Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 418,134 T→C V341A (GTA→GCA)  phoR → positive and negative sensor protein for pho regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913418,1340TC100.0% 750.4 / NA 217V341A (GTA→GCA) phoRpositive and negative sensor protein for pho regulon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (112/105);  total (112/105)

Only 100 of 222 total aligned reads displayed.

TGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCG  >  NC_000913/418044‑418223
                                                                                          |                                                                                         
tgtCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCAc                                                                                         >  2:2722041/1‑93 (MQ=255)
 gtCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACg                                                                                        >  1:6437684/1‑93 (MQ=255)
    tGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTg                                                                                     >  1:1204745/1‑93 (MQ=255)
    tGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTg                                                                                     <  1:971721/93‑1 (MQ=255)
       cAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCa                                                                                  <  2:556507/93‑1 (MQ=255)
        aaCGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAg                                                                                 <  1:4501226/93‑1 (MQ=255)
        aaCGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAg                                                                                 <  2:680572/93‑1 (MQ=255)
        aaCGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAg                                                                                 <  1:3646951/93‑1 (MQ=255)
        aaCGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAg                                                                                 >  2:4357776/1‑93 (MQ=255)
         aCGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGc                                                                                >  2:5164391/1‑93 (MQ=255)
           gAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGa                                                                              <  2:1595572/93‑1 (MQ=255)
           gAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGa                                                                              <  2:5448574/93‑1 (MQ=255)
           gAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGa                                                                              >  2:1516688/1‑93 (MQ=255)
           gAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGa                                                                              >  1:3235700/1‑93 (MQ=255)
            aaGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAg                                                                             <  1:5944994/93‑1 (MQ=255)
             aGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGt                                                                            >  2:3884536/1‑93 (MQ=255)
              gATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTg                                                                           >  1:5130427/1‑93 (MQ=255)
               aTCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGc                                                                          >  1:3920899/1‑93 (MQ=255)
               aTCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGc                                                                          >  2:125296/1‑93 (MQ=255)
                   gCTACGCAGCGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGACGGCACGCATATCACCGCACGCTGGCAGCGAGCGCCGCa                                                                      >  2:2887171/1‑93 (MQ=255)
                       cgaaGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGt                                                                  <  2:5747536/90‑1 (MQ=255)
                       cGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGt                                                                  <  2:4317869/93‑1 (MQ=255)
                       cGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGt                                                                  <  1:4325533/93‑1 (MQ=255)
                         cAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGTGAGTGCCGCACGGTGc                                                                <  1:180679/93‑1 (MQ=255)
                         cAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGc                                                                >  2:5286540/1‑93 (MQ=255)
                         cAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTACCGCACGGTGc                                                                <  1:3119307/93‑1 (MQ=255)
                          aGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGcc                                                               <  2:3209614/93‑1 (MQ=255)
                           gTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCg                                                              <  2:6050631/93‑1 (MQ=255)
                            tGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGa                                                             <  1:414722/93‑1 (MQ=255)
                             gCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGaa                                                            >  1:6266646/1‑93 (MQ=255)
                             gCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGaa                                                            >  1:1091525/1‑93 (MQ=255)
                              cGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAAt                                                           <  1:5633512/93‑1 (MQ=255)
                              cGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAAt                                                           >  1:524935/1‑93 (MQ=255)
                              cGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAAt                                                           <  1:5067484/93‑1 (MQ=255)
                              cGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAAt                                                           >  1:865520/1‑93 (MQ=255)
                                aTTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAAttt                                                         >  1:3851540/1‑93 (MQ=255)
                                aTTTAGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAAttt                                                         >  2:293995/1‑93 (MQ=255)
                                 tttCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTa                                                        <  2:359564/93‑1 (MQ=255)
                                 tttCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTa                                                        >  2:5859139/1‑93 (MQ=255)
                                     gAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGt                                                    >  2:5127077/1‑93 (MQ=255)
                                      aaCCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGtt                                                   <  1:535673/93‑1 (MQ=255)
                                      aaCCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGtt                                                   >  1:3554477/1‑93 (MQ=255)
                                       aCCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTg                                                  <  1:4905656/93‑1 (MQ=255)
                                       aCCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTg                                                  <  1:4577899/93‑1 (MQ=255)
                                          tGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAg                                               <  2:3980410/93‑1 (MQ=255)
                                          tGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAg                                               >  1:1481490/1‑93 (MQ=255)
                                           ggTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGa                                              <  2:4457338/93‑1 (MQ=255)
                                           ggTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGa                                              >  1:4882124/1‑93 (MQ=255)
                                           ggTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGa                                              <  1:2152278/93‑1 (MQ=255)
                                           ggTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGa                                              >  2:3064327/1‑93 (MQ=255)
                                               tataACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAc                                          <  1:5704506/93‑1 (MQ=255)
                                                ataACGCCGTGAATCATACGCCGGAAGGCACGCATATCACGGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACg                                         >  1:1125889/1‑93 (MQ=255)
                                                ataACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACg                                         <  2:1299954/93‑1 (MQ=255)
                                                ataACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACg                                         >  1:1969657/1‑93 (MQ=255)
                                                 taACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACgg                                        <  2:2368381/93‑1 (MQ=255)
                                                  aaCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGa                                       <  1:2813649/93‑1 (MQ=255)
                                                   aCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGAc                                      >  1:2575375/1‑93 (MQ=255)
                                                   aCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGAc                                      >  1:2336953/1‑93 (MQ=255)
                                                     gCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCg                                    <  2:5210849/93‑1 (MQ=255)
                                                       cGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCggg                                  >  1:517579/1‑93 (MQ=255)
                                                       cGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCggg                                  >  2:1850404/1‑93 (MQ=255)
                                                       cGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCggg                                  >  2:260801/1‑93 (MQ=255)
                                                        gTGAATCATACGCCGGAAGGCAGGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGAGTTGAAGATAATGGACAGGGc                                 >  2:4145564/1‑93 (MQ=255)
                                                         tGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCa                                >  2:6461871/1‑93 (MQ=255)
                                                         tGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCa                                <  1:4002411/93‑1 (MQ=255)
                                                         tGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCa                                <  2:4897742/93‑1 (MQ=255)
                                                          gAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCAt                               <  1:6127716/93‑1 (MQ=255)
                                                           aaTCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCAtt                              <  2:4965212/93‑1 (MQ=255)
                                                           aaTCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCAtt                              <  1:3358166/93‑1 (MQ=255)
                                                            aTCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTg                             <  2:5970845/93‑1 (MQ=255)
                                                              cATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCa                           <  2:2148041/93‑1 (MQ=255)
                                                              cATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCa                           >  2:3307587/1‑93 (MQ=255)
                                                              cATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCa                           >  1:1420947/1‑93 (MQ=255)
                                                               aTACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCAc                          >  2:5402390/1‑93 (MQ=255)
                                                                 aCGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCg                        <  1:2470142/93‑1 (MQ=255)
                                                                 aCGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCg                        >  2:3735958/1‑93 (MQ=255)
                                                                     cGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGc                    <  1:460730/93‑1 (MQ=255)
                                                                      ggAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCa                   >  1:718710/1‑93 (MQ=255)
                                                                       gAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCat                  >  1:3190158/1‑93 (MQ=255)
                                                                        aaGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCata                 <  1:3430304/93‑1 (MQ=255)
                                                                          ggCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATAtt               >  1:371438/1‑93 (MQ=255)
                                                                          ggCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATAtt               <  1:2704268/93‑1 (MQ=255)
                                                                           gCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTc              >  2:3919047/1‑93 (MQ=255)
                                                                           gCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTc              <  2:3465575/93‑1 (MQ=255)
                                                                             aCGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCcg            >  2:4840257/1‑93 (MQ=255)
                                                                             aCGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCcg            <  2:3693934/93‑1 (MQ=255)
                                                                             aCGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCcg            >  2:6361005/1‑93 (MQ=255)
                                                                               gCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCcgcg          <  2:726212/93‑1 (MQ=255)
                                                                               gCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCcgcg          <  2:1384229/93‑1 (MQ=255)
                                                                                cATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCgcgc         >  1:3932129/1‑93 (MQ=255)
                                                                                 atatCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGcc        >  1:4810389/1‑93 (MQ=255)
                                                                                    tCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGa     >  2:3811967/1‑93 (MQ=255)
                                                                                    tCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGa     >  1:438802/1‑93 (MQ=255)
                                                                                    tCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGa     >  1:6187432/1‑93 (MQ=255)
                                                                                     cACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGAc    <  2:3019843/93‑1 (MQ=255)
                                                                                     cACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGAc    >  1:6262308/1‑93 (MQ=255)
                                                                                      aCCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGAcc   <  1:2920464/93‑1 (MQ=255)
                                                                                      aCCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGAcc   >  1:5096959/1‑93 (MQ=255)
                                                                                       ccGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCg  >  1:259954/1‑93 (MQ=255)
                                                                                       ccGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCg  <  1:4059205/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCG  >  NC_000913/418044‑418223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCG  >  NC_000913/418041‑418227
                                                                                             |                                                                                             
AGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGC                                                                                               >  SRR4302537.6292952/1‑94 (MQ=60)
 GGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCA                                                                                              >  SRR4302537.397310/1‑94 (MQ=60)
   TGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACG                                                                                            >  SRR4302537.6481392/1‑94 (MQ=60)
   TGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACG                                                                                            >  SRR4302537.4817928/1‑94 (MQ=60)
      CTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTG                                                                                         >  SRR4302537.1213128/1‑94 (MQ=60)
       TGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGG                                                                                        <  SRR4302537.978418/94‑1 (MQ=60)
       TGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGG                                                                                        >  SRR4302537.1177748/1‑94 (MQ=60)
        GGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGC                                                                                       <  SRR4302537.3838209/94‑1 (MQ=60)
          CAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATACCGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAG                                                                                     <  SRR4302537.4136714/94‑1 (MQ=60)
           AACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGC                                                                                    <  SRR4302537.4532355/94‑1 (MQ=60)
           AACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGC                                                                                    <  SRR4302537.3672621/94‑1 (MQ=60)
            ACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCG                                                                                   <  SRR4302537.1644651/94‑1 (MQ=60)
             CGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGA                                                                                  >  SRR4302537.3258508/1‑94 (MQ=60)
               AAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGT                                                                                <  SRR4302537.6100259/94‑1 (MQ=60)
               AAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGT                                                                                <  SRR4302537.5985799/94‑1 (MQ=60)
                AGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTG                                                                               >  SRR4302537.5165784/1‑94 (MQ=60)
                 GATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGC                                                                              >  SRR4302537.3948304/1‑94 (MQ=60)
                    CAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGC                                                                           <  SRR4302537.2803245/94‑1 (MQ=60)
                      GCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCAC                                                                         >  SRR4302537.3495116/1‑94 (MQ=60)
                      GCTACGCAGTGCGATTGCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCAC                                                                         <  SRR4302537.124971/94‑1 (MQ=60)
                       CTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACG                                                                        <  SRR4302537.1774614/94‑1 (MQ=60)
                       CTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACG                                                                        >  SRR4302537.3821049/1‑94 (MQ=60)
                        TACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGG                                                                       >  SRR4302537.5282810/1‑94 (MQ=60)
                          CGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTG                                                                     <  SRR4302537.4355605/94‑1 (MQ=60)
                            CAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGTGAGTGCCGCACGGTGCC                                                                   <  SRR4302537.181964/94‑1 (MQ=60)
                            CAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTACCGCACGGTGCC                                                                   <  SRR4302537.3141108/94‑1 (MQ=60)
                             AGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCG                                                                  <  SRR4302537.461263/94‑1 (MQ=60)
                               TGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAA                                                                >  SRR4302537.2422279/1‑94 (MQ=60)
                               TGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAA                                                                >  SRR4302537.6309655/1‑94 (MQ=60)
                               TGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAA                                                                >  SRR4302537.6309654/1‑94 (MQ=60)
                               TGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAA                                                                >  SRR4302537.1099063/1‑94 (MQ=60)
                               TGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAA                                                                <  SRR4302537.417595/94‑1 (MQ=60)
                                GCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAAT                                                               >  SRR4302537.871457/1‑94 (MQ=60)
                                GCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAAT                                                               >  SRR4302537.528539/1‑94 (MQ=60)
                                 CGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATT                                                              >  SRR4302537.4098092/1‑94 (MQ=60)
                                 CGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATT                                                              <  SRR4302537.5672056/94‑1 (MQ=60)
                                 CGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATT                                                              <  SRR4302537.5102367/94‑1 (MQ=60)
                                  GATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTT                                                             >  SRR4302537.3878507/1‑94 (MQ=60)
                                  GATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTT                                                             >  SRR4302537.3976658/1‑94 (MQ=60)
                                  GATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTT                                                             >  SRR4302537.82606/1‑94 (MQ=60)
                                  GATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTT                                                             >  SRR4302537.2709995/1‑94 (MQ=60)
                                   ATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTA                                                            >  SRR4302537.526328/1‑94 (MQ=60)
                                    TTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAG                                                           <  SRR4302537.5121271/94‑1 (MQ=60)
                                      TCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCG                                                         <  SRR4302537.2914019/94‑1 (MQ=60)
                                       CGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGT                                                        >  SRR4302537.6302732/1‑94 (MQ=60)
                                        GAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTT                                                       >  SRR4302537.3579570/1‑94 (MQ=60)
                                         AACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTG                                                      <  SRR4302537.539348/94‑1 (MQ=60)
                                         AACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTG                                                      >  SRR4302537.5730202/1‑94 (MQ=60)
                                          ACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA                                                     <  SRR4302537.4939519/94‑1 (MQ=60)
                                          ACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA                                                     <  SRR4302537.4609578/94‑1 (MQ=60)
                                          ACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGA                                                     >  SRR4302537.2601479/1‑94 (MQ=60)
                                            CTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAG                                                   >  SRR4302537.1491794/1‑94 (MQ=60)
                                            CTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAG                                                   <  SRR4302537.5361602/94‑1 (MQ=60)
                                             TGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGA                                                  >  SRR4302537.4915848/1‑94 (MQ=60)
                                              GGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGAT                                                 <  SRR4302537.2167449/94‑1 (MQ=60)
                                              GGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGAT                                                 <  SRR4302537.4517841/94‑1 (MQ=60)
                                               GTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATA                                                <  SRR4302537.948381/94‑1 (MQ=60)
                                                 CTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC                                              <  SRR4302537.2881894/94‑1 (MQ=60)
                                                 CTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC                                              <  SRR4302537.6218312/94‑1 (MQ=60)
                                                 CTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAAC                                              <  SRR4302537.2583132/94‑1 (MQ=60)
                                                  TATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACGGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACG                                             >  SRR4302537.1133695/1‑94 (MQ=60)
                                                  TATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACG                                             >  SRR4302537.1983356/1‑94 (MQ=60)
                                                  TATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACG                                             <  SRR4302537.5743548/94‑1 (MQ=60)
                                                  TATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACG                                             <  SRR4302537.6127600/94‑1 (MQ=60)
                                                   ATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGG                                            <  SRR4302537.6453846/94‑1 (MQ=60)
                                                    TAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGA                                           <  SRR4302537.4877021/94‑1 (MQ=60)
                                                     AACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGAC                                          >  SRR4302537.3597232/1‑94 (MQ=60)
                                                     AACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGAC                                          >  SRR4302537.2353432/1‑94 (MQ=60)
                                                     AACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGAC                                          >  SRR4302537.2593405/1‑94 (MQ=60)
                                                     AACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGAC                                          <  SRR4302537.2833323/94‑1 (MQ=60)
                                                       CGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCTAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCG                                        <  SRR4302537.4580932/94‑1 (MQ=60)
                                                         CCGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGG                                      >  SRR4302537.521143/1‑94 (MQ=60)
                                                          CGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGC                                     >  SRR4302537.3696830/1‑94 (MQ=60)
                                                          CGTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGC                                     >  SRR4302537.1948539/1‑94 (MQ=60)
                                                           GTGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCA                                    >  SRR4302537.2122305/1‑94 (MQ=60)
                                                            TGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCAT                                   <  SRR4302537.3955963/94‑1 (MQ=60)
                                                            TGAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCAT                                   <  SRR4302537.4030431/94‑1 (MQ=60)
                                                             GAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATT                                  <  SRR4302537.3252915/94‑1 (MQ=60)
                                                             GAATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATT                                  <  SRR4302537.6169831/94‑1 (MQ=60)
                                                              AATCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTG                                 <  SRR4302537.3381954/94‑1 (MQ=60)
                                                                TCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCA                               <  SRR4302537.5449399/94‑1 (MQ=60)
                                                                TCATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCA                               >  SRR4302537.1430842/1‑94 (MQ=60)
                                                                 CATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCAC                              >  SRR4302537.3082681/1‑94 (MQ=60)
                                                                 CATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCAC                              <  SRR4302537.5201382/94‑1 (MQ=60)
                                                                  ATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACC                             <  SRR4302537.4602729/94‑1 (MQ=60)
                                                                  ATACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACC                             >  SRR4302537.3168533/1‑94 (MQ=60)
                                                                   TACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCG                            >  SRR4302537.419943/1‑94 (MQ=60)
                                                                   TACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCG                            <  SRR4302537.4921721/94‑1 (MQ=60)
                                                                    ACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGG                           <  SRR4302537.2487508/94‑1 (MQ=60)
                                                                    ACGCCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGG                           >  SRR4302537.3418075/1‑94 (MQ=60)
                                                                       CCGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGC                        >  SRR4302537.254847/1‑94 (MQ=60)
                                                                        CGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCA                       >  SRR4302537.4395356/1‑94 (MQ=60)
                                                                        CGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCA                       >  SRR4302537.723621/1‑94 (MQ=60)
                                                                        CGGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCA                       <  SRR4302537.463902/94‑1 (MQ=60)
                                                                         GGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCAT                      >  SRR4302537.5037807/1‑94 (MQ=60)
                                                                         GGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCAT                      >  SRR4302537.3212593/1‑94 (MQ=60)
                                                                         GGAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCAT                      >  SRR4302537.5862443/1‑94 (MQ=60)
                                                                          GAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATA                     <  SRR4302537.641781/94‑1 (MQ=60)
                                                                          GAAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATA                     <  SRR4302537.3917848/94‑1 (MQ=60)
                                                                           AAGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATAT                    <  SRR4302537.3454580/94‑1 (MQ=60)
                                                                            AGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATT                   <  SRR4302537.2253653/94‑1 (MQ=60)
                                                                            AGGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATT                   >  SRR4302537.374013/1‑94 (MQ=60)
                                                                             GGCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTC                  <  SRR4302537.2723148/94‑1 (MQ=60)
                                                                              GCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCC                 <  SRR4302537.4057993/94‑1 (MQ=60)
                                                                              GCACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCC                 <  SRR4302537.1106056/94‑1 (MQ=60)
                                                                               CACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCG                <  SRR4302537.5060852/94‑1 (MQ=60)
                                                                               CACGCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCG                >  SRR4302537.6504662/1‑94 (MQ=60)
                                                                                  GCATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGC             >  SRR4302537.3959604/1‑94 (MQ=60)
                                                                                   CATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCC            >  SRR4302537.4843593/1‑94 (MQ=60)
                                                                                    ATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCT           <  SRR4302537.3284389/94‑1 (MQ=60)
                                                                                    ATATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCT           >  SRR4302537.513696/1‑94 (MQ=60)
                                                                                      ATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGGGCATATTCCGCGCCTGA         >  SRR4302537.3383843/1‑94 (MQ=60)
                                                                                      ATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGA         >  SRR4302537.441855/1‑94 (MQ=60)
                                                                                      ATCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGA         >  SRR4302537.6230056/1‑94 (MQ=60)
                                                                                       TCACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGAC        >  SRR4302537.6305298/1‑94 (MQ=60)
                                                                                        CACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACC       <  SRR4302537.1488900/94‑1 (MQ=60)
                                                                                        CACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACC       >  SRR4302537.5132059/1‑94 (MQ=60)
                                                                                         ACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCG      <  SRR4302537.2940989/94‑1 (MQ=60)
                                                                                         ACCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCG      >  SRR4302537.261790/1‑94 (MQ=60)
                                                                                          CCGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGA     <  SRR4302537.4087574/94‑1 (MQ=60)
                                                                                           CGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAG    <  SRR4302537.4448535/94‑1 (MQ=60)
                                                                                           CGCACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAG    >  SRR4302537.4761857/1‑94 (MQ=60)
                                                                                             CACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCG  <  SRR4302537.432673/94‑1 (MQ=60)
                                                                                             CACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCG  >  SRR4302537.5425163/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGGTGTCTGGCAACGAAGATCAGCTACGCAGTGCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACCGTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGCATTGCACCGGAGCATATTCCGCGCCTGACCGAGCG  >  NC_000913/418041‑418227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑