Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,268,129 (C)7→8 intergenic (+661/+137) b3122 → / ← b3124 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,268,1221.C94.5% 901.2 / 37.6 273intergenic (+654/+144)b3122/b3124hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (9/6);  new base C (151/107);  total (160/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 347 total aligned reads displayed.

CGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAA  >  NC_000913/3268029‑3268210
                                                                                               |                                                                                         
cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGttt                                                                                            <  2:231820/94‑1 (MQ=255)
cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGGCGT‑GGGttt                                                                                            >  1:3386732/1‑94 (MQ=255)
cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTAACTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGGCGT‑GGGttt                                                                                            >  2:750229/1‑94 (MQ=255)
  tggTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTTGTGCACTGGGCGT‑GGGTTT‑‑cc                                                                                        >  1:1134171/2‑94 (MQ=255)
  cgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑cc                                                                                        <  1:5657005/94‑1 (MQ=255)
    gTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑cccc                                                                                      <  2:166124/94‑1 (MQ=255)
     tGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑ccccc                                                                                     >  2:8768334/1‑94 (MQ=255)
       ggtgctTACCGAACCATTTCCCCTTTACCTGATCCCGCTTGGGCGGGCGATCGGCGGTTTGCGCTCTGTGGCACTGGTCGT‑GGGTTT‑‑ccccccc                                                                                   <  2:6437398/90‑1 (MQ=255)
       cgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑ccccccc                                                                                   <  2:130204/94‑1 (MQ=255)
       cgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑ccccccc                                                                                   >  1:3780974/1‑94 (MQ=255)
        gcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑cccccccc                                                                                  >  1:7641364/1‑93 (MQ=255)
        gcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑cccccccc                                                                                  >  1:6109041/1‑93 (MQ=255)
        gcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑cccccccc                                                                                  <  2:2555047/94‑2 (MQ=255)
         ctctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑cccccccca                                                                                 <  1:9734632/94‑3 (MQ=255)
         ctctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑cccccccca                                                                                 <  2:4093539/94‑3 (MQ=255)
          tctTACCGCCCCCTCTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑ccccccccag                                                                                <  1:8619262/94‑4 (MQ=255)
          tctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑ccccccccag                                                                                >  1:1228507/1‑91 (MQ=255)
           ctTACCGCCCCCTTTCCCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑cccccccccag                                                                               <  1:3515948/94‑5 (MQ=255)
           ctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑ccccccccagg                                                                               <  2:3662244/94‑5 (MQ=255)
             tACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCg                                                                              <  2:7419579/94‑1 (MQ=255)
               ccGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCggt                                                                            >  1:2313288/1‑92 (MQ=255)
               ccGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGtt                                                                            >  2:5907193/1‑94 (MQ=255)
                cGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTa                                                                           >  2:3222471/1‑94 (MQ=255)
                cGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTa                                                                           <  2:8580720/94‑1 (MQ=255)
                cGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGCTa                                                                           >  1:9319428/1‑94 (MQ=255)
                  cACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTAcc                                                                         >  1:5100348/1‑94 (MQ=255)
                   ccccTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCt                                                                        <  1:598562/93‑1 (MQ=255)
                   aCCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGGTTC‑‑CCCCCCCAGGCGTTACCt                                                                        >  2:6665915/1‑94 (MQ=255)
                   aCCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCt                                                                        >  1:5260729/1‑94 (MQ=255)
                     ccTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCCCAGGCGTTACCTg                                                                       >  2:8303931/1‑94 (MQ=255)
                     ccTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTgg                                                                      >  2:10469518/1‑94 (MQ=255)
                     ccTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTgg                                                                      <  1:2413857/94‑1 (MQ=255)
                      cTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGc                                                                     <  2:1901330/94‑1 (MQ=255)
                       tttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTGCTCTCTGTTGCACTGGTCGTGGGGTTC‑‑CCCCCCCAGGCGTTACCTGGCa                                                                    >  2:9739642/1‑94 (MQ=255)
                        ttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCAc                                                                   >  2:10013199/1‑94 (MQ=255)
                        ttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑CCCCCCCAGGCGTTACCTGGCAcc                                                                  <  1:7235607/94‑1 (MQ=255)
                           ccccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCt                                                                <  1:54603/93‑1 (MQ=255)
                           aCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCt                                                                <  2:9198352/94‑1 (MQ=255)
                            cccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTg                                                               >  1:3052859/1‑94 (MQ=255)
                            cccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTg                                                               <  2:2067842/94‑1 (MQ=255)
                            cccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTg                                                               >  2:6281408/1‑94 (MQ=255)
                            cccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTg                                                               >  2:6317470/1‑94 (MQ=255)
                             ccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGc                                                              >  2:8348011/1‑94 (MQ=255)
                              cTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGcc                                                             <  2:10064030/94‑1 (MQ=255)
                                  ccTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAt                                                         >  1:5376565/1‑94 (MQ=255)
                                  ccTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAt                                                         <  2:2221812/94‑1 (MQ=255)
                                  ccTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTAt                                                         >  1:6180949/1‑94 (MQ=255)
                                     gATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATggg                                                      >  2:2811931/1‑93 (MQ=255)
                                      aTCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAg                                                     <  1:890116/94‑1 (MQ=255)
                                      aTCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAg                                                     <  1:3417226/94‑1 (MQ=255)
                                      aTCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAg                                                     >  1:5602810/1‑94 (MQ=255)
                                       tCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGcc                                                   <  1:4373941/94‑1 (MQ=255)
                                        cccGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGcc                                                   >  2:7746690/1‑94 (MQ=255)
                                         ccGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGccc                                                  >  2:1151095/1‑94 (MQ=255)
                                          cGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCg                                                 <  1:7461193/94‑1 (MQ=255)
                                           gCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCgg                                                >  2:8924417/1‑94 (MQ=255)
                                            cTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGa                                               >  2:10303603/1‑94 (MQ=255)
                                             ttGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGAc                                              <  2:7901559/94‑1 (MQ=255)
                                                cgcgGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACttt                                           >  2:4643586/1‑94 (MQ=255)
                                                  cgGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcc                                         <  2:6003083/94‑1 (MQ=255)
                                                   gggCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcct                                        >  1:9596673/1‑94 (MQ=255)
                                                   gggCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcct                                        <  2:4185322/94‑1 (MQ=255)
                                                    ggCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcctc                                       <  2:3018641/94‑1 (MQ=255)
                                                    ggCCATCGGCGGTGTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcctc                                       <  1:1414726/94‑1 (MQ=255)
                                                      ccATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctccc                                     <  2:1019501/94‑1 (MQ=255)
                                                       cATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccc                                    <  1:8433071/94‑1 (MQ=255)
                                                       cATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccct                                   <  1:8747650/94‑1 (MQ=255)
                                                        aTCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGGTTC‑‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctccccc                                   >  2:952954/1‑93 (MQ=255)
                                                        aTCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccct                                   <  1:8081495/94‑1 (MQ=255)
                                                        aTCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccct                                   >  1:6325980/1‑94 (MQ=255)
                                                          cggcggTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccctcc                                 <  2:3525097/94‑1 (MQ=255)
                                                           ggcggTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGGCCGGACTTTCCTCCCCTCCg                                >  1:1312957/1‑94 (MQ=255)
                                                           ggcggTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCg                                >  2:3918279/1‑94 (MQ=255)
                                                           ggcggTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTACCCTATGGAGCCCGGACTTTCCTCCCCTCCg                                >  2:5181113/1‑94 (MQ=255)
                                                             cggTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGcc                              <  2:6180949/94‑1 (MQ=255)
                                                              ggTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGccc                             <  2:8430334/94‑1 (MQ=255)
                                                               gTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCg                            >  1:2722845/1‑94 (MQ=255)
                                                                   gCTCTCTGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGtctc                        >  1:7078622/1‑94 (MQ=255)
                                                                    ctctctGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTcc                       >  2:728146/1‑94 (MQ=255)
                                                                      ctctGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTcccc                     >  2:4330467/1‑94 (MQ=255)
                                                                      ctctGTTGCACTGGTCGT‑GGGTTT‑‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTccccc                    >  2:6987586/1‑94 (MQ=255)
                                                                        ctGTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCg                   <  1:9846321/94‑1 (MQ=255)
                                                                          gTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGaa                 >  1:1040462/1‑94 (MQ=255)
                                                                          gTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGaa                 >  2:5567832/1‑94 (MQ=255)
                                                                          gTTGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGaa                 >  1:9134521/1‑94 (MQ=255)
                                                                           ttGCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAag                <  2:2493239/94‑1 (MQ=255)
                                                                            tGCCCTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTCCCTCCCCTCCGCCCGTCTCCCCCGAaga               <  2:1472622/94‑1 (MQ=255)
                                                                             gCACTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAagag              >  2:7585309/1‑94 (MQ=255)
                                                                               aCTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGga            >  1:148216/1‑94 (MQ=255)
                                                                               aCTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGga            >  2:8733204/1‑94 (MQ=255)
                                                                                cTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGCGgac           >  1:2049529/1‑94 (MQ=255)
                                                                                cTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAggcc           >  2:2890513/1‑92 (MQ=255)
                                                                                cTGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgac           <  1:2202750/94‑1 (MQ=255)
                                                                                 tGGTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacg          <  1:309268/94‑1 (MQ=255)
                                                                                  ggTCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacga         <  2:6854707/94‑1 (MQ=255)
                                                                                    tCGT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacgacg       <  1:6924967/94‑1 (MQ=255)
                                                                                      gT‑GGTTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCCCCCCTCCGCCCGTCTCCCCCGAAGAGgacgacgac     <  1:10052651/94‑1 (MQ=255)
                                                                                      gT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacgacgac     <  1:1712392/94‑1 (MQ=255)
                                                                                      gT‑GGGTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacgacgac     >  1:4989462/1‑94 (MQ=255)
                                                                                         gggTTTC‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGAcgacgacga   <  1:3847438/94‑1 (MQ=255)
                                                                                               |                                                                                         
CGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTT‑‑CCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAA  >  NC_000913/3268029‑3268210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 273 total aligned reads displayed.
GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279105‑3279290
                                                                                            |                                                                                             
GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCG                                                                                              >  SRR4302545.8786639/1‑94 (MQ=60)
 CGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGT                                                                                             <  SRR4302545.2748846/94‑1 (MQ=60)
 CGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGGGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGT                                                                                             >  SRR4302545.9359403/1‑94 (MQ=60)
  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            <  SRR4302545.2313208/94‑1 (MQ=60)
  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302545.546039/1‑94 (MQ=60)
  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302545.4107507/1‑94 (MQ=60)
   GAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCG                                                                                           >  SRR4302545.5912882/1‑94 (MQ=60)
   GAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCG                                                                                           >  SRR4302545.4313483/1‑94 (MQ=60)
    AAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGT                                                                                          <  SRR4302545.6970789/94‑1 (MQ=60)
    AAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGT                                                                                          >  SRR4302545.2235427/1‑94 (MQ=60)
     AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         <  SRR4302545.573036/94‑1 (MQ=60)
     AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         >  SRR4302545.1859408/1‑94 (MQ=60)
      accacaCATATGGCCTCGTTGCATCCGGCGCGAATGCTGGGTGTTGAGGGTTTTTTGGTATCGCTTAACCCGGCCAAGCGCGCCAGTGTCGTTG                                                                                        <  SRR4302545.9522544/88‑1 (MQ=32)
      GCCGTCCCTATGGCGTCGCTGCCTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCCGCGTCGTTG                                                                                        <  SRR4302545.8309037/94‑1 (MQ=60)
      GCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        >  SRR4302545.2985075/1‑94 (MQ=60)
      GCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        <  SRR4302545.8567130/94‑1 (MQ=60)
      ACCATCCATATGGCGTCGCTGCCTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        <  SRR4302545.8567131/94‑1 (MQ=60)
       CCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGC                                                                                       >  SRR4302545.4470015/1‑94 (MQ=60)
       CCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGC                                                                                       >  SRR4302545.2049666/1‑94 (MQ=60)
       CCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGC                                                                                       >  SRR4302545.8907399/1‑94 (MQ=60)
       CCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCCGGCAAACGCGCCAGCGTCGTTGC                                                                                       >  SRR4302545.10331539/1‑94 (MQ=60)
         ATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGC                                                                                     >  SRR4302545.5906260/1‑94 (MQ=60)
         ATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGC                                                                                     >  SRR4302545.9561952/1‑94 (MQ=60)
          TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    >  SRR4302545.3359751/1‑94 (MQ=60)
          TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    <  SRR4302545.6201609/94‑1 (MQ=60)
          TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    <  SRR4302545.373234/94‑1 (MQ=60)
           gctTATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   <  SRR4302545.8864694/91‑1 (MQ=60)
           CCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGGTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   <  SRR4302545.8768071/94‑1 (MQ=60)
           CCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   <  SRR4302545.7696263/94‑1 (MQ=60)
           CCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   <  SRR4302545.7075906/94‑1 (MQ=60)
           CCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   >  SRR4302545.2431336/1‑94 (MQ=60)
           CCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   <  SRR4302545.1874645/94‑1 (MQ=60)
           CCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   >  SRR4302545.1744432/1‑94 (MQ=60)
            CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  <  SRR4302545.1940048/94‑1 (MQ=60)
             ATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGA                                                                                 >  SRR4302545.4175154/1‑94 (MQ=60)
              TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                >  SRR4302545.8877065/1‑94 (MQ=60)
              TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                >  SRR4302545.3803975/1‑94 (MQ=60)
               ATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATA                                                                               <  SRR4302545.8701675/94‑1 (MQ=60)
               ATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATA                                                                               <  SRR4302545.6180333/94‑1 (MQ=60)
               ATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTAGATA                                                                               <  SRR4302545.2348612/94‑1 (MQ=60)
                TGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302545.10152759/94‑1 (MQ=60)
                TGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302545.50776/94‑1 (MQ=60)
                 GGCGTCGCTTCAGCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             <  SRR4302545.2131180/94‑1 (MQ=60)
                 GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             <  SRR4302545.5574963/94‑1 (MQ=60)
                 GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             <  SRR4302545.174254/94‑1 (MQ=60)
                  GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302545.3643550/94‑1 (MQ=60)
                  GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302545.1424981/94‑1 (MQ=60)
                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302545.3743453/94‑1 (MQ=60)
                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302545.5482088/94‑1 (MQ=60)
                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302545.1117580/94‑1 (MQ=60)
                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302545.1605004/94‑1 (MQ=60)
                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          <  SRR4302545.9231822/94‑1 (MQ=60)
                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          <  SRR4302545.8696429/94‑1 (MQ=60)
                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          >  SRR4302545.10259474/1‑94 (MQ=60)
                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          >  SRR4302545.9251227/1‑94 (MQ=60)
                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          >  SRR4302545.2073563/1‑94 (MQ=60)
                      CGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCT                                                                        >  SRR4302545.3858678/1‑94 (MQ=60)
                      CGCGGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCT                                                                        <  SRR4302545.3578925/94‑1 (MQ=60)
                       GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       <  SRR4302545.4306436/94‑1 (MQ=60)
                         TGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACA                                                                     >  SRR4302545.6519202/1‑94 (MQ=60)
                          GCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    <  SRR4302545.5367470/94‑1 (MQ=60)
                          GCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    >  SRR4302545.8369772/1‑94 (MQ=60)
                           CATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATG                                                                   >  SRR4302545.9953189/1‑94 (MQ=60)
                           CATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATG                                                                   <  SRR4302545.10233734/94‑1 (MQ=60)
                            ATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  >  SRR4302545.6900663/1‑94 (MQ=60)
                            ATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  <  SRR4302545.3254932/94‑1 (MQ=60)
                            ATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGG                                                                  >  SRR4302545.868055/1‑94 (MQ=60)
                               CGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCA                                                               >  SRR4302545.10853632/1‑94 (MQ=60)
                               CGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCA                                                               <  SRR4302545.8188292/94‑1 (MQ=60)
                                GGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAA                                                              <  SRR4302545.9048956/94‑1 (MQ=60)
                                GGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAA                                                              <  SRR4302545.3835130/94‑1 (MQ=60)
                                 GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             >  SRR4302545.4888431/1‑94 (MQ=60)
                                 GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302545.6497222/94‑1 (MQ=60)
                                 GCGCGAATGCTGGGTGTTGATGGTGTTCGGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302545.4088416/94‑1 (MQ=60)
                                  CGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACA                                                            >  SRR4302545.4049537/1‑94 (MQ=60)
                                  CGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACA                                                            <  SRR4302545.4532471/94‑1 (MQ=60)
                                   GCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAA                                                           >  SRR4302545.2686156/1‑94 (MQ=60)
                                    CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          >  SRR4302545.3294404/1‑94 (MQ=60)
                                    CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          <  SRR4302545.5937270/94‑1 (MQ=60)
                                     GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         <  SRR4302545.10759729/94‑1 (MQ=60)
                                     GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         <  SRR4302545.5584393/94‑1 (MQ=60)
                                      AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        >  SRR4302545.6686862/1‑94 (MQ=60)
                                      AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        >  SRR4302545.1402519/1‑94 (MQ=60)
                                      AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        >  SRR4302545.9478200/1‑94 (MQ=60)
                                       ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       >  SRR4302545.3111093/1‑94 (MQ=60)
                                        TGCTGGGTGTTGATTGTGTTGTGGGATCGCTTAAACCGGGCAAACGCGCCAGGGTCGGTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      <  SRR4302545.8167762/94‑1 (MQ=60)
                                        TGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      >  SRR4302545.437557/1‑94 (MQ=60)
                                        TGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      <  SRR4302545.1323111/94‑1 (MQ=60)
                                         GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     >  SRR4302545.6804955/1‑94 (MQ=60)
                                         GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     <  SRR4302545.9848114/94‑1 (MQ=60)
                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    >  SRR4302545.7130189/1‑94 (MQ=60)
                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    >  SRR4302545.3452207/1‑94 (MQ=60)
                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    >  SRR4302545.5758278/1‑94 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   <  SRR4302545.7174711/94‑1 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   >  SRR4302545.10300035/1‑94 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   >  SRR4302545.7503930/1‑94 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   >  SRR4302545.10045978/1‑94 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   >  SRR4302545.5882744/1‑94 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   >  SRR4302545.9451180/1‑94 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   <  SRR4302545.2031924/94‑1 (MQ=60)
                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   >  SRR4302545.3661196/1‑94 (MQ=60)
                                            GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  >  SRR4302545.6098539/1‑94 (MQ=60)
                                            GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  <  SRR4302545.10657985/94‑1 (MQ=60)
                                            GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  >  SRR4302545.3342173/1‑94 (MQ=60)
                                             GGTGTTGATGGGGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCCACAAATCTGGATTC                                                 >  SRR4302545.9147031/1‑94 (MQ=60)
                                              GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                >  SRR4302545.968825/1‑94 (MQ=60)
                                              GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                <  SRR4302545.8387518/94‑1 (MQ=60)
                                               TGTTGATTGTGTTCTGTGATCGCTTAAACCGGGCAAACGCGTCAGCCTCGTGGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               <  SRR4302545.10027262/94‑1 (MQ=60)
                                               TGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               >  SRR4302545.7665867/1‑94 (MQ=60)
                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              >  SRR4302545.5590891/1‑94 (MQ=60)
                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302545.5126965/94‑1 (MQ=60)
                                                 TTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             <  SRR4302545.5490766/94‑1 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGCTTCAGGGT                                            >  SRR4302545.3012539/1‑94 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            <  SRR4302545.3547164/94‑1 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            <  SRR4302545.368859/94‑1 (MQ=60)
                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGC                                            >  SRR4302545.3012538/1‑94 (MQ=60)
                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           >  SRR4302545.6829543/1‑94 (MQ=60)
                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           <  SRR4302545.6518601/94‑1 (MQ=60)
                                                      GGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAT                                        >  SRR4302545.8328161/1‑94 (MQ=60)
                                                       GTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATT                                       <  SRR4302545.2658637/94‑1 (MQ=60)
                                                       GTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATT                                       <  SRR4302545.7341537/94‑1 (MQ=60)
                                                        TGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTA                                      >  SRR4302545.3103575/1‑94 (MQ=60)
                                                         GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACGAATCTGGATTCAGGGTCAATTAG                                     >  SRR4302545.6032882/1‑94 (MQ=60)
                                                         GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCTATTAG                                     >  SRR4302545.6410211/1‑94 (MQ=60)
                                                         GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     <  SRR4302545.943164/94‑1 (MQ=60)
                                                         GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     >  SRR4302545.8423811/1‑94 (MQ=60)
                                                         GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     >  SRR4302545.8620941/1‑94 (MQ=60)
                                                         GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     >  SRR4302545.2025004/1‑94 (MQ=60)
                                                          TTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGC                                    >  SRR4302545.7980826/1‑94 (MQ=60)
                                                           TCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCT                                   <  SRR4302545.1212775/94‑1 (MQ=60)
                                                            CTGGGATCGCTTAAACCGGGCAAGCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  <  SRR4302545.7806237/94‑1 (MQ=60)
                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  >  SRR4302545.7579241/1‑94 (MQ=60)
                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  >  SRR4302545.10510373/1‑94 (MQ=60)
                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  >  SRR4302545.430511/1‑94 (MQ=60)
                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  >  SRR4302545.6430248/1‑94 (MQ=60)
                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  <  SRR4302545.9124663/94‑1 (MQ=60)
                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 >  SRR4302545.8638334/1‑94 (MQ=60)
                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 <  SRR4302545.5092984/94‑1 (MQ=60)
                                                              GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                <  SRR4302545.10107969/94‑1 (MQ=60)
                                                               GGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGT                               <  SRR4302545.10133522/94‑1 (MQ=60)
                                                                gatcaattaagcaggaaAAACGCGCCAGCGTCATTGAGCTGGGTAGCAAGCTACATGTGCAACAAATCTGAATTCCGGGTCAATTAGCTACGTT                              <  SRR4302545.9630475/77‑1 (MQ=23)
                                                                GATCGCTTAGACCGGGCAAGCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGGGCAACAAATCTGGATTCAGGGTCTATTAGCTTCGTT                              <  SRR4302545.4356510/94‑1 (MQ=60)
                                                                GATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTT                              >  SRR4302545.7887042/1‑94 (MQ=60)
                                                                GATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTT                              >  SRR4302545.7887041/1‑94 (MQ=60)
                                                                  gcccgctatCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATagatcagagatc                            >  SRR4302545.1115922/10‑82 (MQ=60)
                                                                  TCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            <  SRR4302545.3199590/94‑1 (MQ=60)
                                                                  TCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            <  SRR4302545.6424147/94‑1 (MQ=60)
                                                                   TGCTTAAACCTGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTG                           <  SRR4302545.4146046/94‑1 (MQ=60)
                                                                   CGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTG                           <  SRR4302545.7772959/94‑1 (MQ=60)
                                                                   CGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTG                           >  SRR4302545.6663214/1‑94 (MQ=60)
                                                                    GCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGA                          <  SRR4302545.2586001/94‑1 (MQ=60)
                                                                     CTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAT                         <  SRR4302545.9195621/94‑1 (MQ=60)
                                                                       TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302545.1045982/94‑1 (MQ=60)
                                                                       TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302545.8630226/94‑1 (MQ=60)
                                                                        AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      <  SRR4302545.5009400/94‑1 (MQ=60)
                                                                        AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      >  SRR4302545.9219011/1‑94 (MQ=60)
                                                                         AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     >  SRR4302545.7121158/1‑94 (MQ=60)
                                                                         AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     >  SRR4302545.7121159/1‑94 (MQ=60)
                                                                          ACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTT                    >  SRR4302545.3767306/1‑94 (MQ=60)
                                                                          ACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTT                    <  SRR4302545.9083060/94‑1 (MQ=60)
                                                                          ACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTT                    <  SRR4302545.3994831/94‑1 (MQ=60)
                                                                           CCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTG                   <  SRR4302545.9339231/94‑1 (MQ=60)
                                                                           CCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTG                   <  SRR4302545.3407048/94‑1 (MQ=60)
                                                                           CCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTG                   >  SRR4302545.9113529/1‑94 (MQ=60)
                                                                           CCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTG                   <  SRR4302545.7452202/94‑1 (MQ=60)
                                                                            CGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGC                  <  SRR4302545.5242466/94‑1 (MQ=60)
                                                                             GGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCT                 <  SRR4302545.5489926/94‑1 (MQ=60)
                                                                             GGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCT                 >  SRR4302545.10918941/1‑94 (MQ=60)
                                                                             GGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCT                 <  SRR4302545.2298983/94‑1 (MQ=60)
                                                                              GGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTC                <  SRR4302545.9711835/94‑1 (MQ=60)
                                                                               GCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCC               >  SRR4302545.10228499/1‑94 (MQ=60)
                                                                               GCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCC               >  SRR4302545.3773964/1‑94 (MQ=60)
                                                                                CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              >  SRR4302545.5192798/1‑94 (MQ=60)
                                                                                CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              >  SRR4302545.5192799/1‑94 (MQ=60)
                                                                                 AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTA             <  SRR4302545.5699955/94‑1 (MQ=60)
                                                                                  AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            >  SRR4302545.8489422/1‑94 (MQ=60)
                                                                                  AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            <  SRR4302545.7076437/94‑1 (MQ=60)
                                                                                  AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            >  SRR4302545.2341336/1‑94 (MQ=60)
                                                                                   ACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTT           >  SRR4302545.7846604/1‑94 (MQ=60)
                                                                                   ACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTA           <  SRR4302545.8283641/94‑1 (MQ=60)
                                                                                   ACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTA           <  SRR4302545.1396632/94‑1 (MQ=60)
                                                                                   ACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTA           <  SRR4302545.3084031/94‑1 (MQ=60)
                                                                                    CGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAT          >  SRR4302545.6044566/1‑94 (MQ=60)
                                                                                    CGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAT          >  SRR4302545.3742494/1‑94 (MQ=60)
                                                                                      TGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302545.6803441/94‑1 (MQ=60)
                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        >  SRR4302545.10030872/1‑94 (MQ=60)
                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        >  SRR4302545.7847131/1‑94 (MQ=60)
                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        >  SRR4302545.7803359/1‑94 (MQ=60)
                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        >  SRR4302545.6412402/1‑94 (MQ=60)
                                                                                      CGCCAGCGACGTTGCGCTGGATCGCGTGCTACATGTGCCACAAATCTGGATTCCGTGCCAATTAGCTTCGTTTTGATAGTTTGCTCATTTATTG        >  SRR4302545.7803358/1‑94 (MQ=55)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       <  SRR4302545.2634884/94‑1 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302545.10871292/1‑94 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302545.638293/1‑94 (MQ=60)
                                                                                       GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       >  SRR4302545.581941/1‑94 (MQ=60)
                                                                                         CAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGC     >  SRR4302545.4601898/1‑94 (MQ=60)
                                                                                          AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    <  SRR4302545.6443045/94‑1 (MQ=60)
                                                                                          AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    >  SRR4302545.72400/1‑94 (MQ=60)
                                                                                           GCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCT   <  SRR4302545.7023386/94‑1 (MQ=60)
                                                                                           GCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCT   <  SRR4302545.4790008/94‑1 (MQ=60)
                                                                                            CGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  <  SRR4302545.343400/94‑1 (MQ=60)
                                                                                            |                                                                                             
GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279105‑3279290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑