Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGC  >  NC_000913/2072092‑2072274
                                                                                          |                                                                                            
ACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTG                                                                                           >  SRR4302545.9260509/1‑94 (MQ=60)
 CAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGC                                                                                          >  SRR4302545.8675736/1‑94 (MQ=60)
 CAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGC                                                                                          >  SRR4302545.7918827/1‑94 (MQ=60)
   GGAAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCC                                                                                        <  SRR4302545.3207565/94‑1 (MQ=60)
    GAAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCT                                                                                       <  SRR4302545.3961853/94‑1 (MQ=60)
          CGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTA                                                                                 >  SRR4302545.538893/1‑94 (MQ=60)
           GCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTAT                                                                                >  SRR4302545.10353732/1‑94 (MQ=60)
           GCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTAT                                                                                >  SRR4302545.2192180/1‑94 (MQ=60)
                AGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTG                                                                           >  SRR4302545.1080283/1‑94 (MQ=60)
                    TGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAA                                                                       <  SRR4302545.4857385/94‑1 (MQ=60)
                    TGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAA                                                                       <  SRR4302545.4857384/94‑1 (MQ=60)
                    TGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAA                                                                       >  SRR4302545.7686240/1‑94 (MQ=60)
                     GAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAAC                                                                      >  SRR4302545.2182672/1‑94 (MQ=60)
                      AAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACC                                                                     >  SRR4302545.4555494/1‑94 (MQ=60)
                      AAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACC                                                                     <  SRR4302545.6518172/94‑1 (MQ=60)
                       AAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCT                                                                    >  SRR4302545.494187/1‑94 (MQ=60)
                          CGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGAT                                                                 >  SRR4302545.2940090/1‑94 (MQ=60)
                           GTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATG                                                                >  SRR4302545.5789573/1‑94 (MQ=60)
                           GTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATG                                                                >  SRR4302545.6463421/1‑94 (MQ=60)
                           GTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATG                                                                <  SRR4302545.2904211/94‑1 (MQ=60)
                            TCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGC                                                               >  SRR4302545.7885956/1‑94 (MQ=60)
                             CATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCT                                                              >  SRR4302545.6922522/1‑94 (MQ=60)
                             CATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCT                                                              >  SRR4302545.7825538/1‑94 (MQ=60)
                             CATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCT                                                              <  SRR4302545.2361189/94‑1 (MQ=60)
                             CATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCT                                                              <  SRR4302545.10170270/94‑1 (MQ=60)
                              CTCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAACCCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTG                                                             <  SRR4302545.1958140/94‑1 (MQ=60)
                              ATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTG                                                             <  SRR4302545.4414872/94‑1 (MQ=60)
                               TCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGG                                                            <  SRR4302545.6539553/94‑1 (MQ=60)
                                CGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGA                                                           <  SRR4302545.9079625/94‑1 (MQ=60)
                                 GGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAG                                                          >  SRR4302545.271287/1‑94 (MQ=60)
                                   ACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCC                                                        >  SRR4302545.7945465/1‑94 (MQ=60)
                                      ATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACA                                                     >  SRR4302545.10434764/1‑94 (MQ=60)
                                      ATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACA                                                     >  SRR4302545.4276873/1‑94 (MQ=60)
                                          cgtgttccgcgcacggagtgggggtTGGCTGGGCGCACTGGAAACCGGCCTGCCCTTAAGTATCACTGACAACCTGATGCTGGAGCTTCAACTG                                                 <  SRR4302545.3993091/69‑1 (MQ=60)
                                          CGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTG                                                 <  SRR4302545.3993089/94‑1 (MQ=60)
                                           GACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGC                                                >  SRR4302545.8330050/1‑94 (MQ=60)
                                            ACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCA                                               <  SRR4302545.317398/94‑1 (MQ=60)
                                             CTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAG                                              >  SRR4302545.5336043/1‑94 (MQ=60)
                                             CTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAG                                              >  SRR4302545.7733723/1‑94 (MQ=60)
                                              TTCCGCGCCCGGGGCGGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGT                                             <  SRR4302545.3985475/94‑1 (MQ=60)
                                              CTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGT                                             <  SRR4302545.4329159/94‑1 (MQ=60)
                                               TCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTA                                            >  SRR4302545.5678475/1‑94 (MQ=60)
                                               TCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTA                                            >  SRR4302545.9286516/1‑94 (MQ=60)
                                                CCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTAT                                           <  SRR4302545.9761186/94‑1 (MQ=60)
                                                    GCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCT                                       <  SRR4302545.2115520/94‑1 (MQ=60)
                                                    GCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCT                                       <  SRR4302545.2189795/94‑1 (MQ=60)
                                                     CCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTG                                      <  SRR4302545.640188/94‑1 (MQ=60)
                                                      CCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGG                                     >  SRR4302545.1169312/1‑94 (MQ=60)
                                                      CCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCATCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGG                                     >  SRR4302545.1169311/1‑94 (MQ=60)
                                                      CCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGG                                     <  SRR4302545.1367026/94‑1 (MQ=60)
                                                      CCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGG                                     <  SRR4302545.1582815/94‑1 (MQ=60)
                                                       CGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTctacttgga                                    >  SRR4302545.5534580/1‑85 (MQ=60)
                                                        GGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCA                                   <  SRR4302545.10423748/94‑1 (MQ=60)
                                                         GGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAG                                  <  SRR4302545.94465/94‑1 (MQ=60)
                                                          GGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGG                                 >  SRR4302545.7016506/1‑94 (MQ=60)
                                                            CTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGA                               >  SRR4302545.1501332/1‑94 (MQ=60)
                                                            CTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGA                               >  SRR4302545.1373677/1‑94 (MQ=60)
                                                            CTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGA                               >  SRR4302545.1373676/1‑94 (MQ=60)
                                                              GGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACT                             >  SRR4302545.4174903/1‑94 (MQ=60)
                                                              GGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACT                             <  SRR4302545.10881316/94‑1 (MQ=60)
                                                              GGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACT                             <  SRR4302545.4690063/94‑1 (MQ=60)
                                                              GGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACT                             <  SRR4302545.2594303/94‑1 (MQ=60)
                                                              GGGGCTGGCAGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCCGGCAGGGACT                             <  SRR4302545.7244397/94‑1 (MQ=60)
                                                                GGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTT                           <  SRR4302545.8845683/94‑1 (MQ=60)
                                                                GGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTT                           <  SRR4302545.8174011/94‑1 (MQ=60)
                                                                GGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTT                           <  SRR4302545.1931411/94‑1 (MQ=60)
                                                                GGCTGGCGGGGCTCACTGGAAACCGGCCTGCCATTCAGTATCACTGACAACCTGAGGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTT                           <  SRR4302545.1191122/94‑1 (MQ=60)
                                                                   TGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCC                        >  SRR4302545.2734429/1‑94 (MQ=60)
                                                                   TGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCC                        >  SRR4302545.2734430/1‑94 (MQ=60)
                                                                    GGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCT                       >  SRR4302545.6226234/1‑94 (MQ=60)
                                                                     GCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTG                      <  SRR4302545.7110355/94‑1 (MQ=60)
                                                                        GGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGAT                   >  SRR4302545.3365728/1‑94 (MQ=60)
                                                                           CTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGAC                <  SRR4302545.6923774/94‑1 (MQ=60)
                                                                           CTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGAC                <  SRR4302545.6923773/94‑1 (MQ=60)
                                                                            TCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACG               <  SRR4302545.316686/94‑1 (MQ=60)
                                                                             CACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGG              <  SRR4302545.6079451/94‑1 (MQ=60)
                                                                               CTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTA            >  SRR4302545.5926741/1‑94 (MQ=60)
                                                                                TGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAA           >  SRR4302545.9921787/1‑94 (MQ=60)
                                                                                TGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAA           >  SRR4302545.1409864/1‑94 (MQ=60)
                                                                                     ACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACA      <  SRR4302545.463347/94‑1 (MQ=60)
                                                                                      CCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAA     <  SRR4302545.697801/94‑1 (MQ=60)
                                                                                         GTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGC  >  SRR4302545.10283352/1‑94 (MQ=60)
                                                                                         GCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGC  >  SRR4302545.3759548/1‑94 (MQ=60)
                                                                                          |                                                                                            
ACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGC  >  NC_000913/2072092‑2072274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑