Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,158,202 +C coding (961/975 nt) ygiRQ ← Fe‑S oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,158,2011.C99.1% 1567.3 / 1.8 459coding (962/975 nt)ygiRQFe‑S oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/4);  new base C (193/262);  total (193/266)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 518 total aligned reads displayed.

AACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG  >  NC_000913/3158108‑3158294
                                                                                              |                                                                                             
 aCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑c                                                                                              <  1:7660930/94‑1 (MQ=255)
 aCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑c                                                                                              <  1:4864648/94‑1 (MQ=255)
  cGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cc                                                                                             <  1:2720749/94‑2 (MQ=255)
   gCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cct                                                                                            <  1:5521746/94‑3 (MQ=255)
   gCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cct                                                                                            >  1:2544216/1‑92 (MQ=255)
    cTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctg                                                                                           >  1:8927209/1‑91 (MQ=255)
    cTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctg                                                                                           <  1:7420243/94‑4 (MQ=255)
     tGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctgg                                                                                          >  2:10327010/1‑90 (MQ=255)
     tGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctgg                                                                                          <  1:6347000/94‑5 (MQ=255)
     tGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctgg                                                                                          >  1:2302093/1‑90 (MQ=255)
       tCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggt                                                                                         <  1:9113486/94‑1 (MQ=255)
       tCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggt                                                                                         <  1:8491785/94‑1 (MQ=255)
       gccAGCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATCGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggt                                                                                         <  2:4802804/93‑1 (MQ=255)
        ccATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggtt                                                                                        <  2:2777949/94‑1 (MQ=255)
         cATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttg                                                                                       <  1:125955/94‑1 (MQ=255)
          aTCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggctgg                                                                                      >  1:10347361/1‑94 (MQ=255)
            ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggtt                                                                                    >  1:2240151/1‑94 (MQ=255)
            ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggtt                                                                                    >  2:8442893/1‑94 (MQ=255)
            ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggtt                                                                                    <  2:4676644/94‑1 (MQ=255)
             tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGttt                                                                                   <  1:10008147/94‑1 (MQ=255)
                tCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgt                                                                                <  2:8599932/94‑1 (MQ=255)
                  ggTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtgg                                                                              <  2:1049842/94‑1 (MQ=255)
                     aGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                           >  2:7881095/1‑94 (MQ=255)
                     aGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                           <  1:6999472/94‑1 (MQ=255)
                      gAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCa                                                                          >  1:9881748/1‑94 (MQ=255)
                         aGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGaa                                                                       >  1:3336527/1‑94 (MQ=255)
                           cGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                                     <  2:1112609/94‑1 (MQ=255)
                           cGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                                     >  2:9437167/1‑94 (MQ=255)
                            ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                                    <  2:513927/94‑1 (MQ=255)
                            ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                                    >  1:6513353/1‑94 (MQ=255)
                            ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                                    >  2:732135/1‑94 (MQ=255)
                            ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                                    >  2:2992844/1‑94 (MQ=255)
                             gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcg                                                                   <  2:6024346/94‑1 (MQ=255)
                             gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcg                                                                   <  1:7283004/94‑1 (MQ=255)
                             gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcg                                                                   <  2:2163267/94‑1 (MQ=255)
                              aaTAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgc                                                                  >  2:5423449/1‑94 (MQ=255)
                              aaTAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgc                                                                  <  2:4135754/94‑1 (MQ=255)
                                 aGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGag                                                               >  2:2319236/1‑93 (MQ=255)
                                 aGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGaa                                                               <  2:373725/94‑1 (MQ=255)
                                 aGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGaa                                                               >  2:7722327/1‑94 (MQ=255)
                                 aGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGaa                                                               >  1:1693230/1‑94 (MQ=255)
                                 aGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGCTTCGTGGTGCAGAATACGCGaa                                                               >  1:3010496/1‑94 (MQ=255)
                                  gCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGGGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg                                                              <  2:9214535/94‑1 (MQ=255)
                                  gCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg                                                              <  1:4407144/94‑1 (MQ=255)
                                   cAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc                                                             <  2:7056358/94‑1 (MQ=255)
                                    aGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGct                                                            >  1:5714875/1‑93 (MQ=255)
                                     ggCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAt                                                           <  2:2048187/94‑1 (MQ=255)
                                      gCGGGTTTGTCCCCCCATAGGGGTTGCCGTTTTTTTGGATCAATGCGCGGGCCCAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg                                                          <  2:6671004/94‑1 (MQ=255)
                                       cGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATgg                                                         >  2:6345507/1‑94 (MQ=255)
                                       cGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATgg                                                         <  1:1010881/94‑1 (MQ=255)
                                       cGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATgg                                                         <  1:8284573/94‑1 (MQ=255)
                                        gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATggg                                                        <  2:144369/94‑1 (MQ=255)
                                         ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGc                                                       <  2:6390142/94‑1 (MQ=255)
                                         ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGc                                                       <  2:8315264/94‑1 (MQ=255)
                                             gATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTAc                                                   >  1:600166/1‑94 (MQ=255)
                                             gATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTAc                                                   >  1:2035465/1‑94 (MQ=255)
                                               tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAg                                                 >  1:3144489/1‑94 (MQ=255)
                                               tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAg                                                 <  2:9542041/94‑1 (MQ=255)
                                               tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAg                                                 <  2:4370244/94‑1 (MQ=255)
                                                ccACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGc                                                <  1:2145062/94‑1 (MQ=255)
                                                 cacacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCa                                               <  1:9711075/94‑1 (MQ=255)
                                                  acacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCAc                                              >  2:8978857/1‑94 (MQ=255)
                                                   ctcaTAGCGTGCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAATCATGGGCGTACAGCACt                                             <  1:1906667/92‑1 (MQ=255)
                                                   cacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACt                                             <  1:2637552/94‑1 (MQ=255)
                                                   cacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACt                                             >  2:1890081/1‑94 (MQ=255)
                                                    acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACtt                                            <  1:7103554/94‑1 (MQ=255)
                                                     caTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACttt                                           <  2:3405016/94‑1 (MQ=255)
                                                     caTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACttt                                           <  1:7821820/94‑1 (MQ=255)
                                                      aTAGCGGTCTCCGTGTTTTTGCATCAATGCACGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                          <  1:3759296/94‑1 (MQ=255)
                                                      aTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                          <  2:5339393/94‑1 (MQ=255)
                                                      aTAGCGGTCGCCATGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                          <  1:9385491/94‑1 (MQ=255)
                                                         gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCg                                       <  1:6705464/94‑1 (MQ=255)
                                                         gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCg                                       <  1:4052026/94‑1 (MQ=255)
                                                           ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGACGCATGGGCGTACAGCACTTTATCGcc                                     >  2:5933915/1‑94 (MQ=255)
                                                            gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGccc                                    >  2:10158228/1‑94 (MQ=255)
                                                             tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCt                                   <  1:10581656/94‑1 (MQ=255)
                                                               gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTc                                 >  2:5741284/1‑94 (MQ=255)
                                                                   tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGTCCTTCACtt                             >  1:10243802/1‑94 (MQ=255)
                                                                   tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTccgtt                             >  2:6554686/1‑90 (MQ=255)
                                                                    gtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACttt                            <  2:6936970/94‑1 (MQ=255)
                                                                      ttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTtct                          <  2:2518290/94‑1 (MQ=255)
                                                                      ttttGCATCAATGCGCGGCCACAGCCTGGGTTGGTTTCTTGTGGAGGAATACGCGAACAATGGGCGAACAGCACTTAATCGCCCTCCACTTcct                          >  2:3502402/1‑94 (MQ=255)
                                                                       tttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTtctc                         <  1:6886171/94‑1 (MQ=255)
                                                                        ttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCg                        <  1:8734598/94‑1 (MQ=255)
                                                                          gCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaa                      >  2:9707151/1‑94 (MQ=255)
                                                                          gCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaa                      <  1:8876496/94‑1 (MQ=255)
                                                                           cATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa                     >  2:3249574/1‑94 (MQ=255)
                                                                           cATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa                     <  1:8363844/94‑1 (MQ=255)
                                                                            aTCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaag                    >  1:453699/1‑94 (MQ=255)
                                                                               aaTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaagaag                 >  2:4993931/1‑94 (MQ=255)
                                                                               aaTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaagaag                 <  2:8809957/94‑1 (MQ=255)
                                                                               aaTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaagaag                 <  2:2404631/94‑1 (MQ=255)
                                                                               aaTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaagaag                 >  2:166786/1‑94 (MQ=255)
                                                                                aTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAgg                <  1:4255103/94‑1 (MQ=255)
                                                                                  gcgcGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgca              <  1:3624563/94‑1 (MQ=255)
                                                                                  gcgcGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgca              <  2:7523606/94‑1 (MQ=255)
                                                                                   cgcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgcag             <  2:7165265/94‑1 (MQ=255)
                                                                                    gcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgcagc            <  2:8737682/94‑1 (MQ=255)
                                                                                       ggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAac         <  2:559552/94‑1 (MQ=255)
                                                                                       ggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAac         <  2:3900433/94‑1 (MQ=255)
                                                                                              |                                                                                             
AACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG  >  NC_000913/3158108‑3158294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 267 total aligned reads displayed.
GAATCGGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAGCTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCGC  >  NC_000913/3166842‑3167024
                                                                                             |                                                                                         
GAATCGGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAC                                                                                           >  SRR4302545.5159911/1‑94 (MQ=60)
GAATCGGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAC                                                                                           >  SRR4302545.1556154/1‑94 (MQ=60)
GAATCGGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAC                                                                                           <  SRR4302545.7573844/94‑1 (MQ=60)
GAATCGGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAC                                                                                           <  SRR4302545.8251073/94‑1 (MQ=60)
GAATCGGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAC                                                                                           <  SRR4302545.8620726/94‑1 (MQ=60)
GAATCGGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAC                                                                                           >  SRR4302545.5159910/1‑94 (MQ=60)
     GGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGC                                                                                      <  SRR4302545.3413701/94‑1 (MQ=60)
      GTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGTCCCATACCGTGTGTGAAATATCGGCCAGCTCGCCAACGTGGCG                                                                                     >  SRR4302545.9667614/1‑94 (MQ=60)
      GTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCG                                                                                     <  SRR4302545.4655935/94‑1 (MQ=60)
      GTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCG                                                                                     >  SRR4302545.7175626/1‑94 (MQ=60)
      GTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCG                                                                                     <  SRR4302545.4655936/94‑1 (MQ=60)
      GTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCG                                                                                     >  SRR4302545.2130632/1‑94 (MQ=60)
      GTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCG                                                                                     <  SRR4302545.2981955/94‑1 (MQ=60)
       TGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGG                                                                                    >  SRR4302545.6264012/1‑94 (MQ=60)
       TGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGG                                                                                    <  SRR4302545.7500810/94‑1 (MQ=60)
       TGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGG                                                                                    >  SRR4302545.10288345/1‑94 (MQ=60)
       TGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGG                                                                                    <  SRR4302545.8640817/94‑1 (MQ=60)
       TGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGG                                                                                    <  SRR4302545.4940751/94‑1 (MQ=60)
        GCTTTGCGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGG                                                                                   >  SRR4302545.5698033/1‑94 (MQ=60)
         CTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGC                                                                                  <  SRR4302545.3710052/94‑1 (MQ=60)
         CTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGC                                                                                  >  SRR4302545.230848/1‑94 (MQ=60)
         CTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGC                                                                                  >  SRR4302545.1584601/1‑94 (MQ=60)
         CTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGC                                                                                  >  SRR4302545.3635327/1‑94 (MQ=60)
          TTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCACACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGGC                                                                                 >  SRR4302545.4303574/1‑94 (MQ=60)
           TTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGGCCCATTACATGTGTGAAATATCGTGTAGCTCGCCAACGTGGCGGGCCA                                                                                >  SRR4302545.6507611/1‑94 (MQ=60)
           TTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCA                                                                                <  SRR4302545.8468429/94‑1 (MQ=60)
           TTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCA                                                                                <  SRR4302545.10827366/94‑1 (MQ=60)
           TTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCA                                                                                >  SRR4302545.2523243/1‑94 (MQ=60)
           TTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCA                                                                                <  SRR4302545.9049998/94‑1 (MQ=60)
            TACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCCACGTGGCGGGCCAC                                                                               >  SRR4302545.6604467/1‑94 (MQ=60)
             ACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACG                                                                              <  SRR4302545.8080126/94‑1 (MQ=60)
             ACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGCGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACG                                                                              >  SRR4302545.5087079/1‑94 (MQ=60)
              CGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGG                                                                             >  SRR4302545.7546031/1‑94 (MQ=60)
               GCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGGCCCGGC                                                                            >  SRR4302545.252564/1‑94 (MQ=60)
                CATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCA                                                                           <  SRR4302545.1306568/94‑1 (MQ=60)
                CATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCA                                                                           >  SRR4302545.1782383/1‑94 (MQ=60)
                CATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCA                                                                           >  SRR4302545.10298657/1‑94 (MQ=60)
                 ATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCAT                                                                          >  SRR4302545.5096800/1‑94 (MQ=60)
                 ATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCAT                                                                          <  SRR4302545.2311299/94‑1 (MQ=60)
                 ATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCAT                                                                          >  SRR4302545.10225038/1‑94 (MQ=60)
                  TTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCTTCCATCTCGCCAACGTTTCGGGCCACGGCATA                                                                         <  SRR4302545.1484406/94‑1 (MQ=60)
                  TTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATA                                                                         <  SRR4302545.8285907/94‑1 (MQ=60)
                  TTTTCTCGCCGCTTGCAGGCAGCCAGTGACGAATGATGCACCATTCCGTGTGTAAAATATCGTCCAGCTCGCAAACGTGGCGGGCCACGGCATA                                                                         <  SRR4302545.8285908/94‑1 (MQ=60)
                   TTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAA                                                                        >  SRR4302545.6020635/1‑94 (MQ=60)
                    TTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAAC                                                                       <  SRR4302545.9259110/94‑1 (MQ=60)
                    TTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAAC                                                                       <  SRR4302545.3003092/94‑1 (MQ=60)
                      CTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGT                                                                     >  SRR4302545.5773152/1‑94 (MQ=60)
                      CTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGT                                                                     >  SRR4302545.10774571/1‑94 (MQ=60)
                       TCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTC                                                                    <  SRR4302545.3422533/94‑1 (MQ=60)
                        CGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCC                                                                   <  SRR4302545.6984247/94‑1 (MQ=60)
                        CGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCC                                                                   >  SRR4302545.7564891/1‑94 (MQ=60)
                        CGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCC                                                                   <  SRR4302545.5389760/94‑1 (MQ=60)
                         GCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCA                                                                  <  SRR4302545.6325742/94‑1 (MQ=60)
                         GCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCA                                                                  >  SRR4302545.4962958/1‑94 (MQ=60)
                          CCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAC                                                                 <  SRR4302545.5629307/94‑1 (MQ=60)
                          CCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAC                                                                 >  SRR4302545.7284043/1‑94 (MQ=60)
                          CCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAC                                                                 <  SRR4302545.8028954/94‑1 (MQ=60)
                          CCGCTTGCAGGCAGCCAGTGGCGAATGATGACCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAC                                                                 >  SRR4302545.7649645/1‑94 (MQ=60)
                          CCCCTGGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAC                                                                 <  SRR4302545.8047085/94‑1 (MQ=60)
                           CGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACC                                                                <  SRR4302545.5786052/94‑1 (MQ=60)
                           CGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACC                                                                >  SRR4302545.3105788/1‑94 (MQ=60)
                           CGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACA                                                                <  SRR4302545.4163359/94‑1 (MQ=60)
                           CGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACATCCACC                                                                >  SRR4302545.2916270/1‑94 (MQ=60)
                            GCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCG                                                               <  SRR4302545.4089470/94‑1 (MQ=60)
                            GCGTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTTAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCG                                                               <  SRR4302545.3451151/94‑1 (MQ=60)
                             CTTGCAGGCAGCCAGTGGCGAATGATGCTCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCCCCGG                                                              <  SRR4302545.3577172/94‑1 (MQ=60)
                             CTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGG                                                              <  SRR4302545.5403329/94‑1 (MQ=60)
                                GCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAA                                                           <  SRR4302545.5697410/94‑1 (MQ=60)
                                GCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAA                                                           >  SRR4302545.6857209/1‑94 (MQ=60)
                                GCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAA                                                           <  SRR4302545.9332567/94‑1 (MQ=60)
                                 CAGGCAGCGAGTGGAGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAG                                                          <  SRR4302545.903583/94‑1 (MQ=60)
                                 CAGGCAGCCAGTGGCGAATGATGCCCCATATCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAG                                                          <  SRR4302545.10653836/94‑1 (MQ=60)
                                 CAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAG                                                          <  SRR4302545.3710779/94‑1 (MQ=60)
                                 CAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAG                                                          >  SRR4302545.1665975/1‑94 (MQ=60)
                                  AGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCCCGGCATAACGTCCACCGGTAAGT                                                         >  SRR4302545.5837372/1‑94 (MQ=60)
                                   GGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTT                                                        >  SRR4302545.2153303/1‑94 (MQ=60)
                                   GGCAGCCAGTGGCGAATGATACCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTT                                                        >  SRR4302545.1402066/1‑94 (MQ=60)
                                    GCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTC                                                       >  SRR4302545.2843759/1‑94 (MQ=60)
                                     CAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCTGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCA                                                      <  SRR4302545.6484378/94‑1 (MQ=60)
                                     CAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCCCGGCATAAAGCCCACCGGGAAGTTgc                                                      >  SRR4302545.8189136/1‑92 (MQ=60)
                                     CAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCC                                                      >  SRR4302545.3623050/1‑94 (MQ=60)
                                      AGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCAC                                                     >  SRR4302545.9978663/1‑94 (MQ=60)
                                      AGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCAC                                                     >  SRR4302545.1753980/1‑94 (MQ=60)
                                      AGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCAC                                                     >  SRR4302545.2389305/1‑94 (MQ=60)
                                      AGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCAC                                                     >  SRR4302545.10654333/1‑94 (MQ=60)
                                        CCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCA                                                   >  SRR4302545.5146663/1‑94 (MQ=60)
                                         CAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAT                                                  <  SRR4302545.7951530/94‑1 (MQ=60)
                                         CAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAT                                                  <  SRR4302545.4963493/94‑1 (MQ=60)
                                         CAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAT                                                  <  SRR4302545.5921/94‑1 (MQ=60)
                                         CAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCAT                                                  >  SRR4302545.6856231/1‑94 (MQ=60)
                                          AGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATT                                                 <  SRR4302545.6733130/94‑1 (MQ=60)
                                          AGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGGAAGTTCACCATT                                                 >  SRR4302545.6047564/1‑94 (MQ=60)
                                           GTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTG                                                <  SRR4302545.3173903/94‑1 (MQ=60)
                                            TGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGC                                               >  SRR4302545.2133752/1‑94 (MQ=60)
                                            TGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGC                                               >  SRR4302545.8804061/1‑94 (MQ=60)
                                            TGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGC                                               <  SRR4302545.5766045/94‑1 (MQ=60)
                                             GGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCT                                              >  SRR4302545.1440460/1‑94 (MQ=60)
                                             GGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCT                                              <  SRR4302545.1055450/94‑1 (MQ=60)
                                               CGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCGACCGGTAAGTTCACCATTGCTCA                                            >  SRR4302545.7805955/1‑94 (MQ=60)
                                               CGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCC                                            >  SRR4302545.2753608/1‑94 (MQ=60)
                                               CGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCA                                            <  SRR4302545.9637120/94‑1 (MQ=60)
                                               CGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCAACGGTAAGTTCACCATTGCTCA                                            >  SRR4302545.3309676/1‑94 (MQ=60)
                                                GAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGCTCACCATTGCTCAC                                           >  SRR4302545.4222873/1‑94 (MQ=60)
                                                GAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGGAAGTTCACCATTGCTCAC                                           >  SRR4302545.3035744/1‑94 (MQ=60)
                                                GAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCAACGCATAACGTCCACCCGTAAGGTTACCATTGCTCAC                                           >  SRR4302545.6315271/1‑94 (MQ=60)
                                                 AATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACA                                          <  SRR4302545.4398579/94‑1 (MQ=60)
                                                 AATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACA                                          <  SRR4302545.697106/94‑1 (MQ=60)
                                                  ATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACAC                                         >  SRR4302545.3176060/1‑94 (MQ=60)
                                                  ATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACAC                                         <  SRR4302545.543787/94‑1 (MQ=60)
                                                   TGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACC                                        <  SRR4302545.527060/94‑1 (MQ=60)
                                                   TGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCCGTAAGTTCACCATTGCTCACACC                                        >  SRR4302545.4881895/1‑94 (MQ=60)
                                                    GATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCA                                       >  SRR4302545.5387040/1‑94 (MQ=60)
                                                    GATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCA                                       <  SRR4302545.5803170/94‑1 (MQ=60)
                                                      TGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATA                                     >  SRR4302545.2693383/1‑94 (MQ=60)
                                                       GCCCCATGCCGTGTGTGAAATATCGTCCATCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAA                                    <  SRR4302545.3073661/94‑1 (MQ=60)
                                                       GCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAA                                    <  SRR4302545.6608153/94‑1 (MQ=60)
                                                        CCCCATACCGTGTGTGAAATATCGTTCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAAC                                   <  SRR4302545.1178964/94‑1 (MQ=60)
                                                        CCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAAG                                   >  SRR4302545.936180/1‑94 (MQ=60)
                                                        CCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAAC                                   >  SRR4302545.7952040/1‑94 (MQ=60)
                                                         CCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACG                                  <  SRR4302545.7149518/94‑1 (MQ=60)
                                                          CCATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGA                                 <  SRR4302545.3796666/94‑1 (MQ=60)
                                                           CATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGGCACGGCATAACGTCCAGCGGGAAGTGCACCATTGCTCccaccctagcgct                                >  SRR4302545.1344815/1‑81 (MQ=60)
                                                           CATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAT                                >  SRR4302545.317529/1‑94 (MQ=60)
                                                           CATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAT                                >  SRR4302545.1344816/1‑94 (MQ=60)
                                                           CATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGAT                                <  SRR4302545.702314/94‑1 (MQ=60)
                                                           CATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACAACATAACGAT                                >  SRR4302545.10012014/1‑94 (MQ=60)
                                                           CATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGGTTACCATTGCTCACACCCTCACGAT                                >  SRR4302545.5916734/1‑94 (MQ=60)
                                                            ATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATT                               >  SRR4302545.920813/1‑94 (MQ=60)
                                                            ATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATT                               >  SRR4302545.2705312/1‑94 (MQ=60)
                                                            ATACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGAATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATT                               >  SRR4302545.4759560/1‑94 (MQ=60)
                                                             TACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTA                              >  SRR4302545.6973000/1‑94 (MQ=60)
                                                             TACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTA                              <  SRR4302545.5381255/94‑1 (MQ=60)
                                                              ACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTAT                             <  SRR4302545.7284835/94‑1 (MQ=60)
                                                              ACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTAT                             <  SRR4302545.9213605/94‑1 (MQ=60)
                                                              ACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTAT                             >  SRR4302545.1664392/1‑94 (MQ=60)
                                                              ACCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTAT                             <  SRR4302545.7465522/94‑1 (MQ=60)
                                                               CCGTGTGTGAAATATCGTCCAGCTCGCCACCGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCCCACCATAACGAATATC                            >  SRR4302545.4809230/1‑94 (MQ=60)
                                                               CCGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATC                            <  SRR4302545.10548790/94‑1 (MQ=60)
                                                                CGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCG                           <  SRR4302545.6048573/94‑1 (MQ=60)
                                                                CGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCG                           <  SRR4302545.876508/94‑1 (MQ=60)
                                                                CGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCG                           >  SRR4302545.601601/1‑94 (MQ=60)
                                                                CGTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCG                           >  SRR4302545.452846/1‑94 (MQ=60)
                                                                 GTTTGTGAAATATCGTCCAGCTCGCCTGCGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGG                          <  SRR4302545.5863646/94‑1 (MQ=60)
                                                                 GTGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGG                          <  SRR4302545.5362999/94‑1 (MQ=60)
                                                                  TGTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACAATTGCTCACACCATAACGATTATCGGG                         >  SRR4302545.5613545/1‑94 (MQ=60)
                                                                   GTGTGAAATATCGTCTAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGA                        <  SRR4302545.1648437/94‑1 (MQ=60)
                                                                   GTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGA                        <  SRR4302545.8881093/94‑1 (MQ=60)
                                                                   GTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGA                        <  SRR4302545.4192617/94‑1 (MQ=60)
                                                                   GTGTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGA                        <  SRR4302545.5861741/94‑1 (MQ=60)
                                                                     GTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAAT                      >  SRR4302545.3899404/1‑94 (MQ=60)
                                                                     GTGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAAT                      <  SRR4302545.7301136/94‑1 (MQ=60)
                                                                     GTGAAATATCGACCAGCTCGCCAACATGGCGGGCGAAGGCATAACACCCACCTGTAAGGTCAGCATTGCTCACACCATAACGATTATatggcat                      >  SRR4302545.5545422/1‑87 (MQ=30)
                                                                      TGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATC                     >  SRR4302545.5557569/1‑94 (MQ=60)
                                                                      TGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATC                     >  SRR4302545.1748584/1‑94 (MQ=60)
                                                                      TGAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATC                     <  SRR4302545.4861643/94‑1 (MQ=60)
                                                                       GAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCG                    <  SRR4302545.8440763/94‑1 (MQ=60)
                                                                       GAAATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCG                    <  SRR4302545.7202398/94‑1 (MQ=60)
                                                                       GAAATATCGTCCAGCTCGCCAACGGGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCG                    <  SRR4302545.7202397/94‑1 (MQ=60)
                                                                         AATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGT                  <  SRR4302545.10015226/94‑1 (MQ=60)
                                                                          ATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTT                 >  SRR4302545.2277899/1‑94 (MQ=60)
                                                                          ATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTT                 <  SRR4302545.6474991/94‑1 (MQ=60)
                                                                          ATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTT                 <  SRR4302545.3768545/94‑1 (MQ=60)
                                                                          ATATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTT                 >  SRR4302545.2990458/1‑94 (MQ=60)
                                                                          ATATCCGCCAGCTCGCCAACGAGGCGGGGCACGGCATAACGTACAACGGCAAGTTCACCATTGCTCACACCCTAACGATTATCGTGAATCGGTT                 >  SRR4302545.1675671/1‑94 (MQ=38)
                                                                           TATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTC                <  SRR4302545.9876777/94‑1 (MQ=60)
                                                                           TATCGTCCAGCTCGCCAACGTGGAGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTC                <  SRR4302545.6008812/94‑1 (MQ=60)
                                                                            ATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCG               <  SRR4302545.8021401/94‑1 (MQ=60)
                                                                            ATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCG               >  SRR4302545.4250704/1‑94 (MQ=60)
                                                                            ATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCG               >  SRR4302545.4867049/1‑94 (MQ=60)
                                                                            ATCGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCG               >  SRR4302545.3280928/1‑94 (MQ=60)
                                                                              CGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCT             <  SRR4302545.8085800/94‑1 (MQ=60)
                                                                              CGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCT             >  SRR4302545.2811028/1‑94 (MQ=60)
                                                                              CGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCT             >  SRR4302545.900098/1‑94 (MQ=60)
                                                                              CGTCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTACACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCT             <  SRR4302545.2751790/94‑1 (MQ=60)
                                                                                TCCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAA           >  SRR4302545.10403763/1‑94 (MQ=60)
                                                                                 CCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAAC          <  SRR4302545.7550022/94‑1 (MQ=60)
                                                                                 CCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAAC          <  SRR4302545.4112996/94‑1 (MQ=60)
                                                                                 CCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAAC          >  SRR4302545.9338650/1‑94 (MQ=60)
                                                                                 CCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAAC          >  SRR4302545.6296456/1‑94 (MQ=60)
                                                                                 CCAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAAC          <  SRR4302545.7220559/94‑1 (MQ=60)
                                                                                 CCAGCTCGCCAACGTGGCGGGCCACGCCATAACGTCCACCGATAAGTTCACCATTGCTCACACCATAGCGATTAACGGGAATCGGTTCGCTAAC          <  SRR4302545.1225444/94‑1 (MQ=60)
                                                                                  CAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACG         >  SRR4302545.7481458/1‑94 (MQ=60)
                                                                                  CAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACG         >  SRR4302545.2946698/1‑94 (MQ=60)
                                                                                  CAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACG         <  SRR4302545.3519880/94‑1 (MQ=60)
                                                                                  CAGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACC         >  SRR4302545.9782487/1‑94 (MQ=60)
                                                                                   CGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGC        <  SRR4302545.10003821/94‑1 (MQ=60)
                                                                                   AGCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGC        >  SRR4302545.4771546/1‑94 (MQ=60)
                                                                                    GCTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCT       <  SRR4302545.4633284/94‑1 (MQ=60)
                                                                                     CTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTG      <  SRR4302545.111060/94‑1 (MQ=60)
                                                                                     CTCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAAAGCTG      >  SRR4302545.8610360/1‑94 (MQ=60)
                                                                                     CTCGCCAAAGTGGCGGGCCACGGCATAACCTGCACCGGTAAGATCACCATTGCTCACACCATGACGATTATCGGacataggcttggcaacggtc      >  SRR4302545.851388/1‑74 (MQ=52)
                                                                                      TCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGC     >  SRR4302545.1939941/1‑94 (MQ=60)
                                                                                      TCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGC     >  SRR4302545.136655/1‑94 (MQ=60)
                                                                                      TCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGC     >  SRR4302545.1773474/1‑94 (MQ=60)
                                                                                      TCGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGC     >  SRR4302545.6616572/1‑94 (MQ=60)
                                                                                       CGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCC    >  SRR4302545.1929995/1‑94 (MQ=60)
                                                                                       CGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCC    <  SRR4302545.7640174/94‑1 (MQ=60)
                                                                                       CGCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCC    <  SRR4302545.1073402/94‑1 (MQ=60)
                                                                                        GCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCG   >  SRR4302545.3866612/1‑94 (MQ=60)
                                                                                        GCCAACGTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCG   <  SRR4302545.2690971/94‑1 (MQ=60)
                                                                                             |                                                                                         
GAATCGGTGCTTTACGCATTTTCTCGCCGCTTGCAGGCAGCCAGTGGCGAATGATGCCCCATACCGTGTGTGAAATATCGTCCAGCTCGCCAAGCTGGCGGGCCACGGCATAACGTCCACCGGTAAGTTCACCATTGCTCACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCGC  >  NC_000913/3166842‑3167024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑