Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,820,999 T→G Y265S (TAC→TCC)  recA ← RecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,820,9990TG99.3% 1407.5 / ‑3.1 405Y265S (TAC→TCC) recARecA
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base G (212/190);  total (215/190)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 417 total aligned reads displayed.

TTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGC  >  NC_000913/2820908‑2821089
                                                                                           |                                                                                          
  tGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGagag                                                                                        <  2:1243683/94‑1 (MQ=255)
     aGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGAt                                                                                     <  2:6304887/94‑1 (MQ=255)
      gCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATc                                                                                    >  2:3017873/1‑94 (MQ=255)
      gCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATc                                                                                    <  1:903498/94‑1 (MQ=255)
       cTGTCCCGCGCGCCTGCTTTCTCGCTCAGCTTCTCTTTTCCGCCCGGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGCGGATCt                                                                                   <  2:9361184/94‑1 (MQ=255)
       cTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCt                                                                                   >  2:4831584/1‑94 (MQ=255)
        tGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTg                                                                                  >  1:10523835/1‑94 (MQ=255)
        tGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTg                                                                                  <  2:908501/94‑1 (MQ=255)
        tGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTg                                                                                  >  1:236896/1‑94 (MQ=255)
          tACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGa                                                                                <  2:7501175/94‑1 (MQ=255)
           aCCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGat                                                                               >  2:7786889/1‑93 (MQ=255)
           aCCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTCACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGaa                                                                               >  1:3092376/1‑94 (MQ=255)
            ccACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAt                                                                              >  2:4204405/1‑94 (MQ=255)
            ccACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAt                                                                              <  1:1317691/94‑1 (MQ=255)
             cACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAtt                                                                             >  2:2453360/1‑94 (MQ=255)
             cACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAtt                                                                             >  2:2847106/1‑94 (MQ=255)
             cACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAtt                                                                             >  1:4876468/1‑94 (MQ=255)
                gcgcCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAg                                                                          <  2:1548739/94‑1 (MQ=255)
                gcgcCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAg                                                                          <  2:6620612/94‑1 (MQ=255)
                 cgcCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGc                                                                         >  2:5888357/1‑94 (MQ=255)
                 cgcCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGc                                                                         >  1:6445174/1‑94 (MQ=255)
                  gcCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGcc                                                                        >  2:1057650/1‑94 (MQ=255)
                  gcCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGcc                                                                        >  1:5732407/1‑94 (MQ=255)
                    cTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTg                                                                      >  2:3168271/1‑94 (MQ=255)
                     tGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGt                                                                     <  1:2534682/94‑1 (MQ=255)
                      gCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGtt                                                                    >  2:8043000/1‑94 (MQ=255)
                        tttCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTa                                                                  >  1:598171/1‑94 (MQ=255)
                        tttCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTa                                                                  <  1:8176028/94‑1 (MQ=255)
                           ctcGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAAc                                                               <  2:7221264/94‑1 (MQ=255)
                           ctcGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAAc                                                               <  1:2887494/94‑1 (MQ=255)
                           ctcGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAAc                                                               <  2:5597081/94‑1 (MQ=255)
                             cGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACgg                                                             >  1:6575936/1‑94 (MQ=255)
                              gATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGgc                                                            <  1:3767458/94‑1 (MQ=255)
                              gATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATGCAGCCTGTTTAAACGgc                                                            >  2:6523014/1‑94 (MQ=255)
                              gATCAGCTTCTCTTTTACGCCCAGGGCAACCAGGTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGGTCTGGAATTCAGACTGTTTAGACGgc                                                            >  1:3640941/1‑94 (MQ=255)
                               aTCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGgcg                                                           >  2:6042546/1‑94 (MQ=255)
                               aTCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGgcg                                                           <  1:9151376/94‑1 (MQ=255)
                                tCAGCTTCTGTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATCCCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGgcgc                                                          <  2:8178176/94‑1 (MQ=255)
                                tCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGgcgc                                                          >  1:717041/1‑94 (MQ=255)
                                tCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGgcgc                                                          <  1:4259701/94‑1 (MQ=255)
                                 cAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCa                                                         >  2:9249917/1‑94 (MQ=255)
                                 cAGCTTCCCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTACACGGCGCa                                                         >  2:2624690/1‑94 (MQ=255)
                                  aGCTCCTCTTTCACGCCCAGGCCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAg                                                        <  1:9782267/94‑1 (MQ=255)
                                   gCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGGCTGTTTAAACGGCGCAGc                                                       >  1:2266490/1‑94 (MQ=255)
                                   gCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGc                                                       <  1:8879463/94‑1 (MQ=255)
                                   gCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGc                                                       <  2:1072824/94‑1 (MQ=255)
                                    cTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCg                                                      <  1:590223/94‑1 (MQ=255)
                                     ttCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGa                                                     >  1:8105448/1‑94 (MQ=255)
                                      tctcTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGAt                                                    <  1:5234483/94‑1 (MQ=255)
                                           tttACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGt                                               <  2:2174618/94‑1 (MQ=255)
                                            ttACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGtt                                              <  1:1929795/94‑1 (MQ=255)
                                              aCGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGttct                                            >  2:3726334/1‑94 (MQ=255)
                                                gCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGttcttc                                          >  2:6423942/1‑94 (MQ=255)
                                                 cccAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTca                                         >  2:1793397/1‑94 (MQ=255)
                                                 cccAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTca                                         >  2:8847857/1‑94 (MQ=255)
                                                 cccAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTca                                         <  1:7963481/94‑1 (MQ=255)
                                                   cAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcacc                                       >  2:3375106/1‑94 (MQ=255)
                                                   cAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcacc                                       >  2:7741415/1‑94 (MQ=255)
                                                    aGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTcacca                                      >  1:4621258/1‑94 (MQ=255)
                                                      gTCAACCGGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACt                                    <  1:3236277/94‑1 (MQ=255)
                                                      gTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACt                                    >  1:7571490/1‑94 (MQ=255)
                                                        taaCCGGGTCGTCGTAGAAGGTGATGCCTTCCGCGGAGAGGATCTGGAATTCCGGCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACttt                                  <  2:3514604/93‑1 (MQ=255)
                                                        cAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACttt                                  >  1:8576691/1‑94 (MQ=255)
                                                         aaCCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTc                                 <  2:10555439/94‑1 (MQ=255)
                                                         aaCCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTc                                 >  2:4422322/1‑94 (MQ=255)
                                                          aCCAGTTCGCCGTAGAAGTTGATGCCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCa                                <  2:9724254/94‑1 (MQ=255)
                                                          aCCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGGTTTTGTTCTTCACCACTTTCa                                >  1:1592874/1‑94 (MQ=255)
                                                          aCCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCGGCGATTTTGTTCTTCACCACTTTCa                                >  2:4958617/1‑94 (MQ=255)
                                                          aCCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCa                                >  1:2757131/1‑94 (MQ=255)
                                                           cgaGTCCGCCTTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTGCTTCACCACTTTccc                               <  1:4127646/92‑3 (MQ=255)
                                                           ccAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAc                               >  1:10025816/1‑94 (MQ=255)
                                                            cAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAcg                              <  1:9543180/94‑1 (MQ=255)
                                                            cAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAcg                              >  2:7057857/1‑94 (MQ=255)
                                                             aGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAcgc                             <  1:1739558/94‑1 (MQ=255)
                                                             aGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAcgc                             >  2:8402998/1‑94 (MQ=255)
                                                             aGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAcgc                             >  1:4698172/1‑94 (MQ=255)
                                                             aGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAcgc                             >  1:5659237/1‑94 (MQ=255)
                                                              gTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAcgcg                            <  1:9367739/94‑1 (MQ=255)
                                                               ttCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCgg                           <  1:9241425/94‑1 (MQ=255)
                                                               gtCGGCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCgg                           <  1:2024574/93‑1 (MQ=255)
                                                                tCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCggg                          <  1:6346237/94‑1 (MQ=255)
                                                                 cGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGt                         >  2:6188430/1‑94 (MQ=255)
                                                                  gCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGtt                        <  1:694055/94‑1 (MQ=255)
                                                                  gCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGtt                        >  2:4379086/1‑94 (MQ=255)
                                                                    cGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTc                      >  2:8599982/1‑94 (MQ=255)
                                                                      tAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGc                    >  1:4956027/1‑94 (MQ=255)
                                                                        gAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTa                  <  1:6177799/94‑1 (MQ=255)
                                                                          aGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTAcc                >  1:8242459/1‑94 (MQ=255)
                                                                             tGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACccac             >  1:6842067/1‑94 (MQ=255)
                                                                             tGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACccac             >  1:1021359/1‑94 (MQ=255)
                                                                                tACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCcaccac          >  2:9576381/1‑94 (MQ=255)
                                                                                 aCCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACg         >  2:4664531/1‑94 (MQ=255)
                                                                                  ccTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGt        >  1:7360895/1‑94 (MQ=255)
                                                                                      cGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTc    <  1:8715094/94‑1 (MQ=255)
                                                                                       gCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCg   <  2:1012576/94‑1 (MQ=255)
                                                                                       gCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCg   <  1:3865123/94‑1 (MQ=255)
                                                                                       gCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCg   >  2:10478532/1‑94 (MQ=255)
                                                                                        ccGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGc  >  2:3941444/1‑94 (MQ=255)
                                                                                        ccGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGc  >  1:9875733/1‑94 (MQ=255)
                                                                                        ccGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGc  <  2:4485304/94‑1 (MQ=255)
                                                                                           |                                                                                          
TTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGC  >  NC_000913/2820908‑2821089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 225 total aligned reads displayed.
CTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGCCCT  >  NC_000913/2820907‑2821092
                                                                                            |                                                                                             
CTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGA                                                                                              >  SRR4302545.6852919/1‑94 (MQ=60)
CTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGA                                                                                              <  SRR4302545.1026727/94‑1 (MQ=60)
   TGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAG                                                                                           >  SRR4302545.4526092/1‑94 (MQ=60)
   TGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAG                                                                                           <  SRR4302545.3758597/94‑1 (MQ=60)
     TAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGA                                                                                         >  SRR4302545.5479585/1‑94 (MQ=60)
     TAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGA                                                                                         <  SRR4302545.9790530/94‑1 (MQ=60)
       GCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATC                                                                                       <  SRR4302545.923750/94‑1 (MQ=60)
       GCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATC                                                                                       >  SRR4302545.8332987/1‑94 (MQ=60)
       GCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATC                                                                                       <  SRR4302545.7281643/94‑1 (MQ=60)
       GCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGCTGATACCTTCGCCGGAGCGGATC                                                                                       >  SRR4302545.8332988/1‑94 (MQ=60)
        CTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCT                                                                                      <  SRR4302545.8255712/94‑1 (MQ=60)
        CTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCT                                                                                      <  SRR4302545.6128962/94‑1 (MQ=60)
         TGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTG                                                                                     <  SRR4302545.4212885/94‑1 (MQ=60)
         TGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTG                                                                                     >  SRR4302545.241783/1‑94 (MQ=60)
         TGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTG                                                                                     >  SRR4302545.674911/1‑94 (MQ=60)
         TGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTG                                                                                     >  SRR4302545.10833879/1‑94 (MQ=60)
          GTCCCACGCCCCTGCTTTCTGGATCAGCTTGTCTTTTTCGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATGCCTTCGCCGGAGAGGATCTGG                                                                                    <  SRR4302545.9102363/94‑1 (MQ=60)
          GTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGG                                                                                    <  SRR4302545.2560591/94‑1 (MQ=60)
          GTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGG                                                                                    >  SRR4302545.4789571/1‑94 (MQ=60)
           TACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGA                                                                                   <  SRR4302545.10146039/94‑1 (MQ=60)
            ACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTCACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAA                                                                                  >  SRR4302545.3168527/1‑94 (MQ=60)
             CCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAT                                                                                 <  SRR4302545.1346956/94‑1 (MQ=60)
             CCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAT                                                                                 >  SRR4302545.3913011/1‑94 (MQ=60)
             CCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAT                                                                                 >  SRR4302545.2919889/1‑94 (MQ=60)
             CCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAAT                                                                                 <  SRR4302545.3517803/94‑1 (MQ=60)
              CACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATT                                                                                >  SRR4302545.5818476/1‑94 (MQ=60)
              CACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATT                                                                                >  SRR4302545.5000467/1‑94 (MQ=60)
              CACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATT                                                                                >  SRR4302545.9888311/1‑94 (MQ=60)
               CCGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTC                                                                               <  SRR4302545.9339816/94‑1 (MQ=60)
               ACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTC                                                                               >  SRR4302545.8981961/1‑94 (MQ=60)
                  CGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGC                                                                            >  SRR4302545.4518356/1‑94 (MQ=60)
                  CGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGC                                                                            >  SRR4302545.6621409/1‑94 (MQ=60)
                   GCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCC                                                                           >  SRR4302545.797382/1‑94 (MQ=60)
                   GCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCC                                                                           >  SRR4302545.5886250/1‑94 (MQ=60)
                   GCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCC                                                                           >  SRR4302545.6582217/1‑94 (MQ=60)
                   GCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCC                                                                           >  SRR4302545.2846823/1‑94 (MQ=60)
                    CCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCT                                                                          >  SRR4302545.9485011/1‑94 (MQ=60)
                    CCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCT                                                                          <  SRR4302545.2477381/94‑1 (MQ=60)
                    CCTCCTTTCTCGATCAGCTTCTCGTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATCCCTTCGCCGGAGAGGATCTGGAATTCAGCCT                                                                          <  SRR4302545.6073342/94‑1 (MQ=60)
                     CTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTG                                                                         >  SRR4302545.2112776/1‑94 (MQ=60)
                     CTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTG                                                                         >  SRR4302545.1799842/1‑94 (MQ=60)
                     CTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTG                                                                         >  SRR4302545.6629601/1‑94 (MQ=60)
                     CTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTG                                                                         >  SRR4302545.5828449/1‑94 (MQ=60)
                      TGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGT                                                                        <  SRR4302545.2595899/94‑1 (MQ=60)
                      TGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGT                                                                        >  SRR4302545.1008396/1‑94 (MQ=60)
                       TCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTT                                                                       <  SRR4302545.8530328/94‑1 (MQ=60)
                       GCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTT                                                                       <  SRR4302545.8530321/94‑1 (MQ=60)
                       GCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTT                                                                       <  SRR4302545.7069561/94‑1 (MQ=60)
                        CTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTT                                                                      <  SRR4302545.4186948/94‑1 (MQ=60)
                        CTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTT                                                                      <  SRR4302545.696960/94‑1 (MQ=60)
                        CTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTT                                                                      <  SRR4302545.5032432/94‑1 (MQ=60)
                         TTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTA                                                                     <  SRR4302545.8412491/94‑1 (MQ=60)
                         TTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTA                                                                     <  SRR4302545.8412490/94‑1 (MQ=60)
                         TTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTA                                                                     >  SRR4302545.2376544/1‑94 (MQ=60)
                         TTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTA                                                                     <  SRR4302545.317661/94‑1 (MQ=60)
                         TTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTA                                                                     >  SRR4302545.611660/1‑94 (MQ=60)
                         TTTCTCGATCAGCTTCTATTTTACGCCCAGGACAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTA                                                                     >  SRR4302545.10712457/1‑94 (MQ=60)
                          TTCTCGATCAGTTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAA                                                                    <  SRR4302545.2058342/94‑1 (MQ=60)
                          TTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAA                                                                    <  SRR4302545.6611825/94‑1 (MQ=60)
                          TTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAA                                                                    >  SRR4302545.10341650/1‑94 (MQ=60)
                            CTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAAC                                                                  <  SRR4302545.8573602/94‑1 (MQ=60)
                            CTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAAC                                                                  <  SRR4302545.2957425/94‑1 (MQ=60)
                             TCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACG                                                                 <  SRR4302545.10056079/94‑1 (MQ=60)
                             TCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACG                                                                 <  SRR4302545.9573672/94‑1 (MQ=60)
                              CGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGG                                                                >  SRR4302545.6755896/1‑94 (MQ=60)
                               GATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGC                                                               >  SRR4302545.4766129/1‑94 (MQ=60)
                               GATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGC                                                               <  SRR4302545.3860450/94‑1 (MQ=60)
                               GATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGC                                                               <  SRR4302545.8904346/94‑1 (MQ=60)
                               GATCAGCTTCTCTTTTACGCCCAGGGCAACCAGGTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGGTCTGGAATTCAGACTGTTTAGACGGC                                                               >  SRR4302545.3729849/1‑94 (MQ=60)
                                GTCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCG                                                              <  SRR4302545.9420053/94‑1 (MQ=60)
                                ATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCG                                                              <  SRR4302545.1341740/94‑1 (MQ=60)
                                ATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCG                                                              <  SRR4302545.9420054/94‑1 (MQ=60)
                                ATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCG                                                              <  SRR4302545.10391084/94‑1 (MQ=60)
                                ATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCG                                                              >  SRR4302545.1983356/1‑94 (MQ=60)
                                 TCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGC                                                             >  SRR4302545.733155/1‑94 (MQ=60)
                                 TCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGC                                                             >  SRR4302545.3226595/1‑94 (MQ=60)
                                 TCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGC                                                             >  SRR4302545.9710120/1‑94 (MQ=60)
                                 TCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGC                                                             <  SRR4302545.4366514/94‑1 (MQ=60)
                                   AGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAG                                                           >  SRR4302545.6209614/1‑94 (MQ=60)
                                   AGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAG                                                           <  SRR4302545.44510/94‑1 (MQ=60)
                                   AGCTCCTCTTTCACGCCCAGGCCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAG                                                           <  SRR4302545.10073096/94‑1 (MQ=60)
                                    GCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGC                                                          <  SRR4302545.9137480/94‑1 (MQ=60)
                                     CTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCG                                                         <  SRR4302545.10636136/94‑1 (MQ=60)
                                     CTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCG                                                         >  SRR4302545.8364860/1‑94 (MQ=60)
                                     CTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCG                                                         <  SRR4302545.603560/94‑1 (MQ=60)
                                     CTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCG                                                         <  SRR4302545.5318703/94‑1 (MQ=60)
                                      TTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGA                                                        >  SRR4302545.8339209/1‑94 (MQ=60)
                                      CTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATCCCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGA                                                        <  SRR4302545.2875554/94‑1 (MQ=60)
                                       TCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGAT                                                       <  SRR4302545.7731557/94‑1 (MQ=60)
                                       TCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGAT                                                       <  SRR4302545.5369497/94‑1 (MQ=60)
                                        TTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATT                                                      >  SRR4302545.3936440/1‑94 (MQ=60)
                                        CTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATT                                                      >  SRR4302545.5321183/1‑94 (MQ=60)
                                        CTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATT                                                      >  SRR4302545.9745882/1‑94 (MQ=60)
                                         TCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTT                                                     >  SRR4302545.6201201/1‑94 (MQ=60)
                                         TCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTT                                                     <  SRR4302545.217020/94‑1 (MQ=60)
                                          CTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTT                                                    >  SRR4302545.8928441/1‑94 (MQ=60)
                                          CTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTT                                                    <  SRR4302545.1746767/94‑1 (MQ=60)
                                          CTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTT                                                    <  SRR4302545.7659657/94‑1 (MQ=60)
                                           TTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTG                                                   <  SRR4302545.3971914/94‑1 (MQ=60)
                                            TTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGT                                                  <  SRR4302545.2721554/94‑1 (MQ=60)
                                             TTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTT                                                 <  SRR4302545.1977587/94‑1 (MQ=60)
                                             TTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTT                                                 <  SRR4302545.1977586/94‑1 (MQ=60)
                                              TACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTC                                                <  SRR4302545.9297923/94‑1 (MQ=60)
                                              TACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTC                                                <  SRR4302545.9297617/94‑1 (MQ=60)
                                               ACGCCCAGGTCAACCAGTTCGCTGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCT                                               <  SRR4302545.9494316/94‑1 (MQ=60)
                                               ACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCT                                               <  SRR4302545.9488366/94‑1 (MQ=60)
                                                CGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTT                                              >  SRR4302545.952983/1‑94 (MQ=60)
                                                CGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTT                                              >  SRR4302545.6891892/1‑94 (MQ=60)
                                                CGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTT                                              >  SRR4302545.1639278/1‑94 (MQ=60)
                                                CGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTT                                              <  SRR4302545.7551466/94‑1 (MQ=60)
                                                CGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTT                                              >  SRR4302545.7448405/1‑94 (MQ=60)
                                                 GCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTC                                             >  SRR4302545.2968419/1‑94 (MQ=60)
                                                 GCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTC                                             >  SRR4302545.9284175/1‑94 (MQ=60)
                                                 GCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTA                                             >  SRR4302545.5365247/1‑94 (MQ=60)
                                                  CCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCA                                            <  SRR4302545.8189849/94‑1 (MQ=60)
                                                   CCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCAC                                           <  SRR4302545.5064226/94‑1 (MQ=60)
                                                   CCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCAC                                           >  SRR4302545.237944/1‑94 (MQ=60)
                                                     AGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCA                                         >  SRR4302545.4739446/1‑94 (MQ=60)
                                                     AGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCA                                         >  SRR4302545.5992117/1‑94 (MQ=60)
                                                     AGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCA                                         >  SRR4302545.1090750/1‑94 (MQ=60)
                                                       GTCAACCGGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACT                                       <  SRR4302545.3315740/94‑1 (MQ=60)
                                                       GTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACT                                       <  SRR4302545.6503432/94‑1 (MQ=60)
                                                       GTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACT                                       >  SRR4302545.7785468/1‑94 (MQ=60)
                                                       GTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACT                                       >  SRR4302545.4954864/1‑94 (MQ=60)
                                                        TCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTT                                      >  SRR4302545.2852796/1‑94 (MQ=60)
                                                         CAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTT                                     >  SRR4302545.4858883/1‑94 (MQ=60)
                                                         CAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTT                                     >  SRR4302545.8824742/1‑94 (MQ=60)
                                                          AACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTC                                    >  SRR4302545.589963/1‑94 (MQ=60)
                                                           ACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGGTTTTGTTCTTCACCACTTTCA                                   >  SRR4302545.1630567/1‑94 (MQ=60)
                                                           ACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCA                                   >  SRR4302545.2823655/1‑94 (MQ=60)
                                                           ACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCA                                   <  SRR4302545.8879906/94‑1 (MQ=60)
                                                           ACCAGTTCGCAGTAGAAGTTGATCCCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCA                                   <  SRR4302545.4157982/94‑1 (MQ=60)
                                                            CGAGTCCGCCTTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTGCTTCACCACTTTCCC                                  <  SRR4302545.4230864/94‑1 (MQ=60)
                                                            CCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAC                                  >  SRR4302545.10322905/1‑94 (MQ=60)
                                                            CCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAC                                  <  SRR4302545.1405878/94‑1 (MQ=60)
                                                            CCAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCAC                                  >  SRR4302545.8401414/1‑94 (MQ=60)
                                                             CAGTTCGCCGTAGAAGTTGATCCGTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACG                                 <  SRR4302545.3772726/94‑1 (MQ=60)
                                                             CAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACG                                 <  SRR4302545.9824283/94‑1 (MQ=60)
                                                             CAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACG                                 >  SRR4302545.180366/1‑94 (MQ=60)
                                                             CAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACG                                 >  SRR4302545.3032713/1‑94 (MQ=60)
                                                             CAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACG                                 <  SRR4302545.7322095/94‑1 (MQ=60)
                                                             CAGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACG                                 <  SRR4302545.3772724/94‑1 (MQ=60)
                                                              AGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGC                                >  SRR4302545.4239995/1‑94 (MQ=60)
                                                              AGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGC                                <  SRR4302545.1781151/94‑1 (MQ=60)
                                                              AGTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGC                                >  SRR4302545.5810167/1‑94 (MQ=60)
                                                               GTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCG                               >  SRR4302545.5831128/1‑94 (MQ=60)
                                                               GTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCG                               >  SRR4302545.3281689/1‑94 (MQ=60)
                                                               GTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCG                               <  SRR4302545.9642569/94‑1 (MQ=60)
                                                               GTTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCG                               <  SRR4302545.6705286/94‑1 (MQ=60)
                                                                gtcggCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGG                              <  SRR4302545.2074539/89‑1 (MQ=60)
                                                                TTCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGG                              <  SRR4302545.9512378/94‑1 (MQ=60)
                                                                 TCGCCGTAGAGGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGG                             <  SRR4302545.652772/94‑1 (MQ=60)
                                                                 TCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGG                             <  SRR4302545.7214123/94‑1 (MQ=60)
                                                                 TCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGG                             <  SRR4302545.6519576/94‑1 (MQ=60)
                                                                 TCGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGG                             <  SRR4302545.2611224/94‑1 (MQ=60)
                                                                  CGCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGT                            >  SRR4302545.8729690/1‑94 (MQ=60)
                                                                   GCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTT                           >  SRR4302545.10248761/1‑94 (MQ=60)
                                                                   GCCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTT                           <  SRR4302545.709599/94‑1 (MQ=60)
                                                                    CCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTT                          >  SRR4302545.10253005/1‑94 (MQ=60)
                                                                    CCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTT                          <  SRR4302545.9070468/94‑1 (MQ=60)
                                                                    CCGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTT                          >  SRR4302545.1151142/1‑94 (MQ=60)
                                                                     CGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTC                         <  SRR4302545.4896046/94‑1 (MQ=60)
                                                                     CGTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTC                         >  SRR4302545.3431624/1‑94 (MQ=60)
                                                                      GTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCG                        >  SRR4302545.7101105/1‑94 (MQ=60)
                                                                      GTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCG                        <  SRR4302545.10461880/94‑1 (MQ=60)
                                                                      GTAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCA                        <  SRR4302545.7558619/94‑1 (MQ=60)
                                                                       TAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGC                       <  SRR4302545.7744526/94‑1 (MQ=60)
                                                                       TAGAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGC                       >  SRR4302545.5081573/1‑94 (MQ=60)
                                                                         GAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTA                     <  SRR4302545.3939123/94‑1 (MQ=60)
                                                                         GAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTA                     <  SRR4302545.6345941/94‑1 (MQ=60)
                                                                         GAAGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTA                     >  SRR4302545.5537752/1‑94 (MQ=60)
                                                                          AAGTTGATACCTGCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTAC                    <  SRR4302545.9537710/94‑1 (MQ=60)
                                                                           AGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTCCC                   >  SRR4302545.6518795/1‑94 (MQ=60)
                                                                           AGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACC                   >  SRR4302545.8481066/1‑94 (MQ=60)
                                                                           AGTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACC                   >  SRR4302545.8481065/1‑94 (MQ=60)
                                                                            GTTGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCC                  <  SRR4302545.1486613/94‑1 (MQ=60)
                                                                              TGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCAC                <  SRR4302545.2574614/94‑1 (MQ=60)
                                                                              TGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCAC                >  SRR4302545.6387208/1‑94 (MQ=60)
                                                                              TGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCAC                >  SRR4302545.1043839/1‑94 (MQ=60)
                                                                              TGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCAC                >  SRR4302545.6138050/1‑94 (MQ=60)
                                                                              TGATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGCTTAAACGGCGCAGCGATTTTGTTCTGCACAACTTTCACGCGGGTGTCGCTACCTAC                >  SRR4302545.7030007/1‑94 (MQ=60)
                                                                               GATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACC               >  SRR4302545.6178043/1‑94 (MQ=60)
                                                                               GATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACC               <  SRR4302545.1886441/94‑1 (MQ=60)
                                                                                ATACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCA              <  SRR4302545.2219178/94‑1 (MQ=60)
                                                                                 TACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCAC             <  SRR4302545.80581/94‑1 (MQ=60)
                                                                                  ACCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACG            >  SRR4302545.8444665/1‑94 (MQ=60)
                                                                                   CCTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGT           >  SRR4302545.7568025/1‑94 (MQ=60)
                                                                                    CTTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTT          >  SRR4302545.1646869/1‑94 (MQ=60)
                                                                                     TTCGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTT         <  SRR4302545.4803888/94‑1 (MQ=60)
                                                                                       CGCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTC       <  SRR4302545.8967971/94‑1 (MQ=60)
                                                                                        GCCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCG      <  SRR4302545.425591/94‑1 (MQ=60)
                                                                                         CCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGC     >  SRR4302545.10128877/1‑94 (MQ=60)
                                                                                         CCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGC     >  SRR4302545.10169198/1‑94 (MQ=60)
                                                                                         CCGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGC     <  SRR4302545.5028660/94‑1 (MQ=60)
                                                                                          CGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGCC    >  SRR4302545.555881/1‑94 (MQ=60)
                                                                                          CGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGCC    <  SRR4302545.10683365/94‑1 (MQ=60)
                                                                                          CGGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGCC    >  SRR4302545.4239424/1‑94 (MQ=60)
                                                                                           GGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCCCCACGTTTTCGCCC   >  SRR4302545.6936249/1‑94 (MQ=60)
                                                                                           GGAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGCCC   >  SRR4302545.984521/1‑94 (MQ=60)
                                                                                            GAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGCCCT  <  SRR4302545.5359326/94‑1 (MQ=60)
                                                                                            |                                                                                             
CTTTGTAGCTGTACCACGCGCCTGCTTTCTCGATCAGCTTCTCTTTTACGCCCAGGTCAACCAGTTCGCCGTAGAAGTTGATACCTTCGCCGTAGAGGATCTGGAATTCAGCCTGTTTAAACGGCGCAGCGATTTTGTTCTTCACCACTTTCACGCGGGTTTCGCTACCCACCACGTTTTCGCCCT  >  NC_000913/2820907‑2821092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑