Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	704,236	T→C	L357S (TTG→TCG)	nagE →	PTS system, N‑acetylglucosamine‑specific enzyme IIABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	704,236	0	T	C	99.6%	856.0 / ‑5.4	246	L357S (TTG→TCG)	nagE	PTS system, N‑acetylglucosamine‑specific enzyme IIABC
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (131/114); minor base A (1/0); total (132/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 250 total aligned reads displayed.

GTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAG  >  NC_000913/704147‑704325

 tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                       <  2:2038079/93‑1 (MQ=255)

 tataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGtt                                                                                       >  1:1513300/1‑93 (MQ=255)

 tataACCTGCCGGCCGCCAGCCAGAACGTCTGCATGCTACTGGTGATGGGCGTTATCTTCTTCGCTATGTACTTCGTGGTGTTCAGTTCGGtt                                                                                       <  2:3040733/93‑1 (MQ=255)

  ataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTa                                                                                      >  1:6369955/1‑93 (MQ=255)

  ataACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTa                                                                                      >  2:4041876/1‑93 (MQ=255)

   taACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTAt                                                                                     >  1:3775985/1‑93 (MQ=255)

    aaCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATc                                                                                    <  1:484905/93‑1 (MQ=255)

    aaCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATc                                                                                    <  1:1395220/93‑1 (MQ=255)

    aaCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATc                                                                                    <  2:900200/93‑1 (MQ=255)

     aCCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATcc                                                                                   >  2:5754377/1‑93 (MQ=255)

        tGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCa                                                                                >  1:997758/1‑93 (MQ=255)

        tGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCa                                                                                <  1:3424095/93‑1 (MQ=255)

        tGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCa                                                                                >  2:4840516/1‑93 (MQ=255)

         gccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAt                                                                               <  1:2102471/93‑1 (MQ=255)

          ccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATg                                                                              >  1:5255432/1‑93 (MQ=255)

          ccggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATg                                                                              >  1:5076913/1‑93 (MQ=255)

            ggccgCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGtt                                                                            <  1:5368842/93‑1 (MQ=255)

             gccgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTc                                                                           <  1:6474302/93‑1 (MQ=255)

              ccgcgAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCa                                                                          <  2:3842435/93‑1 (MQ=255)

               cgccAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCaa                                                                         >  1:6235471/1‑93 (MQ=255)

                 ccagccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAcc                                                                       <  1:2866016/93‑1 (MQ=255)

                 ccagccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAAcc                                                                       >  1:188739/1‑93 (MQ=255)

                    gccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGa                                                                    <  2:4328241/93‑1 (MQ=255)

                    gccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGa                                                                    >  1:1027826/1‑93 (MQ=255)

                     ccagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                   >  2:1239256/1‑93 (MQ=255)

                     ccagAACGTCTGGATGCTGATGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaa                                                                   >  2:6315056/1‑93 (MQ=255)

                      cagAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaa                                                                  >  1:1727951/1‑93 (MQ=255)

                       cgAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTGCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 <  2:4860005/92‑1 (MQ=255)

                       agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 <  2:6457021/93‑1 (MQ=255)

                       agAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGaaaa                                                                 >  2:5010278/1‑93 (MQ=255)

                        caaCGTCTGGATGCTGCTCGGTATGGGTTTTATCTTATTCGCTACCTACTTCGTGGTTTTCAGTGCGGTTATCCGCATGTACAACCTGAAAAc                                                                <  2:5898114/92‑1 (MQ=255)

                           cGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGcc                                                             >  1:1758942/1‑93 (MQ=255)

                            gTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCg                                                            <  2:2658299/93‑1 (MQ=255)

                            gTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCg                                                            <  1:1135570/93‑1 (MQ=255)

                               tGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCgtgt                                                         <  2:1839490/93‑1 (MQ=255)

                               tGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGt                                                         >  1:530525/1‑93 (MQ=255)

                                 gATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCg                                                       <  2:960292/93‑1 (MQ=255)

                                 gATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCg                                                       >  1:2550842/1‑93 (MQ=255)

                                  aTGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGt                                                      <  1:4472828/93‑1 (MQ=255)

                                  aTGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGt                                                      >  2:4683588/1‑93 (MQ=255)

                                   tgctgcTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTg                                                     >  2:3264731/1‑93 (MQ=255)

                                    gctgctGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGa                                                    <  1:4396314/93‑1 (MQ=255)

                                     ctgctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGaa                                                   <  1:4214204/93‑1 (MQ=255)

                                       gctgGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCTACCTGAAAACGCCGGGTCGTGAAGa                                                 >  2:2211103/1‑93 (MQ=255)

                                          ggTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATaa                                              >  2:4094699/1‑93 (MQ=255)

                                            tGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaag                                            >  1:3076606/1‑93 (MQ=255)

                                             gATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaaga                                           <  1:3040834/93‑1 (MQ=255)

                                              aTGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaa                                          >  2:5388822/1‑93 (MQ=255)

                                               tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         >  1:5972004/1‑93 (MQ=255)

                                               tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         >  2:5528518/1‑93 (MQ=255)

                                               tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         <  2:3550454/93‑1 (MQ=255)

                                               tGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAaagaag                                         <  2:5999930/93‑1 (MQ=255)

                                                gggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAagaaga                                        <  1:4025886/93‑1 (MQ=255)

                                                gggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAagaaga                                        >  2:3483141/1‑93 (MQ=255)

                                                gggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAagaaga                                        <  2:2294559/93‑1 (MQ=255)

                                                gggCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAagaaga                                        <  2:2089891/93‑1 (MQ=255)

                                                  gCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACg                                      <  1:3529652/93‑1 (MQ=255)

                                                   cGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACga                                     <  2:5619885/93‑1 (MQ=255)

                                                   cGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACga                                     <  1:1856504/93‑1 (MQ=255)

                                                    gTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgag                                    <  1:4988989/93‑1 (MQ=255)

                                                     ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   <  2:5090827/93‑1 (MQ=255)

                                                     ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   <  1:949193/93‑1 (MQ=255)

                                                     ttATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACgaga                                   <  2:6372242/93‑1 (MQ=255)

                                                      tATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAt                                  >  2:3713193/1‑93 (MQ=255)

                                                      tATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAt                                  <  1:2268406/93‑1 (MQ=255)

                                                       aTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATc                                 <  2:1079039/93‑1 (MQ=255)

                                                         cttcttCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGt                               <  1:1301449/93‑1 (MQ=255)

                                                          ttcttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGtt                              >  1:5024642/1‑93 (MQ=255)

                                                            cttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTAc                            <  2:5236658/93‑1 (MQ=255)

                                                            cttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGAATGTTCAACCAGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTAc                            <  1:4550586/93‑1 (MQ=255)

                                                             ttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACt                           >  1:6276249/1‑93 (MQ=255)

                                                             ttcGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACt                           <  1:2816450/93‑1 (MQ=255)

                                                                gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                        <  2:3608350/93‑1 (MQ=255)

                                                                gCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaa                        >  1:248833/1‑93 (MQ=255)

                                                                 cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaag                       >  1:1228628/1‑93 (MQ=255)

                                                                 cTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaag                       >  2:5773742/1‑93 (MQ=255)

                                                                  tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaata                      >  1:4723125/1‑91 (MQ=255)

                                                                  tATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTgaaga                      >  1:1445390/1‑93 (MQ=255)

                                                                    tCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGaagaag                    >  2:6051010/1‑93 (MQ=255)

                                                                    tCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGaagaag                    >  2:6114059/1‑93 (MQ=255)

                                                                    tCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGaagaag                    <  1:3618504/93‑1 (MQ=255)

                                                                       tcTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCa                 <  1:5456329/92‑1 (MQ=255)

                                                                        cTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTGAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCaa                <  2:1433549/93‑1 (MQ=255)

                                                                        cTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCaa                <  2:249240/93‑1 (MQ=255)

                                                                         ttCGTGGTTTTCAGTTCGGATATCCGCATGTTCAACCTGAAAGCGCCGGGTAGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc               <  2:682701/93‑1 (MQ=255)

                                                                         ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAc               >  1:4322542/1‑93 (MQ=255)

                                                                         ttCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGACAAc               >  1:3365774/1‑93 (MQ=255)

                                                                           cGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAg             <  1:2775918/93‑1 (MQ=255)

                                                                             tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCa           >  2:2724496/1‑93 (MQ=255)

                                                                             tggtgTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCa           >  2:1644034/1‑93 (MQ=255)

                                                                               gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAac         <  1:5754377/93‑1 (MQ=255)

                                                                               gtgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAac         <  1:2566566/93‑1 (MQ=255)

                                                                                tgtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAaca        >  1:2925936/1‑93 (MQ=255)

                                                                                 gtTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAacac       >  1:4602995/1‑93 (MQ=255)

                                                                                  ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACt      <  2:1985218/93‑1 (MQ=255)

                                                                                  ttCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACt      <  1:548815/93‑1 (MQ=255)

                                                                                    cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTga    >  1:2375388/1‑93 (MQ=255)

                                                                                    cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTga    >  2:4071077/1‑93 (MQ=255)

                                                                                    cAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTga    <  1:1083153/93‑1 (MQ=255)

                                                                                     aGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTgaa   >  2:2926447/1‑93 (MQ=255)

GTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAG  >  NC_000913/704147‑704325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  >  NC_000913/704143‑704329

TGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTC                                                                                               >  SRR4302537.6447848/1‑94 (MQ=60)

TGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTC                                                                                               >  SRR4302537.1650851/1‑94 (MQ=60)

TGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTC                                                                                               >  SRR4302537.4136126/1‑94 (MQ=60)

 GATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCG                                                                                              <  SRR4302537.2585338/94‑1 (MQ=60)

  ATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGG                                                                                             <  SRR4302537.3166735/94‑1 (MQ=60)

    GTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTT                                                                                           >  SRR4302537.1523873/1‑94 (MQ=60)

    GTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTT                                                                                           <  SRR4302537.5582950/94‑1 (MQ=60)

     TATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTA                                                                                          >  SRR4302537.6413398/1‑94 (MQ=60)

      ATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTAT                                                                                         >  SRR4302537.3802498/1‑94 (MQ=60)

        AACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCC                                                                                       <  SRR4302537.488227/94‑1 (MQ=60)

        AACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCC                                                                                       <  SRR4302537.1404947/94‑1 (MQ=60)

         ACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCG                                                                                      >  SRR4302537.1249644/1‑94 (MQ=60)

         ACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCG                                                                                      <  SRR4302537.1074650/94‑1 (MQ=60)

          CCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGC                                                                                     >  SRR4302537.1093786/1‑94 (MQ=60)

           CTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCA                                                                                    >  SRR4302537.1003674/1‑94 (MQ=60)

           CTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCA                                                                                    >  SRR4302537.2248025/1‑94 (MQ=60)

           CTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCA                                                                                    >  SRR4302537.1004641/1‑94 (MQ=60)

            TGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCAT                                                                                   <  SRR4302537.3448334/94‑1 (MQ=60)

             GCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATG                                                                                  >  SRR4302537.5111861/1‑94 (MQ=60)

             GCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATG                                                                                  >  SRR4302537.5291595/1‑94 (MQ=60)

             GCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATG                                                                                  <  SRR4302537.2117297/94‑1 (MQ=60)

               CGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTT                                                                                >  SRR4302537.4416968/1‑94 (MQ=60)

                GGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTC                                                                               <  SRR4302537.5405576/94‑1 (MQ=60)

                 GCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCA                                                                              <  SRR4302537.6518243/94‑1 (MQ=60)

                 GCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCA                                                                              >  SRR4302537.4279944/1‑94 (MQ=60)

                 GCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCA                                                                              <  SRR4302537.6359883/94‑1 (MQ=60)

                  CCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAA                                                                             >  SRR4302537.6278354/1‑94 (MQ=60)

                  CCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAA                                                                             >  SRR4302537.6297799/1‑94 (MQ=60)

                    GCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACC                                                                           >  SRR4302537.190079/1‑94 (MQ=60)

                     CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          >  SRR4302537.5437412/1‑94 (MQ=60)

                     CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          >  SRR4302537.4723642/1‑94 (MQ=60)

                     CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          <  SRR4302537.4601547/94‑1 (MQ=60)

                     CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          >  SRR4302537.1881760/1‑94 (MQ=60)

                     CCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCT                                                                          <  SRR4302537.2886120/94‑1 (MQ=60)

                       AGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGA                                                                        >  SRR4302537.1034902/1‑94 (MQ=60)

                        GCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAA                                                                       >  SRR4302537.1296482/1‑94 (MQ=60)

                         CCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAA                                                                      >  SRR4302537.1740020/1‑94 (MQ=60)

                         CCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAA                                                                      >  SRR4302537.6083545/1‑94 (MQ=60)

                          CAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAA                                                                     <  SRR4302537.5110255/94‑1 (MQ=60)

                           AGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAC                                                                    >  SRR4302537.3569651/1‑94 (MQ=60)

                           AGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAAC                                                                    <  SRR4302537.5520863/94‑1 (MQ=60)

                              ACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCC                                                                 >  SRR4302537.1771202/1‑94 (MQ=60)

                                GTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGG                                                               <  SRR4302537.1143459/94‑1 (MQ=60)

                                GTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGG                                                               <  SRR4302537.1407708/94‑1 (MQ=60)

                                GTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTAGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGG                                                               >  SRR4302537.3049211/1‑94 (MQ=60)

                                 TCTGGATGCTGGTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGG                                                              <  SRR4302537.5266582/94‑1 (MQ=60)

                                 TCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGG                                                              >  SRR4302537.5991684/1‑94 (MQ=60)

                                 TCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGG                                                              >  SRR4302537.4973249/1‑94 (MQ=60)

                                  CTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGT                                                             >  SRR4302537.534163/1‑94 (MQ=60)

                                    GGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCG                                                           <  SRR4302537.3439752/94‑1 (MQ=60)

                                    GGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCG                                                           >  SRR4302537.6466333/1‑94 (MQ=60)

                                    GGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCG                                                           >  SRR4302537.2568707/1‑94 (MQ=60)

                                      gtgaTGCTGGTGATGGGCGTTGTCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCACCCTGAAAACGCCGGGTCGTG                                                         <  SRR4302537.286800/90‑1 (MQ=60)

                                      ATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTG                                                         <  SRR4302537.4503771/94‑1 (MQ=60)

                                        TCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAA                                                       <  SRR4302537.3652504/94‑1 (MQ=60)

                                        GCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAA                                                       <  SRR4302537.4426642/94‑1 (MQ=60)

                                         CTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAG                                                      <  SRR4302537.4243591/94‑1 (MQ=60)

                                            CTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATA                                                   >  SRR4302537.4213475/1‑94 (MQ=60)

                                            CTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATA                                                   >  SRR4302537.4531202/1‑94 (MQ=60)

                                              GGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAA                                                 >  SRR4302537.4028480/1‑94 (MQ=60)

                                              GGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAA                                                 <  SRR4302537.4417878/94‑1 (MQ=60)

                                               GTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAG                                                >  SRR4302537.5766460/1‑94 (MQ=60)

                                               GTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAG                                                >  SRR4302537.3098037/1‑94 (MQ=60)

                                                 GATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAA                                              <  SRR4302537.3062023/94‑1 (MQ=60)

                                                  ATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAG                                             >  SRR4302537.6012993/1‑94 (MQ=60)

                                                  ATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAG                                             >  SRR4302537.1720038/1‑94 (MQ=60)

                                                  ATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTAGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAG                                             >  SRR4302537.3491908/1‑94 (MQ=60)

                                                    GGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAC                                           <  SRR4302537.4054071/94‑1 (MQ=60)

                                                    GGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAC                                           >  SRR4302537.1724660/1‑94 (MQ=60)

                                                    GCGCGTTATCTTCTTCGCTATCTACTTCGTTGTGTTCAGTTCGGTTATCCGCATGTTCACCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGAC                                           <  SRR4302537.2317195/94‑1 (MQ=60)

                                                      GCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGA                                         <  SRR4302537.3554609/94‑1 (MQ=60)

                                                       GGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTCCGGTTATCCGCAGGTTCAGCCTGAAAACGCCGGGTCGTGAAGATAAAGAAGGCGAG                                        <  SRR4302537.258460/94‑1 (MQ=60)

                                                       CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        <  SRR4302537.4547381/94‑1 (MQ=60)

                                                       CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        >  SRR4302537.4810152/1‑94 (MQ=60)

                                                       CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        >  SRR4302537.2733159/1‑94 (MQ=60)

                                                       CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        >  SRR4302537.1326307/1‑94 (MQ=60)

                                                       CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        <  SRR4302537.1869435/94‑1 (MQ=60)

                                                       CGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAG                                        <  SRR4302537.1778480/94‑1 (MQ=60)

                                                        GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       <  SRR4302537.5023374/94‑1 (MQ=60)

                                                        GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302537.2496630/1‑94 (MQ=60)

                                                        GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       >  SRR4302537.2055405/1‑94 (MQ=60)

                                                        GTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGA                                       <  SRR4302537.929041/94‑1 (MQ=60)

                                                         TTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAT                                      <  SRR4302537.955758/94‑1 (MQ=60)

                                                         TTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGAT                                      <  SRR4302537.3070632/94‑1 (MQ=60)

                                                          TATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATC                                     <  SRR4302537.2938860/94‑1 (MQ=60)

                                                          TATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATC                                     <  SRR4302537.2284426/94‑1 (MQ=60)

                                                          TATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATC                                     <  SRR4302537.5386751/94‑1 (MQ=60)

                                                           ATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCG                                    >  SRR4302537.1748902/1‑94 (MQ=60)

                                                           ATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCG                                    <  SRR4302537.4470814/94‑1 (MQ=60)

                                                           ATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGCGTCGTGAAGATAAAGAAGACGAGATCT                                    >  SRR4302537.532112/1‑94 (MQ=60)

                                                            TCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGT                                   >  SRR4302537.1120131/1‑94 (MQ=60)

                                                             CTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTT                                  >  SRR4302537.5059249/1‑94 (MQ=60)

                                                             CTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTT                                  >  SRR4302537.2851487/1‑94 (MQ=60)

                                                             CTTCTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTT                                  <  SRR4302537.1310499/94‑1 (MQ=60)

                                                                CTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACT                               >  SRR4302537.5040818/1‑94 (MQ=60)

                                                                CTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACT                               >  SRR4302537.6319311/1‑94 (MQ=60)

                                                                CTTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGAATGTTCAACCAGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACT                               <  SRR4302537.4582077/94‑1 (MQ=60)

                                                                 TTCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTG                              <  SRR4302537.2836142/94‑1 (MQ=60)

                                                                  TCGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGA                             >  SRR4302537.4133236/1‑94 (MQ=60)

                                                                   CGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAA                            >  SRR4302537.250605/1‑94 (MQ=60)

                                                                   CGCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAA                            >  SRR4302537.2363350/1‑94 (MQ=60)

                                                                    GCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGACGATAAAGAAGACGAGATCGTTACTGACG                           >  SRR4302537.5411902/1‑94 (MQ=60)

                                                                    GCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAG                           >  SRR4302537.4708003/1‑94 (MQ=60)

                                                                    GCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAG                           >  SRR4302537.3049995/1‑94 (MQ=60)

                                                                    GCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAG                           >  SRR4302537.1237173/1‑94 (MQ=60)

                                                                    GCTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAG                           >  SRR4302537.1150078/1‑94 (MQ=60)

                                                                     CTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAATA                          >  SRR4302537.4755775/1‑94 (MQ=60)

                                                                     CTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGA                          >  SRR4302537.193299/1‑94 (MQ=60)

                                                                     CTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGA                          >  SRR4302537.1455462/1‑94 (MQ=60)

                                                                     CTATCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGA                          <  SRR4302537.3701181/94‑1 (MQ=60)

                                                                        TCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGC                       >  SRR4302537.1435262/1‑94 (MQ=60)

                                                                        TCTACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGC                       <  SRR4302537.3644011/94‑1 (MQ=60)

                                                                           TCTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAA                    <  SRR4302537.5493546/94‑1 (MQ=60)

                                                                           ACTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAA                    >  SRR4302537.2554645/1‑94 (MQ=60)

                                                                            CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAC                   >  SRR4302537.4352593/1‑94 (MQ=60)

                                                                            CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAAC                   <  SRR4302537.3139972/94‑1 (MQ=60)

                                                                            CTTCGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGACAAC                   >  SRR4302537.3389614/1‑94 (MQ=60)

                                                                               CGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGC                <  SRR4302537.2795323/94‑1 (MQ=60)

                                                                               CGTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGC                >  SRR4302537.1462846/1‑94 (MQ=60)

                                                                                GTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCA               >  SRR4302537.5334102/1‑94 (MQ=60)

                                                                                GTGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCA               <  SRR4302537.6102802/94‑1 (MQ=60)

                                                                                 TGGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGACAACAGCAA              >  SRR4302537.6333090/1‑94 (MQ=60)

                                                                                  GGTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAAC             <  SRR4302537.3541484/94‑1 (MQ=60)

                                                                                   GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACA            >  SRR4302537.2797073/1‑94 (MQ=60)

                                                                                   GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACA            <  SRR4302537.5793792/94‑1 (MQ=60)

                                                                                   GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACA            >  SRR4302537.5088866/1‑94 (MQ=60)

                                                                                   GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACA            <  SRR4302537.2584548/94‑1 (MQ=60)

                                                                                   GTGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACA            >  SRR4302537.2946484/1‑94 (MQ=60)

                                                                                    TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACAC           >  SRR4302537.4634856/1‑94 (MQ=60)

                                                                                    TGTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACAC           <  SRR4302537.2635285/94‑1 (MQ=60)

                                                                                     GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACT          >  SRR4302537.4934581/1‑94 (MQ=60)

                                                                                     GTTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACT          <  SRR4302537.1773328/94‑1 (MQ=60)

                                                                                      TTCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTG         <  SRR4302537.552595/94‑1 (MQ=60)

                                                                                       TCAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGA        >  SRR4302537.2392126/1‑94 (MQ=60)

                                                                                        CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAA       >  SRR4302537.2520383/1‑94 (MQ=60)

                                                                                        CAGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAA       <  SRR4302537.1090628/94‑1 (MQ=60)

                                                                                         AGTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAG      >  SRR4302537.655181/1‑94 (MQ=60)

                                                                                          GTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGA     >  SRR4302537.3288560/1‑94 (MQ=60)

                                                                                          GTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGA     <  SRR4302537.3339581/94‑1 (MQ=60)

                                                                                          GTTCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGA     >  SRR4302537.1329260/1‑94 (MQ=60)

                                                                                            TCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAG   <  SRR4302537.5587141/94‑1 (MQ=60)

                                                                                            TCGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAG   >  SRR4302537.4950455/1‑94 (MQ=60)

                                                                                             CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  <  SRR4302537.2082153/94‑1 (MQ=60)

                                                                                             CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  <  SRR4302537.3334743/94‑1 (MQ=60)

                                                                                             CGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  <  SRR4302537.121255/94‑1 (MQ=60)

TGATGTATAACCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGCGTTATCTTCTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAGACGAGATCGTTACTGAAGAAGCCAACAGCAACACTGAAGAAGG  >  NC_000913/704143‑704329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`