Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,606,063 T→C M376T (ATG→ACG)  hyfF → hydrogenase 4 membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,606,0630TC100.0% 708.5 / NA 207M376T (ATG→ACG) hyfFhydrogenase 4 membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (106/101);  total (106/101)

Only 100 of 212 total aligned reads displayed.

ACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGC  >  NC_000913/2605973‑2606151
                                                                                          |                                                                                        
aCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGc                                                                                        >  1:1468075/1‑93 (MQ=255)
aCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGc                                                                                        <  1:1967503/93‑1 (MQ=255)
aCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGc                                                                                        >  2:3465850/1‑93 (MQ=255)
    caGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCaa                                                                                    <  2:2191199/93‑1 (MQ=255)
      gTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCaaaa                                                                                  <  2:5765945/93‑1 (MQ=255)
      gTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCaaaa                                                                                  <  1:2886523/93‑1 (MQ=255)
       tCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAAt                                                                                 <  2:2587208/93‑1 (MQ=255)
         tGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCa                                                                               >  1:2777945/1‑93 (MQ=255)
             aaacaCGCTTGTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGcc                                                                           <  1:4272487/93‑1 (MQ=255)
             aaaaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGcc                                                                           >  1:5223637/1‑93 (MQ=255)
               aaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAt                                                                         <  2:72529/93‑1 (MQ=255)
               aaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAt                                                                         >  1:5812456/1‑93 (MQ=255)
               aaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAt                                                                         <  1:1686010/93‑1 (MQ=255)
                aaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAtt                                                                        >  2:3999584/1‑93 (MQ=255)
                aaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAtt                                                                        <  2:2441605/93‑1 (MQ=255)
                 aCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGGACGGGACGCTCGATCTCGACGTCGTCTGTGGGACGCTCAAAATCAGGCCAttt                                                                       >  2:3086078/1‑93 (MQ=255)
                  cGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTa                                                                      <  1:4864419/93‑1 (MQ=255)
                   gctgctGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAc                                                                     <  2:2061409/93‑1 (MQ=255)
                    ctgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAcc                                                                    <  2:4707844/93‑1 (MQ=255)
                     tgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccg                                                                   >  2:4774372/1‑93 (MQ=255)
                      gctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccgc                                                                  >  1:5385251/1‑93 (MQ=255)
                       ctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccgcc                                                                 >  1:649461/1‑93 (MQ=255)
                       ctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAccgcc                                                                 <  1:4082150/93‑1 (MQ=255)
                         gTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGt                                                               >  2:1512493/1‑93 (MQ=255)
                         gTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGt                                                               >  1:2511209/1‑93 (MQ=255)
                          ttCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTg                                                              >  1:6234447/1‑93 (MQ=255)
                           tCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGc                                                             <  1:936411/93‑1 (MQ=255)
                           tCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGCACGCTCAAAATCATGCCATTTGCCGCCGTGc                                                             <  2:3838790/93‑1 (MQ=255)
                            cTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCt                                                            <  1:1296922/93‑1 (MQ=255)
                             tGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTg                                                           >  1:1805465/1‑93 (MQ=255)
                             tGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTg                                                           >  1:6061300/1‑93 (MQ=255)
                               cGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGtt                                                         >  2:1443791/1‑93 (MQ=255)
                                ggTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGttt                                                        <  1:3808677/93‑1 (MQ=255)
                                ggTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGttt                                                        >  1:5992829/1‑93 (MQ=255)
                                ggTGCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGttt                                                        <  2:2533085/93‑1 (MQ=255)
                                 gTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTg                                                       >  1:6400717/1‑93 (MQ=255)
                                  ttCCGGCAATGTACTGCTCAAGTCCGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTgg                                                      <  1:1045538/93‑1 (MQ=255)
                                  ttCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTgg                                                      >  2:4459293/1‑93 (MQ=255)
                                     cGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcgg                                                   >  1:4573678/1‑93 (MQ=255)
                                     cGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcgg                                                   <  1:3041086/93‑1 (MQ=255)
                                      ggCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcggg                                                  >  1:4449270/1‑92 (MQ=255)
                                       gCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGgcggcg                                                 >  2:172077/1‑93 (MQ=255)
                                       gCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGgcggcg                                                 <  1:3553851/93‑1 (MQ=255)
                                       gCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGgcggcg                                                 <  1:2272929/93‑1 (MQ=255)
                                         aaTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGt                                               <  1:3306073/93‑1 (MQ=255)
                                         aaTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGt                                               <  2:3534878/93‑1 (MQ=255)
                                         aaTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGt                                               <  1:5635746/93‑1 (MQ=255)
                                          aTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTg                                              >  1:3258089/1‑93 (MQ=255)
                                          aTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGGCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTg                                              <  1:205164/93‑1 (MQ=255)
                                             tACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgc                                           >  2:2952475/1‑93 (MQ=255)
                                             tACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgc                                           <  2:593484/93‑1 (MQ=255)
                                             tACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgc                                           >  2:2255626/1‑93 (MQ=255)
                                              aCTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgct                                          <  2:2327032/93‑1 (MQ=255)
                                               cTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctg                                         <  2:4569826/93‑1 (MQ=255)
                                                tGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctgg                                        <  2:1302785/93‑1 (MQ=255)
                                                 gCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggc                                       >  2:5672445/1‑93 (MQ=255)
                                                   tCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcgc                                     >  1:6145172/1‑93 (MQ=255)
                                                   tCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcgc                                     <  1:2339954/93‑1 (MQ=255)
                                                   tCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcgc                                     <  2:4347550/93‑1 (MQ=255)
                                                   tCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcgc                                     <  1:4910475/93‑1 (MQ=255)
                                                    cAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcgct                                    <  2:2351140/93‑1 (MQ=255)
                                                      aGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGcgctggcgctgg                                  >  1:5004683/1‑93 (MQ=255)
                                                       gTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCgctggcgctggc                                 >  2:2927296/1‑93 (MQ=255)
                                                         aCGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAg                               <  1:800374/93‑1 (MQ=255)
                                                           agCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAggg                             >  1:3936205/2‑93 (MQ=255)
                                                            gCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGa                            >  2:5549999/1‑93 (MQ=255)
                                                            gCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGa                            <  2:6398882/93‑1 (MQ=255)
                                                             cACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAt                           <  2:4116368/93‑1 (MQ=255)
                                                               cgcgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCGTTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgc                         <  1:3700066/93‑1 (MQ=255)
                                                               cgcgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgc                         <  1:3364575/93‑1 (MQ=255)
                                                                gcgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgcc                        <  1:1666405/93‑1 (MQ=255)
                                                                 cgcgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccg                       >  2:6062127/1‑93 (MQ=255)
                                                                   cgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGTAGGGATgccgcc                     >  1:5339636/1‑93 (MQ=255)
                                                                   cgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccgcc                     >  1:6438659/1‑93 (MQ=255)
                                                                   cgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccgcc                     >  1:5102321/1‑93 (MQ=255)
                                                                   cgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccgcc                     <  2:6355365/93‑1 (MQ=255)
                                                                   cgATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccgcc                     >  2:2640516/1‑93 (MQ=255)
                                                                    gATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGccc                    <  1:4032417/93‑1 (MQ=255)
                                                                     aTCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCt                   <  1:909541/93‑1 (MQ=255)
                                                                      tctcAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCtt                  >  2:1862202/1‑93 (MQ=255)
                                                                      tctcAACGTCGTCTGTGGGACGCTCAAAAGCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCtt                  <  1:3625928/93‑1 (MQ=255)
                                                                        tcAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCa                >  2:518039/1‑93 (MQ=255)
                                                                        tcAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCa                <  2:1055244/93‑1 (MQ=255)
                                                                        tcAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCa                >  1:5303589/1‑93 (MQ=255)
                                                                         cAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCaa               <  1:4731265/93‑1 (MQ=255)
                                                                         cAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCaa               >  2:1018009/1‑93 (MQ=255)
                                                                           aCGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACa             >  2:4142769/1‑93 (MQ=255)
                                                                             gtcgtcTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtt           <  2:4247506/93‑1 (MQ=255)
                                                                              tcgtcTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAttt          >  2:5487697/1‑93 (MQ=255)
                                                                              tcgtcTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAttt          >  2:2905423/1‑93 (MQ=255)
                                                                               cgtcTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtttt         <  1:5186997/93‑1 (MQ=255)
                                                                                gtcTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAttttt        <  2:5388544/93‑1 (MQ=255)
                                                                                  cTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCt      <  2:3785819/93‑1 (MQ=255)
                                                                                   tgtgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCtt     >  1:638033/1‑93 (MQ=255)
                                                                                   tgtgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCtt     >  2:6279784/1‑93 (MQ=255)
                                                                                    gtgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTa    <  1:1781730/93‑1 (MQ=255)
                                                                                    gtgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTa    >  2:4918969/1‑93 (MQ=255)
                                                                                    gtgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTa    <  2:2762650/93‑1 (MQ=255)
                                                                                     tgGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAg   <  1:2152981/93‑1 (MQ=255)
                                                                                      gggACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGc  <  1:3130511/93‑1 (MQ=255)
                                                                                          |                                                                                        
ACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGC  >  NC_000913/2605973‑2606151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATT  >  NC_000913/2605971‑2606156
                                                                                            |                                                                                             
AAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACG                                                                                              >  SRR4302537.2377416/1‑94 (MQ=60)
 AACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGC                                                                                             >  SRR4302537.1478285/1‑94 (MQ=60)
 AACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGC                                                                                             >  SRR4302537.1799187/1‑94 (MQ=60)
  ACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCT                                                                                            <  SRR4302537.1981184/94‑1 (MQ=60)
        GTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAAT                                                                                      <  SRR4302537.2906783/94‑1 (MQ=60)
        GTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAAT                                                                                      >  SRR4302537.2272285/1‑94 (MQ=60)
          CTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCA                                                                                    >  SRR4302537.2797369/1‑94 (MQ=60)
              CAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCC                                                                                >  SRR4302537.5259562/1‑94 (MQ=60)
              CAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCC                                                                                >  SRR4302537.5816738/1‑94 (MQ=60)
              CAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCC                                                                                >  SRR4302537.6081862/1‑94 (MQ=60)
               AAACACGCTTGTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCA                                                                               <  SRR4302537.4302175/94‑1 (MQ=60)
                AAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAT                                                                              >  SRR4302537.5454709/1‑94 (MQ=60)
                AAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCAT                                                                              >  SRR4302537.5852301/1‑94 (MQ=60)
                 AAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATT                                                                             <  SRR4302537.1697814/94‑1 (MQ=60)
                  AACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTT                                                                            >  SRR4302537.883783/1‑94 (MQ=60)
                   ACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTA                                                                           >  SRR4302537.6520210/1‑94 (MQ=60)
                    CGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTAC                                                                          <  SRR4302537.4897983/94‑1 (MQ=60)
                       TGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGC                                                                       >  SRR4302537.5422067/1‑94 (MQ=60)
                        GCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCC                                                                      >  SRR4302537.653888/1‑94 (MQ=60)
                         CTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCG                                                                     <  SRR4302537.6461672/94‑1 (MQ=60)
                         CTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCG                                                                     <  SRR4302537.4110649/94‑1 (MQ=60)
                          TGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGT                                                                    >  SRR4302537.2528868/1‑94 (MQ=60)
                           GTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTG                                                                   >  SRR4302537.6277328/1‑94 (MQ=60)
                            TTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGC                                                                  >  SRR4302537.2066483/1‑94 (MQ=60)
                             TCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCT                                                                 >  SRR4302537.6141128/1‑94 (MQ=60)
                             TCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCT                                                                 <  SRR4302537.942862/94‑1 (MQ=60)
                              CTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTG                                                                >  SRR4302537.6102945/1‑94 (MQ=60)
                              CTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTG                                                                >  SRR4302537.1818061/1‑94 (MQ=60)
                              CTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTG                                                                <  SRR4302537.1305939/94‑1 (MQ=60)
                               TGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGT                                                               >  SRR4302537.4940540/1‑94 (MQ=60)
                               TGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGT                                                               >  SRR4302537.335022/1‑94 (MQ=60)
                                GCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCGAAATCATGCCATTTACCGCCGTGCTGTT                                                              >  SRR4302537.3600265/1‑94 (MQ=60)
                                GCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTT                                                              <  SRR4302537.4887715/94‑1 (MQ=60)
                                GCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTT                                                              <  SRR4302537.4642208/94‑1 (MQ=60)
                                 CGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTT                                                             >  SRR4302537.6033987/1‑94 (MQ=60)
                                  GGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTG                                                            >  SRR4302537.6444292/1‑94 (MQ=60)
                                  GGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTG                                                            <  SRR4302537.4047371/94‑1 (MQ=60)
                                  GGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTG                                                            <  SRR4302537.3835381/94‑1 (MQ=60)
                                    TTCCGGCAATGTACTGCTCAAGTCCGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGC                                                          <  SRR4302537.1052754/94‑1 (MQ=60)
                                      CCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGG                                                        >  SRR4302537.4605333/1‑94 (MQ=60)
                                       CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGG                                                       >  SRR4302537.4479997/1‑94 (MQ=60)
                                       CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGC                                                       <  SRR4302537.3062277/94‑1 (MQ=60)
                                       CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGC                                                       <  SRR4302537.5374598/94‑1 (MQ=60)
                                        GGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCG                                                      >  SRR4302537.986322/1‑94 (MQ=60)
                                         GCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGG                                                     <  SRR4302537.2288980/94‑1 (MQ=60)
                                         GCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGG                                                     <  SRR4302537.3578941/94‑1 (MQ=60)
                                         GCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGG                                                     >  SRR4302537.4058228/1‑94 (MQ=60)
                                           AATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTG                                                   <  SRR4302537.5674314/94‑1 (MQ=60)
                                           AATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTG                                                   <  SRR4302537.31765/94‑1 (MQ=60)
                                           AATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTG                                                   >  SRR4302537.3281059/1‑94 (MQ=60)
                                           AATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTG                                                   <  SRR4302537.3329444/94‑1 (MQ=60)
                                            ATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGC                                                  >  SRR4302537.2861475/1‑94 (MQ=60)
                                            ATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGGCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGC                                                  <  SRR4302537.206636/94‑1 (MQ=60)
                                               TACTGCTCAAGTATGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCT                                               >  SRR4302537.665566/1‑94 (MQ=60)
                                               TACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCT                                               >  SRR4302537.941689/1‑94 (MQ=60)
                                               TACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCT                                               >  SRR4302537.5247448/1‑94 (MQ=60)
                                                ACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTG                                              >  SRR4302537.1681814/1‑94 (MQ=60)
                                                ACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTG                                              <  SRR4302537.5027210/94‑1 (MQ=60)
                                                 CTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGG                                             >  SRR4302537.3316762/1‑94 (MQ=60)
                                                  TGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGC                                            <  SRR4302537.5927676/94‑1 (MQ=60)
                                                    CTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGC                                          >  SRR4302537.6187435/1‑94 (MQ=60)
                                                     TCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCT                                         <  SRR4302537.2356444/94‑1 (MQ=60)
                                                     TCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCT                                         <  SRR4302537.4944370/94‑1 (MQ=60)
                                                       AAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGG                                       >  SRR4302537.5039152/1‑94 (MQ=60)
                                                           ACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGG                                   <  SRR4302537.5916303/94‑1 (MQ=60)
                                                           ACGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGG                                   <  SRR4302537.805874/94‑1 (MQ=60)
                                                            CGGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGG                                  >  SRR4302537.509310/1‑94 (MQ=60)
                                                            CAGCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGG                                  >  SRR4302537.3963716/1‑94 (MQ=60)
                                                              GCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAT                                <  SRR4302537.4085038/94‑1 (MQ=60)
                                                              GCACGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAT                                <  SRR4302537.393497/94‑1 (MQ=60)
                                                                 CGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCGTTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCC                             <  SRR4302537.3726094/94‑1 (MQ=60)
                                                                 CGCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCC                             <  SRR4302537.3388405/94‑1 (MQ=60)
                                                                  GCGCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG                            <  SRR4302537.1678080/94‑1 (MQ=60)
                                                                    GCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGTAGGGATGCCGCC                          >  SRR4302537.5376237/1‑94 (MQ=60)
                                                                    GCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCC                          >  SRR4302537.6482368/1‑94 (MQ=60)
                                                                    GCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCC                          >  SRR4302537.5137483/1‑94 (MQ=60)
                                                                    GCGATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCC                          <  SRR4302537.342045/94‑1 (MQ=60)
                                                                      GATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCT                        <  SRR4302537.3114787/94‑1 (MQ=60)
                                                                      GATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCT                        <  SRR4302537.4060637/94‑1 (MQ=60)
                                                                       ATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTT                       <  SRR4302537.6320317/94‑1 (MQ=60)
                                                                       ATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTT                       <  SRR4302537.5320013/94‑1 (MQ=60)
                                                                       ATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTT                       <  SRR4302537.915821/94‑1 (MQ=60)
                                                                       ATCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTT                       <  SRR4302537.2343752/94‑1 (MQ=60)
                                                                        TCTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTC                      >  SRR4302537.2017554/1‑94 (MQ=60)
                                                                        TCTCAACGTCGTCTGTGGGACGCTCAAAAGCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTC                      <  SRR4302537.3651488/94‑1 (MQ=60)
                                                                         CTCGACGTCGTCTGTGGGTCGCTCAAAATCATGCCATTTTCCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCA                     <  SRR4302537.643463/94‑1 (MQ=60)
                                                                         CTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCA                     >  SRR4302537.5340045/1‑94 (MQ=60)
                                                                         CTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCA                     >  SRR4302537.2444825/1‑94 (MQ=60)
                                                                         CTCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCA                     >  SRR4302537.4505693/1‑94 (MQ=60)
                                                                          TCAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAA                    >  SRR4302537.5157582/1‑94 (MQ=60)
                                                                           CAACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAAC                   <  SRR4302537.4763957/94‑1 (MQ=60)
                                                                            AACGTCGTGTGTGGGACGCGCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACA                  <  SRR4302537.3169642/94‑1 (MQ=60)
                                                                            AACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACA                  >  SRR4302537.1851026/1‑94 (MQ=60)
                                                                            AACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACA                  >  SRR4302537.625458/1‑94 (MQ=60)
                                                                             ACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAT                 <  SRR4302537.4401357/94‑1 (MQ=60)
                                                                             ACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAT                 <  SRR4302537.1762943/94‑1 (MQ=60)
                                                                             ACGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAT                 <  SRR4302537.2917979/94‑1 (MQ=60)
                                                                              CGTCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATT                <  SRR4302537.2134237/94‑1 (MQ=60)
                                                                                TCGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTT              >  SRR4302537.140521/1‑94 (MQ=60)
                                                                                 CGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTT             <  SRR4302537.5222672/94‑1 (MQ=60)
                                                                                 CGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTT             <  SRR4302537.4261443/94‑1 (MQ=60)
                                                                                 CGTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTT             <  SRR4302537.1574887/94‑1 (MQ=60)
                                                                                  GTCTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTC            <  SRR4302537.261759/94‑1 (MQ=60)
                                                                                    CTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTT          >  SRR4302537.3693841/1‑94 (MQ=60)
                                                                                    CTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTT          >  SRR4302537.642377/1‑94 (MQ=60)
                                                                                    CTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTT          <  SRR4302537.3774781/94‑1 (MQ=60)
                                                                                    CTGTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTT          >  SRR4302537.5392661/1‑94 (MQ=60)
                                                                                      GTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAG        <  SRR4302537.1794147/94‑1 (MQ=60)
                                                                                      GTGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAG        <  SRR4302537.733310/94‑1 (MQ=60)
                                                                                       TGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGC       <  SRR4302537.5090559/94‑1 (MQ=60)
                                                                                       TGGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGC       <  SRR4302537.2168159/94‑1 (MQ=60)
                                                                                        GGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCG      <  SRR4302537.1109307/94‑1 (MQ=60)
                                                                                        GGGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCG      <  SRR4302537.3152459/94‑1 (MQ=60)
                                                                                         GGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGA     >  SRR4302537.5429838/1‑94 (MQ=60)
                                                                                         GGACGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGA     >  SRR4302537.5510997/1‑94 (MQ=60)
                                                                                            CGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATT  <  SRR4302537.5039023/94‑1 (MQ=60)
                                                                                            |                                                                                             
AAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATT  >  NC_000913/2605971‑2606156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑