Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,614,238 G→T L133I (CTA→ATA)  yjjI ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,614,2380GT99.8% 2058.2 / ‑4.4 525L133I (CTA→ATA) yjjIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (289/235);  minor base A (0/1);  total (289/236)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 540 total aligned reads displayed.

CCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGT  >  NC_000913/4614147‑4614328
                                                                                           |                                                                                          
ccGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATaa                                                                                          >  1:384587/1‑94 (MQ=255)
 cGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAt                                                                                         >  1:10383494/1‑94 (MQ=255)
 cGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAt                                                                                         <  2:9110314/94‑1 (MQ=255)
 cGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAt                                                                                         >  1:1571907/1‑94 (MQ=255)
  gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAAtt                                                                                        <  2:8592990/94‑1 (MQ=255)
  gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATCCGAACATCGATCTCGTCTTGTCTCATAAtt                                                                                        >  2:9439957/1‑94 (MQ=255)
  gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGGTTTATTCGAACATCGATCTCGTCTTGTGTTATAAtt                                                                                        >  2:4026490/1‑94 (MQ=255)
   atatTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTc                                                                                       >  2:3597528/1‑94 (MQ=255)
    tatTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCt                                                                                      <  1:2706787/94‑1 (MQ=255)
     atTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTa                                                                                     <  2:5860026/94‑1 (MQ=255)
       tGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAAc                                                                                   <  1:3078262/94‑1 (MQ=255)
           gTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATAc                                                                               <  2:616934/94‑1 (MQ=255)
            tGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGAAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACg                                                                              <  2:10174192/94‑1 (MQ=255)
             gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACgg                                                                             <  2:5171545/94‑1 (MQ=255)
             gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGACAGAAACGTTTTATTCGAACATCGATCTCGTCTTGCGTTATAATTCTAACCTACgg                                                                             >  1:5277619/1‑94 (MQ=255)
              cATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGt                                                                            <  1:4764077/94‑1 (MQ=255)
                tAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTg                                                                          <  1:9621046/94‑1 (MQ=255)
                   aGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGcaa                                                                       <  1:2624835/94‑1 (MQ=255)
                    ggCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGcaaa                                                                      >  2:1443193/1‑93 (MQ=255)
                       gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCaacaac                                                                   <  1:7539359/94‑1 (MQ=255)
                        tCTTGCAAGTTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACg                                                                  <  2:7999856/94‑1 (MQ=255)
                          tGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCa                                                                >  2:226428/1‑94 (MQ=255)
                           ggCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCAt                                                               >  1:6844172/1‑94 (MQ=255)
                            gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATc                                                              <  2:7459850/94‑1 (MQ=255)
                              aGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCa                                                            >  2:6787891/1‑94 (MQ=255)
                              aGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCa                                                            >  1:349905/1‑94 (MQ=255)
                              aGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCa                                                            <  2:3501825/94‑1 (MQ=255)
                              aGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCa                                                            >  1:129651/1‑94 (MQ=255)
                                ggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGt                                                          >  1:2152617/1‑94 (MQ=255)
                                 gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGtt                                                         <  1:9972312/94‑1 (MQ=255)
                                    cTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCCACAACGCATCCAGTTGcc                                                      >  1:3618571/1‑94 (MQ=255)
                                    cTGTCGAGGAAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATCATTCTAACATACGGTTTCAACCACGCATCCAGTTTcc                                                      >  2:2702263/1‑94 (MQ=255)
                                     tGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGccc                                                     >  1:7649229/1‑94 (MQ=255)
                                     tGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGccc                                                     <  2:5091900/94‑1 (MQ=255)
                                        cGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAg                                                  >  1:6733916/1‑94 (MQ=255)
                                         gAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAgg                                                 >  1:1122126/1‑94 (MQ=255)
                                          aGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATCCGGTTGCAACAACGCATCCAGTTGCCCCAgga                                                >  2:8289955/1‑93 (MQ=255)
                                          aGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAggg                                                >  1:5426637/1‑93 (MQ=255)
                                          aGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGt                                                >  2:5436647/1‑94 (MQ=255)
                                             tAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGa                                             >  2:2488171/1‑94 (MQ=255)
                                              aaCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAc                                            <  2:4535308/94‑1 (MQ=255)
                                               aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAcc                                           <  1:7186451/94‑1 (MQ=255)
                                               aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAcc                                           >  1:4211434/1‑94 (MQ=255)
                                                 gCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCgg                                         >  2:4742194/1‑94 (MQ=255)
                                                 gCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCgg                                         >  1:8980163/1‑94 (MQ=255)
                                                  ccAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGc                                        >  1:6977420/1‑94 (MQ=255)
                                                  ccAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGc                                        <  2:8432333/94‑1 (MQ=255)
                                                  ccAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGc                                        <  1:9284537/94‑1 (MQ=255)
                                                   cAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCa                                       >  2:9730665/1‑94 (MQ=255)
                                                   cAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCa                                       <  1:8572351/94‑1 (MQ=255)
                                                   cAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCa                                       >  1:3372779/1‑94 (MQ=255)
                                                    aGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCAt                                      >  1:10039451/1‑94 (MQ=255)
                                                     gAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATc                                     >  1:5596825/1‑94 (MQ=255)
                                                      aaaCGTTTTATTCTAACATCGATCTCTTCTTGTGTTATAATTCTAGCATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCg                                    <  2:2003916/94‑1 (MQ=255)
                                                        aCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAt                                  >  2:7826007/1‑94 (MQ=255)
                                                        aCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAt                                  >  2:3848476/1‑94 (MQ=255)
                                                         cGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtg                                 <  2:10451046/94‑1 (MQ=255)
                                                          gTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgt                                >  2:6089255/1‑94 (MQ=255)
                                                          gTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgt                                >  2:3671561/1‑94 (MQ=255)
                                                            tttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGa                              <  1:6540300/94‑1 (MQ=255)
                                                             ttATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAc                             >  1:5733158/1‑94 (MQ=255)
                                                              tATTCGAACATCGATCTCGTCTTGTGTTAAAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGAcc                            <  1:7878244/94‑1 (MQ=255)
                                                                 tCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGAc                         >  1:7464977/1‑94 (MQ=255)
                                                                 tCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGAc                         <  2:2349108/94‑1 (MQ=255)
                                                                 tCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGAc                         >  1:5076841/1‑94 (MQ=255)
                                                                  cGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACg                        <  2:5610490/94‑1 (MQ=255)
                                                                   gAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGAGCGGCATCGATGTGACCGACgg                       >  1:6244507/1‑94 (MQ=255)
                                                                   gAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACgg                       <  2:1254511/94‑1 (MQ=255)
                                                                    aaCATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGTCCCAGGTAGACCGGCATCGATGTGACCGACgga                      >  2:714941/1‑93 (MQ=255)
                                                                      cATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTAc                    <  1:6353667/94‑1 (MQ=255)
                                                                       aTCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                   >  1:619683/1‑94 (MQ=255)
                                                                       aTCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                   >  2:4310729/1‑94 (MQ=255)
                                                                       aTCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACg                   >  2:4837728/1‑94 (MQ=255)
                                                                         ttaTCTCGTGTTGGTTTATAATTCTAACAGACGGTTCCAACAACGCGTCCAGTTGCTCCAGGTAGACCGGCATCGATGTGCCCGACGGTACgtg                 <  1:8679422/92‑1 (MQ=255)
                                                                         cGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                 <  2:8075880/94‑1 (MQ=255)
                                                                         cGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                 <  1:727567/94‑1 (MQ=255)
                                                                         cGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtg                 >  2:655173/1‑94 (MQ=255)
                                                                          gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg                <  1:1740316/94‑1 (MQ=255)
                                                                          gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg                <  2:4273369/94‑1 (MQ=255)
                                                                          gATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg                >  1:2990930/1‑94 (MQ=255)
                                                                           aTCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggg               >  1:4440111/1‑93 (MQ=255)
                                                                            tctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtggtg              >  2:2880357/1‑94 (MQ=255)
                                                                             ctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGtggtgg             >  2:9306673/1‑94 (MQ=255)
                                                                             ctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGtggtgg             <  1:10235489/94‑1 (MQ=255)
                                                                             ctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGtggtgg             >  2:10294346/1‑94 (MQ=255)
                                                                             ctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGtggtgg             <  2:7026647/94‑1 (MQ=255)
                                                                             ctcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGtggtgg             >  1:4628924/1‑94 (MQ=255)
                                                                              tcGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTggtggt            >  1:1463294/1‑94 (MQ=255)
                                                                               cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTa           <  2:1710295/94‑1 (MQ=255)
                                                                                  cTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAg        >  2:697085/1‑94 (MQ=255)
                                                                                  cTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAg        >  1:3917904/1‑94 (MQ=255)
                                                                                  cTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAg        <  2:3206845/94‑1 (MQ=255)
                                                                                     gtgtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAAt     >  2:6378106/1‑94 (MQ=255)
                                                                                      tgtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATg    >  1:538334/1‑94 (MQ=255)
                                                                                      tgtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATg    >  1:2007765/1‑94 (MQ=255)
                                                                                      tgtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATg    >  1:5925314/1‑94 (MQ=255)
                                                                                       gtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATgg   >  1:2376792/1‑94 (MQ=255)
                                                                                       gtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATgg   >  2:9058252/1‑94 (MQ=255)
                                                                                       gtTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATgg   >  1:8047729/1‑94 (MQ=255)
                                                                                        ttATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGt  <  1:8356514/94‑1 (MQ=255)
                                                                                           |                                                                                          
CCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGT  >  NC_000913/4614147‑4614328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 308 total aligned reads displayed.
TTATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGG  >  NC_000913/4620347‑4620532
                                                                                             |                                                                                            
TTATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGAC                                                                                              >  SRR4302545.501176/1‑94 (MQ=60)
TTATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGAC                                                                                              <  SRR4302545.3066269/94‑1 (MQ=60)
TTATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGAC                                                                                              >  SRR4302545.5793535/1‑94 (MQ=60)
 TATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACG                                                                                             >  SRR4302545.10275437/1‑94 (MQ=60)
 TATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACG                                                                                             >  SRR4302545.10813283/1‑94 (MQ=60)
 TATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACG                                                                                             >  SRR4302545.1103479/1‑94 (MQ=60)
  ATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGG                                                                                            >  SRR4302545.4559530/1‑94 (MQ=60)
   TCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGT                                                                                           <  SRR4302545.8677384/94‑1 (MQ=60)
   TCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGT                                                                                           <  SRR4302545.8589238/94‑1 (MQ=60)
   TCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGT                                                                                           <  SRR4302545.578788/94‑1 (MQ=60)
    CGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTC                                                                                          <  SRR4302545.9818502/94‑1 (MQ=60)
     GCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGACTACGACATGCTGCCGACGGTCT                                                                                         >  SRR4302545.5736003/1‑94 (MQ=60)
      CCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTA                                                                                        <  SRR4302545.2612545/94‑1 (MQ=60)
      CCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTA                                                                                        <  SRR4302545.9799928/94‑1 (MQ=60)
      CCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTA                                                                                        <  SRR4302545.4173233/94‑1 (MQ=60)
       CAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTAT                                                                                       >  SRR4302545.9090933/1‑94 (MQ=60)
       CAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTAT                                                                                       >  SRR4302545.8100618/1‑94 (MQ=60)
        AACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATG                                                                                      >  SRR4302545.7285426/1‑94 (MQ=60)
        AACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATG                                                                                      <  SRR4302545.3804937/94‑1 (MQ=60)
        AACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCAACGGTCTATG                                                                                      >  SRR4302545.20876/1‑94 (MQ=60)
          CAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCA                                                                                    <  SRR4302545.5477082/94‑1 (MQ=60)
          CAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCA                                                                                    >  SRR4302545.5469280/1‑94 (MQ=60)
          CAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCA                                                                                    >  SRR4302545.8813733/1‑94 (MQ=60)
          CAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCA                                                                                    >  SRR4302545.9174486/1‑94 (MQ=60)
          CAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCA                                                                                    <  SRR4302545.3692968/94‑1 (MQ=60)
           AGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCAC                                                                                   >  SRR4302545.6001982/1‑94 (MQ=60)
             CGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCA                                                                                 <  SRR4302545.288054/94‑1 (MQ=60)
              GATATGCACGCAGGTAATTTATCGGTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAA                                                                                <  SRR4302545.1806792/94‑1 (MQ=60)
               ATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAA                                                                               >  SRR4302545.10330976/1‑94 (MQ=60)
               ATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAA                                                                               >  SRR4302545.3151400/1‑94 (MQ=60)
                TATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAAC                                                                              <  SRR4302545.7480702/94‑1 (MQ=60)
                 ATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTGCGACATGCTGCCGACGGTCTATGCACCAAACA                                                                             <  SRR4302545.2864140/94‑1 (MQ=60)
                 ATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACA                                                                             >  SRR4302545.3052879/1‑94 (MQ=60)
                 ATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACA                                                                             <  SRR4302545.2864139/94‑1 (MQ=60)
                  TGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAG                                                                            >  SRR4302545.3444908/1‑94 (MQ=60)
                  TGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAG                                                                            <  SRR4302545.7934506/94‑1 (MQ=60)
                   GCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGC                                                                           <  SRR4302545.2127780/94‑1 (MQ=60)
                    CACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCG                                                                          <  SRR4302545.8741248/94‑1 (MQ=60)
                    CACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCG                                                                          >  SRR4302545.10620739/1‑94 (MQ=60)
                    CACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCG                                                                          <  SRR4302545.151704/94‑1 (MQ=60)
                    CACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCG                                                                          >  SRR4302545.10145877/1‑94 (MQ=60)
                     ACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGC                                                                         <  SRR4302545.5430430/94‑1 (MQ=60)
                     ACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGC                                                                         >  SRR4302545.10263564/1‑94 (MQ=60)
                      CGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCT                                                                        >  SRR4302545.9870958/1‑94 (MQ=60)
                      CGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCT                                                                        >  SRR4302545.8192663/1‑94 (MQ=60)
                      CGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCT                                                                        >  SRR4302545.4943461/1‑94 (MQ=60)
                      CGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCT                                                                        >  SRR4302545.8192662/1‑94 (MQ=60)
                       GCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTG                                                                       >  SRR4302545.7218345/1‑94 (MQ=60)
                       GCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTA                                                                       <  SRR4302545.330174/94‑1 (MQ=60)
                        CATGTAAGTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTGCGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTTG                                                                      <  SRR4302545.1726440/94‑1 (MQ=60)
                        CAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGG                                                                      <  SRR4302545.8772108/94‑1 (MQ=60)
                        CAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGG                                                                      >  SRR4302545.4175596/1‑94 (MQ=60)
                        CAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGG                                                                      <  SRR4302545.9149175/94‑1 (MQ=60)
                        CAGGTAATGTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGG                                                                      <  SRR4302545.9149176/94‑1 (MQ=60)
                         AGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGA                                                                     >  SRR4302545.1789884/1‑94 (MQ=60)
                         AGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGA                                                                     <  SRR4302545.8009047/94‑1 (MQ=60)
                         AGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGA                                                                     <  SRR4302545.2782235/94‑1 (MQ=60)
                         AGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGA                                                                     >  SRR4302545.7197758/1‑94 (MQ=60)
                          GGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAA                                                                    >  SRR4302545.4226697/1‑94 (MQ=60)
                          GGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAA                                                                    >  SRR4302545.4976208/1‑94 (MQ=60)
                            TAATTTATCGTTTTATTTATCTGAAGCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATG                                                                  >  SRR4302545.10446795/1‑94 (MQ=60)
                            TAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATG                                                                  >  SRR4302545.5381229/1‑94 (MQ=60)
                            TAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATG                                                                  >  SRR4302545.3928218/1‑94 (MQ=60)
                            TAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATG                                                                  <  SRR4302545.5075333/94‑1 (MQ=60)
                            TAATTTATCGTTTAATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATG                                                                  <  SRR4302545.6700221/94‑1 (MQ=60)
                            TAATTTATCGTTTAATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATG                                                                  <  SRR4302545.6700222/94‑1 (MQ=60)
                             AATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGC                                                                 >  SRR4302545.10637027/1‑94 (MQ=60)
                             AATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGC                                                                 <  SRR4302545.4348128/94‑1 (MQ=60)
                              ATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCT                                                                <  SRR4302545.3057673/94‑1 (MQ=60)
                              ATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCT                                                                <  SRR4302545.5612312/94‑1 (MQ=60)
                              ATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCG                                                                >  SRR4302545.5357742/1‑94 (MQ=60)
                               tccgatctctgatcTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTG                                                               <  SRR4302545.3433539/80‑1 (MQ=60)
                                TTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGC                                                              >  SRR4302545.2415780/1‑94 (MQ=60)
                                TTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGC                                                              <  SRR4302545.7909346/94‑1 (MQ=60)
                                 TATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCG                                                             >  SRR4302545.6090073/1‑94 (MQ=60)
                                 TATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCG                                                             <  SRR4302545.6740793/94‑1 (MQ=60)
                                  ATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCACACAGCGCTGGAATGCTGCGT                                                            >  SRR4302545.7540149/1‑94 (MQ=60)
                                  ATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGT                                                            <  SRR4302545.1005531/94‑1 (MQ=60)
                                  ATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGT                                                            >  SRR4302545.4862665/1‑94 (MQ=60)
                                  ATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGT                                                            >  SRR4302545.5602079/1‑94 (MQ=60)
                                  ATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGGCTATGCACCAAACAGCGCTGGAATGCTGCGG                                                            >  SRR4302545.9268964/1‑94 (MQ=60)
                                   TCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTG                                                           <  SRR4302545.1160051/94‑1 (MQ=60)
                                    CGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGA                                                          >  SRR4302545.8971814/1‑94 (MQ=60)
                                    CGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCCAACAGCGCTGGAATGCTGCGTGA                                                          >  SRR4302545.6004019/1‑94 (MQ=60)
                                     GTTTTATTTATCTGACCCGCCATTTGAGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGAACCAAACAGCGCTGGAATGCTGCGTGAT                                                         <  SRR4302545.6841507/94‑1 (MQ=60)
                                     GTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGAT                                                         >  SRR4302545.1680088/1‑94 (MQ=60)
                                     GTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGAT                                                         >  SRR4302545.3607568/1‑94 (MQ=60)
                                      TTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATG                                                        >  SRR4302545.10885345/1‑94 (MQ=60)
                                        TTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCT                                                      <  SRR4302545.2083754/94‑1 (MQ=60)
                                         TATTTATCTGAACCGCCATTTGCGCTGGCGCCCGTCGACGACTTGCTGCCGACGGTCTATTCACCAAACAGCGCTTGTATGGTGCGTGACGCTG                                                     >  SRR4302545.6251830/1‑94 (MQ=55)
                                         TATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGCCGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTG                                                     <  SRR4302545.10694331/94‑1 (MQ=60)
                                         TATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTG                                                     <  SRR4302545.8100815/94‑1 (MQ=60)
                                          ATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGC                                                    >  SRR4302545.1392969/1‑94 (MQ=60)
                                          ATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGC                                                    >  SRR4302545.6425744/1‑94 (MQ=60)
                                           TTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCC                                                   >  SRR4302545.366182/1‑94 (MQ=60)
                                            TTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCA                                                  <  SRR4302545.652032/94‑1 (MQ=60)
                                            TTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCA                                                  >  SRR4302545.9955976/1‑94 (MQ=60)
                                            TTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCA                                                  <  SRR4302545.4222801/94‑1 (MQ=60)
                                            TTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCA                                                  <  SRR4302545.903414/94‑1 (MQ=60)
                                             TATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCAT                                                 <  SRR4302545.5903715/94‑1 (MQ=60)
                                              ATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATT                                                <  SRR4302545.7908014/94‑1 (MQ=60)
                                              ATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATT                                                <  SRR4302545.9150915/94‑1 (MQ=60)
                                              ATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATT                                                <  SRR4302545.9150916/94‑1 (MQ=60)
                                              ATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATT                                                <  SRR4302545.4334126/94‑1 (MQ=60)
                                                CTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGA                                              >  SRR4302545.203755/1‑94 (MQ=60)
                                                 TGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAG                                             >  SRR4302545.10506890/1‑94 (MQ=60)
                                                 TGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAG                                             >  SRR4302545.6787376/1‑94 (MQ=60)
                                                 TGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAG                                             >  SRR4302545.228780/1‑94 (MQ=60)
                                                  GAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGG                                            >  SRR4302545.4765331/1‑94 (MQ=60)
                                                     CCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGGACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGA                                         >  SRR4302545.5571214/1‑94 (MQ=60)
                                                     CCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGA                                         >  SRR4302545.6792754/1‑94 (MQ=60)
                                                     CCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGA                                         <  SRR4302545.8946326/94‑1 (MQ=60)
                                                      CGCCATTTGCGGTGACGCCAGTCTACGACATGCTGCCGACGGCCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGCGAA                                        >  SRR4302545.7069795/1‑94 (MQ=60)
                                                      CGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAA                                        >  SRR4302545.2897028/1‑94 (MQ=60)
                                                      CGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAA                                        >  SRR4302545.8342943/1‑94 (MQ=60)
                                                       GCCATTTGCGCTGACGCGCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAG                                       >  SRR4302545.10331363/1‑94 (MQ=60)
                                                       GCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAG                                       >  SRR4302545.3549568/1‑94 (MQ=60)
                                                       GCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAG                                       >  SRR4302545.3927684/1‑94 (MQ=60)
                                                        CCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGT                                      <  SRR4302545.6414991/94‑1 (MQ=60)
                                                        CCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGT                                      >  SRR4302545.3596284/1‑94 (MQ=60)
                                                        CCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGCGATGCTGCCATTGAGGGGAAGT                                      >  SRR4302545.3876304/1‑94 (MQ=60)
                                                         CATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTT                                     >  SRR4302545.2174541/1‑94 (MQ=60)
                                                         CATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTT                                     >  SRR4302545.7037781/1‑94 (MQ=60)
                                                          ATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTT                                    >  SRR4302545.6096974/1‑94 (MQ=60)
                                                          ATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTT                                    <  SRR4302545.4579672/94‑1 (MQ=60)
                                                          ATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTT                                    >  SRR4302545.9311261/1‑94 (MQ=60)
                                                          ATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTT                                    >  SRR4302545.10550221/1‑94 (MQ=60)
                                                          ATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTT                                    >  SRR4302545.6129770/1‑94 (MQ=60)
                                                          ATTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTT                                    >  SRR4302545.5195428/1‑94 (MQ=60)
                                                           TTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTG                                   <  SRR4302545.1897869/94‑1 (MQ=60)
                                                           TTTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTG                                   <  SRR4302545.1897868/94‑1 (MQ=60)
                                                            TTGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGA                                  <  SRR4302545.5970967/94‑1 (MQ=60)
                                                            TTGCGCTGACCCCCGTCTACGACATGCTGGCGACGGTCGATGCACCAAACAGCTCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGA                                  <  SRR4302545.5188924/94‑1 (MQ=60)
                                                             TGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGAT                                 <  SRR4302545.6569877/94‑1 (MQ=60)
                                                             TGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGAT                                 >  SRR4302545.10412452/1‑94 (MQ=60)
                                                             TGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGAT                                 <  SRR4302545.7536066/94‑1 (MQ=60)
                                                             TGCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGAT                                 <  SRR4302545.10926954/94‑1 (MQ=60)
                                                              GCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATC                                <  SRR4302545.967955/94‑1 (MQ=60)
                                                              GCGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATC                                <  SRR4302545.8879571/94‑1 (MQ=60)
                                                               CGCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCT                               >  SRR4302545.2481095/1‑94 (MQ=60)
                                                                GCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTT                              >  SRR4302545.1824435/1‑94 (MQ=60)
                                                                GCTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGCGATGCTGCCATTGAGGTGAAGTTTGATCTT                              >  SRR4302545.6361502/1‑94 (MQ=60)
                                                                 CTGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTA                             >  SRR4302545.5339837/1‑94 (MQ=60)
                                                                  TGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAA                            <  SRR4302545.2441721/94‑1 (MQ=60)
                                                                  TGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAA                            <  SRR4302545.6954912/94‑1 (MQ=60)
                                                                  TGACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAA                            >  SRR4302545.6624467/1‑94 (MQ=60)
                                                                   GACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAAC                           <  SRR4302545.10143127/94‑1 (MQ=60)
                                                                   GACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAAC                           <  SRR4302545.7475073/94‑1 (MQ=60)
                                                                    ACGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACG                          >  SRR4302545.7018744/1‑94 (MQ=60)
                                                                     CGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGT                         >  SRR4302545.4069766/1‑94 (MQ=60)
                                                                     CGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGT                         <  SRR4302545.2321962/94‑1 (MQ=60)
                                                                     CGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGT                         <  SRR4302545.2957332/94‑1 (MQ=60)
                                                                     CGCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGT                         >  SRR4302545.5413417/1‑94 (MQ=60)
                                                                      GCCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTC                        >  SRR4302545.4448188/1‑94 (MQ=60)
                                                                       CCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCA                       <  SRR4302545.6768048/94‑1 (MQ=60)
                                                                       CCCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCA                       <  SRR4302545.6768047/94‑1 (MQ=60)
                                                                        CCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAG                      >  SRR4302545.9469839/1‑94 (MQ=60)
                                                                        CCGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAG                      <  SRR4302545.770327/94‑1 (MQ=60)
                                                                         CGTCTACGACATGCTGCCGACGGTCTATGCACCAATCAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGT                     <  SRR4302545.1822864/94‑1 (MQ=60)
                                                                         CGTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGT                     >  SRR4302545.10038161/1‑94 (MQ=60)
                                                                          GTCTACGTCTTGCTGCCGACGGTCTATGCACCAAACAGCGTTGGAATGCTGCGTGttgttgtcattgaggcgatttttgatcttttctttagta                    >  SRR4302545.9992646/1‑55 (MQ=40)
                                                                          GTCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTA                    <  SRR4302545.10838201/94‑1 (MQ=60)
                                                                           TCTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAA                   <  SRR4302545.5570464/94‑1 (MQ=60)
                                                                            CTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAA                  >  SRR4302545.1233163/1‑94 (MQ=60)
                                                                            CTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAA                  >  SRR4302545.1602333/1‑94 (MQ=60)
                                                                            CTACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAA                  >  SRR4302545.1337065/1‑94 (MQ=60)
                                                                             TACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAA                 <  SRR4302545.9001514/94‑1 (MQ=60)
                                                                             TACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAA                 <  SRR4302545.6266759/94‑1 (MQ=60)
                                                                              ACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAG                <  SRR4302545.807695/94‑1 (MQ=60)
                                                                              ACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAG                >  SRR4302545.3582314/1‑94 (MQ=60)
                                                                              ACGACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAG                <  SRR4302545.9841951/94‑1 (MQ=60)
                                                                                GACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGCTGCTGCCATTTAGGGGAAGTTTGATCTTAACGACAGTAAAAGCG              >  SRR4302545.6749555/1‑94 (MQ=60)
                                                                                GACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCG              >  SRR4302545.9718636/1‑94 (MQ=60)
                                                                                GACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCG              <  SRR4302545.9524808/94‑1 (MQ=60)
                                                                                GACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCG              <  SRR4302545.9524807/94‑1 (MQ=60)
                                                                                GACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCG              >  SRR4302545.10500281/1‑94 (MQ=60)
                                                                                 ACATGCTGCCGACGTGCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGC             <  SRR4302545.7791476/94‑1 (MQ=60)
                                                                                 ACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGC             >  SRR4302545.7400620/1‑94 (MQ=60)
                                                                                 ACATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGC             >  SRR4302545.7400621/1‑94 (MQ=60)
                                                                                  CATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCT            <  SRR4302545.2375424/94‑1 (MQ=60)
                                                                                  CATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCT            <  SRR4302545.9984989/94‑1 (MQ=60)
                                                                                   ATGCTGCCGCCGGTCTATGCACCAAACAGCGCTTGCATGCTGCGTGGTGCTGTCATTGAGGCGAAGTTTGATCTTAACGTCAGTAAACGCGCTT           >  SRR4302545.7568776/1‑94 (MQ=60)
                                                                                   ATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTT           <  SRR4302545.7065229/94‑1 (MQ=60)
                                                                                   ATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTT           >  SRR4302545.3817317/1‑94 (MQ=60)
                                                                                   ATGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTT           >  SRR4302545.2363206/1‑94 (MQ=60)
                                                                                    TGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTG          <  SRR4302545.8996789/94‑1 (MQ=60)
                                                                                    TGCTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTG          <  SRR4302545.7967265/94‑1 (MQ=60)
                                                                                      CTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGT        >  SRR4302545.3360826/1‑94 (MQ=60)
                                                                                      CTGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCAGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGT        >  SRR4302545.1645033/1‑94 (MQ=60)
                                                                                       TGCCGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTT       <  SRR4302545.7924458/94‑1 (MQ=60)
                                                                                          CGACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAAC    >  SRR4302545.7780447/1‑94 (MQ=60)
                                                                                           GACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACG   >  SRR4302545.4068038/1‑94 (MQ=60)
                                                                                           GACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACG   <  SRR4302545.5260719/94‑1 (MQ=60)
                                                                                           GACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACG   <  SRR4302545.5260721/94‑1 (MQ=60)
                                                                                           GACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACG   <  SRR4302545.9306493/94‑1 (MQ=60)
                                                                                           GACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACG   >  SRR4302545.10855873/1‑94 (MQ=60)
                                                                                           GACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACG   <  SRR4302545.10710675/94‑1 (MQ=60)
                                                                                            ACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTGACGTCAGTAAAAGCGCTTGGTTAACGG  >  SRR4302545.769769/1‑94 (MQ=60)
                                                                                            ACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGG  <  SRR4302545.2792725/94‑1 (MQ=60)
                                                                                            ACGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGG  <  SRR4302545.2731331/94‑1 (MQ=60)
                                                                                             |                                                                                            
TTATCGCCAACAGCGATATGCACGCAGGTAATTTATCGTTTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGG  >  NC_000913/4620347‑4620532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑