Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,583,000 A→G S182S (AGT→AGC)  yffG ← putative oxidoreductase Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,583,0000AG99.6% 832.2 / NA 235S182S (AGT→AGC) yffGputative oxidoreductase Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (131/103);  minor base T (1/0);  total (132/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTACTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCAT  >  NC_000913/2582910‑2583089
                                                                                          |                                                                                         
aTAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGTCTGCATTTGTTCGACTTTGCt                                                                                         >  1:3354610/1‑93 (MQ=255)
 tAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTc                                                                                        >  2:2362521/1‑93 (MQ=255)
 tAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTc                                                                                        >  1:3509251/1‑93 (MQ=255)
 tAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTc                                                                                        <  1:1001088/93‑1 (MQ=255)
   aaTTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCAt                                                                                      >  2:2273353/1‑93 (MQ=255)
   aaTTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCAt                                                                                      <  1:2536015/93‑1 (MQ=255)
    aTTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCAtt                                                                                     >  1:5237474/1‑93 (MQ=255)
    aTTTAATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCAtt                                                                                     <  2:1313411/93‑1 (MQ=255)
     tttCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTa                                                                                    >  1:1902680/1‑93 (MQ=255)
      ttCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTAc                                                                                   <  1:1272419/93‑1 (MQ=255)
      ttCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTAc                                                                                   <  1:3894364/93‑1 (MQ=255)
      ttCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTAc                                                                                   <  1:3631709/93‑1 (MQ=255)
       tcatcaAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTAcc                                                                                  <  2:1681615/93‑1 (MQ=255)
       tcatcaAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTAcc                                                                                  <  1:503946/93‑1 (MQ=255)
       tcatcaAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTAcc                                                                                  >  2:360249/1‑93 (MQ=255)
       tcatcaAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTAcc                                                                                  <  2:5318038/93‑1 (MQ=255)
        catcaAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCg                                                                                 <  1:609743/93‑1 (MQ=255)
        catcaAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCg                                                                                 >  2:4334391/1‑93 (MQ=255)
         atcaAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCgg                                                                                >  2:243711/1‑93 (MQ=255)
               aCCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTc                                                                          <  2:5363301/93‑1 (MQ=255)
               aCCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTc                                                                          <  2:2048634/93‑1 (MQ=255)
                ccGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCt                                                                         <  1:480419/93‑1 (MQ=255)
                ccGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCt                                                                         <  1:2493164/93‑1 (MQ=255)
                 cGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCtt                                                                        <  1:3195075/93‑1 (MQ=255)
                 cGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCtt                                                                        >  1:596056/1‑93 (MQ=255)
                 cGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCtt                                                                        >  2:6341342/1‑93 (MQ=255)
                  ggTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTg                                                                       >  1:6008504/1‑93 (MQ=255)
                   gTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTgc                                                                      <  1:5657304/93‑1 (MQ=255)
                        gcgcgcTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGcgccgc                                                                 <  2:4224029/93‑1 (MQ=255)
                        gcgcgcTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGcgccgc                                                                 <  1:4747859/93‑1 (MQ=255)
                        gcgcgcTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTACATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGcgccgc                                                                 <  1:4553328/93‑1 (MQ=255)
                         cgcgcTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCcgcg                                                                >  2:134312/1‑93 (MQ=255)
                          gcgcTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCgg                                                               >  2:3093052/1‑93 (MQ=255)
                            gcTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGCCTTTGCTCATTACCGGCATTTCTTGCGCCGCGGtc                                                             >  2:1095720/1‑92 (MQ=255)
                            gcTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTa                                                             <  2:3037056/93‑1 (MQ=255)
                             cTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTAc                                                            <  1:5774610/93‑1 (MQ=255)
                             cTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTAc                                                            <  2:2891952/93‑1 (MQ=255)
                              ttCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACt                                                           >  1:4886130/1‑93 (MQ=255)
                              ttCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACt                                                           >  1:5491573/1‑93 (MQ=255)
                              ttCAATCGCCAGTTTATCCGGATCGCCACGAGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACt                                                           >  2:2676471/1‑93 (MQ=255)
                                 aaTCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGc                                                        >  2:891764/1‑93 (MQ=255)
                                  aTCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCa                                                       <  1:4370834/93‑1 (MQ=255)
                                   tCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCAt                                                      >  1:3124471/1‑93 (MQ=255)
                                     gCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGc                                                    >  2:5254554/1‑93 (MQ=255)
                                     gCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGc                                                    >  2:2336418/1‑93 (MQ=255)
                                      ccAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGcc                                                   >  1:3986504/1‑93 (MQ=255)
                                       cAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCGGCGGTACTGGCATGCCa                                                  <  2:2463660/93‑1 (MQ=255)
                                       cAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCa                                                  >  2:4824533/1‑93 (MQ=255)
                                       cAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCa                                                  >  2:2704929/1‑93 (MQ=255)
                                        aGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCAc                                                 >  2:3928315/1‑93 (MQ=255)
                                        aGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCAc                                                 <  1:3848330/93‑1 (MQ=255)
                                         gTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACg                                                >  2:6145579/1‑93 (MQ=255)
                                         gTTTATCCGCCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACg                                                >  2:6018685/1‑93 (MQ=255)
                                          tttATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACgg                                               <  1:2662354/93‑1 (MQ=255)
                                           ttATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGt                                              <  2:3366182/93‑1 (MQ=255)
                                           ttATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGt                                              <  1:1441921/93‑1 (MQ=255)
                                            tATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGtt                                             <  2:3624146/93‑1 (MQ=255)
                                            tATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGtt                                             >  1:6423337/1‑93 (MQ=255)
                                              tCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGa                                           <  2:1221766/93‑1 (MQ=255)
                                                cGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGGTGATg                                         >  2:4145906/1‑93 (MQ=255)
                                                   cTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTc                                      <  1:4466506/93‑1 (MQ=255)
                                                    tCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTcc                                     >  1:4194588/1‑93 (MQ=255)
                                                      gCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTg                                   >  2:3682578/1‑93 (MQ=255)
                                                       ccACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGa                                  <  2:6423338/93‑1 (MQ=255)
                                                       ccACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGa                                  >  1:158777/1‑93 (MQ=255)
                                                        cACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGAc                                 <  2:4084545/93‑1 (MQ=255)
                                                        cACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGAc                                 <  1:5574938/93‑1 (MQ=255)
                                                          cgcgcgGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGAcgc                               <  1:4112586/93‑1 (MQ=255)
                                                           gcgcgGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGAcgcg                              >  1:4688021/1‑93 (MQ=255)
                                                              cgGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCgg                           <  2:815203/93‑1 (MQ=255)
                                                               gggCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCggg                          >  1:849850/1‑92 (MQ=255)
                                                               gggCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGt                          <  2:1582731/93‑1 (MQ=255)
                                                               gggCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGt                          <  1:2412284/93‑1 (MQ=255)
                                                                gtcGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTg                         >  2:1136395/3‑93 (MQ=255)
                                                                 gCGTTGCCTGCATTTGTTTGACTTTTCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTgc                        >  2:5015112/1‑93 (MQ=255)
                                                                 gCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTgc                        <  1:4308650/93‑1 (MQ=255)
                                                                   gTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTgcgc                      >  1:2914412/1‑93 (MQ=255)
                                                                     tGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCaa                    >  1:5300107/1‑93 (MQ=255)
                                                                      gtcTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAg                   <  2:3272346/91‑1 (MQ=255)
                                                                      gCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAg                   <  2:3139726/93‑1 (MQ=255)
                                                                      gCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAg                   <  1:5626712/93‑1 (MQ=255)
                                                                      gCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAg                   <  1:3024827/93‑1 (MQ=255)
                                                                      gCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAg                   >  2:4566428/1‑93 (MQ=255)
                                                                       ccTTCATTTGTACGTCTTTGCACATTACCGGCATTTCGTGCGCCGCGTTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAgc                  <  2:5714384/93‑1 (MQ=255)
                                                                       ccTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAgc                  >  2:5082795/1‑93 (MQ=255)
                                                                       ccTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAgc                  <  2:1840044/93‑1 (MQ=255)
                                                                       ccTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAgc                  >  1:277600/1‑93 (MQ=255)
                                                                       ccTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAgc                  <  2:4467428/93‑1 (MQ=255)
                                                                        cTGCATTTGTTCGACTTTGCTCATTCCCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAgcg                 <  2:5481293/93‑1 (MQ=255)
                                                                         tGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAgcgg                >  1:1516358/1‑93 (MQ=255)
                                                                          gCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAgcggc               >  1:2083125/1‑93 (MQ=255)
                                                                            aTTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGcggcgg             <  2:2480231/93‑1 (MQ=255)
                                                                            aTTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGCTGATGTTCCTGACGCGCGGTGCGCAAGcggcgg             >  1:1232855/1‑93 (MQ=255)
                                                                             tttGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCggg            <  1:5594141/93‑1 (MQ=255)
                                                                             tttGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCggg            >  1:2122356/1‑93 (MQ=255)
                                                                             tttGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCggg            >  1:1987826/1‑93 (MQ=255)
                                                                                gTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGAtt         >  1:5982620/1‑93 (MQ=255)
                                                                                 ttCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGAttt        >  1:584360/1‑93 (MQ=255)
                                                                                       tttGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCAt  >  1:4256290/1‑93 (MQ=255)
                                                                                       tttGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCAt  <  2:2620185/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTACTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCAT  >  NC_000913/2582910‑2583089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTACTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCATGCC  >  NC_000913/2582907‑2583092
                                                                                             |                                                                                            
CAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTG                                                                                              <  SRR4302537.6004149/94‑1 (MQ=60)
 AGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGC                                                                                             >  SRR4302537.1940763/1‑94 (MQ=60)
 AGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGC                                                                                             >  SRR4302537.758294/1‑94 (MQ=60)
 AGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGC                                                                                             <  SRR4302537.528244/94‑1 (MQ=60)
 AGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGC                                                                                             >  SRR4302537.185814/1‑94 (MQ=60)
  GATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGTCTGCATTTGTTCGACTTTGCT                                                                                            >  SRR4302537.3378369/1‑94 (MQ=60)
  GATAAATTTCATCAAAACCGGGTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTCTTCGACTTTGCT                                                                                            >  SRR4302537.2092638/1‑94 (MQ=60)
   ATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTC                                                                                           >  SRR4302537.389322/1‑94 (MQ=60)
   ATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTC                                                                                           >  SRR4302537.3534080/1‑94 (MQ=60)
    TAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCA                                                                                          <  SRR4302537.1007999/94‑1 (MQ=60)
    TAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCA                                                                                          >  SRR4302537.2725513/1‑94 (MQ=60)
     AAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCAT                                                                                         <  SRR4302537.1590677/94‑1 (MQ=60)
     AAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCAT                                                                                         <  SRR4302537.5611249/94‑1 (MQ=60)
     AAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCAT                                                                                         >  SRR4302537.147407/1‑94 (MQ=60)
      AATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATT                                                                                        <  SRR4302537.2553810/94‑1 (MQ=60)
      AATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATT                                                                                        >  SRR4302537.5273511/1‑94 (MQ=60)
      AATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATT                                                                                        <  SRR4302537.4457771/94‑1 (MQ=60)
      AATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATT                                                                                        <  SRR4302537.990465/94‑1 (MQ=60)
       ATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTA                                                                                       >  SRR4302537.1915895/1‑94 (MQ=60)
         TTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACC                                                                                     <  SRR4302537.3921588/94‑1 (MQ=60)
         TTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACC                                                                                     <  SRR4302537.3657298/94‑1 (MQ=60)
         TTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACC                                                                                     <  SRR4302537.1281264/94‑1 (MQ=60)
          TCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCG                                                                                    <  SRR4302537.507411/94‑1 (MQ=60)
           CATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGG                                                                                   >  SRR4302537.4882445/1‑94 (MQ=60)
           CATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGG                                                                                   <  SRR4302537.613910/94‑1 (MQ=60)
            ATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGC                                                                                  >  SRR4302537.1430378/1‑94 (MQ=60)
             TCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCA                                                                                 >  SRR4302537.5688864/1‑94 (MQ=60)
             TCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCA                                                                                 >  SRR4302537.5867199/1‑94 (MQ=60)
              CAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCAT                                                                                <  SRR4302537.5662617/94‑1 (MQ=60)
              CAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCAT                                                                                <  SRR4302537.2582271/94‑1 (MQ=60)
               AAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATT                                                                               <  SRR4302537.378310/94‑1 (MQ=60)
                  ACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCT                                                                            <  SRR4302537.6022989/94‑1 (MQ=60)
                  ACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCT                                                                            >  SRR4302537.4247925/1‑94 (MQ=60)
                  ACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCT                                                                            <  SRR4302537.284403/94‑1 (MQ=60)
                   CCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTT                                                                           <  SRR4302537.2510720/94‑1 (MQ=60)
                   CCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTT                                                                           >  SRR4302537.4323592/1‑94 (MQ=60)
                   CCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTT                                                                           <  SRR4302537.483712/94‑1 (MQ=60)
                   CCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTT                                                                           >  SRR4302537.600131/1‑94 (MQ=60)
                    CGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTG                                                                          <  SRR4302537.3217540/94‑1 (MQ=60)
                    CGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTG                                                                          >  SRR4302537.6049789/1‑94 (MQ=60)
                     GGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGCTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGC                                                                         >  SRR4302537.545310/1‑94 (MQ=60)
                      GTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCG                                                                        <  SRR4302537.5696021/94‑1 (MQ=60)
                           GCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCG                                                                   <  SRR4302537.4780659/94‑1 (MQ=60)
                           GCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTACATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCG                                                                   <  SRR4302537.4584835/94‑1 (MQ=60)
                              CGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTA                                                                <  SRR4302537.4111850/94‑1 (MQ=60)
                               GCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTAC                                                               >  SRR4302537.2145003/1‑94 (MQ=60)
                               GCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTAC                                                               >  SRR4302537.1269357/1‑94 (MQ=60)
                                CTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACT                                                              >  SRR4302537.4155513/1‑94 (MQ=60)
                                CTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACT                                                              >  SRR4302537.4919871/1‑94 (MQ=60)
                                CTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACT                                                              <  SRR4302537.5814190/94‑1 (MQ=60)
                                CTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACT                                                              >  SRR4302537.5529062/1‑94 (MQ=60)
                                  TCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGG                                                            >  SRR4302537.5649727/1‑94 (MQ=60)
                                   CAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGTATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGC                                                           >  SRR4302537.4588904/1‑94 (MQ=60)
                                   CAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGC                                                           >  SRR4302537.3209681/1‑94 (MQ=60)
                                     ATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCAT                                                         <  SRR4302537.4401036/94‑1 (MQ=60)
                                     ATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCAT                                                         >  SRR4302537.3146348/1‑94 (MQ=60)
                                      TCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATG                                                        <  SRR4302537.3456732/94‑1 (MQ=60)
                                        GCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCC                                                      <  SRR4302537.5987214/94‑1 (MQ=60)
                                        GCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCC                                                      >  SRR4302537.4014419/1‑94 (MQ=60)
                                          CAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCAC                                                    >  SRR4302537.1992338/1‑94 (MQ=60)
                                          CAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCAC                                                    >  SRR4302537.4862718/1‑94 (MQ=60)
                                          CAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCAC                                                    >  SRR4302537.5578834/1‑94 (MQ=60)
                                           AGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACG                                                   <  SRR4302537.3875278/94‑1 (MQ=60)
                                             TTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGT                                                 <  SRR4302537.2680954/94‑1 (MQ=60)
                                              TTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTT                                                <  SRR4302537.1451970/94‑1 (MQ=60)
                                              TTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTT                                                >  SRR4302537.6047411/1‑94 (MQ=60)
                                              TTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTT                                                <  SRR4302537.4695522/94‑1 (MQ=60)
                                              TTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTT                                                >  SRR4302537.6466991/1‑94 (MQ=60)
                                                  CCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATG                                            >  SRR4302537.5770510/1‑94 (MQ=60)
                                                   CGGCTCGCCACGCGCGGGCGTTGCCTGCGTTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGT                                           <  SRR4302537.4514412/94‑1 (MQ=60)
                                                   CGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGT                                           <  SRR4302537.3211283/94‑1 (MQ=60)
                                                   CGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGT                                           <  SRR4302537.3720249/94‑1 (MQ=60)
                                                    GGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTT                                          <  SRR4302537.5750794/94‑1 (MQ=60)
                                                      CTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCC                                        <  SRR4302537.4497397/94‑1 (MQ=60)
                                                      CTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCC                                        >  SRR4302537.4223884/1‑94 (MQ=60)
                                                      CTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCC                                        <  SRR4302537.284743/94‑1 (MQ=60)
                                                      CTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCC                                        >  SRR4302537.4098193/1‑94 (MQ=60)
                                                        CGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTG                                      >  SRR4302537.2545284/1‑94 (MQ=60)
                                                         GCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGA                                     >  SRR4302537.159905/1‑94 (MQ=60)
                                                          CCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGAC                                    <  SRR4302537.2112263/94‑1 (MQ=60)
                                                          CCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGAC                                    <  SRR4302537.679238/94‑1 (MQ=60)
                                                           CACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACG                                   <  SRR4302537.5613042/94‑1 (MQ=60)
                                                           CACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACG                                   <  SRR4302537.5124265/94‑1 (MQ=60)
                                                             CGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCG                                 >  SRR4302537.4720453/1‑94 (MQ=60)
                                                             CGCGCGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCG                                 <  SRR4302537.4141314/94‑1 (MQ=60)
                                                                 CGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGT                             <  SRR4302537.5026103/94‑1 (MQ=60)
                                                                 CGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGT                             >  SRR4302537.5288002/1‑94 (MQ=60)
                                                                 CGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGG                             >  SRR4302537.855678/1‑94 (MQ=60)
                                                                 CGGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGG                             >  SRR4302537.3508230/1‑94 (MQ=60)
                                                                 CGGGCGTTGCATGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGT                             <  SRR4302537.1529358/94‑1 (MQ=60)
                                                                  GGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTG                            <  SRR4302537.2429267/94‑1 (MQ=60)
                                                                  GGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTG                            >  SRR4302537.4766540/1‑94 (MQ=60)
                                                                  GGGCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTG                            >  SRR4302537.6249784/1‑94 (MQ=60)
                                                                    GCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCG                          <  SRR4302537.3033546/94‑1 (MQ=60)
                                                                    GCGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCG                          <  SRR4302537.4338597/94‑1 (MQ=60)
                                                                     CGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGC                         >  SRR4302537.4391913/1‑94 (MQ=60)
                                                                     CGTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGC                         >  SRR4302537.2934882/1‑94 (MQ=60)
                                                                      GTTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCA                        >  SRR4302537.910273/1‑94 (MQ=60)
                                                                       TTGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAA                       >  SRR4302537.5336511/1‑94 (MQ=60)
                                                                        TGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAG                      <  SRR4302537.4804838/94‑1 (MQ=60)
                                                                        TGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAG                      <  SRR4302537.6122383/94‑1 (MQ=60)
                                                                        TGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAG                      >  SRR4302537.2232143/1‑94 (MQ=60)
                                                                        TGCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAG                      >  SRR4302537.3545521/1‑94 (MQ=60)
                                                                         GCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGC                     >  SRR4302537.279541/1‑94 (MQ=60)
                                                                         GCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGC                     <  SRR4302537.3045916/94‑1 (MQ=60)
                                                                         GCCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGC                     <  SRR4302537.5665206/94‑1 (MQ=60)
                                                                          CCTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCG                    >  SRR4302537.2029382/1‑94 (MQ=60)
                                                                           CTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGG                   >  SRR4302537.1526954/1‑94 (MQ=60)
                                                                           CTGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGG                   >  SRR4302537.5217603/1‑94 (MQ=60)
                                                                            TGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGC                  <  SRR4302537.173377/94‑1 (MQ=60)
                                                                            TGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGC                  >  SRR4302537.6020610/1‑94 (MQ=60)
                                                                            TGCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGC                  >  SRR4302537.2097797/1‑94 (MQ=60)
                                                                             GCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCG                 >  SRR4302537.3278962/1‑94 (MQ=60)
                                                                             GCATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCG                 <  SRR4302537.30991/94‑1 (MQ=60)
                                                                              CATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGG                >  SRR4302537.2478342/1‑94 (MQ=60)
                                                                              CATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGCTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGG                >  SRR4302537.1241431/1‑94 (MQ=60)
                                                                               ATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGG               >  SRR4302537.2137289/1‑94 (MQ=60)
                                                                               ATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGG               >  SRR4302537.2001647/1‑94 (MQ=60)
                                                                               ATTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGG               <  SRR4302537.6130351/94‑1 (MQ=60)
                                                                                TTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGG              >  SRR4302537.5621800/1‑94 (MQ=60)
                                                                                TTTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGA              <  SRR4302537.5632390/94‑1 (MQ=60)
                                                                                 TTGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGAT             <  SRR4302537.4421502/94‑1 (MQ=60)
                                                                                  TGTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATT            >  SRR4302537.6023685/1‑94 (MQ=60)
                                                                                   GTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTT           >  SRR4302537.588350/1‑94 (MQ=60)
                                                                                   GTTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTT           <  SRR4302537.3996681/94‑1 (MQ=60)
                                                                                    TTCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGGGGCGGGGTTTC          >  SRR4302537.1155193/1‑94 (MQ=60)
                                                                                     TCGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCG         <  SRR4302537.1281916/94‑1 (MQ=60)
                                                                                      CGACTTTGCTCATTACCGGCATTTCTTGTGCCGCGGTACTGGCATGCCACGGCTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGGTTTCGC        >  SRR4302537.2575230/1‑94 (MQ=60)
                                                                                      CGACTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGC        >  SRR4302537.4226727/1‑94 (MQ=60)
                                                                                         CTTTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCAT     >  SRR4302537.4285871/1‑94 (MQ=60)
                                                                                           TTGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCATGC   <  SRR4302537.3850511/94‑1 (MQ=60)
                                                                                            TGCTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCATGCC  <  SRR4302537.4217219/94‑1 (MQ=60)
                                                                                             |                                                                                            
CAGATAAATTTCATCAAAACCGGTTTTGCGCGCTTCAATCGCCAGTTTATCCGGCTCGCCACGCGCGGGCGTTGCCTGCATTTGTTCGACTTTACTCATTACCGGCATTTCTTGCGCCGCGGTACTGGCATGCCACGGTTGATGTTCCTGACGCGCGGTGCGCAAGCGGCGGGATTTCGCCATGCC  >  NC_000913/2582907‑2583092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑