Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 172,865 C→T A468A (GCC→GCT)  fhuB → FhuB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913172,8650CT100.0% 564.4 / NA 158A468A (GCC→GCT) fhuBFhuB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (80/78);  total (80/78)

Only 100 of 174 total aligned reads displayed.

GCTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCA  >  NC_000913/172775‑172954
                                                                                          |                                                                                          
gCTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATcgctgc                                                                                          >  2:234179/1‑93 (MQ=255)
gCTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATcgctgc                                                                                          >  2:3068798/1‑93 (MQ=255)
  tGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGctgccg                                                                                        >  2:2291390/1‑93 (MQ=255)
  tGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGctgccg                                                                                        >  2:5217210/1‑93 (MQ=255)
   gTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTgccgg                                                                                       <  1:1987887/93‑1 (MQ=255)
     ttCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTgccggcc                                                                                     <  2:4051660/93‑1 (MQ=255)
       cTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCcgc                                                                                   <  2:567863/93‑1 (MQ=255)
        tGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCcgcg                                                                                  >  1:5665211/1‑93 (MQ=255)
         ggTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCgg                                                                                 >  2:3895814/1‑93 (MQ=255)
          gTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGt                                                                                <  2:3955528/93‑1 (MQ=255)
           ggCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTg                                                                               <  2:1355388/92‑1 (MQ=255)
            gCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATTATCGCTGCCGGCCGCGGTgg                                                                              >  1:3243295/1‑93 (MQ=255)
                ggTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGAttt                                                                          >  2:1368896/1‑93 (MQ=255)
                 gTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGAtttt                                                                         >  1:260776/1‑93 (MQ=255)
                   aaTGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTcc                                                                       >  1:2536947/1‑93 (MQ=255)
                     tGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGCCGCTGTTGATCATTATGCTCGCTGCCGGCCGCGGTGGATTTTcccc                                                                     >  2:424640/1‑93 (MQ=255)
                     tGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTcccc                                                                     <  2:1285785/93‑1 (MQ=255)
                        cTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCcaca                                                                  >  1:6365409/1‑93 (MQ=255)
                        cTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCcaca                                                                  >  2:4241901/1‑93 (MQ=255)
                         tttGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCacac                                                                 >  2:4773788/1‑93 (MQ=255)
                          ttGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACAcc                                                                <  2:1037425/93‑1 (MQ=255)
                             gctggctgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGGTCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTa                                                             >  1:5020287/1‑93 (MQ=255)
                             gctggctgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTa                                                             <  2:5643818/93‑1 (MQ=255)
                              gtggctgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTAt                                                            <  1:2641964/92‑1 (MQ=255)
                              ctggctgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTAt                                                            <  1:3387268/93‑1 (MQ=255)
                                ggctgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGt                                                          <  2:1448295/93‑1 (MQ=255)
                                ggctgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGt                                                          <  1:1031143/93‑1 (MQ=255)
                                 gctgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGtt                                                         <  2:4432156/93‑1 (MQ=255)
                                  ctgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTa                                                        >  1:6087110/1‑93 (MQ=255)
                                   tgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTAc                                                       <  2:10859/93‑1 (MQ=255)
                                   tgTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTAc                                                       >  1:3759968/1‑93 (MQ=255)
                                     ttACCTGCCGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTg                                                     >  2:6285112/1‑93 (MQ=255)
                                     ttACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGACCGCGGTGGATTTTCCCCACACCGTATGTTACTg                                                     >  1:2387308/1‑93 (MQ=255)
                                      tACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTgg                                                    >  1:3882258/1‑93 (MQ=255)
                                      tACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTgg                                                    >  2:4320423/1‑93 (MQ=255)
                                      tACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTgg                                                    <  2:4947913/93‑1 (MQ=255)
                                       aCCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGc                                                   <  1:3061316/93‑1 (MQ=255)
                                        ccTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCg                                                  <  1:6179404/93‑1 (MQ=255)
                                        ccTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCg                                                  <  1:4880223/93‑1 (MQ=255)
                                         cTGCAGGCAGTCTCGGCGCGGCGTTGACGCTGTGGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCgg                                                 <  1:382525/93‑1 (MQ=255)
                                          tGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCggg                                                >  1:3355770/1‑93 (MQ=255)
                                          tGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCggg                                                <  2:4781163/93‑1 (MQ=255)
                                          tGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCggg                                                >  1:2232525/1‑93 (MQ=255)
                                            caggcagTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGa                                              <  1:36442/93‑1 (MQ=255)
                                            caggcagTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGa                                              <  2:6264836/93‑1 (MQ=255)
                                            caggcagTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGa                                              <  2:1339537/93‑1 (MQ=255)
                                             aggcagTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑t                                            <  1:4442947/93‑1 (MQ=255)
                                              ggcagTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCggggggg                                            >  2:5897616/1‑90 (MQ=255)
                                              ggcagTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑Tg                                           >  1:3274330/1‑93 (MQ=255)
                                              ggcagTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑Tg                                           >  2:1737930/1‑93 (MQ=255)
                                                cagTCTCGGCGCGGCGGTGACGCTGTTCATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGc                                         <  2:3224989/93‑1 (MQ=255)
                                                  gTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGt                                       >  1:803012/1‑93 (MQ=255)
                                                  gTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGt                                       <  2:161097/93‑1 (MQ=255)
                                                    ctcGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTa                                     <  2:5592882/93‑1 (MQ=255)
                                                     tcGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑GGGCGGTaa                                    >  1:363482/1‑93 (MQ=255)
                                                      cggcgcggcgGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAg                                   <  1:2699444/93‑1 (MQ=255)
                                                        gcgcggcgGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGGGTTTAGCa                                 >  2:750134/1‑93 (MQ=255)
                                                          gcggcgGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGGTAAACAcc                               >  1:2442209/1‑93 (MQ=255)
                                                             gcggTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGGGGTAAGCACcgcg                            >  1:1659660/1‑93 (MQ=255)
                                                              cggTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGGGGATTTTCCCCACACCGTATGCTACTGGCGGGGA‑TGGCGTTAATGACCGCGt                           >  1:5830787/1‑93 (MQ=255)
                                                               ggTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATTGGGGTTAA‑CACcgcggt                          >  2:5861949/1‑91 (MQ=255)
                                                               ggTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGGGGTAAGCACCGCGtt                          >  1:4560011/1‑93 (MQ=255)
                                                               ggTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGtt                          <  2:5321942/93‑1 (MQ=255)
                                                                gTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTc                         >  2:6265457/1‑93 (MQ=255)
                                                                gTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTc                         >  2:2522762/1‑93 (MQ=255)
                                                                gTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑GGGCGTTAAGCACCGCGTTc                         >  2:6133130/1‑93 (MQ=255)
                                                                 tGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGGTAAGCACCGCGTTCa                        >  2:2983475/1‑93 (MQ=255)
                                                                  gACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCAc                       <  1:2724566/93‑1 (MQ=255)
                                                                    cGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCa                     <  2:1907912/93‑1 (MQ=255)
                                                                    cGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCa                     <  1:2745596/93‑1 (MQ=255)
                                                                    cGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCa                     <  2:353067/93‑1 (MQ=255)
                                                                     gCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGGGTTAAGCACCGCGTTccccct                    >  1:1023861/1‑91 (MQ=255)
                                                                     gCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGTTAAGCACCCCGGTTAccct                    >  2:28265/1‑91 (MQ=255)
                                                                      cTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGTTAAGCACCGCGTTCACCATg                   >  1:1918866/1‑93 (MQ=255)
                                                                       tGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCAttc                  >  1:1261624/1‑91 (MQ=255)
                                                                        gTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCt                 >  1:711009/1‑93 (MQ=255)
                                                                        gTTGATCATGATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCt                 <  2:4508454/93‑1 (MQ=255)
                                                                          tGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGTTAAGCACCGGGTTTACCATGCttt               >  1:3831207/1‑93 (MQ=255)
                                                                          tGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCttt               <  1:1340340/93‑1 (MQ=255)
                                                                          tGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGGTAAGCACCGCGTTCACCATGCttt               >  1:4534180/1‑93 (MQ=255)
                                                                           gATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGGACCGCGTTCACCATGCtttt              >  2:1260180/1‑93 (MQ=255)
                                                                           gATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCtttt              <  1:4054430/93‑1 (MQ=255)
                                                                           gATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGGTCACCCTGCtttt              >  2:2588881/1‑93 (MQ=255)
                                                                            aTCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGTTAAGCACCGCGGTCACCATGCttttt             >  1:1365543/1‑92 (MQ=255)
                                                                             tCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGT‑TGGCGTTTAACACCGCGTTCTCCATGCTTTtgt            >  2:2729347/1‑92 (MQ=255)
                                                                             tCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTtga            <  2:3161881/93‑1 (MQ=255)
                                                                             tCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTtga            <  1:2032787/93‑1 (MQ=255)
                                                                             tCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTtga            >  2:5534300/1‑93 (MQ=255)
                                                                              cATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGGTAAGCACCGCGTTCACCATGCTTTtgat           >  2:1523252/1‑93 (MQ=255)
                                                                              cATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTtgat           <  2:3167302/93‑1 (MQ=255)
                                                                                ttATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGTTTAGCAACGCGGTCACCATTCTTTtgatgg         >  2:5984209/1‑92 (MQ=255)
                                                                                ttATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTtgatga         <  1:4919166/93‑1 (MQ=255)
                                                                                ttATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGAACCGCGGTCACCATGCTTTtgatga         >  1:741170/1‑93 (MQ=255)
                                                                                 tATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGG‑TGGCGTTTAGAACCGAGCGCCCCATGCGTTTtttgat        >  2:4611946/1‑93 (MQ=255)
                                                                                 tATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTTgatgat        <  1:5596532/93‑1 (MQ=255)
                                                                                   tGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGt      <  1:6024470/93‑1 (MQ=255)
                                                                                   tGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGt      <  2:4476085/93‑1 (MQ=255)
                                                                                   tGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGATTTTGTTGATGt      >  2:2448739/1‑93 (MQ=255)
                                                                                    gATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGtt     <  1:5871316/93‑1 (MQ=255)
                                                                                       cgctgcCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGGTCACCATGCTTTTGATGATGTTGcg  >  1:4785745/1‑92 (MQ=255)
                                                                                          |                                                                                          
GCTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGA‑TGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCA  >  NC_000913/172775‑172954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGC  >  NC_000913/172774‑172957
                                                                                           |                                                                                            
TGCTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGC                                                                                            <  SRR4302537.772196/94‑1 (MQ=60)
 GCTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCC                                                                                           >  SRR4302537.2645545/1‑94 (MQ=60)
  CTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCG                                                                                          >  SRR4302537.4712110/1‑94 (MQ=60)
  CTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCG                                                                                          <  SRR4302537.1137003/94‑1 (MQ=60)
    GTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGC                                                                                        <  SRR4302537.2001708/94‑1 (MQ=60)
    GTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGC                                                                                        >  SRR4302537.3884793/1‑94 (MQ=60)
     TTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCC                                                                                       <  SRR4302537.722404/94‑1 (MQ=60)
      TTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCG                                                                                      <  SRR4302537.3265266/94‑1 (MQ=60)
        CTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCG                                                                                    >  SRR4302537.5703971/1‑94 (MQ=60)
         TGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGG                                                                                   <  SRR4302537.6044426/94‑1 (MQ=60)
            TGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATTATCGCTGCCGGCCGCGGTGG                                                                                >  SRR4302537.3266155/1‑94 (MQ=60)
             GCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGA                                                                               <  SRR4302537.3305901/94‑1 (MQ=60)
                 GGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTT                                                                           >  SRR4302537.262614/1‑94 (MQ=60)
                   TAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCC                                                                         >  SRR4302537.2554748/1‑94 (MQ=60)
                     ATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCC                                                                       >  SRR4302537.4356731/1‑94 (MQ=60)
                       GCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCAC                                                                     <  SRR4302537.2789414/94‑1 (MQ=60)
                        CCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACA                                                                    >  SRR4302537.6408824/1‑94 (MQ=60)
                         CTTTGGCTGGCTGTTACCTGCAGGCAGGCTCGGCGCGGCGGTGACGCTGTTGATCATTCTGATTGCTCCCGGCCGCGGTGGATTTTCTCCACAC                                                                   >  SRR4302537.4668741/1‑94 (MQ=60)
                           TTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCG                                                                 >  SRR4302537.3473203/1‑94 (MQ=60)
                             GGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGGTCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTA                                                               >  SRR4302537.5054863/1‑94 (MQ=60)
                              GCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCCCACCGTAT                                                              >  SRR4302537.5162365/1‑94 (MQ=60)
                              GCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTAT                                                              >  SRR4302537.2408583/1‑94 (MQ=60)
                               GTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATG                                                             <  SRR4302537.2660450/94‑1 (MQ=60)
                               CTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATG                                                             <  SRR4302537.3411264/94‑1 (MQ=60)
                                 GGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTT                                                           <  SRR4302537.1038246/94‑1 (MQ=60)
                                  GCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTA                                                          >  SRR4302537.6128928/1‑94 (MQ=60)
                                   CTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTAC                                                         >  SRR4302537.3786365/1‑94 (MQ=60)
                                     GTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCCCACCGTATGTTACTG                                                       >  SRR4302537.6344429/1‑94 (MQ=60)
                                     GTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGACCGCGGTGGATTTTCCCCACACCGTATGTTACTG                                                       >  SRR4302537.2404125/1‑94 (MQ=60)
                                      TTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGG                                                      >  SRR4302537.3909417/1‑94 (MQ=60)
                                       TACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGC                                                     <  SRR4302537.341050/94‑1 (MQ=60)
                                        ACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCG                                                    <  SRR4302537.3082649/94‑1 (MQ=60)
                                         CCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGG                                                   <  SRR4302537.4913934/94‑1 (MQ=60)
                                         CCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGG                                                   <  SRR4302537.6221968/94‑1 (MQ=60)
                                          CTGCAGGCAGTCTCGGCGCGGCGTTGACGCTGTGGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGG                                                  <  SRR4302537.385173/94‑1 (MQ=60)
                                          CTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGG                                                  <  SRR4302537.1271147/94‑1 (MQ=60)
                                          CTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGG                                                  >  SRR4302537.3379538/1‑94 (MQ=60)
                                          CTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGG                                                  >  SRR4302537.2248272/1‑94 (MQ=60)
                                          CTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGCATGTTACTGGCGGG                                                  >  SRR4302537.3990009/1‑94 (MQ=60)
                                           TGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGG                                                 >  SRR4302537.3759143/1‑94 (MQ=60)
                                             CAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGAT                                               <  SRR4302537.36720/94‑1 (MQ=60)
                                             CAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGAT                                               <  SRR4302537.2749232/94‑1 (MQ=60)
                                              AGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATG                                              >  SRR4302537.3297438/1‑94 (MQ=60)
                                              AGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATG                                              <  SRR4302537.1652066/94‑1 (MQ=60)
                                              AGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATG                                              <  SRR4302537.4473633/94‑1 (MQ=60)
                                              AGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGAGGATTTTCCCCACATCCCATGTTACTGGTGGGGATG                                              >  SRR4302537.6252768/1‑94 (MQ=60)
                                                  AGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGTGGCGT                                          >  SRR4302537.808525/1‑94 (MQ=60)
                                                  AGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGT                                          <  SRR4302537.3801936/94‑1 (MQ=60)
                                                    TCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTA                                        <  SRR4302537.2373466/94‑1 (MQ=60)
                                                     CTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGggggcggtaa                                       >  SRR4302537.366008/1‑84 (MQ=60)
                                                      TCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAG                                      <  SRR4302537.1952943/94‑1 (MQ=60)
                                                       CGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGgtgggggtaaga                                     >  SRR4302537.4568850/1‑82 (MQ=60)
                                                       CGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGC                                     <  SRR4302537.6083090/94‑1 (MQ=60)
                                                       CGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGC                                     <  SRR4302537.2718302/94‑1 (MQ=60)
                                                          CGCGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGTGGCGGTAAACACC                                  >  SRR4302537.2459401/1‑94 (MQ=60)
                                                            CGGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGC                                >  SRR4302537.2592431/1‑94 (MQ=60)
                                                             GGCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGGGGTAAGCACCGCG                               >  SRR4302537.1671294/1‑94 (MQ=60)
                                                              GCGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGGGGATTTTCCCCACACCGTATGCTACTGGCGGGGATGGCGTTAATGACCGCGT                              >  SRR4302537.5870750/1‑94 (MQ=60)
                                                               CGGTGACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGGGGTAAGCACCGCGTT                             >  SRR4302537.4591572/1‑94 (MQ=60)
                                                                   GACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACC                         <  SRR4302537.2743596/94‑1 (MQ=60)
                                                                    ACGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCA                        >  SRR4302537.4874030/1‑94 (MQ=60)
                                                                     CGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGTGGGGTTAAGCACCGCGTTCcccct                       >  SRR4302537.1030907/1‑89 (MQ=60)
                                                                     CGCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCAT                       <  SRR4302537.2764746/94‑1 (MQ=60)
                                                                      GCTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGTGGCGTTAAGCACCGCGTTCACCATG                      >  SRR4302537.1932189/1‑94 (MQ=60)
                                                                       CTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATTC                     >  SRR4302537.1270398/1‑94 (MQ=60)
                                                                       CTGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGC                     >  SRR4302537.564249/1‑94 (MQ=60)
                                                                        TGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCT                    >  SRR4302537.715875/1‑94 (MQ=60)
                                                                        TGTTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGGTAAGCACCGCGTTCACCCTGCT                    >  SRR4302537.1740081/1‑94 (MQ=60)
                                                                          TTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGTGGCGTTAAGCACCGGGTTTACCATGCTTT                  >  SRR4302537.3858061/1‑94 (MQ=60)
                                                                          TTGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGGTAAGCACCGCGTTCACCATGCTTT                  >  SRR4302537.4565572/1‑94 (MQ=60)
                                                                           TGATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTT                 <  SRR4302537.1349674/94‑1 (MQ=60)
                                                                           TGATCATTATGAGCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTT                 <  SRR4302537.88905/94‑1 (MQ=60)
                                                                            GATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGTGGCGTTAAGCACCGCGGTCACCATGCTTTTT                >  SRR4302537.1375059/1‑94 (MQ=60)
                                                                            GATCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTG                <  SRR4302537.4082771/94‑1 (MQ=60)
                                                                              TCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGGTGGCGGTAAGCACCGCGCTCACCATGCTTTTGAT              >  SRR4302537.6448477/1‑94 (MQ=60)
                                                                              TCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGAT              >  SRR4302537.4988017/1‑94 (MQ=60)
                                                                              TCATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGAT              <  SRR4302537.2046956/94‑1 (MQ=60)
                                                                                ATTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGAACCGCGGTCACCATGCTTTTGATGA            >  SRR4302537.746232/1‑94 (MQ=60)
                                                                                 TTATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGAT           <  SRR4302537.4953100/94‑1 (MQ=60)
                                                                                  TATGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATG          <  SRR4302537.5634798/94‑1 (MQ=60)
                                                                                    TGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTT        <  SRR4302537.6065867/94‑1 (MQ=60)
                                                                                    TGATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGAGGGGGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTT        >  SRR4302537.2065552/1‑94 (MQ=60)
                                                                                     GATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTG       <  SRR4302537.587722/94‑1 (MQ=60)
                                                                                     GATCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTG       <  SRR4302537.5911589/94‑1 (MQ=60)
                                                                                       TCGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGGTCACCATGCTTTTGATGATGTTGCG     >  SRR4302537.4818778/1‑94 (MQ=60)
                                                                                        CGCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAG    <  SRR4302537.2684850/94‑1 (MQ=60)
                                                                                         GCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTTATGATGTTGCAGG   >  SRR4302537.2017601/1‑94 (MQ=60)
                                                                                         GCTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGG   <  SRR4302537.5711596/94‑1 (MQ=60)
                                                                                          CTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCGCCGCATTCACCATGCTTTTGATGATGTTGCAGGC  <  SRR4302537.4705274/94‑1 (MQ=60)
                                                                                          CTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGC  >  SRR4302537.223289/1‑94 (MQ=60)
                                                                                          CTGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGC  >  SRR4302537.5849750/1‑94 (MQ=60)
                                                                                           |                                                                                            
TGCTGTTTCTGGTGCCGGGTAATGCCTTTGGCTGGCTGTTACCTGCAGGCAGTCTCGGCGCGGCGGTGACGCTGTTGATCATTATGATCGCCGCCGGCCGCGGTGGATTTTCCCCACACCGTATGTTACTGGCGGGGATGGCGTTAAGCACCGCGTTCACCATGCTTTTGATGATGTTGCAGGC  >  NC_000913/172774‑172957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑