Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,573,190 Δ1 bp coding (1487/1542 nt) yjiU → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,573,1890G.99.8% 2039.0 / ‑4.3 445coding (1486/1542 nt)yjiUhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (236/208);  minor base C (1/0);  total (237/208)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 479 total aligned reads displayed.

ATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCC  >  NC_000913/4573096‑4573278
                                                                                             |                                                                                         
attatCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAg                                                                                           >  1:6326364/1‑94 (MQ=255)
 ttatCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAga                                                                                          <  2:8100641/94‑2 (MQ=255)
   atCTATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAgagc                                                                                        <  2:965752/94‑4 (MQ=255)
   atCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAgagc                                                                                        <  1:945563/94‑4 (MQ=255)
     cAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCat                                                                                     <  1:4695833/94‑1 (MQ=255)
       aTACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCatat                                                                                   >  2:5045894/1‑94 (MQ=255)
        tACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATg                                                                                  <  1:10331369/94‑1 (MQ=255)
         aCATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGa                                                                                 <  2:6104959/94‑1 (MQ=255)
          cATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGAc                                                                                >  1:1940810/1‑94 (MQ=255)
          cATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGAc                                                                                >  1:6538285/1‑94 (MQ=255)
          cATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGAc                                                                                >  2:6316056/1‑94 (MQ=255)
           aTGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGGAA‑GAGCAAATGACt                                                                               >  2:9321967/1‑94 (MQ=255)
           aTGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACt                                                                               >  1:3749470/1‑94 (MQ=255)
            tGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACtt                                                                              <  1:1281145/94‑1 (MQ=255)
             ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACttt                                                                             <  1:2590602/94‑1 (MQ=255)
              gCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACtttt                                                                            >  1:160984/1‑94 (MQ=255)
                 gAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCt                                                                         >  1:8078127/1‑94 (MQ=255)
                   aagaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCttt                                                                       <  2:106335/94‑1 (MQ=255)
                   aagaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCttt                                                                       <  2:4937878/94‑1 (MQ=255)
                   aagaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCttt                                                                       <  1:2364238/94‑1 (MQ=255)
                     aaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTaa                                                                     <  2:3529527/93‑1 (MQ=255)
                      aagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAAt                                                                    <  2:5628022/94‑1 (MQ=255)
                       agCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATc                                                                   <  2:7785233/94‑1 (MQ=255)
                       agCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATc                                                                   >  1:533763/1‑94 (MQ=255)
                        gCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCa                                                                  <  2:7113649/94‑1 (MQ=255)
                          aTGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACg                                                                >  1:9349767/1‑94 (MQ=255)
                          aTGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACg                                                                >  2:6260177/1‑94 (MQ=255)
                          aTGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACg                                                                <  2:6965260/94‑1 (MQ=255)
                          aTGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACg                                                                <  2:6504286/94‑1 (MQ=255)
                           tGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACgg                                                               >  1:415013/1‑94 (MQ=255)
                           tGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACgg                                                               <  1:7752262/94‑1 (MQ=255)
                              gCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATg                                                            >  1:5930065/1‑94 (MQ=255)
                               cccAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGc                                                           <  1:5047948/94‑1 (MQ=255)
                                ccAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGcc                                                          >  2:8486906/1‑94 (MQ=255)
                                 cAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCt                                                         >  2:2839547/1‑94 (MQ=255)
                                 cAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCt                                                         >  1:2875428/1‑94 (MQ=255)
                                  aaCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTg                                                        <  1:4075732/94‑1 (MQ=255)
                                  aaCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTg                                                        <  2:7321305/94‑1 (MQ=255)
                                   aCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTgg                                                       <  2:5226438/94‑1 (MQ=255)
                                   aCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTgg                                                       >  2:3130129/1‑94 (MQ=255)
                                   aCTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTgg                                                       <  1:1198391/94‑1 (MQ=255)
                                       aCCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGAttt                                                   >  2:5490133/1‑94 (MQ=255)
                                       aCCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGAttt                                                   <  2:9858423/94‑1 (MQ=255)
                                        ccGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTg                                                  >  1:2078968/1‑94 (MQ=255)
                                          ggACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGcc                                                >  1:7385664/1‑94 (MQ=255)
                                           gACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGccc                                               >  1:354921/1‑94 (MQ=255)
                                            gccGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCg                                              <  2:7626804/93‑1 (MQ=255)
                                            aCCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCg                                              <  2:2642932/94‑1 (MQ=255)
                                             ccGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCgg                                             >  2:5775347/1‑94 (MQ=255)
                                             ccGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCgg                                             >  2:180203/1‑94 (MQ=255)
                                             ccGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCG‑AACGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCgg                                             >  1:84198/1‑94 (MQ=255)
                                              cGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGt                                            >  1:2264879/1‑94 (MQ=255)
                                               gCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTa                                           >  1:7675015/1‑94 (MQ=255)
                                               ccTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTa                                           <  2:9083120/93‑1 (MQ=255)
                                                cTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTaa                                          <  2:4185532/94‑1 (MQ=255)
                                                cTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTaa                                          >  2:1536290/1‑94 (MQ=255)
                                                cTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTaa                                          <  1:8300422/94‑1 (MQ=255)
                                                  tGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACTGATGCCTGGATTTGCCCGGTAAcc                                        >  2:9397118/1‑94 (MQ=255)
                                                   gCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCa                                       >  1:3146414/1‑94 (MQ=255)
                                                     aGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAAt                                     <  2:1282974/94‑1 (MQ=255)
                                                      gTACTGAAACCCGATGGCAACCGTTTTTATTGACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATa                                    <  2:3415190/94‑1 (MQ=255)
                                                       tACTGAAACCCGATGGCAACCGTTTGTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATaa                                   >  1:10148335/1‑94 (MQ=255)
                                                        aCTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATaaa                                  <  1:9810681/94‑1 (MQ=255)
                                                        aCTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATaaa                                  >  2:5882961/1‑94 (MQ=255)
                                                         cTGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATaaaa                                 <  2:10465939/94‑1 (MQ=255)
                                                          tGAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAAt                                >  1:2976907/1‑94 (MQ=255)
                                                           gAAACCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                               >  2:10522571/1‑94 (MQ=255)
                                                            aaaCCCGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATcc                              >  2:10012473/1‑94 (MQ=255)
                                                               cccGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTgg                           <  1:4320131/94‑1 (MQ=255)
                                                                ccGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGa                          >  1:1042302/1‑94 (MQ=255)
                                                                 cGATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGAt                         >  2:1827023/1‑94 (MQ=255)
                                                                  gATGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATa                        >  2:8350609/1‑94 (MQ=255)
                                                                    tGGCAACTGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACg                      >  2:3737012/1‑94 (MQ=255)
                                                                    tGGCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACg                      <  2:4515366/94‑1 (MQ=255)
                                                                     ggCAACCGTTTTTATTTACCGCAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACgg                     >  2:675512/1‑94 (MQ=255)
                                                                     ggCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACgg                     <  1:8612105/94‑1 (MQ=255)
                                                                     ggCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACgg                     <  2:10192870/94‑1 (MQ=255)
                                                                      gCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGc                    <  1:5775017/94‑1 (MQ=255)
                                                                      gCAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGc                    <  1:6793915/94‑1 (MQ=255)
                                                                       cAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCTGCAACCAATAAAATCCTGGATACggat                   >  2:9228421/1‑92 (MQ=255)
                                                                       cAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCt                   >  1:1430141/1‑94 (MQ=255)
                                                                       cAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCt                   >  1:7082748/1‑94 (MQ=255)
                                                                       cAACCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCt                   <  1:7865353/94‑1 (MQ=255)
                                                                        aaCCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCtt                  >  2:4754605/1‑94 (MQ=255)
                                                                        aaCCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCtt                  >  1:2996466/1‑94 (MQ=255)
                                                                        aaCCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCtt                  <  2:3940984/94‑1 (MQ=255)
                                                                         aCCGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCttt                 <  2:2323327/94‑1 (MQ=255)
                                                                          ccGTTTTTATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCtttt                >  1:4456957/1‑94 (MQ=255)
                                                                             tttttATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTaaa             <  1:5734135/94‑1 (MQ=255)
                                                                             tttttATTTACCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTaaa             >  1:1856649/1‑94 (MQ=255)
                                                                                ttatttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGc          >  1:7209856/1‑94 (MQ=255)
                                                                                ttatttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGc          >  2:8765705/1‑94 (MQ=255)
                                                                                ttatttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGc          <  1:906411/94‑1 (MQ=255)
                                                                                    ttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTaa      >  2:7438936/1‑94 (MQ=255)
                                                                                    ttaCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTaa      >  1:3886720/1‑94 (MQ=255)
                                                                                     taCCGAAA‑GAGTATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAc     <  2:8872489/94‑1 (MQ=255)
                                                                                     taCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAc     <  2:2130589/94‑1 (MQ=255)
                                                                                      aCCGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAcc    >  1:5507966/1‑94 (MQ=255)
                                                                                        cGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAcccc  <  1:8850580/94‑1 (MQ=255)
                                                                                        cGAAA‑GAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAcccc  <  1:8542815/94‑1 (MQ=255)
                                                                                             |                                                                                         
ATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCC  >  NC_000913/4573096‑4573278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 258 total aligned reads displayed.
GTGTTCGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCT  >  NC_000913/4574165‑4574351
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GTGTTCGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGG                                                                                               >  SRR4302545.133269/1‑94 (MQ=60)
GTGTTCGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGG                                                                                               >  SRR4302545.4355114/1‑94 (MQ=60)
 TGTTCGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGT                                                                                              <  SRR4302545.4204372/94‑1 (MQ=60)
 TGTTCGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGT                                                                                              <  SRR4302545.3314166/94‑1 (MQ=60)
  GTTCGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTG                                                                                             <  SRR4302545.8242455/94‑1 (MQ=60)
  GTTCGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTG                                                                                             >  SRR4302545.8753367/1‑94 (MQ=60)
    TCTACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGG                                                                                           <  SRR4302545.9723010/94‑1 (MQ=60)
     CGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGC                                                                                          <  SRR4302545.3169875/94‑1 (MQ=60)
      GACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCT                                                                                         >  SRR4302545.8004449/1‑94 (MQ=60)
      GACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCT                                                                                         >  SRR4302545.3472681/1‑94 (MQ=60)
      GACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCT                                                                                         <  SRR4302545.9188669/94‑1 (MQ=60)
      GACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCT                                                                                         >  SRR4302545.7183371/1‑94 (MQ=60)
      GACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGAGGGCT                                                                                         >  SRR4302545.2619023/1‑94 (MQ=60)
       ACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGGCAGGTATTGAAGATCCGCTTCGATCAGGGGGCGGGCTA                                                                                        >  SRR4302545.8035969/1‑94 (MQ=60)
        CTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTAC                                                                                       >  SRR4302545.5310024/1‑94 (MQ=60)
        CTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTAC                                                                                       >  SRR4302545.6759630/1‑94 (MQ=60)
         TCGCCGCGCGATATCCCTCACCATCGTAAACGGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACT                                                                                      <  SRR4302545.462749/94‑1 (MQ=60)
          CGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTG                                                                                     >  SRR4302545.3347384/1‑94 (MQ=60)
          CGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTG                                                                                     <  SRR4302545.6911064/94‑1 (MQ=60)
          CGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTG                                                                                     >  SRR4302545.1937459/1‑94 (MQ=60)
          CGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTG                                                                                     <  SRR4302545.2884106/94‑1 (MQ=60)
           GCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGG                                                                                    <  SRR4302545.10331871/94‑1 (MQ=60)
           GCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGG                                                                                    >  SRR4302545.7653268/1‑94 (MQ=60)
           GCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAAGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGG                                                                                    >  SRR4302545.659530/1‑94 (MQ=60)
            CCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGC                                                                                   <  SRR4302545.6602479/94‑1 (MQ=60)
            CCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGC                                                                                   <  SRR4302545.6507708/94‑1 (MQ=60)
             CGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCG                                                                                  >  SRR4302545.3403704/1‑94 (MQ=60)
              cctgcataaccctctccagCGTAAACTGGCGGTGACCTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTTCTGGGGC                                                                                 <  SRR4302545.3315058/75‑1 (MQ=60)
               CGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGCGGTGGGCTACTGGCGCA                                                                                <  SRR4302545.6160756/94‑1 (MQ=60)
               CGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGGATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCA                                                                                >  SRR4302545.9590631/1‑94 (MQ=60)
                GCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCAT                                                                               <  SRR4302545.3904716/94‑1 (MQ=60)
                GCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCAT                                                                               <  SRR4302545.4518839/94‑1 (MQ=60)
                 TGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATC                                                                              >  SRR4302545.5641173/1‑94 (MQ=60)
                 CGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGGGGGCTACTGGCGCATC                                                                              >  SRR4302545.965918/1‑94 (MQ=60)
                 CGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATC                                                                              >  SRR4302545.4468014/1‑94 (MQ=60)
                 CGATATACCTCAACATCGTAAACTGACGGTGACTTTTGAGGATAGTCAGGTATTGAAGATCCGCTTCGATCAGGGAGTGGGCTACTGGCGCATC                                                                              <  SRR4302545.9678983/94‑1 (MQ=60)
                  GATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCA                                                                             <  SRR4302545.940678/94‑1 (MQ=60)
                  GATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCA                                                                             >  SRR4302545.10667680/1‑94 (MQ=60)
                    TATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAAC                                                                           >  SRR4302545.593533/1‑94 (MQ=60)
                     ATCCCTCACCATCGTAAACTGACGGTGGCTTTTGAGGATGGTCAGGTATTGAAGATCTGCTTCGATCAGGGGGTGGGCTACTGCCGCATCAACT                                                                          >  SRR4302545.7905685/1‑94 (MQ=60)
                     ATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACT                                                                          >  SRR4302545.1461284/1‑94 (MQ=60)
                       CCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTT                                                                        >  SRR4302545.3318556/1‑94 (MQ=60)
                        CCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTT                                                                       <  SRR4302545.4890945/94‑1 (MQ=60)
                        CCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTT                                                                       >  SRR4302545.10009574/1‑94 (MQ=60)
                        CCTCACCATCGTAAACTGACGGTCACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTT                                                                       <  SRR4302545.7460283/94‑1 (MQ=60)
                         CTCACCATCGTAAACTGACGGTGACTTTTGAGGCTGGTCAGGTATTTAAGATCCGCTTCGATCAGGGGGTGGGCTATTGGCGCATCAACTTTTA                                                                      >  SRR4302545.3073900/1‑94 (MQ=60)
                         CTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTC                                                                      <  SRR4302545.6014400/94‑1 (MQ=60)
                         CTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTC                                                                      >  SRR4302545.10545720/1‑94 (MQ=60)
                          TCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCA                                                                     <  SRR4302545.4056951/94‑1 (MQ=60)
                           CACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCAT                                                                    <  SRR4302545.111157/94‑1 (MQ=60)
                           CACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCAT                                                                    <  SRR4302545.5760161/94‑1 (MQ=60)
                           CACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCAT                                                                    <  SRR4302545.8466723/94‑1 (MQ=60)
                            ACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATC                                                                   >  SRR4302545.1848195/1‑94 (MQ=60)
                            ACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAAATTTTCATC                                                                   >  SRR4302545.2016580/1‑94 (MQ=60)
                             CCATCGTATTCTGACGGTGACTTTTGAGGATGGTCAGGTGTTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAAaatttcatca                                                                  <  SRR4302545.9646945/94‑11 (MQ=60)
                              CATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGC                                                                 >  SRR4302545.2625654/1‑94 (MQ=60)
                              CATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGC                                                                 <  SRR4302545.9474057/94‑1 (MQ=60)
                              CATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGC                                                                 >  SRR4302545.1931410/1‑94 (MQ=60)
                              CATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGC                                                                 <  SRR4302545.5905484/94‑1 (MQ=60)
                              CATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGC                                                                 <  SRR4302545.6745916/94‑1 (MQ=60)
                               ATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCA                                                                >  SRR4302545.10589583/1‑94 (MQ=60)
                               ATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCA                                                                >  SRR4302545.4265471/1‑94 (MQ=60)
                                TCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAA                                                               >  SRR4302545.10195477/1‑94 (MQ=60)
                                TCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAA                                                               >  SRR4302545.7653447/1‑94 (MQ=60)
                                 CGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAAT                                                              >  SRR4302545.8440194/1‑94 (MQ=60)
                                 CGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAAT                                                              >  SRR4302545.8440193/1‑94 (MQ=60)
                                  GTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGTCTACTGGCGCATCAACTTTTCATCGCAATG                                                             <  SRR4302545.1032618/94‑1 (MQ=60)
                                  GTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATG                                                             >  SRR4302545.6981200/1‑94 (MQ=60)
                                  GTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATG                                                             <  SRR4302545.6024103/94‑1 (MQ=60)
                                  GTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATG                                                             <  SRR4302545.3843241/94‑1 (MQ=60)
                                  GTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAAGGGGCGGGCTACTGGCCCATAAACTTTTCATCGCAATG                                                             >  SRR4302545.4987919/1‑94 (MQ=60)
                                   TAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGG                                                            <  SRR4302545.9524084/94‑1 (MQ=60)
                                   TAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGG                                                            >  SRR4302545.6675487/1‑94 (MQ=60)
                                    AAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGC                                                           >  SRR4302545.2638013/1‑94 (MQ=60)
                                    AAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGC                                                           >  SRR4302545.5079072/1‑94 (MQ=60)
                                    AAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGC                                                           >  SRR4302545.10706626/1‑94 (MQ=60)
                                     AACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCA                                                          <  SRR4302545.369993/94‑1 (MQ=60)
                                     AACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCA                                                          <  SRR4302545.64633/94‑1 (MQ=60)
                                      ACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCAT                                                         <  SRR4302545.4636806/94‑1 (MQ=60)
                                      ACTGACGGTGACTTTTGAGGATGGTCAGGGATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCAT                                                         >  SRR4302545.115755/1‑94 (MQ=60)
                                      ACTGACGGTGACTTTTGAGGATGGGCAGGGATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGAAATGGCAT                                                         >  SRR4302545.3931553/1‑94 (MQ=60)
                                       CTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATT                                                        >  SRR4302545.6226213/1‑94 (MQ=60)
                                       CTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATT                                                        >  SRR4302545.9715702/1‑94 (MQ=60)
                                       CTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATT                                                        >  SRR4302545.9059250/1‑94 (MQ=60)
                                       CTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATT                                                        >  SRR4302545.3489276/1‑94 (MQ=60)
                                        TGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTA                                                       >  SRR4302545.8962354/1‑94 (MQ=60)
                                         GACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTAC                                                      <  SRR4302545.6402360/94‑1 (MQ=60)
                                         GACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTAC                                                      <  SRR4302545.2570060/94‑1 (MQ=60)
                                         GACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTAC                                                      <  SRR4302545.6402359/94‑1 (MQ=60)
                                          ACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCAGTACT                                                     >  SRR4302545.558926/1‑94 (MQ=60)
                                           CGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTT                                                    >  SRR4302545.9431981/1‑94 (MQ=60)
                                           CGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTT                                                    >  SRR4302545.6658637/1‑94 (MQ=60)
                                           CGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTT                                                    >  SRR4302545.6377651/1‑94 (MQ=60)
                                           CGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTT                                                    >  SRR4302545.6755902/1‑94 (MQ=60)
                                            GGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTT                                                   <  SRR4302545.7154321/94‑1 (MQ=60)
                                            GGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTT                                                   >  SRR4302545.7671072/1‑94 (MQ=60)
                                            GGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTT                                                   <  SRR4302545.10422224/94‑1 (MQ=60)
                                            GGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTT                                                   >  SRR4302545.7856578/1‑94 (MQ=60)
                                            GGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTT                                                   <  SRR4302545.10378819/94‑1 (MQ=60)
                                             GTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTG                                                  <  SRR4302545.8217686/94‑1 (MQ=60)
                                             GTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTG                                                  <  SRR4302545.4613980/94‑1 (MQ=60)
                                              TGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGT                                                 >  SRR4302545.8271031/1‑94 (MQ=60)
                                              TGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGA                                                 >  SRR4302545.4636702/1‑94 (MQ=60)
                                               GACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGAT                                                >  SRR4302545.638222/1‑94 (MQ=60)
                                               GACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGAT                                                <  SRR4302545.4425893/94‑1 (MQ=60)
                                               GACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGAT                                                <  SRR4302545.4398280/94‑1 (MQ=60)
                                               GACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGAT                                                >  SRR4302545.1279839/1‑94 (MQ=60)
                                                ACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATT                                               <  SRR4302545.9009554/94‑1 (MQ=60)
                                                ACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATT                                               >  SRR4302545.1845517/1‑94 (MQ=60)
                                                ACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATT                                               <  SRR4302545.5112904/94‑1 (MQ=60)
                                                ACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATT                                               >  SRR4302545.3445135/1‑94 (MQ=60)
                                                 CTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTT                                              >  SRR4302545.3775393/1‑94 (MQ=60)
                                                   TTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCC                                            >  SRR4302545.3946647/1‑94 (MQ=60)
                                                   TTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGT‑GGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCG                                           <  SRR4302545.62466/94‑1 (MQ=60)
                                                   TTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCC                                            <  SRR4302545.9257052/94‑1 (MQ=60)
                                                    TTGAGGATGGTCAGTTATTGAAGACCGGCTTCGATAAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCG                                           <  SRR4302545.8076631/94‑1 (MQ=60)
                                                    TTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCATCTTTTCATCGCAATGGCATTACTTTGATTTCCG                                           <  SRR4302545.1106804/94‑1 (MQ=60)
                                                    TTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCG                                           <  SRR4302545.8076632/94‑1 (MQ=60)
                                                    TTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCG                                           >  SRR4302545.5746703/1‑94 (MQ=60)
                                                    TTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCG                                           <  SRR4302545.9044692/94‑1 (MQ=60)
                                                    TTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCG                                           <  SRR4302545.1822332/94‑1 (MQ=60)
                                                    TTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCG                                           <  SRR4302545.137688/94‑1 (MQ=60)
                                                     TGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGC                                          <  SRR4302545.9844717/94‑1 (MQ=60)
                                                      GAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCG                                         <  SRR4302545.5634900/94‑1 (MQ=60)
                                                      GAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCG                                         >  SRR4302545.6659415/1‑94 (MQ=60)
                                                      GAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCG                                         <  SRR4302545.3997862/94‑1 (MQ=60)
                                                       AGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGA                                        >  SRR4302545.3001143/1‑94 (MQ=60)
                                                       AGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGA                                        >  SRR4302545.6968909/1‑94 (MQ=60)
                                                       AGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGA                                        >  SRR4302545.6968908/1‑94 (MQ=60)
                                                        GGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGAT                                       <  SRR4302545.1329577/94‑1 (MQ=60)
                                                        GGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGAT                                       >  SRR4302545.2152582/1‑94 (MQ=60)
                                                        GGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGAT                                       <  SRR4302545.3630916/94‑1 (MQ=60)
                                                        GGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGAT                                       >  SRR4302545.4263474/1‑94 (MQ=60)
                                                          ATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGA                                     >  SRR4302545.5892634/1‑94 (MQ=60)
                                                           TGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGAC                                    >  SRR4302545.6918746/1‑94 (MQ=60)
                                                             GTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGT                                  <  SRR4302545.9510902/94‑1 (MQ=60)
                                                             GTCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGT                                  >  SRR4302545.5093669/1‑94 (MQ=60)
                                                              TCAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTT                                 <  SRR4302545.7331610/94‑1 (MQ=60)
                                                               CAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTT                                <  SRR4302545.3196466/94‑1 (MQ=60)
                                                               CAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTT                                >  SRR4302545.6864582/1‑94 (MQ=60)
                                                               CAGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTT                                >  SRR4302545.5689006/1‑94 (MQ=60)
                                                                AGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTC                               >  SRR4302545.1511428/1‑94 (MQ=60)
                                                                AGGTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTC                               >  SRR4302545.3445362/1‑94 (MQ=60)
                                                                  GTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCTGCGATGACGTTTCTT                             >  SRR4302545.3579639/1‑94 (MQ=60)
                                                                  GTATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTT                             >  SRR4302545.9469743/1‑94 (MQ=60)
                                                                   TATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTT                            <  SRR4302545.10589662/94‑1 (MQ=60)
                                                                   TATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTT                            >  SRR4302545.5592710/1‑94 (MQ=60)
                                                                    ATTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTC                           <  SRR4302545.6051320/94‑1 (MQ=60)
                                                                     TTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCC                          >  SRR4302545.10677548/1‑94 (MQ=60)
                                                                     TTGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCC                          <  SRR4302545.4231745/94‑1 (MQ=60)
                                                                      TGAAGATCCTCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCA                         <  SRR4302545.10441010/94‑1 (MQ=60)
                                                                      TGAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCA                         <  SRR4302545.10441009/94‑1 (MQ=60)
                                                                       GAAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAG                        >  SRR4302545.869857/1‑94 (MQ=60)
                                                                        AAGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGT                       >  SRR4302545.4279420/1‑94 (MQ=60)
                                                                         AGATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTT                      <  SRR4302545.2212964/94‑1 (MQ=60)
                                                                          GATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTA                     <  SRR4302545.7150905/94‑1 (MQ=60)
                                                                           ATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAG                    <  SRR4302545.5850971/94‑1 (MQ=60)
                                                                           ATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAG                    >  SRR4302545.6722410/1‑94 (MQ=60)
                                                                           ATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAG                    >  SRR4302545.6722411/1‑94 (MQ=60)
                                                                           ATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAG                    <  SRR4302545.10809470/94‑1 (MQ=60)
                                                                           ATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAG                    <  SRR4302545.2647476/94‑1 (MQ=60)
                                                                           ATCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAG                    <  SRR4302545.2926485/94‑1 (MQ=60)
                                                                            TCCGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGAGATGACGTTTCTTTCCAGTTAGT                   >  SRR4302545.8482661/1‑94 (MQ=60)
                                                                              CGCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCA                 <  SRR4302545.7681207/94‑1 (MQ=60)
                                                                               GCTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAA                <  SRR4302545.4235075/94‑1 (MQ=60)
                                                                                CTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAA               >  SRR4302545.8653988/1‑94 (MQ=60)
                                                                                CTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAA               >  SRR4302545.8959378/1‑94 (MQ=60)
                                                                                CTTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTCCCGCGATGACGTTTCTTTCCAGTTAGTCAAA               <  SRR4302545.8339212/94‑1 (MQ=60)
                                                                                 TTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAA              >  SRR4302545.2185946/1‑94 (MQ=60)
                                                                                 TTCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAA              >  SRR4302545.2566462/1‑94 (MQ=60)
                                                                                  TCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAAT             <  SRR4302545.8922668/94‑1 (MQ=60)
                                                                                  TCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAAT             >  SRR4302545.7158822/1‑94 (MQ=60)
                                                                                  TCGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAAT             >  SRR4302545.10307036/1‑94 (MQ=60)
                                                                                   CGATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATG            <  SRR4302545.1895028/94‑1 (MQ=60)
                                                                                     CTCAGGGGATGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGC          >  SRR4302545.6469293/1‑94 (MQ=60)
                                                                                     ATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGC          >  SRR4302545.9167481/1‑94 (MQ=60)
                                                                                     ATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGC          >  SRR4302545.9167482/1‑94 (MQ=60)
                                                                                     ATCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAAAGGCATTACGGCGAGTGCCGCGAGGACGTTTCTGTCCAGTTAGTCAAAAAGGC          >  SRR4302545.8284243/1‑94 (MQ=44)
                                                                                      TCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCT         <  SRR4302545.252126/94‑1 (MQ=60)
                                                                                      TCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCT         >  SRR4302545.3164409/1‑94 (MQ=60)
                                                                                      TCAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCT         >  SRR4302545.7891692/1‑94 (MQ=60)
                                                                                      TCAGGGGGGGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCT         <  SRR4302545.8117707/94‑1 (MQ=60)
                                                                                       CAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATTGCTC        <  SRR4302545.5708442/94‑1 (MQ=60)
                                                                                       CAGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTC        <  SRR4302545.3627544/94‑1 (MQ=60)
                                                                                        AGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCA       >  SRR4302545.1133835/1‑94 (MQ=60)
                                                                                        AGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCA       >  SRR4302545.10231765/1‑94 (MQ=60)
                                                                                        AGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCA       <  SRR4302545.470391/94‑1 (MQ=60)
                                                                                        AGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCA       >  SRR4302545.2059353/1‑94 (MQ=60)
                                                                                        AGGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCA       <  SRR4302545.6848567/94‑1 (MQ=60)
                                                                                         GGGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAG      <  SRR4302545.10032712/94‑1 (MQ=60)
                                                                                          GGGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGG     >  SRR4302545.2091860/1‑94 (MQ=60)
                                                                                           GGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGC    >  SRR4302545.2122328/1‑94 (MQ=60)
                                                                                           GGGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGC    <  SRR4302545.3494497/94‑1 (MQ=60)
                                                                                            GGTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCC   >  SRR4302545.2064353/1‑94 (MQ=60)
                                                                                             GTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCT  <  SRR4302545.8976418/94‑1 (MQ=60)
                                                                                             GTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCT  <  SRR4302545.573801/94‑1 (MQ=60)
                                                                                             GTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCT  >  SRR4302545.7342397/1‑94 (MQ=60)
                                                                                             GTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCT  >  SRR4302545.4594653/1‑94 (MQ=60)
                                                                                             GTGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCT  >  SRR4302545.5549930/1‑94 (MQ=60)
                                                                                             GTGGGCTACGGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCT  <  SRR4302545.4816097/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTGTTCGACTCGCCGCGCGATATCCCTCACCATCGTAAACTGACGGTGACTTTTGAGGATGGTCAGGTATTGAAGATCCGCTTCGATCAGGGGATGGGCTACTGGCGCATCAACTTTTCATCGCAATGGCATTACTTTGATTTCCGCGATGACGTTTCTTTCCAGTTAGTCAAAATGGCTCAGGCCT  >  NC_000913/4574165‑4574351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑