Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,279,197	A→C	R145S (AGA→AGC)	agaA →	putative N‑acetylgalactosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,279,197	0	A	C	99.6%	948.1 / ‑4.8	274	R145S (AGA→AGC)	agaA	putative N‑acetylgalactosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (126/147); minor base T (0/1); total (126/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 282 total aligned reads displayed.

GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG  >  NC_000913/3279107‑3279286

ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         <  2:2363321/93‑1 (MQ=255)

ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         <  1:108266/93‑1 (MQ=255)

ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         >  2:337092/1‑93 (MQ=255)

ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         >  2:2310957/1‑93 (MQ=255)

ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         <  2:5066069/93‑1 (MQ=255)

   aGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGcgtcgt                                                                                      >  2:1210217/1‑93 (MQ=255)

   aGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGcgtcgt                                                                                      >  1:2491960/1‑93 (MQ=255)

    gCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt                                                                                     >  1:2162022/1‑93 (MQ=255)

     ccatccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTg                                                                                    >  2:3297640/1‑93 (MQ=255)

        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 >  2:4159024/1‑93 (MQ=255)

         ccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt                                                                                <  1:5306179/93‑1 (MQ=255)

          catATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTg                                                                               <  1:6364358/93‑1 (MQ=255)

           atatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg                                                                              <  1:685034/93‑1 (MQ=255)

           atatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg                                                                              >  1:4955453/1‑93 (MQ=255)

            tatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa                                                                             <  2:2479997/93‑1 (MQ=255)

            tatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa                                                                             <  1:5414071/93‑1 (MQ=255)

                gCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc                                                                         <  1:4537075/93‑1 (MQ=255)

                gCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc                                                                         >  2:2885184/1‑93 (MQ=255)

                 cGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCg                                                                        <  1:2597090/93‑1 (MQ=255)

                 cGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCg                                                                        <  1:4701876/93‑1 (MQ=255)

                  gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg                                                                       <  2:5797685/93‑1 (MQ=255)

                   tCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg                                                                      >  2:6379190/1‑93 (MQ=255)

                     gCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCt                                                                    <  2:3882053/93‑1 (MQ=255)

                      cTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa                                                                   >  2:4081852/1‑93 (MQ=255)

                          gtCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtg                                                               <  2:1076985/92‑1 (MQ=255)

                           tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtgt                                                              >  1:2018136/1‑93 (MQ=255)

                           tCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAtgt                                                              >  2:3738880/1‑93 (MQ=255)

                                cgcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac                                                         <  2:1783309/93‑1 (MQ=255)

                                 gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca                                                        >  1:352149/1‑93 (MQ=255)

                                 gcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaaca                                                        >  2:5477307/1‑93 (MQ=255)

                                   gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGGGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa                                                      <  1:4350059/93‑1 (MQ=255)

                                   gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa                                                      >  2:562150/1‑93 (MQ=255)

                                   gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa                                                      <  1:4213900/93‑1 (MQ=255)

                                   gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa                                                      <  2:1949180/93‑1 (MQ=255)

                                   gAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACaaa                                                      >  2:4975157/1‑93 (MQ=255)

                                     aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATc                                                    <  1:581265/93‑1 (MQ=255)

                                      tGCTGGGTGTTGATGGTGTTCTGCGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   <  1:3675832/93‑1 (MQ=255)

                                       gCTGGGTGTTGATTGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTg                                                  <  2:5997991/93‑1 (MQ=255)

                                        cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg                                                 >  2:706130/1‑93 (MQ=255)

                                         tGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa                                                >  1:5615334/1‑93 (MQ=255)

                                          gggTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAt                                               >  1:5682533/1‑93 (MQ=255)

                                             tgtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa                                            >  1:4185681/1‑93 (MQ=255)

                                             tgtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCa                                            >  2:2694334/1‑93 (MQ=255)

                                              gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           >  1:4337544/1‑93 (MQ=255)

                                                tGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAggg                                         <  2:2813867/93‑1 (MQ=255)

                                                 gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt                                        >  1:1281629/1‑93 (MQ=255)

                                                   tGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCa                                      >  1:3301289/1‑93 (MQ=255)

                                                    ggTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCaa                                     >  2:1565755/1‑93 (MQ=255)

                                                       gtTCTGGGATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa                                  <  1:2863667/93‑1 (MQ=255)

                                                        ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAg                                 >  2:3992000/1‑93 (MQ=255)

                                                         tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGc                                >  2:166109/1‑93 (MQ=255)

                                                          cTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt                               <  1:6182970/93‑1 (MQ=255)

                                                           tGGGATCGCTTAAACCGGGCGAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTCCATGTGCAACAAATCTGGATTCAGGGTCAATTAGCtt                              <  1:2740996/93‑1 (MQ=255)

                                                           tGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCtt                              >  1:5507098/1‑93 (MQ=255)

                                                           tGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCtt                              >  2:3132539/1‑93 (MQ=255)

                                                            gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc                             <  2:3832203/93‑1 (MQ=255)

                                                            gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc                             <  2:209660/93‑1 (MQ=255)

                                                             ggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCg                            <  1:1788790/93‑1 (MQ=255)

                                                               aTCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtt                          <  2:3573478/93‑1 (MQ=255)

                                                               aTCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtt                          <  1:1897653/93‑1 (MQ=255)

                                                                tCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt                         <  2:796246/93‑1 (MQ=255)

                                                                tCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt                         <  2:4867222/93‑1 (MQ=255)

                                                                tCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGttt                         >  2:3440058/1‑93 (MQ=255)

                                                                 cGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtttt                        >  2:2644664/1‑93 (MQ=255)

                                                                  gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTg                       >  2:2802569/1‑93 (MQ=255)

                                                                  gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTg                       >  1:3163998/1‑93 (MQ=255)

                                                                   cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGa                      <  1:1577520/93‑1 (MQ=255)

                                                                   cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGa                      >  2:1307013/1‑93 (MQ=255)

                                                                     tAACCCGGGAAACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    <  2:1127565/93‑1 (MQ=255)

                                                                     tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    <  1:5231943/93‑1 (MQ=255)

                                                                     tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    <  2:392238/93‑1 (MQ=255)

                                                                       aaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGt                  >  2:5210932/1‑93 (MQ=255)

                                                                       aaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGt                  >  2:3032088/1‑93 (MQ=255)

                                                                       aaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGt                  >  1:6049338/1‑93 (MQ=255)

                                                                       aaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGt                  >  2:4654927/1‑93 (MQ=255)

                                                                        aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGtt                 >  1:1385017/1‑93 (MQ=255)

                                                                        aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGtt                 >  2:4737458/1‑93 (MQ=255)

                                                                        aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGtt                 >  2:4563737/1‑93 (MQ=255)

                                                                        aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGtt                 <  1:4782648/93‑1 (MQ=255)

                                                                        aCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGtt                 <  1:5796543/93‑1 (MQ=255)

                                                                         ccGGGCAAACACGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGttt                >  2:2993700/1‑93 (MQ=255)

                                                                          cGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTg               <  1:334835/93‑1 (MQ=255)

                                                                           gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              <  1:2204685/93‑1 (MQ=255)

                                                                           gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              >  1:2964205/1‑93 (MQ=255)

                                                                             gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTc            <  1:2557339/93‑1 (MQ=255)

                                                                               aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCt          >  1:1500084/1‑93 (MQ=255)

                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         <  1:4432972/93‑1 (MQ=255)

                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         >  1:2816133/1‑93 (MQ=255)

                                                                                 aCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCAGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCttt        >  2:2148810/1‑93 (MQ=255)

                                                                                   gcgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAt      >  2:5371035/1‑93 (MQ=255)

                                                                                   gcgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAt      >  1:3786610/1‑93 (MQ=255)

                                                                                    cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAtt     >  2:1191298/1‑93 (MQ=255)

                                                                                     gcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTg    >  2:542333/1‑93 (MQ=255)

                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   <  1:465216/93‑1 (MQ=255)

                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   >  2:1479872/1‑93 (MQ=255)

                                                                                       cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTggg  >  1:2350850/1‑93 (MQ=255)

                                                                                       cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTggg  >  2:5757454/1‑93 (MQ=255)

                                                                                       cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTggg  >  1:1824396/1‑93 (MQ=255)

                                                                                       cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTggg  <  1:3880956/93‑1 (MQ=255)

                                                                                       cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTggg  >  1:3953501/1‑93 (MQ=255)

GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG  >  NC_000913/3279107‑3279286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279104‑3279290

TGCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGC                                                                                               <  SRR4302537.5145147/94‑1 (MQ=60)

 GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCG                                                                                              <  SRR4302537.46327/94‑1 (MQ=60)

   GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            <  SRR4302537.109058/94‑1 (MQ=60)

   GGAAGCCATCCATATGGCGTCGCTGCAGCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            <  SRR4302537.2259536/94‑1 (MQ=60)

     AAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGT                                                                                          >  SRR4302537.2509507/1‑94 (MQ=60)

      AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         >  SRR4302537.2177260/1‑94 (MQ=60)

      AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         <  SRR4302537.1776542/94‑1 (MQ=60)

       GCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        >  SRR4302537.3808476/1‑94 (MQ=60)

       GCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        >  SRR4302537.1364982/1‑94 (MQ=60)

       GCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGCTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        >  SRR4302537.4701295/1‑94 (MQ=60)

        CCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGC                                                                                       <  SRR4302537.5830804/94‑1 (MQ=60)

         CATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCG                                                                                      <  SRR4302537.4429841/94‑1 (MQ=60)

         CATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCG                                                                                      >  SRR4302537.3370749/1‑94 (MQ=60)

         CATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCG                                                                                      >  SRR4302537.5336987/1‑94 (MQ=60)

          ATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGC                                                                                     <  SRR4302537.5599386/94‑1 (MQ=60)

            CCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTG                                                                                   <  SRR4302537.5342645/94‑1 (MQ=60)

             CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  >  SRR4302537.4989618/1‑94 (MQ=60)

             CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  >  SRR4302537.2406728/1‑94 (MQ=60)

             CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  >  SRR4302537.5877946/1‑94 (MQ=60)

             CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  <  SRR4302537.6407771/94‑1 (MQ=60)

             CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  >  SRR4302537.4376526/1‑94 (MQ=60)

              ATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGA                                                                                 <  SRR4302537.689736/94‑1 (MQ=60)

               TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                <  SRR4302537.935003/94‑1 (MQ=60)

               TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                <  SRR4302537.2186523/94‑1 (MQ=60)

               TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                <  SRR4302537.4370675/94‑1 (MQ=60)

               TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                <  SRR4302537.5450983/94‑1 (MQ=60)

               TATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAT                                                                                <  SRR4302537.6274493/94‑1 (MQ=60)

                ATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGCGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATA                                                                               >  SRR4302537.3633768/1‑94 (MQ=60)

                  GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             <  SRR4302537.2520123/94‑1 (MQ=60)

                   GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302537.1405879/94‑1 (MQ=60)

                   GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302537.4568487/94‑1 (MQ=60)

                   GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302537.6424589/94‑1 (MQ=60)

                    CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302537.2834759/94‑1 (MQ=60)

                    CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302537.2615255/94‑1 (MQ=60)

                    CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302537.4734402/94‑1 (MQ=60)

                    CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302537.1274848/94‑1 (MQ=60)

                     GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          >  SRR4302537.4541204/1‑94 (MQ=60)

                     GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          >  SRR4302537.1918967/1‑94 (MQ=60)

                      TCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGC                                                                         <  SRR4302537.4879958/94‑1 (MQ=60)

                       CGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCT                                                                        <  SRR4302537.403734/94‑1 (MQ=60)

                       CGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCT                                                                        >  SRR4302537.5841872/1‑94 (MQ=60)

                        GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       >  SRR4302537.3156621/1‑94 (MQ=60)

                         CTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAC                                                                      >  SRR4302537.5013924/1‑94 (MQ=60)

                           GCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    <  SRR4302537.4543219/94‑1 (MQ=60)

                           GCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    <  SRR4302537.5480733/94‑1 (MQ=60)

                             CTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCAAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  <  SRR4302537.2148333/94‑1 (MQ=60)

                             ATCCGGCGCGAATGCTGGGTGTTGATGGTTTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  <  SRR4302537.3351938/94‑1 (MQ=60)

                             ATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  >  SRR4302537.2032206/1‑94 (MQ=60)

                              TCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTG                                                                 <  SRR4302537.584409/94‑1 (MQ=60)

                                 GGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAAGCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAA                                                              >  SRR4302537.3489503/1‑94 (MQ=60)

                                  GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302537.5567700/94‑1 (MQ=60)

                                  GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             >  SRR4302537.1823529/1‑94 (MQ=60)

                                   GGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACA                                                            <  SRR4302537.3940011/94‑1 (MQ=60)

                                   CGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACA                                                            >  SRR4302537.354594/1‑94 (MQ=60)

                                   CGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACA                                                            <  SRR4302537.3847092/94‑1 (MQ=60)

                                    GCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAA                                                           <  SRR4302537.1622356/94‑1 (MQ=60)

                                     CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          >  SRR4302537.535562/1‑94 (MQ=60)

                                     CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          <  SRR4302537.2608037/94‑1 (MQ=60)

                                      GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGGGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         <  SRR4302537.4380198/94‑1 (MQ=60)

                                      GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         <  SRR4302537.4243287/94‑1 (MQ=60)

                                       AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        >  SRR4302537.473636/1‑94 (MQ=60)

                                        ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       <  SRR4302537.585246/94‑1 (MQ=60)

                                         TGCTGGGTGTTGATGGTGTTCTGCGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      <  SRR4302537.3701686/94‑1 (MQ=60)

                                           CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    >  SRR4302537.5653727/1‑94 (MQ=60)

                                           CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    >  SRR4302537.2652101/1‑94 (MQ=60)

                                            TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   <  SRR4302537.4637055/94‑1 (MQ=60)

                                            TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   >  SRR4302537.5721398/1‑94 (MQ=60)

                                             GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  <  SRR4302537.1877395/94‑1 (MQ=60)

                                             GGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATT                                                  >  SRR4302537.5694541/1‑94 (MQ=60)

                                               GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                >  SRR4302537.4214903/1‑94 (MQ=60)

                                               GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                <  SRR4302537.253160/94‑1 (MQ=60)

                                               GTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCA                                                <  SRR4302537.2199812/94‑1 (MQ=60)

                                                TGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               >  SRR4302537.4367652/1‑94 (MQ=60)

                                                GGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAAAGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               <  SRR4302537.1447722/94‑1 (MQ=60)

                                                 GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302537.1905663/94‑1 (MQ=60)

                                                 GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302537.4210924/94‑1 (MQ=60)

                                                  TTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             <  SRR4302537.4894529/94‑1 (MQ=60)

                                                   TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            <  SRR4302537.479181/94‑1 (MQ=60)

                                                   TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            >  SRR4302537.1290538/1‑94 (MQ=60)

                                                   TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            >  SRR4302537.2826206/1‑94 (MQ=60)

                                                    GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           <  SRR4302537.6375808/94‑1 (MQ=60)

                                                    GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           >  SRR4302537.4285847/1‑94 (MQ=60)

                                                     ATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCA                                          >  SRR4302537.3324630/1‑94 (MQ=60)

                                                         TGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTA                                      <  SRR4302537.2934658/94‑1 (MQ=60)

                                                          GTTCTGGGATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     <  SRR4302537.2883743/94‑1 (MQ=60)

                                                          GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     <  SRR4302537.6202417/94‑1 (MQ=60)

                                                          GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     >  SRR4302537.4438821/1‑94 (MQ=60)

                                                             CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  <  SRR4302537.6225557/94‑1 (MQ=60)

                                                             CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  >  SRR4302537.367262/1‑94 (MQ=60)

                                                             CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  >  SRR4302537.5544710/1‑94 (MQ=60)

                                                              TGGGATCGCTTAAACCGGGCGAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTCCATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 <  SRR4302537.2760118/94‑1 (MQ=60)

                                                              TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 >  SRR4302537.2858028/1‑94 (MQ=60)

                                                              TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 >  SRR4302537.5477205/1‑94 (MQ=60)

                                                               GGGATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                <  SRR4302537.2304667/94‑1 (MQ=60)

                                                                GGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGT                               <  SRR4302537.1801287/94‑1 (MQ=60)

                                                                 GATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTT                              <  SRR4302537.158853/94‑1 (MQ=60)

                                                                  ATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTT                             <  SRR4302537.1910837/94‑1 (MQ=60)

                                                                   TCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            >  SRR4302537.2947514/1‑94 (MQ=60)

                                                                   TCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            <  SRR4302537.5157663/94‑1 (MQ=60)

                                                                   TCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTT                            >  SRR4302537.5683498/1‑94 (MQ=60)

                                                                    CGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTG                           >  SRR4302537.3186215/1‑94 (MQ=60)

                                                                     GCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGA                          >  SRR4302537.2674715/1‑94 (MQ=60)

                                                                     GCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGA                          >  SRR4302537.3953856/1‑94 (MQ=60)

                                                                      CTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAT                         <  SRR4302537.1588524/94‑1 (MQ=60)

                                                                        TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302537.4230290/94‑1 (MQ=60)

                                                                        TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302537.5267930/94‑1 (MQ=60)

                                                                         AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      >  SRR4302537.6090883/1‑94 (MQ=60)

                                                                          AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     <  SRR4302537.3894138/94‑1 (MQ=60)

                                                                          AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     >  SRR4302537.1394678/1‑94 (MQ=60)

                                                                          AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     <  SRR4302537.2585455/94‑1 (MQ=60)

                                                                           ACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTT                    <  SRR4302537.5836284/94‑1 (MQ=60)

                                                                           ACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTT                    <  SRR4302537.4815664/94‑1 (MQ=60)

                                                                            CCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTG                   >  SRR4302537.1584308/1‑94 (MQ=60)

                                                                             CGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGC                  <  SRR4302537.337151/94‑1 (MQ=60)

                                                                             CGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGC                  <  SRR4302537.6155325/94‑1 (MQ=60)

                                                                             CGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGC                  >  SRR4302537.2985009/1‑94 (MQ=60)

                                                                              GGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCT                 >  SRR4302537.2008808/1‑94 (MQ=60)

                                                                              GGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCT                 <  SRR4302537.2220231/94‑1 (MQ=60)

                                                                                GCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCC               <  SRR4302537.2575265/94‑1 (MQ=60)

                                                                                 CAAACGCGCCATCTTCGTTGCGCTGGAGACGGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              <  SRR4302537.4258248/94‑1 (MQ=60)

                                                                                 CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              <  SRR4302537.1305056/94‑1 (MQ=60)

                                                                                 CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              >  SRR4302537.1510554/1‑94 (MQ=60)

                                                                                  AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTT             >  SRR4302537.5800383/1‑94 (MQ=60)

                                                                                  AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTT             >  SRR4302537.2835824/1‑94 (MQ=60)

                                                                                   AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            <  SRR4302537.3526979/94‑1 (MQ=60)

                                                                                   AACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            <  SRR4302537.4463585/94‑1 (MQ=60)

                                                                                    ACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTA           <  SRR4302537.4684016/94‑1 (MQ=60)

                                                                                    ACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTA           <  SRR4302537.3157026/94‑1 (MQ=60)

                                                                                     CGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAT          >  SRR4302537.3813197/1‑94 (MQ=60)

                                                                                       CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302537.212036/94‑1 (MQ=60)

                                                                                       CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302537.328017/94‑1 (MQ=60)

                                                                                       CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        >  SRR4302537.5274474/1‑94 (MQ=60)

                                                                                        GCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGG       <  SRR4302537.6469550/94‑1 (MQ=60)

                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      >  SRR4302537.3981155/1‑94 (MQ=60)

                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      >  SRR4302537.2367401/1‑94 (MQ=60)

                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      >  SRR4302537.1837113/1‑94 (MQ=60)

                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      <  SRR4302537.3251187/94‑1 (MQ=60)

                                                                                         CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      <  SRR4302537.468418/94‑1 (MQ=60)

                                                                                          CAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGC     >  SRR4302537.6202963/1‑94 (MQ=60)

                                                                                          CAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGC     <  SRR4302537.3796742/94‑1 (MQ=60)

                                                                                          CAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGC     <  SRR4302537.3908102/94‑1 (MQ=60)

                                                                                           AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    >  SRR4302537.2709388/1‑94 (MQ=60)

                                                                                           AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    >  SRR4302537.5598356/1‑94 (MQ=60)

                                                                                           AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    >  SRR4302537.2412253/1‑94 (MQ=60)

                                                                                            GCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCT   >  SRR4302537.4031692/1‑94 (MQ=60)

                                                                                             CGTCGTTGCGCTGGATAGTGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  SRR4302537.2339068/1‑94 (MQ=60)

                                                                                             CGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  SRR4302537.4543058/1‑94 (MQ=60)

                                                                                             CGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  <  SRR4302537.4457473/94‑1 (MQ=60)

TGCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279104‑3279290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`