Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,456,917 C→T R88Q (CGG→CAG)  b2341 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,456,9170CT100.0% 704.0 / NA 197R88Q (CGG→CAG) b2341hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (110/87);  total (110/87)

Only 100 of 205 total aligned reads displayed.

TCCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAG  >  NC_000913/2456827‑2457006
                                                                                          |                                                                                         
 ccAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCtgcg                                                                                        >  2:2958823/1‑93 (MQ=255)
  cAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTgcgc                                                                                       >  1:5735558/1‑93 (MQ=255)
   aGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGcc                                                                                      >  1:1668833/1‑93 (MQ=255)
   aGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGcc                                                                                      >  2:42689/1‑93 (MQ=255)
     cccACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCag                                                                                    <  2:3088105/93‑1 (MQ=255)
       caccacCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCagag                                                                                  <  2:1871172/93‑1 (MQ=255)
        accaccCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGc                                                                                 >  1:1322229/1‑93 (MQ=255)
         ccaccCAGGCAGGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCt                                                                                <  1:2377650/93‑1 (MQ=255)
         ccaccCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCt                                                                                >  1:3162862/1‑93 (MQ=255)
          caccCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCtt                                                                               >  1:1872303/1‑93 (MQ=255)
          caccCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCtt                                                                               >  2:4718776/1‑93 (MQ=255)
          caccCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCACGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCtt                                                                               >  1:2315511/1‑93 (MQ=255)
           ccccAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTc                                                                              <  1:360681/92‑1 (MQ=255)
           accCTGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTc                                                                              <  2:1961531/93‑1 (MQ=255)
           accCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTc                                                                              >  2:2537257/1‑93 (MQ=255)
           accCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTc                                                                              <  1:621219/93‑1 (MQ=255)
              cAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGc                                                                           <  1:4376406/93‑1 (MQ=255)
               aGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGct                                                                          >  2:2983758/1‑93 (MQ=255)
                ggCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGctt                                                                         >  1:6094779/1‑93 (MQ=255)
                 gCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGcttc                                                                        <  2:769265/93‑1 (MQ=255)
                   aaGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGcttctt                                                                      <  2:5651849/93‑1 (MQ=255)
                    aGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTg                                                                     >  1:3687840/1‑93 (MQ=255)
                    aGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTg                                                                     >  2:2239005/1‑93 (MQ=255)
                    aGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTg                                                                     <  1:4417046/93‑1 (MQ=255)
                        ccATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGcc                                                                 >  2:3610713/1‑93 (MQ=255)
                        ccATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGcc                                                                 >  1:2510656/1‑93 (MQ=255)
                        ccATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGcc                                                                 >  1:1494954/1‑93 (MQ=255)
                            gAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGttt                                                             >  2:1751101/1‑93 (MQ=255)
                            gAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGttt                                                             <  2:1216690/93‑1 (MQ=255)
                             aaTAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGtttt                                                            >  1:3443621/1‑93 (MQ=255)
                              aTAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTg                                                           <  1:1687001/93‑1 (MQ=255)
                              aTAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTg                                                           <  1:1968849/93‑1 (MQ=255)
                               aaGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGc                                                          <  1:835408/92‑1 (MQ=255)
                                aGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCa                                                         <  2:4523230/93‑1 (MQ=255)
                                 gCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAg                                                        >  2:1292454/1‑93 (MQ=255)
                                 gCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAg                                                        >  2:4158787/1‑93 (MQ=255)
                                  ccGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGt                                                       <  1:5635773/93‑1 (MQ=255)
                                   cgcgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGtt                                                      <  2:3409139/93‑1 (MQ=255)
                                   cgcgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGtt                                                      <  1:1277602/93‑1 (MQ=255)
                                    gcgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTg                                                     >  1:4080532/1‑93 (MQ=255)
                                     cgATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGc                                                    <  1:205182/93‑1 (MQ=255)
                                      gATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGcc                                                   >  2:1446169/1‑93 (MQ=255)
                                      gATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGcc                                                   >  2:3343234/1‑93 (MQ=255)
                                       aTAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCg                                                  >  2:4887603/1‑93 (MQ=255)
                                       aTAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCg                                                  <  1:429457/93‑1 (MQ=255)
                                        tAACCTGAATGGGCAAAGCATGAATCTCCTCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGa                                                 <  2:5923943/93‑1 (MQ=255)
                                        tAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGa                                                 <  1:3204079/93‑1 (MQ=255)
                                         aaCCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGAt                                                >  1:5714563/1‑93 (MQ=255)
                                          aCCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATc                                               >  2:4195264/1‑93 (MQ=255)
                                           ccTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCa                                              <  2:4056462/93‑1 (MQ=255)
                                            cTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCAt                                             >  2:5976708/1‑93 (MQ=255)
                                            cTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCAt                                             >  1:1408227/1‑93 (MQ=255)
                                             tGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATg                                            >  2:5297364/1‑93 (MQ=255)
                                             tGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATg                                            <  1:4747972/93‑1 (MQ=255)
                                             tGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGAGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATg                                            >  2:2663392/1‑93 (MQ=255)
                                              gAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGt                                           <  2:3924743/93‑1 (MQ=255)
                                              gAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCACTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGt                                           <  1:5158245/93‑1 (MQ=255)
                                               aaTGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGtt                                          >  2:1651519/1‑93 (MQ=255)
                                                aTGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTg                                         >  2:3062176/1‑93 (MQ=255)
                                                aTGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTg                                         >  1:5393360/1‑93 (MQ=255)
                                                 tGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGa                                        <  1:2792925/93‑1 (MQ=255)
                                                 tGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGa                                        >  1:306233/1‑93 (MQ=255)
                                                 tGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGa                                        >  1:4521056/1‑93 (MQ=255)
                                                   ggCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATg                                      >  2:523469/1‑93 (MQ=255)
                                                    gCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGt                                     <  1:5546582/93‑1 (MQ=255)
                                                         gCATGAATCACCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTgtg                                >  1:6470449/1‑91 (MQ=255)
                                                            tGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCa                             >  1:5018843/1‑93 (MQ=255)
                                                            tGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCa                             >  1:5265961/1‑93 (MQ=255)
                                                             gAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAg                            >  1:4200509/1‑93 (MQ=255)
                                                              aaTCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGc                           <  1:5954733/93‑1 (MQ=255)
                                                                tctcCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCaa                         <  2:567424/93‑1 (MQ=255)
                                                                tctcCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCaa                         >  2:3287881/1‑93 (MQ=255)
                                                                 ctcCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAAt                        <  1:3717756/93‑1 (MQ=255)
                                                                  tcCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATg                       >  1:4241896/1‑93 (MQ=255)
                                                                  tcCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATg                       >  2:2809321/1‑93 (MQ=255)
                                                                   ccGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGa                      <  2:6254449/93‑1 (MQ=255)
                                                                   ccGCCATCAACTGTTGGCCCTGCTGCACCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCNTGTTGATGTCTGCGCCAGCAATGa                      >  2:1305918/1‑93 (MQ=255)
                                                                      ccATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGt                   >  2:4988301/1‑93 (MQ=255)
                                                                      ccATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGt                   >  2:6307305/1‑93 (MQ=255)
                                                                      ccATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGt                   >  1:6383002/1‑93 (MQ=255)
                                                                       cATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGtt                  >  2:2118193/1‑93 (MQ=255)
                                                                        aTCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTg                 <  2:2148023/93‑1 (MQ=255)
                                                                        aTCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTg                 <  2:4800376/93‑1 (MQ=255)
                                                                        aTCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTg                 <  2:313189/93‑1 (MQ=255)
                                                                         tCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGt                >  1:1456709/1‑93 (MQ=255)
                                                                         tCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGACAGCAATGAAGTTGt                >  1:1607611/1‑93 (MQ=255)
                                                                            aCTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCg             >  1:875811/1‑93 (MQ=255)
                                                                             cTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCgg            <  2:3795356/93‑1 (MQ=255)
                                                                             cTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCgg            <  1:254981/93‑1 (MQ=255)
                                                                             cTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCgg            >  1:976277/1‑93 (MQ=255)
                                                                              tGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGt           <  1:3989992/93‑1 (MQ=255)
                                                                              tGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGt           <  2:6055444/93‑1 (MQ=255)
                                                                              tGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGt           <  1:5142446/93‑1 (MQ=255)
                                                                                ttGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGttt         >  1:1804893/1‑93 (MQ=255)
                                                                                ttGGCCCTGCTGCGCCAGAGC‑TCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTa        >  1:5797621/1‑93 (MQ=255)
                                                                                   gCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGc      >  2:5590715/1‑93 (MQ=255)
                                                                                   gCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGc      >  1:2689576/1‑93 (MQ=255)
                                                                                     ccTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCgg    >  1:3650626/1‑93 (MQ=255)
                                                                                      ctgctgCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGga   <  1:1086820/93‑1 (MQ=255)
                                                                                       tgctgcGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGgag  <  2:5065278/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAG  >  NC_000913/2456827‑2457006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTCCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAA  >  NC_000913/2456824‑2457010
                                                                                             |                                                                                             
AACTCCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCT                                                                                               <  SRR4302537.1641115/94‑1 (MQ=60)
 ACTCCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTG                                                                                              >  SRR4302537.3810513/1‑94 (MQ=60)
  CTCCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGC                                                                                             <  SRR4302537.1531243/94‑1 (MQ=60)
   TCCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCG                                                                                            <  SRR4302537.5597404/94‑1 (MQ=60)
    CCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGC                                                                                           >  SRR4302537.5774827/1‑94 (MQ=60)
     CAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCC                                                                                          >  SRR4302537.1680524/1‑94 (MQ=60)
     CAGACCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCC                                                                                          >  SRR4302537.1322420/1‑94 (MQ=60)
      AGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCA                                                                                         <  SRR4302537.2352144/94‑1 (MQ=60)
          CACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGC                                                                                     >  SRR4302537.1331443/1‑94 (MQ=60)
           CCCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCT                                                                                    <  SRR4302537.5711372/94‑1 (MQ=60)
           ACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCT                                                                                    >  SRR4302537.3185068/1‑94 (MQ=60)
            CCACCCAGGCAGGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTT                                                                                   <  SRR4302537.2394404/94‑1 (MQ=60)
            CCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTT                                                                                   >  SRR4302537.1885333/1‑94 (MQ=60)
            CCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTT                                                                                   >  SRR4302537.4364471/1‑94 (MQ=60)
            CCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCACGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTT                                                                                   >  SRR4302537.2331857/1‑94 (MQ=60)
            CCACCCAGGCAAGCGCAATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTT                                                                                   >  SRR4302537.3670270/1‑94 (MQ=60)
              CCCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCC                                                                                 <  SRR4302537.363189/94‑1 (MQ=60)
              ACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCC                                                                                 >  SRR4302537.315355/1‑94 (MQ=60)
              ACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCC                                                                                 <  SRR4302537.625450/94‑1 (MQ=60)
               CCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCG                                                                                <  SRR4302537.5212207/94‑1 (MQ=60)
                 CAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCT                                                                              <  SRR4302537.4406628/94‑1 (MQ=60)
                 CAGGCAAGCGCCATGAATAGCCGCGATAACCGGAATGGTCAAAGCATGAATCCCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCT                                                                              <  SRR4302537.991239/94‑1 (MQ=60)
                  AGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTT                                                                             >  SRR4302537.625535/1‑94 (MQ=60)
                  AGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTT                                                                             >  SRR4302537.6136659/1‑94 (MQ=60)
                     CAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTT                                                                          <  SRR4302537.4300057/94‑1 (MQ=60)
                      AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTG                                                                         >  SRR4302537.3713776/1‑94 (MQ=60)
                      AAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTG                                                                         <  SRR4302537.1727367/94‑1 (MQ=60)
                       AGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGC                                                                        <  SRR4302537.4447537/94‑1 (MQ=60)
                         CGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGC                                                                      <  SRR4302537.4191103/94‑1 (MQ=60)
                          GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCC                                                                     >  SRR4302537.2528315/1‑94 (MQ=60)
                          GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCC                                                                     >  SRR4302537.2528894/1‑94 (MQ=60)
                          GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCC                                                                     >  SRR4302537.1350388/1‑94 (MQ=60)
                          GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCC                                                                     >  SRR4302537.1505374/1‑94 (MQ=60)
                          GCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCC                                                                     >  SRR4302537.2294957/1‑94 (MQ=60)
                             ATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCACCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTT                                                                  >  SRR4302537.3492519/1‑94 (MQ=60)
                               GAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTT                                                                >  SRR4302537.3467985/1‑94 (MQ=60)
                                AATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTG                                                               >  SRR4302537.5332226/1‑94 (MQ=60)
                                 ATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGC                                                              <  SRR4302537.1698815/94‑1 (MQ=60)
                                 ATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGC                                                              <  SRR4302537.1982542/94‑1 (MQ=60)
                                  AAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCA                                                             <  SRR4302537.841123/94‑1 (MQ=60)
                                     CCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTT                                                          <  SRR4302537.5674341/94‑1 (MQ=60)
                                     CCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTT                                                          >  SRR4302537.4256329/1‑94 (MQ=60)
                                      CGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTG                                                         >  SRR4302537.4109016/1‑94 (MQ=60)
                                      CGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTG                                                         >  SRR4302537.6169005/1‑94 (MQ=60)
                                      CGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTG                                                         <  SRR4302537.1286478/94‑1 (MQ=60)
                                        CGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCC                                                       <  SRR4302537.206654/94‑1 (MQ=60)
                                          ATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGA                                                     >  SRR4302537.5405777/1‑94 (MQ=60)
                                          ATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGA                                                     <  SRR4302537.432451/94‑1 (MQ=60)
                                           TAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGAT                                                    <  SRR4302537.3226603/94‑1 (MQ=60)
                                           TAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGAT                                                    >  SRR4302537.5753670/1‑94 (MQ=60)
                                            AACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATC                                                   >  SRR4302537.5833665/1‑94 (MQ=60)
                                             ACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCA                                                  >  SRR4302537.1692621/1‑94 (MQ=60)
                                              CCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCAT                                                 >  SRR4302537.1418034/1‑94 (MQ=60)
                                               CTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATG                                                <  SRR4302537.966911/94‑1 (MQ=60)
                                                TGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGT                                               >  SRR4302537.3386674/1‑94 (MQ=60)
                                                TGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGT                                               >  SRR4302537.3134230/1‑94 (MQ=60)
                                                TGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGT                                               <  SRR4302537.4780772/94‑1 (MQ=60)
                                                 GAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTT                                              <  SRR4302537.881596/94‑1 (MQ=60)
                                                 GAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCACTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTT                                              <  SRR4302537.5193789/94‑1 (MQ=60)
                                                  AATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTG                                             >  SRR4302537.5430196/1‑94 (MQ=60)
                                                   ATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGA                                            >  SRR4302537.308354/1‑94 (MQ=60)
                                                   ATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGA                                            >  SRR4302537.4552327/1‑94 (MQ=60)
                                                    TGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGAT                                           <  SRR4302537.2812443/94‑1 (MQ=60)
                                                       GCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTC                                        >  SRR4302537.3697685/1‑94 (MQ=60)
                                                       GCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTC                                        <  SRR4302537.5584457/94‑1 (MQ=60)
                                                        CAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCT                                       >  SRR4302537.720511/1‑94 (MQ=60)
                                                          AAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGC                                     <  SRR4302537.4418941/94‑1 (MQ=60)
                                                           AGCATGAATCACCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGTG                                    >  SRR4302537.6514316/1‑94 (MQ=60)
                                                             CATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCCCC                                  >  SRR4302537.1506811/1‑94 (MQ=60)
                                                              ATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCA                                 >  SRR4302537.2621938/1‑94 (MQ=60)
                                                              ATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCA                                 >  SRR4302537.5302196/1‑94 (MQ=60)
                                                              ATGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCA                                 >  SRR4302537.5053414/1‑94 (MQ=60)
                                                               acAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAG                                <  SRR4302537.4023695/92‑1 (MQ=60)
                                                               TGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAG                                <  SRR4302537.6487836/94‑1 (MQ=60)
                                                               TGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAG                                >  SRR4302537.4229837/1‑94 (MQ=60)
                                                               TGAATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAG                                >  SRR4302537.190755/1‑94 (MQ=60)
                                                                 AATCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCA                              <  SRR4302537.5995610/94‑1 (MQ=60)
                                                                   TCTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAAT                            <  SRR4302537.1850286/94‑1 (MQ=60)
                                                                    CTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATG                           <  SRR4302537.113143/94‑1 (MQ=60)
                                                                    CTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATG                           >  SRR4302537.4271389/1‑94 (MQ=60)
                                                                    CTCCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATG                           <  SRR4302537.3743886/94‑1 (MQ=60)
                                                                      CCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAA                         <  SRR4302537.2787544/94‑1 (MQ=60)
                                                                      CCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAA                         <  SRR4302537.5937059/94‑1 (MQ=60)
                                                                      CCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAA                         <  SRR4302537.5999898/94‑1 (MQ=60)
                                                                      CCGCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAA                         <  SRR4302537.2438497/94‑1 (MQ=60)
                                                                        GCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGT                       >  SRR4302537.5189961/1‑94 (MQ=60)
                                                                        GCCATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGT                       >  SRR4302537.6426512/1‑94 (MQ=60)
                                                                          CATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTG                     <  SRR4302537.3960051/94‑1 (MQ=60)
                                                                           ATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGT                    >  SRR4302537.1466856/1‑94 (MQ=60)
                                                                           ATCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGACAGCAATGAAGTTGT                    >  SRR4302537.1618852/1‑94 (MQ=60)
                                                                            TCAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTC                   <  SRR4302537.2851873/94‑1 (MQ=60)
                                                                             CAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCC                  <  SRR4302537.5528640/94‑1 (MQ=60)
                                                                             CAACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCC                  <  SRR4302537.4551039/94‑1 (MQ=60)
                                                                              AACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCG                 >  SRR4302537.881832/1‑94 (MQ=60)
                                                                              AACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCG                 <  SRR4302537.1430118/94‑1 (MQ=60)
                                                                               ACTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGG                >  SRR4302537.983010/1‑94 (MQ=60)
                                                                                CTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGT               <  SRR4302537.256790/94‑1 (MQ=60)
                                                                                CTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGT               >  SRR4302537.5133771/1‑94 (MQ=60)
                                                                                CTGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTACGCCAGCAATGAAGTTGTCCGGT               >  SRR4302537.1646394/1‑94 (MQ=60)
                                                                                 TGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTT              <  SRR4302537.5177882/94‑1 (MQ=60)
                                                                                 TGTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTT              <  SRR4302537.4017930/94‑1 (MQ=60)
                                                                                 TGTTGGCACTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTT              >  SRR4302537.3714908/1‑94 (MQ=60)
                                                                                  GTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTT             >  SRR4302537.4963689/1‑94 (MQ=60)
                                                                                  GTTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTT             >  SRR4302537.1817488/1‑94 (MQ=60)
                                                                                  GTTGGCCCTGCTGCGCCAGAGC‑TCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTA            >  SRR4302537.5837367/1‑94 (MQ=60)
                                                                                   TTGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTA            <  SRR4302537.5756581/94‑1 (MQ=60)
                                                                                    TGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAG           <  SRR4302537.2065121/94‑1 (MQ=60)
                                                                                    TGGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAG           >  SRR4302537.4727693/1‑94 (MQ=60)
                                                                                     GGCCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGC          >  SRR4302537.2708346/1‑94 (MQ=60)
                                                                                     GGCCCTGCTGCGCCAGAGCGTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGC          <  SRR4302537.1490065/94‑1 (MQ=60)
                                                                                       CCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGG        >  SRR4302537.3219475/1‑94 (MQ=60)
                                                                                       CCCTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGG        >  SRR4302537.3676326/1‑94 (MQ=60)
                                                                                         CTGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAG      <  SRR4302537.1094324/94‑1 (MQ=60)
                                                                                          TGCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGA     >  SRR4302537.5958115/1‑94 (MQ=60)
                                                                                           GCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGAC    <  SRR4302537.4779683/94‑1 (MQ=60)
                                                                                           GCTGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGAC    <  SRR4302537.5074207/94‑1 (MQ=60)
                                                                                             TGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAA  >  SRR4302537.5939834/1‑94 (MQ=60)
                                                                                             |                                                                                             
AACTCCAGCCCACCACCCAGGCAAGCGCCATGAATAGCCGCGATAACCTGAATGGGCAAAGCATGAATCTCCGCCATCAACTGTTGGCCCTGCCGCGCCAGAGCTTCCGCTTCTTGCGCCGTTTTGCAGTTGCCGATCATGTTGATGTCTGCGCCAGCAATGAAGTTGTCCGGTTTAGCGGAGACAA  >  NC_000913/2456824‑2457010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑