Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,174,443 T→C H136R (CAC→CGC)  icc ← Icc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,174,4430TC99.5% 1410.7 / ‑3.7 409H136R (CAC→CGC) iccIcc
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (200/207);  total (200/209)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 427 total aligned reads displayed.

ATGATGATGCAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCT  >  NC_000913/3174352‑3174533
                                                                                           |                                                                                          
 tgatgatgCAGCAGCAGCAACGTATGGCGTTCTGGCTCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACcgcgcg                                                                                         <  2:7598270/94‑1 (MQ=255)
   atgatgCAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGc                                                                                       >  2:7073115/1‑94 (MQ=255)
   atgatgCAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGc                                                                                       <  2:10400678/94‑1 (MQ=255)
     gatgCAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCAc                                                                                     >  2:5321798/1‑94 (MQ=255)
     gatgCAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCAc                                                                                     <  2:27946/94‑1 (MQ=255)
        gcagcagcagcaACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGcc                                                                                  >  2:9795107/1‑94 (MQ=255)
         cagcagcagcaACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCa                                                                                 >  1:9474694/1‑94 (MQ=255)
          agcagcagcaACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACTCCaa                                                                                >  2:339277/1‑94 (MQ=255)
           gcagcagcaACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCaaa                                                                               <  1:3426486/94‑1 (MQ=255)
            cagcagcaACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAac                                                                              >  1:2043222/1‑94 (MQ=255)
             agcagcaACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAaca                                                                             >  2:4477886/1‑94 (MQ=255)
             agcagcaACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTCCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAaca                                                                             <  2:2469609/94‑1 (MQ=255)
                agcaACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCt                                                                          <  1:10444624/94‑1 (MQ=255)
                  caACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTgg                                                                        <  2:6018352/94‑1 (MQ=255)
                  caACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTgg                                                                        <  2:4749141/94‑1 (MQ=255)
                  caACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTgg                                                                        >  1:5620766/1‑94 (MQ=255)
                   aaCGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCAAGCGAAACACCgggt                                                                       >  1:2502206/1‑93 (MQ=255)
                    aCGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCt                                                                      <  2:6260051/94‑1 (MQ=255)
                     cGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTa                                                                     <  1:5317064/94‑1 (MQ=255)
                     cGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTa                                                                     >  1:3196537/1‑94 (MQ=255)
                      gTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTAt                                                                    >  2:9518341/1‑94 (MQ=255)
                      gTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTAt                                                                    >  1:1571777/1‑94 (MQ=255)
                       tATGGCGTTCTGGCGCATCTGCCAGTTTACGTTCCAGCCACTGAAGGTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTTGCTATc                                                                   >  1:6266340/1‑94 (MQ=255)
                         tGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCa                                                                 <  2:10065758/94‑1 (MQ=255)
                          ggCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAg                                                                <  2:5031523/94‑1 (MQ=255)
                            cGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCa                                                              <  2:3839565/94‑1 (MQ=255)
                             gTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCTGCACGCCAAACACCTGGCTATCCCGCac                                                             >  2:2651693/1‑93 (MQ=255)
                             gTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCaa                                                             >  2:2170088/1‑94 (MQ=255)
                              ttCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAAc                                                            <  1:7065029/94‑1 (MQ=255)
                                cTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAg                                                          <  1:8342562/94‑1 (MQ=255)
                                cTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAg                                                          <  2:3757460/94‑1 (MQ=255)
                                 tGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAgg                                                         <  1:7393869/94‑1 (MQ=255)
                                   gcgcATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGAt                                                       <  1:7226552/94‑1 (MQ=255)
                                   gcgcACCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGAt                                                       <  1:4610787/94‑1 (MQ=255)
                                    cgcATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGAtt                                                      >  2:2033845/1‑94 (MQ=255)
                                     gcATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGAttt                                                     <  1:4183404/94‑1 (MQ=255)
                                     gcATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGAttt                                                     <  1:10095426/94‑1 (MQ=255)
                                      cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTg                                                    >  1:5890158/1‑94 (MQ=255)
                                      cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTg                                                    >  2:2565214/1‑94 (MQ=255)
                                      cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTg                                                    <  1:372108/94‑1 (MQ=255)
                                      cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTg                                                    <  2:7988271/94‑1 (MQ=255)
                                      cATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTg                                                    >  2:8860039/1‑94 (MQ=255)
                                       aTCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGc                                                   <  2:5561865/94‑1 (MQ=255)
                                       aTCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGc                                                   >  1:2216682/1‑94 (MQ=255)
                                       aTCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGc                                                   <  1:4909827/94‑1 (MQ=255)
                                       aTCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGc                                                   <  1:6211813/94‑1 (MQ=255)
                                         cGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCa                                                 >  1:8760408/1‑94 (MQ=255)
                                          ggCCAGTTTAGTTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCAt                                                <  2:5098150/94‑1 (MQ=255)
                                          ggCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCAt                                                >  1:8610229/1‑94 (MQ=255)
                                          ggCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCAt                                                <  1:8946256/94‑1 (MQ=255)
                                           gCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCAtt                                               <  2:1179227/94‑1 (MQ=255)
                                            ccAGTTTACGTTCCAGCCACTCAAGCTGACACTCGCTCAGCCACCCCGGCGGCGAGCCACACACCTGGCTATACAGCCACAGGATTGGCCATTg                                              >  2:5787436/1‑94 (MQ=255)
                                            ccAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTg                                              >  2:8986329/1‑94 (MQ=255)
                                             cAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGc                                             >  1:10234709/1‑94 (MQ=255)
                                             cAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGc                                             <  2:1493372/94‑1 (MQ=255)
                                             cAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGc                                             >  1:2912486/1‑94 (MQ=255)
                                             cAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGc                                             >  2:6455940/1‑94 (MQ=255)
                                             cAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGc                                             >  2:4177063/1‑94 (MQ=255)
                                              aGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCt                                            <  1:9584492/94‑1 (MQ=255)
                                              aGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCt                                            >  1:4062398/1‑94 (MQ=255)
                                              aGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCt                                            <  2:8407900/94‑1 (MQ=255)
                                              aGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCt                                            >  2:9292613/1‑94 (MQ=255)
                                               gTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTc                                           >  2:4042686/1‑94 (MQ=255)
                                               gTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTc                                           <  2:3083350/94‑1 (MQ=255)
                                                tttACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCa                                          <  2:8712223/94‑1 (MQ=255)
                                                  tACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCAcc                                        <  1:6707393/94‑1 (MQ=255)
                                                    cGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCaa                                      >  2:4680469/1‑94 (MQ=255)
                                                     ttcccAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAAt                                     <  1:3884814/91‑1 (MQ=255)
                                                     gTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAAt                                     >  2:6727596/1‑94 (MQ=255)
                                                     cttCCAGCCACTCAAACTGAATCTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAAt                                     <  1:2792852/93‑1 (MQ=255)
                                                      ttCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATa                                    >  1:4150215/1‑94 (MQ=255)
                                                        ccAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATaaa                                  >  1:4370172/1‑94 (MQ=255)
                                                          aGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAaca                                >  2:7178797/1‑94 (MQ=255)
                                                          aGCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAaca                                <  1:6595481/94‑1 (MQ=255)
                                                           gCCACTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAacac                               <  1:7691761/94‑1 (MQ=255)
                                                              aCTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACAcgcg                            >  1:3066288/1‑94 (MQ=255)
                                                              aCTCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACAcgcg                            >  2:2279362/1‑94 (MQ=255)
                                                                tCAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCt                          >  1:8458610/1‑94 (MQ=255)
                                                                 cAAGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCtt                         >  2:8224900/1‑94 (MQ=255)
                                                                   aGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCg                       <  1:9802008/94‑1 (MQ=255)
                                                                   aGCTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCg                       >  1:6024998/1‑94 (MQ=255)
                                                                     cTGAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGcc                     >  2:4904174/1‑94 (MQ=255)
                                                                       gAAACTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCgg                   >  1:6451965/1‑94 (MQ=255)
                                                                           cTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGgag               <  2:982760/94‑1 (MQ=255)
                                                                           cTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGgag               <  2:5485494/94‑1 (MQ=255)
                                                                           cTCGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGgag               <  1:5080079/94‑1 (MQ=255)
                                                                             cGCTCAGCTCACCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGAt             >  1:1905990/1‑94 (MQ=255)
                                                                              gctcagctcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATa            <  2:7408805/94‑1 (MQ=255)
                                                                                tcagctcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAcc          <  1:1826208/94‑1 (MQ=255)
                                                                                tcagctcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAcc          >  1:1086132/1‑94 (MQ=255)
                                                                                 cagctcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAccc         <  1:693053/94‑1 (MQ=255)
                                                                                 cagctcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATAccc         <  2:1649739/94‑1 (MQ=255)
                                                                                  agctcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCg        >  2:6840526/1‑94 (MQ=255)
                                                                                   gctcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGc       <  2:2905712/94‑1 (MQ=255)
                                                                                    ctcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCa      >  2:2010308/1‑94 (MQ=255)
                                                                                     tcaCCGCGCGGCCCGCCAAACACCTGGCTATCCAACAACAGGATTTGCCATTGCTCACCAATACACACGCGCTTCGCCGGGGAGATACCCGCAt     >  1:10000088/1‑94 (MQ=255)
                                                                                     tcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCAt     <  1:9181715/94‑1 (MQ=255)
                                                                                     tcaCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCAt     <  1:2864334/94‑1 (MQ=255)
                                                                                      caCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATc    >  1:3629279/1‑94 (MQ=255)
                                                                                       aCCGCGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATcc   >  1:808700/1‑94 (MQ=255)
                                                                                           |                                                                                          
ATGATGATGCAGCAGCAGCAACGTATGGCGTTCTGGCGCATCGGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCT  >  NC_000913/3174352‑3174533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 286 total aligned reads displayed.
AGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG  >  NC_000913/3175652‑3175838
                                                                                             |                                                                                             
AGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGG                                                                                               >  SRR4302545.5844008/1‑94 (MQ=60)
AGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGG                                                                                               >  SRR4302545.3459252/1‑94 (MQ=60)
AGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGG                                                                                               >  SRR4302545.8605804/1‑94 (MQ=60)
AGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGG                                                                                               >  SRR4302545.2032697/1‑94 (MQ=60)
AGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGG                                                                                               <  SRR4302545.4858243/94‑1 (MQ=60)
 GTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGC                                                                                              <  SRR4302545.2959061/94‑1 (MQ=60)
 GTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGC                                                                                              <  SRR4302545.2959059/94‑1 (MQ=60)
 GTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGC                                                                                              >  SRR4302545.9614806/1‑94 (MQ=60)
  TAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCT                                                                                             <  SRR4302545.7690030/94‑1 (MQ=60)
   AGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTG                                                                                            >  SRR4302545.8566499/1‑94 (MQ=60)
    GCTAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGC                                                                                           <  SRR4302545.5020072/94‑1 (MQ=60)
    GCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGC                                                                                           >  SRR4302545.1838964/1‑94 (MQ=60)
    GCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGC                                                                                           <  SRR4302545.10539549/94‑1 (MQ=60)
     CCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCG                                                                                          <  SRR4302545.9515051/94‑1 (MQ=60)
     CCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCG                                                                                          <  SRR4302545.1576909/94‑1 (MQ=60)
     CCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCG                                                                                          >  SRR4302545.6647284/1‑94 (MQ=60)
      CAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGG                                                                                         >  SRR4302545.4719124/1‑94 (MQ=60)
      CAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGG                                                                                         >  SRR4302545.3211720/1‑94 (MQ=60)
      CAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGG                                                                                         >  SRR4302545.9913011/1‑94 (MQ=60)
       AAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGC                                                                                        <  SRR4302545.2279190/94‑1 (MQ=60)
       AAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGC                                                                                        >  SRR4302545.3391772/1‑94 (MQ=60)
       AAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGC                                                                                        >  SRR4302545.1970492/1‑94 (MQ=60)
       AAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGC                                                                                        <  SRR4302545.2843162/94‑1 (MQ=60)
       AAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGC                                                                                        >  SRR4302545.159489/1‑94 (MQ=60)
       AAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGC                                                                                        <  SRR4302545.6578644/94‑1 (MQ=60)
       AAGGGATTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGC                                                                                        <  SRR4302545.7087653/94‑1 (MQ=60)
        AGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCG                                                                                       >  SRR4302545.8402398/1‑94 (MQ=60)
         GGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGT                                                                                      <  SRR4302545.3628173/94‑1 (MQ=60)
         GGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGT                                                                                      >  SRR4302545.7384225/1‑94 (MQ=60)
          GGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTG                                                                                     >  SRR4302545.8264324/1‑94 (MQ=60)
          GGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTG                                                                                     >  SRR4302545.3945042/1‑94 (MQ=60)
          GGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTG                                                                                     <  SRR4302545.2413656/94‑1 (MQ=60)
           GGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGA                                                                                    >  SRR4302545.8834767/1‑94 (MQ=60)
             TTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACC                                                                                  <  SRR4302545.2885399/94‑1 (MQ=60)
             TTTCGCTGGTGGCGTACGCGGCAATCCGAATCTACTCAAGCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACC                                                                                  <  SRR4302545.9823899/94‑1 (MQ=60)
               TCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCGATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGC                                                                                <  SRR4302545.4855271/94‑1 (MQ=60)
               TCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGC                                                                                >  SRR4302545.3043966/1‑94 (MQ=60)
                CGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCG                                                                               <  SRR4302545.8489135/94‑1 (MQ=60)
                CGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCG                                                                               <  SRR4302545.8683308/94‑1 (MQ=60)
                 GCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGC                                                                              >  SRR4302545.10176531/1‑94 (MQ=60)
                 GCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGC                                                                              >  SRR4302545.8991320/1‑94 (MQ=60)
                 GCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGC                                                                              >  SRR4302545.4700688/1‑94 (MQ=60)
                    GGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCA                                                                           >  SRR4302545.985099/1‑94 (MQ=60)
                    GGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCA                                                                           <  SRR4302545.10932889/94‑1 (MQ=60)
                      TGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAA                                                                         >  SRR4302545.2458424/1‑94 (MQ=60)
                       GTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAA                                                                        >  SRR4302545.9129094/1‑94 (MQ=60)
                         CGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAAT                                                                      <  SRR4302545.4677555/94‑1 (MQ=60)
                         CGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAAT                                                                      <  SRR4302545.9820907/94‑1 (MQ=60)
                          GTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATT                                                                     >  SRR4302545.7480006/1‑94 (MQ=60)
                           TACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTT                                                                    <  SRR4302545.3542818/94‑1 (MQ=60)
                             CGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCC                                                                  >  SRR4302545.7755333/1‑94 (MQ=60)
                             CGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCC                                                                  >  SRR4302545.6354743/1‑94 (MQ=60)
                               TGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCG                                                                >  SRR4302545.8010615/1‑94 (MQ=60)
                               CGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAACTTTCACG                                                                >  SRR4302545.6809540/1‑94 (MQ=60)
                               CGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCG                                                                <  SRR4302545.104364/94‑1 (MQ=60)
                               CGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCG                                                                <  SRR4302545.6817576/94‑1 (MQ=60)
                               CGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCG                                                                <  SRR4302545.4838268/94‑1 (MQ=60)
                                GGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGC                                                               <  SRR4302545.1241257/94‑1 (MQ=60)
                                GGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGC                                                               <  SRR4302545.5472229/94‑1 (MQ=60)
                                 GCAATCCGAATCTGCTCAATCAGCACACCTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCC                                                              <  SRR4302545.7400100/94‑1 (MQ=60)
                                 GCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCC                                                              <  SRR4302545.3968494/94‑1 (MQ=60)
                                 GCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCC                                                              <  SRR4302545.3838197/94‑1 (MQ=60)
                                 GCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCC                                                              <  SRR4302545.5890403/94‑1 (MQ=60)
                                 GCAATCCGAAGCAGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCC                                                              <  SRR4302545.7400101/94‑1 (MQ=60)
                                   AATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGC                                                            >  SRR4302545.7617813/1‑94 (MQ=60)
                                   AATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGC                                                            >  SRR4302545.9317981/1‑94 (MQ=60)
                                   AATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGC                                                            >  SRR4302545.8010066/1‑94 (MQ=60)
                                   AATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGAAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGC                                                            >  SRR4302545.9317982/1‑94 (MQ=60)
                                    ATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCA                                                           >  SRR4302545.1468255/1‑94 (MQ=60)
                                     TCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCAC                                                          >  SRR4302545.7821938/1‑94 (MQ=60)
                                      CCGAATCTGCTCAATCCGCACCACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACC                                                         >  SRR4302545.1276069/1‑94 (MQ=60)
                                      CCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACC                                                         >  SRR4302545.2432730/1‑94 (MQ=60)
                                      CCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACC                                                         >  SRR4302545.7511882/1‑94 (MQ=60)
                                       CGAATCTTCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGTTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCT                                                        <  SRR4302545.5738902/94‑1 (MQ=60)
                                       CGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCT                                                        >  SRR4302545.9851674/1‑94 (MQ=60)
                                       CGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCT                                                        >  SRR4302545.3211743/1‑94 (MQ=60)
                                        GAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTC                                                       <  SRR4302545.8373671/94‑1 (MQ=60)
                                        GAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTC                                                       >  SRR4302545.8095057/1‑94 (MQ=60)
                                        GAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTC                                                       >  SRR4302545.1550093/1‑94 (MQ=60)
                                         AATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCA                                                      >  SRR4302545.720310/1‑94 (MQ=60)
                                         AATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCA                                                      >  SRR4302545.4041617/1‑94 (MQ=60)
                                         AATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCA                                                      >  SRR4302545.41460/1‑94 (MQ=60)
                                          ATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAT                                                     <  SRR4302545.10515769/94‑1 (MQ=60)
                                          ATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAT                                                     <  SRR4302545.8436138/94‑1 (MQ=60)
                                          ATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAT                                                     <  SRR4302545.1890316/94‑1 (MQ=60)
                                          ATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAT                                                     <  SRR4302545.4509479/94‑1 (MQ=60)
                                          ATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCAT                                                     >  SRR4302545.9166056/1‑94 (MQ=60)
                                           TCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATG                                                    <  SRR4302545.690057/94‑1 (MQ=60)
                                            CTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGA                                                   >  SRR4302545.6793993/1‑94 (MQ=60)
                                             TGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAC                                                  >  SRR4302545.1115812/1‑94 (MQ=60)
                                             TGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAC                                                  >  SRR4302545.1115811/1‑94 (MQ=60)
                                             TGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAC                                                  <  SRR4302545.9881431/94‑1 (MQ=60)
                                             TGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAC                                                  <  SRR4302545.1744913/94‑1 (MQ=60)
                                              GCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACT                                                 >  SRR4302545.10717818/1‑94 (MQ=60)
                                               CTCAATCAGCCCAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTC                                                <  SRR4302545.913881/94‑1 (MQ=60)
                                               CTCAATCAGCACAACTTCATCGCGCACTGGGTCAAAGGGTAGTAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTC                                                >  SRR4302545.6968089/1‑94 (MQ=60)
                                               CTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTC                                                <  SRR4302545.8212914/94‑1 (MQ=60)
                                                TCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCA                                               <  SRR4302545.7008114/94‑1 (MQ=60)
                                                  AATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATT                                             >  SRR4302545.6873052/1‑94 (MQ=60)
                                                   ATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTT                                            <  SRR4302545.6536444/94‑1 (MQ=60)
                                                    TCGGCACAACTTCATCACGCACTCAGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTG                                           <  SRR4302545.6025186/94‑1 (MQ=60)
                                                    TCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTG                                           <  SRR4302545.10851258/94‑1 (MQ=60)
                                                    TCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTG                                           <  SRR4302545.6201916/94‑1 (MQ=60)
                                                    TCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTG                                           <  SRR4302545.909360/94‑1 (MQ=60)
                                                      AGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCC                                         <  SRR4302545.4567022/94‑1 (MQ=60)
                                                      AGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCC                                         >  SRR4302545.6382486/1‑94 (MQ=60)
                                                        CACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCG                                       <  SRR4302545.9167090/94‑1 (MQ=60)
                                                        CACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCG                                       >  SRR4302545.1792811/1‑94 (MQ=60)
                                                        CACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCACG                                       >  SRR4302545.3287270/1‑94 (MQ=60)
                                                        CACAACTCCATCGCGCACGGGGTCAAGGGGTAGCAAGGCTGCGGCGTGCCCGCGCAAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCG                                       <  SRR4302545.9563252/94‑1 (MQ=53)
                                                         CCAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGT                                      <  SRR4302545.3075481/94‑1 (MQ=60)
                                                         ACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCTCACCTCATGACTCATTTGCCCGT                                      >  SRR4302545.5961469/1‑94 (MQ=60)
                                                         ACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGT                                      >  SRR4302545.9385172/1‑94 (MQ=60)
                                                         ACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGT                                      >  SRR4302545.5961468/1‑94 (MQ=60)
                                                         ACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGAGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGT                                      <  SRR4302545.10262356/94‑1 (MQ=60)
                                                          CAACTTCATCCCGCCCTGGGTCAAAGGGTAGCAAGGCTGCGGCGGGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTT                                     <  SRR4302545.660732/94‑1 (MQ=60)
                                                          CAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTT                                     >  SRR4302545.504744/1‑94 (MQ=60)
                                                          CAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTT                                     <  SRR4302545.8520005/94‑1 (MQ=60)
                                                          CAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTT                                     >  SRR4302545.10160101/1‑94 (MQ=60)
                                                          CAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTT                                     >  SRR4302545.2973128/1‑94 (MQ=60)
                                                          CAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTT                                     >  SRR4302545.130712/1‑94 (MQ=60)
                                                          CAACTCCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTT                                     <  SRR4302545.6186947/94‑1 (MQ=60)
                                                           AACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTG                                    <  SRR4302545.2835820/94‑1 (MQ=60)
                                                           AACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAACAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTG                                    >  SRR4302545.4496170/1‑94 (MQ=60)
                                                           AACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTG                                    <  SRR4302545.9566552/94‑1 (MQ=60)
                                                            ACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGA                                   <  SRR4302545.7949892/94‑1 (MQ=60)
                                                            ACTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGA                                   <  SRR4302545.10385067/94‑1 (MQ=60)
                                                            ACTCCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGA                                   <  SRR4302545.4215246/94‑1 (MQ=60)
                                                             CTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAA                                  <  SRR4302545.2354946/94‑1 (MQ=60)
                                                             CTTCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAA                                  >  SRR4302545.6729195/1‑94 (MQ=60)
                                                               TCATCACGCACTGGGTCAAAGGGTAGCAGGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATA                                <  SRR4302545.3381579/94‑1 (MQ=60)
                                                               TCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGCCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATA                                <  SRR4302545.856698/94‑1 (MQ=60)
                                                               TCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATA                                >  SRR4302545.8814882/1‑94 (MQ=60)
                                                               TCATCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATA                                <  SRR4302545.4945366/94‑1 (MQ=60)
                                                               TCATCACGCACGGGGTCAAAGGGTAGCAAGCCTCCGGCGTGACCGCGCTCAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATA                                <  SRR4302545.6169782/94‑1 (MQ=60)
                                                                  TCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGAC                             <  SRR4302545.4296167/94‑1 (MQ=60)
                                                                  TCACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGAC                             <  SRR4302545.3230531/94‑1 (MQ=60)
                                                                   CACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACG                            >  SRR4302545.3994388/1‑94 (MQ=60)
                                                                   CACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACG                            <  SRR4302545.6935026/94‑1 (MQ=60)
                                                                   CACGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACG                            >  SRR4302545.3368489/1‑94 (MQ=60)
                                                                    CCGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGA                           <  SRR4302545.7403403/94‑1 (MQ=60)
                                                                     CGCGCTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAT                          <  SRR4302545.3548323/94‑1 (MQ=60)
                                                                     CGCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAT                          <  SRR4302545.4828412/94‑1 (MQ=60)
                                                                     CGCACTGGGTCAAAGGGCAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGACCGTTGActagccgct                          >  SRR4302545.1045509/1‑85 (MQ=60)
                                                                      GCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATG                         <  SRR4302545.25060/94‑1 (MQ=60)
                                                                      GCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATG                         >  SRR4302545.7427457/1‑94 (MQ=60)
                                                                      GCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATG                         <  SRR4302545.829265/94‑1 (MQ=60)
                                                                      GCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATG                         >  SRR4302545.389324/1‑94 (MQ=60)
                                                                      GCACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATG                         <  SRR4302545.8102237/94‑1 (MQ=60)
                                                                       CACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCCCAAAAATTTCCGCCCGAACCCCATGACTAATTTCCCCGTTGAATAGACGATac                        >  SRR4302545.2288535/1‑92 (MQ=60)
                                                                        CCTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAC                       <  SRR4302545.8996329/94‑1 (MQ=60)
                                                                        CCTGGGTCAAAGGCTGGCAAGGCTGCGACGTGGCCGTGCTGAAGAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAC                       <  SRR4302545.5131359/94‑1 (MQ=60)
                                                                        ACTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAC                       >  SRR4302545.5306698/1‑94 (MQ=60)
                                                                        ACGGGGTAAAAGGGTAGCAAGCCGGCGGCGAGCCCGCTCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAC                       <  SRR4302545.358787/94‑1 (MQ=60)
                                                                         CTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACG                      >  SRR4302545.10014162/1‑94 (MQ=60)
                                                                         CTGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACG                      <  SRR4302545.140451/94‑1 (MQ=60)
                                                                          TGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGA                     <  SRR4302545.5733448/94‑1 (MQ=60)
                                                                          TGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGA                     >  SRR4302545.4923848/1‑94 (MQ=60)
                                                                          TGGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGA                     >  SRR4302545.4619874/1‑94 (MQ=60)
                                                                           GGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAA                    <  SRR4302545.206597/94‑1 (MQ=60)
                                                                           GGGTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAA                    <  SRR4302545.2702706/94‑1 (MQ=60)
                                                                             GTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAAT                  <  SRR4302545.10247035/94‑1 (MQ=60)
                                                                             GTCAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAAT                  <  SRR4302545.3734180/94‑1 (MQ=60)
                                                                              gtAAAGGGTAGCAAGGCTGCGGCGGGACCGCACTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATC                 <  SRR4302545.2941054/92‑1 (MQ=60)
                                                                               CAAAGGGTAGCAGGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCT                <  SRR4302545.7843577/94‑1 (MQ=60)
                                                                               CAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCT                <  SRR4302545.7843578/94‑1 (MQ=60)
                                                                               CAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCT                >  SRR4302545.9513124/1‑94 (MQ=60)
                                                                               CAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCT                >  SRR4302545.4386300/1‑94 (MQ=60)
                                                                               CAAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCT                >  SRR4302545.4591867/1‑94 (MQ=60)
                                                                                AAAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTA               >  SRR4302545.729582/1‑94 (MQ=60)
                                                                                 AAGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTAT              >  SRR4302545.3770419/1‑94 (MQ=60)
                                                                                  AGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATA             <  SRR4302545.7616806/94‑1 (MQ=60)
                                                                                  AGGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATA             <  SRR4302545.3078637/94‑1 (MQ=60)
                                                                                   GGGTAGCAAGGCTGCGGCGTGACCGCTCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAA            <  SRR4302545.3006649/94‑1 (MQ=60)
                                                                                   GGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAA            >  SRR4302545.4144645/1‑94 (MQ=60)
                                                                                   GGGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAA            <  SRR4302545.3006648/94‑1 (MQ=60)
                                                                                    GGTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAA           >  SRR4302545.2324825/1‑94 (MQ=60)
                                                                                     GTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAG          <  SRR4302545.1130164/94‑1 (MQ=60)
                                                                                     GTAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAG          <  SRR4302545.1682368/94‑1 (MQ=60)
                                                                                      TAGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGA         <  SRR4302545.10005854/94‑1 (MQ=60)
                                                                                       AGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAT        <  SRR4302545.3487054/94‑1 (MQ=60)
                                                                                       AGCAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAT        >  SRR4302545.2673393/1‑94 (MQ=60)
                                                                                         CAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCT      >  SRR4302545.6428149/1‑94 (MQ=60)
                                                                                         CAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCT      <  SRR4302545.10608648/94‑1 (MQ=60)
                                                                                         CAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCT      >  SRR4302545.6541852/1‑94 (MQ=60)
                                                                                         CAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCT      <  SRR4302545.2577281/94‑1 (MQ=60)
                                                                                         CAAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCT      <  SRR4302545.4370965/94‑1 (MQ=60)
                                                                                          AAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTA     >  SRR4302545.6245538/1‑94 (MQ=60)
                                                                                          AAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTA     >  SRR4302545.5245097/1‑94 (MQ=60)
                                                                                          AAGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTA     >  SRR4302545.4583784/1‑94 (MQ=60)
                                                                                           AGTCTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAA    <  SRR4302545.220064/94‑1 (MQ=60)
                                                                                           AGGCTGCGGCGTGACCGCGCTCATAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAA    <  SRR4302545.7798618/94‑1 (MQ=60)
                                                                                           AGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAA    >  SRR4302545.4912438/1‑94 (MQ=60)
                                                                                           AGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAA    <  SRR4302545.953894/94‑1 (MQ=60)
                                                                                           AGGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAA    >  SRR4302545.7425646/1‑94 (MQ=60)
                                                                                            GGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAT   >  SRR4302545.2021752/1‑94 (MQ=60)
                                                                                            GGCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAT   >  SRR4302545.519147/1‑94 (MQ=60)
                                                                                            GGCTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAAT   <  SRR4302545.7575571/94‑1 (MQ=60)
                                                                                             GCTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG  <  SRR4302545.9249888/94‑1 (MQ=60)
                                                                                             GCTGCGGCGTGACCGCGCTCAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG  <  SRR4302545.4195618/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG  >  NC_000913/3175652‑3175838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑