Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,051,170 +C intergenic (+172/+279) hemN → / ← glnG O2‑independent coproporphyrinogen III oxidase/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,1701.C99.8% 1493.1 / ‑4.5 509intergenic (+172/+279)hemN/glnGO2‑independent coproporphyrinogen III oxidase/GlnG
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (265/243);  total (265/244)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 559 total aligned reads displayed.

CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACAC  >  NC_000913/4051076‑4051257
                                                                                               |                                                                                       
    gCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgctgg                                                                                      <  2:3171378/94‑5 (MQ=255)
    gCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgctgg                                                                                      >  2:10475708/1‑90 (MQ=255)
       acaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑GATCTGAGCATGCTGCCTGATGCGGCGCTGGTcg                                                                                   >  2:2231480/1‑94 (MQ=25)
       acaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg                                                                                   >  1:7112907/1‑94 (MQ=25)
       acaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg                                                                                   >  2:1568865/1‑94 (MQ=25)
         aaaaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcgcg                                                                                 <  1:2381347/94‑1 (MQ=25)
           aaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTc                                                                               <  1:6803237/94‑1 (MQ=25)
           aaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTc                                                                               >  1:8871668/1‑94 (MQ=21)
           aaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTc                                                                               >  1:4046528/1‑94 (MQ=21)
               tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAt                                                                           >  1:1653758/1‑94 (MQ=21)
                 ttGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCa                                                                         <  1:3614715/94‑1 (MQ=18)
                 ttGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCa                                                                         <  2:8459942/94‑1 (MQ=18)
                  tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg                                                                        <  2:8141766/94‑1 (MQ=21)
                  tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAg                                                                        <  1:2990777/94‑1 (MQ=18)
                   gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAgg                                                                       <  2:1189589/94‑1 (MQ=18)
                   gAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCATGATGCGGCGCTGGTCGCGTCTTATCAgg                                                                       <  1:8997908/94‑1 (MQ=18)
                    aaaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGc                                                                      >  2:6201031/1‑94 (MQ=18)
                     aaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGcc                                                                     >  2:4047290/1‑94 (MQ=18)
                      attcttGCTTTTGTAGGCCGGATAAGGCGTTTACGTCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGcccc                                                                    >  1:2102114/1‑93 (MQ=17)
                       ttattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                                                                   <  1:7229738/94‑1 (MQ=18)
                        tattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCTTGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                                                                  <  1:7376910/94‑1 (MQ=17)
                        tattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                                                                  <  2:8679460/94‑1 (MQ=18)
                         attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACg                                                                 >  2:4985794/1‑94 (MQ=17)
                         attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCCCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACg                                                                 >  1:7762267/1‑94 (MQ=17)
                           tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAc                                                               <  2:5461438/94‑1 (MQ=18)
                           tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAc                                                               <  2:2544269/94‑1 (MQ=18)
                            gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt                                                              <  1:9164256/94‑1 (MQ=17)
                            gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt                                                              >  2:2917640/1‑94 (MQ=18)
                             cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACtt                                                             >  1:3456138/1‑94 (MQ=17)
                             cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACtt                                                             >  2:3826322/1‑94 (MQ=17)
                              ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACttt                                                            >  1:9526617/1‑94 (MQ=17)
                              ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACttt                                                            >  2:4704936/1‑94 (MQ=17)
                              ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACttt                                                            <  1:3860257/94‑1 (MQ=17)
                                ttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtct                                                          <  1:5703264/94‑1 (MQ=17)
                                 tGTAGGCCGGATAAGGCGTTTACGCCGCATAAGG‑CATCTGACGATGATGCCTGATGCGGCGCTGGTCGGGTCTTATCAGGCCTACGACTTtctc                                                         <  1:6200572/94‑1 (MQ=18)
                                   tAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTccc                                                       >  1:3140647/1‑93 (MQ=17)
                                   tAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg                                                       >  1:8149807/1‑94 (MQ=18)
                                   tAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg                                                       >  1:4529308/1‑94 (MQ=18)
                                    aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc                                                      >  2:2745956/1‑94 (MQ=18)
                                    aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc                                                      >  2:4232340/1‑94 (MQ=18)
                                     ggCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCa                                                     <  2:7991300/94‑1 (MQ=18)
                                      gCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt                                                    <  2:5618349/94‑1 (MQ=18)
                                       ccGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATc                                                   >  2:7225055/1‑94 (MQ=18)
                                       ccGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATc                                                   >  2:572578/1‑94 (MQ=18)
                                       ccGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATc                                                   >  2:7642752/1‑94 (MQ=18)
                                        cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc                                                  <  2:3707477/94‑1 (MQ=18)
                                        cGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcc                                                  >  1:6553473/1‑94 (MQ=18)
                                         ggATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCg                                                 >  2:6764345/1‑94 (MQ=18)
                                          gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg                                                >  2:6601469/1‑94 (MQ=18)
                                          gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg                                                <  1:6348465/94‑1 (MQ=18)
                                          gATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg                                                >  2:2417329/1‑94 (MQ=18)
                                          gATAAGGCAGTTACGCCGCAGCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg                                                <  1:8301930/94‑1 (MQ=18)
                                           aTAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc                                               <  2:9279/94‑1 (MQ=18)
                                           aTAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc                                               >  1:417736/1‑94 (MQ=21)
                                            tAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCa                                              >  2:7053436/1‑94 (MQ=21)
                                              aGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATa                                            <  1:5972987/94‑1 (MQ=21)
                                                gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaaa                                          >  1:7054987/1‑94 (MQ=21)
                                                gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaaa                                          >  1:5280027/1‑94 (MQ=21)
                                                 cGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaac                                         <  1:10353805/94‑1 (MQ=21)
                                                  gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaaca                                        <  2:4460428/94‑1 (MQ=21)
                                                    ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaac                                      >  1:10551147/1‑94 (MQ=25)
                                                     tACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACg                                     <  1:10257210/94‑1 (MQ=25)
                                                       cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAg                                   <  2:8985963/94‑1 (MQ=25)
                                                       cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAg                                   <  1:9322791/94‑1 (MQ=25)
                                                       cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAg                                   <  2:6399417/94‑1 (MQ=25)
                                                         ccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCt                                 <  2:1421747/94‑1 (MQ=25)
                                                           gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTc                               >  1:10027287/1‑94 (MQ=37)
                                                            cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa                              >  1:9867804/1‑94 (MQ=37)
                                                             aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAg                             <  2:7694896/94‑1 (MQ=37)
                                                              tCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGc                            <  2:10211300/94‑1 (MQ=37)
                                                                caggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCt                           >  2:5059135/5‑94 (MQ=38)
                                                                caggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCt                           <  1:10476058/90‑1 (MQ=37)
                                                                caggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCt                           >  2:8207901/5‑94 (MQ=38)
                                                                  ggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTaa                         <  2:5201696/92‑1 (MQ=255)
                                                                    cATCTGAGGATGATGCCTGATGCGGGGATGGTCGCGTCTTATCAGTCCTCCGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAc                       <  2:6875511/94‑1 (MQ=38)
                                                                    cATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAc                       <  2:9774137/94‑1 (MQ=255)
                                                                    cATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAc                       >  1:1499577/1‑94 (MQ=255)
                                                                    cATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAc                       >  1:197110/1‑94 (MQ=255)
                                                                    cATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAc                       <  2:3455347/94‑1 (MQ=255)
                                                                     aTCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa                      >  2:9625097/1‑94 (MQ=255)
                                                                     aTCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa                      <  2:9533652/94‑1 (MQ=255)
                                                                      tCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAg                     >  2:10569595/1‑94 (MQ=255)
                                                                      tCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAg                     <  1:1751800/94‑1 (MQ=255)
                                                                         gAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGccc                  >  1:2590541/1‑94 (MQ=255)
                                                                         gAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGccc                  <  1:4944688/94‑1 (MQ=255)
                                                                          aGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCa                 >  1:4445195/1‑94 (MQ=255)
                                                                            cATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAAt               <  2:7393567/94‑1 (MQ=255)
                                                                            cATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAAt               <  1:1263926/94‑1 (MQ=255)
                                                                            cATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAAt               <  1:8608297/94‑1 (MQ=255)
                                                                            cATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAAt               >  2:1929665/1‑94 (MQ=255)
                                                                                atgCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAtt           >  1:8049850/1‑94 (MQ=255)
                                                                                atgCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAtt           <  2:1709617/94‑1 (MQ=255)
                                                                                   ccTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCg        <  2:9168958/94‑1 (MQ=255)
                                                                                    cTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTTAGCTAAACAGCCCAATCATTGCgg       >  1:7588835/1‑94 (MQ=255)
                                                                                       aTGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGcac    >  1:5096445/1‑94 (MQ=255)
                                                                                       aTGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGcac    <  1:5537972/94‑1 (MQ=255)
                                                                                        tGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGcaca   >  1:3348560/1‑94 (MQ=255)
                                                                                        tGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGcaca   >  1:2760541/1‑94 (MQ=255)
                                                                                        tGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGcaca   <  2:6037563/94‑1 (MQ=255)
                                                                                         gcggcgcTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGcacac  <  2:2660486/94‑1 (MQ=255)
                                                                                               |                                                                                       
CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACAC  >  NC_000913/4051076‑4051257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 365 total aligned reads displayed.
TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CA‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTT  >  NC_000913/4057666‑4057852
                                                                                               |                                                                                             
taccggcagGACTCTGAAACAAGCTCGGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CA‑C                                                                                               <  SRR4302545.6303534/85‑1 (MQ=60)
TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CA‑C                                                                                               <  SRR4302545.443286/94‑1 (MQ=60)
 ATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CA‑CC                                                                                              >  SRR4302545.3372066/1‑94 (MQ=60)
 ATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CA‑CC                                                                                              >  SRR4302545.10871101/1‑94 (MQ=60)
 ATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATAGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CA‑CC                                                                                              >  SRR4302545.2275735/1‑94 (MQ=60)
   CCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CA‑Cctg                                                                                            <  SRR4302545.9786409/94‑4 (MQ=60)
    CGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGA                                                                                            >  SRR4302545.7083671/1‑94 (MQ=60)
    CGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGA                                                                                            >  SRR4302545.10521300/1‑94 (MQ=60)
    CGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGA                                                                                            >  SRR4302545.8540536/1‑94 (MQ=60)
     GCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGAC                                                                                           >  SRR4302545.931686/1‑94 (MQ=60)
     GCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGAC                                                                                           >  SRR4302545.821405/1‑94 (MQ=60)
     GCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGAC                                                                                           >  SRR4302545.7334896/1‑94 (MQ=60)
      CATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACG                                                                                          >  SRR4302545.3534083/1‑94 (MQ=60)
      CATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACG                                                                                          >  SRR4302545.6278596/1‑94 (MQ=60)
       ATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGG                                                                                         >  SRR4302545.2719368/1‑94 (MQ=60)
       ATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGG                                                                                         <  SRR4302545.6273543/94‑1 (MQ=60)
        TGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGA                                                                                        >  SRR4302545.7034211/1‑94 (MQ=60)
        TGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGA                                                                                        >  SRR4302545.7112754/1‑94 (MQ=60)
         GACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGACCTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAA                                                                                       <  SRR4302545.4554811/94‑1 (MQ=60)
         GACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGCCTACGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAA                                                                                       >  SRR4302545.10360188/1‑94 (MQ=60)
           CTCTGGAACAAGCTCTGGAGTTCATCGATGAAGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAA                                                                                     <  SRR4302545.8929779/94‑1 (MQ=60)
            TCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAAC                                                                                    <  SRR4302545.8612454/94‑1 (MQ=60)
            TCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAAC                                                                                    <  SRR4302545.3458334/94‑1 (MQ=60)
             CTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACG                                                                                   <  SRR4302545.4265756/94‑1 (MQ=60)
              TGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAGAACGA                                                                                  >  SRR4302545.9889648/1‑94 (MQ=60)
               GGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGAT                                                                                 >  SRR4302545.2908762/1‑94 (MQ=60)
               GGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGAT                                                                                 <  SRR4302545.4006354/94‑1 (MQ=60)
                GAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATC                                                                                >  SRR4302545.7244285/1‑94 (MQ=60)
                GAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATC                                                                                <  SRR4302545.8966105/94‑1 (MQ=60)
                GAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATC                                                                                >  SRR4302545.4809358/1‑94 (MQ=60)
                 AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCG                                                                               <  SRR4302545.4399067/94‑1 (MQ=60)
                 AACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCG                                                                               <  SRR4302545.10850331/94‑1 (MQ=60)
                  ACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGT                                                                              >  SRR4302545.8761391/1‑94 (MQ=60)
                  ACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGT                                                                              >  SRR4302545.9704479/1‑94 (MQ=60)
                   CAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTC                                                                             >  SRR4302545.5043134/1‑94 (MQ=60)
                   CAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTC                                                                             >  SRR4302545.3358465/1‑94 (MQ=60)
                    AAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCG                                                                            >  SRR4302545.8150534/1‑94 (MQ=60)
                    AAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCG                                                                            <  SRR4302545.3591814/94‑1 (MQ=60)
                    AAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCG                                                                            <  SRR4302545.1349131/94‑1 (MQ=60)
                    AAGCGCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTCACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCG                                                                            <  SRR4302545.10624897/94‑1 (MQ=60)
                     AGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGC                                                                           >  SRR4302545.9119371/1‑94 (MQ=60)
                     AGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGC                                                                           <  SRR4302545.8656357/94‑1 (MQ=60)
                      GCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCC                                                                          <  SRR4302545.2752334/94‑1 (MQ=60)
                      GCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCC                                                                          >  SRR4302545.2781571/1‑94 (MQ=60)
                       CTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCG                                                                         >  SRR4302545.4762962/1‑94 (MQ=60)
                       CTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCG                                                                         <  SRR4302545.6144169/94‑1 (MQ=60)
                        cttggtgtgaatctATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCTTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGC                                                                        <  SRR4302545.2712624/80‑1 (MQ=60)
                        TCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGCTCGTCGCacc                                                                        >  SRR4302545.2798973/1‑91 (MQ=60)
                        TCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCAGC                                                                        >  SRR4302545.3313881/1‑94 (MQ=60)
                        TCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCATAAACGT‑CACCTGACGGAAAACGATCGTCGCCGC                                                                        >  SRR4302545.3313883/1‑94 (MQ=60)
                         CTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCG                                                                       >  SRR4302545.9135420/1‑94 (MQ=60)
                          TGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGC                                                                      >  SRR4302545.3818017/1‑94 (MQ=60)
                          TGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGC                                                                      >  SRR4302545.3814678/1‑94 (MQ=60)
                            GAGTTCATCGAAGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCA                                                                    <  SRR4302545.4060001/94‑1 (MQ=60)
                              GTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAAC                                                                  <  SRR4302545.3323961/94‑1 (MQ=60)
                              GTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAAC                                                                  >  SRR4302545.8385568/1‑94 (MQ=60)
                               TTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACC                                                                 >  SRR4302545.5418159/1‑94 (MQ=60)
                               TTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACC                                                                 >  SRR4302545.9946140/1‑94 (MQ=60)
                               TTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACC                                                                 <  SRR4302545.2613332/94‑1 (MQ=60)
                               TTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACC                                                                 >  SRR4302545.7478960/1‑94 (MQ=60)
                                TCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCG                                                                >  SRR4302545.9016835/1‑94 (MQ=60)
                                  ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCG                                                              >  SRR4302545.10261794/1‑94 (MQ=60)
                                  ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCG                                                              >  SRR4302545.1726817/1‑94 (MQ=60)
                                   TCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGC                                                             >  SRR4302545.987144/1‑94 (MQ=60)
                                   TCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGC                                                             >  SRR4302545.6612862/1‑94 (MQ=60)
                                    CGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCA                                                            >  SRR4302545.3841153/1‑94 (MQ=60)
                                    CGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCA                                                            >  SRR4302545.7380634/1‑94 (MQ=60)
                                    CGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCA                                                            >  SRR4302545.6610880/1‑94 (MQ=60)
                                     GATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAC                                                           <  SRR4302545.3801049/94‑1 (MQ=60)
                                     GATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGACCCAACCGCGCAC                                                           >  SRR4302545.7285/1‑94 (MQ=60)
                                     GATGAGGACGAACTGGTAGAAGTGACTCCGTCCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCAC                                                           <  SRR4302545.4655955/94‑1 (MQ=60)
                                      ATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACC                                                          <  SRR4302545.1983504/94‑1 (MQ=60)
                                      ATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACC                                                          <  SRR4302545.2671324/94‑1 (MQ=60)
                                       TGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCG                                                         <  SRR4302545.3155397/94‑1 (MQ=60)
                                       TGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCG                                                         <  SRR4302545.10163069/94‑1 (MQ=60)
                                        GATGACGAACTGGTAGAAGTGACTCCGACCTATATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGA                                                        <  SRR4302545.10199328/94‑1 (MQ=60)
                                         ATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAA                                                       <  SRR4302545.8980557/94‑1 (MQ=60)
                                          TGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGCAA                                                      >  SRR4302545.6087488/1‑94 (MQ=60)
                                          TGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAA                                                      >  SRR4302545.2886133/1‑94 (MQ=60)
                                           GACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAG                                                     <  SRR4302545.2055544/94‑1 (MQ=60)
                                           GACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAG                                                     <  SRR4302545.4651572/94‑1 (MQ=60)
                                           GACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAG                                                     <  SRR4302545.798728/94‑1 (MQ=60)
                                           GACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAG                                                     <  SRR4302545.8104844/94‑1 (MQ=60)
                                            ACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGA                                                    >  SRR4302545.6100278/1‑94 (MQ=60)
                                            ACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGA                                                    <  SRR4302545.10001659/94‑1 (MQ=60)
                                            ACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGA                                                    >  SRR4302545.10706315/1‑94 (MQ=60)
                                             TGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   >  SRR4302545.5348980/1‑94 (MQ=60)
                                             CGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   <  SRR4302545.5844018/94‑1 (MQ=60)
                                             CGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   <  SRR4302545.2093684/94‑1 (MQ=60)
                                             CGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   >  SRR4302545.1612056/1‑94 (MQ=60)
                                             CGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   <  SRR4302545.6950052/94‑1 (MQ=60)
                                             AGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGAC                                                   <  SRR4302545.9366373/94‑1 (MQ=60)
                                               AACGGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGA                                                 <  SRR4302545.2809774/94‑1 (MQ=60)
                                                ACTGGTATAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAT                                                <  SRR4302545.5499862/94‑1 (MQ=60)
                                                ACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAT                                                >  SRR4302545.3646111/1‑94 (MQ=60)
                                                ACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAT                                                <  SRR4302545.1781078/94‑1 (MQ=60)
                                                 CTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATT                                               >  SRR4302545.6084677/1‑94 (MQ=60)
                                                 CTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATT                                               <  SRR4302545.6625361/94‑1 (MQ=60)
                                                  TGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTA                                              <  SRR4302545.6277753/94‑1 (MQ=60)
                                                  TGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTA                                              <  SRR4302545.5102361/94‑1 (MQ=60)
                                                    GTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAT                                            <  SRR4302545.2285230/94‑1 (MQ=60)
                                                    GTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAT                                            >  SRR4302545.9370168/1‑94 (MQ=60)
                                                    GTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAAT                                            >  SRR4302545.1446547/1‑94 (MQ=60)
                                                     TAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATT                                           >  SRR4302545.3921180/1‑94 (MQ=60)
                                                      AGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTT                                          >  SRR4302545.9468349/1‑94 (MQ=60)
                                                      AGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTT                                          <  SRR4302545.3674592/94‑1 (MQ=60)
                                                      AGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTT                                          >  SRR4302545.5319732/1‑94 (MQ=60)
                                                       GAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTC                                         <  SRR4302545.7065100/94‑1 (MQ=60)
                                                       GAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTC                                         <  SRR4302545.2649091/94‑1 (MQ=60)
                                                       GAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTC                                         <  SRR4302545.9724311/94‑1 (MQ=60)
                                                       GAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTC                                         >  SRR4302545.2075692/1‑94 (MQ=60)
                                                       GAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTC                                         >  SRR4302545.32809/1‑94 (MQ=60)
                                                        AAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCG                                        >  SRR4302545.5145602/1‑94 (MQ=60)
                                                        AAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCG                                        <  SRR4302545.1559188/94‑1 (MQ=60)
                                                         AGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGT                                       >  SRR4302545.6182686/1‑94 (MQ=60)
                                                         AGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGT                                       <  SRR4302545.1820147/94‑1 (MQ=60)
                                                          GTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTT                                      >  SRR4302545.9339541/1‑94 (MQ=60)
                                                          GTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACCATTAATTTCGTT                                      <  SRR4302545.10755609/94‑1 (MQ=60)
                                                            GAGTCCGTCCTCGATCAGTATTCATAAACGT‑CACCTGACGGAAAACGAGCGGCGCCGCGCCAACCGCGCACCGAAAGACGACTAATTTCGTTTT                                    <  SRR4302545.7422295/94‑1 (MQ=53)
                                                            GACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTT                                    >  SRR4302545.10295233/1‑94 (MQ=60)
                                                            GACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTT                                    >  SRR4302545.2892259/1‑94 (MQ=60)
                                                             ACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTT                                   >  SRR4302545.2842352/1‑94 (MQ=60)
                                                             ACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTT                                   >  SRR4302545.450016/1‑94 (MQ=60)
                                                             ACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTT                                   <  SRR4302545.2445113/94‑1 (MQ=60)
                                                             ACTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGACGCCGCGCCAACCGCGCACCGAAAGACGA‑TAATTTCGTTTTTT                                  >  SRR4302545.1118355/1‑94 (MQ=60)
                                                              CTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTT                                  >  SRR4302545.5550811/1‑94 (MQ=60)
                                                              CTCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTT                                  >  SRR4302545.3895354/1‑94 (MQ=60)
                                                               TCCGTCCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTA                                 <  SRR4302545.420295/94‑1 (MQ=60)
                                                               TCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTA                                 <  SRR4302545.3717488/94‑1 (MQ=60)
                                                               TCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTA                                 <  SRR4302545.1288448/94‑1 (MQ=60)
                                                               TCCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTA                                 <  SRR4302545.3577937/94‑1 (MQ=60)
                                                                CCGCCCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAG                                <  SRR4302545.4121619/94‑1 (MQ=60)
                                                                CCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAG                                <  SRR4302545.6234788/94‑1 (MQ=60)
                                                                CCGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAG                                <  SRR4302545.4917058/94‑1 (MQ=60)
                                                                 CGACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGT                               <  SRR4302545.3753221/94‑1 (MQ=60)
                                                                  GACCTCTATCCGTATTCGTATATGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTATTC                              >  SRR4302545.9934987/1‑94 (MQ=60)
                                                                  GACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTC                              >  SRR4302545.2954782/1‑94 (MQ=60)
                                                                  GACCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTC                              >  SRR4302545.6883067/1‑94 (MQ=60)
                                                                   CCCTCTATCCGTATTCGTAAACGTCCC‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCA                             <  SRR4302545.2867676/94‑1 (MQ=60)
                                                                   CCCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCA                             <  SRR4302545.1182227/94‑1 (MQ=60)
                                                                    CCTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAT                            >  SRR4302545.6512078/1‑94 (MQ=60)
                                                                     CTCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATA                           >  SRR4302545.3048872/1‑94 (MQ=60)
                                                                      ttaATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          <  SRR4302545.523200/91‑1 (MQ=60)
                                                                      TCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          <  SRR4302545.3167484/94‑1 (MQ=60)
                                                                      TCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          >  SRR4302545.9350490/1‑94 (MQ=60)
                                                                      TCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          >  SRR4302545.4343200/1‑94 (MQ=60)
                                                                      TCTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAA                          >  SRR4302545.8436111/1‑94 (MQ=60)
                                                                       CTATCCGTATTCGTAAACGT‑CACCTGATGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAA                         >  SRR4302545.2329968/1‑94 (MQ=60)
                                                                       CTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAA                         >  SRR4302545.1516637/1‑94 (MQ=60)
                                                                       CTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAA                         >  SRR4302545.9423744/1‑94 (MQ=60)
                                                                       CTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAA                         <  SRR4302545.5616790/94‑1 (MQ=60)
                                                                       CTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAA                         <  SRR4302545.10819031/94‑1 (MQ=60)
                                                                       CTATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAA                         <  SRR4302545.3701097/94‑1 (MQ=60)
                                                                         ATCCTTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAA                       <  SRR4302545.2457075/94‑1 (MQ=60)
                                                                         ATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCCTAACAA                       >  SRR4302545.4312111/1‑94 (MQ=60)
                                                                         ATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAA                       >  SRR4302545.7664036/1‑94 (MQ=60)
                                                                         ATCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAA                       >  SRR4302545.1661808/1‑94 (MQ=60)
                                                                          TCCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAA                      <  SRR4302545.3234095/94‑1 (MQ=60)
                                                                           CCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAAC                     >  SRR4302545.1117507/1‑94 (MQ=60)
                                                                           CCGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAAC                     <  SRR4302545.10303617/94‑1 (MQ=60)
                                                                            CGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACC                    >  SRR4302545.1249181/1‑94 (MQ=60)
                                                                            CGTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACC                    <  SRR4302545.4692995/94‑1 (MQ=60)
                                                                             GTATTCGTAACCGT‑CACCTGACGGAAAACGATCGTCGCCACGCCAACCGCCCACCGAAAGGCGATTAATTTCGTTTTTTAGTCATAAAAAACCT                   <  SRR4302545.10622349/94‑1 (MQ=60)
                                                                             GTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAANCCT                   >  SRR4302545.7897589/1‑94 (MQ=60)
                                                                             GTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCT                   >  SRR4302545.10099180/1‑94 (MQ=60)
                                                                             GTATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCT                   >  SRR4302545.6690428/1‑94 (MQ=60)
                                                                              TATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCTCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTG                  <  SRR4302545.577627/94‑1 (MQ=60)
                                                                              TATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTG                  >  SRR4302545.3540164/1‑94 (MQ=60)
                                                                              TATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTG                  <  SRR4302545.9466066/94‑1 (MQ=60)
                                                                              TATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTG                  <  SRR4302545.9262160/94‑1 (MQ=60)
                                                                              TATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTG                  <  SRR4302545.7600845/94‑1 (MQ=60)
                                                                              TATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTG                  <  SRR4302545.10416576/94‑1 (MQ=60)
                                                                               ATTCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGC                 >  SRR4302545.9051830/1‑94 (MQ=60)
                                                                                 TCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCA               <  SRR4302545.6468534/94‑1 (MQ=60)
                                                                                 TCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCA               >  SRR4302545.9091155/1‑94 (MQ=60)
                                                                                 TCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCA               <  SRR4302545.5673958/94‑1 (MQ=60)
                                                                                 TCGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCA               <  SRR4302545.3178189/94‑1 (MQ=60)
                                                                                  CGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAG              <  SRR4302545.8913733/94‑1 (MQ=60)
                                                                                  CGTAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAG              <  SRR4302545.4337844/94‑1 (MQ=60)
                                                                                    TAAACGTCCC‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCG            <  SRR4302545.3401140/94‑1 (MQ=60)
                                                                                    TAAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCG            >  SRR4302545.1891832/1‑94 (MQ=60)
                                                                                     AAACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGA           <  SRR4302545.8240829/94‑1 (MQ=60)
                                                                                      AACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          >  SRR4302545.10597216/1‑94 (MQ=60)
                                                                                      AACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          <  SRR4302545.982216/94‑1 (MQ=60)
                                                                                      AACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          >  SRR4302545.10187384/1‑94 (MQ=60)
                                                                                      AACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          >  SRR4302545.9964774/1‑94 (MQ=60)
                                                                                      AACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          <  SRR4302545.6845643/94‑1 (MQ=60)
                                                                                      AACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAT          <  SRR4302545.7743607/94‑1 (MQ=60)
                                                                                       ACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATG         <  SRR4302545.505776/94‑1 (MQ=60)
                                                                                       ACGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATG         >  SRR4302545.6912666/1‑94 (MQ=60)
                                                                                        CGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGG        <  SRR4302545.2611913/94‑1 (MQ=60)
                                                                                        CGT‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGG        <  SRR4302545.9872661/94‑1 (MQ=60)
                                                                                          T‑CACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCA      >  SRR4302545.5151486/1‑94 (MQ=60)
                                                                                            cacCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAG     <  SRR4302545.3634135/91‑1 (MQ=60)
                                                                                            cacCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAG     <  SRR4302545.1269876/91‑1 (MQ=60)
                                                                                             acCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCCGG    >  SRR4302545.4937156/3‑94 (MQ=60)
                                                                                             acCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG    <  SRR4302545.9100226/92‑1 (MQ=60)
                                                                                             C‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGT   <  SRR4302545.7814062/94‑1 (MQ=60)
                                                                                               CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTT  >  SRR4302545.8273440/1‑94 (MQ=60)
                                                                                               CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGGT  >  SRR4302545.2080199/1‑94 (MQ=60)
                                                                                               |                                                                                             
TATCCGCATGACTCTGGAACAAGCTCTGGAGTTCATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGT‑CA‑CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTT  >  NC_000913/4057666‑4057852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑