Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,606,063 T→C M376T (ATG→ACG)  hyfF → hydrogenase 4 membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,606,0630TC99.3% 1005.3 / ‑3.8 298M376T (ATG→ACG) hyfFhydrogenase 4 membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (157/138);  total (158/140)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 311 total aligned reads displayed.

AACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGA  >  NC_000913/2605972‑2606153
                                                                                           |                                                                                          
aaCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGc                                                                                          >  1:10150412/1‑94 (MQ=255)
 aCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCt                                                                                         <  2:2724008/94‑1 (MQ=255)
 aCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCt                                                                                         <  1:4695227/94‑1 (MQ=255)
  ccACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTc                                                                                        >  2:3535021/1‑94 (MQ=255)
  ccACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTc                                                                                        <  1:10597468/94‑1 (MQ=255)
  ccACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTc                                                                                        >  1:2473080/1‑94 (MQ=255)
     caGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCaaa                                                                                     >  1:5075772/1‑94 (MQ=255)
      cggcTGGCAAAAACGGCGCTGTTCTGCGGTTCCGGCAATGGACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCaaaa                                                                                    <  1:9174229/91‑1 (MQ=255)
      aGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCaaaa                                                                                    >  2:9859786/1‑94 (MQ=255)
      aGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCCGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCacaa                                                                                    >  2:7325540/1‑94 (MQ=255)
        tCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATc                                                                                  <  2:6622556/94‑1 (MQ=255)
          tGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTAGTCTGTGGGACGCTCAAAATCAt                                                                                >  2:1987965/1‑94 (MQ=255)
            gCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAATTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGc                                                                              <  2:7382984/94‑1 (MQ=255)
              aaaaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCa                                                                            >  1:5548274/1‑94 (MQ=255)
              aaaaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCa                                                                            >  2:5645321/1‑94 (MQ=255)
               aaaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAt                                                                           <  1:1768726/94‑1 (MQ=255)
                aaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAtt                                                                          >  2:7578805/1‑94 (MQ=255)
                 aaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAttt                                                                         >  1:7835810/1‑94 (MQ=255)
                   cGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAc                                                                       >  2:6696777/1‑94 (MQ=255)
                     ctgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccg                                                                     >  2:5089262/1‑94 (MQ=255)
                     ctgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccg                                                                     >  1:1187815/1‑94 (MQ=255)
                     ctgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccg                                                                     >  1:10313049/1‑94 (MQ=255)
                     ctgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccg                                                                     <  2:8289908/94‑1 (MQ=255)
                     ctgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccg                                                                     >  1:2557147/1‑94 (MQ=255)
                       gctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccgcc                                                                   <  2:3831939/94‑1 (MQ=255)
                         tgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGt                                                                 <  2:4972857/94‑1 (MQ=255)
                          gTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTg                                                                <  2:1595575/94‑1 (MQ=255)
                          gTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTg                                                                >  1:3188764/1‑94 (MQ=255)
                             cTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTg                                                             >  1:4831644/1‑94 (MQ=255)
                              tGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGt                                                            >  1:7117620/1‑94 (MQ=255)
                              tGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGt                                                            >  1:9840017/1‑94 (MQ=255)
                                 ggTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTg                                                         >  2:2280473/1‑94 (MQ=255)
                                  gTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTgg                                                        >  2:6440519/1‑94 (MQ=255)
                                  gTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTgg                                                        >  2:9161140/1‑94 (MQ=255)
                                   ttCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggc                                                       >  2:5251540/1‑94 (MQ=255)
                                   ttCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggc                                                       >  1:4189460/1‑94 (MQ=255)
                                      cGGCAATGTACGGGTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcggc                                                    <  1:4931712/94‑1 (MQ=255)
                                       ggCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGgcggcg                                                   >  1:3571963/1‑94 (MQ=255)
                                          aaTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTg                                                <  2:10609460/94‑1 (MQ=255)
                                          aaTGTACTGCTAAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGcggcggcg                                                >  1:7488649/1‑92 (MQ=255)
                                           aTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgg                                               >  1:1356006/1‑93 (MQ=255)
                                           aTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgc                                               >  1:10265232/1‑94 (MQ=255)
                                           aTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgc                                               <  2:718853/94‑1 (MQ=255)
                                            tGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGTTCAAAATCATGCCATTTCCCGCCGTGCTGTTTAGCGGCGGTgcg                                              <  2:763101/94‑1 (MQ=255)
                                            tGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcg                                              >  1:1253385/1‑94 (MQ=255)
                                            tGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcg                                              >  1:8047192/1‑94 (MQ=255)
                                             gTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgc                                             >  1:10004255/1‑94 (MQ=255)
                                               aCTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctg                                           <  1:3989176/94‑1 (MQ=255)
                                                cTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctgg                                          >  1:1064004/1‑94 (MQ=255)
                                                cTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctgg                                          >  2:1436875/1‑94 (MQ=255)
                                                 tGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggc                                         >  2:5628532/1‑94 (MQ=255)
                                                  gCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcg                                        <  2:8800336/94‑1 (MQ=255)
                                                  gCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcg                                        <  2:5310009/94‑1 (MQ=255)
                                                   cTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcgc                                       >  2:173745/1‑94 (MQ=255)
                                                    tCAAGTACGGCACGCGCGATCTCAGCGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcgct                                      <  2:10014747/94‑1 (MQ=255)
                                                     cAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgggctggcgctg                                     >  2:7719833/1‑94 (MQ=255)
                                                      aaGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGcgctggcgctgg                                    <  1:5330939/94‑1 (MQ=255)
                                                       aGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCgctggcgctggc                                   <  1:873718/94‑1 (MQ=255)
                                                       aGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCgctggcgctggc                                   >  2:979378/1‑94 (MQ=255)
                                                       aGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCgctggcgctggc                                   >  2:9016903/1‑94 (MQ=255)
                                                         tACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAg                                 <  1:4595913/94‑1 (MQ=255)
                                                         tACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAg                                 <  1:3500724/94‑1 (MQ=255)
                                                          aCGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAgg                                >  2:4086876/1‑94 (MQ=255)
                                                           cGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGCGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAggg                               <  2:9224618/94‑1 (MQ=255)
                                                            ggCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGa                              <  1:3737586/94‑1 (MQ=255)
                                                             gCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAt                             <  1:3365605/94‑1 (MQ=255)
                                                              cACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATg                            >  1:6650876/1‑94 (MQ=255)
                                                              cACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATg                            <  1:10570289/94‑1 (MQ=255)
                                                               aCGCGCGATCTCAACGTCGTCTTGGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgc                           >  2:1937915/1‑94 (MQ=255)
                                                               aCGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgc                           >  1:6284872/1‑94 (MQ=255)
                                                                cgcgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgcc                          <  2:6262751/94‑1 (MQ=255)
                                                                cgcgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgcc                          <  1:1849639/94‑1 (MQ=255)
                                                                cgcgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgcc                          <  1:7589619/94‑1 (MQ=255)
                                                                 gcgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccg                         >  1:2879180/1‑94 (MQ=255)
                                                                 gcgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccg                         <  1:2280855/94‑1 (MQ=255)
                                                                  cgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccgc                        >  2:1092636/1‑94 (MQ=255)
                                                                   gcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccgcc                       <  1:5775579/94‑1 (MQ=255)
                                                                   gcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccgcc                       <  2:8663107/94‑1 (MQ=255)
                                                                    cgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGccc                      >  1:2722153/1‑94 (MQ=255)
                                                                     gATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCt                     <  1:4143796/94‑1 (MQ=255)
                                                                     gATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCt                     <  1:2143717/94‑1 (MQ=255)
                                                                     gATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCt                     >  2:3591545/1‑94 (MQ=255)
                                                                      aTCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGGTGGCAGGGATGACGCCCtt                    >  2:453234/1‑94 (MQ=255)
                                                                      aTCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCtt                    <  1:207003/94‑1 (MQ=255)
                                                                          cAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAAc                <  2:3172279/94‑1 (MQ=255)
                                                                          cAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAAc                >  1:8877342/1‑94 (MQ=255)
                                                                            aCGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAt              <  1:5919672/94‑1 (MQ=255)
                                                                            aCGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAt              <  2:6899800/94‑1 (MQ=255)
                                                                             cgtcgtCTGTGTGATTCTCAAAGTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAACAATGACGCCATTCAACAtt             <  1:9340544/94‑1 (MQ=255)
                                                                             cgtcgtCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtt             <  1:7167550/94‑1 (MQ=255)
                                                                             cgtcgtCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtt             >  1:9029048/1‑94 (MQ=255)
                                                                              gtcgtcTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAttt            >  1:4525091/1‑94 (MQ=255)
                                                                                 gtcTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTc         >  2:1231587/1‑94 (MQ=255)
                                                                                 gtcTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTAGCAGGGATGCCGCCCTTCAACATTTTTc         >  1:6824489/1‑94 (MQ=255)
                                                                                   cTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTcct       <  1:860807/94‑3 (MQ=255)
                                                                                    tgtgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTa      >  2:3973347/1‑94 (MQ=255)
                                                                                     gtgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAg     >  1:6071959/1‑94 (MQ=255)
                                                                                      tgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGc    <  2:2819036/94‑1 (MQ=255)
                                                                                        ggACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAATCTTTTTCTTAGCGa  >  2:4892894/1‑94 (MQ=255)
                                                                                        ggACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGa  >  2:9621478/1‑94 (MQ=255)
                                                                                           |                                                                                          
AACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGA  >  NC_000913/2605972‑2606153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATT  >  NC_000913/2605970‑2606156
                                                                                             |                                                                                             
TAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGAC                                                                                               >  SRR4302545.5042601/1‑94 (MQ=60)
 AAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACG                                                                                              >  SRR4302545.5732026/1‑94 (MQ=60)
 AAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACG                                                                                              <  SRR4302545.9167913/94‑1 (MQ=60)
 AAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACG                                                                                              >  SRR4302545.3085358/1‑94 (MQ=60)
 AAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGGGGGACG                                                                                              >  SRR4302545.3085062/1‑94 (MQ=60)
  AACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGC                                                                                             >  SRR4302545.9091438/1‑94 (MQ=60)
  AACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGC                                                                                             >  SRR4302545.10450545/1‑94 (MQ=60)
   ACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCT                                                                                            <  SRR4302545.4814869/94‑1 (MQ=60)
    CCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTC                                                                                           <  SRR4302545.10909516/94‑1 (MQ=60)
    CCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTC                                                                                           >  SRR4302545.2532925/1‑94 (MQ=60)
    CCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTC                                                                                           >  SRR4302545.3172487/1‑94 (MQ=60)
     CACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCA                                                                                          >  SRR4302545.2533008/1‑94 (MQ=60)
       CAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAA                                                                                        >  SRR4302545.5205122/1‑94 (MQ=60)
       CAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAA                                                                                        <  SRR4302545.4360185/94‑1 (MQ=60)
       CAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAA                                                                                        <  SRR4302545.6847241/94‑1 (MQ=60)
       CAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAA                                                                                        >  SRR4302545.10474536/1‑94 (MQ=60)
        cggCTGGCAAAAACGGCGCTGTTCTGCGGTTCCGGCAATGGACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAA                                                                                       <  SRR4302545.9443346/91‑1 (MQ=60)
        AGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAA                                                                                       >  SRR4302545.10894379/1‑94 (MQ=60)
         GTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAAT                                                                                      <  SRR4302545.156906/94‑1 (MQ=60)
          TCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATC                                                                                     <  SRR4302545.6172514/94‑1 (MQ=60)
           CTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCA                                                                                    >  SRR4302545.3362602/1‑94 (MQ=60)
           CTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCA                                                                                    >  SRR4302545.2168887/1‑94 (MQ=60)
            TGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCAT                                                                                   >  SRR4302545.3697542/1‑94 (MQ=60)
            TGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCAT                                                                                   <  SRR4302545.1946120/94‑1 (MQ=60)
              GCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGC                                                                                 <  SRR4302545.3544946/94‑1 (MQ=60)
              GCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGC                                                                                 >  SRR4302545.1770488/1‑94 (MQ=60)
               CAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCC                                                                                <  SRR4302545.6454352/94‑1 (MQ=60)
               CAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCC                                                                                >  SRR4302545.9079001/1‑94 (MQ=60)
                AAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTTAAGTACGGCCCGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCat                                                                               >  SRR4302545.8295709/1‑92 (MQ=60)
                AAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCA                                                                               >  SRR4302545.5694853/1‑94 (MQ=60)
                AAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCA                                                                               <  SRR4302545.2754594/94‑1 (MQ=60)
                 AAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAT                                                                              <  SRR4302545.5058549/94‑1 (MQ=60)
                 AAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAT                                                                              <  SRR4302545.1811117/94‑1 (MQ=60)
                  AAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATT                                                                             >  SRR4302545.1754218/1‑94 (MQ=60)
                   AACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTT                                                                            >  SRR4302545.8057898/1‑94 (MQ=60)
                      GCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACC                                                                         >  SRR4302545.5791543/1‑94 (MQ=60)
                      GCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACC                                                                         <  SRR4302545.2267481/94‑1 (MQ=60)
                       CTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCG                                                                        >  SRR4302545.2618829/1‑94 (MQ=60)
                       CTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCG                                                                        >  SRR4302545.1214270/1‑94 (MQ=60)
                       CTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCG                                                                        >  SRR4302545.10617133/1‑94 (MQ=60)
                       CTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCC                                                                        >  SRR4302545.4720969/1‑94 (MQ=60)
                        TGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGC                                                                       <  SRR4302545.1378021/94‑1 (MQ=60)
                         GCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCC                                                                      >  SRR4302545.1158513/1‑94 (MQ=60)
                           TGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTGAAAATCATGCCATTTACCGCCGT                                                                    >  SRR4302545.7790824/1‑94 (MQ=60)
                            GTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTCCCGCCGTG                                                                   <  SRR4302545.4267201/94‑1 (MQ=60)
                            GTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTG                                                                   >  SRR4302545.373164/1‑94 (MQ=60)
                            GTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTG                                                                   >  SRR4302545.3266945/1‑94 (MQ=60)
                             TTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGC                                                                  >  SRR4302545.7817896/1‑94 (MQ=60)
                              TCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCT                                                                 <  SRR4302545.9512246/94‑1 (MQ=60)
                              TCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCT                                                                 >  SRR4302545.2511584/1‑94 (MQ=60)
                              TCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCT                                                                 >  SRR4302545.6625490/1‑94 (MQ=60)
                               CTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTG                                                                >  SRR4302545.4954921/1‑94 (MQ=60)
                                TGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGT                                                               >  SRR4302545.7314875/1‑94 (MQ=60)
                                TGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGT                                                               <  SRR4302545.5283827/94‑1 (MQ=60)
                                TGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGT                                                               >  SRR4302545.10132391/1‑94 (MQ=60)
                                TGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGT                                                               <  SRR4302545.1862673/94‑1 (MQ=60)
                                 GCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTT                                                              <  SRR4302545.7495033/94‑1 (MQ=60)
                                 GCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTT                                                              >  SRR4302545.8488621/1‑94 (MQ=60)
                                  CGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCGTTTACCGCCGTGCTGTTT                                                             >  SRR4302545.3506962/1‑94 (MQ=60)
                                  CGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTT                                                             >  SRR4302545.8965517/1‑94 (MQ=60)
                                  CGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATATAACGTCGTGCTGCTT                                                             >  SRR4302545.1308648/1‑94 (MQ=60)
                                   GGTTCCGGCAATGTACTGCTCACGGACGGCCAGAGCGATCTCAACGTCGACTGAGGGACGCTCAAAATCATGCCATTTATCGCCGAGCTGTTTG                                                            >  SRR4302545.5623549/1‑94 (MQ=42)
                                    GTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGG                                                           <  SRR4302545.7365362/94‑1 (MQ=60)
                                    GTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGG                                                           <  SRR4302545.7365361/94‑1 (MQ=60)
                                    GTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGG                                                           <  SRR4302545.4703772/94‑1 (MQ=60)
                                    GTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGG                                                           <  SRR4302545.4784532/94‑1 (MQ=60)
                                     TTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGC                                                          >  SRR4302545.1894161/1‑94 (MQ=60)
                                     TTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGC                                                          >  SRR4302545.4294019/1‑94 (MQ=60)
                                      TCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCG                                                         <  SRR4302545.123554/94‑1 (MQ=60)
                                        CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGC                                                       <  SRR4302545.5056767/94‑1 (MQ=60)
                                        CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGC                                                       >  SRR4302545.4947651/1‑94 (MQ=60)
                                        CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGC                                                       >  SRR4302545.6882372/1‑94 (MQ=60)
                                        CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGC                                                       <  SRR4302545.4667948/94‑1 (MQ=60)
                                        CGGCAATGTACGGGTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGC                                                       <  SRR4302545.5056768/94‑1 (MQ=60)
                                         GGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCG                                                      >  SRR4302545.2862426/1‑94 (MQ=60)
                                         GGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCG                                                      >  SRR4302545.3659190/1‑94 (MQ=60)
                                          GCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGG                                                     <  SRR4302545.3974769/94‑1 (MQ=60)
                                           CAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGG                                                    >  SRR4302545.4965760/1‑94 (MQ=60)
                                            AATGTACTGCTAAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGCG                                                   >  SRR4302545.7700601/1‑94 (MQ=60)
                                             ATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGG                                                  >  SRR4302545.1386315/1‑94 (MQ=60)
                                             ATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGC                                                  <  SRR4302545.10377639/94‑1 (MQ=60)
                                             ATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGC                                                  >  SRR4302545.10568110/1‑94 (MQ=60)
                                              TGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCG                                                 >  SRR4302545.8276938/1‑94 (MQ=60)
                                              TGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCG                                                 >  SRR4302545.1281507/1‑94 (MQ=60)
                                               GTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGC                                                >  SRR4302545.4252698/1‑94 (MQ=60)
                                               GTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGC                                                >  SRR4302545.8944544/1‑94 (MQ=60)
                                               GTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGC                                                >  SRR4302545.10300851/1‑94 (MQ=60)
                                               GTACTGCGCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGC                                                >  SRR4302545.10582327/1‑94 (MQ=60)
                                                TACTGCTCAAGTACGGAACGCGCGATCTCAACGTCGTCTGAGCGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCG                                               >  SRR4302545.3865624/1‑94 (MQ=60)
                                                 ACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTG                                              <  SRR4302545.4088960/94‑1 (MQ=60)
                                                  CTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGG                                             >  SRR4302545.1087184/1‑94 (MQ=60)
                                                  CTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGG                                             <  SRR4302545.5650374/94‑1 (MQ=60)
                                                    GCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCG                                           <  SRR4302545.3589347/94‑1 (MQ=60)
                                                     CTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGC                                          >  SRR4302545.6054182/1‑94 (MQ=60)
                                                      TCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCT                                         <  SRR4302545.5341832/94‑1 (MQ=60)
                                                       CAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTG                                        <  SRR4302545.1098341/94‑1 (MQ=60)
                                                        AAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGG                                       <  SRR4302545.10604009/94‑1 (MQ=60)
                                                        AAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGG                                       <  SRR4302545.5469320/94‑1 (MQ=60)
                                                        AAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGCGCGCTGGCGCTGG                                       >  SRR4302545.2190372/1‑94 (MQ=60)
                                                         AGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC                                      >  SRR4302545.4601933/1‑94 (MQ=60)
                                                         AGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGC                                      <  SRR4302545.893322/94‑1 (MQ=60)
                                                           TACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAG                                    <  SRR4302545.3586579/94‑1 (MQ=60)
                                                           TACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAG                                    <  SRR4302545.4713590/94‑1 (MQ=60)
                                                           TACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAG                                    <  SRR4302545.4654697/94‑1 (MQ=60)
                                                           GACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAG                                    <  SRR4302545.7751054/94‑1 (MQ=60)
                                                            ACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGG                                   <  SRR4302545.10890861/94‑1 (MQ=60)
                                                            ACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGG                                   <  SRR4302545.9244730/94‑1 (MQ=60)
                                                             CGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGG                                  >  SRR4302545.10381862/1‑94 (MQ=60)
                                                              GGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGA                                 <  SRR4302545.3829532/94‑1 (MQ=60)
                                                               GCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAT                                <  SRR4302545.9590192/94‑1 (MQ=60)
                                                               GCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAT                                <  SRR4302545.1228858/94‑1 (MQ=60)
                                                               GCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAT                                <  SRR4302545.3447686/94‑1 (MQ=60)
                                                                CACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATG                               >  SRR4302545.6833241/1‑94 (MQ=60)
                                                                CACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATG                               <  SRR4302545.10881665/94‑1 (MQ=60)
                                                                 ACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGC                              >  SRR4302545.6456224/1‑94 (MQ=60)
                                                                 ACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGC                              >  SRR4302545.4908230/1‑94 (MQ=60)
                                                                  CGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCC                             <  SRR4302545.7804275/94‑1 (MQ=60)
                                                                  CGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCC                             <  SRR4302545.1894797/94‑1 (MQ=60)
                                                                  CGCGCGATCTCAACGGCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCGGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCC                             <  SRR4302545.6327646/94‑1 (MQ=60)
                                                                   GCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG                            >  SRR4302545.2948850/1‑94 (MQ=60)
                                                                   GCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG                            <  SRR4302545.2336341/94‑1 (MQ=60)
                                                                   GCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG                            >  SRR4302545.9370259/1‑94 (MQ=60)
                                                                    CGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGC                           >  SRR4302545.3872739/1‑94 (MQ=60)
                                                                    CGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGC                           >  SRR4302545.7100209/1‑94 (MQ=60)
                                                                    CGCGATCTCAACGTCGTCTGCGGGAGGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGCGCGATGGCGCGGGCAGGGATGCCGC                           >  SRR4302545.7100210/1‑94 (MQ=60)
                                                                     GCGATCTGAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCC                          <  SRR4302545.9383555/94‑1 (MQ=60)
                                                                     GCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCC                          <  SRR4302545.5930830/94‑1 (MQ=60)
                                                                      CGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGGCGCCC                         >  SRR4302545.2088031/1‑94 (MQ=60)
                                                                      CGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCC                         <  SRR4302545.3616863/94‑1 (MQ=60)
                                                                      CGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCC                         >  SRR4302545.8092019/1‑94 (MQ=60)
                                                                      CGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCC                         >  SRR4302545.2787808/1‑94 (MQ=60)
                                                                       GATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCT                        <  SRR4302545.2574362/94‑1 (MQ=60)
                                                                       GATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCT                        <  SRR4302545.2196218/94‑1 (MQ=60)
                                                                       GATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCT                        <  SRR4302545.4247322/94‑1 (MQ=60)
                                                                       GATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCT                        >  SRR4302545.10662434/1‑94 (MQ=60)
                                                                       GATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCT                        <  SRR4302545.1066108/94‑1 (MQ=60)
                                                                       GATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGACGCCGCCCT                        >  SRR4302545.5833978/1‑94 (MQ=60)
                                                                        ATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTT                       >  SRR4302545.3020110/1‑94 (MQ=60)
                                                                        ATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTT                       >  SRR4302545.512861/1‑94 (MQ=60)
                                                                        ATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTT                       <  SRR4302545.211306/94‑1 (MQ=60)
                                                                           TCAACGTCGTCTGTGTGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAA                    <  SRR4302545.801631/94‑1 (MQ=60)
                                                                           TCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAA                    >  SRR4302545.2653876/1‑94 (MQ=60)
                                                                            CAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGGCGCCCTTCAAC                   >  SRR4302545.9712180/1‑94 (MQ=60)
                                                                            CAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAAC                   >  SRR4302545.9135285/1‑94 (MQ=60)
                                                                            CAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAAC                   <  SRR4302545.4147377/94‑1 (MQ=60)
                                                                            CAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAAC                   <  SRR4302545.4166951/94‑1 (MQ=60)
                                                                             AACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGGGCTGGCGCTGGCAGGGATGGTGCCCTTCAACA                  >  SRR4302545.9730489/1‑94 (MQ=60)
                                                                             AACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACA                  >  SRR4302545.9442422/1‑94 (MQ=60)
                                                                             AACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACA                  <  SRR4302545.2957506/94‑1 (MQ=60)
                                                                              ACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAT                 <  SRR4302545.6079342/94‑1 (MQ=60)
                                                                              ACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAT                 <  SRR4302545.6079341/94‑1 (MQ=60)
                                                                              ACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAT                 <  SRR4302545.2445846/94‑1 (MQ=60)
                                                                               CGTCGTCTGTGTGATTCTCAAAGTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAACAATGACGCCATTCAACATT                <  SRR4302545.9614408/94‑1 (MQ=60)
                                                                               CGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATT                <  SRR4302545.7366617/94‑1 (MQ=60)
                                                                               CGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATT                <  SRR4302545.2930950/94‑1 (MQ=60)
                                                                               CGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATT                >  SRR4302545.9292139/1‑94 (MQ=60)
                                                                               CGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATT                >  SRR4302545.8252500/1‑94 (MQ=60)
                                                                               CGTCGTCGGTGGGACGCTCAAAATCATGCCATTTCCCGCCGTGCTGTTGGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGGCCTTCAACATT                <  SRR4302545.7366618/94‑1 (MQ=60)
                                                                                GTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTT               >  SRR4302545.4641005/1‑94 (MQ=60)
                                                                                  CGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTT             <  SRR4302545.5614122/94‑1 (MQ=60)
                                                                                  CGTCTGTGGGACGCTCAAAATCATGCCATTTAACGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTT             <  SRR4302545.9265589/94‑1 (MQ=60)
                                                                                  CGTCTGGGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTT             <  SRR4302545.8019790/94‑1 (MQ=60)
                                                                                   GTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTC            >  SRR4302545.10531014/1‑94 (MQ=60)
                                                                                   GTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTAGCAGGGATGCCGCCCTTCAACATTTTTC            >  SRR4302545.7012028/1‑94 (MQ=60)
                                                                                     CTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCCT          <  SRR4302545.880115/94‑1 (MQ=60)
                                                                                      TGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTA         >  SRR4302545.2326544/1‑94 (MQ=60)
                                                                                      TGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTA         >  SRR4302545.3285279/1‑94 (MQ=60)
                                                                                       GTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAG        <  SRR4302545.10927234/94‑1 (MQ=60)
                                                                                       GTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAG        >  SRR4302545.6236798/1‑94 (MQ=60)
                                                                                       GTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAG        >  SRR4302545.6236799/1‑94 (MQ=60)
                                                                                       GTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAG        <  SRR4302545.10930871/94‑1 (MQ=60)
                                                                                        TGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTATC       <  SRR4302545.6644596/94‑1 (MQ=60)
                                                                                        TGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGC       <  SRR4302545.10135325/94‑1 (MQ=60)
                                                                                         GGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCG      <  SRR4302545.6203527/94‑1 (MQ=60)
                                                                                           GACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAA    >  SRR4302545.9179982/1‑94 (MQ=60)
                                                                                           GACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAA    <  SRR4302545.10058970/94‑1 (MQ=60)
                                                                                            ACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAAT   <  SRR4302545.2721868/94‑1 (MQ=60)
                                                                                             CGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATT  >  SRR4302545.5862188/1‑94 (MQ=60)
                                                                                             CGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATT  <  SRR4302545.10401368/94‑1 (MQ=60)
                                                                                             |                                                                                             
TAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATT  >  NC_000913/2605970‑2606156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑