Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,979,546 Δ1 bp coding (1047/1386 nt) yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,979,5450G.100.0% 999.8 / NA 212coding (1046/1386 nt)yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (114/98);  total (114/98)

Only 100 of 219 total aligned reads displayed.

TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979454‑3979633
                                                                                            |                                                                                        
 tACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTga                                                                                         <  2:631548/93‑2 (MQ=255)
     ggcggcAATGGCGAAAGTTTCCCGTCACGGCGTATCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTa                                                                                    <  1:5693754/93‑1 (MQ=255)
      gcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAc                                                                                   <  2:1034612/93‑1 (MQ=255)
      gcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAc                                                                                   <  1:5887924/93‑1 (MQ=255)
       cggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAca                                                                                  <  2:6459078/93‑1 (MQ=255)
        ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                                 <  2:1988420/93‑1 (MQ=255)
         gcAATGGCGAAATTTTCCCGTCCCGCCGTACCGGTTGCGGTTGT‑GCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatca                                                                               <  2:5805759/93‑1 (MQ=255)
         gcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatc                                                                                >  1:3415792/1‑93 (MQ=255)
          cAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatca                                                                               <  2:4875956/93‑1 (MQ=255)
           aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                              <  1:3833404/93‑1 (MQ=255)
           aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                              <  2:1510341/93‑1 (MQ=255)
           aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                              >  2:2480365/1‑93 (MQ=255)
           aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                              <  1:2855006/93‑1 (MQ=255)
           aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                              >  1:2386626/1‑93 (MQ=255)
            aTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAtt                                                                             >  2:5641490/1‑93 (MQ=255)
            aTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGACAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAtt                                                                             >  1:3466186/1‑93 (MQ=255)
              ggCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTcc                                                                           >  1:1579781/1‑93 (MQ=255)
              ggCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTcc                                                                           <  2:4693025/93‑1 (MQ=255)
                cGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                                         >  1:3409701/1‑93 (MQ=255)
                 gAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCaa                                                                        >  1:6187782/1‑93 (MQ=255)
                  aaaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAt                                                                       >  2:4246097/1‑93 (MQ=255)
                   aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  2:3675509/93‑1 (MQ=255)
                   aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  2:4334621/93‑1 (MQ=255)
                      tttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGc                                                                   >  2:3856682/1‑93 (MQ=255)
                       ttCGCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCa                                                                  <  1:1069897/93‑1 (MQ=255)
                       ttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCa                                                                  <  1:5884335/93‑1 (MQ=255)
                       ttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCa                                                                  <  2:4570287/93‑1 (MQ=255)
                            gTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtg                                                             >  1:4130868/1‑93 (MQ=255)
                                cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                                         <  1:2016845/93‑1 (MQ=255)
                                cGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGtt                                                         <  2:732459/93‑1 (MQ=255)
                                 ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGttt                                                        <  1:6240801/93‑1 (MQ=255)
                                  gCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTg                                                       >  1:2161856/1‑93 (MQ=255)
                                    gTACCGGTTGCGGGTGTGGCGGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     <  1:489194/93‑1 (MQ=255)
                                    gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     >  1:2699660/1‑93 (MQ=255)
                                     tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                                    >  2:2821671/1‑93 (MQ=255)
                                     tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                                    >  1:1352041/1‑93 (MQ=255)
                                     tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                                    >  2:1257621/1‑93 (MQ=255)
                                       ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                                  <  2:1812691/93‑1 (MQ=255)
                                          gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               >  1:3329213/1‑93 (MQ=255)
                                           ttGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtct                                              <  2:4965354/93‑1 (MQ=255)
                                           ttGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtct                                              <  2:18559/93‑1 (MQ=255)
                                            tGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtcta                                             <  1:5673650/93‑1 (MQ=255)
                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          >  2:6400929/1‑93 (MQ=255)
                                                ggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGt                                         <  2:3220218/93‑1 (MQ=255)
                                                ggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGt                                         >  1:5198014/1‑93 (MQ=255)
                                                 gtgtGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTg                                        >  2:1442931/1‑93 (MQ=255)
                                                 gtgtGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTg                                        >  2:2939967/1‑93 (MQ=255)
                                                 gtgtGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTg                                        >  1:394464/1‑93 (MQ=255)
                                                   gtgGCCGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                                      >  2:4092056/1‑93 (MQ=255)
                                                   gtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                                      >  2:1603791/1‑93 (MQ=255)
                                                       cAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCg                                  <  1:3631934/93‑1 (MQ=255)
                                                        aGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGt                                 <  2:2342405/93‑1 (MQ=255)
                                                        aGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGt                                 >  1:1168771/1‑93 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  1:6154404/1‑93 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  1:4339487/1‑93 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAATTGGCGCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  1:6463906/1‑93 (MQ=255)
                                                          tATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGc                               >  2:5357706/1‑93 (MQ=255)
                                                           aTCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              >  2:6446435/1‑93 (MQ=255)
                                                            tCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCtt                             >  2:5318804/1‑93 (MQ=255)
                                                              tATTGCTATTCTGCTAATTGGCTCATTCCT‑GAACTACATCATTCCCAATCCGCAGCGCGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                           >  1:2804077/1‑93 (MQ=255)
                                                              tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                           <  1:1125560/93‑1 (MQ=255)
                                                               aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                          >  1:1741318/1‑93 (MQ=255)
                                                                ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         <  2:2383396/93‑1 (MQ=255)
                                                                ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         >  1:2214811/1‑93 (MQ=255)
                                                                ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         <  2:5941131/93‑1 (MQ=255)
                                                                   cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      >  1:1371998/1‑93 (MQ=255)
                                                                   cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      >  1:277580/1‑93 (MQ=255)
                                                                   cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      >  1:2513589/1‑93 (MQ=255)
                                                                   cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      <  1:1771895/93‑1 (MQ=255)
                                                                   cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      <  2:4152296/93‑1 (MQ=255)
                                                                    tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGag                     >  2:6128464/1‑92 (MQ=255)
                                                                    tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAt                     >  1:2273986/1‑93 (MQ=255)
                                                                     aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg                    >  1:6023827/1‑93 (MQ=255)
                                                                     aTTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATg                    >  1:6130847/1‑93 (MQ=255)
                                                                      ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   >  1:4454831/1‑93 (MQ=255)
                                                                      ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   >  1:4278189/1‑93 (MQ=255)
                                                                      ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   <  2:4201801/93‑1 (MQ=255)
                                                                         tGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                >  2:5430767/1‑93 (MQ=255)
                                                                         tGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                >  1:3772920/1‑93 (MQ=255)
                                                                         tGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                >  2:3283802/1‑93 (MQ=255)
                                                                           cTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCa              >  1:3271308/1‑93 (MQ=255)
                                                                           cTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCa              <  2:712650/93‑1 (MQ=255)
                                                                            tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             <  1:2208480/93‑1 (MQ=255)
                                                                            tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             >  1:3491709/1‑93 (MQ=255)
                                                                              attggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGCTGGTGCCATgg           >  1:1240256/5‑93 (MQ=255)
                                                                              attggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATgg           >  2:5103299/5‑93 (MQ=255)
                                                                               ttggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGt          >  1:4664499/4‑93 (MQ=255)
                                                                               ttggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGt          >  2:5188138/4‑93 (MQ=255)
                                                                                 ggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        <  1:912543/92‑1 (MQ=255)
                                                                                 ggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        >  2:3391295/2‑93 (MQ=255)
                                                                                 ggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        <  1:1457109/92‑1 (MQ=255)
                                                                                 ggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        <  1:697009/92‑1 (MQ=255)
                                                                                  gCTCATGCCT‑GAACTTCATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtg       <  2:870170/93‑1 (MQ=255)
                                                                                     cATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGa    <  2:5487822/93‑1 (MQ=255)
                                                                                     cATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGa    >  1:4815935/1‑93 (MQ=255)
                                                                                      ctGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   <  2:3420940/92‑1 (MQ=255)
                                                                                      aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   <  1:4241409/93‑1 (MQ=255)
                                                                                      aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   >  2:2912391/1‑93 (MQ=255)
                                                                                       tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGata  >  2:3775832/1‑93 (MQ=255)
                                                                                       tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGata  <  1:302346/93‑1 (MQ=255)
                                                                                            |                                                                                        
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979454‑3979633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑T‑G‑CTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979454‑3979639
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TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATCG‑G‑CTCATGCCTGA                                                                                               <  SRR4302537.3512417/94‑1 (MQ=60)
  ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAAC                                                                                            <  SRR4302537.2270598/94‑1 (MQ=60)
  ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAAC                                                                                            <  SRR4302537.4358925/94‑1 (MQ=60)
    CGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTA                                                                                          >  SRR4302537.97271/1‑94 (MQ=60)
     GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTATCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTAC                                                                                         <  SRR4302537.5732694/94‑1 (MQ=60)
     GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTAC                                                                                         <  SRR4302537.4748275/94‑1 (MQ=60)
      GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACA                                                                                        >  SRR4302537.5731382/1‑94 (MQ=60)
      GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACA                                                                                        <  SRR4302537.3769073/94‑1 (MQ=60)
      GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACA                                                                                        <  SRR4302537.5928325/94‑1 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATC                                                                                      >  SRR4302537.3439977/1‑94 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATC                                                                                      >  SRR4302537.2885362/1‑94 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATC                                                                                      <  SRR4302537.1624702/94‑1 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCAT                                                                                    >  SRR4302537.2403435/1‑94 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCAT                                                                                    >  SRR4302537.5068113/1‑94 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCAT                                                                                    >  SRR4302537.2156560/1‑94 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCAT                                                                                    <  SRR4302537.3049210/94‑1 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCAT                                                                                    <  SRR4302537.3649829/94‑1 (MQ=60)
           AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATT                                                                                   <  SRR4302537.3860270/94‑1 (MQ=60)
           AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATT                                                                                   <  SRR4302537.2875020/94‑1 (MQ=60)
           AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGACAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATT                                                                                   >  SRR4302537.3490698/1‑94 (MQ=60)
           AATGGCGAAAGTTTCCCGCCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATT                                                                                   <  SRR4302537.989982/94‑1 (MQ=60)
             TGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCC                                                                                 >  SRR4302537.1590820/1‑94 (MQ=60)
              GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCC                                                                                <  SRR4302537.6225359/94‑1 (MQ=60)
              GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCC                                                                                <  SRR4302537.4230555/94‑1 (MQ=60)
              GGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCC                                                                                <  SRR4302537.5925103/94‑1 (MQ=60)
               GCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCA                                                                               >  SRR4302537.3433854/1‑94 (MQ=60)
                CGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAA                                                                              >  SRR4302537.6230407/1‑94 (MQ=60)
                 GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAAT                                                                             >  SRR4302537.1154919/1‑94 (MQ=60)
                  AAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATC                                                                            <  SRR4302537.3801484/94‑1 (MQ=60)
                   AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCC                                                                           <  SRR4302537.6516886/94‑1 (MQ=60)
                    AGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCG                                                                          <  SRR4302537.1564568/94‑1 (MQ=60)
                      TTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCA                                                                        >  SRR4302537.5789051/1‑94 (MQ=60)
                       TTCGCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                                       <  SRR4302537.1077276/94‑1 (MQ=60)
                       TTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                                       <  SRR4302537.5924710/94‑1 (MQ=60)
                          CCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGT                                                                    <  SRR4302537.1376736/94‑1 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   >  SRR4302537.6193231/1‑94 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   >  SRR4302537.4159697/1‑94 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   <  SRR4302537.5657590/94‑1 (MQ=60)
                            GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                                  >  SRR4302537.2538994/1‑94 (MQ=60)
                                CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302537.2202946/94‑1 (MQ=60)
                                CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302537.2030905/94‑1 (MQ=60)
                                CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302537.3504168/94‑1 (MQ=60)
                                 GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             >  SRR4302537.2831284/1‑94 (MQ=60)
                                 GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             >  SRR4302537.2177094/1‑94 (MQ=60)
                                 GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                             <  SRR4302537.6283703/94‑1 (MQ=60)
                                  GCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGT                                                            <  SRR4302537.5863804/94‑1 (MQ=60)
                                   CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                           >  SRR4302537.2718519/1‑94 (MQ=60)
                                    GTACCGGTTGCGGGTGTGGCGGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302537.492554/94‑1 (MQ=60)
                                    GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          >  SRR4302537.1361461/1‑94 (MQ=60)
                                    GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          >  SRR4302537.3434631/1‑94 (MQ=60)
                                    GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          >  SRR4302537.6344554/1‑94 (MQ=60)
                                    GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TCG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302537.1962982/94‑1 (MQ=60)
                                      ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        >  SRR4302537.239353/1‑94 (MQ=60)
                                         GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                     >  SRR4302537.3352770/1‑94 (MQ=60)
                                         GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                     >  SRR4302537.6273322/1‑94 (MQ=60)
                                          GTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCT                                                    >  SRR4302537.4456331/1‑94 (MQ=60)
                                            TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  <  SRR4302537.5712470/94‑1 (MQ=60)
                                              CGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                >  SRR4302537.2041274/1‑94 (MQ=60)
                                               GGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               >  SRR4302537.5957791/1‑94 (MQ=60)
                                               GGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               >  SRR4302537.5233775/1‑94 (MQ=60)
                                                GGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTG                                              >  SRR4302537.397198/1‑94 (MQ=60)
                                                GGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTG                                              >  SRR4302537.6217232/1‑94 (MQ=60)
                                                 GTGTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGC                                             >  SRR4302537.3464766/1‑94 (MQ=60)
                                                   GTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           <  SRR4302537.1085766/94‑1 (MQ=60)
                                                   GTGGCAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           <  SRR4302537.2813350/94‑1 (MQ=60)
                                                       CAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       <  SRR4302537.3657524/94‑1 (MQ=60)
                                                       CAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       >  SRR4302537.1291814/1‑94 (MQ=60)
                                                       CAGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       >  SRR4302537.1176902/1‑94 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302537.854320/1‑94 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302537.4369599/1‑94 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302537.6196745/1‑94 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      <  SRR4302537.4897154/94‑1 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      <  SRR4302537.2225267/94‑1 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAAT‑TGG‑CGCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302537.6507695/1‑94 (MQ=60)
                                                         GTATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302537.3335255/1‑94 (MQ=60)
                                                          TATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    >  SRR4302537.1457583/1‑94 (MQ=60)
                                                          TATCTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCT                                    <  SRR4302537.2499344/94‑1 (MQ=60)
                                                             CTATTGCTATTCTGCTAAT‑TGG‑CTCATTCCT‑GAACTACATCATTCCCAATCCGCAGCGCGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 >  SRR4302537.2823675/1‑94 (MQ=60)
                                                             CTATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 <  SRR4302537.4273310/94‑1 (MQ=60)
                                                              TATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                >  SRR4302537.1753455/1‑94 (MQ=60)
                                                              TATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                <  SRR4302537.1133365/94‑1 (MQ=60)
                                                               ATTGCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               >  SRR4302537.2230431/1‑94 (MQ=60)
                                                                  TCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            <  SRR4302537.2470087/94‑1 (MQ=60)
                                                                  GCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            >  SRR4302537.1381576/1‑94 (MQ=60)
                                                                  GCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            >  SRR4302537.279521/1‑94 (MQ=60)
                                                                  GCTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                            >  SRR4302537.2531259/1‑94 (MQ=60)
                                                                   CTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           >  SRR4302537.4507613/1‑94 (MQ=60)
                                                                   CTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           >  SRR4302537.2290044/1‑94 (MQ=60)
                                                                   CTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           <  SRR4302537.6153472/94‑1 (MQ=60)
                                                                   CTATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           <  SRR4302537.1784238/94‑1 (MQ=60)
                                                                    TATTCTGCTAAT‑TGG‑CTCATGCCT‑GCACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302537.3370306/1‑94 (MQ=60)
                                                                    TATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302537.1242884/1‑94 (MQ=60)
                                                                    TATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302537.6172987/1‑94 (MQ=60)
                                                                    TATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          >  SRR4302537.6065220/1‑94 (MQ=60)
                                                                     ATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302537.4485596/1‑94 (MQ=60)
                                                                     ATTCTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                         >  SRR4302537.4307924/1‑94 (MQ=60)
                                                                        CTGCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGC                      >  SRR4302537.3799406/1‑94 (MQ=60)
                                                                          GCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCA                    >  SRR4302537.4504205/1‑94 (MQ=60)
                                                                          GCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCA                    >  SRR4302537.3294396/1‑94 (MQ=60)
                                                                          GCTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCA                    >  SRR4302537.4052086/1‑94 (MQ=60)
                                                                           CTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   >  SRR4302537.1778798/1‑94 (MQ=60)
                                                                           CTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   >  SRR4302537.3516408/1‑94 (MQ=60)
                                                                           CTAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   >  SRR4302537.3887759/1‑94 (MQ=60)
                                                                           CTAAT‑T‑GTCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   <  SRR4302537.2775006/94‑1 (MQ=60)
                                                                            TAAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATG                  <  SRR4302537.2224048/94‑1 (MQ=60)
                                                                             AAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGCTGGTGCCATGG                 >  SRR4302537.1248878/1‑94 (MQ=60)
                                                                             AAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                 <  SRR4302537.4345542/94‑1 (MQ=60)
                                                                             AAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                 <  SRR4302537.6257414/94‑1 (MQ=60)
                                                                             AAT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                 <  SRR4302537.2286347/94‑1 (MQ=60)
                                                                              AT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302537.1397115/1‑94 (MQ=60)
                                                                              AT‑TGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302537.4696738/1‑94 (MQ=60)
                                                                                 G‑G‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             <  SRR4302537.1467259/94‑1 (MQ=60)
                                                                                 G‑G‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             <  SRR4302537.918838/94‑1 (MQ=60)
                                                                                 G‑G‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             <  SRR4302537.701784/94‑1 (MQ=60)
                                                                                     CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG           >  SRR4302537.4054868/1‑94 (MQ=60)
                                                                                      TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA          >  SRR4302537.4849175/1‑94 (MQ=60)
                                                                                       CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         >  SRR4302537.3253720/1‑94 (MQ=60)
                                                                                        ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA        <  SRR4302537.4270897/94‑1 (MQ=60)
                                                                                         TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT       <  SRR4302537.304450/94‑1 (MQ=60)
                                                                                           CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG     <  SRR4302537.5966116/94‑1 (MQ=60)
                                                                                           CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG     <  SRR4302537.2674442/94‑1 (MQ=60)
                                                                                           CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG     >  SRR4302537.5451832/1‑94 (MQ=60)
                                                                                             ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    >  SRR4302537.4737564/3‑94 (MQ=60)
                                                                                             ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    >  SRR4302537.3074444/3‑94 (MQ=60)
                                                                                             ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGGTTGTGATATTGA    >  SRR4302537.550038/3‑94 (MQ=60)
                                                                                              TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT   <  SRR4302537.6457861/94‑1 (MQ=60)
                                                                                               GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  <  SRR4302537.5113248/94‑1 (MQ=60)
                                                                                               GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  SRR4302537.4958823/1‑94 (MQ=60)
                                                                                               |                                                                                             
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAAT‑T‑G‑CTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979454‑3979639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑