Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,072,182 T→C G874G (GGT→GGC)  b2000 → outer membrane fluffing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,072,1820TC99.5% 633.2 / NA 190G874G (GGT→GGC) b2000outer membrane fluffing protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (83/106);  total (84/106)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 200 total aligned reads displayed.

ACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAA  >  NC_000913/2072092‑2072271
                                                                                          |                                                                                         
acaGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCt                                                                                         >  2:515552/1‑93 (MQ=255)
  aGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGc                                                                                       >  1:1445908/1‑93 (MQ=255)
   gggAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGcc                                                                                      >  1:3346314/1‑93 (MQ=255)
   gggAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAAACGGCCtcgc                                                                                      >  2:4782145/1‑90 (MQ=255)
    ggACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGccc                                                                                     <  1:36895/93‑1 (MQ=255)
    ggACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGccc                                                                                     <  1:2471660/93‑1 (MQ=255)
    ggAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCCGCCTGccc                                                                                     >  1:4857843/1‑93 (MQ=255)
     gAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCt                                                                                    >  1:606811/1‑93 (MQ=255)
      accccGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCtt                                                                                   <  2:254447/91‑1 (MQ=255)
         ccGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAg                                                                                >  1:2433389/1‑93 (MQ=255)
          cGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGt                                                                               <  1:4385495/93‑1 (MQ=255)
           gCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTa                                                                              <  1:5730104/93‑1 (MQ=255)
           gCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTa                                                                              <  1:4962326/93‑1 (MQ=255)
              acaGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCa                                                                           >  1:753852/1‑93 (MQ=255)
              acaGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCa                                                                           <  2:5985153/93‑1 (MQ=255)
               caGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCAc                                                                          <  1:1909817/93‑1 (MQ=255)
               caGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCAc                                                                          >  2:1754217/1‑93 (MQ=255)
                 gCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTg                                                                        <  1:5607920/93‑1 (MQ=255)
                  cATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGa                                                                       >  2:5084062/1‑93 (MQ=255)
                  cATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGa                                                                       >  2:5483146/1‑93 (MQ=255)
                  cATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGa                                                                       <  1:5163756/93‑1 (MQ=255)
                    tGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACa                                                                     >  1:6001232/1‑93 (MQ=255)
                    tGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACa                                                                     >  1:2295812/1‑93 (MQ=255)
                      aaaGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAAc                                                                   >  2:3396801/1‑93 (MQ=255)
                      aaaGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAAc                                                                   >  2:2990694/1‑93 (MQ=255)
                       aaGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAAcc                                                                  >  1:2374332/1‑93 (MQ=255)
                          cGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGa                                                               <  1:4713929/93‑1 (MQ=255)
                           gTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGGAACCGGCCTGCCCTTCCGTATCACTGACAACCTGAt                                                              >  2:5971274/1‑93 (MQ=255)
                            tCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATg                                                             >  1:1043081/1‑93 (MQ=255)
                            tCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATg                                                             >  1:5437007/1‑93 (MQ=255)
                             cATCGGACAATAACTACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGc                                                            <  1:2806002/93‑1 (MQ=255)
                               tCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTg                                                          <  1:2954231/93‑1 (MQ=255)
                                cGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTgg                                                         <  2:6238204/93‑1 (MQ=255)
                                 tgACAATAACGACTTCCGCGCGCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACGGACAACCTGATGCTGGa                                                        <  1:5737838/92‑1 (MQ=255)
                                 ggACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGa                                                        <  2:2603913/93‑1 (MQ=255)
                                 ggACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGa                                                        <  1:3134031/93‑1 (MQ=255)
                                 ggACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGa                                                        <  1:3252661/93‑1 (MQ=255)
                                 ggACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGa                                                        >  1:4430405/1‑93 (MQ=255)
                                 ggACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGACCTTCAGTATCACTGACAAACTGATGCTGGa                                                        >  2:5223088/1‑93 (MQ=255)
                                 ggACAATAACGACTTCCGCGCCAGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGa                                                        <  1:4130207/93‑1 (MQ=255)
                                  gagaaTAACGACTTCCGCGCCCGGGGCTGGTGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTTACAACCTGATGCTGGAg                                                       <  2:4154476/90‑1 (MQ=255)
                                  gACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAg                                                       >  2:585280/1‑93 (MQ=255)
                                    cAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGcc                                                     >  2:3183858/1‑93 (MQ=255)
                                    cAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGcc                                                     >  1:2591825/1‑93 (MQ=255)
                                        aaCGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACaa                                                 >  1:6109350/1‑93 (MQ=255)
                                         aCGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAAc                                                >  2:4160090/1‑93 (MQ=255)
                                         aCGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAAc                                                >  1:6455811/1‑93 (MQ=255)
                                         aCGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAAc                                                <  2:5823753/93‑1 (MQ=255)
                                         aCGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAAc                                                >  1:5055910/1‑93 (MQ=255)
                                          cGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACt                                               <  2:643511/93‑1 (MQ=255)
                                            aCTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGc                                             >  2:2781996/1‑93 (MQ=255)
                                            aCTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGc                                             <  1:569291/93‑1 (MQ=255)
                                              ttCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAg                                           >  2:3558311/1‑93 (MQ=255)
                                              ttCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAg                                           <  1:2093793/93‑1 (MQ=255)
                                               tCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGt                                          <  2:4077561/93‑1 (MQ=255)
                                               tCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGt                                          <  2:856000/93‑1 (MQ=255)
                                                ccGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGta                                         <  2:496231/93‑1 (MQ=255)
                                                ccGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGta                                         >  2:5458957/1‑93 (MQ=255)
                                                  gcgcCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGtata                                       <  1:1251438/93‑1 (MQ=255)
                                                   cgcCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATAc                                      <  1:5690035/93‑1 (MQ=255)
                                                      ccGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTg                                   >  2:453508/1‑93 (MQ=255)
                                                        ggggctggggctGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGc                                 <  2:6349724/93‑1 (MQ=255)
                                                        ggggctggggctGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGc                                 <  2:380856/93‑1 (MQ=255)
                                                         gggctggggctgGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCa                                >  2:4603235/1‑93 (MQ=255)
                                                         gggctggggctgGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCa                                <  1:211897/93‑1 (MQ=255)
                                                           gctggggctggCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAgg                              <  2:3755998/93‑1 (MQ=255)
                                                           gctggggctggCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAgg                              <  2:5531288/93‑1 (MQ=255)
                                                             tggggctggCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGa                            <  2:1507178/93‑1 (MQ=255)
                                                              gggcctggCTGGTCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGAc                           <  2:1081715/93‑1 (MQ=255)
                                                               gggctggCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACt                          <  1:821603/93‑1 (MQ=255)
                                                               gggctggCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACt                          >  1:2009672/1‑93 (MQ=255)
                                                                ggctggctGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTTGCAGGGACtt                         >  1:3862295/1‑93 (MQ=255)
                                                                ggctggctGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACtt                         <  1:3029533/93‑1 (MQ=255)
                                                                 gctggctgGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACttt                        <  2:3912431/93‑1 (MQ=255)
                                                                 gctggctgGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACttt                        >  2:3245674/1‑93 (MQ=255)
                                                                 gctggctgGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACttt                        <  1:3530097/93‑1 (MQ=255)
                                                                   tggctggGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTcc                      >  1:2232098/1‑93 (MQ=255)
                                                                   tggctggGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTcc                      <  2:6075467/93‑1 (MQ=255)
                                                                    ggctggGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTccc                     <  2:3349817/93‑1 (MQ=255)
                                                                    ggctggGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTccc                     <  2:2124531/93‑1 (MQ=255)
                                                                      ctggGCTCACTGGAACCCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTg                   <  1:5558016/93‑1 (MQ=255)
                                                                      ctggGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTg                   >  1:1760067/1‑93 (MQ=255)
                                                                       tggGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTgg                  >  2:1946855/1‑93 (MQ=255)
                                                                       tggGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTgg                  >  2:4939140/1‑93 (MQ=255)
                                                                        gggCTCACTGGAAACCGCCCTGCCCTTCAGTATCACTGCCAGCCTGATGCTGGAGCCACAACTGCAGTATCCCTGGCAGGGACTTTCCCTGGa                 <  1:4612751/93‑1 (MQ=255)
                                                                         ggCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGAt                <  1:1211600/93‑1 (MQ=255)
                                                                            tCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGAc             >  1:5790699/1‑93 (MQ=255)
                                                                            tCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGAc             >  2:1898742/1‑93 (MQ=255)
                                                                             cACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACg            >  1:4216587/1‑93 (MQ=255)
                                                                              aCTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACgg           >  2:4673993/1‑93 (MQ=255)
                                                                               cTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGt          <  1:5373780/93‑1 (MQ=255)
                                                                               cTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGt          >  2:6407398/1‑93 (MQ=255)
                                                                               cTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGt          <  2:3510661/93‑1 (MQ=255)
                                                                                tGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGAATTTCCCTTGACGACGGTa         >  2:1196928/1‑93 (MQ=255)
                                                                                 ggAAACCGGCCTGCCCTTAAGTATCACTGACACCCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTaa        <  1:3448129/93‑1 (MQ=255)
                                                                                  gAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAg       <  2:1051349/93‑1 (MQ=255)
                                                                                       cGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGTATGACGGTAAGGACaa  <  2:2953741/93‑1 (MQ=255)
                                                                                       cGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACaa  <  2:4977043/93‑1 (MQ=255)
                                                                                       cGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACaa  <  1:346354/93‑1 (MQ=255)
                                                                                       cGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACaa  <  2:3470580/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAA  >  NC_000913/2072092‑2072271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGCC  >  NC_000913/2072089‑2072275
                                                                                             |                                                                                             
gcaACAGGGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGC                                                                                               <  SRR4302537.6483828/91‑1 (MQ=60)
GGCACAGGGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGC                                                                                               <  SRR4302537.1715989/94‑1 (MQ=60)
GCCACAGGCACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGC                                                                                               <  SRR4302537.5825993/94‑1 (MQ=60)
 gcacgggaagCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCC                                                                                              <  SRR4302537.4751/84‑1 (MQ=60)
  CACAGGGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCT                                                                                             <  SRR4302537.926005/94‑1 (MQ=60)
    CAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGC                                                                                           >  SRR4302537.1455983/1‑94 (MQ=60)
     AGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCC                                                                                          >  SRR4302537.3370001/1‑94 (MQ=60)
     AGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCC                                                                                          >  SRR4302537.6288963/1‑94 (MQ=60)
      gtgacCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCC                                                                                         <  SRR4302537.679340/89‑1 (MQ=60)
      GGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCCGCCTGCCC                                                                                         >  SRR4302537.4891361/1‑94 (MQ=60)
       gcacCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCT                                                                                        <  SRR4302537.2553392/90‑1 (MQ=60)
       GGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCT                                                                                        <  SRR4302537.3500099/94‑1 (MQ=60)
       GGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCT                                                                                        <  SRR4302537.2489034/94‑1 (MQ=60)
       GGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCT                                                                                        <  SRR4302537.37176/94‑1 (MQ=60)
       GGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCT                                                                                        >  SRR4302537.610959/1‑94 (MQ=60)
        GAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTT                                                                                       >  SRR4302537.3876589/1‑94 (MQ=60)
          ACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCA                                                                                     >  SRR4302537.4995263/1‑94 (MQ=60)
           CCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAG                                                                                    >  SRR4302537.2450533/1‑94 (MQ=60)
            CCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGT                                                                                   <  SRR4302537.5259817/94‑1 (MQ=60)
             CGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTA                                                                                  <  SRR4302537.4415757/94‑1 (MQ=60)
             CGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTA                                                                                  >  SRR4302537.4180060/1‑94 (MQ=60)
              GCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTAT                                                                                 <  SRR4302537.4996532/94‑1 (MQ=60)
              GCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTAT                                                                                 <  SRR4302537.5769329/94‑1 (MQ=60)
              GCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCAGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTAT                                                                                 <  SRR4302537.4597590/94‑1 (MQ=60)
                CACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCA                                                                               >  SRR4302537.758992/1‑94 (MQ=60)
                  CAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACT                                                                             <  SRR4302537.1923075/94‑1 (MQ=60)
                  CAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACT                                                                             <  SRR4302537.1573915/94‑1 (MQ=60)
                   AGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTG                                                                            >  SRR4302537.2479328/1‑94 (MQ=60)
                    GCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGA                                                                           <  SRR4302537.5646259/94‑1 (MQ=60)
                    GCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGA                                                                           <  SRR4302537.303604/94‑1 (MQ=60)
                     CATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGAC                                                                          <  SRR4302537.5199342/94‑1 (MQ=60)
                      ATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACA                                                                         >  SRR4302537.2312016/1‑94 (MQ=60)
                      ATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACA                                                                         >  SRR4302537.6042455/1‑94 (MQ=60)
                       TGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGGAACCGGCCTGCCCTTCAGTATCACTGACAA                                                                        >  SRR4302537.2733684/1‑94 (MQ=60)
                       TGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAA                                                                        <  SRR4302537.233737/94‑1 (MQ=60)
                        GAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAAC                                                                       >  SRR4302537.5806251/1‑94 (MQ=60)
                        GAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAAC                                                                       >  SRR4302537.34883/1‑94 (MQ=60)
                         AAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACC                                                                      >  SRR4302537.2391062/1‑94 (MQ=60)
                         AAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACC                                                                      >  SRR4302537.3138352/1‑94 (MQ=60)
                          AAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCT                                                                     >  SRR4302537.5669383/1‑94 (MQ=60)
                          AAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCT                                                                     <  SRR4302537.3581627/94‑1 (MQ=60)
                          AAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCT                                                                     >  SRR4302537.6062714/1‑94 (MQ=60)
                           AGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTG                                                                    <  SRR4302537.4529909/94‑1 (MQ=60)
                           AGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTG                                                                    <  SRR4302537.661683/94‑1 (MQ=60)
                            GCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGA                                                                   >  SRR4302537.5646116/1‑94 (MQ=60)
                             CGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGAT                                                                  <  SRR4302537.4746532/94‑1 (MQ=60)
                              GTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATG                                                                 >  SRR4302537.5474094/1‑94 (MQ=60)
                              GTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATG                                                                 >  SRR4302537.1050283/1‑94 (MQ=60)
                              GTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATG                                                                 <  SRR4302537.2177508/94‑1 (MQ=60)
                                CATCGGACAATAACTACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCT                                                               <  SRR4302537.2825619/94‑1 (MQ=60)
                                  TCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGG                                                             <  SRR4302537.2974961/94‑1 (MQ=60)
                                   CGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGA                                                            >  SRR4302537.4461003/1‑94 (MQ=60)
                                   CGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGA                                                            <  SRR4302537.2394231/94‑1 (MQ=60)
                                    TGACAATAACGACTTCCGCGCGCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACGGACAACCTGATGCTGGAG                                                           <  SRR4302537.5777121/94‑1 (MQ=60)
                                    GGACAATAAGGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAG                                                           <  SRR4302537.5730212/94‑1 (MQ=60)
                                    GGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAG                                                           <  SRR4302537.3156025/94‑1 (MQ=60)
                                    GGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAG                                                           <  SRR4302537.3275597/94‑1 (MQ=60)
                                    GGACAATAACGACTTCCGCGCCAGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAG                                                           <  SRR4302537.4159032/94‑1 (MQ=60)
                                     GACAATAACTACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGC                                                          <  SRR4302537.1188633/94‑1 (MQ=60)
                                      ACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCC                                                         >  SRR4302537.2609959/1‑94 (MQ=60)
                                        AATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCAC                                                       >  SRR4302537.5021770/1‑94 (MQ=60)
                                          TAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAA                                                     >  SRR4302537.6151334/1‑94 (MQ=60)
                                           AACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAAC                                                    >  SRR4302537.5090717/1‑94 (MQ=60)
                                           AACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAAC                                                    >  SRR4302537.6499574/1‑94 (MQ=60)
                                               ACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCA                                                <  SRR4302537.573202/94‑1 (MQ=60)
                                                CTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAG                                               >  SRR4302537.2117708/1‑94 (MQ=60)
                                                CTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAG                                               >  SRR4302537.1796429/1‑94 (MQ=60)
                                                 TTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGT                                              <  SRR4302537.2108561/94‑1 (MQ=60)
                                                   CCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTAT                                            <  SRR4302537.5484893/94‑1 (MQ=60)
                                                    CGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATA                                           <  SRR4302537.542517/94‑1 (MQ=60)
                                                     GCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATAC                                          <  SRR4302537.1260149/94‑1 (MQ=60)
                                                      CGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACC                                         <  SRR4302537.2858231/94‑1 (MQ=60)
                                                      CGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACC                                         >  SRR4302537.6134182/1‑94 (MQ=60)
                                                      CGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACC                                         <  SRR4302537.5728952/94‑1 (MQ=60)
                                                        CCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTG                                       <  SRR4302537.1004748/94‑1 (MQ=60)
                                                         CCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGG                                      <  SRR4302537.5868948/94‑1 (MQ=60)
                                                          CGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGC                                     <  SRR4302537.1554002/94‑1 (MQ=60)
                                                          CGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGC                                     <  SRR4302537.1377714/94‑1 (MQ=60)
                                                           GGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCA                                    <  SRR4302537.2273312/94‑1 (MQ=60)
                                                            GGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAG                                   <  SRR4302537.3272124/94‑1 (MQ=60)
                                                            GGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAG                                   <  SRR4302537.213411/94‑1 (MQ=60)
                                                              GCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGG                                 >  SRR4302537.2032217/1‑94 (MQ=60)
                                                              GCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGG                                 <  SRR4302537.5773585/94‑1 (MQ=60)
                                                                 GGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACT                              >  SRR4302537.2023677/1‑94 (MQ=60)
                                                                  GGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTTGCAGGGACTT                             >  SRR4302537.3889331/1‑94 (MQ=60)
                                                                  GGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTT                             >  SRR4302537.3417948/1‑94 (MQ=60)
                                                                  GGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTT                             <  SRR4302537.827243/94‑1 (MQ=60)
                                                                   GGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTT                            <  SRR4302537.3292712/94‑1 (MQ=60)
                                                                   GGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTT                            <  SRR4302537.3050655/94‑1 (MQ=60)
                                                                    GCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTC                           <  SRR4302537.3555055/94‑1 (MQ=60)
                                                                     CTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCC                          >  SRR4302537.2247844/1‑94 (MQ=60)
                                                                       GGCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCT                        <  SRR4302537.4344271/94‑1 (MQ=60)
                                                                        GCTGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTG                       >  SRR4302537.1772338/1‑94 (MQ=60)
                                                                         CTGGGCTCACTGGAACCCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGG                      <  SRR4302537.5595990/94‑1 (MQ=60)
                                                                          TGGGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGA                     <  SRR4302537.4826607/94‑1 (MQ=60)
                                                                           GGGCTCACTGGAAACCGCCCTGCCCTTCAGTATCACTGCCAGCCTGATGCTGGAGCCACAACTGCAGTATCCCTGGCAGGGACTTTCCCTGGAT                    <  SRR4302537.4644689/94‑1 (MQ=60)
                                                                            GGCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATG                   <  SRR4302537.1220030/94‑1 (MQ=60)
                                                                             GCTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGA                  >  SRR4302537.5186730/1‑94 (MQ=60)
                                                                              CTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGAC                 >  SRR4302537.5830403/1‑94 (MQ=60)
                                                                              CTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGAC                 <  SRR4302537.1995858/94‑1 (MQ=60)
                                                                              CTCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGAC                 >  SRR4302537.5758398/1‑94 (MQ=60)
                                                                               TCACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACG                >  SRR4302537.4245980/1‑94 (MQ=60)
                                                                                CACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGG               >  SRR4302537.1253155/1‑94 (MQ=60)
                                                                                 ACTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGT              <  SRR4302537.812770/94‑1 (MQ=60)
                                                                                  CTGGAAACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTA             <  SRR4302537.5410530/94‑1 (MQ=60)
                                                                                    GGAAACCGGCCTGCCCTTAAGTATCACTGACACCCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAG           <  SRR4302537.3472520/94‑1 (MQ=60)
                                                                                       AACCGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGAC        >  SRR4302537.3979991/1‑94 (MQ=60)
                                                                                          CGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAAC     <  SRR4302537.348761/94‑1 (MQ=60)
                                                                                          CGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAAC     >  SRR4302537.1399519/1‑94 (MQ=60)
                                                                                          CGGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAAC     <  SRR4302537.4681750/94‑1 (MQ=60)
                                                                                           GGGCTTCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATCCCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACG    <  SRR4302537.2024405/94‑1 (MQ=60)
                                                                                           GGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACG    <  SRR4302537.4229735/94‑1 (MQ=60)
                                                                                           GGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACG    <  SRR4302537.5148431/94‑1 (MQ=60)
                                                                                           GGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACG    >  SRR4302537.3196066/1‑94 (MQ=60)
                                                                                           GGCCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACG    <  SRR4302537.1878190/94‑1 (MQ=60)
                                                                                             CCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGCC  <  SRR4302537.728863/94‑1 (MQ=60)
                                                                                             CCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGCC  >  SRR4302537.302170/1‑94 (MQ=60)
                                                                                             CCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGCC  <  SRR4302537.2166569/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGCC  >  NC_000913/2072089‑2072275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑