Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,555,797 G→A G301G (GGC→GGT)  b2441 ← ethanolamine ammonia‑lyase, heavy chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,555,7970GA99.2% 904.9 / ‑5.6 242G301G (GGC→GGT) b2441ethanolamine ammonia‑lyase, heavy chain
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (141/99);  total (142/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GCCCAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCC  >  NC_000913/2555706‑2555887
                                                                                           |                                                                                          
gCCCAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGcc                                                                                          >  1:7393005/1‑94 (MQ=255)
   cAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGag                                                                                       >  2:6418367/1‑93 (MQ=255)
   cAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGaa                                                                                       >  1:7504065/1‑94 (MQ=255)
   cAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGaa                                                                                       <  2:7338122/94‑1 (MQ=255)
    aaTAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAg                                                                                      >  1:7756185/1‑94 (MQ=255)
       aaaGCCGACCACTGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTa                                                                                   >  2:1245069/1‑94 (MQ=255)
       aaaGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTa                                                                                   <  2:6982109/94‑1 (MQ=255)
         aGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAgc                                                                                 >  2:4717193/1‑94 (MQ=255)
         aGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAgc                                                                                 >  2:10613384/1‑94 (MQ=255)
          gCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAgcg                                                                                <  1:10447826/94‑1 (MQ=255)
            cGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGcc                                                                              <  2:1883529/94‑1 (MQ=255)
             gACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCa                                                                             <  1:3943760/94‑1 (MQ=255)
               ccACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGc                                                                           <  2:2612786/94‑1 (MQ=255)
               ccACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGc                                                                           >  2:10373014/1‑94 (MQ=255)
               ccACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGc                                                                           >  1:3162049/1‑94 (MQ=255)
               ccACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGc                                                                           >  1:3486545/1‑94 (MQ=255)
               ccACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGc                                                                           <  2:448263/94‑1 (MQ=255)
                cACTGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCg                                                                          <  2:5959602/94‑1 (MQ=255)
                cACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCg                                                                          >  2:1507423/1‑94 (MQ=255)
                cACGGTGGTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTATCGCCAGCg                                                                          >  1:8698683/1‑94 (MQ=255)
                 aCGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCgg                                                                         >  2:4155969/1‑94 (MQ=255)
                  cGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGa                                                                        >  2:305187/1‑94 (MQ=255)
                  cGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGa                                                                        >  1:6230623/1‑94 (MQ=255)
                   ggTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTCGCGCCAGCGGAt                                                                       >  2:6295841/1‑94 (MQ=255)
                   ggTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGAt                                                                       <  2:9721689/94‑1 (MQ=255)
                   ggTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGAt                                                                       >  2:5018279/1‑94 (MQ=255)
                    gtgtTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATa                                                                      <  2:1637523/94‑1 (MQ=255)
                    gtgtTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATa                                                                      >  1:9836854/1‑94 (MQ=255)
                     tgtTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAg                                                                     <  1:4270576/94‑1 (MQ=255)
                        tGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAgcgc                                                                  >  2:1761293/1‑94 (MQ=255)
                        tGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAgcgc                                                                  >  1:8267229/1‑94 (MQ=255)
                        tGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAgcgc                                                                  >  1:487091/1‑94 (MQ=255)
                        tGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAgcgc                                                                  >  1:1280696/1‑94 (MQ=255)
                         gACGATAAACGGATCGTAATGACTCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCa                                                                 <  1:7171840/94‑1 (MQ=255)
                          aCGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCag                                                                >  2:4028281/1‑94 (MQ=255)
                               aaaCGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCt                                                           >  1:1378065/1‑94 (MQ=255)
                               aaaCGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCt                                                           >  1:9581332/1‑94 (MQ=255)
                                aaCGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCtt                                                          <  2:8093799/94‑1 (MQ=255)
                                  cGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGCAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGt                                                        >  1:10610042/1‑94 (MQ=255)
                                  cGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGt                                                        <  1:8023297/94‑1 (MQ=255)
                                  cGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGt                                                        <  1:8465068/94‑1 (MQ=255)
                                   ggATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTc                                                       >  2:8743848/1‑94 (MQ=255)
                                     aTCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCg                                                     <  2:3134451/94‑1 (MQ=255)
                                     aTCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCg                                                     >  2:6114743/1‑94 (MQ=255)
                                     aTCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCg                                                     >  1:4025147/1‑94 (MQ=255)
                                      tCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCgg                                                    <  1:2125372/94‑1 (MQ=255)
                                        gTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGtt                                                  >  2:4557419/1‑94 (MQ=255)
                                          aaTGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTc                                                <  2:4919572/94‑1 (MQ=255)
                                          aaTGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTc                                                >  2:6891533/1‑94 (MQ=255)
                                           aTGACGCGCCAGCCCGTAGTTACGTGCTTCCCTCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCg                                               <  2:8563479/94‑1 (MQ=255)
                                           aTGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCg                                               <  1:10344025/94‑1 (MQ=255)
                                            tGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGa                                              >  1:7178326/1‑94 (MQ=255)
                                               cgcgCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGt                                           >  2:2293946/1‑94 (MQ=255)
                                                gcgcCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTa                                          <  1:6811681/94‑1 (MQ=255)
                                                gcgcCAGCCAGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTa                                          <  1:10079077/94‑1 (MQ=255)
                                                 cgcCAGCCCGTAGTTACGTGCTTCCATCGTTGCCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTag                                         <  1:7419821/94‑1 (MQ=255)
                                                 cgcCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTag                                         >  1:6587319/1‑94 (MQ=255)
                                                 cgcCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTag                                         <  2:5147320/94‑1 (MQ=255)
                                                  gcCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTaga                                        >  2:5062941/1‑94 (MQ=255)
                                                    cAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAgg                                      <  2:7095395/94‑1 (MQ=255)
                                                    cAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAgg                                      <  1:9774640/94‑1 (MQ=255)
                                                    cAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAgg                                      >  1:5013630/1‑94 (MQ=255)
                                                    cAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAgg                                      >  1:1422135/1‑94 (MQ=255)
                                                      gCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCa                                    >  2:5820222/1‑94 (MQ=255)
                                                       cccGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAg                                   <  2:1123143/94‑1 (MQ=255)
                                                       cccGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAg                                   <  2:2177370/94‑1 (MQ=255)
                                                        ccGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGt                                  >  1:564763/1‑94 (MQ=255)
                                                        ccGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGt                                  >  2:8882523/1‑94 (MQ=255)
                                                        ccGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGt                                  <  2:9448294/94‑1 (MQ=255)
                                                        ccGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGt                                  <  2:6602912/94‑1 (MQ=255)
                                                         cGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTCGAGGCCGtt                                 >  2:8016892/1‑94 (MQ=255)
                                                         cGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGtt                                 >  2:8448148/1‑94 (MQ=255)
                                                          gTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGTCAGttt                                >  1:1875269/1‑94 (MQ=255)
                                                          gTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGttt                                >  2:3545848/1‑94 (MQ=255)
                                                            aGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGAAGAGGGCGTTTTc                              >  1:2529511/1‑94 (MQ=255)
                                                             gTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTcc                             >  1:5183542/1‑94 (MQ=255)
                                                             gTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGAAGTTTTcc                             >  1:2386332/1‑94 (MQ=255)
                                                               tACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTcccc                           >  1:4590731/1‑94 (MQ=255)
                                                               tACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTcccc                           >  2:7775895/1‑94 (MQ=255)
                                                                aCGTGCTTCCATCGTTACCTGGTATGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCg                          <  2:1458595/94‑1 (MQ=255)
                                                                 cGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCgg                         >  1:3777242/1‑94 (MQ=255)
                                                                 cGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCgg                         >  1:3108875/1‑94 (MQ=255)
                                                                 cGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCCTGTTCGGTTTCGAAGTAGATGCAGTTTTCGCCgg                         >  1:2728789/1‑94 (MQ=255)
                                                                      ttCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATa                    >  1:5616774/1‑94 (MQ=255)
                                                                        ccATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACg                  <  1:9524383/94‑1 (MQ=255)
                                                                         cATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCAGGCGATACgg                 >  2:2839163/1‑94 (MQ=255)
                                                                          aTCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt                >  1:4355096/1‑94 (MQ=255)
                                                                           tCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGtt               <  2:7641513/94‑1 (MQ=255)
                                                                            cGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTg              >  1:9134543/1‑94 (MQ=255)
                                                                             gTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGa             >  2:111769/1‑94 (MQ=255)
                                                                             gTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGa             >  2:2486930/1‑94 (MQ=255)
                                                                                aCCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGGCTTGTCTGGTTTCGAAGTAGAGGCAGCTTTCCCCGGCGATACGGTTGAACt          >  2:4825184/1‑94 (MQ=255)
                                                                                aCCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACt          >  1:6063253/1‑94 (MQ=255)
                                                                                 ccTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTc         >  1:208249/1‑94 (MQ=255)
                                                                                 ccTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTc         <  2:2340543/94‑1 (MQ=255)
                                                                                  cTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTcc        >  2:4345186/1‑94 (MQ=255)
                                                                                   tGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCcg       <  2:1939653/94‑1 (MQ=255)
                                                                                   tGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCcg       >  1:8194912/1‑94 (MQ=255)
                                                                                       cTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGCACTCCGCGcc   >  1:5890721/1‑94 (MQ=255)
                                                                                       cTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGcc   >  2:3847584/1‑94 (MQ=255)
                                                                                           |                                                                                          
GCCCAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCC  >  NC_000913/2555706‑2555887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCCAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACT  >  NC_000913/2555706‑2555890
                                                                                           |                                                                                             
GCCCAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGCC                                                                                             >  SRR4302545.7601645/1‑94 (MQ=60)
GCCCAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACC                                                                                             >  SRR4302545.4422395/1‑94 (MQ=60)
 CCCAATAAAGCCGACCACGGTGTTGACGATAAACTGATCGTAATGACGCGCCAGCCCGTAGGTACGTGCTTCCATCGTTACCTGGTCTGCACCG                                                                                            >  SRR4302545.7061613/1‑94 (MQ=60)
   CAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAA                                                                                          >  SRR4302545.7716406/1‑94 (MQ=60)
   CAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAA                                                                                          <  SRR4302545.7702858/94‑1 (MQ=60)
   CAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATAACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAA                                                                                          <  SRR4302545.5530746/94‑1 (MQ=60)
    AATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAG                                                                                         >  SRR4302545.7975684/1‑94 (MQ=60)
     ATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGT                                                                                        <  SRR4302545.4999194/94‑1 (MQ=60)
     ATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGT                                                                                        >  SRR4302545.8004496/1‑94 (MQ=60)
       AAAGCGGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTA                                                                                      <  SRR4302545.3127470/94‑1 (MQ=60)
       AAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTA                                                                                      >  SRR4302545.8909144/1‑94 (MQ=60)
       AAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTA                                                                                      <  SRR4302545.1947569/94‑1 (MQ=60)
        AAGCCGACCACGGTGTTGACGATAAACGGATCGTAGTGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAG                                                                                     >  SRR4302545.6868790/1‑94 (MQ=60)
        AAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTCG                                                                                     >  SRR4302545.6686915/1‑94 (MQ=60)
          GCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCG                                                                                   <  SRR4302545.10755743/94‑1 (MQ=60)
           CCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGC                                                                                  >  SRR4302545.4035517/1‑94 (MQ=60)
             GACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCA                                                                                <  SRR4302545.4042264/94‑1 (MQ=60)
             GACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCA                                                                                <  SRR4302545.404535/94‑1 (MQ=60)
             GACCACGGTGTTGACGAGAAACGTATAGTAATGACGCGCCAGCCCGTATTTACGTGCTTCCATCGTTACCTGTTCTGCACCGAAGTTAGCGCCA                                                                                <  SRR4302545.7422855/94‑1 (MQ=60)
               CCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGC                                                                              >  SRR4302545.3572015/1‑94 (MQ=60)
               CCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGC                                                                              >  SRR4302545.3239759/1‑94 (MQ=60)
               CCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGC                                                                              >  SRR4302545.4345187/1‑94 (MQ=60)
               CCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGC                                                                              >  SRR4302545.9872467/1‑94 (MQ=60)
                CACGGTGGTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTATCGCCAGCG                                                                             >  SRR4302545.8950993/1‑94 (MQ=60)
                 ACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGG                                                                            >  SRR4302545.81594/1‑94 (MQ=60)
                 ACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGG                                                                            <  SRR4302545.6153465/94‑1 (MQ=60)
                  CGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGTTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGA                                                                           <  SRR4302545.3385803/94‑1 (MQ=60)
                  CGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGA                                                                           >  SRR4302545.6400400/1‑94 (MQ=60)
                    GTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATA                                                                         <  SRR4302545.10857803/94‑1 (MQ=60)
                    GTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATA                                                                         >  SRR4302545.10129146/1‑94 (MQ=60)
                     TGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAG                                                                        <  SRR4302545.7375175/94‑1 (MQ=60)
                     TGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAG                                                                        <  SRR4302545.7453836/94‑1 (MQ=60)
                     TGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAG                                                                        <  SRR4302545.4377768/94‑1 (MQ=60)
                        TGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGC                                                                     >  SRR4302545.8506508/1‑94 (MQ=60)
                        TGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGC                                                                     >  SRR4302545.497961/1‑94 (MQ=60)
                        TGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGC                                                                     >  SRR4302545.1309241/1‑94 (MQ=60)
                         GACGATAAACGGATCGTAATGACTCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCA                                                                    <  SRR4302545.7371064/94‑1 (MQ=60)
                         GACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCA                                                                    <  SRR4302545.7371063/94‑1 (MQ=60)
                          ACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAG                                                                   <  SRR4302545.1856284/94‑1 (MQ=60)
                          ACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAG                                                                   >  SRR4302545.5049695/1‑94 (MQ=60)
                               AAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCT                                                              >  SRR4302545.1408950/1‑94 (MQ=60)
                               AAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCT                                                              >  SRR4302545.1408951/1‑94 (MQ=60)
                               AAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCT                                                              >  SRR4302545.9863710/1‑94 (MQ=60)
                                AACGGATCGTAATGACGAGCCAGACCGCAGTTACGTGCTTCCATCGTTCCCTGGTCTGCACCGAAGTTAGCCCCAGCGGATAGCGCAGAGCCTT                                                             <  SRR4302545.3597415/94‑1 (MQ=60)
                                  CGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGCAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGT                                                           >  SRR4302545.10922428/1‑94 (MQ=60)
                                  CGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGT                                                           <  SRR4302545.8709706/94‑1 (MQ=60)
                                  CGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGT                                                           <  SRR4302545.8251795/94‑1 (MQ=60)
                                   GGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTC                                                          >  SRR4302545.7792849/1‑94 (MQ=60)
                                   GGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGGTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTC                                                          >  SRR4302545.1479277/1‑94 (MQ=60)
                                     ATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCG                                                        <  SRR4302545.546542/94‑1 (MQ=60)
                                     ATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCG                                                        >  SRR4302545.4125884/1‑94 (MQ=60)
                                      TCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGG                                                       <  SRR4302545.2177530/94‑1 (MQ=60)
                                        GTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTT                                                     <  SRR4302545.7475686/94‑1 (MQ=60)
                                        GTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTT                                                     >  SRR4302545.9188216/1‑94 (MQ=60)
                                         TAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTT                                                    <  SRR4302545.4518857/94‑1 (MQ=60)
                                          AATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTC                                                   <  SRR4302545.1193726/94‑1 (MQ=60)
                                           ATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCG                                                  <  SRR4302545.7643654/94‑1 (MQ=60)
                                           ATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCG                                                  <  SRR4302545.10648937/94‑1 (MQ=60)
                                            TGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGA                                                 >  SRR4302545.7377808/1‑94 (MQ=60)
                                            TGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGA                                                 >  SRR4302545.6216184/1‑94 (MQ=60)
                                             GACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAA                                                >  SRR4302545.9409089/1‑94 (MQ=60)
                                               CGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAACGCAGAGCCTTGTCCGGATTCGAAGA                                              >  SRR4302545.9830362/1‑94 (MQ=60)
                                                GCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTA                                             <  SRR4302545.6998876/94‑1 (MQ=60)
                                                GCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTA                                             <  SRR4302545.6276892/94‑1 (MQ=60)
                                                GCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTA                                             >  SRR4302545.9952122/1‑94 (MQ=60)
                                                GCGCCAGCCAGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTA                                             <  SRR4302545.10377239/94‑1 (MQ=60)
                                                 CGCCAGCCCGTAGTTACGTGCTTCCATCGTTGCCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAG                                            <  SRR4302545.7629579/94‑1 (MQ=60)
                                                 CGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAG                                            >  SRR4302545.2164663/1‑94 (MQ=60)
                                                 CGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAG                                            >  SRR4302545.6767606/1‑94 (MQ=60)
                                                   CCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAG                                          >  SRR4302545.8473168/1‑94 (MQ=60)
                                                    CAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGG                                         >  SRR4302545.5140743/1‑94 (MQ=60)
                                                    CAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGG                                         >  SRR4302545.1454365/1‑94 (MQ=60)
                                                    CAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGG                                         <  SRR4302545.10065320/94‑1 (MQ=60)
                                                     AGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGA                                        >  SRR4302545.1322063/1‑94 (MQ=60)
                                                      GCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCA                                       <  SRR4302545.10075019/94‑1 (MQ=60)
                                                        CCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGGCTTGTCCGGTTTCGAAGTggagggagg                                     >  SRR4302545.2347970/1‑85 (MQ=60)
                                                        CCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGT                                     >  SRR4302545.577459/1‑94 (MQ=60)
                                                        CCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGT                                     <  SRR4302545.10820857/94‑1 (MQ=60)
                                                        CCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGT                                     >  SRR4302545.4966946/1‑94 (MQ=60)
                                                        CCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGT                                     >  SRR4302545.577458/1‑94 (MQ=60)
                                                         CGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTT                                    >  SRR4302545.2156124/1‑94 (MQ=60)
                                                          GTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGTCAGTTT                                   >  SRR4302545.1921343/1‑94 (MQ=60)
                                                          GTAGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTT                                   <  SRR4302545.8469474/94‑1 (MQ=60)
                                                            AGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTC                                 <  SRR4302545.5254288/94‑1 (MQ=60)
                                                            AGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTC                                 >  SRR4302545.321777/1‑94 (MQ=60)
                                                            AGTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGAAGAGGGCGTTTTC                                 >  SRR4302545.2590627/1‑94 (MQ=60)
                                                             GTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCC                                >  SRR4302545.5316994/1‑94 (MQ=60)
                                                             GTTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGAAGTTTTCC                                >  SRR4302545.2444220/1‑94 (MQ=60)
                                                              TTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCC                               <  SRR4302545.2486184/94‑1 (MQ=60)
                                                              TTACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCC                               >  SRR4302545.2708620/1‑94 (MQ=60)
                                                               TACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCC                              >  SRR4302545.4708293/1‑94 (MQ=60)
                                                               TACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCC                              >  SRR4302545.4150469/1‑94 (MQ=60)
                                                               TACGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAAAGCCTTGTCCGGATTAGAAGTAGAGGCAGTTTTCCCC                              >  SRR4302545.5144219/1‑94 (MQ=60)
                                                                 CGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGG                            >  SRR4302545.3870606/1‑94 (MQ=60)
                                                                 CGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGG                            >  SRR4302545.3185474/1‑94 (MQ=60)
                                                                 CGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGG                            <  SRR4302545.733300/94‑1 (MQ=60)
                                                                 CGTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCCTGTTCGGTTTCGAAGTAGATGCAGTTTTCGCCGG                            >  SRR4302545.2794606/1‑94 (MQ=60)
                                                                  GTGCTTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGG                           >  SRR4302545.8912539/1‑94 (MQ=60)
                                                                  GTGCTTCCATCGTTACCGGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGC                           <  SRR4302545.3734081/94‑1 (MQ=60)
                                                                      TTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATA                       <  SRR4302545.9714281/94‑1 (MQ=60)
                                                                      TTCCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATA                       >  SRR4302545.5765957/1‑94 (MQ=60)
                                                                        CCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACG                     <  SRR4302545.9804804/94‑1 (MQ=60)
                                                                        CCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACG                     <  SRR4302545.4005227/94‑1 (MQ=60)
                                                                        CCATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACG                     <  SRR4302545.8592364/94‑1 (MQ=60)
                                                                          CTCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGT                   <  SRR4302545.3325802/94‑1 (MQ=60)
                                                                          ATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGTAGTAGAGGCAGTTTTCCCCGGCGATACGGT                   <  SRR4302545.6710630/94‑1 (MQ=60)
                                                                          ATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGT                   <  SRR4302545.2505756/94‑1 (MQ=60)
                                                                          ATCGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGT                   >  SRR4302545.4465153/1‑94 (MQ=60)
                                                                            CGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTG                 <  SRR4302545.7602061/94‑1 (MQ=60)
                                                                            CGTTACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTG                 >  SRR4302545.9402795/1‑94 (MQ=60)
                                                                                ACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACT             >  SRR4302545.6227807/1‑94 (MQ=60)
                                                                                ACCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACT             >  SRR4302545.3176208/1‑94 (MQ=60)
                                                                                 CCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTC            >  SRR4302545.212573/1‑94 (MQ=60)
                                                                                 CCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTC            <  SRR4302545.5908601/94‑1 (MQ=60)
                                                                                 CCTGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTC            <  SRR4302545.10669808/94‑1 (MQ=60)
                                                                                   TGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCG          >  SRR4302545.8432084/1‑94 (MQ=60)
                                                                                   TGGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCG          >  SRR4302545.8432083/1‑94 (MQ=60)
                                                                                    GGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGC         >  SRR4302545.1360581/1‑94 (MQ=60)
                                                                                    GGTCTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGC         <  SRR4302545.1047431/94‑1 (MQ=60)
                                                                                      TCTACACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATGCGGTTGAACTCCGCGC       <  SRR4302545.1958082/94‑1 (MQ=60)
                                                                                       CTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGCACTCCGCGCC      >  SRR4302545.6049501/1‑94 (MQ=60)
                                                                                       CTGCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCC      >  SRR4302545.4509356/1‑94 (MQ=60)
                                                                                         GCACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCA    <  SRR4302545.6916433/94‑1 (MQ=60)
                                                                                          CACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAC   <  SRR4302545.879224/94‑1 (MQ=60)
                                                                                          CACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAC   >  SRR4302545.4150350/1‑94 (MQ=60)
                                                                                           ACCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACT  <  SRR4302545.5500767/94‑1 (MQ=60)
                                                                                           |                                                                                             
GCCCAATAAAGCCGACCACGGTGTTGACGATAAACGGATCGTAATGACGCGCCAGCCCGTAGTTACGTGCTTCCATCGTTACCTGGTCTGCGCCGAAGTTAGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACT  >  NC_000913/2555706‑2555890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑