Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,475,572 A→G A26A (GCT→GCC)  dsdC ← D‑serine dehydratase (deaminase) transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,475,5720AG100.0% 915.1 / NA 264A26A (GCT→GCC) dsdCD‑serine dehydratase (deaminase) transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (141/123);  total (141/123)

Only 100 of 276 total aligned reads displayed.

TTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATT  >  NC_000913/2475482‑2475661
                                                                                          |                                                                                         
ttcttcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTggcggc                                                                                         >  1:6262879/1‑93 (MQ=255)
 tcttcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGcc                                                                                        <  2:1099076/93‑1 (MQ=255)
 tcttcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGcc                                                                                        >  1:775249/1‑93 (MQ=255)
  cttcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCa                                                                                       >  2:1549660/1‑93 (MQ=255)
  cttcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCa                                                                                       >  1:1452743/1‑93 (MQ=255)
  cttcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCa                                                                                       >  2:2736399/1‑93 (MQ=255)
   ttcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCAc                                                                                      <  2:1252303/93‑1 (MQ=255)
    tcttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACt                                                                                     >  2:4768627/1‑93 (MQ=255)
     cttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACtt                                                                                    <  1:2226478/93‑1 (MQ=255)
      ttcCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTc                                                                                   >  2:4387975/1‑93 (MQ=255)
         cAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCaaa                                                                                >  2:813233/1‑93 (MQ=255)
         cAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCaaa                                                                                >  2:1196842/1‑93 (MQ=255)
          aGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCaaaa                                                                               <  1:5423023/93‑1 (MQ=255)
          aGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCaaaa                                                                               >  1:467839/1‑93 (MQ=255)
           gCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAg                                                                              >  2:4021443/1‑93 (MQ=255)
           gCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAg                                                                              >  2:2799983/1‑93 (MQ=255)
           gCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAg                                                                              >  1:6204419/1‑93 (MQ=255)
           gCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAg                                                                              >  2:2450506/1‑93 (MQ=255)
             tgattgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTa                                                                            <  1:6149181/93‑1 (MQ=255)
             tgattgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTa                                                                            <  2:625438/93‑1 (MQ=255)
             tgattgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTa                                                                            >  1:1742848/1‑93 (MQ=255)
              gattgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTAt                                                                           >  2:4624190/1‑93 (MQ=255)
              gattgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTAt                                                                           >  2:3170150/1‑93 (MQ=255)
               attgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATg                                                                          >  2:42736/1‑93 (MQ=255)
               attgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATg                                                                          >  1:5185389/1‑93 (MQ=255)
                ttgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGc                                                                         <  2:2585652/93‑1 (MQ=255)
                   atACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCAtt                                                                      >  1:4515497/1‑93 (MQ=255)
                    tACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCAttt                                                                     >  1:3509004/1‑93 (MQ=255)
                     aCGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCAtttt                                                                    <  2:2872506/93‑1 (MQ=255)
                     aCGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCAtttt                                                                    <  1:6410764/93‑1 (MQ=255)
                     aCGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCAtttt                                                                    <  2:3123095/93‑1 (MQ=255)
                     aCGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCAtttt                                                                    <  1:2786718/93‑1 (MQ=255)
                      cGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCAttttt                                                                   >  2:4211835/1‑93 (MQ=255)
                         tGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGAt                                                                >  1:1022991/1‑93 (MQ=255)
                          gACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATa                                                               >  1:2393831/1‑93 (MQ=255)
                           aCTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATaa                                                              <  2:4469788/93‑1 (MQ=255)
                           aCTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATaa                                                              <  1:5353076/93‑1 (MQ=255)
                            cTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAAt                                                             >  1:4415170/1‑93 (MQ=255)
                            cTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGACTGGCGGCCACTTCAAAACTATGCATTTTTCATAAt                                                             >  2:5253143/1‑93 (MQ=255)
                             ttACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAAtt                                                            <  1:2688453/93‑1 (MQ=255)
                              tACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTg                                                           >  2:3804073/1‑93 (MQ=255)
                                ccGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGcc                                                         >  2:2239178/1‑93 (MQ=255)
                                 cGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCa                                                        <  2:2101773/93‑1 (MQ=255)
                                 cGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCa                                                        <  1:5014394/93‑1 (MQ=255)
                                 cGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCa                                                        <  2:2293017/93‑1 (MQ=255)
                                 cGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCa                                                        >  2:1318288/1‑93 (MQ=255)
                                 cGCACTGGGGCCCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCa                                                        <  2:2695278/93‑1 (MQ=255)
                                  gCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAg                                                       <  2:4829931/93‑1 (MQ=255)
                                  gCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAg                                                       >  2:2846926/1‑93 (MQ=255)
                                  gCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGAGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAg                                                       >  2:2958549/1‑93 (MQ=255)
                                      tGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGt                                                   >  1:927398/1‑93 (MQ=255)
                                       ggggCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGtt                                                  >  1:5935005/1‑93 (MQ=255)
                                        gggCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTa                                                 <  1:2521210/93‑1 (MQ=255)
                                         ggCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTaa                                                >  1:1292/1‑93 (MQ=255)
                                              aGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAagcagc                                           <  2:5468994/93‑1 (MQ=255)
                                               gCGACACTTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCGCTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGcc                                          <  1:3742838/93‑1 (MQ=255)
                                               gCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGGTAAGCAGcc                                          >  2:6047601/1‑93 (MQ=255)
                                               gCGACAATCCCTCTGCCGCCAGGGCGAAGTACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGcc                                          <  2:3426602/93‑1 (MQ=255)
                                                  aCAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGAt                                       >  1:4920989/1‑93 (MQ=255)
                                                    aaTTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGAttt                                     >  1:3996196/1‑93 (MQ=255)
                                                     aTTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTc                                    >  2:6336765/1‑93 (MQ=255)
                                                     aTTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTc                                    >  2:2157605/1‑93 (MQ=255)
                                                         ctctGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAt                                >  2:1722609/1‑93 (MQ=255)
                                                         ctctGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAt                                >  1:3625190/1‑93 (MQ=255)
                                                          tctGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAtt                               >  1:3349042/1‑93 (MQ=255)
                                                          tctGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAtt                               <  1:563585/93‑1 (MQ=255)
                                                          tctGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAtt                               >  2:3979271/1‑93 (MQ=255)
                                                          tctGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAtt                               >  1:997939/1‑93 (MQ=255)
                                                           ctGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAttt                              <  2:3402942/93‑1 (MQ=255)
                                                            tGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTc                             <  1:5030408/93‑1 (MQ=255)
                                                             gcccccAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCa                            <  1:3190561/93‑1 (MQ=255)
                                                               cgccAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACg                          >  2:559170/1‑93 (MQ=255)
                                                                 ccAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGaa                        <  2:1417694/93‑1 (MQ=255)
                                                                 ccAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGaa                        >  1:5265405/1‑93 (MQ=255)
                                                                  cAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAg                       >  2:1968217/1‑93 (MQ=255)
                                                                   aGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAgg                      >  2:367204/1‑93 (MQ=255)
                                                                   aGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAgg                      <  2:2775983/93‑1 (MQ=255)
                                                                   aGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTCCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAgg                      <  1:4195605/93‑1 (MQ=255)
                                                                    gggCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAggg                     >  2:3244083/1‑93 (MQ=255)
                                                                    gggCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAggg                     <  1:1183007/93‑1 (MQ=255)
                                                                    gggCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAggg                     >  1:1607959/1‑93 (MQ=255)
                                                                    gggCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAggg                     >  1:2252214/1‑93 (MQ=255)
                                                                    gggCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAggg                     >  2:5944809/1‑93 (MQ=255)
                                                                    gggCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAggg                     <  2:4251547/93‑1 (MQ=255)
                                                                     ggCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAgggg                    >  1:737242/1‑93 (MQ=255)
                                                                      gCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGt                   <  2:6416470/93‑1 (MQ=255)
                                                                      gCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATAGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGt                   <  1:3032145/93‑1 (MQ=255)
                                                                       cGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGtt                  <  1:2005728/93‑1 (MQ=255)
                                                                          aGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCa               >  1:851133/1‑93 (MQ=255)
                                                                          aGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCa               >  2:3566356/1‑93 (MQ=255)
                                                                           ggACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCAt              <  2:5749877/93‑1 (MQ=255)
                                                                             aCTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATaa            >  2:3009956/1‑93 (MQ=255)
                                                                              cTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAAt           >  1:3895281/1‑93 (MQ=255)
                                                                               tGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAAta          >  1:4276851/1‑92 (MQ=255)
                                                                                 aTGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCAc        >  2:1836518/1‑93 (MQ=255)
                                                                                  tGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCAcc       <  1:2902357/93‑1 (MQ=255)
                                                                                     cTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCa    >  2:4996195/1‑93 (MQ=255)
                                                                                      tGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAt   <  1:2572538/93‑1 (MQ=255)
                                                                                      tGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAt   >  2:1581910/1‑93 (MQ=255)
                                                                                      tGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAt   >  2:3351288/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATT  >  NC_000913/2475482‑2475661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTC  >  NC_000913/2475479‑2475665
                                                                                             |                                                                                             
CAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCG                                                                                               <  SRR4302537.4817201/94‑1 (MQ=60)
  ATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGC                                                                                             >  SRR4302537.6305872/1‑94 (MQ=60)
   TTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCC                                                                                            <  SRR4302537.2703417/94‑1 (MQ=60)
   TTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCC                                                                                            >  SRR4302537.780561/1‑94 (MQ=60)
    TCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCA                                                                                           <  SRR4302537.6437050/94‑1 (MQ=60)
    TCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCA                                                                                           >  SRR4302537.1462868/1‑94 (MQ=60)
      TTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACT                                                                                         <  SRR4302537.3382103/94‑1 (MQ=60)
        CTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTC                                                                                       <  SRR4302537.2242182/94‑1 (MQ=60)
         TCCCAGATGATTAATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCA                                                                                      <  SRR4302537.4014874/94‑1 (MQ=60)
           CCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAA                                                                                    >  SRR4302537.5678356/1‑94 (MQ=60)
           CCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAA                                                                                    >  SRR4302537.5839039/1‑94 (MQ=60)
            CAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAA                                                                                   <  SRR4302537.3966823/94‑1 (MQ=60)
            CAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAA                                                                                   >  SRR4302537.471051/1‑94 (MQ=60)
            CAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAA                                                                                   >  SRR4302537.1906648/1‑94 (MQ=60)
             AGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAG                                                                                  >  SRR4302537.6247180/1‑94 (MQ=60)
             AGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAG                                                                                  <  SRR4302537.5459989/94‑1 (MQ=60)
             AGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAG                                                                                  >  SRR4302537.1367936/1‑94 (MQ=60)
              GCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGT                                                                                 <  SRR4302537.5047528/94‑1 (MQ=60)
              GCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGT                                                                                 >  SRR4302537.4408646/1‑94 (MQ=60)
               CTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTA                                                                                >  SRR4302537.627012/1‑94 (MQ=60)
               CTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTA                                                                                <  SRR4302537.4527362/94‑1 (MQ=60)
               CTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTA                                                                                <  SRR4302537.3116501/94‑1 (MQ=60)
               CTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTA                                                                                >  SRR4302537.1754998/1‑94 (MQ=60)
                TGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTAT                                                                               >  SRR4302537.4715720/1‑94 (MQ=60)
                TGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTAT                                                                               <  SRR4302537.6191472/94‑1 (MQ=60)
                 GATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATG                                                                              >  SRR4302537.5221057/1‑94 (MQ=60)
                  ATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGC                                                                             >  SRR4302537.4559190/1‑94 (MQ=60)
                   TTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCA                                                                            >  SRR4302537.4106703/1‑94 (MQ=60)
                   TTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCA                                                                            <  SRR4302537.1588690/94‑1 (MQ=60)
                    TGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCAT                                                                           >  SRR4302537.5428602/1‑94 (MQ=60)
                     GATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATT                                                                          >  SRR4302537.4546726/1‑94 (MQ=60)
                      ATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTT                                                                         >  SRR4302537.3533831/1‑94 (MQ=60)
                        ACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTT                                                                       <  SRR4302537.2806203/94‑1 (MQ=60)
                        ACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTT                                                                       <  SRR4302537.6454381/94‑1 (MQ=60)
                        ACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTACGCATTTTT                                                                       >  SRR4302537.3820587/1‑94 (MQ=60)
                         CGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTG                                                                      <  SRR4302537.5378612/94‑1 (MQ=60)
                         CGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTG                                                                      >  SRR4302537.4666785/1‑94 (MQ=60)
                           GTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGAT                                                                    >  SRR4302537.1030033/1‑94 (MQ=60)
                           GTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGAT                                                                    <  SRR4302537.6035925/94‑1 (MQ=60)
                            TGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATA                                                                   >  SRR4302537.2410692/1‑94 (MQ=60)
                              ACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAAT                                                                 <  SRR4302537.5121276/94‑1 (MQ=60)
                              ACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAAT                                                                 >  SRR4302537.4445651/1‑94 (MQ=60)
                              ACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAAT                                                                 >  SRR4302537.1210656/1‑94 (MQ=60)
                              ACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAAT                                                                 >  SRR4302537.6491119/1‑94 (MQ=60)
                              ACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAAT                                                                 <  SRR4302537.5389739/94‑1 (MQ=60)
                              ACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAAT                                                                 >  SRR4302537.2125840/1‑94 (MQ=60)
                               CTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATT                                                                <  SRR4302537.2254735/94‑1 (MQ=60)
                                TTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTG                                                               <  SRR4302537.2707215/94‑1 (MQ=60)
                                 TACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGC                                                              >  SRR4302537.5345924/1‑94 (MQ=60)
                                  ACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCC                                                             >  SRR4302537.1170389/1‑94 (MQ=60)
                                   CCGCACTGGGGCTCAGCGGCAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCA                                                            <  SRR4302537.86218/94‑1 (MQ=60)
                                    CGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAG                                                           <  SRR4302537.5048932/94‑1 (MQ=60)
                                     GCACGGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACGGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGC                                                          <  SRR4302537.1778531/94‑1 (MQ=60)
                                      CACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCC                                                         >  SRR4302537.1306389/1‑94 (MQ=60)
                                      CACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCC                                                         >  SRR4302537.872261/1‑94 (MQ=60)
                                        CTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGT                                                       >  SRR4302537.933790/1‑94 (MQ=60)
                                         TGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTT                                                      >  SRR4302537.5975734/1‑94 (MQ=60)
                                           GGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAA                                                    <  SRR4302537.1647844/94‑1 (MQ=60)
                                           GGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAA                                                    <  SRR4302537.2538910/94‑1 (MQ=60)
                                           GGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAA                                                    <  SRR4302537.4249141/94‑1 (MQ=60)
                                           GGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAA                                                    >  SRR4302537.1300/1‑94 (MQ=60)
                                             GCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGC                                                  <  SRR4302537.2149569/94‑1 (MQ=60)
                                             GCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGC                                                  >  SRR4302537.2918664/1‑94 (MQ=60)
                                              CTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCA                                                 >  SRR4302537.5059287/1‑94 (MQ=60)
                                               TCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAG                                                >  SRR4302537.3124336/1‑94 (MQ=60)
                                                  GCGACACTTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCGCTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCG                                             <  SRR4302537.3769121/94‑1 (MQ=60)
                                                  GCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCG                                             >  SRR4302537.806087/1‑94 (MQ=60)
                                                    GACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGAT                                           <  SRR4302537.300473/94‑1 (MQ=60)
                                                    GACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGAT                                           >  SRR4302537.4954934/1‑94 (MQ=60)
                                                     ACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATT                                          >  SRR4302537.6079694/1‑94 (MQ=60)
                                                     ACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATT                                          >  SRR4302537.5561184/1‑94 (MQ=60)
                                                      CAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTT                                         >  SRR4302537.4024172/1‑94 (MQ=60)
                                                       AATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTC                                        <  SRR4302537.5266704/94‑1 (MQ=60)
                                                       AATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTC                                        >  SRR4302537.5575190/1‑94 (MQ=60)
                                                       AATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTC                                        <  SRR4302537.3754238/94‑1 (MQ=60)
                                                        ATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCT                                       >  SRR4302537.3497926/1‑94 (MQ=60)
                                                          TCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTA                                     >  SRR4302537.1543040/1‑94 (MQ=60)
                                                          TCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTA                                     <  SRR4302537.4849236/94‑1 (MQ=60)
                                                          TCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTA                                     >  SRR4302537.2578827/1‑94 (MQ=60)
                                                          TCCTCTGCCGCCAGGGCAAAGGACTGATGCCTGGCGGCCAGTTCAAAAGTATGCTTTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTA                                     <  SRR4302537.6416009/94‑1 (MQ=60)
                                                           CCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAT                                    >  SRR4302537.3650737/1‑94 (MQ=60)
                                                            CTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATT                                   >  SRR4302537.1004823/1‑94 (MQ=60)
                                                            CTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATT                                   >  SRR4302537.3372749/1‑94 (MQ=60)
                                                             TCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTT                                  <  SRR4302537.5017944/94‑1 (MQ=60)
                                                             TCTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTT                                  <  SRR4302537.567461/94‑1 (MQ=60)
                                                              CTGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTC                                 <  SRR4302537.2430866/94‑1 (MQ=60)
                                                               TGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCA                                <  SRR4302537.6256497/94‑1 (MQ=60)
                                                               TGCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCA                                <  SRR4302537.5065042/94‑1 (MQ=60)
                                                                GCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCAC                               <  SRR4302537.717478/94‑1 (MQ=60)
                                                                GCCGCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCAC                               >  SRR4302537.2652038/1‑94 (MQ=60)
                                                                GCCCCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCAC                               <  SRR4302537.3212998/94‑1 (MQ=60)
                                                                   GCCAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAA                            >  SRR4302537.5301636/1‑94 (MQ=60)
                                                                     CAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGG                          <  SRR4302537.4165590/94‑1 (MQ=60)
                                                                     CAGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGG                          >  SRR4302537.2532785/1‑94 (MQ=60)
                                                                      AGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGG                         >  SRR4302537.6242968/1‑94 (MQ=60)
                                                                      AGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGG                         >  SRR4302537.1619201/1‑94 (MQ=60)
                                                                      AGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGG                         >  SRR4302537.2268112/1‑94 (MQ=60)
                                                                      AGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGG                         >  SRR4302537.3555433/1‑94 (MQ=60)
                                                                      AGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGG                         >  SRR4302537.1935364/1‑94 (MQ=60)
                                                                      AGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGG                         <  SRR4302537.2254860/94‑1 (MQ=60)
                                                                      AGGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTCCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGG                         <  SRR4302537.4224908/94‑1 (MQ=60)
                                                                       GGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGG                        <  SRR4302537.3681619/94‑1 (MQ=60)
                                                                       GGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGG                        >  SRR4302537.742268/1‑94 (MQ=60)
                                                                       GGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGG                        <  SRR4302537.762338/94‑1 (MQ=60)
                                                                       GGGCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGG                        <  SRR4302537.1191235/94‑1 (MQ=60)
                                                                         GCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTT                      <  SRR4302537.1001528/94‑1 (MQ=60)
                                                                         GCGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATAGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTT                      <  SRR4302537.3053287/94‑1 (MQ=60)
                                                                          CGAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTC                     <  SRR4302537.2019696/94‑1 (MQ=60)
                                                                           GAAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCC                    <  SRR4302537.5098078/94‑1 (MQ=60)
                                                                            AAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTTACGAAGGGGTTCCA                   >  SRR4302537.995049/1‑94 (MQ=60)
                                                                            AAGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCA                   >  SRR4302537.856967/1‑94 (MQ=60)
                                                                             AGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCTGCCGTTAAGCAGCAGATTTCTTATTTCACGCAGGGGTTCCAT                  >  SRR4302537.3544007/1‑94 (MQ=60)
                                                                             AGGACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCAT                  <  SRR4302537.1196763/94‑1 (MQ=60)
                                                                                ACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAAT               >  SRR4302537.3347494/1‑94 (MQ=60)
                                                                                ACTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAAT               >  SRR4302537.3922512/1‑94 (MQ=60)
                                                                                 CTGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATA              >  SRR4302537.4306571/1‑94 (MQ=60)
                                                                                  TGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCA             <  SRR4302537.2757093/94‑1 (MQ=60)
                                                                                  TGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCA             >  SRR4302537.3048764/1‑94 (MQ=60)
                                                                                  TGATGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCA             <  SRR4302537.43055/94‑1 (MQ=60)
                                                                                     TGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCT          <  SRR4302537.2922744/94‑1 (MQ=60)
                                                                                     TGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCT          >  SRR4302537.5233164/1‑94 (MQ=60)
                                                                                     TGCCTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCT          >  SRR4302537.2582610/1‑94 (MQ=60)
                                                                                        CTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAT       <  SRR4302537.2357169/94‑1 (MQ=60)
                                                                                        CTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAT       <  SRR4302537.830775/94‑1 (MQ=60)
                                                                                        CTGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAT       >  SRR4302537.2831035/1‑94 (MQ=60)
                                                                                         TGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATT      <  SRR4302537.2590552/94‑1 (MQ=60)
                                                                                         TGGCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATT      >  SRR4302537.4397496/1‑94 (MQ=60)
                                                                                           GCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTT    >  SRR4302537.1282239/1‑94 (MQ=60)
                                                                                           GCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTT    <  SRR4302537.1092509/94‑1 (MQ=60)
                                                                                           GCGGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTT    <  SRR4302537.5982044/94‑1 (MQ=60)
                                                                                             GGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTC  <  SRR4302537.2121792/94‑1 (MQ=60)
                                                                                             GGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTC  >  SRR4302537.4299930/1‑94 (MQ=60)
                                                                                             GGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTC  <  SRR4302537.5323004/94‑1 (MQ=60)
                                                                                             GGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTC  <  SRR4302537.5287327/94‑1 (MQ=60)
                                                                                             GGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTC  <  SRR4302537.95900/94‑1 (MQ=60)
                                                                                             CGCCACTTCAAAAGTATGCATTTTTAATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTC  <  SRR4302537.631706/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTC  >  NC_000913/2475479‑2475665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑