Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,419,101	T→C	L154P (CTG→CCG)	yfcG →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,419,101	0	T	C	99.6%	824.2 / NA	236	L154P (CTG→CCG)	yfcG	putative S‑transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (123/112); minor base G (0/1); total (123/113)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

AAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATC  >  NC_000913/2419011‑2419190

 aaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCggg                                                                                        <  1:337580/93‑1 (MQ=255)

  aCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGa                                                                                       <  2:5819491/93‑1 (MQ=255)

  aCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGa                                                                                       <  1:5579724/93‑1 (MQ=255)

  aCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGa                                                                                       <  1:3443399/93‑1 (MQ=255)

   ccATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAg                                                                                      <  2:859738/93‑1 (MQ=255)

   ccATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAg                                                                                      <  2:6121117/93‑1 (MQ=255)

    cATTCCTTACGCTATTGAACGTTATCAGGTTGAAAATCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAgg                                                                                     >  2:1382494/1‑93 (MQ=255)

     aTTCCTTACGCTATTGAACGTTATCAGGTTTAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGc                                                                                    <  2:3731597/93‑1 (MQ=255)

     aTTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGc                                                                                    <  1:2875611/93‑1 (MQ=255)

     aTTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGc                                                                                    <  2:46804/93‑1 (MQ=255)

      ttCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCg                                                                                   >  2:1668137/1‑93 (MQ=255)

      ttCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCg                                                                                   >  2:120520/1‑93 (MQ=255)

       tCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCga                                                                                  >  1:3154658/1‑93 (MQ=255)

         cTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCgaga                                                                                <  1:1995616/93‑1 (MQ=255)

         cTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCgaga                                                                                <  2:4108554/93‑1 (MQ=255)

         cTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCgaga                                                                                <  1:684477/93‑1 (MQ=255)

          ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGaa                                                                               <  2:3068465/93‑1 (MQ=255)

          ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGaa                                                                               >  1:4561846/1‑93 (MQ=255)

          ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGaa                                                                               <  2:4493101/93‑1 (MQ=255)

           tACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAAc                                                                              <  2:3207785/93‑1 (MQ=255)

           tACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAAc                                                                              <  2:2765990/93‑1 (MQ=255)

           tACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAAc                                                                              >  2:2482880/1‑93 (MQ=255)

             cGCTATTGAACGTTATCAGGTTGAACCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTa                                                                            <  2:3522788/93‑1 (MQ=255)

             cGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTa                                                                            >  1:1844734/1‑93 (MQ=255)

               cTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACa                                                                          >  2:4762960/1‑93 (MQ=255)

                tATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAg                                                                         >  1:3855436/1‑93 (MQ=255)

                tATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAg                                                                         >  1:2946284/1‑93 (MQ=255)

                   tGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCAt                                                                      <  2:4784155/93‑1 (MQ=255)

                    gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCAtt                                                                     <  2:4714367/93‑1 (MQ=255)

                    gAACGTTATCAGGTTGAAACGCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCAtt                                                                     >  2:6223449/1‑93 (MQ=255)

                     aaCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTg                                                                    >  2:6077932/1‑93 (MQ=255)

                      aCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGc                                                                   <  1:4611643/93‑1 (MQ=255)

                      aCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGc                                                                   <  2:5349345/93‑1 (MQ=255)

                      aCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGc                                                                   <  1:5056277/93‑1 (MQ=255)

                       cGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCg                                                                  <  2:2214072/93‑1 (MQ=255)

                        gTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGGCCTGGCCCGGCGGCGAGAACTACAGAATTGCgg                                                                 >  1:6131979/1‑93 (MQ=255)

                            tCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGatat                                                             <  1:6335853/93‑1 (MQ=255)

                              aGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTg                                                           <  1:2515474/93‑1 (MQ=255)

                               ggTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGc                                                          >  1:5740985/1‑93 (MQ=255)

                               ggTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGc                                                          <  2:2010217/93‑1 (MQ=255)

                               ggTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGc                                                          >  2:108476/1‑93 (MQ=255)

                                gTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGGGGCGAGAACTACAGCATTGCGGATATTGcc                                                         <  1:4264709/93‑1 (MQ=255)

                                gTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                                                         <  1:2299013/93‑1 (MQ=255)

                                gTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                                                         >  1:3263003/1‑93 (MQ=255)

                                gTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCT‑GAAAACTCGCCCTGGCCGGGAGGGGGGGAATACAGCATTGCGGGTATTGCct                                                        >  2:3815859/1‑93 (MQ=255)

                                 ttGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                                                        <  2:2868563/93‑1 (MQ=255)

                                 ttGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                                                        >  1:1026266/1‑93 (MQ=255)

                                   gAACCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgc                                                      <  2:434295/93‑1 (MQ=255)

                                   gAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgc                                                      <  1:5422729/93‑1 (MQ=255)

                                   gAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgc                                                      <  2:1958590/93‑1 (MQ=255)

                                     aaCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                                                    >  2:66127/1‑93 (MQ=255)

                                      aCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTgg                                                   <  2:6347487/93‑1 (MQ=255)

                                      aCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTgg                                                   <  1:5841648/93‑1 (MQ=255)

                                      aCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTgg                                                   >  2:3362537/1‑93 (MQ=255)

                                       cTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGc                                                  >  1:4869129/1‑93 (MQ=255)

                                       cTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGc                                                  <  1:2323996/93‑1 (MQ=255)

                                        tCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGTGGCGAGAACTACAGCATTGCTGATATTGCCTGCTGGcc                                                 >  1:1151612/1‑93 (MQ=255)

                                        tCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGcc                                                 <  2:4319945/93‑1 (MQ=255)

                                          aGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGt                                               >  1:4023053/1‑93 (MQ=255)

                                           gCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTg                                              >  2:2979005/1‑93 (MQ=255)

                                           gCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAAATCGTCCTGGCCGGGAGGCGAGGACTATAGCATTACGGACATTGACTGCTGGCCGTg                                              >  2:4460650/1‑93 (MQ=255)

                                            cGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTgg                                             >  1:2441550/1‑93 (MQ=255)

                                            cGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTgg                                             <  1:5983827/93‑1 (MQ=255)

                                            cGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTgg                                             >  1:5276527/1‑93 (MQ=255)

                                            cGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTgg                                             <  2:2094159/93‑1 (MQ=255)

                                             gTCTTTCCCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTggg                                            <  1:2727271/93‑1 (MQ=255)

                                             gTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTggg                                            >  2:148619/1‑93 (MQ=255)

                                               cTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGtt                                          >  2:5997028/1‑93 (MQ=255)

                                                 ttACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTaa                                        <  2:2542968/93‑1 (MQ=255)

                                                 ttACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTaa                                        <  1:3561145/93‑1 (MQ=255)

                                                  tACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAt                                       <  1:626221/93‑1 (MQ=255)

                                                     cATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGcc                                    >  1:4831552/1‑93 (MQ=255)

                                                     cATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGcc                                    <  1:1554925/93‑1 (MQ=255)

                                                     cATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGcc                                    >  2:5501354/1‑93 (MQ=255)

                                                     cATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGcc                                    >  2:1268019/1‑93 (MQ=255)

                                                      aTGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCt                                   >  2:1777691/1‑93 (MQ=255)

                                                      aTGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCt                                   >  1:913674/1‑93 (MQ=255)

                                                        gTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGGGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTgg                                 >  2:5359105/1‑93 (MQ=255)

                                                           cTGAACAAGCTGCTGAAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACt                              <  2:4868581/93‑1 (MQ=255)

                                                           cTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACt                              >  2:5141550/1‑93 (MQ=255)

                                                                cAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCa                         <  1:3771107/93‑1 (MQ=255)

                                                                 aaGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAg                        >  2:3770210/1‑93 (MQ=255)

                                                                 aaGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAg                        <  2:1840876/93‑1 (MQ=255)

                                                                 aaGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCATGCTGGCCGTGGGTTAATGCCTGGACTCGCCAg                        >  1:4376874/1‑93 (MQ=255)

                                                                  aGCGGGTGGAAAACTCGCCCTGGCCGGAATGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGGCTCGCCAGc                       <  2:5528288/93‑1 (MQ=255)

                                                                  aGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGc                       <  2:3442173/93‑1 (MQ=255)

                                                                   gCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCg                      <  1:1475354/93‑1 (MQ=255)

                                                                     ggCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGaa                    <  2:4988827/93‑1 (MQ=255)

                                                                      gCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAAt                   <  2:3893896/93‑1 (MQ=255)

                                                                       cTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAAtt                  <  1:5951756/93‑1 (MQ=255)

                                                                       cTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAAtt                  >  1:4202429/1‑93 (MQ=255)

                                                                          gAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGAc               <  2:57231/93‑1 (MQ=255)

                                                                            aaaCTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCt             >  1:4494376/1‑93 (MQ=255)

                                                                               cTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGc          <  1:1722495/93‑1 (MQ=255)

                                                                                 cGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCaa        <  2:665421/93‑1 (MQ=255)

                                                                                   cccTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATg      <  1:5500370/93‑1 (MQ=255)

                                                                                   cccTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATg      >  1:1637024/1‑93 (MQ=255)

                                                                                     ctggccggGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTa    <  1:2792543/93‑1 (MQ=255)

                                                                                     ctggccggGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGAGAATTGACCTAGCAATGTa    >  1:2160176/1‑93 (MQ=255)

                                                                                      tggccggGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTAt   >  2:2928955/1‑93 (MQ=255)

AAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATC  >  NC_000913/2419011‑2419190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGG  >  NC_000913/2419008‑2419193

CCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCC                                                                                              >  SRR4302537.5975435/1‑94 (MQ=60)

CCCAAACCATTCCTCACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCC                                                                                              <  SRR4302537.4691729/94‑1 (MQ=60)

 CCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCG                                                                                             >  SRR4302537.1173692/1‑94 (MQ=60)

 CCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCG                                                                                             >  SRR4302537.1837694/1‑94 (MQ=60)

 CCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCG                                                                                             >  SRR4302537.2224552/1‑94 (MQ=60)

  CAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGG                                                                                            >  SRR4302537.601822/1‑94 (MQ=60)

    AACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGA                                                                                          >  SRR4302537.3721390/1‑94 (MQ=60)

    AACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGA                                                                                          <  SRR4302537.339918/94‑1 (MQ=60)

     ACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAG                                                                                         <  SRR4302537.3467761/94‑1 (MQ=60)

     ACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAG                                                                                         <  SRR4302537.5617859/94‑1 (MQ=60)

     ACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAG                                                                                         >  SRR4302537.5599818/1‑94 (MQ=60)

     ACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAG                                                                                         >  SRR4302537.2209524/1‑94 (MQ=60)

      CCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGG                                                                                        <  SRR4302537.61813/94‑1 (MQ=60)

      CCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGG                                                                                        >  SRR4302537.5481622/1‑94 (MQ=60)

       CATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGC                                                                                       <  SRR4302537.2789207/94‑1 (MQ=60)

        ATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCG                                                                                      <  SRR4302537.3227884/94‑1 (MQ=60)

        ATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCG                                                                                      >  SRR4302537.2357462/1‑94 (MQ=60)

        ATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCG                                                                                      >  SRR4302537.3442833/1‑94 (MQ=60)

        ATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCG                                                                                      <  SRR4302537.2895791/94‑1 (MQ=60)

        ATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCG                                                                                      >  SRR4302537.2868110/1‑94 (MQ=60)

         TTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGA                                                                                     >  SRR4302537.3176806/1‑94 (MQ=60)

          TCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAG                                                                                    >  SRR4302537.4045691/1‑94 (MQ=60)

          TCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAG                                                                                    >  SRR4302537.5147341/1‑94 (MQ=60)

           CCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGA                                                                                   >  SRR4302537.4476388/1‑94 (MQ=60)

            CTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAA                                                                                  <  SRR4302537.2009491/94‑1 (MQ=60)

            CTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAA                                                                                  >  SRR4302537.4593424/1‑94 (MQ=60)

            CTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAA                                                                                  >  SRR4302537.5197794/1‑94 (MQ=60)

            CTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAA                                                                                  >  SRR4302537.2572687/1‑94 (MQ=60)

            CTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAA                                                                                  <  SRR4302537.689171/94‑1 (MQ=60)

              TACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACT                                                                                <  SRR4302537.6032249/94‑1 (MQ=60)

               ACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTA                                                                               >  SRR4302537.1857572/1‑94 (MQ=60)

                  CTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAG                                                                            >  SRR4302537.3882423/1‑94 (MQ=60)

                  CTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAG                                                                            >  SRR4302537.3556160/1‑94 (MQ=60)

                  CTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAG                                                                            >  SRR4302537.4562768/1‑94 (MQ=60)

                  CTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAG                                                                            >  SRR4302537.2966965/1‑94 (MQ=60)

                  CTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAG                                                                            >  SRR4302537.247361/1‑94 (MQ=60)

                   TATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGC                                                                           <  SRR4302537.114813/94‑1 (MQ=60)

                   TATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGC                                                                           <  SRR4302537.4259435/94‑1 (MQ=60)

                    ATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCA                                                                          <  SRR4302537.4014533/94‑1 (MQ=60)

                    ATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCA                                                                          >  SRR4302537.1366717/1‑94 (MQ=60)

                       GAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTG                                                                       <  SRR4302537.4991344/94‑1 (MQ=60)

                       GAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTG                                                                       >  SRR4302537.5517950/1‑94 (MQ=60)

                        AACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGC                                                                      >  SRR4302537.2888774/1‑94 (MQ=60)

                         ACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCG                                                                     <  SRR4302537.4643575/94‑1 (MQ=60)

                         ACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCG                                                                     <  SRR4302537.4102333/94‑1 (MQ=60)

                         ACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCG                                                                     <  SRR4302537.5091084/94‑1 (MQ=60)

                          CGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGGCCTGGCCCGGCGGCGAGAACTACAGAATTGCGG                                                                    >  SRR4302537.6174131/1‑94 (MQ=60)

                           GTTATCAGGTTGAAACTCAGCGTCTTTACCATGTAATGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGA                                                                   >  SRR4302537.2216920/1‑94 (MQ=60)

                            TTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGAT                                                                  >  SRR4302537.1757159/1‑94 (MQ=60)

                            TTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTAGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGAT                                                                  <  SRR4302537.6026201/94‑1 (MQ=60)

                              ATGAGGTGGAACCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATAT                                                                <  SRR4302537.5317538/94‑1 (MQ=60)

                               TCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAGCTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATT                                                               <  SRR4302537.3401929/94‑1 (MQ=60)

                               TCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATT                                                               <  SRR4302537.6379165/94‑1 (MQ=60)

                                 AGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGC                                                             >  SRR4302537.5780292/1‑94 (MQ=60)

                                 AGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGC                                                             >  SRR4302537.4599924/1‑94 (MQ=60)

                                 AGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGC                                                             <  SRR4302537.2533151/94‑1 (MQ=60)

                                 AGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGC                                                             <  SRR4302537.4052191/94‑1 (MQ=60)

                                  GGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACacaggaattcgggatatggcc                                                            >  SRR4302537.4834281/1‑73 (MQ=60)

                                  GGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCC                                                            >  SRR4302537.3286021/1‑94 (MQ=60)

                                   GTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGGGGCGAGAACTACAGCATTGCGGATATTGCCT                                                           <  SRR4302537.4294342/94‑1 (MQ=60)

                                   GTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCT                                                           <  SRR4302537.2315238/94‑1 (MQ=60)

                                   GTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCT                                                           >  SRR4302537.1033331/1‑94 (MQ=60)

                                    TTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTG                                                          <  SRR4302537.6295512/94‑1 (MQ=60)

                                      GAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCT                                                        <  SRR4302537.5459694/94‑1 (MQ=60)

                                       AAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTG                                                       <  SRR4302537.5977338/94‑1 (MQ=60)

                                        AACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGG                                                      <  SRR4302537.1596098/94‑1 (MQ=60)

                                        AACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGG                                                      >  SRR4302537.3323214/1‑94 (MQ=60)

                                         ACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGC                                                     <  SRR4302537.3716010/94‑1 (MQ=60)

                                         ACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGC                                                     >  SRR4302537.4902731/1‑94 (MQ=60)

                                         ACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGC                                                     <  SRR4302537.5881704/94‑1 (MQ=60)

                                          CTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGTGGCGAGAACTACAGCATTGCTGATATTGCCTGCTGGCC                                                    >  SRR4302537.1159619/1‑94 (MQ=60)

                                          CTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCC                                                    <  SRR4302537.2340398/94‑1 (MQ=60)

                                          CTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCC                                                    >  SRR4302537.657262/1‑94 (MQ=60)

                                            CAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGT                                                  >  SRR4302537.4051218/1‑94 (MQ=60)

                                            CAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGG                                                  >  SRR4302537.1699820/1‑94 (MQ=60)

                                              GCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGG                                                >  SRR4302537.5312834/1‑94 (MQ=60)

                                              GCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGG                                                >  SRR4302537.2458737/1‑94 (MQ=60)

                                               CGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGG                                               <  SRR4302537.6024896/94‑1 (MQ=60)

                                               CGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGG                                               <  SRR4302537.489081/94‑1 (MQ=60)

                                                GTCTTTCCCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGT                                              <  SRR4302537.2746315/94‑1 (MQ=60)

                                                GTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGT                                              >  SRR4302537.5983467/1‑94 (MQ=60)

                                                GTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGT                                              >  SRR4302537.730836/1‑94 (MQ=60)

                                                GTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGT                                              >  SRR4302537.5797293/1‑94 (MQ=60)

                                                   TTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAA                                           <  SRR4302537.3210217/94‑1 (MQ=60)

                                                    TTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAT                                          <  SRR4302537.3586267/94‑1 (MQ=60)

                                                    TTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAT                                          >  SRR4302537.6412267/1‑94 (MQ=60)

                                                    TTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAT                                          <  SRR4302537.4341576/94‑1 (MQ=60)

                                                     TACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATG                                         <  SRR4302537.630487/94‑1 (MQ=60)

                                                     TACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATG                                         >  SRR4302537.6350833/1‑94 (MQ=60)

                                                       CCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCC                                       >  SRR4302537.4864918/1‑94 (MQ=60)

                                                       CCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCC                                       >  SRR4302537.2032643/1‑94 (MQ=60)

                                                       CCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCC                                       >  SRR4302537.2319101/1‑94 (MQ=60)

                                                        CATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCT                                      >  SRR4302537.919981/1‑94 (MQ=60)

                                                        CATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCT                                      <  SRR4302537.1565772/94‑1 (MQ=60)

                                                         ATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTG                                     <  SRR4302537.291563/94‑1 (MQ=60)

                                                          TGTCCTGAACAAGCGGCTGGAAATCTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGG                                    <  SRR4302537.3804947/94‑1 (MQ=60)

                                                            gagTGAACAAGCGGCTGGAACGCTCGCCCTGGCCGGGAGGCCAGAACTACAGCATTGCGGATATTGCATGCTGGCCGTGGGTTAATGCCTGGAC                                  <  SRR4302537.1877303/91‑1 (MQ=60)

                                                            TACTGAACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGAC                                  <  SRR4302537.5758638/94‑1 (MQ=60)

                                                                  ACAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCA                            <  SRR4302537.5750830/94‑1 (MQ=60)

                                                                   CAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAG                           <  SRR4302537.3797579/94‑1 (MQ=60)

                                                                   CAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAG                           <  SRR4302537.1594520/94‑1 (MQ=60)

                                                                   CAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAG                           <  SRR4302537.1162964/94‑1 (MQ=60)

                                                                   CAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTAGACTCGCCAG                           <  SRR4302537.438994/94‑1 (MQ=60)

                                                                   CAAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCATGCTGGCCGTGGGTTAATGCCTGGACTCGCCAG                           >  SRR4302537.4407097/1‑94 (MQ=60)

                                                                    AAGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGC                          >  SRR4302537.4796312/1‑94 (MQ=60)

                                                                     AGCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGAATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCG                         >  SRR4302537.4589042/1‑94 (MQ=60)

                                                                      GCGGCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGA                        <  SRR4302537.1485608/94‑1 (MQ=60)

                                                                         GCTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATT                     >  SRR4302537.4231767/1‑94 (MQ=60)

                                                                          CTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTG                    >  SRR4302537.2035654/1‑94 (MQ=60)

                                                                          CTGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTG                    <  SRR4302537.5992614/94‑1 (MQ=60)

                                                                           TGGAAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGTCTGGACTCGCCAGCGAATTGC                   >  SRR4302537.4954026/1‑94 (MQ=60)

                                                                              AAAACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCT                >  SRR4302537.4525467/1‑94 (MQ=60)

                                                                                 ACTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGGCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTCACCTAGC             >  SRR4302537.2403164/1‑94 (MQ=60)

                                                                                  CTCGCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCA            <  SRR4302537.1734531/94‑1 (MQ=60)

                                                                                     GCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATG         >  SRR4302537.1648495/1‑94 (MQ=60)

                                                                                     GCCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATG         <  SRR4302537.2989594/94‑1 (MQ=60)

                                                                                      CCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGT        >  SRR4302537.1753287/1‑94 (MQ=60)

                                                                                      CCCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGT        <  SRR4302537.5537931/94‑1 (MQ=60)

                                                                                       CCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTA       >  SRR4302537.3086692/1‑94 (MQ=60)

                                                                                       CCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTA       >  SRR4302537.4831664/1‑94 (MQ=60)

                                                                                       CCTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGAGAATTGACCTAGCAATGTA       >  SRR4302537.2175404/1‑94 (MQ=60)

                                                                                        CTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTAT      >  SRR4302537.2713189/1‑94 (MQ=60)

                                                                                        CTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTAT      <  SRR4302537.1554775/94‑1 (MQ=60)

                                                                                        CTGGCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTAT      <  SRR4302537.2812059/94‑1 (MQ=60)

                                                                                           GCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCG   <  SRR4302537.1764095/94‑1 (MQ=60)

                                                                                           GCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCG   >  SRR4302537.3510102/1‑94 (MQ=60)

                                                                                           GCCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCG   <  SRR4302537.2204179/94‑1 (MQ=60)

                                                                                           GCCGGGAGGCGAGAACTACAGCATTGCGCATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCG   <  SRR4302537.4591724/94‑1 (MQ=60)

                                                                                            CCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGG  <  SRR4302537.4795854/94‑1 (MQ=60)

                                                                                            CCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGG  <  SRR4302537.2898507/94‑1 (MQ=60)

                                                                                            CCGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGG  >  SRR4302537.937254/1‑94 (MQ=60)

CCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTCGCCAGCGAATTGACCTAGCAATGTATCCGG  >  NC_000913/2419008‑2419193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`