Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,551,423 T→C V60A (GTC→GCC)  hemF → coproporphyrinogen III oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,551,4230TC98.0% 845.4 / 1.8 254V60A (GTC→GCC) hemFcoproporphyrinogen III oxidase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (110/139);  total (112/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 267 total aligned reads displayed.

GAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGG  >  NC_000913/2551332‑2551513
                                                                                           |                                                                                          
gAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCa                                                                                          <  1:1334450/94‑1 (MQ=255)
gAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCa                                                                                          <  1:9232132/94‑1 (MQ=255)
 aaTTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCaa                                                                                         <  2:3143183/94‑1 (MQ=255)
 aaTTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGGAATGGTGGTGTTTTCGAACAAGCAGGCGCCaa                                                                                         >  1:3960212/1‑94 (MQ=255)
     tGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGGGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACttt                                                                                     >  2:4696064/1‑94 (MQ=255)
      gTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACtttt                                                                                    >  1:5099993/1‑94 (MQ=255)
       tCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTc                                                                                   <  1:3744694/94‑1 (MQ=255)
       tCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTc                                                                                   <  1:4617188/94‑1 (MQ=255)
        cGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCCACTTTTCg                                                                                  >  1:2440395/1‑94 (MQ=255)
           aGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCAt                                                                               >  2:4454986/1‑94 (MQ=255)
           aGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGGGTTTTCGAACAGGCAGGCGCCAACTTTTCGCAt                                                                               >  2:2809556/1‑94 (MQ=255)
             aTAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGt                                                                             <  1:10274561/94‑1 (MQ=255)
             aTAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGt                                                                             <  2:4232904/94‑1 (MQ=255)
               aGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTca                                                                           <  1:1834615/94‑2 (MQ=255)
                gTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCa                                                                          <  2:5750492/94‑1 (MQ=255)
                  tGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACg                                                                        <  2:1238815/94‑1 (MQ=255)
                   ggCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACgg                                                                       <  2:663127/94‑1 (MQ=255)
                   ggCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACgg                                                                       <  2:9315681/94‑1 (MQ=255)
                   ggCAGCGCGAAGCTGGCGGCGGCGGGCGGAGTCGGGTGTTGCGGAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACgg                                                                       >  1:9446449/1‑94 (MQ=255)
                    gCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGt                                                                      <  1:10034023/94‑1 (MQ=255)
                    gCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGt                                                                      <  1:8911982/94‑1 (MQ=255)
                    gCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGt                                                                      >  2:3000359/1‑94 (MQ=255)
                     taGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTg                                                                     >  1:3087959/2‑94 (MQ=255)
                     cAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTg                                                                     <  2:5831516/94‑1 (MQ=255)
                      aGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGa                                                                    >  2:1703450/1‑94 (MQ=255)
                        cgcgAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCCTGTCCACGGTGAgg                                                                  >  1:3114960/1‑94 (MQ=255)
                        cgcgAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAgg                                                                  >  2:3463189/1‑94 (MQ=255)
                        cgcgAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAgg                                                                  <  2:623715/94‑1 (MQ=255)
                          cgAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCg                                                                <  1:501440/94‑1 (MQ=255)
                          cgAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCg                                                                <  2:8493367/94‑1 (MQ=255)
                           gAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGa                                                               >  1:10157091/1‑94 (MQ=255)
                             aGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATg                                                             <  1:986384/94‑1 (MQ=255)
                             aGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATg                                                             <  1:2343883/94‑1 (MQ=255)
                             aGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATg                                                             <  1:6598812/94‑1 (MQ=255)
                             aGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATg                                                             <  1:6894123/94‑1 (MQ=255)
                                tGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCt                                                          <  1:8856131/94‑1 (MQ=255)
                                tGGCGGCGGCGGGCATAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCt                                                          >  2:4017629/1‑94 (MQ=255)
                                cggcggcggcGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCt                                                          <  2:6969440/93‑1 (MQ=255)
                                 ggcggcggcGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTg                                                         <  2:3725920/94‑1 (MQ=255)
                                  gcggcggcgGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGc                                                        <  1:7171171/94‑1 (MQ=255)
                                   cggcggcggGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCt                                                       <  1:580195/94‑1 (MQ=255)
                                   cggcggcggGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCt                                                       >  2:2248159/1‑94 (MQ=255)
                                    ggcggcggGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGGGAGGCGATGCCTGCtt                                                      >  1:4732088/1‑94 (MQ=255)
                                     gcggcggGCGTAGTCGGGGGTTGCGTAATGGGGGGGGTTTCGAACAGGCAGGCGCCCACTTTTCGCATGTCCACGGGGAGGGGATGCCTGCTTc                                                     >  2:7961061/1‑94 (MQ=255)
                                      cggcggGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTcc                                                    <  1:5972691/94‑1 (MQ=255)
                                        gcgggcgTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGc                                                  <  2:1564685/94‑1 (MQ=255)
                                        gcgggcgTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGc                                                  >  2:520255/1‑94 (MQ=255)
                                         cgggcgTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGcc                                                 >  2:2216160/1‑94 (MQ=255)
                                         cgggcgTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGcc                                                 >  2:2979259/1‑94 (MQ=255)
                                             ggTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCg                                             <  1:8113498/93‑1 (MQ=255)
                                              gTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGc                                            >  1:515617/1‑94 (MQ=255)
                                               tAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCt                                           <  2:10433916/94‑1 (MQ=255)
                                                 gTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCa                                         <  1:2196734/94‑1 (MQ=255)
                                                  tCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCAt                                        <  2:6277832/94‑1 (MQ=255)
                                                   cGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATc                                       >  2:1380381/1‑94 (MQ=255)
                                                   cGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATc                                       <  2:6865114/94‑1 (MQ=255)
                                                    gggTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCg                                      >  1:8386964/1‑94 (MQ=255)
                                                    gggTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCg                                      >  2:2702886/1‑94 (MQ=255)
                                                     ggTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGc                                     >  1:9698201/1‑94 (MQ=255)
                                                     ggTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGc                                     <  1:7542855/94‑1 (MQ=255)
                                                      gtgtTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGcc                                    >  1:2612697/1‑94 (MQ=255)
                                                      gtgtTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGcc                                    >  1:3834578/1‑94 (MQ=255)
                                                       tgtTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGccc                                   >  2:9826202/1‑94 (MQ=255)
                                                       tgtTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGccc                                   <  2:7476244/94‑1 (MQ=255)
                                                          tGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGa                                <  1:8258925/94‑1 (MQ=255)
                                                          tGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGa                                >  1:3098492/1‑94 (MQ=255)
                                                            ggTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAAc                              <  2:8994569/93‑1 (MQ=255)
                                                            cGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTACGCCACCGCTCATCGCCCGGAAc                              >  2:9168889/1‑94 (MQ=255)
                                                             gTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACt                             >  1:1369401/1‑94 (MQ=255)
                                                               actggtggTGTTTTCGAACAGGCAGGCGCCAACTTTTTGCATGTGCACGGTGACGCGGTGGGTGCGTCCGCCGCCCCTCATCGCCCGGAACttt                           >  2:7094464/3‑93 (MQ=255)
                                                                  ggtggtGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCg                        <  1:7671160/94‑1 (MQ=255)
                                                                   gtggtgTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCgg                       >  1:3711876/1‑94 (MQ=255)
                                                                   gtggtgTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCgg                       <  1:9332949/94‑1 (MQ=255)
                                                                    tggtgTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCggg                      >  1:2887212/1‑94 (MQ=255)
                                                                     ggtgTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGCACTTGCCGGgc                     <  1:6864105/94‑1 (MQ=255)
                                                                     ggtgTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGgc                     >  2:4094561/1‑94 (MQ=255)
                                                                       tgtTTTCGAACAGGCAGGCGTCAACTTTTCGCACGTCAAAGGCGAGGCGATGCCTGCTTCCGCAACCACTCATCGCCCGGCACTGGCCgcgcgc                   >  2:9456856/1‑94 (MQ=255)
                                                                       tgtTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGgcgc                   >  1:3016143/1‑94 (MQ=255)
                                                                       tgtTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGgcgc                   >  2:10184723/1‑94 (MQ=255)
                                                                        gtTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCa                  <  1:1758955/94‑1 (MQ=255)
                                                                         ttttCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAg                 <  2:7366584/94‑1 (MQ=255)
                                                                          tttCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGt                >  1:151831/1‑94 (MQ=255)
                                                                           ttCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGtt               <  1:9093771/94‑1 (MQ=255)
                                                                            tCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGttt              >  2:827289/1‑94 (MQ=255)
                                                                            tCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGttt              <  1:4199649/94‑1 (MQ=255)
                                                                                 caggcaggCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAgg         >  2:2344971/1‑94 (MQ=255)
                                                                                  aggcaggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGc        >  1:6127904/1‑94 (MQ=255)
                                                                                  aggcaggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGc        <  2:7849341/94‑1 (MQ=255)
                                                                                  aggcaggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGc        >  1:445086/1‑94 (MQ=255)
                                                                                  aggcaggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGc        >  1:7907175/1‑94 (MQ=255)
                                                                                     caggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGAt     >  2:8610211/1‑94 (MQ=255)
                                                                                     caggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGAt     <  2:5577162/94‑1 (MQ=255)
                                                                                     caggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCCGAACTTGCCGGGCGCGGTTTCGAGGCGag     >  1:2011235/1‑93 (MQ=255)
                                                                                      aggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATg    <  1:4882894/94‑1 (MQ=255)
                                                                                      aggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATg    <  2:1118214/94‑1 (MQ=255)
                                                                                      aggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATg    >  2:2509237/1‑94 (MQ=255)
                                                                                       ggcGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATgg   <  2:7299104/94‑1 (MQ=255)
                                                                                        gcgcCACCTGTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATggg  <  1:5355587/94‑1 (MQ=255)
                                                                                        gcgcCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATggg  <  2:5914871/94‑1 (MQ=255)
                                                                                        gcgcCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCACGTAACTTGCCGGGCGCCGTTTCGGGGCGGTggg  >  1:4011530/1‑94 (MQ=255)
                                                                                           |                                                                                          
GAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGG  >  NC_000913/2551332‑2551513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGGCGTT  >  NC_000913/2551330‑2551517
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CAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTGATGGTGGTGTTTTCGAACAGGGAGGCGC                                                                                                >  SRR4302545.10283499/1‑94 (MQ=60)
 AGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCC                                                                                               >  SRR4302545.6646604/1‑94 (MQ=60)
 AGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCC                                                                                               >  SRR4302545.5509734/1‑94 (MQ=60)
 AGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGCGTTTTCGAACAGGAAGGCGCC                                                                                               >  SRR4302545.563770/1‑94 (MQ=60)
  GAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCA                                                                                              <  SRR4302545.2458232/94‑1 (MQ=60)
  GAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCA                                                                                              <  SRR4302545.1364170/94‑1 (MQ=60)
  GAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCA                                                                                              <  SRR4302545.9502808/94‑1 (MQ=60)
   AATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGGAATGGTGGTGTTTTCGAACAAGCAGGCGCCAA                                                                                             >  SRR4302545.4059196/1‑94 (MQ=60)
    ATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAAC                                                                                            >  SRR4302545.92203/1‑94 (MQ=60)
     TTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACT                                                                                           <  SRR4302545.7039031/94‑1 (MQ=60)
     TTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACT                                                                                           <  SRR4302545.7671492/94‑1 (MQ=60)
       TGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTT                                                                                         >  SRR4302545.9614652/1‑94 (MQ=60)
        GTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTT                                                                                        >  SRR4302545.5230229/1‑94 (MQ=60)
        GTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTT                                                                                        <  SRR4302545.8314977/94‑1 (MQ=60)
        GTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGGGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTT                                                                                        >  SRR4302545.2489393/1‑94 (MQ=60)
         TCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTC                                                                                       <  SRR4302545.4735292/94‑1 (MQ=60)
         TCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTC                                                                                       <  SRR4302545.3836878/94‑1 (MQ=60)
         TCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTC                                                                                       <  SRR4302545.3836876/94‑1 (MQ=60)
         TCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTC                                                                                       <  SRR4302545.73132/94‑1 (MQ=60)
          CGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCCACTTTTCG                                                                                      >  SRR4302545.2499514/1‑94 (MQ=60)
            AAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTTGCA                                                                                    >  SRR4302545.5230321/1‑94 (MQ=60)
             AGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCAT                                                                                   <  SRR4302545.920052/94‑1 (MQ=60)
              GATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATG                                                                                  <  SRR4302545.5940512/94‑1 (MQ=60)
               ATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGT                                                                                 <  SRR4302545.10577640/94‑1 (MQ=60)
                TAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTC                                                                                <  SRR4302545.3716163/94‑1 (MQ=60)
                 AGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCA                                                                               <  SRR4302545.1879213/94‑1 (MQ=60)
                   TTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCAC                                                                             <  SRR4302545.8143974/94‑1 (MQ=60)
                     TGCAGCGCGAAGCTGGCGGCGGCGGGTGTAGTCGGGTGTTGCGTAATGGTGGGGGTTTCGAACAGGCTGGCGCCAACTTTTCGCATGTCCACGG                                                                           >  SRR4302545.9658097/1‑94 (MQ=60)
                     GGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGG                                                                           >  SRR4302545.9515324/1‑94 (MQ=60)
                     GGCAGCGCGAAGCTGGCGGCGGCGGGCGGAGTCGGGTGTTGCGGAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGG                                                                           >  SRR4302545.9724114/1‑94 (MQ=60)
                      GCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGT                                                                          <  SRR4302545.9171067/94‑1 (MQ=60)
                      GCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGT                                                                          <  SRR4302545.9154167/94‑1 (MQ=60)
                      GCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGT                                                                          <  SRR4302545.10331273/94‑1 (MQ=60)
                       TAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTG                                                                         >  SRR4302545.3163952/1‑94 (MQ=60)
                       CAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTG                                                                         <  SRR4302545.4246662/94‑1 (MQ=60)
                        AGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGA                                                                        <  SRR4302545.8544815/94‑1 (MQ=60)
                        AGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGGGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGA                                                                        >  SRR4302545.2640183/1‑94 (MQ=60)
                         GCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAG                                                                       <  SRR4302545.462298/94‑1 (MQ=60)
                         GCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAG                                                                       <  SRR4302545.4768155/94‑1 (MQ=60)
                         GCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAG                                                                       <  SRR4302545.3099009/94‑1 (MQ=60)
                          CGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGACCAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGG                                                                      <  SRR4302545.1524417/94‑1 (MQ=60)
                          CGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCCTGTCCACGGTGAGG                                                                      >  SRR4302545.3191779/1‑94 (MQ=60)
                          CGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGG                                                                      >  SRR4302545.1373606/1‑94 (MQ=60)
                          CGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGG                                                                      >  SRR4302545.3191475/1‑94 (MQ=60)
                          CGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGG                                                                      <  SRR4302545.1524418/94‑1 (MQ=60)
                          CGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAAATTTTCGCATGTCCACGGTGAGG                                                                      >  SRR4302545.2944031/1‑94 (MQ=60)
                           GCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTAGAACAGGCAGGCGCCATCTTTTCGCATGTCCACGGTGAGGC                                                                     <  SRR4302545.3166980/94‑1 (MQ=60)
                            CGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCG                                                                    <  SRR4302545.512682/94‑1 (MQ=60)
                             GAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGA                                                                   >  SRR4302545.10457393/1‑94 (MQ=60)
                               AGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATG                                                                 <  SRR4302545.6779475/94‑1 (MQ=60)
                               AGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATG                                                                 <  SRR4302545.1008330/94‑1 (MQ=60)
                               AGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATG                                                                 <  SRR4302545.8583403/94‑1 (MQ=60)
                               AGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATG                                                                 <  SRR4302545.2400937/94‑1 (MQ=60)
                               AGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATG                                                                 <  SRR4302545.7083822/94‑1 (MQ=60)
                                  TGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCT                                                              <  SRR4302545.9113379/94‑1 (MQ=60)
                                   GGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTG                                                             >  SRR4302545.1018160/1‑94 (MQ=60)
                                    GCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGC                                                            <  SRR4302545.7370364/94‑1 (MQ=60)
                                     CGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCT                                                           <  SRR4302545.593299/94‑1 (MQ=60)
                                      GGCTGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTT                                                          <  SRR4302545.2500454/94‑1 (MQ=60)
                                      GGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTT                                                          <  SRR4302545.10006625/94‑1 (MQ=60)
                                      GGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTT                                                          <  SRR4302545.4354427/94‑1 (MQ=60)
                                      GGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTT                                                          <  SRR4302545.3859675/94‑1 (MQ=60)
                                      GGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGGGAGGCGATGCCTGCTT                                                          >  SRR4302545.4852789/1‑94 (MQ=60)
                                       GCGGCGGGCGTAGTCGGGGGTTGCGTAATGGTGGTGTTTTCGAACAGGGAGGGGCCCACTTTTCGCATGTCCACCGGGAGGCGGTGCCTGCTTC                                                         >  SRR4302545.4182486/1‑94 (MQ=60)
                                        CGGGGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCC                                                        <  SRR4302545.2039472/94‑1 (MQ=60)
                                        CGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCC                                                        <  SRR4302545.6133810/94‑1 (MQ=60)
                                           CGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCC                                                     >  SRR4302545.8087908/1‑94 (MQ=60)
                                           CGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCC                                                     >  SRR4302545.2702752/1‑94 (MQ=60)
                                            GGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCC                                                    >  SRR4302545.5070878/1‑94 (MQ=60)
                                            GGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCA                                                    <  SRR4302545.7079853/94‑1 (MQ=60)
                                             GGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGATTCCGCCAC                                                   >  SRR4302545.4247647/1‑94 (MQ=60)
                                              GCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACC                                                  >  SRR4302545.8483986/1‑94 (MQ=60)
                                               GGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCG                                                 <  SRR4302545.8347581/94‑1 (MQ=60)
                                               CGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCG                                                 >  SRR4302545.3991561/1‑94 (MQ=60)
                                               CGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCG                                                 >  SRR4302545.1306785/1‑94 (MQ=60)
                                                GTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGC                                                >  SRR4302545.191699/1‑94 (MQ=60)
                                                GTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGC                                                >  SRR4302545.527145/1‑94 (MQ=60)
                                                GTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGC                                                >  SRR4302545.6612738/1‑94 (MQ=60)
                                                 TAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCT                                               <  SRR4302545.1075737/94‑1 (MQ=60)
                                                  AGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTC                                              >  SRR4302545.4339251/1‑94 (MQ=60)
                                                  AGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTC                                              >  SRR4302545.3181094/1‑94 (MQ=60)
                                                   GTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCA                                             <  SRR4302545.2250413/94‑1 (MQ=60)
                                                   GTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCA                                             >  SRR4302545.3778044/1‑94 (MQ=60)
                                                    TCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCAT                                            <  SRR4302545.858001/94‑1 (MQ=60)
                                                     CGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATC                                           >  SRR4302545.4940082/1‑94 (MQ=60)
                                                     CGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATC                                           <  SRR4302545.5327661/94‑1 (MQ=60)
                                                      GGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCG                                          >  SRR4302545.8629757/1‑94 (MQ=60)
                                                       GGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGC                                         <  SRR4302545.7756149/94‑1 (MQ=60)
                                                       GGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGC                                         >  SRR4302545.6144268/1‑94 (MQ=60)
                                                       GGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGC                                         >  SRR4302545.9986508/1‑94 (MQ=60)
                                                        GTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCC                                        >  SRR4302545.2675584/1‑94 (MQ=60)
                                                        GTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCC                                        >  SRR4302545.3930180/1‑94 (MQ=60)
                                                         TGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCC                                       <  SRR4302545.4831509/94‑1 (MQ=60)
                                                         TGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCC                                       >  SRR4302545.2327895/1‑94 (MQ=60)
                                                         TGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCC                                       >  SRR4302545.4060687/1‑94 (MQ=60)
                                                           TTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGG                                     <  SRR4302545.7171314/94‑1 (MQ=60)
                                                            TGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGA                                    <  SRR4302545.8497966/94‑1 (MQ=60)
                                                            TGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGA                                    <  SRR4302545.5896234/94‑1 (MQ=60)
                                                            TGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGA                                    >  SRR4302545.3174805/1‑94 (MQ=60)
                                                             GCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAA                                   >  SRR4302545.5229131/1‑94 (MQ=60)
                                                             GCGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAA                                   <  SRR4302545.3112885/94‑1 (MQ=60)
                                                              CGTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAAC                                  >  SRR4302545.2237247/1‑94 (MQ=60)
                                                               GTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACT                                 >  SRR4302545.10272875/1‑94 (MQ=60)
                                                               GTAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACT                                 >  SRR4302545.1400050/1‑94 (MQ=60)
                                                                TAATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTT                                >  SRR4302545.5219892/1‑94 (MQ=60)
                                                                 AATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTG                               <  SRR4302545.7633799/94‑1 (MQ=60)
                                                                  ATGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGC                              <  SRR4302545.9732313/94‑1 (MQ=60)
                                                                   TGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCC                             <  SRR4302545.9481784/94‑1 (MQ=60)
                                                                   TGGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCC                             <  SRR4302545.9481783/94‑1 (MQ=60)
                                                                    GGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCG                            <  SRR4302545.7888326/94‑1 (MQ=60)
                                                                    GGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCG                            <  SRR4302545.7888327/94‑1 (MQ=60)
                                                                    GGTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCG                            <  SRR4302545.6497342/94‑1 (MQ=60)
                                                                     GTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGG                           >  SRR4302545.3802974/1‑94 (MQ=60)
                                                                     GTGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGG                           <  SRR4302545.9606590/94‑1 (MQ=60)
                                                                      TGGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGG                          >  SRR4302545.2957134/1‑94 (MQ=60)
                                                                       GGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGCACTTGCCGGGC                         <  SRR4302545.7052722/94‑1 (MQ=60)
                                                                       GGTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGC                         <  SRR4302545.7786114/94‑1 (MQ=60)
                                                                       GGTGTTTTCGAACAGGCAGGCGCAAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCCCCGCTCATCGCCCGGAACTTGCCGGGC                         <  SRR4302545.6075674/94‑1 (MQ=60)
                                                                        GTGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCG                        <  SRR4302545.6845961/94‑1 (MQ=60)
                                                                         TGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGC                       >  SRR4302545.3089823/1‑94 (MQ=60)
                                                                         TGTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGC                       >  SRR4302545.1848657/1‑94 (MQ=60)
                                                                         TGTTTTCGAACAGGCAGGCGCCAACTTTTCCCATGTCCACGGCTAGGCGATGCCTGCTTCGGCCACCCCTCATCACGCGGAACTTGCCCGGCGC                       >  SRR4302545.1848658/1‑94 (MQ=53)
                                                                          GTTTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCA                      <  SRR4302545.1801050/94‑1 (MQ=60)
                                                                            TTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGT                    >  SRR4302545.155149/1‑94 (MQ=60)
                                                                            TTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGT                    <  SRR4302545.9972623/94‑1 (MQ=60)
                                                                            TTTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGT                    >  SRR4302545.4917985/1‑94 (MQ=60)
                                                                             TTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTT                   <  SRR4302545.9360048/94‑1 (MQ=60)
                                                                             TTCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTT                   <  SRR4302545.1653184/94‑1 (MQ=60)
                                                                              TCGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTT                  <  SRR4302545.4304482/94‑1 (MQ=60)
                                                                               CGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTC                 <  SRR4302545.10477267/94‑1 (MQ=60)
                                                                               CGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTC                 <  SRR4302545.2975958/94‑1 (MQ=60)
                                                                               CGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTC                 >  SRR4302545.1042235/1‑94 (MQ=60)
                                                                               CGAACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTC                 <  SRR4302545.5283898/94‑1 (MQ=60)
                                                                                 AACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAATTTGCCGGGCGCAGTTTCGA               <  SRR4302545.2089934/94‑1 (MQ=60)
                                                                                 AACAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGA               >  SRR4302545.9077221/1‑94 (MQ=60)
                                                                                   CAGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGG             >  SRR4302545.10409036/1‑94 (MQ=60)
                                                                                    AGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGC            <  SRR4302545.303631/94‑1 (MQ=60)
                                                                                    AGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGC            >  SRR4302545.6294520/1‑94 (MQ=60)
                                                                                    AGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGC            >  SRR4302545.8131620/1‑94 (MQ=60)
                                                                                    AGGCAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGC            >  SRR4302545.454970/1‑94 (MQ=60)
                                                                                       CAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGAT         >  SRR4302545.1576951/1‑94 (MQ=60)
                                                                                       CAGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCCGAACTTGCCGGGCGCGGTTTCGAGGCGAG         >  SRR4302545.2060925/1‑94 (MQ=60)
                                                                                        AGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATG        <  SRR4302545.5006994/94‑1 (MQ=60)
                                                                                        AGGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCAGGCGCAGCTTCGCGGCGATG        >  SRR4302545.4012060/1‑94 (MQ=60)
                                                                                         GGCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGG       <  SRR4302545.6120/94‑1 (MQ=60)
                                                                                          GCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGG      >  SRR4302545.3855765/1‑94 (MQ=60)
                                                                                          GCGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCACGTAACTTGCCGGGCGCCGTTTCGGGGCGGTGGG      >  SRR4302545.4111879/1‑94 (MQ=60)
                                                                                           CGCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGGC     >  SRR4302545.6346753/1‑94 (MQ=60)
                                                                                            GCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGGCG    >  SRR4302545.8170402/1‑94 (MQ=60)
                                                                                            GCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGGCG    <  SRR4302545.1175983/94‑1 (MQ=60)
                                                                                            GCCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGGCG    <  SRR4302545.1175982/94‑1 (MQ=60)
                                                                                             CCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGGCGT   >  SRR4302545.1967967/1‑94 (MQ=60)
                                                                                             CCAACTTTTCGCATGTCCAC‑GTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGGCGTT  >  SRR4302545.2930945/1‑94 (MQ=60)
                                                                                             |                                                                                              
CAGAATTTGTCGAAGATAGTTGGCAGCGCGAAGCTGGCGGCGGCGGGCGTAGTCGGGTGTTGCGTAATGGTGGTGTTTTCGAACAGGCAGGCGTCAACTTTTCGCATGTCCACGGTGAGGCGATGCCTGCTTCCGCCACCGCTCATCGCCCGGAACTTGCCGGGCGCAGTTTCGAGGCGATGGGCGTT  >  NC_000913/2551330‑2551517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑