Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,547,949 T→C D206G (GAC→GGC)  b2431 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,547,9490TC100.0% 642.7 / NA 188D206G (GAC→GGC) b2431hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (91/97);  total (91/97)

Only 100 of 199 total aligned reads displayed.

CAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCC  >  NC_000913/2547861‑2548038
                                                                                        |                                                                                         
cAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAt                                                                                       <  1:1084607/93‑1 (MQ=255)
 aGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtc                                                                                      >  2:930391/1‑93 (MQ=255)
 aGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtc                                                                                      <  2:4005690/93‑1 (MQ=255)
  ggCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtct                                                                                     <  2:4984348/93‑1 (MQ=255)
  ggCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtct                                                                                     >  2:5313883/1‑93 (MQ=255)
   gCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAtctc                                                                                    >  2:3694582/1‑93 (MQ=255)
    ctctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATctct                                                                                   <  1:6264674/93‑1 (MQ=255)
      ctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGCTCTCTTc                                                                                 >  1:6180612/1‑93 (MQ=255)
      ctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTc                                                                                 <  2:4622517/93‑1 (MQ=255)
      ctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTc                                                                                 <  1:4699387/93‑1 (MQ=255)
       tGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCg                                                                                >  1:6012944/1‑93 (MQ=255)
        ggCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGt                                                                               <  1:5317010/93‑1 (MQ=255)
        ggCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGt                                                                               >  2:4156935/1‑93 (MQ=255)
        ggCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGt                                                                               <  2:6074130/93‑1 (MQ=255)
         gCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGtt                                                                              <  1:1019843/93‑1 (MQ=255)
           gAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCGACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTgg                                                                            <  2:2349671/93‑1 (MQ=255)
           gAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTgg                                                                            <  1:560288/93‑1 (MQ=255)
           gAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTgg                                                                            >  1:3035194/1‑93 (MQ=255)
              cAATCTTCAGCCCTTTGCCATCTTCTTCCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCct                                                                         <  2:5304523/93‑1 (MQ=255)
               aaTCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCctc                                                                        >  2:1686438/1‑93 (MQ=255)
               aaTCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCctc                                                                        >  2:2689434/1‑93 (MQ=255)
                gtCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTAGTCGCCGTCGAGCTCTTCGTTGGCctct                                                                       >  2:2378940/2‑93 (MQ=255)
                aTCTTCAGCCCTTTGCCATCTTCTTTCAGCTCAACGCGGGTGAGGCGAGAGGCTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTAGCctct                                                                       >  1:3241068/1‑93 (MQ=255)
                 tCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCtt                                                                      <  2:6011300/93‑1 (MQ=255)
                   ttCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTg                                                                    >  2:1825597/1‑93 (MQ=255)
                   ttCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTg                                                                    <  1:856547/93‑1 (MQ=255)
                    tCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTgg                                                                   >  2:6259337/1‑93 (MQ=255)
                    tCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTgg                                                                   <  2:4836559/93‑1 (MQ=255)
                       gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTgc                                                                >  2:493651/1‑93 (MQ=255)
                       gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTgc                                                                <  2:306410/93‑1 (MQ=255)
                       gCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTgc                                                                <  1:3800616/93‑1 (MQ=255)
                         ccTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTgcgc                                                              <  2:195655/93‑1 (MQ=255)
                          cTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGcc                                                             <  2:244219/93‑1 (MQ=255)
                           tttGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGccc                                                            >  1:2162323/1‑93 (MQ=255)
                            ttGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCg                                                           <  1:1498549/93‑1 (MQ=255)
                             tGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGa                                                          <  1:5242359/93‑1 (MQ=255)
                             tGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGa                                                          <  1:1966989/93‑1 (MQ=255)
                              gCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTAGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGAt                                                         >  2:4022908/1‑93 (MQ=255)
                               ccATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATc                                                        <  1:3918660/93‑1 (MQ=255)
                               ccATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATc                                                        >  1:5766327/1‑93 (MQ=255)
                                cATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa                                                       <  1:1178177/93‑1 (MQ=255)
                                cATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa                                                       <  1:5416232/93‑1 (MQ=255)
                                cATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCa                                                       <  1:3096447/93‑1 (MQ=255)
                                  tcttctTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAcc                                                     >  2:3817194/1‑93 (MQ=255)
                                   cttcttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCa                                                    >  1:2872367/1‑93 (MQ=255)
                                         tCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCt                                              >  1:3078458/1‑93 (MQ=255)
                                         tCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCt                                              <  1:2902941/93‑1 (MQ=255)
                                         tCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCt                                              >  1:4058775/1‑93 (MQ=255)
                                         tCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCt                                              >  1:6044697/1‑93 (MQ=255)
                                           aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGa                                            <  2:3435886/93‑1 (MQ=255)
                                           aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGa                                            <  1:3322515/93‑1 (MQ=255)
                                           aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGGTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGa                                            >  1:3784488/1‑93 (MQ=255)
                                            gATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCGGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAt                                           >  1:4552588/1‑93 (MQ=255)
                                            gATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAt                                           <  1:3488824/93‑1 (MQ=255)
                                            gATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAt                                           >  2:2189909/1‑93 (MQ=255)
                                              tCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCg                                         >  1:4519512/1‑93 (MQ=255)
                                                 aCGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGa                                      >  2:3801982/1‑93 (MQ=255)
                                                 aCGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGa                                      >  1:670324/1‑93 (MQ=255)
                                                 aCGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGa                                      <  2:4731186/93‑1 (MQ=255)
                                                  cgcgGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGaa                                     <  2:2669255/93‑1 (MQ=255)
                                                  cgcgGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGaa                                     <  1:2813411/93‑1 (MQ=255)
                                                   gcgGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAc                                    <  2:686727/93‑1 (MQ=255)
                                                      ggtgaggtgaGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCt                                 <  1:967214/93‑1 (MQ=255)
                                                      ggtgaggtgaGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCt                                 <  1:1635507/93‑1 (MQ=255)
                                                         gaggtgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCAt                              >  2:853475/1‑93 (MQ=255)
                                                           ggtgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATcc                            <  1:3900079/93‑1 (MQ=255)
                                                             tgagAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCgg                          >  1:5845199/1‑93 (MQ=255)
                                                             ggagaGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCgg                          <  2:4531993/92‑1 (MQ=255)
                                                               ggagGTTTCCGCACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGtt                        <  2:2410836/92‑1 (MQ=255)
                                                               agagGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGtt                        <  2:552921/93‑1 (MQ=255)
                                                               agagGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGtt                        >  1:3543340/1‑93 (MQ=255)
                                                                gagGTTTCGGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTg                       <  1:130232/93‑1 (MQ=255)
                                                                 agGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGa                      <  2:4315811/93‑1 (MQ=255)
                                                                 agGGTTCCGGACGTTCGTCGCCGCCGGTCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGCACGCTCATCCGGCTGa                      >  2:1230535/1‑93 (MQ=255)
                                                                  ggTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                     >  2:5615980/1‑93 (MQ=255)
                                                                  ggTTTCCGGACGTTCGTCGCCGCCAATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                     >  2:107261/1‑93 (MQ=255)
                                                                  gctttCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                     <  1:5052173/91‑1 (MQ=255)
                                                                   gTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgc                    >  1:6425280/1‑93 (MQ=255)
                                                                   gTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgc                    >  2:2755882/1‑93 (MQ=255)
                                                                   gTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgc                    <  2:324031/93‑1 (MQ=255)
                                                                    tttCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                   <  2:132219/93‑1 (MQ=255)
                                                                    tttCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                   >  1:4598195/1‑93 (MQ=255)
                                                                    tttCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                   <  1:3827730/93‑1 (MQ=255)
                                                                     ttCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgctg                  <  1:3992299/93‑1 (MQ=255)
                                                                      tCCGGACGTTCGTCGCCGCCGATCTCTTCGTCGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgctgc                 <  2:5387385/93‑1 (MQ=255)
                                                                        cGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCtt               <  1:1706493/93‑1 (MQ=255)
                                                                        cGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCtt               >  2:5131748/1‑93 (MQ=255)
                                                                        cGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGATCATCCGGTTGAGCTGCtt               >  2:2811748/1‑93 (MQ=255)
                                                                         caaCGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTc              <  2:4219366/91‑1 (MQ=255)
                                                                          gACGTCCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCa             <  2:5452502/93‑1 (MQ=255)
                                                                           gcGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAg            <  1:3296416/92‑1 (MQ=255)
                                                                           aCGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAg            <  1:5976982/93‑1 (MQ=255)
                                                                             gTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGt          >  1:4808412/1‑93 (MQ=255)
                                                                             gTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGt          <  1:2433059/93‑1 (MQ=255)
                                                                             gTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAAGCTCATCCGGTTGAGCTGCTTCAGGt          >  2:2981177/1‑93 (MQ=255)
                                                                              ttCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGtt         <  1:1461397/93‑1 (MQ=255)
                                                                              ttCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGtt         >  1:2354150/1‑93 (MQ=255)
                                                                                 gtcgCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTtgtg      >  2:5697014/1‑93 (MQ=255)
                                                                                 gtcgCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTtgtg      <  2:1508725/93‑1 (MQ=255)
                                                                                    gccgccGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTc   <  1:5104113/93‑1 (MQ=255)
                                                                                        |                                                                                         
CAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCC  >  NC_000913/2547861‑2548038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  >  NC_000913/2547860‑2548042
                                                                                         |                                                                                             
GCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGAT                                                                                           >  SRR4302537.3061849/1‑94 (MQ=60)
 CAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATC                                                                                          <  SRR4302537.1092090/94‑1 (MQ=60)
 CAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATC                                                                                          >  SRR4302537.1267725/1‑94 (MQ=60)
   GGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTC                                                                                        <  SRR4302537.3282053/94‑1 (MQ=60)
   GGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTC                                                                                        <  SRR4302537.6115338/94‑1 (MQ=60)
     CTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTT                                                                                      >  SRR4302537.2707368/1‑94 (MQ=60)
     CTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTT                                                                                      <  SRR4302537.6307676/94‑1 (MQ=60)
      TCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGCTCTCTTC                                                                                     >  SRR4302537.6223185/1‑94 (MQ=60)
       CTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCG                                                                                    >  SRR4302537.6054256/1‑94 (MQ=60)
       CTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCG                                                                                    <  SRR4302537.4731898/94‑1 (MQ=60)
         GGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTT                                                                                  <  SRR4302537.5353516/94‑1 (MQ=60)
          GCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTG                                                                                 <  SRR4302537.355039/94‑1 (MQ=60)
          GCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTG                                                                                 <  SRR4302537.1026873/94‑1 (MQ=60)
           CGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGG                                                                                >  SRR4302537.4151427/1‑94 (MQ=60)
           CGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGG                                                                                >  SRR4302537.3056352/1‑94 (MQ=60)
           CGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGC                                                                                >  SRR4302537.934049/1‑94 (MQ=60)
           CAAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACTTTCGTCGCCGCCGATCTCTTCGTTGG                                                                                <  SRR4302537.3503609/94‑1 (MQ=60)
            GAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGC                                                                               >  SRR4302537.4054139/1‑94 (MQ=60)
            GAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGC                                                                               <  SRR4302537.564147/94‑1 (MQ=60)
             AACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCC                                                                              >  SRR4302537.4710899/1‑94 (MQ=60)
               CAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTC                                                                            >  SRR4302537.5408223/1‑94 (MQ=60)
                AATCTTCAGCCCTTTGCCATCTTCTTTCAGCTCAACGCGGGTGAGGCGAGAGGCTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTAGCCTCT                                                                           >  SRR4302537.3263911/1‑94 (MQ=60)
                AATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCT                                                                           >  SRR4302537.393384/1‑94 (MQ=60)
                    TTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGG                                                                       <  SRR4302537.862427/94‑1 (MQ=60)
                    TTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGG                                                                       <  SRR4302537.5255226/94‑1 (MQ=60)
                       AGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGC                                                                    <  SRR4302537.3430908/94‑1 (MQ=60)
                       AGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGC                                                                    <  SRR4302537.6074744/94‑1 (MQ=60)
                        GCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCG                                                                   <  SRR4302537.3827281/94‑1 (MQ=60)
                        GCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCG                                                                   >  SRR4302537.2305361/1‑94 (MQ=60)
                          CCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCC                                                                 >  SRR4302537.3910096/1‑94 (MQ=60)
                           CTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCG                                                                >  SRR4302537.6422936/1‑94 (MQ=60)
                           CTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCC                                                                >  SRR4302537.3827971/1‑94 (MQ=60)
                           CTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCC                                                                >  SRR4302537.2177561/1‑94 (MQ=60)
                             TTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGA                                                              <  SRR4302537.1509008/94‑1 (MQ=60)
                              TGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGAT                                                             <  SRR4302537.5278437/94‑1 (MQ=60)
                              TGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGAT                                                             <  SRR4302537.1980667/94‑1 (MQ=60)
                               GCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATC                                                            >  SRR4302537.5805824/1‑94 (MQ=60)
                               GCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATC                                                            >  SRR4302537.1668975/1‑94 (MQ=60)
                                CCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCA                                                           <  SRR4302537.3946051/94‑1 (MQ=60)
                                 CATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAC                                                          <  SRR4302537.1186369/94‑1 (MQ=60)
                                 CATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAC                                                          <  SRR4302537.3118057/94‑1 (MQ=60)
                                 CATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAC                                                          <  SRR4302537.5453159/94‑1 (MQ=60)
                                   TCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCA                                                        >  SRR4302537.2892525/1‑94 (MQ=60)
                                     TTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATC                                                      <  SRR4302537.1158922/94‑1 (MQ=60)
                                        TTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCC                                                   >  SRR4302537.143115/1‑94 (MQ=60)
                                         TTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCT                                                  >  SRR4302537.4087141/1‑94 (MQ=60)
                                         TTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCT                                                  >  SRR4302537.3099901/1‑94 (MQ=60)
                                         TTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCT                                                  >  SRR4302537.6086212/1‑94 (MQ=60)
                                          TCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTG                                                 <  SRR4302537.2923336/94‑1 (MQ=60)
                                           CAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGGTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGA                                                >  SRR4302537.3811058/1‑94 (MQ=60)
                                            AGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCGGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAT                                               >  SRR4302537.4584092/1‑94 (MQ=60)
                                            AGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAT                                               <  SRR4302537.3346019/94‑1 (MQ=60)
                                            AGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGAT                                               >  SRR4302537.851171/1‑94 (MQ=60)
                                             GATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATC                                              <  SRR4302537.3513501/94‑1 (MQ=60)
                                             GATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATC                                              >  SRR4302537.3047496/1‑94 (MQ=60)
                                              ATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCT                                             >  SRR4302537.1873441/1‑94 (MQ=60)
                                              ATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCG                                             >  SRR4302537.4550771/1‑94 (MQ=60)
                                                 AACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGA                                          >  SRR4302537.3224966/1‑94 (MQ=60)
                                                 AACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGA                                          >  SRR4302537.2857036/1‑94 (MQ=60)
                                                 AACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGA                                          >  SRR4302537.674906/1‑94 (MQ=60)
                                                   CGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAC                                        <  SRR4302537.993010/94‑1 (MQ=60)
                                                   CGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAC                                        >  SRR4302537.2435940/1‑94 (MQ=60)
                                                   CGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAAC                                        <  SRR4302537.2833083/94‑1 (MQ=60)
                                                    GCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACG                                       <  SRR4302537.4004468/94‑1 (MQ=60)
                                                    GCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACG                                       <  SRR4302537.2161375/94‑1 (MQ=60)
                                                       GGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTC                                    <  SRR4302537.973882/94‑1 (MQ=60)
                                                       GGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTC                                    >  SRR4302537.1479767/1‑94 (MQ=60)
                                                       GGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTC                                    <  SRR4302537.1646961/94‑1 (MQ=60)
                                                          GAGGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATC                                 >  SRR4302537.2899949/1‑94 (MQ=60)
                                                            GGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCG                               <  SRR4302537.5962412/94‑1 (MQ=60)
                                                            GGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCG                               <  SRR4302537.3927345/94‑1 (MQ=60)
                                                            GGTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCG                               <  SRR4302537.4770937/94‑1 (MQ=60)
                                                             GTGAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGG                              >  SRR4302537.5885280/1‑94 (MQ=60)
                                                               GAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTT                            >  SRR4302537.3103505/1‑94 (MQ=60)
                                                               GAGAGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTT                            >  SRR4302537.3568371/1‑94 (MQ=60)
                                                                 GAGGTTTCGGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGA                          <  SRR4302537.131155/94‑1 (MQ=60)
                                                                  AGGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAG                         <  SRR4302537.6052547/94‑1 (MQ=60)
                                                                   GGTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGC                        >  SRR4302537.6468941/1‑94 (MQ=60)
                                                                   GCTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGC                        <  SRR4302537.5086957/94‑1 (MQ=60)
                                                                    GTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCT                       <  SRR4302537.5504198/94‑1 (MQ=60)
                                                                    GTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCT                       >  SRR4302537.6275012/1‑94 (MQ=60)
                                                                    GTTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCT                       >  SRR4302537.4630017/1‑94 (MQ=60)
                                                                     TTTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTG                      <  SRR4302537.3854568/94‑1 (MQ=60)
                                                                      TTCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGC                     <  SRR4302537.4020250/94‑1 (MQ=60)
                                                                       TCCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCT                    <  SRR4302537.3651690/94‑1 (MQ=60)
                                                                        CCGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTT                   >  SRR4302537.3822243/1‑94 (MQ=60)
                                                                         CGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTC                  <  SRR4302537.1718421/94‑1 (MQ=60)
                                                                         CGGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTC                  >  SRR4302537.5336475/1‑94 (MQ=60)
                                                                          GGACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCA                 >  SRR4302537.4613701/1‑94 (MQ=60)
                                                                            TCGTTCGTCGCCCCCGATCTCTTCGTTTGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGG               <  SRR4302537.5668032/94‑1 (MQ=60)
                                                                            GCGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGG               <  SRR4302537.3319717/94‑1 (MQ=60)
                                                                            ACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGG               <  SRR4302537.6018013/94‑1 (MQ=60)
                                                                            ACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGG               <  SRR4302537.3328755/94‑1 (MQ=60)
                                                                            ACGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGG               <  SRR4302537.6520889/94‑1 (MQ=60)
                                                                             CGTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGT              >  SRR4302537.4841600/1‑94 (MQ=60)
                                                                              GTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTT             <  SRR4302537.2450200/94‑1 (MQ=60)
                                                                              GTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTT             >  SRR4302537.2370750/1‑94 (MQ=60)
                                                                              GTTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTT             <  SRR4302537.1569230/94‑1 (MQ=60)
                                                                               TTCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTG            <  SRR4302537.1471565/94‑1 (MQ=60)
                                                                                TCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGT           >  SRR4302537.5117455/1‑94 (MQ=60)
                                                                                TCGTCGCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGT           <  SRR4302537.457333/94‑1 (MQ=60)
                                                                                   TCCCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTT        <  SRR4302537.5073674/94‑1 (MQ=60)
                                                                                     GCCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCC      <  SRR4302537.5139287/94‑1 (MQ=60)
                                                                                      CCGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCC     <  SRR4302537.58138/94‑1 (MQ=60)
                                                                                       CGCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCA    >  SRR4302537.5394288/1‑94 (MQ=60)
                                                                                        GCCGATCTCTTTGTTGGCCTCTTTGGTGCGCACGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGGTGCTTCAGGTTGTGTTCCCAA   <  SRR4302537.6165358/94‑1 (MQ=60)
                                                                                        GCCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAA   <  SRR4302537.1942249/94‑1 (MQ=60)
                                                                                         CCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  >  SRR4302537.1730126/1‑94 (MQ=60)
                                                                                         CCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  >  SRR4302537.408683/1‑94 (MQ=60)
                                                                                         |                                                                                             
GCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAAC  >  NC_000913/2547860‑2548042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑