Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,985,245	A→G	T100A (ACG→GCG)	yecI →	ferritin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,985,245	0	A	G	100.0%	951.9 / NA	271	T100A (ACG→GCG)	yecI	ferritin‑like protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (151/120); total (151/120)

Only 100 of 272 total aligned reads displayed.

GTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCAT  >  NC_000913/1985155‑1985334

gTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCg                                                                                         >  1:307223/1‑93 (MQ=255)

 ttAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGt                                                                                        >  1:4208488/1‑93 (MQ=255)

 ttAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGt                                                                                        >  1:413479/1‑93 (MQ=255)

 ttAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGt                                                                                        <  2:3851115/93‑1 (MQ=255)

  tAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGtt                                                                                       <  2:4459836/93‑1 (MQ=255)

   aaaGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTg                                                                                      <  1:5627349/93‑1 (MQ=255)

   aaaGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTg                                                                                      >  1:87544/1‑93 (MQ=255)

   aaaGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTg                                                                                      <  2:1826185/93‑1 (MQ=255)

     aGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGc                                                                                    >  1:5408790/1‑93 (MQ=255)

     aGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGc                                                                                    <  1:5780726/93‑1 (MQ=255)

      gCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGca                                                                                   >  1:1113448/1‑93 (MQ=255)

       ccATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGcac                                                                                  <  1:991989/93‑1 (MQ=255)

       ccATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGcac                                                                                  <  1:798414/93‑1 (MQ=255)

        cATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGcaca                                                                                 <  1:4891123/93‑1 (MQ=255)

        cATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGcaca                                                                                 <  2:612160/93‑1 (MQ=255)

         aTTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAg                                                                                <  2:4996389/93‑1 (MQ=255)

         aTTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAg                                                                                >  1:4438102/1‑93 (MQ=255)

          ttGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGt                                                                               <  1:4573197/93‑1 (MQ=255)

          ttGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGt                                                                               <  1:4027725/93‑1 (MQ=255)

           tGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGtt                                                                              >  2:5351311/1‑93 (MQ=255)

            gATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTa                                                                             >  1:2680367/1‑93 (MQ=255)

            gATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTa                                                                             <  1:2528461/93‑1 (MQ=255)

             aTGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAg                                                                            <  1:5940808/93‑1 (MQ=255)

                 tCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGa                                                                        <  1:1569169/93‑1 (MQ=255)

                    cGGTGAGAAACTGACCTCTCTGAAAGAACTGTTCCAAAAAACAATGGAAGAATACGAGCAACGTGCTAGTGCGTTGGCACAGTTAGCCGATGa                                                                     <  2:1759876/93‑1 (MQ=255)

                    cGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGa                                                                     <  2:4248645/93‑1 (MQ=255)

                    cGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGa                                                                     <  2:6402919/93‑1 (MQ=255)

                    cGGTGAAAAACCGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGa                                                                     <  1:5137470/93‑1 (MQ=255)

                      gTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAg                                                                   >  2:1364938/1‑93 (MQ=255)

                      gTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAg                                                                   >  2:5868689/1‑93 (MQ=255)

                       tGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGc                                                                  <  1:357751/93‑1 (MQ=255)

                        gAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCg                                                                 <  1:3369027/93‑1 (MQ=255)

                         aaaaaCTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATAAGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGa                                                                >  1:3701489/1‑93 (MQ=255)

                          aaaaCTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGaa                                                               <  1:3683097/93‑1 (MQ=255)

                             aCTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGa                                                            >  2:2947387/1‑93 (MQ=255)

                               tGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAAc                                                          <  2:1623796/93‑1 (MQ=255)

                                 aaCTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTg                                                        <  2:4029069/93‑1 (MQ=255)

                                 aaCTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTg                                                        >  2:5127558/1‑93 (MQ=255)

                                  aCTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGa                                                       >  2:5505116/1‑93 (MQ=255)

                                  aCTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGGTAGCCGATGAAGGGAAAGAACTGa                                                       >  2:4104340/1‑93 (MQ=255)

                                   ctctctGGAAGAACTGTTCCAAAAAGCGATGGAAGAATGCGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGaa                                                      <  1:6467859/93‑1 (MQ=255)

                                   ctctctGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGaa                                                      >  2:5537077/1‑93 (MQ=255)

                                   ctctctGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTATAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGaa                                                      <  1:3468577/93‑1 (MQ=255)

                                    tctctGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAat                                                     >  2:5790084/1‑93 (MQ=255)

                                       ctGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAatgat                                                  >  1:2340771/1‑93 (MQ=255)

                                         ggAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAAtgatga                                                >  2:4227546/1‑93 (MQ=255)

                                         ggAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAAtgatga                                                <  1:3137371/93‑1 (MQ=255)

                                           aagaaCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGAtt                                              >  1:2017701/1‑93 (MQ=255)

                                           aagaaCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGAtt                                              >  2:5288157/1‑93 (MQ=255)

                                            agaaCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTc                                             <  1:5229106/93‑1 (MQ=255)

                                            agaaCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTc                                             <  1:1836594/93‑1 (MQ=255)

                                              atcTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCaa                                           <  2:2301430/91‑1 (MQ=255)

                                              aaCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCaa                                           >  2:2274182/1‑93 (MQ=255)

                                              aaCTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCaa                                           >  1:508148/1‑93 (MQ=255)

                                                cTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAAcc                                         >  1:6166229/1‑93 (MQ=255)

                                                  gTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGt                                       >  2:3209700/1‑93 (MQ=255)

                                                   ttCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTc                                      >  1:729965/1‑93 (MQ=255)

                                                     ccAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCaa                                    <  2:1700065/93‑1 (MQ=255)

                                                     ccAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCaa                                    <  1:6076222/93‑1 (MQ=255)

                                                      cAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAt                                   <  1:5017918/93‑1 (MQ=255)

                                                      cAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAt                                   <  2:3139317/93‑1 (MQ=255)

                                                       aaaaaaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAtt                                  >  2:1559889/1‑93 (MQ=255)

                                                       aaaaaaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAtt                                  >  1:1519276/1‑93 (MQ=255)

                                                       aaaaaaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAtt                                  <  2:4875018/93‑1 (MQ=255)

                                                       aaaaaaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAtt                                  <  2:5769109/93‑1 (MQ=255)

                                                       aaaaaaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAtt                                  <  1:6340314/93‑1 (MQ=255)

                                                        aaaaaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAttt                                 >  1:2835026/1‑93 (MQ=255)

                                                        aaaaaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAttt                                 >  2:2515646/1‑93 (MQ=255)

                                                        aaaaaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAAATATGATTCAACCCTCACttt                                 >  2:6141261/1‑93 (MQ=255)

                                                           aaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCt                              <  2:5395817/93‑1 (MQ=255)

                                                           aaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCt                              >  1:4794558/1‑93 (MQ=255)

                                                           aaCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCt                              >  2:3298880/1‑93 (MQ=255)

                                                             cGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTgc                            >  1:2910903/1‑93 (MQ=255)

                                                              gATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTgcg                           >  1:3870384/1‑93 (MQ=255)

                                                              gATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTgcg                           >  2:2705282/1‑93 (MQ=255)

                                                              gATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTgcg                           >  1:30049/1‑93 (MQ=255)

                                                               aTGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTgcgc                          >  2:4708192/1‑93 (MQ=255)

                                                                tGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGcgcg                         >  2:571436/1‑93 (MQ=255)

                                                                tGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGcgcg                         >  2:1229806/1‑93 (MQ=255)

                                                                 ggAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGa                        >  2:2159163/1‑93 (MQ=255)

                                                                  gaagaaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGAt                       >  2:6279104/1‑93 (MQ=255)

                                                                  gaagaaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGAt                       >  2:5977661/1‑93 (MQ=255)

                                                                   aagaaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATc                      <  1:2943086/93‑1 (MQ=255)

                                                                    agaaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCACCCGTCAATTTCCTGCGCGATCt                     <  1:2303408/93‑1 (MQ=255)

                                                                    agaaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCt                     <  1:2692652/93‑1 (MQ=255)

                                                                    agaaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCt                     >  1:5469098/1‑93 (MQ=255)

                                                                    agaaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCt                     <  2:3764207/93‑1 (MQ=255)

                                                                      aaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTgg                   <  2:1320206/93‑1 (MQ=255)

                                                                      aaTACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTgg                   >  1:5345842/1‑93 (MQ=255)

                                                                          cGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGaaaa               >  2:3499050/1‑93 (MQ=255)

                                                                           gAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGaaaaa              >  2:2638011/1‑93 (MQ=255)

                                                                             gCAACGTTCTAGTGTGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGa            >  2:558270/1‑93 (MQ=255)

                                                                               aaCGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAAc          <  1:1030327/93‑1 (MQ=255)

                                                                               aaCGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAAc          <  1:1389729/93‑1 (MQ=255)

                                                                                   ttCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAAcagca      >  2:4933534/1‑93 (MQ=255)

                                                                                    tCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAAcagcag     <  2:1946681/93‑1 (MQ=255)

                                                                                      tAGTGCGTTGGCACATTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAgcagca   <  2:5471213/93‑1 (MQ=255)

                                                                                      tAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAgcagca   <  1:4306089/93‑1 (MQ=255)

                                                                                       aGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCat  <  2:150894/93‑1 (MQ=255)

                                                                                       aGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCat  >  2:4732404/1‑93 (MQ=255)

GTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCAT  >  NC_000913/1985155‑1985334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATG  >  NC_000913/1985154‑1985338

CGTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCG                                                                                             >  SRR4302537.309353/1‑94 (MQ=60)

 GTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGT                                                                                            >  SRR4302537.5469598/1‑94 (MQ=60)

 GTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGT                                                                                            >  SRR4302537.4237849/1‑94 (MQ=60)

 GTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGT                                                                                            >  SRR4302537.416342/1‑94 (MQ=60)

 GTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAAAGATGGAAGAATACGAGCAACGTTCTAGTGCGT                                                                                            >  SRR4302537.5038013/1‑94 (MQ=60)

   TAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTG                                                                                          >  SRR4302537.88178/1‑94 (MQ=60)

    AAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGG                                                                                         <  SRR4302537.5665846/94‑1 (MQ=60)

    AAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGG                                                                                         >  SRR4302537.4590983/1‑94 (MQ=60)

     AAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGC                                                                                        >  SRR4302537.5445679/1‑94 (MQ=60)

     AAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGC                                                                                        >  SRR4302537.486341/1‑94 (MQ=60)

      AGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCA                                                                                       >  SRR4302537.1121134/1‑94 (MQ=60)

      AGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCA                                                                                       <  SRR4302537.5820345/94‑1 (MQ=60)

        CCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACA                                                                                     <  SRR4302537.998841/94‑1 (MQ=60)

        CCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACA                                                                                     <  SRR4302537.803894/94‑1 (MQ=60)

        CCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACA                                                                                     >  SRR4302537.3074837/1‑94 (MQ=60)

         CATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAG                                                                                    <  SRR4302537.4924897/94‑1 (MQ=60)

         CATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAG                                                                                    <  SRR4302537.2865849/94‑1 (MQ=60)

         CATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAG                                                                                    >  SRR4302537.4468743/1‑94 (MQ=60)

          ATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGT                                                                                   >  SRR4302537.685682/1‑94 (MQ=60)

          ATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGT                                                                                   >  SRR4302537.5790107/1‑94 (MQ=60)

          ATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGT                                                                                   <  SRR4302537.5899015/94‑1 (MQ=60)

           TTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTT                                                                                  <  SRR4302537.4055921/94‑1 (MQ=60)

           TTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTT                                                                                  <  SRR4302537.4604851/94‑1 (MQ=60)

           TTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTT                                                                                  <  SRR4302537.1686795/94‑1 (MQ=60)

            TGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTA                                                                                 >  SRR4302537.2699082/1‑94 (MQ=60)

             GATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAG                                                                                <  SRR4302537.2546202/94‑1 (MQ=60)

              ATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGC                                                                               <  SRR4302537.4428663/94‑1 (MQ=60)

              ATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGC                                                                               <  SRR4302537.5981582/94‑1 (MQ=60)

                GTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCG                                                                             >  SRR4302537.5033238/1‑94 (MQ=60)

                  TCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGAT                                                                           <  SRR4302537.1580117/94‑1 (MQ=60)

                   CCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATG                                                                          >  SRR4302537.4072335/1‑94 (MQ=60)

                    CCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGA                                                                         >  SRR4302537.659113/1‑94 (MQ=60)

                    CCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGA                                                                         <  SRR4302537.5444650/94‑1 (MQ=60)

                     CGGTGAAAAACCGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAA                                                                        <  SRR4302537.5172873/94‑1 (MQ=60)

                        TGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCG                                                                     <  SRR4302537.3150455/94‑1 (MQ=60)

                        TGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCG                                                                     <  SRR4302537.360233/94‑1 (MQ=60)

                         GAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGA                                                                    >  SRR4302537.28312/1‑94 (MQ=60)

                         GAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGA                                                                    <  SRR4302537.3392892/94‑1 (MQ=60)

                         GAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGA                                                                    <  SRR4302537.4244520/94‑1 (MQ=60)

                         GAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATAAGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGA                                                                    >  SRR4302537.3727526/1‑94 (MQ=60)

                          AAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGCA                                                                   >  SRR4302537.2708595/1‑94 (MQ=60)

                           AAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAA                                                                  <  SRR4302537.3708998/94‑1 (MQ=60)

                             AACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGA                                                                >  SRR4302537.5126249/1‑94 (MQ=60)

                             AACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGA                                                                <  SRR4302537.1289274/94‑1 (MQ=60)

                                TGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACT                                                             >  SRR4302537.808120/1‑94 (MQ=60)

                                TGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACT                                                             >  SRR4302537.2416002/1‑94 (MQ=60)

                                TGAACTCTCTGGAAGAACTCTTCCAAAAAGCGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACT                                                             <  SRR4302537.1042888/94‑1 (MQ=60)

                                  AACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGA                                                           >  SRR4302537.5660700/1‑94 (MQ=60)

                                  AACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGA                                                           >  SRR4302537.3170197/1‑94 (MQ=60)

                                    CTCTCTGGAAGAACTGTTCCAAAAAGCGATGGAAGAATGCGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAAT                                                         <  SRR4302537.6511682/94‑1 (MQ=60)

                                    CTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAAT                                                         >  SRR4302537.3051056/1‑94 (MQ=60)

                                    CTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTATAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAAT                                                         <  SRR4302537.3493113/94‑1 (MQ=60)

                                    CTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACCTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAAT                                                         <  SRR4302537.4220908/94‑1 (MQ=60)

                                       TCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGAT                                                      >  SRR4302537.2357269/1‑94 (MQ=60)

                                        CTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATG                                                     >  SRR4302537.6449283/1‑94 (MQ=60)

                                        CTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATG                                                     >  SRR4302537.4788014/1‑94 (MQ=60)

                                          GGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGAT                                                   <  SRR4302537.413539/94‑1 (MQ=60)

                                          GGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGAT                                                   >  SRR4302537.3700726/1‑94 (MQ=60)

                                          GGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGAT                                                   <  SRR4302537.3159391/94‑1 (MQ=60)

                                           GAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATT                                                  >  SRR4302537.6289168/1‑94 (MQ=60)

                                           GAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATT                                                  >  SRR4302537.2031768/1‑94 (MQ=60)

                                           GAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATT                                                  <  SRR4302537.2459300/94‑1 (MQ=60)

                                            AAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTA                                                 >  SRR4302537.3438323/1‑94 (MQ=60)

                                             AGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCA                                                <  SRR4302537.5265066/94‑1 (MQ=60)

                                             AGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCA                                                <  SRR4302537.1849377/94‑1 (MQ=60)

                                             AGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCA                                                <  SRR4302537.1663162/94‑1 (MQ=60)

                                              GAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAA                                               >  SRR4302537.511636/1‑94 (MQ=60)

                                               AACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAAC                                              <  SRR4302537.4344198/94‑1 (MQ=60)

                                                ACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACC                                             >  SRR4302537.6208672/1‑94 (MQ=60)

                                                 CTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCG                                            <  SRR4302537.779704/94‑1 (MQ=60)

                                                  TGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGT                                           >  SRR4302537.3715167/1‑94 (MQ=60)

                                                   GTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTC                                          >  SRR4302537.734951/1‑94 (MQ=60)

                                                   GTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTC                                          >  SRR4302537.2808017/1‑94 (MQ=60)

                                                    TTCAAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCA                                         <  SRR4302537.1171221/94‑1 (MQ=60)

                                                     TCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAA                                        >  SRR4302537.5354466/1‑94 (MQ=60)

                                                      CCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAT                                       >  SRR4302537.351864/1‑94 (MQ=60)

                                                      CCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAT                                       <  SRR4302537.3405247/94‑1 (MQ=60)

                                                      CCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAT                                       <  SRR4302537.6117977/94‑1 (MQ=60)

                                                       CAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATT                                      <  SRR4302537.2961209/94‑1 (MQ=60)

                                                       CAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATT                                      <  SRR4302537.5052485/94‑1 (MQ=60)

                                                       CAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATT                                      >  SRR4302537.1529893/1‑94 (MQ=60)

                                                        AAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTT                                     <  SRR4302537.3892907/94‑1 (MQ=60)

                                                        AAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTT                                     >  SRR4302537.2854851/1‑94 (MQ=60)

                                                        AAAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTT                                     <  SRR4302537.6383636/94‑1 (MQ=60)

                                                         AAAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTC                                    >  SRR4302537.5512540/1‑94 (MQ=60)

                                                          AAAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCC                                   >  SRR4302537.1402727/1‑94 (MQ=60)

                                                           AAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCT                                  >  SRR4302537.4827646/1‑94 (MQ=60)

                                                           AAACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAAGTTCCT                                  >  SRR4302537.5089629/1‑94 (MQ=60)

                                                            AACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTG                                 >  SRR4302537.1566818/1‑94 (MQ=60)

                                                             ACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGC                                <  SRR4302537.632506/94‑1 (MQ=60)

                                                             ACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGC                                >  SRR4302537.2931353/1‑94 (MQ=60)

                                                             ACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGC                                <  SRR4302537.2336778/94‑1 (MQ=60)

                                                             ACGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTGAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGC                                >  SRR4302537.3493527/1‑94 (MQ=60)

                                                              CGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCG                               <  SRR4302537.1429823/94‑1 (MQ=60)

                                                              CGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCG                               <  SRR4302537.5390161/94‑1 (MQ=60)

                                                              CGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCG                               <  SRR4302537.6391542/94‑1 (MQ=60)

                                                              CGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCG                               >  SRR4302537.3897467/1‑94 (MQ=60)

                                                              CGATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCG                               >  SRR4302537.30290/1‑94 (MQ=60)

                                                               GATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGC                              >  SRR4302537.39213/1‑94 (MQ=60)

                                                               GATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGC                              >  SRR4302537.3018896/1‑94 (MQ=60)

                                                                ATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCG                             >  SRR4302537.2656674/1‑94 (MQ=60)

                                                                ATGGAAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCG                             >  SRR4302537.6189551/1‑94 (MQ=60)

                                                                    AAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCT                         <  SRR4302537.5220845/94‑1 (MQ=60)

                                                                    AAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCT                         >  SRR4302537.1866869/1‑94 (MQ=60)

                                                                    AAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCT                         >  SRR4302537.5506405/1‑94 (MQ=60)

                                                                    AAGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCT                         <  SRR4302537.2963747/94‑1 (MQ=60)

                                                                     AGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCACCCGTCAATTTCCTGCGCGATCTG                        <  SRR4302537.2319666/94‑1 (MQ=60)

                                                                     AGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTG                        <  SRR4302537.2711475/94‑1 (MQ=60)

                                                                     AGAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTG                        >  SRR4302537.3892069/1‑94 (MQ=60)

                                                                      GAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGG                       >  SRR4302537.4915519/1‑94 (MQ=60)

                                                                      GAATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGG                       >  SRR4302537.5382468/1‑94 (MQ=60)

                                                                      GAATACGAGCAACGTTCTAGTGCGTTGACACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGG                       <  SRR4302537.3117110/94‑1 (MQ=60)

                                                                       AATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGA                      >  SRR4302537.3947652/1‑94 (MQ=60)

                                                                        ATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAA                     >  SRR4302537.2907516/1‑94 (MQ=60)

                                                                        ATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAA                     >  SRR4302537.4280336/1‑94 (MQ=60)

                                                                        ATACGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAA                     >  SRR4302537.2693872/1‑94 (MQ=60)

                                                                           CGAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAA                  <  SRR4302537.5491462/94‑1 (MQ=60)

                                                                            GAGCAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAG                 <  SRR4302537.195499/94‑1 (MQ=60)

                                                                               CAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAAC              <  SRR4302537.524022/94‑1 (MQ=60)

                                                                               CAACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAAC              >  SRR4302537.2243114/1‑94 (MQ=60)

                                                                                AACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACA             <  SRR4302537.1037423/94‑1 (MQ=60)

                                                                                AACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACA             <  SRR4302537.5160256/94‑1 (MQ=60)

                                                                                AACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACA             >  SRR4302537.603820/1‑94 (MQ=60)

                                                                                AACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACA             >  SRR4302537.5926733/1‑94 (MQ=60)

                                                                                AACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACA             <  SRR4302537.1399421/94‑1 (MQ=60)

                                                                                 ACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAG            >  SRR4302537.865428/1‑94 (MQ=60)

                                                                                 ACGTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAG            >  SRR4302537.931241/1‑94 (MQ=60)

                                                                                  CGTTCTAGTGCGTTGGCACAG‑TAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCA          >  SRR4302537.6112053/1‑94 (MQ=60)

                                                                                   GTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCA          >  SRR4302537.364700/1‑94 (MQ=60)

                                                                                   GTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCA          <  SRR4302537.48083/94‑1 (MQ=60)

                                                                                   GTTCTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCA          <  SRR4302537.2950657/94‑1 (MQ=60)

                                                                                      CTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCA       >  SRR4302537.981691/1‑94 (MQ=60)

                                                                                      CTAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCA       <  SRR4302537.2223538/94‑1 (MQ=60)

                                                                                       TAGTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCAT      <  SRR4302537.4336023/94‑1 (MQ=60)

                                                                                         GTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGA    >  SRR4302537.5471093/1‑94 (MQ=60)

                                                                                         GTGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGA    <  SRR4302537.4226193/94‑1 (MQ=60)

                                                                                          TGCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGAT   <  SRR4302537.744538/94‑1 (MQ=60)

                                                                                           GCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATG  <  SRR4302537.3349782/94‑1 (MQ=60)

                                                                                           GCGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATG  >  SRR4302537.6121532/1‑94 (MQ=60)

CGTTAAAGCCATTGATGTTCCCGGTGAAAAACTGAACTCTCTGGAAGAACTGTTCCAAAAAACGATGGAAGAATACGAGCAACGTTCTAGTACGTTGGCACAGTTAGCCGATGAAGCGAAAGAACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATG  >  NC_000913/1985154‑1985338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`