Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,325,501	T→C	L24P (CTT→CCT)	yhbY →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,325,501	0	T	C	99.2%	926.3 / NA	264	L24P (CTT→CCT)	yhbY	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (141/120); minor base G (0/2); total (142/122)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

AACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCG  >  NC_000913/3325412‑3325590

aaCGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTgg                                                                                        >  2:4138628/1‑93 (MQ=255)

 aCGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGc                                                                                       <  2:3016802/93‑1 (MQ=255)

 aCGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGc                                                                                       >  2:3914199/1‑93 (MQ=255)

   gTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCAGCTCAAGCCAGTTGTTCTGCCTGGCAg                                                                                     >  2:3380341/1‑93 (MQ=255)

     aaGCATAAAATACGAAGAATCTGCGTACTAAACAAAACCCGCACCTGACAGGTCTGGCACATCCGCTCAAGCAAGTTGTTCTGCCTGGCAGTa                                                                                   <  2:6478784/93‑1 (MQ=255)

     aaGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTa                                                                                   <  1:2889159/93‑1 (MQ=255)

        cAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATg                                                                                <  1:2426580/93‑1 (MQ=255)

          aaaaaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGt                                                                              <  1:1497685/93‑1 (MQ=255)

           aaaaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGtt                                                                             >  1:4895354/1‑93 (MQ=255)

           aaaaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGtt                                                                             <  1:2281585/93‑1 (MQ=255)

            aaaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGttt                                                                            >  2:675896/1‑93 (MQ=255)

             aaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTg                                                                           >  2:1642270/1‑93 (MQ=255)

             aaTACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTg                                                                           >  2:620879/1‑93 (MQ=255)

                 cGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCg                                                                       >  1:4432408/1‑93 (MQ=255)

                  gATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGa                                                                      <  1:6113270/93‑1 (MQ=255)

                   aTGAATCTGAGTACTAAACAAAAACAGCGCCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGaa                                                                     <  1:995671/93‑1 (MQ=255)

                   aTGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGaa                                                                     <  1:2030054/93‑1 (MQ=255)

                   aTGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGaa                                                                     >  2:6263501/1‑93 (MQ=255)

                   aTGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGaa                                                                     >  2:4243046/1‑93 (MQ=255)

                      aaTCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAggg                                                                  <  2:6340725/93‑1 (MQ=255)

                      aaTCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAggg                                                                  >  2:2715618/1‑93 (MQ=255)

                       aTCTGAGTACTAAACATAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAgggg                                                                 <  2:3659233/93‑1 (MQ=255)

                       aTCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAgggt                                                                 >  1:2941019/1‑92 (MQ=255)

                       aTCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAgggg                                                                 <  1:696249/93‑1 (MQ=255)

                        tCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGt                                                                >  1:4211758/1‑93 (MQ=255)

                        tCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGt                                                                <  2:3095680/93‑1 (MQ=255)

                        tCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGt                                                                <  2:2294547/93‑1 (MQ=255)

                         cTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTg                                                               <  2:475988/93‑1 (MQ=255)

                          tGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGc                                                              <  2:5314299/93‑1 (MQ=255)

                          tGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGc                                                              <  1:172166/93‑1 (MQ=255)

                          tGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGc                                                              <  1:3609419/93‑1 (MQ=255)

                            aGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTg                                                            >  1:2644173/1‑93 (MQ=255)

                              tACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGTCCGAAGGGGTGCTGGc                                                          >  1:5875884/1‑93 (MQ=255)

                              tACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGc                                                          >  2:3995728/1‑93 (MQ=255)

                              tACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGc                                                          >  2:48256/1‑93 (MQ=255)

                              tACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGc                                                          <  1:4631107/93‑1 (MQ=255)

                                 tAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCga                                                       <  2:6113581/93‑1 (MQ=255)

                                 tAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCga                                                       >  2:2499742/1‑93 (MQ=255)

                                 tAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCga                                                       >  1:5569372/1‑93 (MQ=255)

                                   aaCAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCgaga                                                     <  1:1044227/93‑1 (MQ=255)

                                   aaCAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCgaga                                                     >  1:2797640/1‑93 (MQ=255)

                                    aCAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGAt                                                    <  2:225014/93‑1 (MQ=255)

                                       aaaaCAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGa                                                 >  1:5272574/1‑93 (MQ=255)

                                       aaaaCAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGa                                                 >  2:2572602/1‑93 (MQ=255)

                                       aaaaCAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGa                                                 >  2:5354048/1‑93 (MQ=255)

                                         aaCAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAAc                                               >  1:1365748/1‑93 (MQ=255)

                                           cAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACaa                                             <  1:4985264/93‑1 (MQ=255)

                                            aGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAg                                            <  1:5717222/93‑1 (MQ=255)

                                            aGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAg                                            <  1:3695572/93‑1 (MQ=255)

                                             gCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGc                                           <  1:3811138/93‑1 (MQ=255)

                                              cACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCg                                          <  2:4622560/93‑1 (MQ=255)

                                               aCCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGt                                         <  1:1016223/93‑1 (MQ=255)

                                               aCCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGt                                         >  1:185977/1‑93 (MQ=255)

                                               aCCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGt                                         >  1:3935624/1‑93 (MQ=255)

                                                ccTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGtt                                        >  2:2784908/1‑93 (MQ=255)

                                                 cTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTa                                       <  2:1314432/93‑1 (MQ=255)

                                                 cTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTa                                       >  2:4442596/1‑93 (MQ=255)

                                                   gAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTaga                                     >  2:3772858/1‑93 (MQ=255)

                                                    aaaGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTagag                                    <  2:1211865/93‑1 (MQ=255)

                                                    aaaGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTagag                                    >  2:75962/1‑93 (MQ=255)

                                                      aGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGTTGGCCGAGATTGAACAAGCGTTAGAGCa                                  >  2:1864001/1‑93 (MQ=255)

                                                       ggTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCAc                                 <  2:2423590/93‑1 (MQ=255)

                                                       ggTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCAc                                 >  2:247983/1‑93 (MQ=255)

                                                        gTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCAcc                                <  1:5930498/93‑1 (MQ=255)

                                                        gTCGGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCAcc                                <  2:3335375/93‑1 (MQ=255)

                                                         tCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCa                               <  1:5645954/93‑1 (MQ=255)

                                                          cTGGCCCACCCGCACAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGACCAAGCGTTAGAGCACCAt                              <  2:845356/93‑1 (MQ=255)

                                                           tGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATg                             >  2:4689007/1‑93 (MQ=255)

                                                           tGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATg                             >  2:1520938/1‑93 (MQ=255)

                                                           tGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATg                             >  2:5619823/1‑93 (MQ=255)

                                                           tGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGAGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATg                             >  2:5125670/1‑93 (MQ=255)

                                                            ggCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGa                            <  1:3230856/93‑1 (MQ=255)

                                                               ccaTCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACt                         <  1:398038/92‑1 (MQ=255)

                                                               acaTCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACt                         <  1:2139228/93‑1 (MQ=255)

                                                               acaTCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACt                         >  1:902692/1‑93 (MQ=255)

                                                               acaTCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACt                         >  1:5767742/1‑93 (MQ=255)

                                                                caTCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtc                        <  2:5948157/93‑1 (MQ=255)

                                                                  tCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcat                      <  1:3081642/93‑1 (MQ=255)

                                                                  tCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcat                      >  2:3583438/1‑93 (MQ=255)

                                                                   ccGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACtcatc                     <  1:1196382/93‑1 (MQ=255)

                                                                     gCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCaa                   <  1:1291698/93‑1 (MQ=255)

                                                                     gCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCaa                   >  1:3798604/1‑93 (MQ=255)

                                                                     gCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCaa                   >  2:3472697/1‑93 (MQ=255)

                                                                      cTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAg                  >  1:3538821/1‑93 (MQ=255)

                                                                       tCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAgg                 <  2:3199245/93‑1 (MQ=255)

                                                                       tCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAgg                 <  1:755216/93‑1 (MQ=255)

                                                                        cAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGt                <  1:255472/93‑1 (MQ=255)

                                                                        cAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGt                >  1:1584795/1‑93 (MQ=255)

                                                                         aaGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTg               <  2:546949/93‑1 (MQ=255)

                                                                         aaGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTg               <  1:2601154/93‑1 (MQ=255)

                                                                         aaGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTg               <  2:2000909/93‑1 (MQ=255)

                                                                           gCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGaa             >  2:4982270/1‑93 (MQ=255)

                                                                            ccAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGAACCATGAACTCATCAAGGTGaaa            >  2:4328023/1‑93 (MQ=255)

                                                                             cAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGaaaa           <  2:2032423/93‑1 (MQ=255)

                                                                              aGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAt          >  1:4055097/1‑93 (MQ=255)

                                                                               gttgttCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATc         <  1:1883121/93‑1 (MQ=255)

                                                                                  gttCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGcc      <  2:4815305/93‑1 (MQ=255)

                                                                                  gttCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGcc      >  2:5538014/1‑93 (MQ=255)

                                                                                    tCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCAc    <  1:163235/93‑1 (MQ=255)

                                                                                    tCTGCCTGCCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCCCCAc    <  2:2875095/93‑1 (MQ=255)

AACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCG  >  NC_000913/3325412‑3325590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGA  >  NC_000913/3325409‑3325594

CCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCT                                                                                              >  SRR4302537.6163757/1‑94 (MQ=60)

CCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCT                                                                                              <  SRR4302537.5984327/94‑1 (MQ=60)

 CCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTG                                                                                             <  SRR4302537.2417092/94‑1 (MQ=60)

   AACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGC                                                                                           >  SRR4302537.5644623/1‑94 (MQ=60)

     CGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAATCAGCACCTGAAATGTCTGGCACATCCTCTCAAGCCAGTTGTTCTGCCTGGCAG                                                                                         <  SRR4302537.2794599/94‑1 (MQ=60)

     CGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAG                                                                                         >  SRR4302537.5808066/1‑94 (MQ=60)

        AAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAA                                                                                      <  SRR4302537.2909435/94‑1 (MQ=60)

          GCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATG                                                                                    <  SRR4302537.4081462/94‑1 (MQ=60)

          GCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATG                                                                                    <  SRR4302537.847788/94‑1 (MQ=60)

          GCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATG                                                                                    >  SRR4302537.5884338/1‑94 (MQ=60)

          GCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATG                                                                                    <  SRR4302537.1524370/94‑1 (MQ=60)

           CAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGG                                                                                   >  SRR4302537.2283022/1‑94 (MQ=60)

           CAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGG                                                                                   <  SRR4302537.2443678/94‑1 (MQ=60)

           CAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGG                                                                                   >  SRR4302537.2530577/1‑94 (MQ=60)

            AAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGT                                                                                  >  SRR4302537.3584111/1‑94 (MQ=60)

             AGAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTT                                                                                 >  SRR4302537.6136230/1‑94 (MQ=60)

             AAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTT                                                                                 <  SRR4302537.1508136/94‑1 (MQ=60)

             AAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTT                                                                                 >  SRR4302537.4929156/1‑94 (MQ=60)

              AAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTT                                                                                <  SRR4302537.2297686/94‑1 (MQ=60)

               AAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTG                                                                               >  SRR4302537.121681/1‑94 (MQ=60)

                AATACGATGCATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGA                                                                              >  SRR4302537.5777022/1‑94 (MQ=60)

                  TACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACC                                                                            >  SRR4302537.5971883/1‑94 (MQ=60)

                  TACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACC                                                                            <  SRR4302537.3983846/94‑1 (MQ=60)

                   ACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCG                                                                           >  SRR4302537.4463016/1‑94 (MQ=60)

                     GATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAA                                                                         <  SRR4302537.6155282/94‑1 (MQ=60)

                     GATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAA                                                                         >  SRR4302537.911131/1‑94 (MQ=60)

                      ATGAATCTGAGTACTAAACAAAAACAGCGCCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAG                                                                        <  SRR4302537.1002550/94‑1 (MQ=60)

                      ATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGCAG                                                                        >  SRR4302537.153052/1‑94 (MQ=60)

                      ATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAG                                                                        <  SRR4302537.6394232/94‑1 (MQ=60)

                      ATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAG                                                                        <  SRR4302537.2044206/94‑1 (MQ=60)

                       TGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGG                                                                       <  SRR4302537.1168006/94‑1 (MQ=60)

                         AATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGT                                                                     >  SRR4302537.2961666/1‑94 (MQ=60)

                         AATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGG                                                                     <  SRR4302537.2633469/94‑1 (MQ=60)

                          ATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGT                                                                    >  SRR4302537.4241132/1‑94 (MQ=60)

                          ATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGT                                                                    <  SRR4302537.701021/94‑1 (MQ=60)

                           TCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTG                                                                   <  SRR4302537.2681815/94‑1 (MQ=60)

                            CTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGC                                                                  >  SRR4302537.5247271/1‑94 (MQ=60)

                            CTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGC                                                                  >  SRR4302537.5716302/1‑94 (MQ=60)

                             TGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCT                                                                 <  SRR4302537.3634863/94‑1 (MQ=60)

                             TGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCT                                                                 >  SRR4302537.1154348/1‑94 (MQ=60)

                             TGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCT                                                                 <  SRR4302537.173390/94‑1 (MQ=60)

                             TGAGTACTAAACAAAAACAGAACCTGAAAGGTCTGTCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCT                                                                 <  SRR4302537.1003149/94‑1 (MQ=60)

                              GAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTG                                                                >  SRR4302537.2662668/1‑94 (MQ=60)

                               AGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGG                                                               >  SRR4302537.4382171/1‑94 (MQ=60)

                                GTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGTCCGAAGGGGTGCTGGC                                                              >  SRR4302537.5916196/1‑94 (MQ=60)

                                GTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGC                                                              >  SRR4302537.2260872/1‑94 (MQ=60)

                                GTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGC                                                              >  SRR4302537.4194920/1‑94 (MQ=60)

                                 TACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCC                                                             <  SRR4302537.3837815/94‑1 (MQ=60)

                                 TACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCC                                                             <  SRR4302537.4663145/94‑1 (MQ=60)

                                  ACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCG                                                            >  SRR4302537.5959953/1‑94 (MQ=60)

                                  ACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCG                                                            >  SRR4302537.706790/1‑94 (MQ=60)

                                   CTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGA                                                           >  SRR4302537.1882489/1‑94 (MQ=60)

                                   CTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGA                                                           >  SRR4302537.2990091/1‑94 (MQ=60)

                                   CTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGA                                                           >  SRR4302537.5607439/1‑94 (MQ=60)

                                    TAAACAAAAACAGCGCCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAG                                                          <  SRR4302537.3371823/94‑1 (MQ=60)

                                    TAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAG                                                          <  SRR4302537.2921189/94‑1 (MQ=60)

                                     AAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGA                                                         >  SRR4302537.2817202/1‑94 (MQ=60)

                                     AAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGA                                                         >  SRR4302537.1672809/1‑94 (MQ=60)

                                      AACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGAT                                                        >  SRR4302537.5340246/1‑94 (MQ=60)

                                      AACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGAT                                                        <  SRR4302537.1051433/94‑1 (MQ=60)

                                       ACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATT                                                       >  SRR4302537.2613666/1‑94 (MQ=60)

                                       ACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATT                                                       >  SRR4302537.4724838/1‑94 (MQ=60)

                                       ACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATT                                                       >  SRR4302537.4016149/1‑94 (MQ=60)

                                        CAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTG                                                      >  SRR4302537.3115909/1‑94 (MQ=60)

                                         AAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGA                                                     >  SRR4302537.5308860/1‑94 (MQ=60)

                                           AAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAAC                                                   >  SRR4302537.1375264/1‑94 (MQ=60)

                                            AACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACA                                                  <  SRR4302537.2882152/94‑1 (MQ=60)

                                              CAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAG                                                <  SRR4302537.4560431/94‑1 (MQ=60)

                                              CAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAG                                                <  SRR4302537.5019626/94‑1 (MQ=60)

                                              CAGCACCTGAAAGGTCTGGCACAGCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAG                                                <  SRR4302537.977653/94‑1 (MQ=60)

                                               AGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGC                                               <  SRR4302537.3721580/94‑1 (MQ=60)

                                               AGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGC                                               <  SRR4302537.5756351/94‑1 (MQ=60)

                                                GCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCG                                              <  SRR4302537.1918882/94‑1 (MQ=60)

                                                GCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCG                                              <  SRR4302537.3837857/94‑1 (MQ=60)

                                                 CACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGT                                             >  SRR4302537.187299/1‑94 (MQ=60)

                                                 CACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGT                                             >  SRR4302537.3963132/1‑94 (MQ=60)

                                                 CACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGT                                             >  SRR4302537.1759816/1‑94 (MQ=60)

                                                  ACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTT                                            <  SRR4302537.1023222/94‑1 (MQ=60)

                                                  ACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTT                                            >  SRR4302537.4712233/1‑94 (MQ=60)

                                                     TGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGA                                         <  SRR4302537.1752570/94‑1 (MQ=60)

                                                     TGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGA                                         >  SRR4302537.311496/1‑94 (MQ=60)

                                                     TGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGA                                         >  SRR4302537.4476968/1‑94 (MQ=60)

                                                     TGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGGGCTGGCCGAGATTGAACAAGCGTTAGA                                         >  SRR4302537.1400255/1‑94 (MQ=60)

                                                      GAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAG                                        <  SRR4302537.1883701/94‑1 (MQ=60)

                                                        AAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCA                                      >  SRR4302537.1040778/1‑94 (MQ=60)

                                                         AGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCAC                                     <  SRR4302537.5767277/94‑1 (MQ=60)

                                                           GTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCA                                   <  SRR4302537.5971188/94‑1 (MQ=60)

                                                            TCTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCAT                                  <  SRR4302537.5684597/94‑1 (MQ=60)

                                                             CTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATG                                 >  SRR4302537.807625/1‑94 (MQ=60)

                                                             CTGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATG                                 <  SRR4302537.211270/94‑1 (MQ=60)

                                                              TGGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGA                                >  SRR4302537.5123449/1‑94 (MQ=60)

                                                               GGCACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAA                               <  SRR4302537.3253624/94‑1 (MQ=60)

                                                                GCACAGCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAAC                              <  SRR4302537.494621/94‑1 (MQ=60)

                                                                 CACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACT                             <  SRR4302537.6402097/94‑1 (MQ=60)

                                                                 CACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACT                             >  SRR4302537.5807272/1‑94 (MQ=60)

                                                                 CACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACT                             >  SRR4302537.908925/1‑94 (MQ=60)

                                                                  CCATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTC                            <  SRR4302537.400797/94‑1 (MQ=60)

                                                                  ACATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTC                            <  SRR4302537.2154287/94‑1 (MQ=60)

                                                                    ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          >  SRR4302537.3785081/1‑94 (MQ=60)

                                                                    ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          <  SRR4302537.3580751/94‑1 (MQ=60)

                                                                    ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          >  SRR4302537.2632206/1‑94 (MQ=60)

                                                                    ATCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCAT                          <  SRR4302537.1359084/94‑1 (MQ=60)

                                                                     TCCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATC                         <  SRR4302537.3103106/94‑1 (MQ=60)

                                                                      CCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCA                        >  SRR4302537.1603151/1‑94 (MQ=60)

                                                                      CCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCA                        >  SRR4302537.3768185/1‑94 (MQ=60)

                                                                      CCGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCA                        <  SRR4302537.1204689/94‑1 (MQ=60)

                                                                       CGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAA                       >  SRR4302537.2474814/1‑94 (MQ=60)

                                                                       CGCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAA                       >  SRR4302537.3825254/1‑94 (MQ=60)

                                                                        GCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAG                      >  SRR4302537.3563815/1‑94 (MQ=60)

                                                                        GCTCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAG                      <  SRR4302537.1300679/94‑1 (MQ=60)

                                                                          TCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGT                    <  SRR4302537.760367/94‑1 (MQ=60)

                                                                          TCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGT                    >  SRR4302537.5513980/1‑94 (MQ=60)

                                                                          TCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGT                    >  SRR4302537.1595873/1‑94 (MQ=60)

                                                                          TCAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGT                    >  SRR4302537.2378352/1‑94 (MQ=60)

                                                                           CAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTG                   <  SRR4302537.3828841/94‑1 (MQ=60)

                                                                           CAAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTG                   <  SRR4302537.257283/94‑1 (MQ=60)

                                                                            AAGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGA                  <  SRR4302537.2619341/94‑1 (MQ=60)

                                                                             AGCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAA                 <  SRR4302537.2863047/94‑1 (MQ=60)

                                                                              GCCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAA                >  SRR4302537.2588615/1‑94 (MQ=60)

                                                                               CCAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAA               >  SRR4302537.3530731/1‑94 (MQ=60)

                                                                                CAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAT              <  SRR4302537.1728532/94‑1 (MQ=60)

                                                                                CAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAT              >  SRR4302537.3987843/1‑94 (MQ=60)

                                                                                CAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAT              <  SRR4302537.5734187/94‑1 (MQ=60)

                                                                                CAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAAT              >  SRR4302537.4083440/1‑94 (MQ=60)

                                                                                CAGTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGAGAAAAT              >  SRR4302537.1466244/1‑94 (MQ=60)

                                                                                  GTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCG            >  SRR4302537.384167/1‑94 (MQ=60)

                                                                                  GTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCG            <  SRR4302537.1896215/94‑1 (MQ=60)

                                                                                  GTTGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCG            >  SRR4302537.4147223/1‑94 (MQ=60)

                                                                                    TGTTCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCC          <  SRR4302537.3662873/94‑1 (MQ=60)

                                                                                       TCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACC       <  SRR4302537.164399/94‑1 (MQ=60)

                                                                                       TCTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACC       <  SRR4302537.2941099/94‑1 (MQ=60)

                                                                                        CTGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGATCTCATCAAGGTGAAAATCGCCACCG      <  SRR4302537.1256168/94‑1 (MQ=60)

                                                                                         TGCCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGA     <  SRR4302537.2053868/94‑1 (MQ=60)

                                                                                           CCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAG   <  SRR4302537.124666/94‑1 (MQ=60)

                                                                                           CCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAG   <  SRR4302537.6152459/94‑1 (MQ=60)

                                                                                           CCTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAG   >  SRR4302537.4384749/1‑94 (MQ=60)

                                                                                            CTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGA  <  SRR4302537.5485629/94‑1 (MQ=60)

CCCAACGTAAGCAAAAAATACGATGAATCTGAGTACTAAACAAAAACAGCACCTGAAAGGTCTGGCACATCCGCTCAAGCCAGTTGTTCTGCTTGGCAGTAATGGTTTGACCGAAGGGGTGCTGGCCGAGATTGAACAAGCGTTAGAGCACCATGAACTCATCAAGGTGAAAATCGCCACCGAAGA  >  NC_000913/3325409‑3325594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`