Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,737,047 T→C H348R (CAC→CGC)  tyrA ← chorismate mutase‑T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,737,0470TC99.6% 769.8 / ‑5.6 224H348R (CAC→CGC) tyrAchorismate mutase‑T
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (87/136);  minor base A (1/0);  total (88/136)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 233 total aligned reads displayed.

GCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGT  >  NC_000913/2736957‑2737136
                                                                                          |                                                                                         
gCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAgcgc                                                                                         >  2:3508752/1‑93 (MQ=255)
gCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAgcgc                                                                                         <  2:2280694/93‑1 (MQ=255)
gCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAgcgc                                                                                         <  1:1865827/93‑1 (MQ=255)
   cTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTcc                                                                                      <  1:191946/93‑1 (MQ=255)
   cTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTcc                                                                                      <  2:2590237/93‑1 (MQ=255)
   cTGGATTATTACTGGCGATTGTCATTCGCCCGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTcc                                                                                      >  1:3938993/1‑93 (MQ=255)
    tGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTccc                                                                                     >  1:4395742/1‑92 (MQ=255)
      gATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCAcc                                                                                   >  1:5652961/1‑93 (MQ=255)
       attattACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCt                                                                                  >  2:5472640/1‑93 (MQ=255)
       attattACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCt                                                                                  <  1:1163238/93‑1 (MQ=255)
        ttattaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCtt                                                                                 <  2:1775741/93‑1 (MQ=255)
        ttattaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCtt                                                                                 >  2:3992011/1‑93 (MQ=255)
         tattaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTg                                                                                >  1:5690755/1‑93 (MQ=255)
         tattaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTg                                                                                >  2:3713169/1‑93 (MQ=255)
         tattaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTg                                                                                >  1:4654144/1‑93 (MQ=255)
           ttaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCg                                                                              <  2:2168043/93‑1 (MQ=255)
           ttaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCg                                                                              >  1:240318/1‑93 (MQ=255)
           ttaCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCg                                                                              <  1:5396480/93‑1 (MQ=255)
            taCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCgg                                                                             >  1:6186565/1‑93 (MQ=255)
             aCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGa                                                                            >  1:2631522/1‑93 (MQ=255)
             aCTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGa                                                                            <  2:6445046/93‑1 (MQ=255)
              cTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGaa                                                                           >  1:2718124/1‑93 (MQ=255)
              cTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGaa                                                                           >  1:1559182/1‑93 (MQ=255)
              cTGGCGATTGTCATCCGCCTGACGCAATAACACGCGGCTTGCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGaa                                                                           <  1:3080710/93‑1 (MQ=255)
                 gCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACt                                                                        <  1:3218182/93‑1 (MQ=255)
                 gCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACt                                                                        <  2:3601830/93‑1 (MQ=255)
                  cGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCTCCTTGCGGAAACTg                                                                       <  2:2902996/93‑1 (MQ=255)
                  cGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTg                                                                       >  1:1612236/1‑93 (MQ=255)
                   gATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGt                                                                      <  1:4976386/93‑1 (MQ=255)
                     ttGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCa                                                                    <  1:2767631/93‑1 (MQ=255)
                      tGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCaa                                                                   >  2:4617346/1‑93 (MQ=255)
                        tCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATa                                                                 >  1:877463/1‑93 (MQ=255)
                        tCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATa                                                                 <  2:4985382/93‑1 (MQ=255)
                        tCAGTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATa                                                                 <  1:5360043/93‑1 (MQ=255)
                           ttCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAAc                                                              <  1:1009558/93‑1 (MQ=255)
                            tCGCCTGACGCAATAACACGCTGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACg                                                             <  2:5733411/93‑1 (MQ=255)
                            tCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACg                                                             <  2:5928882/93‑1 (MQ=255)
                                 tGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGc                                                        <  2:2250674/93‑1 (MQ=255)
                                  gACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCt                                                       <  1:2096884/93‑1 (MQ=255)
                                  gACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCt                                                       <  2:1089357/93‑1 (MQ=255)
                                   aCGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCtt                                                      <  2:4541715/93‑1 (MQ=255)
                                    cGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTa                                                     <  2:3881001/93‑1 (MQ=255)
                                     gCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTAt                                                    >  2:1749939/1‑93 (MQ=255)
                                      cAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATc                                                   <  2:2719299/93‑1 (MQ=255)
                                      cAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATc                                                   <  2:3489036/93‑1 (MQ=255)
                                      cAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATc                                                   <  2:4262398/93‑1 (MQ=255)
                                        aTAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGc                                                 <  2:1507066/93‑1 (MQ=255)
                                          aaCACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGccc                                               <  2:5641783/93‑1 (MQ=255)
                                          aaCACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGccc                                               <  2:2442097/93‑1 (MQ=255)
                                          aaCACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGccc                                               <  1:4218089/93‑1 (MQ=255)
                                           acacGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAATGCCTGCTTATCGCCCt                                              <  2:2704394/93‑1 (MQ=255)
                                           acacGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTGATCGCCCt                                              <  2:6283368/93‑1 (MQ=255)
                                            cacGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTg                                             <  1:3151116/93‑1 (MQ=255)
                                            cacGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTg                                             >  1:1278575/1‑93 (MQ=255)
                                             acGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGc                                            >  2:3522755/1‑93 (MQ=255)
                                               gcgGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTc                                          >  2:4378464/1‑93 (MQ=255)
                                                cgGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTcc                                         <  1:6273702/93‑1 (MQ=255)
                                                cgGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTcc                                         <  1:895553/93‑1 (MQ=255)
                                                  gCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAg                                       >  1:683142/1‑93 (MQ=255)
                                                   cTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGc                                      >  2:3129327/1‑93 (MQ=255)
                                                   cTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGAGCTCCACCTTGCGGAAACTGTCAATACACGCCTGCTTATCGCCCTGCTCCAGc                                      >  1:1767550/1‑93 (MQ=255)
                                                      tCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAAc                                   >  2:4858348/1‑93 (MQ=255)
                                                      tCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAAc                                   >  1:583593/1‑93 (MQ=255)
                                                       cACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACt                                  >  2:530031/1‑93 (MQ=255)
                                                        aCTCTGAAAACGCTGTGCGTATTCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTc                                 >  2:5995990/1‑93 (MQ=255)
                                                        aCTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTc                                 >  2:840833/1‑93 (MQ=255)
                                                        aCTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTc                                 >  1:3530813/1‑93 (MQ=255)
                                                        aCTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTc                                 >  2:183254/1‑93 (MQ=255)
                                                          tctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCaa                               <  1:338151/93‑1 (MQ=255)
                                                          tctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCaa                               >  2:3013279/1‑93 (MQ=255)
                                                          tctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCaa                               <  1:888692/93‑1 (MQ=255)
                                                           ctGAAAACGCTGTGCGTAATCGCCTAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAt                              <  2:1175264/93‑1 (MQ=255)
                                                           ctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAt                              <  2:2214562/93‑1 (MQ=255)
                                                           ctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAt                              <  1:3953227/93‑1 (MQ=255)
                                                           ctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAt                              <  1:198972/93‑1 (MQ=255)
                                                           ctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAt                              <  2:1554885/93‑1 (MQ=255)
                                                           ctGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAt                              <  1:1385383/93‑1 (MQ=255)
                                                             gAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcg                            >  2:3211392/1‑93 (MQ=255)
                                                              aaaaCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgc                           >  1:6061404/1‑93 (MQ=255)
                                                               aaaCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcc                          >  1:5071708/1‑93 (MQ=255)
                                                                  cGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcctcg                       >  2:5760542/1‑93 (MQ=255)
                                                                    cTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAtcgcctcgcc                     >  1:197288/1‑93 (MQ=255)
                                                                     tgtgCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCg                    >  1:284416/1‑93 (MQ=255)
                                                                      gtgCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGa                   <  2:2044406/93‑1 (MQ=255)
                                                                        gCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGaaa                 <  2:1330494/93‑1 (MQ=255)
                                                                        gCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGaaa                 <  2:5426075/93‑1 (MQ=255)
                                                                         cGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAAc                <  1:5559462/93‑1 (MQ=255)
                                                                         cGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAAc                <  2:1683744/93‑1 (MQ=255)
                                                                           tAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGc              <  1:4495600/93‑1 (MQ=255)
                                                                            aaTCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCt             >  1:3741095/1‑93 (MQ=255)
                                                                             aTCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCtt            >  2:4785332/1‑93 (MQ=255)
                                                                              tCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTta           <  1:5418674/93‑1 (MQ=255)
                                                                              tCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTta           <  2:2001410/93‑1 (MQ=255)
                                                                                 ccGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAg        <  1:1030381/93‑1 (MQ=255)
                                                                                 ccGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAg        <  2:4652133/93‑1 (MQ=255)
                                                                                  cGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGt       <  2:3344641/93‑1 (MQ=255)
                                                                                   gAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTa      <  1:5224987/93‑1 (MQ=255)
                                                                                    aaCCAGCGCTCCACCTTGCGGAACCTGTCAATAACCGCCTGCTTATCGCCCTGCTCCAGCACCTCAATCGCCTCGCCGAAACGCTTATAGTaa     <  1:6306817/93‑1 (MQ=255)
                                                                                    aaCCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTaa     >  1:2986189/1‑93 (MQ=255)
                                                                                       cAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGt  <  2:721459/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGT  >  NC_000913/2736957‑2737136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  >  NC_000913/2736954‑2737140
                                                                                             |                                                                                             
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGC                                                                                               <  SRR4302537.3424237/94‑1 (MQ=60)
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGC                                                                                               >  SRR4302537.799444/1‑94 (MQ=60)
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGC                                                                                               <  SRR4302537.2268477/94‑1 (MQ=60)
  GGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGC                                                                                             <  SRR4302537.4667678/94‑1 (MQ=60)
   GCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCT                                                                                            <  SRR4302537.1981164/94‑1 (MQ=60)
   GCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCT                                                                                            <  SRR4302537.1878819/94‑1 (MQ=60)
     ACTGGATTATTACTGGCGATTGTCATTCGCCCGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCC                                                                                          >  SRR4302537.3966530/1‑94 (MQ=60)
      CTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCC                                                                                         >  SRR4302537.4426066/1‑94 (MQ=60)
      CTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCA                                                                                         <  SRR4302537.193307/94‑1 (MQ=60)
        GGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACC                                                                                       >  SRR4302537.5691646/1‑94 (MQ=60)
          ATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTT                                                                                     <  SRR4302537.1171325/94‑1 (MQ=60)
           TTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTG                                                                                    >  SRR4302537.4686329/1‑94 (MQ=60)
           TTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTG                                                                                    >  SRR4302537.5729678/1‑94 (MQ=60)
            TATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGC                                                                                   <  SRR4302537.3353628/94‑1 (MQ=60)
             ATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCG                                                                                  >  SRR4302537.242020/1‑94 (MQ=60)
             ATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCG                                                                                  <  SRR4302537.5500286/94‑1 (MQ=60)
             ATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCG                                                                                  >  SRR4302537.3896006/1‑94 (MQ=60)
              TTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGG                                                                                 <  SRR4302537.5433328/94‑1 (MQ=60)
              TTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGG                                                                                 >  SRR4302537.5406213/1‑94 (MQ=60)
              TTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGG                                                                                 <  SRR4302537.5605731/94‑1 (MQ=60)
              TTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGG                                                                                 >  SRR4302537.129692/1‑94 (MQ=60)
              TTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGG                                                                                 >  SRR4302537.6229180/1‑94 (MQ=60)
               TACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGA                                                                                >  SRR4302537.2649947/1‑94 (MQ=60)
               TACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGA                                                                                <  SRR4302537.5304231/94‑1 (MQ=60)
                ACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAA                                                                               >  SRR4302537.1570065/1‑94 (MQ=60)
                ACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAA                                                                               >  SRR4302537.2737105/1‑94 (MQ=60)
                 CTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAA                                                                              >  SRR4302537.2002049/1‑94 (MQ=60)
                 CTGGCGATTGTCATCCGCCTGACGCAATAACACGCGGCTTGCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAA                                                                              <  SRR4302537.3102164/94‑1 (MQ=60)
                  TGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAAC                                                                             >  SRR4302537.431445/1‑94 (MQ=60)
                  TGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAAC                                                                             <  SRR4302537.1317259/94‑1 (MQ=60)
                    GCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTG                                                                           >  SRR4302537.1623503/1‑94 (MQ=60)
                    GCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTG                                                                           <  SRR4302537.3240826/94‑1 (MQ=60)
                     CGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGGAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGT                                                                          >  SRR4302537.888966/1‑94 (MQ=60)
                     CGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGT                                                                          >  SRR4302537.1628136/1‑94 (MQ=60)
                     CGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGT                                                                          <  SRR4302537.2783605/94‑1 (MQ=60)
                      GATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTC                                                                         <  SRR4302537.5010686/94‑1 (MQ=60)
                        TTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAA                                                                       <  SRR4302537.2786975/94‑1 (MQ=60)
                          TTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATA                                                                     <  SRR4302537.1028068/94‑1 (MQ=60)
                          GTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATA                                                                     >  SRR4302537.883499/1‑94 (MQ=60)
                           TCAGTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAA                                                                    <  SRR4302537.5396746/94‑1 (MQ=60)
                             ATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAAC                                                                  <  SRR4302537.2622513/94‑1 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 <  SRR4302537.4985028/94‑1 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 >  SRR4302537.895060/1‑94 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 <  SRR4302537.1016520/94‑1 (MQ=60)
                              TTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACG                                                                 <  SRR4302537.2116462/94‑1 (MQ=60)
                                 GCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCT                                                              >  SRR4302537.333499/1‑94 (MQ=60)
                                 GCCTGACGCAATAACACGCGCCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCT                                                              <  SRR4302537.3568748/94‑1 (MQ=60)
                                   CTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGC                                                            <  SRR4302537.2844976/94‑1 (MQ=60)
                                    TGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCT                                                           >  SRR4302537.3907584/1‑94 (MQ=60)
                                    TGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCT                                                           <  SRR4302537.2743513/94‑1 (MQ=60)
                                     GACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCACTAAACGCCTGCTT                                                          <  SRR4302537.3511733/94‑1 (MQ=60)
                                     GACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTT                                                          <  SRR4302537.2111670/94‑1 (MQ=60)
                                       CGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTAT                                                        <  SRR4302537.5743663/94‑1 (MQ=60)
                                        GCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATC                                                       <  SRR4302537.3168040/94‑1 (MQ=60)
                                         CAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCG                                                      <  SRR4302537.397010/94‑1 (MQ=60)
                                          AATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGC                                                     >  SRR4302537.1000843/1‑94 (MQ=60)
                                          AATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGC                                                     <  SRR4302537.1943809/94‑1 (MQ=60)
                                           ATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCC                                                    <  SRR4302537.1748094/94‑1 (MQ=60)
                                           ATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCC                                                    <  SRR4302537.5113152/94‑1 (MQ=60)
                                            TAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCC                                                   <  SRR4302537.4640504/94‑1 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  >  SRR4302537.6305962/1‑94 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  <  SRR4302537.5439570/94‑1 (MQ=60)
                                             AACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCT                                                  <  SRR4302537.4247489/94‑1 (MQ=60)
                                              ACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTG                                                 >  SRR4302537.1287459/1‑94 (MQ=60)
                                              ACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTG                                                 <  SRR4302537.1202829/94‑1 (MQ=60)
                                               CACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGC                                                >  SRR4302537.4172414/1‑94 (MQ=60)
                                               CACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGC                                                <  SRR4302537.3173212/94‑1 (MQ=60)
                                                   CGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCA                                            <  SRR4302537.1485447/94‑1 (MQ=60)
                                                   CGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCA                                            <  SRR4302537.6316750/94‑1 (MQ=60)
                                                   CGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCA                                            <  SRR4302537.5333987/94‑1 (MQ=60)
                                                   CGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCA                                            <  SRR4302537.901736/94‑1 (MQ=60)
                                                    GGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAG                                           >  SRR4302537.687823/1‑94 (MQ=60)
                                                     GCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGC                                          >  SRR4302537.1874801/1‑94 (MQ=60)
                                                     GCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGC                                          <  SRR4302537.4254009/94‑1 (MQ=60)
                                                     GCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGAGCTCCACCTTGCGGAAACTGTCAATACACGCCTGCTTATCGCCCTGCTCCAGC                                          >  SRR4302537.1779857/1‑94 (MQ=60)
                                                        TTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAAC                                       >  SRR4302537.587581/1‑94 (MQ=60)
                                                        TTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAAC                                       >  SRR4302537.1578887/1‑94 (MQ=60)
                                                        TTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAAC                                       >  SRR4302537.6302357/1‑94 (MQ=60)
                                                          CACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTC                                     >  SRR4302537.3555773/1‑94 (MQ=60)
                                                            CTCTGAAAACGCTGTGCGTAATCCCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAA                                   <  SRR4302537.5102922/94‑1 (MQ=60)
                                                             TCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAT                                  >  SRR4302537.5717629/1‑94 (MQ=60)
                                                             TCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAT                                  <  SRR4302537.340496/94‑1 (MQ=60)
                                                             TCTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAAT                                  <  SRR4302537.894806/94‑1 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATC                                 <  SRR4302537.1395047/94‑1 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATC                                 <  SRR4302537.2194666/94‑1 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATC                                 <  SRR4302537.3980880/94‑1 (MQ=60)
                                                              CTGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATC                                 <  SRR4302537.200390/94‑1 (MQ=60)
                                                               TGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCG                                <  SRR4302537.1894958/94‑1 (MQ=60)
                                                               TGAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCG                                <  SRR4302537.6492328/94‑1 (MQ=60)
                                                                GAAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGC                               >  SRR4302537.6103049/1‑94 (MQ=60)
                                                                 AAAACGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCC                              >  SRR4302537.5106629/1‑94 (MQ=60)
                                                                   ACCGCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTC                            <  SRR4302537.5575048/94‑1 (MQ=60)
                                                                      GCTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCC                         >  SRR4302537.198694/1‑94 (MQ=60)
                                                                       CTGTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCG                        >  SRR4302537.286398/1‑94 (MQ=60)
                                                                         GTGCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAA                      <  SRR4302537.4854011/94‑1 (MQ=60)
                                                                           GCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAAC                    <  SRR4302537.3190644/94‑1 (MQ=60)
                                                                           GCGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAAC                    <  SRR4302537.5941855/94‑1 (MQ=60)
                                                                            CGTAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACG                   <  SRR4302537.5597451/94‑1 (MQ=60)
                                                                              TAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCT                 <  SRR4302537.728618/94‑1 (MQ=60)
                                                                              TAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCT                 <  SRR4302537.4526699/94‑1 (MQ=60)
                                                                              TAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCT                 >  SRR4302537.3018082/1‑94 (MQ=60)
                                                                              TAATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCT                 >  SRR4302537.3767369/1‑94 (MQ=60)
                                                                               AATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTT                >  SRR4302537.4438523/1‑94 (MQ=60)
                                                                               AATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTT                <  SRR4302537.1056396/94‑1 (MQ=60)
                                                                                ATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTA               >  SRR4302537.2060675/1‑94 (MQ=60)
                                                                                ATCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTA               <  SRR4302537.6187614/94‑1 (MQ=60)
                                                                                 TCGCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTAT              <  SRR4302537.5455607/94‑1 (MQ=60)
                                                                                   GCCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAG            <  SRR4302537.2730497/94‑1 (MQ=60)
                                                                                    CCGAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGT           <  SRR4302537.1037477/94‑1 (MQ=60)
                                                                                      GAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAA         <  SRR4302537.5260918/94‑1 (MQ=60)
                                                                                      GAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAA         >  SRR4302537.3007054/1‑94 (MQ=60)
                                                                                      GAACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAA         <  SRR4302537.2880666/94‑1 (MQ=60)
                                                                                       AACCAGCGCTCCACCTTGCGGAACCTGTCAATAACCGCCTGCTTATCGCCCTGCTCCAGCACCTCAATCGCCTCGCCGAAACGCTTATAGTAAC        <  SRR4302537.6350004/94‑1 (MQ=60)
                                                                                       AACCAGCGCTCCACCTTGCGGAACCTGTCAATAACCGCCTGCTTATCGCCCTGCTCCAGCACCTCAATCGCCTCGCCGAAACGCTTATAGTAAC        <  SRR4302537.6350003/94‑1 (MQ=60)
                                                                                        ACCAGCGCTCCACCTTGCGGAAACTGTCAATAAACTCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACG       <  SRR4302537.307751/94‑1 (MQ=60)
                                                                                         CCAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGT      <  SRR4302537.827321/94‑1 (MQ=60)
                                                                                          CAGCGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTT     <  SRR4302537.580188/94‑1 (MQ=60)
                                                                                             CGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  <  SRR4302537.5820523/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGA  >  NC_000913/2736954‑2737140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑