Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,737,047 T→C H348R (CAC→CGC)  tyrA ← chorismate mutase‑T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,737,0470TC99.4% 1193.1 / ‑3.4 352H348R (CAC→CGC) tyrAchorismate mutase‑T
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (158/191);  total (161/191)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.74e-01

Only 100 of 362 total aligned reads displayed.

GGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTT  >  NC_000913/2736956‑2737137
                                                                                           |                                                                                          
ggCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAgcgc                                                                                          <  2:10366315/94‑1 (MQ=255)
 gCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCt                                                                                         <  2:636664/94‑1 (MQ=255)
 gCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCt                                                                                         <  1:10342143/94‑1 (MQ=255)
 gCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCt                                                                                         >  2:5468937/1‑94 (MQ=255)
 gCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCt                                                                                         >  2:409133/1‑94 (MQ=255)
  cACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTc                                                                                        >  1:8314301/1‑94 (MQ=255)
   aCTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTcc                                                                                       >  1:2967724/1‑94 (MQ=255)
    cTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTATTCGCCGAACCAGCGCTCCa                                                                                      <  2:305973/94‑1 (MQ=255)
    cTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTccc                                                                                      >  1:3429943/1‑93 (MQ=255)
    cTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCa                                                                                      >  2:6691390/1‑94 (MQ=255)
    cTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCa                                                                                      >  2:2107167/1‑94 (MQ=255)
    cTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCa                                                                                      >  1:8991159/1‑94 (MQ=255)
    cTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCa                                                                                      >  1:10545803/1‑94 (MQ=255)
    cTGGAGTAGAACGGGCGATTGGCAAGCGCCTGACGCAATAACACGCGGCTGGCACGCTGAAAACGCGGTGCGGAATCGCCGAACCAgcgcgtca                                                                                      >  2:9415786/1‑90 (MQ=255)
     tGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCAc                                                                                     >  2:5694062/1‑94 (MQ=255)
      ggATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCAcc                                                                                    <  1:2627735/94‑1 (MQ=255)
      ggATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCAcc                                                                                    >  1:7794446/1‑94 (MQ=255)
       gATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAAGCAGCGCTCCACCt                                                                                   >  1:4152505/1‑94 (MQ=255)
       gATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCt                                                                                   <  2:877941/94‑1 (MQ=255)
       gATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCt                                                                                   >  1:6006689/1‑94 (MQ=255)
        attattACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCtt                                                                                  <  1:1469854/94‑1 (MQ=255)
        attattACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCtt                                                                                  <  1:6747480/94‑1 (MQ=255)
        attattACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCtt                                                                                  >  1:6210767/1‑94 (MQ=255)
           attaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCg                                                                               <  1:5549692/94‑1 (MQ=255)
              aCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGaa                                                                            >  2:7314189/1‑94 (MQ=255)
              aCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGaa                                                                            >  2:1669331/1‑94 (MQ=255)
              aCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGaa                                                                            <  1:487512/94‑1 (MQ=255)
                tGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAAc                                                                          <  2:10541795/94‑1 (MQ=255)
                tGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAAc                                                                          <  2:7819553/94‑1 (MQ=255)
                tGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAAc                                                                          <  2:2229089/94‑1 (MQ=255)
                tGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAAc                                                                          <  2:8563283/94‑1 (MQ=255)
                  gCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTg                                                                        <  2:5909015/94‑1 (MQ=255)
                    gATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTc                                                                      >  2:8892032/1‑94 (MQ=255)
                    gATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAAGCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTc                                                                      >  2:6526338/1‑94 (MQ=255)
                       tttCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAAt                                                                   >  2:34426/3‑94 (MQ=255)
                       tGTCAGTCGCCTGACGCAATAACACGCTGCATTGCCTCTGAAAACGCTGTGCGGAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAAt                                                                   <  2:4202617/94‑1 (MQ=255)
                        gTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATa                                                                  <  2:8396557/94‑1 (MQ=255)
                         tCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATaa                                                                 <  2:8110427/94‑1 (MQ=255)
                          cATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATaaa                                                                >  2:1577987/1‑94 (MQ=255)
                           aTTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAAc                                                               <  1:4651465/94‑1 (MQ=255)
                            ttCGCCTGACGCAATAACACGGGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACg                                                              <  2:4755325/94‑1 (MQ=255)
                            ttCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACg                                                              <  1:2931881/94‑1 (MQ=255)
                            ttCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACg                                                              <  2:9527263/94‑1 (MQ=255)
                              cGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGcc                                                            >  2:8292247/1‑94 (MQ=255)
                              cGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGcc                                                            <  1:1233594/94‑1 (MQ=255)
                              cGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGcc                                                            >  1:9752431/1‑94 (MQ=255)
                               gCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCt                                                           <  1:5750086/94‑1 (MQ=255)
                                ccTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTg                                                          >  2:9776531/1‑94 (MQ=255)
                                 cTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGc                                                         <  1:9527868/94‑1 (MQ=255)
                                  tGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCt                                                        <  1:9628433/94‑1 (MQ=255)
                                   gACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCtt                                                       >  1:3077943/1‑94 (MQ=255)
                                     cGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTAt                                                     <  1:3117025/94‑1 (MQ=255)
                                     cGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTAt                                                     <  2:3108542/94‑1 (MQ=255)
                                       cAATAATGCGGGGTTTTCAATCGGAATACCCTGTGCGTAATCGCCTAACCAGCGCTCCACTTTGGGGAAACTGTCAATAAACGCCTGCTTATCg                                                   <  1:1802130/94‑1 (MQ=255)
                                       cAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTTCGGAAACTGTCAATAAACGCCTGCTTATCg                                                   >  2:7545746/1‑94 (MQ=255)
                                         aTAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGcc                                                 >  1:7169496/1‑94 (MQ=255)
                                          tAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGccc                                                <  2:7694956/94‑1 (MQ=255)
                                           aaCACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCt                                               <  1:8382173/94‑1 (MQ=255)
                                           aaCACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCt                                               <  1:5117059/94‑1 (MQ=255)
                                           aaCACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCt                                               <  1:3211686/94‑1 (MQ=255)
                                            acacGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTg                                              <  1:7102560/94‑1 (MQ=255)
                                            acacGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTg                                              >  2:2426431/1‑94 (MQ=255)
                                             cacGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGc                                             >  2:1777959/1‑94 (MQ=255)
                                              acGCGGCTTTCACTCTGAAAACTCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCt                                            <  2:3004214/94‑1 (MQ=255)
                                              acGCGGCTTTCACTCTGAAAACGCTTTGCGTAATCGGCGAGCCAGCGCTCCACCTTGCGGAAACTGTCAATAATCGCCTGCTTATCGCCCTGCt                                            <  2:1891640/94‑1 (MQ=255)
                                               cgcgGCTTTCACTCTGAAAACGCTGTGCATAATCGACGAAACCGCGCTCCACCTTGCGGAAACTGTCTATCAACGCCTGCTTATCGCCCTGcac                                           <  1:8080716/94‑3 (MQ=255)
                                                 cgGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCa                                         <  2:7288509/94‑1 (MQ=255)
                                                  ggCTTTCACTATGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAg                                        >  1:1841428/1‑94 (MQ=255)
                                                    cTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCa                                      <  2:6753771/94‑1 (MQ=255)
                                                       tCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACt                                   <  1:1625818/94‑1 (MQ=255)
                                                         aCTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCa                                 <  1:8637082/94‑1 (MQ=255)
                                                          ctctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCaa                                >  2:5275534/1‑94 (MQ=255)
                                                           tctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAAAGCCTGCTTATAGCCCTGCTCCAGCAACTCAAt                               >  2:460728/1‑94 (MQ=255)
                                                            ctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtc                              <  1:10125225/94‑1 (MQ=255)
                                                            ctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtc                              >  1:1959161/1‑94 (MQ=255)
                                                            ctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtc                              <  1:2375039/94‑1 (MQ=255)
                                                             tGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcg                             >  2:1425918/1‑94 (MQ=255)
                                                              gAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgc                            <  1:3513197/94‑1 (MQ=255)
                                                                aaaCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcct                          >  2:3662510/1‑94 (MQ=255)
                                                                aaaCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcct                          <  1:2907864/94‑1 (MQ=255)
                                                                aaaCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcct                          >  1:9368092/1‑94 (MQ=255)
                                                                aaaCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAgcgcct                          <  1:10069779/94‑1 (MQ=255)
                                                                 aaCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcctc                         <  2:7137774/94‑1 (MQ=255)
                                                                  aCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcctcg                        >  2:8025850/1‑94 (MQ=255)
                                                                  aCGCTGAGCGTAGTCGCCGACCCAGCGCTCCACCTTGCGGAAACTGCCATTAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcctcg                        <  1:9249902/94‑1 (MQ=255)
                                                                   cGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcctcgc                       >  2:9199030/1‑94 (MQ=255)
                                                                      tgtgCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGa                    >  2:422484/1‑94 (MQ=255)
                                                                       gtgCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGaa                   <  1:682224/94‑1 (MQ=255)
                                                                       gtgCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGaa                   >  1:3955560/1‑94 (MQ=255)
                                                                           gTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGc               >  1:5230781/1‑94 (MQ=255)
                                                                           gTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGc               >  2:2754211/1‑94 (MQ=255)
                                                                                 gCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAg         >  2:119343/1‑94 (MQ=255)
                                                                                  ccGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGt        >  2:6175959/1‑94 (MQ=255)
                                                                                   cGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTa       <  1:7699222/94‑1 (MQ=255)
                                                                                   cGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTa       >  2:5148282/1‑94 (MQ=255)
                                                                                    gAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTaa      >  1:7230039/1‑94 (MQ=255)
                                                                                    gAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTaa      <  1:9614466/94‑1 (MQ=255)
                                                                                    gAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTaa      <  1:6572232/94‑1 (MQ=255)
                                                                                    gAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTaa      <  1:9939342/94‑1 (MQ=255)
                                                                                     aaCCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAAc     <  2:1021052/94‑1 (MQ=255)
                                                                                           |                                                                                          
GGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTT  >  NC_000913/2736956‑2737137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 204 total aligned reads displayed.
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  >  NC_000913/2736954‑2737140
                                                                                             |                                                                                             
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGC                                                                                               <  SRR4302545.845004/94‑1 (MQ=60)
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGC                                                                                               <  SRR4302545.10273119/94‑1 (MQ=60)
CCGGCACTGGATGAGTACTTGCGATTTTAATTCGCCTGAAGCAATAACACGCGGCGTTCACTCTGAAAACGCTTTGCGTAATCGCCGAACCAGC                                                                                               <  SRR4302545.2820909/94‑1 (MQ=60)
 CGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCG                                                                                              <  SRR4302545.1748402/94‑1 (MQ=60)
 CGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCG                                                                                              <  SRR4302545.1952643/94‑1 (MQ=60)
 CGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCG                                                                                              <  SRR4302545.4079678/94‑1 (MQ=60)
 CGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCG                                                                                              >  SRR4302545.10194741/1‑94 (MQ=60)
 CGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCG                                                                                              >  SRR4302545.8948605/1‑94 (MQ=60)
   GCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCT                                                                                            <  SRR4302545.5148990/94‑1 (MQ=60)
   GCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCT                                                                                            <  SRR4302545.10647004/94‑1 (MQ=60)
    CACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTC                                                                                           >  SRR4302545.8555235/1‑94 (MQ=60)
    CACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTC                                                                                           >  SRR4302545.481727/1‑94 (MQ=60)
    CACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTC                                                                                           >  SRR4302545.8555236/1‑94 (MQ=60)
     gtgttattgcgtCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCC                                                                                          >  SRR4302545.8659289/13‑94 (MQ=60)
     ACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCC                                                                                          >  SRR4302545.3040017/1‑94 (MQ=60)
     ACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCC                                                                                          >  SRR4302545.7762098/1‑94 (MQ=60)
     ACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCC                                                                                          >  SRR4302545.2322182/1‑94 (MQ=60)
      CTGGATTATTACTGGCGATTGTCATTCGCCTGCCGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTACTCGCCGAACCAGCGCTCCC                                                                                         >  SRR4302545.3513436/1‑94 (MQ=60)
      CTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCC                                                                                         >  SRR4302545.3513772/1‑94 (MQ=60)
      CTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCA                                                                                         >  SRR4302545.9252901/1‑94 (MQ=60)
      CTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCA                                                                                         >  SRR4302545.10856437/1‑94 (MQ=60)
       TGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCAC                                                                                        >  SRR4302545.7225764/1‑94 (MQ=60)
       TGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCAC                                                                                        >  SRR4302545.7225763/1‑94 (MQ=60)
        GGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACC                                                                                       <  SRR4302545.2690978/94‑1 (MQ=60)
        GGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACC                                                                                       >  SRR4302545.8015108/1‑94 (MQ=60)
         GATTATTAGTGGCGATTCTCATCCGCCTGACGCAATAACACGCGGCTTTCGCTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCT                                                                                      <  SRR4302545.3546513/94‑1 (MQ=60)
         GATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAAGCAGCGCTCCACCT                                                                                      >  SRR4302545.4256196/1‑94 (MQ=60)
         GATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCT                                                                                      >  SRR4302545.6168845/1‑94 (MQ=60)
          ATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTT                                                                                     <  SRR4302545.1503607/94‑1 (MQ=60)
          ATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTT                                                                                     <  SRR4302545.6932509/94‑1 (MQ=60)
          ATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTT                                                                                     >  SRR4302545.6379828/1‑94 (MQ=60)
          ATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTT                                                                                     <  SRR4302545.9090166/94‑1 (MQ=60)
            TATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGC                                                                                   >  SRR4302545.2515557/1‑94 (MQ=60)
            TATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGC                                                                                   <  SRR4302545.6082077/94‑1 (MQ=60)
            TATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGC                                                                                   <  SRR4302545.9857870/94‑1 (MQ=60)
             ATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCG                                                                                  <  SRR4302545.5264410/94‑1 (MQ=60)
             ATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCG                                                                                  <  SRR4302545.5696316/94‑1 (MQ=60)
              TTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGG                                                                                 >  SRR4302545.8271738/1‑94 (MQ=60)
              TTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGG                                                                                 <  SRR4302545.990185/94‑1 (MQ=60)
              TTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGG                                                                                 >  SRR4302545.6937492/1‑94 (MQ=60)
               TACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCCCCTTGCGGA                                                                                <  SRR4302545.4302564/94‑1 (MQ=60)
                TCTGGCTATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAA                                                                               <  SRR4302545.10506788/94‑1 (MQ=60)
                ACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAA                                                                               <  SRR4302545.5761988/94‑1 (MQ=60)
                ACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAA                                                                               >  SRR4302545.8055423/1‑94 (MQ=60)
                   GGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACT                                                                            <  SRR4302545.10252326/94‑1 (MQ=60)
                   GGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACT                                                                            <  SRR4302545.6810698/94‑1 (MQ=60)
                     gcATTGTAATTCGCCTGACACAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTACCCCTTGCGGAAACTGT                                                                          <  SRR4302545.3019240/92‑1 (MQ=60)
                      GATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTC                                                                         >  SRR4302545.5657022/1‑94 (MQ=60)
                       ATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCA                                                                        >  SRR4302545.5658228/1‑94 (MQ=60)
                       ATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCA                                                                        >  SRR4302545.8410658/1‑94 (MQ=60)
                        TTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAA                                                                       <  SRR4302545.2989446/94‑1 (MQ=60)
                        TTGTCATTCCCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAA                                                                       <  SRR4302545.2989447/94‑1 (MQ=60)
                         TGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAAT                                                                      >  SRR4302545.1583932/1‑94 (MQ=60)
                         TGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCGTGCGGAAACTGTCAAT                                                                      >  SRR4302545.6748523/1‑94 (MQ=60)
                          GTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATA                                                                     >  SRR4302545.2558962/1‑94 (MQ=60)
                          GTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATA                                                                     <  SRR4302545.5321845/94‑1 (MQ=60)
                           TCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAA                                                                    <  SRR4302545.7375647/94‑1 (MQ=60)
                           TCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAA                                                                    <  SRR4302545.3016434/94‑1 (MQ=60)
                           TCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAA                                                                    <  SRR4302545.1768842/94‑1 (MQ=60)
                           TCATTCCCCTGACGCAAAAACCCGGGGCTTTCACTCTGAAAACCCTGTTCGTAATCCCCGAACCAGCGCCCCACCTTGCGGAAACTGTCAATAA                                                                    <  SRR4302545.2205283/94‑1 (MQ=53)
                            CATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAA                                                                   >  SRR4302545.151728/1‑94 (MQ=60)
                            CATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAA                                                                   <  SRR4302545.7322570/94‑1 (MQ=60)
                            CATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAA                                                                   >  SRR4302545.10663387/1‑94 (MQ=60)
                             gattacgcacagcgttttCACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAAC                                                                  >  SRR4302545.3530228/19‑94 (MQ=60)
                             ATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAAC                                                                  <  SRR4302545.4770276/94‑1 (MQ=60)
                             ATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAAC                                                                  >  SRR4302545.1791205/1‑94 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 <  SRR4302545.326641/94‑1 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 <  SRR4302545.386670/94‑1 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 <  SRR4302545.3003148/94‑1 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 <  SRR4302545.1866820/94‑1 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 <  SRR4302545.7897804/94‑1 (MQ=60)
                                CGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCC                                                               >  SRR4302545.10042618/1‑94 (MQ=60)
                                CGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCC                                                               <  SRR4302545.1261458/94‑1 (MQ=60)
                                CCCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCC                                                               <  SRR4302545.7864128/94‑1 (MQ=60)
                                 GCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCT                                                              <  SRR4302545.5904514/94‑1 (MQ=60)
                                 GCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCT                                                              <  SRR4302545.9619628/94‑1 (MQ=60)
                                 GCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCT                                                              >  SRR4302545.3237007/1‑94 (MQ=60)
                                  CCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTG                                                             <  SRR4302545.4210579/94‑1 (MQ=60)
                                  CCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTG                                                             <  SRR4302545.2232112/94‑1 (MQ=60)
                                  CCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTG                                                             <  SRR4302545.2954811/94‑1 (MQ=60)
                                   CTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGC                                                            <  SRR4302545.1213162/94‑1 (MQ=60)
                                   CTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGC                                                            >  SRR4302545.8426379/1‑94 (MQ=60)
                                   CTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGC                                                            >  SRR4302545.9658722/1‑94 (MQ=60)
                                   CTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGC                                                            <  SRR4302545.9808414/94‑1 (MQ=60)
                                    TGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCT                                                           <  SRR4302545.9912233/94‑1 (MQ=60)
                                     GACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTT                                                          >  SRR4302545.9905438/1‑94 (MQ=60)
                                     GACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTT                                                          >  SRR4302545.9905437/1‑94 (MQ=60)
                                     GACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTT                                                          <  SRR4302545.8487271/94‑1 (MQ=60)
                                     GACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTT                                                          >  SRR4302545.3153543/1‑94 (MQ=60)
                                     GACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTT                                                          <  SRR4302545.866268/94‑1 (MQ=60)
                                     GACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGACTGCTT                                                          >  SRR4302545.10057025/1‑94 (MQ=60)
                                      ACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTA                                                         <  SRR4302545.5120075/94‑1 (MQ=60)
                                       CGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTAT                                                        <  SRR4302545.3193909/94‑1 (MQ=60)
                                        GCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATC                                                       >  SRR4302545.1992596/1‑94 (MQ=60)
                                        GCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATC                                                       >  SRR4302545.3072830/1‑94 (MQ=60)
                                        GCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATC                                                       >  SRR4302545.860235/1‑94 (MQ=60)
                                         caataatgcggggttttcaatcggaataccCTGTGCGTAATCGCCTAACCAGCGCTCCACTTTGGGGAAACTGTCAATAAACGCCTGCTTATCG                                                      <  SRR4302545.1845707/64‑1 (MQ=60)
                                         CAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCG                                                      >  SRR4302545.3726277/1‑94 (MQ=60)
                                         CAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCG                                                      <  SRR4302545.1845708/94‑1 (MQ=60)
                                         CAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCG                                                      >  SRR4302545.311418/1‑94 (MQ=60)
                                           ATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTCATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCC                                                    >  SRR4302545.9597149/1‑94 (MQ=60)
                                           ATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCC                                                    >  SRR4302545.9111740/1‑94 (MQ=60)
                                           ATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCC                                                    >  SRR4302545.7368637/1‑94 (MQ=60)
                                           ATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCC                                                    >  SRR4302545.3036983/1‑94 (MQ=60)
                                           ATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCC                                                    >  SRR4302545.7368636/1‑94 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  >  SRR4302545.9872151/1‑94 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  <  SRR4302545.7999479/94‑1 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  <  SRR4302545.1997829/94‑1 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  <  SRR4302545.3290387/94‑1 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  <  SRR4302545.8624843/94‑1 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  <  SRR4302545.5248175/94‑1 (MQ=60)
                                              ACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTG                                                 <  SRR4302545.7299308/94‑1 (MQ=60)
                                                 CGCGGCTTTCACTCTGAAAACGCTGTGCATAATCGACGAAACCGCGCTCCACCTTGCGGAAACTGTCTATCAACGCCTGCTTATCGCCCTGCAC                                              <  SRR4302545.8312784/94‑1 (MQ=60)
                                                  GCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCC                                             <  SRR4302545.8007128/94‑1 (MQ=60)
                                                    GGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAG                                           >  SRR4302545.195136/1‑94 (MQ=60)
                                                    GGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAG                                           <  SRR4302545.1844098/94‑1 (MQ=60)
                                                    GGCTTTCACTATGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAG                                           >  SRR4302545.1886253/1‑94 (MQ=60)
                                                     GCTTTCCCTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGC                                          <  SRR4302545.8273930/94‑1 (MQ=60)
                                                     GCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGC                                          >  SRR4302545.10850387/1‑94 (MQ=60)
                                                     GCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGC                                          >  SRR4302545.7303360/1‑94 (MQ=60)
                                                      CTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCA                                         >  SRR4302545.903552/1‑94 (MQ=60)
                                                      CTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCA                                         <  SRR4302545.1016039/94‑1 (MQ=60)
                                                        TTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAAC                                       <  SRR4302545.6899650/94‑1 (MQ=60)
                                                         TCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACT                                      <  SRR4302545.1664424/94‑1 (MQ=60)
                                                          CACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTC                                     >  SRR4302545.2217868/1‑94 (MQ=60)
                                                           ACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCCACTCA                                    >  SRR4302545.4221585/1‑94 (MQ=60)
                                                           ACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCA                                    <  SRR4302545.8887562/94‑1 (MQ=60)
                                                            CTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAA                                   <  SRR4302545.2754533/94‑1 (MQ=60)
                                                            CTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAA                                   >  SRR4302545.7347159/1‑94 (MQ=60)
                                                             TCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAT                                  <  SRR4302545.2839918/94‑1 (MQ=60)
                                                             TCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAT                                  <  SRR4302545.1350524/94‑1 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATC                                 >  SRR4302545.3980174/1‑94 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATC                                 >  SRR4302545.2007748/1‑94 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATC                                 <  SRR4302545.1419042/94‑1 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATC                                 <  SRR4302545.10424664/94‑1 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTCCTCCAGCAACTCAATC                                 >  SRR4302545.1753291/1‑94 (MQ=60)
                                                               TGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCG                                >  SRR4302545.4215352/1‑94 (MQ=60)
                                                               TGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCG                                <  SRR4302545.10560058/94‑1 (MQ=60)
                                                               TGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCG                                <  SRR4302545.4809747/94‑1 (MQ=60)
                                                                GAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGC                               <  SRR4302545.3599369/94‑1 (MQ=60)
                                                                GAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGC                               >  SRR4302545.1494323/1‑94 (MQ=60)
                                                                 AAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCC                              >  SRR4302545.3334590/1‑94 (MQ=60)
                                                                  AAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCT                             <  SRR4302545.10367747/94‑1 (MQ=60)
                                                                  AAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCT                             <  SRR4302545.2978386/94‑1 (MQ=60)
                                                                  AAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCT                             <  SRR4302545.11041/94‑1 (MQ=60)
                                                                  AAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCT                             >  SRR4302545.9642935/1‑94 (MQ=60)
                                                                  AAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCT                             <  SRR4302545.6707260/94‑1 (MQ=60)
                                                                  AAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCT                             >  SRR4302545.3252263/1‑94 (MQ=60)
                                                                  AAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAGCGCCT                             <  SRR4302545.10367745/94‑1 (MQ=60)
                                                                   AACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTC                            >  SRR4302545.9459005/1‑94 (MQ=60)
                                                                   AACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTC                            <  SRR4302545.3180170/94‑1 (MQ=60)
                                                                   AACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTC                            <  SRR4302545.3180171/94‑1 (MQ=60)
                                                                   AACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTC                            <  SRR4302545.8618059/94‑1 (MQ=60)
                                                                    ACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCG                           <  SRR4302545.10624759/94‑1 (MQ=60)
                                                                    ACGCTGAGCGTAGTCGCCGACCCAGCGCTCCACCTTGCGGAAACTGCCATTAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCG                           <  SRR4302545.9521106/94‑1 (MQ=60)
                                                                      GCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCC                         <  SRR4302545.10228679/94‑1 (MQ=60)
                                                                      GCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCC                         <  SRR4302545.6982836/94‑1 (MQ=60)
                                                                      GCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCC                         >  SRR4302545.6040935/1‑94 (MQ=60)
                                                                         GTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAA                      >  SRR4302545.10010549/1‑94 (MQ=60)
                                                                         GTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAA                      >  SRR4302545.5633908/1‑94 (MQ=60)
                                                                         GTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAA                      <  SRR4302545.697443/94‑1 (MQ=60)
                                                                         GTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAA                      >  SRR4302545.4054418/1‑94 (MQ=60)
                                                                           GCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAAC                    <  SRR4302545.6836416/94‑1 (MQ=60)
                                                                            CGTAATCGCCGAACCAGCGCTCCTCCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACG                   <  SRR4302545.6304728/94‑1 (MQ=60)
                                                                            CGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACG                   <  SRR4302545.6023141/94‑1 (MQ=60)
                                                                            CGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACG                   >  SRR4302545.2983105/1‑94 (MQ=60)
                                                                             GTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGC                  >  SRR4302545.9477018/1‑94 (MQ=60)
                                                                             GTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGC                  >  SRR4302545.5365649/1‑94 (MQ=60)
                                                                               AATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTT                >  SRR4302545.3294098/1‑94 (MQ=60)
                                                                               AATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTT                >  SRR4302545.4609958/1‑94 (MQ=60)
                                                                               AATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTT                <  SRR4302545.3040450/94‑1 (MQ=60)
                                                                                ATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTA               <  SRR4302545.5881674/94‑1 (MQ=60)
                                                                                ATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTA               <  SRR4302545.10154233/94‑1 (MQ=60)
                                                                                ATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTA               >  SRR4302545.6662764/1‑94 (MQ=60)
                                                                                 TCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTAT              <  SRR4302545.9810605/94‑1 (MQ=60)
                                                                                 TCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTAT              <  SRR4302545.5273838/94‑1 (MQ=60)
                                                                                   GCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAG            >  SRR4302545.10396348/1‑94 (MQ=60)
                                                                                   GCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAG            <  SRR4302545.7271009/94‑1 (MQ=60)
                                                                                    CCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGT           <  SRR4302545.353279/94‑1 (MQ=60)
                                                                                    CCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCCGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGT           >  SRR4302545.9578939/1‑94 (MQ=60)
                                                                                     CGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTA          <  SRR4302545.7917190/94‑1 (MQ=60)
                                                                                      GAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAA         >  SRR4302545.7432061/1‑94 (MQ=60)
                                                                                      GAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAA         <  SRR4302545.6752083/94‑1 (MQ=60)
                                                                                      GAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAA         <  SRR4302545.9897776/94‑1 (MQ=60)
                                                                                      GAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAA         <  SRR4302545.10234412/94‑1 (MQ=60)
                                                                                        ACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACG       >  SRR4302545.8855178/1‑94 (MQ=60)
                                                                                         CCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGT      <  SRR4302545.1118762/94‑1 (MQ=60)
                                                                                         CCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCGCGCCGAAACGCTTATAGTAACGT      <  SRR4302545.4969563/94‑1 (MQ=60)
                                                                                          CAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTT     >  SRR4302545.6206830/1‑94 (MQ=60)
                                                                                           AGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTT    <  SRR4302545.7522548/94‑1 (MQ=60)
                                                                                            GCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTG   >  SRR4302545.8115544/1‑94 (MQ=60)
                                                                                            GCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTG   <  SRR4302545.9083086/94‑1 (MQ=60)
                                                                                             CGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  <  SRR4302545.5643433/94‑1 (MQ=60)
                                                                                             CGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  >  SRR4302545.8362548/1‑94 (MQ=60)
                                                                                             CGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  >  SRR4302545.7799656/1‑94 (MQ=60)
                                                                                             CGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  <  SRR4302545.7519326/94‑1 (MQ=60)
                                                                                             CGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  <  SRR4302545.7356092/94‑1 (MQ=60)
                                                                                             CGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTACAGTAACGTTTGA  <  SRR4302545.6337172/94‑1 (MQ=60)
                                                                                             AGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  <  SRR4302545.7203704/94‑1 (MQ=60)
                                                                                             AGCTCCACCTTGCGGAAACTGTCAATAAACGCCGGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  <  SRR4302545.7203703/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  >  NC_000913/2736954‑2737140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑