Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,979,535 +G coding (1036/1386 nt) yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,979,5341.G99.7% 2271.9 / ‑3.0 644coding (1035/1386 nt)yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (366/275);  total (367/277)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 701 total aligned reads displayed.

GAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG  >  NC_000913/3979441‑3979623
                                                                                              |                                                                                         
gAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAtt                                                                                            <  1:180785/94‑1 (MQ=255)
 aaaaaCCGTCAGTTCCCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑g                                                                                          <  2:6301638/94‑1 (MQ=255)
 aaaaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑g                                                                                          <  1:9675937/94‑1 (MQ=255)
 aaaaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑g                                                                                          >  2:191303/1‑94 (MQ=255)
  aaaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑gg                                                                                         <  2:10002718/94‑2 (MQ=255)
  aaaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑gg                                                                                         <  2:7976644/94‑2 (MQ=255)
  aaaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑gg                                                                                         <  1:1610391/94‑2 (MQ=255)
   aaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑ggc                                                                                        <  2:3180491/94‑3 (MQ=255)
   aaaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑ggc                                                                                        <  1:291261/94‑3 (MQ=255)
    aaCCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑ggct                                                                                       >  1:4860796/1‑91 (MQ=255)
       cGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAt                                                                                     <  1:432520/94‑1 (MQ=255)
       cGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAt                                                                                     >  1:732027/1‑94 (MQ=255)
       cGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCAt                                                                                     <  2:7422701/94‑1 (MQ=255)
        gTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATg                                                                                    >  1:246781/1‑94 (MQ=255)
          cAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGcc                                                                                  >  1:7416215/1‑94 (MQ=255)
          cAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGcc                                                                                  >  2:8311826/1‑94 (MQ=255)
           aGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCt                                                                                 >  2:1464697/1‑94 (MQ=255)
           aGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCt                                                                                 >  2:10135019/1‑94 (MQ=255)
            gTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTg                                                                                <  2:2114434/94‑1 (MQ=255)
            gTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTg                                                                                <  1:3445472/94‑1 (MQ=255)
              tACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaa                                                                              >  1:4626065/1‑92 (MQ=255)
               aCCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaac                                                                             <  2:2263783/94‑4 (MQ=255)
               aCCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaac                                                                             <  2:4915444/94‑4 (MQ=255)
                 cggcggCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTa                                                                          >  1:55243/1‑94 (MQ=255)
                  ggcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAc                                                                         >  2:8958177/1‑94 (MQ=255)
                    cggcAATGGCGAACGTTTCCCGTCACGGCGTACCGGTTGCGGGGGTGGCCGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                       >  1:3567230/1‑94 (MQ=255)
                    cggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                       >  2:1367150/1‑94 (MQ=255)
                    cggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                       >  2:7080746/1‑94 (MQ=255)
                     ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatc                                                                      >  1:1282493/1‑94 (MQ=255)
                      gcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatca                                                                     >  2:9346371/1‑94 (MQ=255)
                       cAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                    <  1:6469490/94‑1 (MQ=255)
                        aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAtt                                                                   >  2:3494935/1‑94 (MQ=255)
                         aTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTc                                                                  >  2:4668443/1‑94 (MQ=255)
                            gCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                               <  2:3656262/94‑1 (MQ=255)
                            gCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                               <  1:3866552/94‑1 (MQ=255)
                              gggggTTTCCCGGCACGGCGTACCGGTTGCGCGTGTGGCAGTATCTATTGCGATTCTGCTAATTGGCTCGTGCCT‑GAACTACATCATTCCCAAt                                                             <  1:786671/90‑1 (MQ=255)
                              gAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAt                                                             <  1:8747401/94‑1 (MQ=255)
                               aaaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                            <  2:617288/94‑1 (MQ=255)
                                  gTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGc                                                         >  1:6051799/1‑94 (MQ=255)
                                     tCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGc                                                      >  1:10352192/1‑94 (MQ=255)
                                       ccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgt                                                    >  1:395793/1‑94 (MQ=255)
                                        cGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtg                                                   <  2:7246553/94‑1 (MQ=255)
                                          tCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtg                                                 <  2:2701483/94‑1 (MQ=255)
                                           cACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                                <  1:5638753/94‑1 (MQ=255)
                                             cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGttt                                              >  2:7615916/1‑94 (MQ=255)
                                              ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTg                                             >  2:3073959/1‑94 (MQ=255)
                                              ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTg                                             <  1:9162508/94‑1 (MQ=255)
                                               gCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgt                                            <  2:8709112/94‑1 (MQ=255)
                                                cGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtt                                           >  2:1157873/1‑93 (MQ=255)
                                                cGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                           <  1:928947/94‑1 (MQ=255)
                                                 gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                          <  1:8605518/94‑1 (MQ=255)
                                                 gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                          >  2:507802/1‑94 (MQ=255)
                                                   aCCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                        >  2:9715967/1‑94 (MQ=255)
                                                     cGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgt                                      >  2:4487621/1‑94 (MQ=255)
                                                      ggTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                     >  2:6432805/1‑94 (MQ=255)
                                                       gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑TAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtct                                    >  2:590743/1‑94 (MQ=255)
                                                       gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtct                                    <  2:4006698/94‑1 (MQ=255)
                                                        ttGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtcta                                   <  1:5346230/94‑1 (MQ=255)
                                                          gCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACa                                 <  1:956557/94‑1 (MQ=255)
                                                           cGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                >  1:10605423/1‑94 (MQ=255)
                                                           cGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                >  1:145316/1‑94 (MQ=255)
                                                            gtgtgtGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGt                               <  1:2619995/92‑1 (MQ=255)
                                                            gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGt                               >  2:6701437/1‑94 (MQ=255)
                                                              gtgtGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGc                             <  2:8042576/94‑1 (MQ=255)
                                                               tgtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                            <  2:4094636/94‑1 (MQ=255)
                                                               tgtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                            <  2:948414/94‑1 (MQ=255)
                                                                gtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                           <  1:7673107/94‑1 (MQ=255)
                                                                 tgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAg                          <  1:6221442/94‑1 (MQ=255)
                                                                    cAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGt                       >  1:7006186/1‑94 (MQ=255)
                                                                      gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGc                     >  1:1375650/1‑94 (MQ=255)
                                                                      gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGc                     >  2:8511236/1‑94 (MQ=255)
                                                                      gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGc                     >  1:842896/1‑94 (MQ=255)
                                                                       tATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GCACTACATCATTCCCAATCCGGAGCGTGTGTTTGGCTACGTCTACAGTGCCAGCGTGCt                    >  2:10509671/1‑94 (MQ=255)
                                                                       tATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                    <  1:10059988/94‑1 (MQ=255)
                                                                       tATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                    >  2:8814210/1‑94 (MQ=255)
                                                                        aTCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCtt                   <  2:2908981/94‑1 (MQ=255)
                                                                         tCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                  >  1:10455161/1‑94 (MQ=255)
                                                                         tCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                  >  2:3899299/1‑94 (MQ=255)
                                                                         tCTATTGCTATTCTGCTAATTGGCTCATACCT‑GTACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                  >  2:257473/1‑94 (MQ=255)
                                                                          cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                 >  2:4337557/1‑94 (MQ=255)
                                                                          cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                 <  1:1025/94‑1 (MQ=255)
                                                                          cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                 <  1:2790147/94‑1 (MQ=255)
                                                                          cTATTGCTATTCTGCTAATTGGCTCACGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                 >  1:3325062/1‑94 (MQ=255)
                                                                           tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                <  1:5169935/94‑1 (MQ=255)
                                                                           tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                >  1:9785100/1‑94 (MQ=255)
                                                                           tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                <  1:8031439/94‑1 (MQ=255)
                                                                           tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                >  2:2450286/1‑94 (MQ=255)
                                                                           tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                >  2:3446130/1‑94 (MQ=255)
                                                                            aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg               >  1:3097526/1‑94 (MQ=255)
                                                                                cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAt           <  1:2026153/94‑1 (MQ=255)
                                                                                cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAt           <  2:5313398/94‑1 (MQ=255)
                                                                                 tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg          >  2:5560252/1‑94 (MQ=255)
                                                                                 tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg          <  1:4122133/94‑1 (MQ=255)
                                                                                  aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg         >  1:6662995/1‑94 (MQ=255)
                                                                                  aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg         >  1:7499757/1‑94 (MQ=255)
                                                                                   ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATggc        <  1:8937783/94‑2 (MQ=255)
                                                                                       gCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGTAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCa    <  1:6442435/94‑1 (MQ=255)
                                                                                         tAATTGGCTCATGCCT‑GACCTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATg  <  2:920296/94‑1 (MQ=255)
                                                                                         tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATg  >  1:2298376/1‑94 (MQ=255)
                                                                                         tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATg  >  1:5893351/1‑94 (MQ=255)
                                                                                              |                                                                                         
GAAAAACCGTCAGTTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG  >  NC_000913/3979441‑3979623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 381 total aligned reads displayed.
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979454‑3979639
                                                                                             |                                                                                             
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTGA                                                                                               >  SRR4302545.9474232/1‑94 (MQ=60)
 TACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTGAA                                                                                              >  SRR4302545.4744340/1‑94 (MQ=60)
 TACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTGAA                                                                                              <  SRR4302545.1009032/94‑1 (MQ=60)
  ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAAC                                                                                            <  SRR4302545.9286317/94‑1 (MQ=60)
  ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGAGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAAC                                                                                            <  SRR4302545.4043069/94‑1 (MQ=60)
   CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                           >  SRR4302545.878979/1‑94 (MQ=60)
   CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                           >  SRR4302545.1166194/1‑94 (MQ=60)
    CGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTA                                                                                          >  SRR4302545.8769163/1‑94 (MQ=60)
     GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAC                                                                                         <  SRR4302545.6764628/94‑1 (MQ=60)
     GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAC                                                                                         >  SRR4302545.2298153/1‑94 (MQ=60)
      GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACA                                                                                        <  SRR4302545.7698812/94‑1 (MQ=60)
      GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACA                                                                                        >  SRR4302545.4044531/1‑94 (MQ=60)
       CGGCAATGGCGAACGTTTCCCGTCACGGCGTACCGGTTGCGGGGGTGGCCGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                       >  SRR4302545.3654393/1‑94 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                      <  SRR4302545.106149/94‑1 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                      >  SRR4302545.5684650/1‑94 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                      <  SRR4302545.1387684/94‑1 (MQ=60)
         GCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCA                                                                                     >  SRR4302545.5484622/1‑94 (MQ=60)
         GCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCA                                                                                     <  SRR4302545.1704720/94‑1 (MQ=60)
         GCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCA                                                                                     <  SRR4302545.7628168/94‑1 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAT                                                                                    <  SRR4302545.5008029/94‑1 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGCGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATTCCT‑GAACTACATCAT                                                                                    >  SRR4302545.2627175/1‑94 (MQ=60)
           AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATT                                                                                   <  SRR4302545.6513097/94‑1 (MQ=60)
             TGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCC                                                                                 <  SRR4302545.932919/94‑1 (MQ=60)
              GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCC                                                                                >  SRR4302545.2659503/1‑94 (MQ=60)
              GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCC                                                                                <  SRR4302545.399071/94‑1 (MQ=60)
               GCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCA                                                                               <  SRR4302545.6327499/94‑1 (MQ=60)
               GCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCA                                                                               <  SRR4302545.3963068/94‑1 (MQ=60)
               GCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCA                                                                               >  SRR4302545.4809797/1‑94 (MQ=60)
               GCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCA                                                                               >  SRR4302545.3006642/1‑94 (MQ=60)
                CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAA                                                                              >  SRR4302545.10515536/1‑94 (MQ=60)
                CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAA                                                                              >  SRR4302545.3577479/1‑94 (MQ=60)
                CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAA                                                                              <  SRR4302545.1900957/94‑1 (MQ=60)
                 ggggGTTTCCCGGCACGGCGTACCGGTTGCGCGTGTGGCAGTATCTATTGCGATTCTGCTAATTGGCTCGTGCCT‑GAACTACATCATTCCCAAT                                                                             <  SRR4302545.804367/90‑1 (MQ=60)
                 GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             >  SRR4302545.8900109/1‑94 (MQ=60)
                 GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             <  SRR4302545.5975599/94‑1 (MQ=60)
                 GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             <  SRR4302545.9001321/94‑1 (MQ=60)
                 GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                             <  SRR4302545.1456442/94‑1 (MQ=60)
                  AAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATC                                                                            <  SRR4302545.1180891/94‑1 (MQ=60)
                  AAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATC                                                                            <  SRR4302545.4740805/94‑1 (MQ=60)
                   AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCC                                                                           <  SRR4302545.4306597/94‑1 (MQ=60)
                   AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCC                                                                           <  SRR4302545.2117993/94‑1 (MQ=60)
                    AGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCG                                                                          <  SRR4302545.273662/94‑1 (MQ=60)
                    AGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCG                                                                          >  SRR4302545.989082/1‑94 (MQ=60)
                     GTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGC                                                                         >  SRR4302545.6215797/1‑94 (MQ=60)
                      TTTCCCGTCACGGCGTACCGGTTGCGGGGGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCA                                                                        >  SRR4302545.5642613/1‑94 (MQ=60)
                       TTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                                       >  SRR4302545.10864339/1‑94 (MQ=60)
                        TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      <  SRR4302545.7331414/94‑1 (MQ=60)
                        TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      >  SRR4302545.10657323/1‑94 (MQ=60)
                        TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      <  SRR4302545.5543443/94‑1 (MQ=60)
                         CCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCG                                                                     >  SRR4302545.94789/1‑94 (MQ=60)
                         CCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCG                                                                     <  SRR4302545.10439241/94‑1 (MQ=60)
                          CCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGT                                                                    >  SRR4302545.404602/1‑94 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   >  SRR4302545.10236216/1‑94 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   <  SRR4302545.9375856/94‑1 (MQ=60)
                            GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                                  <  SRR4302545.10121388/94‑1 (MQ=60)
                              CACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGT                                                                >  SRR4302545.1518963/1‑94 (MQ=60)
                              CACGGCGTACCGGTTGCGGGTGTGGCAGCATCTATTGCTATTCTGCTCATTGGCTCATGCCT‑GAACTACATCCTTCCCAATCCGCAGCGTGcct                                                                >  SRR4302545.991281/1‑91 (MQ=60)
                                CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              >  SRR4302545.6820844/1‑94 (MQ=60)
                                CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              >  SRR4302545.636794/1‑94 (MQ=60)
                                CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              >  SRR4302545.8987158/1‑94 (MQ=60)
                                CGGCGTACCGGTGGCGTGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATG‑CTTGAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302545.5878135/94‑1 (MQ=60)
                                 GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             >  SRR4302545.5240683/1‑94 (MQ=60)
                                 GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             <  SRR4302545.375648/94‑1 (MQ=60)
                                 GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             <  SRR4302545.9431420/94‑1 (MQ=60)
                                 GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             >  SRR4302545.173459/1‑94 (MQ=60)
                                   CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                           <  SRR4302545.4153101/94‑1 (MQ=60)
                                   CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                           >  SRR4302545.2438520/1‑94 (MQ=60)
                                     TAGCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                                         <  SRR4302545.2257019/94‑1 (MQ=60)
                                     TACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                                         >  SRR4302545.5748703/1‑94 (MQ=60)
                                     TACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                                         <  SRR4302545.6603632/94‑1 (MQ=60)
                                     TACCGGTGGCGGTTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAGCTACATCATTCCCAATCCTCAGCGCGCGTTTGTCTA                                                         <  SRR4302545.1454826/94‑1 (MQ=53)
                                      ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        >  SRR4302545.6640329/1‑94 (MQ=60)
                                       CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                       >  SRR4302545.1989496/1‑94 (MQ=60)
                                       CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                       <  SRR4302545.10706773/94‑1 (MQ=60)
                                        CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                      <  SRR4302545.3134765/94‑1 (MQ=60)
                                        CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                      >  SRR4302545.2571266/1‑94 (MQ=60)
                                         GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                     >  SRR4302545.10062595/1‑94 (MQ=60)
                                          GTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCT                                                    >  SRR4302545.1562078/1‑94 (MQ=60)
                                          GTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCT                                                    <  SRR4302545.924016/94‑1 (MQ=60)
                                            TGGGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCCTTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  <  SRR4302545.8151782/94‑1 (MQ=60)
                                            TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  <  SRR4302545.5404459/94‑1 (MQ=60)
                                            TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  >  SRR4302545.8067329/1‑94 (MQ=60)
                                            TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  >  SRR4302545.659603/1‑94 (MQ=60)
                                             GCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACA                                                 >  SRR4302545.8904070/1‑94 (MQ=60)
                                              CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                <  SRR4302545.247275/94‑1 (MQ=60)
                                              CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                >  SRR4302545.7819834/1‑94 (MQ=60)
                                              CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                >  SRR4302545.10917680/1‑94 (MQ=60)
                                               cttccgatctctgatctTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               >  SRR4302545.3376113/18‑94 (MQ=60)
                                               GTGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302545.2683088/94‑1 (MQ=60)
                                               GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302545.8270380/94‑1 (MQ=60)
                                               GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302545.6083742/94‑1 (MQ=60)
                                               GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302545.1765206/94‑1 (MQ=60)
                                                GGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTG                                              >  SRR4302545.688580/1‑94 (MQ=60)
                                                GGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTG                                              >  SRR4302545.6049204/1‑94 (MQ=60)
                                                 GTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGGGTTTGTCTACGTCTcacgtgc                                             >  SRR4302545.8287625/1‑87 (MQ=60)
                                                  TGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCC                                            >  SRR4302545.5057273/1‑94 (MQ=60)
                                                   GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           <  SRR4302545.7890330/94‑1 (MQ=60)
                                                   GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           >  SRR4302545.6714416/1‑94 (MQ=60)
                                                   GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           >  SRR4302545.9955850/1‑94 (MQ=60)
                                                    TGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                          <  SRR4302545.2991235/94‑1 (MQ=60)
                                                     GGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGC                                         >  SRR4302545.2910051/1‑94 (MQ=60)
                                                      GCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCG                                        <  SRR4302545.10059705/94‑1 (MQ=60)
                                                       CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       <  SRR4302545.8545497/94‑1 (MQ=60)
                                                       CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       >  SRR4302545.7199493/1‑94 (MQ=60)
                                                        AGTTTCTATTGCTATTCGGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTATAGTGCCAGCGTG                                      >  SRR4302545.9665382/1‑94 (MQ=60)
                                                         GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302545.3886121/1‑94 (MQ=60)
                                                         GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302545.4663253/1‑94 (MQ=60)
                                                         GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302545.1406471/1‑94 (MQ=60)
                                                         GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302545.861800/1‑94 (MQ=60)
                                                         GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGCTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302545.350082/1‑94 (MQ=60)
                                                          TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302545.6258394/1‑94 (MQ=60)
                                                          TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302545.5703757/1‑94 (MQ=60)
                                                          TATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302545.4903187/1‑94 (MQ=60)
                                                          TATCTATTCCGATTCTGCTAATTGGCTCATGGCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTGTGTCTACGTCTACAGTGCCAGCGTTCT                                    <  SRR4302545.8996579/94‑1 (MQ=60)
                                                           ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                   >  SRR4302545.7118574/1‑94 (MQ=60)
                                                           ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                   >  SRR4302545.9864706/1‑94 (MQ=60)
                                                           ATCTATTGCTATTCTGCTAATTGGCTCATGCAT‑GAAATACATCATTCCCAATCCGCAGCGTGTGTTTGTCGACGTCTACAGTACCAGCGTGCTT                                   <  SRR4302545.10873411/94‑1 (MQ=60)
                                                            TCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTC                                  >  SRR4302545.1057218/1‑94 (MQ=60)
                                                            TCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTC                                  >  SRR4302545.10763392/1‑94 (MQ=60)
                                                             CTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 >  SRR4302545.2659962/1‑94 (MQ=60)
                                                             CTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 <  SRR4302545.6644012/94‑1 (MQ=60)
                                                             CTATTGCTATTCTGCTAATTGGCTCACGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 >  SRR4302545.6935166/1‑94 (MQ=60)
                                                              TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                >  SRR4302545.3313062/1‑94 (MQ=60)
                                                              TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                <  SRR4302545.5302940/94‑1 (MQ=60)
                                                              TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                >  SRR4302545.3570128/1‑94 (MQ=60)
                                                              TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                >  SRR4302545.5460817/1‑94 (MQ=60)
                                                               ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               <  SRR4302545.2422401/94‑1 (MQ=60)
                                                               ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               >  SRR4302545.1474257/1‑94 (MQ=60)
                                                               ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               >  SRR4302545.3173822/1‑94 (MQ=60)
                                                               ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               <  SRR4302545.2933034/94‑1 (MQ=60)
                                                               ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCCG                               >  SRR4302545.7621955/1‑94 (MQ=60)
                                                                TTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              >  SRR4302545.7336043/1‑94 (MQ=60)
                                                                TTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              <  SRR4302545.4438160/94‑1 (MQ=60)
                                                                TTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGCGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              >  SRR4302545.9631846/1‑94 (MQ=60)
                                                                 TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                             >  SRR4302545.3444355/1‑94 (MQ=60)
                                                                 TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                             <  SRR4302545.4235225/94‑1 (MQ=60)
                                                                  TCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            <  SRR4302545.1331411/94‑1 (MQ=60)
                                                                  GCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGttgg                            >  SRR4302545.2955091/1‑90 (MQ=60)
                                                                  GCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            >  SRR4302545.3193975/1‑94 (MQ=60)
                                                                  GCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            >  SRR4302545.9101833/1‑94 (MQ=60)
                                                                  GCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            <  SRR4302545.1118942/94‑1 (MQ=60)
                                                                   CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           <  SRR4302545.2076150/94‑1 (MQ=60)
                                                                    TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          <  SRR4302545.3453873/94‑1 (MQ=60)
                                                                    TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302545.8203520/1‑94 (MQ=60)
                                                                    TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          <  SRR4302545.2838251/94‑1 (MQ=60)
                                                                    TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302545.10700117/1‑94 (MQ=60)
                                                                    TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          <  SRR4302545.4225258/94‑1 (MQ=60)
                                                                    TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          <  SRR4302545.9980176/94‑1 (MQ=60)
                                                                     ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302545.7711989/1‑94 (MQ=60)
                                                                     ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         <  SRR4302545.7934183/94‑1 (MQ=60)
                                                                     ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302545.7572711/1‑94 (MQ=60)
                                                                     ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         <  SRR4302545.4198163/94‑1 (MQ=60)
                                                                     ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302545.3843182/1‑94 (MQ=60)
                                                                     ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302545.6845682/1‑94 (MQ=60)
                                                                      TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        <  SRR4302545.5334975/94‑1 (MQ=60)
                                                                      TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        <  SRR4302545.10477796/94‑1 (MQ=60)
                                                                      TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        <  SRR4302545.6167711/94‑1 (MQ=60)
                                                                      TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                        <  SRR4302545.1852878/94‑1 (MQ=60)
                                                                      TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGC                        <  SRR4302545.9197685/94‑1 (MQ=60)
                                                                       TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG                       >  SRR4302545.10563391/1‑94 (MQ=60)
                                                                        CTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGCTGGTGC                      >  SRR4302545.8333841/1‑94 (MQ=60)
                                                                         TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGCTGGTGCC                     >  SRR4302545.6028863/1‑94 (MQ=60)
                                                                         TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     >  SRR4302545.1300588/1‑94 (MQ=60)
                                                                          GCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCA                    <  SRR4302545.7739651/94‑1 (MQ=60)
                                                                           CTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   >  SRR4302545.7648354/1‑94 (MQ=60)
                                                                           CTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   >  SRR4302545.3322367/1‑94 (MQ=60)
                                                                            TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  >  SRR4302545.10365232/1‑94 (MQ=60)
                                                                            TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  >  SRR4302545.6052206/1‑94 (MQ=60)
                                                                            TAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  >  SRR4302545.2354353/1‑94 (MQ=60)
                                                                              ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302545.4956773/1‑94 (MQ=60)
                                                                              ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302545.1005790/1‑94 (MQ=60)
                                                                              ATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302545.8863363/1‑94 (MQ=60)
                                                                               TG‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTT              >  SRR4302545.2612746/1‑94 (MQ=60)
                                                                               TG‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTT              >  SRR4302545.1311127/1‑94 (MQ=60)
                                                                                G‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             >  SRR4302545.8010287/1‑94 (MQ=60)
                                                                                G‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGAGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             >  SRR4302545.9763166/1‑94 (MQ=60)
                                                                                   CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG           >  SRR4302545.2757240/1‑94 (MQ=60)
                                                                                   CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG           >  SRR4302545.8376966/1‑94 (MQ=60)
                                                                                   CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGCGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG           >  SRR4302545.2723547/1‑94 (MQ=60)
                                                                                   CTCATGCAT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG           <  SRR4302545.7281104/94‑1 (MQ=60)
                                                                                    ctcctgcatGAACTGCATCAGTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGGGCCATGGTTTGTG           <  SRR4302545.8596016/85‑1 (MQ=60)
                                                                                    TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA          <  SRR4302545.2114069/94‑1 (MQ=60)
                                                                                    TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA          <  SRR4302545.8726673/94‑1 (MQ=60)
                                                                                      ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATT        >  SRR4302545.3472070/1‑94 (MQ=60)
                                                                                      ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA        >  SRR4302545.6431003/1‑94 (MQ=60)
                                                                                      ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA        >  SRR4302545.3415367/1‑94 (MQ=60)
                                                                                      ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA        <  SRR4302545.1698340/94‑1 (MQ=60)
                                                                                      ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA        <  SRR4302545.5664501/94‑1 (MQ=60)
                                                                                      ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCACGGTTTGTGATA        >  SRR4302545.6431002/1‑94 (MQ=60)
                                                                                       atgcatGAACTTCATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTTTACAGTGTCAGCGGGCTTTCGGGGAGGGTGCCATGGTTTGTGATA        <  SRR4302545.8685310/88‑1 (MQ=60)
                                                                                       TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT       >  SRR4302545.1489527/1‑94 (MQ=60)
                                                                                        GCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATT      >  SRR4302545.563262/1‑94 (MQ=60)
                                                                                         CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTA     >  SRR4302545.7974546/1‑94 (MQ=60)
                                                                                           ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    <  SRR4302545.7453054/92‑1 (MQ=60)
                                                                                           ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGCTTGTGATATTGT    >  SRR4302545.9016513/3‑94 (MQ=60)
                                                                                            TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   >  SRR4302545.8116762/1‑94 (MQ=60)
                                                                                            TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   <  SRR4302545.9797005/94‑1 (MQ=60)
                                                                                            TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   <  SRR4302545.8647181/94‑1 (MQ=60)
                                                                                            TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGACAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGca   >  SRR4302545.5571589/1‑92 (MQ=60)
                                                                                             GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  <  SRR4302545.6901835/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979454‑3979639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑