Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,311,437 T→C N740S (AAC→AGC)  infB ← protein chain initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,311,4370TC99.0% 1736.7 / 1.8 506N740S (AAC→AGC) infBprotein chain initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (268/233);  total (271/235)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

Only 100 of 529 total aligned reads displayed.

GACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTT  >  NC_000913/3311346‑3311527
                                                                                           |                                                                                          
 aCGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTgg                                                                                         <  2:8796479/94‑1 (MQ=255)
   ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAc                                                                                       >  1:8266110/1‑94 (MQ=255)
     agtagtAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAcgc                                                                                     >  1:8586284/1‑94 (MQ=255)
      gtagtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAcgcc                                                                                    >  1:6893189/1‑94 (MQ=255)
       tagtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGAcgccg                                                                                   >  1:2334161/1‑94 (MQ=255)
         gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCa                                                                                 <  2:9612450/94‑1 (MQ=255)
          taACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAg                                                                                <  1:1201211/94‑1 (MQ=255)
           aaCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGc                                                                               <  1:655827/94‑1 (MQ=255)
           aaCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGc                                                                               <  2:8119149/94‑1 (MQ=255)
            aCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGcc                                                                              >  2:971857/1‑94 (MQ=255)
             cGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCa                                                                             >  1:1839684/1‑94 (MQ=255)
                aGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAggg                                                                          >  1:10004148/1‑94 (MQ=255)
                aGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAggg                                                                          >  2:3937182/1‑94 (MQ=255)
                  aTCCAGGCTTTCCGCTACAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTg                                                                        <  2:5951132/94‑1 (MQ=255)
                   tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTgg                                                                       <  1:928691/94‑1 (MQ=255)
                   tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTgg                                                                       >  2:1600026/1‑94 (MQ=255)
                   tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTgg                                                                       >  2:2868780/1‑94 (MQ=255)
                   tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTgg                                                                       >  1:4527722/1‑94 (MQ=255)
                    ccAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGc                                                                      >  2:10012062/1‑94 (MQ=255)
                     cAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCg                                                                     >  1:9565162/1‑94 (MQ=255)
                      aGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGt                                                                    >  2:5233404/1‑94 (MQ=255)
                       ggCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTc                                                                   >  2:752738/1‑94 (MQ=255)
                       ggCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTc                                                                   >  1:4768098/1‑94 (MQ=255)
                        gCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCg                                                                  <  1:629318/94‑1 (MQ=255)
                        gCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCg                                                                  >  1:2003052/1‑94 (MQ=255)
                        gCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCg                                                                  <  1:1428164/94‑1 (MQ=255)
                         cTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCgg                                                                 >  2:7289992/1‑94 (MQ=255)
                          tttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGt                                                                >  1:10244927/1‑94 (MQ=255)
                           ttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGtt                                                               >  1:7129367/1‑94 (MQ=255)
                           ttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGtt                                                               >  1:8906525/1‑94 (MQ=255)
                            tCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGttt                                                              <  1:3667843/94‑1 (MQ=255)
                            tCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGgtt                                                              >  2:1906851/1‑94 (MQ=255)
                              cGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCg                                                            <  1:746857/94‑1 (MQ=255)
                              cGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCg                                                            >  1:668043/1‑94 (MQ=255)
                                   cAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGAt                                                       >  2:3670010/1‑94 (MQ=255)
                                      tCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacc                                                    >  2:6871690/1‑94 (MQ=255)
                                       cACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacca                                                   >  1:9842407/1‑94 (MQ=255)
                                        aCTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccac                                                  <  1:7851714/94‑1 (MQ=255)
                                          tttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCt                                                <  2:6162437/94‑1 (MQ=255)
                                          cttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCt                                                <  2:2706111/93‑1 (MQ=255)
                                             aCGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACg                                             <  1:4447732/94‑1 (MQ=255)
                                             aCGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACg                                             <  2:5717216/94‑1 (MQ=255)
                                             aCGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACg                                             >  1:2577701/1‑94 (MQ=255)
                                              cGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGc                                            >  2:10381368/1‑94 (MQ=255)
                                              cGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGc                                            <  1:8689232/94‑1 (MQ=255)
                                              cGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGc                                            >  2:2124193/1‑94 (MQ=255)
                                               gTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGcc                                           <  2:5528957/94‑1 (MQ=255)
                                                tGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCa                                          >  1:1681182/1‑94 (MQ=255)
                                                tGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCa                                          <  1:3340523/94‑1 (MQ=255)
                                                 gCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAg                                         >  1:2669870/1‑94 (MQ=255)
                                                  cAGAGGCATCCGCACGTACGTTAAAGGCAACCAGGGTGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACATACGCCAGa                                        >  1:5055774/1‑94 (MQ=255)
                                                  cAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGGTTCGGTGATACCACCTACGCCAGa                                        >  2:713556/1‑94 (MQ=255)
                                                   agagGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGaa                                       <  2:340128/94‑1 (MQ=255)
                                                    gagGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAc                                      <  1:773011/94‑1 (MQ=255)
                                                     agGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCGGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc                                     <  1:5880233/94‑1 (MQ=255)
                                                     agGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc                                     <  1:6101922/94‑1 (MQ=255)
                                                      ggCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCg                                    >  1:10596409/1‑94 (MQ=255)
                                                       gCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCga                                   <  2:4258202/94‑1 (MQ=255)
                                                       gCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCga                                   >  2:10088858/1‑94 (MQ=255)
                                                        cATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat                                  <  2:4829993/94‑1 (MQ=255)
                                                        cATCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat                                  >  1:5384573/1‑94 (MQ=255)
                                                         aTCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatg                                 <  2:9073263/94‑1 (MQ=255)
                                                         aTCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatg                                 >  1:7609136/1‑94 (MQ=255)
                                                          tCAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatga                                <  2:1136141/94‑1 (MQ=255)
                                                           cAGCACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatgat                               >  1:9110915/1‑94 (MQ=255)
                                                              cACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCtt                            <  1:10128176/94‑1 (MQ=255)
                                                              cACGTACGTTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCtt                            <  1:2923523/94‑1 (MQ=255)
                                                                cgtacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCa                          <  1:5735417/94‑1 (MQ=255)
                                                                 gtacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                         >  2:2432163/1‑94 (MQ=255)
                                                                 gtacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                         >  2:7596356/1‑94 (MQ=255)
                                                                 gtacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                         <  2:675002/94‑1 (MQ=255)
                                                                  tacgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACt                        <  2:4496061/94‑1 (MQ=255)
                                                                    cgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACTACCTACGCCAGAACCGATGATCTTCACttt                      <  1:7290805/94‑1 (MQ=255)
                                                                    cgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACttt                      >  2:10580756/1‑94 (MQ=255)
                                                                    cgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACttt                      <  1:409355/94‑1 (MQ=255)
                                                                    cgtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACttt                      >  1:486372/1‑94 (MQ=255)
                                                                     gtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTa                     <  2:3275689/94‑1 (MQ=255)
                                                                     gtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTa                     <  2:7846119/94‑1 (MQ=255)
                                                                     gtTAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTa                     >  1:7988023/1‑94 (MQ=255)
                                                                       tAAAGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTGGGTGATACCACATCCGCCAGACCCGATGATCTTCACTTTAAc                   >  2:6079734/1‑94 (MQ=255)
                                                                        aaaGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACt                  >  2:2591397/1‑94 (MQ=255)
                                                                        aaaGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACt                  >  2:23089/1‑94 (MQ=255)
                                                                          aGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGGTACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTc                <  1:2464327/94‑1 (MQ=255)
                                                                          aGCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTc                >  2:663096/1‑94 (MQ=255)
                                                                           gCCAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCg               <  1:8030121/94‑1 (MQ=255)
                                                                           agcAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCg               <  2:5033967/92‑1 (MQ=255)
                                                                             cAACCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTc             <  2:6360718/94‑1 (MQ=255)
                                                                              aaCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCa            >  1:5590564/1‑94 (MQ=255)
                                                                               aCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAg           <  1:5166561/94‑1 (MQ=255)
                                                                               aCCAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAg           <  1:10386665/94‑1 (MQ=255)
                                                                                ccAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGt          <  2:1859640/94‑1 (MQ=255)
                                                                                ccAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGt          <  1:3883578/94‑1 (MQ=255)
                                                                                ccAGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGt          <  2:9392210/94‑1 (MQ=255)
                                                                                  aGGATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAg        >  2:8523454/1‑94 (MQ=255)
                                                                                    gATGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGAc      >  1:3086897/1‑94 (MQ=255)
                                                                                     aTGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACa     >  2:7020113/1‑94 (MQ=255)
                                                                                      tGGCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAg    <  2:142303/94‑1 (MQ=255)
                                                                                       ggCGCTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGt   >  1:8362191/1‑94 (MQ=255)
                                                                                        gcgcTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGtt  <  1:1554386/94‑1 (MQ=255)
                                                                                        gcgcTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGtt  <  1:6973228/94‑1 (MQ=255)
                                                                                           |                                                                                          
GACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTT  >  NC_000913/3311346‑3311527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 335 total aligned reads displayed.
TCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGA  >  NC_000913/3325408‑3325594
                                                                                             |                                                                                             
TCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCC                                                                                               >  SRR4302545.1828499/1‑94 (MQ=60)
  CGAACGTAGGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTG                                                                                             >  SRR4302545.6649461/1‑94 (MQ=60)
  CCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTG                                                                                             <  SRR4302545.3973556/94‑1 (MQ=60)
  CCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTG                                                                                             >  SRR4302545.9004600/1‑94 (MQ=60)
   CAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGG                                                                                            >  SRR4302545.6483724/1‑94 (MQ=60)
   CAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGG                                                                                            >  SRR4302545.10618349/1‑94 (MQ=60)
   CAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGACTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGG                                                                                            >  SRR4302545.4018683/1‑94 (MQ=60)
    AACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGC                                                                                           >  SRR4302545.193506/1‑94 (MQ=60)
    AACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGC                                                                                           >  SRR4302545.10905512/1‑94 (MQ=60)
    AACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGC                                                                                           >  SRR4302545.7142260/1‑94 (MQ=60)
    AACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGC                                                                                           <  SRR4302545.2115780/94‑1 (MQ=60)
    AACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGC                                                                                           >  SRR4302545.10217339/1‑94 (MQ=60)
     ACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCA                                                                                          >  SRR4302545.8727899/1‑94 (MQ=60)
       GTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGT                                                                                        >  SRR4302545.2478657/1‑94 (MQ=60)
        TAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTA                                                                                       >  SRR4302545.5108322/1‑94 (MQ=60)
        TAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTA                                                                                       >  SRR4302545.8223889/1‑94 (MQ=60)
         AAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAA                                                                                      <  SRR4302545.1319344/94‑1 (MQ=60)
         AAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTCGCAGTAA                                                                                      >  SRR4302545.1455641/1‑94 (MQ=60)
          AGCAAAAAATATGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAAT                                                                                     >  SRR4302545.7099010/1‑94 (MQ=60)
          AGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAAT                                                                                     <  SRR4302545.7780452/94‑1 (MQ=60)
          AGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAAT                                                                                     <  SRR4302545.335168/94‑1 (MQ=60)
            CAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGG                                                                                   <  SRR4302545.9845833/94‑1 (MQ=60)
            AAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGG                                                                                   <  SRR4302545.7278111/94‑1 (MQ=60)
             AAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGT                                                                                  >  SRR4302545.6554748/1‑94 (MQ=60)
             AAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGT                                                                                  >  SRR4302545.4278234/1‑94 (MQ=60)
             AAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGT                                                                                  >  SRR4302545.963329/1‑94 (MQ=60)
             AAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGT                                                                                  <  SRR4302545.2713024/94‑1 (MQ=60)
             AAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGT                                                                                  >  SRR4302545.4957870/1‑94 (MQ=60)
              AAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTT                                                                                 <  SRR4302545.2822078/94‑1 (MQ=60)
              AAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTT                                                                                 >  SRR4302545.2520552/1‑94 (MQ=60)
               AAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTT                                                                                >  SRR4302545.3011799/1‑94 (MQ=60)
               AAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTT                                                                                <  SRR4302545.9789606/94‑1 (MQ=60)
               AAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTT                                                                                <  SRR4302545.5738482/94‑1 (MQ=60)
               AAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTT                                                                                >  SRR4302545.5653670/1‑94 (MQ=60)
                AAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTG                                                                               >  SRR4302545.3145128/1‑94 (MQ=60)
                AAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTG                                                                               >  SRR4302545.2533814/1‑94 (MQ=60)
                 AATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGA                                                                              >  SRR4302545.8264888/1‑94 (MQ=60)
                 AATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGA                                                                              >  SRR4302545.7243537/1‑94 (MQ=60)
                  ATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGAC                                                                             <  SRR4302545.10506347/94‑1 (MQ=60)
                  ATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGCCTGGAACATCCGTTCAACCAAGTTGTTCTGCCTGCCGGTAATGGTTGGAC                                                                             >  SRR4302545.771936/1‑94 (MQ=55)
                   TACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACC                                                                            <  SRR4302545.1717988/94‑1 (MQ=60)
                    ACGATGAATCTGAGTACTAAACATAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCG                                                                           <  SRR4302545.7796520/94‑1 (MQ=60)
                    ACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCG                                                                           >  SRR4302545.7141720/1‑94 (MQ=60)
                    ACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCG                                                                           <  SRR4302545.2949394/94‑1 (MQ=60)
                    ACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCG                                                                           <  SRR4302545.4563940/94‑1 (MQ=60)
                    ACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCG                                                                           <  SRR4302545.5797675/94‑1 (MQ=60)
                      GATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAA                                                                         <  SRR4302545.2403203/94‑1 (MQ=60)
                      GATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAA                                                                         >  SRR4302545.6253980/1‑94 (MQ=60)
                       attattaagagtaataagCAAAAACAGCACCTGAAAGGTCTGTCTCATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAG                                                                        <  SRR4302545.6301054/76‑1 (MQ=60)
                       GTGAATCTGAGTACTAACCAAAAGCAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAG                                                                        <  SRR4302545.7445955/94‑1 (MQ=60)
                       ATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAG                                                                        >  SRR4302545.5746877/1‑94 (MQ=60)
                       ATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAG                                                                        <  SRR4302545.1307049/94‑1 (MQ=60)
                       ATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAG                                                                        <  SRR4302545.6512981/94‑1 (MQ=60)
                        TGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGG                                                                       <  SRR4302545.791478/94‑1 (MQ=60)
                        TGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGG                                                                       >  SRR4302545.10151479/1‑94 (MQ=60)
                         GAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTTGCAGTAATGGTTTGACCGAAGGG                                                                      >  SRR4302545.5958838/1‑94 (MQ=60)
                         GAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGG                                                                      >  SRR4302545.9825018/1‑94 (MQ=60)
                          AATCTGAGTACTAAACAAACACAGCACCTGAAAGGTCTGGCACCTCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTCATGGTTTGACCGAAAGGG                                                                     <  SRR4302545.9685303/94‑1 (MQ=60)
                          AATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGG                                                                     <  SRR4302545.2059874/94‑1 (MQ=60)
                          AATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGG                                                                     >  SRR4302545.9176812/1‑94 (MQ=60)
                           ATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACAGCAGGGGT                                                                    >  SRR4302545.8835320/1‑94 (MQ=60)
                           ATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACAGAAGGGGT                                                                    >  SRR4302545.8835319/1‑94 (MQ=60)
                            TCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTG                                                                   <  SRR4302545.10522363/94‑1 (MQ=60)
                            TCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTG                                                                   <  SRR4302545.8563888/94‑1 (MQ=60)
                            ACTGAGTACTAAACAAAAAAAGCACATGAAAGGTCTGGCACATCCGCTAAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTG                                                                   <  SRR4302545.8563890/94‑1 (MQ=60)
                             CTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGC                                                                  >  SRR4302545.8325994/1‑94 (MQ=60)
                              TGAGTACTAAACGAACACAGCACCTGAAAGGTCTGGCACATCCACTCGAGCCAGTTGTTCTGCCTGGCAGTAATGGATTGACCGAAGGGGAGCT                                                                 <  SRR4302545.9654464/94‑1 (MQ=60)
                              TGAGTACTAAACAAACACAGCACCTGAAAGGTCTGGCACATCCGCTCCAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCT                                                                 <  SRR4302545.9654465/94‑1 (MQ=60)
                              TGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCT                                                                 >  SRR4302545.3591190/1‑94 (MQ=60)
                               GAGTACTAGACGAAAACAGAACCTGACAGGTCTGGCACATCCGCTCAGGCCAGTTGTTCTGCCTGGCAGTAATGGTATGACCGAAGGGGTGCTG                                                                <  SRR4302545.9694102/94‑1 (MQ=60)
                               GAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTG                                                                >  SRR4302545.7688858/1‑94 (MQ=60)
                               GAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTG                                                                <  SRR4302545.1081951/94‑1 (MQ=60)
                               GAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTG                                                                <  SRR4302545.9375077/94‑1 (MQ=60)
                                AGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGG                                                               <  SRR4302545.567334/94‑1 (MQ=60)
                                AGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGG                                                               >  SRR4302545.8979670/1‑94 (MQ=60)
                                AGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGG                                                               >  SRR4302545.1131001/1‑94 (MQ=60)
                                AGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGG                                                               >  SRR4302545.3303353/1‑94 (MQ=60)
                                AGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGG                                                               <  SRR4302545.8713264/94‑1 (MQ=60)
                                AGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGG                                                               <  SRR4302545.4626143/94‑1 (MQ=60)
                                 GTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCCAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGC                                                              >  SRR4302545.7654474/1‑94 (MQ=60)
                                  TACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCC                                                             >  SRR4302545.8080524/1‑94 (MQ=60)
                                  TACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCC                                                             <  SRR4302545.8745088/94‑1 (MQ=60)
                                    CTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGA                                                           <  SRR4302545.7398636/94‑1 (MQ=60)
                                     TAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAG                                                          >  SRR4302545.4094845/1‑94 (MQ=60)
                                     TAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAG                                                          <  SRR4302545.4137104/94‑1 (MQ=60)
                                       AACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGAT                                                        >  SRR4302545.10341234/1‑94 (MQ=60)
                                       AACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGAT                                                        <  SRR4302545.10637411/94‑1 (MQ=60)
                                        ACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATT                                                       >  SRR4302545.4687602/1‑94 (MQ=60)
                                         CAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTG                                                      <  SRR4302545.343234/94‑1 (MQ=60)
                                          AAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGA                                                     >  SRR4302545.2125159/1‑94 (MQ=60)
                                          AAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGA                                                     >  SRR4302545.9469863/1‑94 (MQ=60)
                                           aaaacacccccctAAAGGTCCGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTTGCCGAGATTGAA                                                    <  SRR4302545.3078882/81‑1 (MQ=60)
                                           AAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAA                                                    >  SRR4302545.6442974/1‑94 (MQ=60)
                                           AAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAA                                                    >  SRR4302545.8397318/1‑94 (MQ=60)
                                            AAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAAC                                                   <  SRR4302545.1338790/94‑1 (MQ=60)
                                            AAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAAC                                                   <  SRR4302545.4305148/94‑1 (MQ=60)
                                             AACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACA                                                  <  SRR4302545.5258912/94‑1 (MQ=60)
                                             AACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACA                                                  <  SRR4302545.8453814/94‑1 (MQ=60)
                                             AACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACA                                                  <  SRR4302545.9431287/94‑1 (MQ=60)
                                             AACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACA                                                  <  SRR4302545.9741927/94‑1 (MQ=60)
                                             AACACCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACA                                                  <  SRR4302545.4554205/94‑1 (MQ=60)
                                              ACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAGTGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAA                                                 <  SRR4302545.4808425/94‑1 (MQ=60)
                                              ACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAA                                                 >  SRR4302545.4248111/1‑94 (MQ=60)
                                              ACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAA                                                 <  SRR4302545.1899655/94‑1 (MQ=60)
                                               CAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAG                                                <  SRR4302545.8807950/94‑1 (MQ=60)
                                               CAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAG                                                <  SRR4302545.5049376/94‑1 (MQ=60)
                                                 gcagagagagggctgaccccccccgccaaagctattttTTCTGCTGGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCG                                              <  SRR4302545.6051134/56‑1 (MQ=60)
                                                 GCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCG                                              >  SRR4302545.4877007/1‑94 (MQ=60)
                                                  CACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGT                                             >  SRR4302545.7292346/1‑94 (MQ=60)
                                                   ACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTT                                            >  SRR4302545.2259903/1‑94 (MQ=60)
                                                    CCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTA                                           >  SRR4302545.5364392/1‑94 (MQ=60)
                                                    CCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTA                                           <  SRR4302545.10457073/94‑1 (MQ=60)
                                                    CCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTA                                           <  SRR4302545.593372/94‑1 (MQ=60)
                                                     CTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAG                                          >  SRR4302545.2015302/1‑94 (MQ=60)
                                                     CTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAG                                          <  SRR4302545.10374167/94‑1 (MQ=60)
                                                     CTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAG                                          <  SRR4302545.4357447/94‑1 (MQ=60)
                                                     CTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAG                                          <  SRR4302545.7322245/94‑1 (MQ=60)
                                                     CTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAG                                          >  SRR4302545.9095110/1‑94 (MQ=60)
                                                     CTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAG                                          >  SRR4302545.1513668/1‑94 (MQ=60)
                                                     CTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAG                                          >  SRR4302545.10244769/1‑94 (MQ=60)
                                                      TGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGA                                         <  SRR4302545.9309094/94‑1 (MQ=60)
                                                      TGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGA                                         <  SRR4302545.9072101/94‑1 (MQ=60)
                                                      TGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGA                                         >  SRR4302545.1927995/1‑94 (MQ=60)
                                                        AAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGC                                       <  SRR4302545.6801002/94‑1 (MQ=60)
                                                        AAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGC                                       >  SRR4302545.287290/1‑94 (MQ=60)
                                                        AAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGC                                       <  SRR4302545.3796221/94‑1 (MQ=60)
                                                         AAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCA                                      <  SRR4302545.4979417/94‑1 (MQ=60)
                                                          AGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCAC                                     >  SRR4302545.4450938/1‑94 (MQ=60)
                                                           GGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACC                                    >  SRR4302545.10733066/1‑94 (MQ=60)
                                                           GGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACC                                    >  SRR4302545.2309008/1‑94 (MQ=60)
                                                             TCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCAT                                  <  SRR4302545.289798/94‑1 (MQ=60)
                                                              CTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATG                                 >  SRR4302545.5085940/1‑94 (MQ=60)
                                                              CTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATG                                 >  SRR4302545.9038550/1‑94 (MQ=60)
                                                              CTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATG                                 <  SRR4302545.344528/94‑1 (MQ=60)
                                                              CTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATG                                 <  SRR4302545.6548633/94‑1 (MQ=60)
                                                               TGTCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGA                                <  SRR4302545.4008431/94‑1 (MQ=60)
                                                               TGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGA                                <  SRR4302545.1147256/94‑1 (MQ=60)
                                                                GGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGCACAAGCGTTAGAGCACCATGAA                               <  SRR4302545.10707714/94‑1 (MQ=60)
                                                                GGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAA                               <  SRR4302545.9494363/94‑1 (MQ=60)
                                                                 GCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAAC                              <  SRR4302545.1494956/94‑1 (MQ=60)
                                                                 GCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAAC                              <  SRR4302545.3611234/94‑1 (MQ=60)
                                                                   ACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTC                            >  SRR4302545.8501639/1‑94 (MQ=60)
                                                                   ACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTC                            <  SRR4302545.10895648/94‑1 (MQ=60)
                                                                   ACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTC                            >  SRR4302545.7317104/1‑94 (MQ=60)
                                                                    CATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCgacatgaaatct                           >  SRR4302545.3922820/1‑82 (MQ=60)
                                                                    CATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCA                           <  SRR4302545.495977/94‑1 (MQ=60)
                                                                    CATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCA                           <  SRR4302545.3249140/94‑1 (MQ=60)
                                                                    CATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCA                           >  SRR4302545.4202612/1‑94 (MQ=60)
                                                                    CATCCGCTCAAGCCAGTTGTTCGGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCA                           <  SRR4302545.3004442/94‑1 (MQ=60)
                                                                     ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          >  SRR4302545.4936608/1‑94 (MQ=60)
                                                                     ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          >  SRR4302545.10868610/1‑94 (MQ=60)
                                                                     ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          >  SRR4302545.4405138/1‑94 (MQ=60)
                                                                     ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          >  SRR4302545.8734565/1‑94 (MQ=60)
                                                                     ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          >  SRR4302545.4850743/1‑94 (MQ=60)
                                                                      TCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATC                         <  SRR4302545.5303752/94‑1 (MQ=60)
                                                                       CCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCA                        >  SRR4302545.349019/1‑94 (MQ=60)
                                                                        CGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAA                       >  SRR4302545.821299/1‑94 (MQ=60)
                                                                        CGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAA                       >  SRR4302545.6725276/1‑94 (MQ=60)
                                                                        CGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAA                       >  SRR4302545.3442276/1‑94 (MQ=60)
                                                                        CGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAA                       >  SRR4302545.6987432/1‑94 (MQ=60)
                                                                          CTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGG                     >  SRR4302545.7295164/1‑94 (MQ=60)
                                                                            CAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTG                   >  SRR4302545.8656122/1‑94 (MQ=60)
                                                                            CAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTG                   <  SRR4302545.10014619/94‑1 (MQ=60)
                                                                            CAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTG                   <  SRR4302545.7230709/94‑1 (MQ=60)
                                                                            CAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTG                   <  SRR4302545.10763059/94‑1 (MQ=60)
                                                                             AAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGA                  <  SRR4302545.6998128/94‑1 (MQ=60)
                                                                             AAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGA                  <  SRR4302545.4722495/94‑1 (MQ=60)
                                                                             AAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGA                  >  SRR4302545.10789420/1‑94 (MQ=60)
                                                                              cgccggtttTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAACGTTAGAGCACCATGAACTCATCAAGGTCAA                 >  SRR4302545.6739090/10‑94 (MQ=60)
                                                                              AGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAA                 >  SRR4302545.7196157/1‑94 (MQ=60)
                                                                               GCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAA                <  SRR4302545.1554071/94‑1 (MQ=60)
                                                                               GCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAA                >  SRR4302545.3132624/1‑94 (MQ=60)
                                                                               GCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAA                >  SRR4302545.4430878/1‑94 (MQ=60)
                                                                               GCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAA                <  SRR4302545.8930829/94‑1 (MQ=60)
                                                                                CCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAA               <  SRR4302545.10250201/94‑1 (MQ=60)
                                                                                 CAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAT              <  SRR4302545.2771485/94‑1 (MQ=60)
                                                                                 CAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAT              <  SRR4302545.3635506/94‑1 (MQ=60)
                                                                                 CAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAT              <  SRR4302545.8722976/94‑1 (MQ=60)
                                                                                  AGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGCACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATC             >  SRR4302545.10293845/1‑94 (MQ=60)
                                                                                  AGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATC             >  SRR4302545.3682459/1‑94 (MQ=60)
                                                                                  AGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATC             >  SRR4302545.7906800/1‑94 (MQ=60)
                                                                                    TTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGC           >  SRR4302545.6117228/1‑94 (MQ=60)
                                                                                     TGTTCTGCCTGGCAGTAAGGGTTTGACCGAAGGGGTGCTGGCCGAGTTTGTACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCC          >  SRR4302545.9658832/1‑94 (MQ=60)
                                                                                      GTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCA         <  SRR4302545.9055632/94‑1 (MQ=60)
                                                                                      GTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCA         >  SRR4302545.1184777/1‑94 (MQ=60)
                                                                                      GTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCA         >  SRR4302545.3215356/1‑94 (MQ=60)
                                                                                        TCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTTAAAATCGCCACC       <  SRR4302545.7785794/94‑1 (MQ=60)
                                                                                        TCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACC       >  SRR4302545.9296961/1‑94 (MQ=60)
                                                                                         CTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCG      <  SRR4302545.10864016/94‑1 (MQ=60)
                                                                                          TGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGA     <  SRR4302545.515189/94‑1 (MQ=60)
                                                                                          TGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGA     <  SRR4302545.3791194/94‑1 (MQ=60)
                                                                                          TGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGA     <  SRR4302545.6258596/94‑1 (MQ=60)
                                                                                           GCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAA    <  SRR4302545.9124825/94‑1 (MQ=60)
                                                                                           GCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAA    >  SRR4302545.10510441/1‑94 (MQ=60)
                                                                                           GCCTGGCAGTAAGGGTTAGACGGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCGCCGAA    <  SRR4302545.5902602/94‑1 (MQ=60)
                                                                                            CCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAG   <  SRR4302545.3312286/94‑1 (MQ=60)
                                                                                            CCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAG   >  SRR4302545.9815011/1‑94 (MQ=60)
                                                                                             CTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGA  <  SRR4302545.278445/94‑1 (MQ=60)
                                                                                             CTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGA  >  SRR4302545.5606029/1‑94 (MQ=60)
                                                                                             CTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGA  >  SRR4302545.5019100/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGA  >  NC_000913/3325408‑3325594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑