Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,478,511 Δ1 bp intergenic (+20/+39) yjgN → / ← valS hypothetical protein/valine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,478,5110T.99.5% 1873.2 / ‑2.2 415intergenic (+20/+39)yjgN/valShypothetical protein/valine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base . (215/198);  minor base C (2/0);  total (217/198)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 444 total aligned reads displayed.

AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTC  >  NC_000913/4478421‑4478600
                                                                                          |                                                                                         
     caacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCg                                                                                 >  2:9631502/1‑94 (MQ=255)
      aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCgg                                                                                <  1:8995709/94‑1 (MQ=255)
      aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCgg                                                                                >  1:7425376/1‑94 (MQ=255)
      aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCgg                                                                                <  2:10373270/94‑1 (MQ=255)
       acCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGc                                                                               <  2:1568503/94‑1 (MQ=255)
       acCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGc                                                                               >  2:821615/1‑94 (MQ=255)
       acCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGG‑GGCATGCTCCGGc                                                                               >  1:7641995/1‑94 (MQ=255)
         cAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCt                                                                             >  1:1644568/1‑94 (MQ=255)
         cAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCt                                                                             >  1:5107257/1‑94 (MQ=255)
            aTAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcg                                                                          >  1:5355112/1‑92 (MQ=255)
            aTAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcg                                                                          <  2:10005129/94‑3 (MQ=255)
              aaaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                                        >  1:6878497/1‑90 (MQ=255)
              aaaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                                        >  1:9125177/1‑90 (MQ=255)
               aaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGttt                                                                      >  1:2728967/1‑94 (MQ=255)
               aaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGttt                                                                      >  2:7739997/1‑94 (MQ=255)
               aaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGttt                                                                      >  1:3569758/1‑94 (MQ=255)
                 ggCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCTT‑CGTTTtc                                                                    >  2:5837079/1‑94 (MQ=255)
                   cttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcat                                                                  <  2:10102579/94‑1 (MQ=255)
                    ttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatc                                                                 >  2:3774178/1‑94 (MQ=255)
                    ttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatc                                                                 >  2:3121836/1‑94 (MQ=255)
                     tcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatca                                                                <  1:10067149/94‑1 (MQ=255)
                      cttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAc                                                               <  2:6946224/94‑1 (MQ=255)
                        tAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTTCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtg                                                             <  1:2776862/94‑1 (MQ=255)
                        tAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtg                                                             <  2:645633/94‑1 (MQ=255)
                         aGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGG‑CGCATGCTCCGGCCTT‑CGTTTTCATCACtgt                                                            >  2:2030755/1‑94 (MQ=255)
                          gCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtgtg                                                           >  2:9498070/1‑94 (MQ=255)
                           ccAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATCCTCCGGCCTT‑CGTTTTCATCACTgtgt                                                          <  2:7117346/94‑1 (MQ=255)
                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AACAAAGGCCGGA‑GCATGCTACGGCATT‑CGTTTTCATCCCTGTGttt                                                        >  2:1137646/1‑94 (MQ=255)
                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGttt                                                        >  1:8603807/1‑94 (MQ=255)
                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGttt                                                        <  1:6438284/94‑1 (MQ=255)
                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGttt                                                        <  2:7221382/94‑1 (MQ=255)
                              gTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtttt                                                       <  1:6781639/94‑1 (MQ=255)
                              gTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtttt                                                       <  1:5413720/94‑1 (MQ=255)
                              gTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtttt                                                       <  2:6721086/94‑1 (MQ=255)
                              atATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCCCTGTGtttt                                                       <  2:3458770/93‑1 (MQ=255)
                               tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTg                                                      <  2:1705807/94‑1 (MQ=255)
                               tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTg                                                      <  2:898790/94‑1 (MQ=255)
                                aTTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTgt                                                     >  2:2394047/1‑93 (MQ=255)
                                 tttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGAt                                                    <  2:2234275/94‑1 (MQ=255)
                                   tCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTa                                                  <  1:803617/94‑1 (MQ=255)
                                    cACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTAc                                                 >  1:6112370/1‑94 (MQ=255)
                                     aCGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACa                                                >  2:4837782/1‑94 (MQ=255)
                                      cGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                               <  2:9816361/94‑1 (MQ=255)
                                      cGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                               <  1:7901431/94‑1 (MQ=255)
                                       ggggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgc                                              <  1:8386977/94‑1 (MQ=255)
                                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCATTGTGTTTTGATTACAgcg                                             <  1:4399292/94‑1 (MQ=255)
                                        gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCACGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcg                                             <  2:8622911/94‑1 (MQ=255)
                                         ggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcgc                                            <  2:634702/94‑1 (MQ=255)
                                          gAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGcgcg                                           <  1:4156233/94‑1 (MQ=255)
                                           aGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcgg                                          >  2:4711409/1‑94 (MQ=255)
                                           aGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcgg                                          >  2:3713013/1‑94 (MQ=255)
                                            gTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcggc                                         <  1:1769728/94‑1 (MQ=255)
                                               aTGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGAt                                      >  2:5533510/1‑94 (MQ=255)
                                                  ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTCCGGGCTT‑CGTTTTCATTACTGTGTTTTTATTACAGCGCGGGGATaaa                                   >  1:7816260/1‑93 (MQ=255)
                                                  ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                                   >  1:10012170/1‑94 (MQ=255)
                                                  ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                                   >  1:2496890/1‑94 (MQ=255)
                                                  ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                                   >  2:2859163/1‑94 (MQ=255)
                                                  ccTTCTTGACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                                   <  1:9831197/94‑1 (MQ=255)
                                                   cttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACa                                  >  2:7895356/1‑94 (MQ=255)
                                                    ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAg                                 >  1:8799899/1‑94 (MQ=255)
                                                     tcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGTCCT‑CGTTTTCATCACTGTGTTTTGATTATAGCGCGGCGATAACAGc                                >  2:9372960/1‑94 (MQ=255)
                                                     tcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGc                                >  1:462294/1‑94 (MQ=255)
                                                      cttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGcc                               >  1:2284893/1‑94 (MQ=255)
                                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCct                              <  2:1846295/94‑1 (MQ=255)
                                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCct                              <  1:8723449/94‑1 (MQ=255)
                                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCct                              <  2:6038015/94‑1 (MQ=255)
                                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGCCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCct                              >  1:8876075/1‑94 (MQ=255)
                                                        ttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctg                             >  2:519996/1‑94 (MQ=255)
                                                        ttACCATTTCTGTAAGAG‑TTAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGActg                             >  2:2704563/1‑94 (MQ=255)
                                                         tACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgc                            <  1:2013584/94‑1 (MQ=255)
                                                          aCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCCGCGATAACAGCctgct                           >  2:3924471/1‑94 (MQ=255)
                                                          aCCATCTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCC‑TGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgct                           <  2:4051504/94‑1 (MQ=255)
                                                           ccATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgctg                          <  1:125902/94‑1 (MQ=255)
                                                           ccATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgctg                          >  2:4404447/1‑94 (MQ=255)
                                                           ccATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgctg                          <  1:7006491/94‑1 (MQ=255)
                                                            cATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGt                         >  2:1316852/1‑94 (MQ=255)
                                                            cATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGt                         >  1:8265611/1‑94 (MQ=255)
                                                            cATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGt                         >  1:344980/1‑94 (MQ=255)
                                                               ttCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCa                      <  1:3176354/94‑1 (MQ=255)
                                                                 cTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAt                    >  2:5415149/1‑94 (MQ=255)
                                                                  tGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCCCCTGTTCAATc                   >  1:10288037/1‑94 (MQ=255)
                                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCa                  >  2:6851749/1‑94 (MQ=255)
                                                                    tAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCAGCGATAACAGCCTGCTGTTCAATCAg                 >  2:5009712/1‑94 (MQ=255)
                                                                     aaGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGt                >  2:5626119/1‑94 (MQ=255)
                                                                     aaGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGt                <  2:9277203/94‑1 (MQ=255)
                                                                         agataaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttc             <  2:9186574/90‑1 (MQ=255)
                                                                         agataaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttc             >  2:8328872/5‑94 (MQ=255)
                                                                          gataaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcg            <  1:10385532/91‑1 (MQ=255)
                                                                           ataaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCTCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgc           <  1:7233159/92‑1 (MQ=255)
                                                                           ataaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgc           >  1:4918932/3‑94 (MQ=255)
                                                                             aaaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCGCTGTGTTTTGATTCCAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctt         <  1:7203936/94‑1 (MQ=255)
                                                                              aaaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgcttt        <  1:9335505/94‑1 (MQ=255)
                                                                               aaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctttc       >  1:1543487/1‑94 (MQ=255)
                                                                               aaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctttc       >  1:5626546/1‑94 (MQ=255)
                                                                               aaaaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctttc       >  1:633655/1‑94 (MQ=255)
                                                                                 aaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGGTGTTCAATCAGtttcgctttcgc     >  1:5825798/1‑94 (MQ=255)
                                                                                 aaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctttcgc     >  2:8842536/1‑94 (MQ=255)
                                                                                 aaGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtttcgctttcgc     >  1:3837882/1‑94 (MQ=255)
                                                                                   ggCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTtcgctttcgctt   <  1:1173192/94‑1 (MQ=255)
                                                                                    gCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTc  >  1:9651674/1‑94 (MQ=255)
                                                                                          |                                                                                         
AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTC  >  NC_000913/4478421‑4478600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 268 total aligned reads displayed.
GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  >  NC_000913/4478432‑4478620
                                                                                               |                                                                                             
GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGACGGA‑GCATGCTCCGGCCTTC                                                                                               >  SRR4302545.5248533/1‑94 (MQ=60)
  TAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGT                                                                                            >  SRR4302545.2818855/1‑94 (MQ=60)
   AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                                           >  SRR4302545.7067536/1‑94 (MQ=60)
   AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                                           >  SRR4302545.9393001/1‑94 (MQ=60)
   AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                                           <  SRR4302545.4770997/94‑1 (MQ=60)
   AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                                           >  SRR4302545.8800344/1‑94 (MQ=60)
   AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                                           <  SRR4302545.9239539/94‑1 (MQ=60)
    AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AACAAAGGCCGGA‑GCATCCTCCGGCCTT‑CGTTT                                                                                          >  SRR4302545.8370453/1‑94 (MQ=60)
    AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                                          <  SRR4302545.3836036/94‑1 (MQ=60)
    AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                                          >  SRR4302545.2794788/1‑94 (MQ=60)
    AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                                          >  SRR4302545.3656956/1‑94 (MQ=60)
    AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                                          >  SRR4302545.8370452/1‑94 (MQ=60)
     AGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTT                                                                                         <  SRR4302545.707980/94‑1 (MQ=60)
     AGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTT                                                                                         >  SRR4302545.3014949/1‑94 (MQ=60)
      GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                                        <  SRR4302545.7981346/94‑1 (MQ=60)
      GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                                        <  SRR4302545.3562707/94‑1 (MQ=60)
      GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                                        <  SRR4302545.7441021/94‑1 (MQ=60)
      GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                                        <  SRR4302545.2456981/94‑1 (MQ=60)
       GCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGACGGA‑GCATGCTCCGGCCTT‑CGTTTTCA                                                                                       >  SRR4302545.4319547/1‑94 (MQ=60)
        CTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAT                                                                                      >  SRR4302545.3595892/1‑94 (MQ=60)
          TCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCA                                                                                    <  SRR4302545.10365048/94‑1 (MQ=60)
          TCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCA                                                                                    <  SRR4302545.4643785/94‑1 (MQ=60)
           CTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAC                                                                                   >  SRR4302545.9343466/1‑94 (MQ=60)
             TAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTTCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTG                                                                                 <  SRR4302545.2843874/94‑1 (MQ=60)
             TAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTG                                                                                 >  SRR4302545.1658125/1‑94 (MQ=60)
                CCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGT                                                                              >  SRR4302545.6712344/1‑94 (MQ=60)
                CCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGT                                                                              <  SRR4302545.1415562/94‑1 (MQ=60)
                 CAGTATTTCACGGGGAGTTATGTCTGCTTAACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTT                                                                             <  SRR4302545.2394860/94‑1 (MQ=60)
                 CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTT                                                                             >  SRR4302545.6139899/1‑94 (MQ=60)
                 CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTT                                                                             >  SRR4302545.4851312/1‑94 (MQ=60)
                  AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                                            >  SRR4302545.8852786/1‑94 (MQ=60)
                  AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                                            >  SRR4302545.7044915/1‑94 (MQ=60)
                  AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                                            <  SRR4302545.6614307/94‑1 (MQ=60)
                   GTATTTCACGGTGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                                           <  SRR4302545.5555072/94‑1 (MQ=60)
                   GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                                           <  SRR4302545.5555071/94‑1 (MQ=60)
                   GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                                           <  SRR4302545.7167538/94‑1 (MQ=60)
                   GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                                           <  SRR4302545.9009980/94‑1 (MQ=60)
                   GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                                           >  SRR4302545.3934174/1‑94 (MQ=60)
                   GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                                           <  SRR4302545.9107684/94‑1 (MQ=60)
                    TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                                          <  SRR4302545.5520357/94‑1 (MQ=60)
                    TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                                          <  SRR4302545.7431076/94‑1 (MQ=60)
                    TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                                          <  SRR4302545.10167724/94‑1 (MQ=60)
                    TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                                          >  SRR4302545.10060917/1‑94 (MQ=60)
                    TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                                          <  SRR4302545.5520358/94‑1 (MQ=60)
                        TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTA                                                                      <  SRR4302545.821659/94‑1 (MQ=60)
                            GGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGC                                                                  <  SRR4302545.8629770/94‑1 (MQ=60)
                             GGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCATTGTGTTTTGATTACAGCG                                                                 <  SRR4302545.4510843/94‑1 (MQ=60)
                                AGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGG                                                              <  SRR4302545.7590139/94‑1 (MQ=60)
                                AGTTATGCCTTCTATACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTATGATTACAGCGCGG                                                              <  SRR4302545.7590138/94‑1 (MQ=60)
                                 GTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGC                                                             <  SRR4302545.1812145/94‑1 (MQ=60)
                                   TATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGA                                                           >  SRR4302545.7398231/1‑94 (MQ=60)
                                   TATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGA                                                           >  SRR4302545.2584163/1‑94 (MQ=60)
                                    ATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGAT                                                          <  SRR4302545.7395184/94‑1 (MQ=60)
                                      GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                                        <  SRR4302545.1270003/94‑1 (MQ=60)
                                       CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTCCGGGCTT‑CGTTTTCATTACTGTGTTTTTATTACAGCGCGGGGATAAA                                                       >  SRR4302545.8037604/1‑94 (MQ=47)
                                       CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                                       >  SRR4302545.2557249/1‑94 (MQ=60)
                                       CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                                       >  SRR4302545.10308951/1‑94 (MQ=60)
                                       CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGGTTTGATTACAGCGCGGCGATAAC                                                       >  SRR4302545.7416427/1‑94 (MQ=60)
                                       CCTTCTTGACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                                       <  SRR4302545.10123296/94‑1 (MQ=60)
                                        CTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACA                                                      >  SRR4302545.6810329/1‑94 (MQ=60)
                                         TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAG                                                     <  SRR4302545.5696386/94‑1 (MQ=60)
                                         TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAG                                                     <  SRR4302545.10552663/94‑1 (MQ=60)
                                          TCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGC                                                    >  SRR4302545.472606/1‑94 (MQ=60)
                                          TCTGTACCATGTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGC                                                    <  SRR4302545.6650533/94‑1 (MQ=60)
                                           CTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCC                                                   <  SRR4302545.8227395/94‑1 (MQ=60)
                                            TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  <  SRR4302545.9347313/94‑1 (MQ=60)
                                            TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  >  SRR4302545.6299308/1‑94 (MQ=60)
                                            TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  >  SRR4302545.9133973/1‑94 (MQ=60)
                                            TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  <  SRR4302545.8976613/94‑1 (MQ=60)
                                            TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGCCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  >  SRR4302545.9133974/1‑94 (MQ=60)
                                            TTTACAATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  <  SRR4302545.4289211/94‑1 (MQ=60)
                                            TTGACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                                  <  SRR4302545.10322451/94‑1 (MQ=60)
                                             TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTG                                                 <  SRR4302545.9696707/94‑1 (MQ=60)
                                             TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTG                                                 <  SRR4302545.8591082/94‑1 (MQ=60)
                                             TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTG                                                 <  SRR4302545.3245349/94‑1 (MQ=60)
                                              TACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGC                                                >  SRR4302545.1092284/1‑94 (MQ=60)
                                              TACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGC                                                <  SRR4302545.2063322/94‑1 (MQ=60)
                                               ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCT                                               >  SRR4302545.3095858/1‑94 (MQ=60)
                                               ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCT                                               <  SRR4302545.5113549/94‑1 (MQ=60)
                                                CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                                              <  SRR4302545.128743/94‑1 (MQ=60)
                                                CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                                              >  SRR4302545.9040535/1‑94 (MQ=60)
                                                CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                                              <  SRR4302545.7199808/94‑1 (MQ=60)
                                                CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                                              <  SRR4302545.2312412/94‑1 (MQ=60)
                                                 CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                                             >  SRR4302545.352385/1‑94 (MQ=60)
                                                 CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                                             >  SRR4302545.8504842/1‑94 (MQ=60)
                                                 CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                                             >  SRR4302545.10894326/1‑94 (MQ=60)
                                                  ATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTT                                            <  SRR4302545.4029663/94‑1 (MQ=60)
                                                  ATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTT                                            >  SRR4302545.1265281/1‑94 (MQ=60)
                                                   TTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTC                                           >  SRR4302545.6542702/1‑94 (MQ=60)
                                                   TTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTC                                           >  SRR4302545.1629322/1‑94 (MQ=60)
                                                    TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                                          <  SRR4302545.9833526/94‑1 (MQ=60)
                                                    TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                                          <  SRR4302545.10200092/94‑1 (MQ=60)
                                                    TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                                          >  SRR4302545.1682285/1‑94 (MQ=60)
                                                    TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                                          <  SRR4302545.1954927/94‑1 (MQ=60)
                                                    TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                                          <  SRR4302545.3254309/94‑1 (MQ=60)
                                                    TTCTGTAAGAGA‑AAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                                          <  SRR4302545.2093529/94‑1 (MQ=60)
                                                     TCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAA                                         <  SRR4302545.8291668/94‑1 (MQ=60)
                                                      CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAT                                        >  SRR4302545.1714998/1‑94 (MQ=60)
                                                      CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGAGATAACAGCCTGCTGTTCAAT                                        >  SRR4302545.10012994/1‑94 (MQ=60)
                                                       TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                                       <  SRR4302545.10542209/94‑1 (MQ=60)
                                                        GTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCA                                      <  SRR4302545.9223112/94‑1 (MQ=60)
                                                         TAAGAGAT‑AAAAAATGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAG                                     <  SRR4302545.620783/94‑1 (MQ=60)
                                                         TAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAG                                     >  SRR4302545.10313049/1‑94 (MQ=60)
                                                          AAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGT                                    >  SRR4302545.8623278/1‑94 (MQ=60)
                                                           AGAGATAAAAAAGGGCGGG‑‑GCATGCTCCCGGCTTCCG‑TTTCATCACTGTGTTTTGATTTCAGCGCGGCGATAAAAGCCTGCTGTTTAATCAGAT                                   >  SRR4302545.2295608/1‑94 (MQ=33)
                                                           AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT                                   >  SRR4302545.5067622/1‑94 (MQ=60)
                                                            GAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT                                  >  SRR4302545.5313225/1‑94 (MQ=60)
                                                            GAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT                                  <  SRR4302545.2745127/94‑1 (MQ=60)
                                                             aagatAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT                                  <  SRR4302545.2584214/89‑1 (MQ=60)
                                                             AGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTC                                 <  SRR4302545.1242507/94‑1 (MQ=60)
                                                              GAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCG                                <  SRR4302545.10691702/94‑1 (MQ=60)
                                                                atAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGC                               >  SRR4302545.7132018/3‑94 (MQ=60)
                                                                atAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGC                               >  SRR4302545.5043728/3‑94 (MQ=60)
                                                                  AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATTACTGTGTTTTTATTACAGCGCGGCGATAACAGCCCGCTGTTCTATCCGTTTCTCTT                             >  SRR4302545.6257659/1‑94 (MQ=56)
                                                                  AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCGCTGTGTTTTGATTCCAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTT                             <  SRR4302545.7404721/94‑1 (MQ=60)
                                                                  AAAAAAGGCCGG‑GGCATGCTCCCGCCTT‑CGTTTTCATCACTGTGTTTTTATTTCAGCGCCGCCATAACCACCTGCTGTTCAATCAGTTTTGCTT                             >  SRR4302545.6257658/1‑94 (MQ=24)
                                                                   AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT                            >  SRR4302545.1354540/1‑94 (MQ=60)
                                                                   AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT                            <  SRR4302545.9609212/94‑1 (MQ=60)
                                                                   AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT                            >  SRR4302545.1690662/1‑94 (MQ=60)
                                                                   AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT                            <  SRR4302545.9609211/94‑1 (MQ=60)
                                                                   AAAAAGGCCGG‑GGCATGCTCCGGCCTT‑CGATTTCATCACTGTGTTTTGATTACAGCGCGGAGATAACAGACTGCTGTTCAATCAGTTTCGCTTT                            >  SRR4302545.6319218/1‑94 (MQ=60)
                                                                    AAAAGGCGGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC                           <  SRR4302545.4322562/94‑1 (MQ=60)
                                                                    AAAAGGCCGGA‑GGATGCTCCGGCCTT‑CGTTTTCATCACTGTGGTTTTATTACAGCGCGGCGATAACAGCCCGCTGTTCCATCCATTTCGCTTTC                           >  SRR4302545.80882/1‑94 (MQ=43)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC                           >  SRR4302545.5776126/1‑94 (MQ=60)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC                           >  SRR4302545.5776127/1‑94 (MQ=60)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC                           <  SRR4302545.4841110/94‑1 (MQ=60)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC                           >  SRR4302545.1579709/1‑94 (MQ=60)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC                           >  SRR4302545.9017400/1‑94 (MQ=60)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC                           >  SRR4302545.647852/1‑94 (MQ=60)
                                                                    AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGGTTTTATCACTGTGTTTTGATTACAGCGCGGCGATTACAGCCTGGTGTTCAATCAGTTTCGCTTTG                           >  SRR4302545.80597/1‑94 (MQ=60)
                                                                      AAGGGCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC                         >  SRR4302545.10675140/1‑94 (MQ=60)
                                                                      AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGGTGTTCAATCAGTTTCGCTTTCGC                         >  SRR4302545.5982594/1‑94 (MQ=60)
                                                                      AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC                         >  SRR4302545.3933589/1‑94 (MQ=60)
                                                                      AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC                         >  SRR4302545.8926659/1‑94 (MQ=60)
                                                                      AAGGACGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC                         >  SRR4302545.10675139/1‑94 (MQ=60)
                                                                       AGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCT                        >  SRR4302545.794047/1‑94 (MQ=60)
                                                                       AGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCT                        >  SRR4302545.8976088/1‑94 (MQ=60)
                                                                        GGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTT                       >  SRR4302545.1283121/1‑94 (MQ=60)
                                                                         GCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTC                      <  SRR4302545.8869553/94‑1 (MQ=60)
                                                                          gcccgagcattCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCATGCTGTTCAATCAGTTTCGCTTTCGCGTC                      <  SRR4302545.8869552/83‑1 (MQ=60)
                                                                          CCGGA‑GCTTGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCC                     <  SRR4302545.4640493/94‑1 (MQ=60)
                                                                          CCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCC                     <  SRR4302545.6999011/94‑1 (MQ=60)
                                                                          CCGGA‑GCATGCTCCGGCCTT‑CGTCTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGTCTGCTGTTCAATCAGTTTCGCTTTCGCTTCC                     <  SRR4302545.5996348/94‑1 (MQ=60)
                                                                           CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG                    >  SRR4302545.6147124/1‑94 (MQ=60)
                                                                           CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG                    <  SRR4302545.4977636/94‑1 (MQ=60)
                                                                           CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG                    >  SRR4302545.1974184/1‑94 (MQ=60)
                                                                           CGGA‑GCATGCTCCGGCCTT‑CGTTATCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG                    >  SRR4302545.6908362/1‑94 (MQ=60)
                                                                           CGGA‑GCATGCTCCGGCCTT‑CGCTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCCCTTCCG                    >  SRR4302545.8652014/1‑94 (MQ=60)
                                                                            GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC                   >  SRR4302545.8560454/1‑94 (MQ=60)
                                                                            GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC                   >  SRR4302545.8237399/1‑94 (MQ=60)
                                                                            GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC                   >  SRR4302545.2804184/1‑94 (MQ=60)
                                                                            GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC                   >  SRR4302545.2084810/1‑94 (MQ=60)
                                                                            GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC                   <  SRR4302545.9322217/94‑1 (MQ=60)
                                                                            GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC                   >  SRR4302545.3546678/1‑94 (MQ=60)
                                                                            GGA‑GCATGCTCCGGCCTT‑CGATTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCTATCAGTTTCGCTTTCGCTTCCGA                   >  SRR4302545.7448539/1‑94 (MQ=60)
                                                                             gggccaccccCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC                   <  SRR4302545.7754304/84‑1 (MQ=60)
                                                                             ggagaATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCCGTTTCGCTTTCGCTTCCGC                   >  SRR4302545.7448538/6‑94 (MQ=60)
                                                                              gaGCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCA                  >  SRR4302545.86455/3‑94 (MQ=60)
                                                                               AGCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCAT                 <  SRR4302545.4604273/94‑1 (MQ=60)
                                                                                GCATTCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATA                <  SRR4302545.5935203/94‑1 (MQ=60)
                                                                                GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATA                <  SRR4302545.1393595/94‑1 (MQ=60)
                                                                                GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATA                >  SRR4302545.6402323/1‑94 (MQ=60)
                                                                                 CATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAG               <  SRR4302545.5884583/94‑1 (MQ=60)
                                                                                 CATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGAGTACAGCGCGGCGATACCAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAG               <  SRR4302545.5884584/94‑1 (MQ=60)
                                                                                  ATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGC              >  SRR4302545.10398124/1‑94 (MQ=60)
                                                                                  ATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGC              >  SRR4302545.6072998/1‑94 (MQ=60)
                                                                                  ATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGC              >  SRR4302545.10398123/1‑94 (MQ=60)
                                                                                  ATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGC              >  SRR4302545.4187202/1‑94 (MQ=60)
                                                                                  ATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGC              >  SRR4302545.250551/1‑94 (MQ=60)
                                                                                  ATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGC              >  SRR4302545.1533509/1‑94 (MQ=60)
                                                                                  ATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGC              >  SRR4302545.4370557/1‑94 (MQ=60)
                                                                                   TGCTCCGGCCTT‑CGTTTTCATAACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCC             >  SRR4302545.10492650/1‑94 (MQ=60)
                                                                                    GCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCT            <  SRR4302545.5559616/94‑1 (MQ=60)
                                                                                     CTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTT           >  SRR4302545.3470630/1‑94 (MQ=60)
                                                                                     CTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTT           >  SRR4302545.2726273/1‑94 (MQ=60)
                                                                                     CTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTT           >  SRR4302545.4932085/1‑94 (MQ=60)
                                                                                     CTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTT           <  SRR4302545.4492048/94‑1 (MQ=60)
                                                                                     CTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTT           <  SRR4302545.1543319/94‑1 (MQ=60)
                                                                                      TCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTC          >  SRR4302545.2283307/1‑94 (MQ=60)
                                                                                      TCCGGCCTT‑CGTTTTCATCACTGTGATTTGATTAGAGCGCGGCGATAACAGCCTGCTGCTCAATCAGTTTTGCTTTCGCTTCCGCATAGtctgc          >  SRR4302545.6895029/1‑89 (MQ=60)
                                                                                       CCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCC         >  SRR4302545.5442309/1‑94 (MQ=60)
                                                                                       CCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCC         >  SRR4302545.7038679/1‑94 (MQ=60)
                                                                                        CGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCA        >  SRR4302545.850771/1‑94 (MQ=60)
                                                                                         GGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAG       <  SRR4302545.3226606/94‑1 (MQ=60)
                                                                                         GGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAG       >  SRR4302545.10926228/1‑94 (MQ=60)
                                                                                         GGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAG       >  SRR4302545.3492793/1‑94 (MQ=60)
                                                                                          GCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGC      >  SRR4302545.9220503/1‑94 (MQ=60)
                                                                                           CCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCT     >  SRR4302545.4205876/1‑94 (MQ=60)
                                                                                           CCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCT     <  SRR4302545.6348370/94‑1 (MQ=60)
                                                                                           CCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCT     >  SRR4302545.1109265/1‑94 (MQ=60)
                                                                                           CCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCT     >  SRR4302545.5912479/1‑94 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    <  SRR4302545.1110656/94‑1 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    >  SRR4302545.3670929/1‑94 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    <  SRR4302545.1110658/94‑1 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    >  SRR4302545.1202653/1‑94 (MQ=60)
                                                                                            CTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTT    >  SRR4302545.3045770/1‑94 (MQ=60)
                                                                                              TTCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTC   <  SRR4302545.7129601/94‑1 (MQ=60)
                                                                                               TCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  <  SRR4302545.7449590/94‑1 (MQ=60)
                                                                                               TCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  <  SRR4302545.3744731/94‑1 (MQ=60)
                                                                                               TCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  >  SRR4302545.10019093/1‑94 (MQ=60)
                                                                                               |                                                                                             
GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCATAGCCTTCCAGCTTCT  >  NC_000913/4478432‑4478620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑