255)
           ggCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGtt                                                                               <  1:8013316/94‑1 (MQ=255)
           ggCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGtt                                                                               <  2:6537972/94‑1 (MQ=255)
             cGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTgg                                                                             >  2:5547644/1‑94 (MQ=255)
               aaCAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGcc                                                                           >  1:35629/1‑94 (MQ=255)
                aCAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCct                                                                          >  2:8094087/1‑94 (MQ=255)
                  aaTCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCctct                                                                        >  2:368438/1‑94 (MQ=255)
                  aaTCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCctct                                                                        >  2:1827778/1‑94 (MQ=255)
                    tCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCttt                                                                      <  2:7670592/94‑1 (MQ=255)
                    tCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCttt                                                                      >  2:9001505/1‑94 (MQ=255)
                     cTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCGGTACGTTCGTCTCCGCCGATCTCTTCGTTGGCCTCTTTg                                                                     <  2:5262167/94‑1 (MQ=255)
                     cTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTg                                                                     <  2:9689786/94‑1 (MQ=255)
                         aGCCCTTTGCCATCTTCTTTCAGATCAACGAGGGGGAGG‑GAGAGGGTTTCCGACGGTTGTTGACACCGAACTTTTAATTGGGCTCTTTgggtgg                                                                >  2:6796773/1‑90 (MQ=255)
                          gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTgcg                                                                <  2:4053894/94‑1 (MQ=255)
                          gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTgcg                                                                <  2:245468/94‑1 (MQ=255)
                           cccTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGCTGGCCTCTTTGGTgcgc                                                               >  2:2647459/1‑94 (MQ=255)
                             cTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTTGTGCGccc                                                             <  1:3276934/94‑1 (MQ=255)
                                tGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGAt                                                          <  2:745227/94‑1 (MQ=255)
                                tGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGAt                                                          <  2:503004/94‑1 (MQ=255)
                                 gCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATc                                                         >  1:1096678/1‑94 (MQ=255)
                                 gCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATc                                                         <  2:434157/94‑1 (MQ=255)
                                 gCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATc                                                         >  1:5829887/1‑94 (MQ=255)
                                 gCCATCTTCTGTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATc                                                         <  1:2514570/94‑1 (MQ=255)
                                   cATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAc                                                       >  2:4172626/1‑94 (MQ=255)
                                     tcttctTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCa                                                     >  2:6518340/1‑94 (MQ=255)
                                      cttcttTCAGATCAACGCGGGTGAGGTTAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAt                                                    <  2:7086447/94‑1 (MQ=255)
                                      cttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAt                                                    <  2:1109036/94‑1 (MQ=255)
                                        tcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAtct                                                  <  1:6990362/94‑1 (MQ=255)
                                        tcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAtct                                                  >  2:2095571/1‑94 (MQ=255)
                                          tttCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTcc                                                <  1:6759604/94‑1 (MQ=255)
                                          tttCAAACCAACGCGGGTTAGGTGAGAGGTTTCCGGACGTTCGTCCCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTcc                                                <  1:3465779/94‑1 (MQ=255)
                                           ttCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCt                                               <  2:6018585/94‑1 (MQ=255)
                                           ttCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCt                                               >  1:10264453/1‑94 (MQ=255)
                                            tCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTg                                              <  1:5196071/94‑1 (MQ=255)
                                            tCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTg                                              <  2:5140792/94‑1 (MQ=255)
                                            tCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTg                                              >  1:8845435/1‑94 (MQ=255)
                                              aGATCAACGCGGGTGAGGGGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTTGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAt                                            <  2:3809946/94‑1 (MQ=255)
                                               gATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATc                                           >  2:906551/1‑94 (MQ=255)
                                                aTCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCg                                          <  2:1367533/94‑1 (MQ=255)
                                                aTCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCg                                          <  1:413786/94‑1 (MQ=255)
                                                aTCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCg                                          >  2:2785054/1‑94 (MQ=255)
                                                aTCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCg                                          >  1:8909255/1‑94 (MQ=255)
                                                 tCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGt                                         >  2:5385201/1‑94 (MQ=255)
                                                  cAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTg                                        >  2:8644037/1‑94 (MQ=255)
                                                   aaCGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGa                                       >  2:8834774/1‑94 (MQ=255)
                                                    aCGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGaa                                      >  1:9500619/1‑94 (MQ=255)
                                                    aCGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGaa                                      <  1:9850422/94‑1 (MQ=255)
                                                     cgcgGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAc                                     <  2:6758932/94‑1 (MQ=255)
                                                     cgcgGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAc                                     >  2:10119926/1‑94 (MQ=255)
                                                        gggTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGCACGCt                                  >  2:645037/1‑94 (MQ=255)
                                                        gggTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCt                                  <  1:6375637/94‑1 (MQ=255)
                                                        gggTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCt                                  >  2:101813/1‑94 (MQ=255)
                                                         ggtgaggtgaGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTc                                 <  1:9831574/94‑1 (MQ=255)
                                                          gtgaggtgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCa                                <  1:7357127/94‑1 (MQ=255)
                                                           tgaggtgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCAt                               <  1:8762230/94‑1 (MQ=255)
                                                             aggtgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATcc                             <  2:9023306/94‑1 (MQ=255)
                                                              ggtgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCg                            <  2:4501896/94‑1 (MQ=255)
                                                               gtgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCgg                           <  2:1036491/94‑1 (MQ=255)
                                                                tgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGt                          >  1:5331740/1‑94 (MQ=255)
                                                                 gagaGTTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGtt                         <  1:1989015/94‑1 (MQ=255)
                                                                 gagaGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGtt                         <  1:6177941/94‑1 (MQ=255)
                                                                  agagGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTg                        >  2:3186667/1‑94 (MQ=255)
                                                                    agGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCTCCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                      <  2:9817805/94‑1 (MQ=255)
                                                                    agGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                      <  1:7227668/94‑1 (MQ=255)
                                                                      gTTTCCGGATGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                    <  2:755045/94‑1 (MQ=255)
                                                                      gTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                    <  2:3082519/94‑1 (MQ=255)
                                                                      gTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                    >  1:5756/1‑94 (MQ=255)
                                                                      gTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                    <  1:9454944/94‑1 (MQ=255)
                                                                      gTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                    >  2:8578353/1‑94 (MQ=255)
                                                                       tttCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgctg                   >  2:7068088/1‑94 (MQ=255)
                                                                       tttCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgctg                   >  2:8378278/1‑94 (MQ=255)
                                                                        ttCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgctgc                  >  1:2697931/1‑94 (MQ=255)
                                                                           cGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTc               >  2:7185702/1‑94 (MQ=255)
                                                                           cGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTc               <  2:7918557/94‑1 (MQ=255)
                                                                            ggACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCa              <  2:5286906/94‑1 (MQ=255)
                                                                            ggACGTTCGTCGCCGCCGATCTCTTCCTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCa              <  2:10374068/94‑1 (MQ=255)
                                                                             gACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAg             >  1:10182454/1‑94 (MQ=255)
                                                                                 ttCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTtg         <  1:10084455/94‑1 (MQ=255)
                                                                                 tgcgtcgCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTtg         <  1:8338471/92‑1 (MQ=255)
                                                                                    gtcgCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTgtgt      >  1:1181254/1‑94 (MQ=255)
                                                                                      cgccgcCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTc    <  1:9164294/94‑1 (MQ=255)
                                                                                       gccgccGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTcc   <  1:1856443/94‑1 (MQ=255)
                                                                                        ccgccgATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTccc  <  2:5897359/94‑1 (MQ=255)
                                                                                        ccgccgATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTAGAGCTGCTTCAGGTTGTGTTccc  >  1:7552504/1‑94 (MQ=255)
                                                                                           |                                                                                          
CGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCC  >  NC_000913/2547858‑2548039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  >  NC_000913/2547856‑2548042
                                                                                             |                                                                                             
TACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGC                                                                                               >  SRR4302545.8416229/1‑94 (MQ=60)
TACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGGGAGGTGAGTGGTTTCCGGACGTTCGTCGCCGC                                                                                               >  SRR4302545.7397324/1‑94 (MQ=60)
 ACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCC                                                                                              <  SRR4302545.7396193/94‑1 (MQ=60)
 ACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCC                                                                                              <  SRR4302545.82905/94‑1 (MQ=60)
  CGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCG                                                                                             <  SRR4302545.7540389/94‑1 (MQ=60)
  CGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCG                                                                                             >  SRR4302545.2088819/1‑94 (MQ=60)
   GGCAGGCTCTGGCGTACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGATGTTTCCGGACGTTCGTCGCCGCCGA                                                                                            >  SRR4302545.9632447/1‑94 (MQ=60)
   GGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGA                                                                                            <  SRR4302545.6394490/94‑1 (MQ=60)
    GCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAT                                                                                           <  SRR4302545.10433683/94‑1 (MQ=60)
    GCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAT                                                                                           <  SRR4302545.3945957/94‑1 (MQ=60)
     CAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATC                                                                                          >  SRR4302545.8612676/1‑94 (MQ=60)
      CGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCTGCCGATCT                                                                                         <  SRR4302545.7042805/94‑1 (MQ=60)
      AGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGCGGATTCCGGACGTTCGTCGCCGCCGATCC                                                                                         >  SRR4302545.1125298/1‑94 (MQ=60)
      AGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCT                                                                                         >  SRR4302545.6947697/1‑94 (MQ=60)
      AGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCT                                                                                         >  SRR4302545.2317020/1‑94 (MQ=60)
      AGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCT                                                                                         >  SRR4302545.2212171/1‑94 (MQ=60)
       GGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTC                                                                                        >  SRR4302545.222319/1‑94 (MQ=60)
        GCTCTGGCGAGCAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCT                                                                                       <  SRR4302545.3003798/94‑1 (MQ=60)
        GCTCTGGCGAACACTCTTCAGCCCTTTGCCATCTTCTTACAGATCAACGCGGGTGACGTGAGAGGTTTCCGGACGTTCGTCCCCGTCGATCTCT                                                                                       <  SRR4302545.4929275/94‑1 (MQ=60)
         CTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTT                                                                                      <  SRR4302545.4632686/94‑1 (MQ=60)
          TCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTC                                                                                     <  SRR4302545.8432544/94‑1 (MQ=60)
          CCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCCCCGCCGATCTCTTC                                                                                     <  SRR4302545.5596528/94‑1 (MQ=60)
           CTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCG                                                                                    <  SRR4302545.4384497/94‑1 (MQ=60)
           CTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGGTTCCGGACGTTCGTCGCCGCCGATCTCTTCG                                                                                    >  SRR4302545.4354483/1‑94 (MQ=60)
             GGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTT                                                                                  <  SRR4302545.651358/94‑1 (MQ=60)
             GGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTT                                                                                  <  SRR4302545.8241438/94‑1 (MQ=60)
              GCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTG                                                                                 <  SRR4302545.8315130/94‑1 (MQ=60)
              GCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTG                                                                                 <  SRR4302545.7016429/94‑1 (MQ=60)
              GCGAACAATCTTAAGCCCTTTGCCATCTTCTGTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTG                                                                                 <  SRR4302545.6398281/94‑1 (MQ=60)
               CGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGG                                                                                <  SRR4302545.1648049/94‑1 (MQ=60)
               CGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGG                                                                                >  SRR4302545.1509627/1‑94 (MQ=60)
                 AACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCC                                                                              >  SRR4302545.36443/1‑94 (MQ=60)
                  ACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCT                                                                             >  SRR4302545.8458507/1‑94 (MQ=60)
                     atctgtagctCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGTTCTCTTCGTTGGCCTCTT                                                                          <  SRR4302545.10297272/84‑1 (MQ=60)
                     ATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTT                                                                          <  SRR4302545.10393871/94‑1 (MQ=60)
                         TCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGGTTCCGGACGTTCGTCGCCGCCGCTCTCTTCGTTGGCCTCTTTGGG                                                                      >  SRR4302545.6050078/1‑94 (MQ=60)
                            GCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCG                                                                   <  SRR4302545.2084046/94‑1 (MQ=60)
                             CCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGC                                                                  <  SRR4302545.10472974/94‑1 (MQ=60)
                             CCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGC                                                                  <  SRR4302545.10472975/94‑1 (MQ=60)
                             CCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGC                                                                  >  SRR4302545.9024680/1‑94 (MQ=60)
                             CCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGC                                                                  >  SRR4302545.3352618/1‑94 (MQ=60)
                              CCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCC                                                                 >  SRR4302545.9521001/1‑94 (MQ=60)
                              CCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCC                                                                 >  SRR4302545.9521002/1‑94 (MQ=60)
                               CTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTTGTGCGCCC                                                                <  SRR4302545.3357249/94‑1 (MQ=60)
                               CTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCC                                                                <  SRR4302545.1110036/94‑1 (MQ=60)
                               CTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCC                                                                >  SRR4302545.2738427/1‑94 (MQ=60)
                                 TTGCTATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGA                                                              <  SRR4302545.9832917/94‑1 (MQ=60)
                                  TGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGAT                                                             >  SRR4302545.20169/1‑94 (MQ=60)
                                   GCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATC                                                            >  SRR4302545.5986814/1‑94 (MQ=60)
                                   GCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATC                                                            >  SRR4302545.1120491/1‑94 (MQ=60)
                                   GCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATC                                                            <  SRR4302545.867106/94‑1 (MQ=60)
                                   GCCATCTTCTGTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATC                                                            <  SRR4302545.2575349/94‑1 (MQ=60)
                                     CATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGTGTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAC                                                          <  SRR4302545.1412925/94‑1 (MQ=60)
                                      ATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACC                                                         >  SRR4302545.8779288/1‑94 (MQ=60)
                                        CTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAT                                                       >  SRR4302545.85408/1‑94 (MQ=60)
                                         TTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATC                                                      <  SRR4302545.7808581/94‑1 (MQ=60)
                                          TCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCT                                                     >  SRR4302545.3592132/1‑94 (MQ=60)
                                          TCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCT                                                     <  SRR4302545.7183182/94‑1 (MQ=60)
                                          TCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCT                                                     >  SRR4302545.9758467/1‑94 (MQ=60)
                                            TTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCC                                                   <  SRR4302545.3550632/94‑1 (MQ=60)
                                            TTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCC                                                   <  SRR4302545.6944956/94‑1 (MQ=60)
                                            TTTCAAACCAACGCGGGTTAGGTGAGAGGTTTCCGGACGTTCGTCCCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCC                                                   <  SRR4302545.3550630/94‑1 (MQ=60)
                                             TTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCT                                                  >  SRR4302545.10567314/1‑94 (MQ=60)
                                             TTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCT                                                  >  SRR4302545.2215521/1‑94 (MQ=60)
                                              TCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTG                                                 <  SRR4302545.7139688/94‑1 (MQ=60)
                                              TCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTG                                                 >  SRR4302545.9102324/1‑94 (MQ=60)
                                              TCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTG                                                 <  SRR4302545.9984021/94‑1 (MQ=60)
                                              TCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTG                                                 <  SRR4302545.5329899/94‑1 (MQ=60)
                                              TCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTG                                                 <  SRR4302545.5329897/94‑1 (MQ=60)
                                               CAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGA                                                >  SRR4302545.3429903/1‑94 (MQ=60)
                                                AGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAT                                               <  SRR4302545.5395855/94‑1 (MQ=60)
                                                AGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAT                                               <  SRR4302545.10782508/94‑1 (MQ=60)
                                                AGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAT                                               >  SRR4302545.8472206/1‑94 (MQ=60)
                                                  ATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCG                                             <  SRR4302545.422975/94‑1 (MQ=60)
                                                  ATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCG                                             >  SRR4302545.6805859/1‑94 (MQ=60)
                                                  ATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCG                                             >  SRR4302545.9168231/1‑94 (MQ=60)
                                                    CAGCGCGGTTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTG                                           <  SRR4302545.9658295/94‑1 (MQ=60)
                                                     TACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGA                                          <  SRR4302545.2923047/94‑1 (MQ=60)
                                                      ACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAA                                         <  SRR4302545.10143149/94‑1 (MQ=60)
                                                      ACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAA                                         >  SRR4302545.9780043/1‑94 (MQ=60)
                                                      ACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAA                                         >  SRR4302545.9780296/1‑94 (MQ=60)
                                                       CGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAC                                        <  SRR4302545.9410110/94‑1 (MQ=60)
                                                       CGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAC                                        <  SRR4302545.10156963/94‑1 (MQ=60)
                                                          GGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCT                                     <  SRR4302545.6549923/94‑1 (MQ=60)
                                                          GGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCT                                     >  SRR4302545.9147586/1‑94 (MQ=60)
                                                          GGGTAAGGTGAGAAGTTTCCGGACGTACGTCGCCGCCGATCTCTACGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCT                                     <  SRR4302545.8292528/94‑1 (MQ=60)
                                                           GGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTC                                    <  SRR4302545.10123685/94‑1 (MQ=60)
                                                            GTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCA                                   <  SRR4302545.7701321/94‑1 (MQ=60)
                                                            GTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCA                                   <  SRR4302545.7564047/94‑1 (MQ=60)
                                                             TGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCAT                                  >  SRR4302545.7743709/1‑94 (MQ=60)
                                                             TGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCAT                                  <  SRR4302545.9016559/94‑1 (MQ=60)
                                                                GGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCG                               >  SRR4302545.5623334/1‑94 (MQ=60)
                                                                 GTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGG                              <  SRR4302545.3281638/94‑1 (MQ=60)
                                                                  TGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGT                             >  SRR4302545.5470149/1‑94 (MQ=60)
                                                                   GAGAGTTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTT                            <  SRR4302545.2038230/94‑1 (MQ=60)
                                                                   GAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTT                            >  SRR4302545.3947834/1‑94 (MQ=60)
                                                                   GAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTT                            <  SRR4302545.4002678/94‑1 (MQ=60)
                                                                   GAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTT                            <  SRR4302545.6346084/94‑1 (MQ=60)
                                                                    AGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTG                           <  SRR4302545.4387081/94‑1 (MQ=60)
                                                                      GGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAG                         <  SRR4302545.10132920/94‑1 (MQ=60)
                                                                      AGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAG                         >  SRR4302545.7397276/1‑94 (MQ=60)
                                                                      AGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAG                         <  SRR4302545.7429541/94‑1 (MQ=60)
                                                                        GTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCT                       >  SRR4302545.5903/1‑94 (MQ=60)
                                                                        GTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCT                       <  SRR4302545.9732890/94‑1 (MQ=60)
                                                                        GTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCT                       <  SRR4302545.4283123/94‑1 (MQ=60)
                                                                         TTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTG                      <  SRR4302545.2421315/94‑1 (MQ=60)
                                                                          TTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGC                     >  SRR4302545.2762928/1‑94 (MQ=60)
                                                                            CCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTT                   >  SRR4302545.5082899/1‑94 (MQ=60)
                                                                             CGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTC                  <  SRR4302545.2640209/94‑1 (MQ=60)
                                                                               gcgGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAG                <  SRR4302545.6048820/91‑1 (MQ=60)
                                                                               GACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAG                >  SRR4302545.10483385/1‑94 (MQ=60)
                                                                                  GTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTT             >  SRR4302545.7376622/1‑94 (MQ=60)
                                                                                   TTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTG            <  SRR4302545.10382738/94‑1 (MQ=60)
                                                                                   TGCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTG            <  SRR4302545.8580087/94‑1 (MQ=60)
                                                                                    TCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGT           <  SRR4302545.10549331/94‑1 (MQ=60)
                                                                                     CGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTG          >  SRR4302545.3743470/1‑94 (MQ=60)
                                                                                     CGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGGGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGGTGTG          >  SRR4302545.4498920/1‑94 (MQ=60)
                                                                                      GTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGT         >  SRR4302545.1207511/1‑94 (MQ=60)
                                                                                       TCGCCGCTGATCTATTCGCTAGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATTCGCTTGATATGCTTCAGGATGTGTT        >  SRR4302545.5967880/1‑94 (MQ=50)
                                                                                       TCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTT        <  SRR4302545.9160642/94‑1 (MQ=60)
                                                                                        CGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTC       <  SRR4302545.9433243/94‑1 (MQ=60)
                                                                                         GCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCC      <  SRR4302545.1901828/94‑1 (MQ=60)
                                                                                          CCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTAGAGCTGCTTCAGGTTGTGTTCCC     >  SRR4302545.7766001/1‑94 (MQ=60)
                                                                                          CCGCCGATCTCTTCGTTGGCCTCTGTGGTGCGCCTGATCTCCATCTCCTGATCGTGACCGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCC     <  SRR4302545.8500319/94‑1 (MQ=60)
                                                                                           CGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCA    >  SRR4302545.6178955/1‑94 (MQ=60)
                                                                                            GCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAA   >  SRR4302545.8587619/1‑94 (MQ=60)
                                                                                            GCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAA   >  SRR4302545.4563402/1‑94 (MQ=60)
                                                                                             CCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  >  SRR4302545.2054272/1‑94 (MQ=60)
                                                                                             CCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  <  SRR4302545.7039357/94‑1 (MQ=60)
                                                                                             CCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  >  SRR4302545.7000448/1‑94 (MQ=60)
                                                                                             |                                                                                             
TACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  >  NC_000913/2547856‑2548042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑