Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,320,557	T→C	P385P (CCA→CCG)	mrsA ←	MrsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,320,557	0	T	C	99.6%	870.8 / ‑5.0	248	P385P (CCA→CCG)	mrsA	MrsA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (99/148); minor base A (0/1); total (99/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

Only 100 of 253 total aligned reads displayed.

GGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATG  >  NC_000913/3320467‑3320646

ggTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCgg                                                                                         <  1:6388291/93‑1 (MQ=255)

  tGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGAt                                                                                       <  2:386915/93‑1 (MQ=255)

   gCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATc                                                                                      <  2:86291/93‑1 (MQ=255)

    ccGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATcg                                                                                     >  1:3207151/1‑93 (MQ=255)

     cGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATcgc                                                                                    <  1:1938441/93‑1 (MQ=255)

     cGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATcgc                                                                                    <  1:1044585/93‑1 (MQ=255)

     cGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATcgc                                                                                    <  2:1541012/93‑1 (MQ=255)

     cGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATcgc                                                                                    <  2:1651843/93‑1 (MQ=255)

      ggATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATcgcc                                                                                   >  2:2037183/1‑93 (MQ=255)

       gATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATcgccg                                                                                  >  1:3895780/1‑93 (MQ=255)

       gATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATcgccg                                                                                  >  2:2646795/1‑93 (MQ=255)

       gATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCAGATcgccg                                                                                  >  2:5333814/1‑93 (MQ=255)

         tttACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCt                                                                                >  1:865906/1‑93 (MQ=255)

         tttACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCt                                                                                >  2:4205732/1‑93 (MQ=255)

          ttACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTa                                                                               <  1:5739467/93‑1 (MQ=255)

           tACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTCACTGACTCATGCTCAAGCGGATCGCCGCTAc                                                                              <  2:5167913/93‑1 (MQ=255)

            aCGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTAcc                                                                             <  2:2480459/93‑1 (MQ=255)

             cGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCt                                                                            >  2:1869221/1‑93 (MQ=255)

               cagcaACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGc                                                                          <  1:4431620/93‑1 (MQ=255)

                agcaACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACTGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCg                                                                         <  1:649973/93‑1 (MQ=255)

                 gcaACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCgg                                                                        >  2:2266948/1‑93 (MQ=255)

                 gcaACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCgg                                                                        >  2:3130542/1‑93 (MQ=255)

                  caACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGgt                                                                       <  1:5801962/93‑1 (MQ=255)

                    acacGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGgtgt                                                                     <  1:2772245/93‑1 (MQ=255)

                    acacGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGgtgt                                                                     >  2:3562316/1‑93 (MQ=255)

                     cacGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTa                                                                    <  1:705219/93‑1 (MQ=255)

                      acGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTaa                                                                   <  1:2874372/93‑1 (MQ=255)

                      acGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTaa                                                                   >  2:2886646/1‑93 (MQ=255)

                       cgcgTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAac                                                                  >  2:435256/1‑93 (MQ=255)

                       cgcgTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAac                                                                  <  2:1338496/93‑1 (MQ=255)

                       cgcgTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAac                                                                  <  1:1091989/93‑1 (MQ=255)

                           tCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAacgtac                                                              >  2:1797884/1‑93 (MQ=255)

                              aCGGTTGCCCAGGGCAGCTTCAACCTCTGCGGTCACGGCTCTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGCGTAACGTACGtt                                                           >  2:747675/1‑93 (MQ=255)

                              aCGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGtt                                                           >  2:6353173/1‑93 (MQ=255)

                              aCGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGtt                                                           <  2:6239823/93‑1 (MQ=255)

                              aCGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGtt                                                           <  2:4990815/93‑1 (MQ=255)

                              aCGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGtt                                                           >  1:2637107/1‑93 (MQ=255)

                               cGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTa                                                          <  1:1431920/93‑1 (MQ=255)

                               cGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTa                                                          <  1:4365333/93‑1 (MQ=255)

                                  ttGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAAcc                                                       <  1:5971012/93‑1 (MQ=255)

                                   tGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCa                                                      <  1:1819467/93‑1 (MQ=255)

                                     cccAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTCAACCAGa                                                    <  2:5079903/93‑1 (MQ=255)

                                      ccAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGaa                                                   <  2:5195499/93‑1 (MQ=255)

                                      ccAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGaa                                                   <  2:4754484/93‑1 (MQ=255)

                                      ccAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGaa                                                   <  2:3388069/93‑1 (MQ=255)

                                       cAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAAt                                                  <  2:5622633/93‑1 (MQ=255)

                                         gcgcAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCt                                                <  1:4883690/93‑1 (MQ=255)

                                           gcAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGc                                              >  1:5496183/1‑93 (MQ=255)

                                           gcAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGc                                              <  1:3662561/93‑1 (MQ=255)

                                           gcAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGc                                              >  2:3252465/1‑93 (MQ=255)

                                            cAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCg                                             <  2:4569280/93‑1 (MQ=255)

                                            cAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCg                                             <  1:4166007/93‑1 (MQ=255)

                                            cAGCTGCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCg                                             <  1:4620741/93‑1 (MQ=255)

                                             aGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCgg                                            <  1:3535475/93‑1 (MQ=255)

                                             aGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCgg                                            <  1:2158272/93‑1 (MQ=255)

                                              gCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGgggg                                           >  1:1571578/1‑93 (MQ=255)

                                              gCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCggg                                           <  1:5663918/93‑1 (MQ=255)

                                               cTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGa                                          >  1:4812304/1‑93 (MQ=255)

                                               cTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGa                                          <  1:1855319/93‑1 (MQ=255)

                                                 tCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAAc                                        >  1:1229077/1‑93 (MQ=255)

                                                 tCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAAc                                        >  1:3619660/1‑93 (MQ=255)

                                                  cAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACa                                       >  2:2163811/1‑93 (MQ=255)

                                                   aaCCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACAt                                      >  2:2683992/1‑93 (MQ=255)

                                                     ccTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACAttt                                    <  2:4813766/93‑1 (MQ=255)

                                                     ccTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACAttt                                    >  2:4121252/1‑93 (MQ=255)

                                                     ccTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACAttt                                    >  2:3168565/1‑93 (MQ=255)

                                                      ctctGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACAtttt                                   <  1:751867/93‑1 (MQ=255)

                                                        ctGCGTTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCa                                 <  2:255278/93‑1 (MQ=255)

                                                        ctGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCa                                 <  2:6423196/93‑1 (MQ=255)

                                                        ctGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCa                                 <  2:3419170/93‑1 (MQ=255)

                                                        ctGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCa                                 <  2:1461253/93‑1 (MQ=255)

                                                            ggTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGcc                             <  2:3584789/93‑1 (MQ=255)

                                                            ggTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGcc                             <  1:2996192/93‑1 (MQ=255)

                                                            ggTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGcc                             >  2:1111559/1‑93 (MQ=255)

                                                             gTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCg                            >  2:5921682/1‑93 (MQ=255)

                                                              tCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGc                           <  2:5551465/93‑1 (MQ=255)

                                                              tCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGc                           <  1:618125/93‑1 (MQ=255)

                                                              tCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGc                           <  1:4696976/93‑1 (MQ=255)

                                                               cACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCt                          <  1:2054372/93‑1 (MQ=255)

                                                               cACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCt                          <  1:5091763/93‑1 (MQ=255)

                                                                aCGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTg                         >  2:6172149/1‑93 (MQ=255)

                                                                aCGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTg                         >  2:66511/1‑93 (MQ=255)

                                                                aCGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTACGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTg                         >  1:4555305/1‑93 (MQ=255)

                                                                   gCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCaa                      >  1:916938/1‑93 (MQ=255)

                                                                    cTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTACCCAGAATCTGCGGGAACATTTTCATGCCGCTGCaaa                     <  2:3812408/93‑1 (MQ=255)

                                                                    cTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCaaa                     <  2:5681759/93‑1 (MQ=255)

                                                                    cTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCaaa                     <  1:4302419/93‑1 (MQ=255)

                                                                      ttAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAgg                   <  2:2792655/93‑1 (MQ=255)

                                                                        aaCTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTc                 <  1:6450705/93‑1 (MQ=255)

                                                                        aaCTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTc                 <  1:357603/93‑1 (MQ=255)

                                                                           tGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTg              >  2:4057795/1‑93 (MQ=255)

                                                                           tGACTCATGCGCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTg              <  2:3982136/93‑1 (MQ=255)

                                                                              cTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAg           <  2:5454461/93‑1 (MQ=255)

                                                                              cTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAg           <  1:2281945/93‑1 (MQ=255)

                                                                               tCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAgg          >  2:1028746/1‑93 (MQ=255)

                                                                                cATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGc         <  1:5347183/93‑1 (MQ=255)

                                                                                cATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGc         <  1:495941/93‑1 (MQ=255)

                                                                                   gCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCa      >  2:4957819/1‑93 (MQ=255)

                                                                                     tCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCata    <  1:3810226/93‑1 (MQ=255)

                                                                                       aaGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATg  >  1:1659640/1‑93 (MQ=255)

GGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATG  >  NC_000913/3320467‑3320646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGGTT  >  NC_000913/3320464‑3320649

TTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGC                                                                                              <  SRR4302537.5032721/94‑1 (MQ=60)

TTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGC                                                                                              <  SRR4302537.5233047/94‑1 (MQ=60)

 TCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCG                                                                                             <  SRR4302537.4174649/94‑1 (MQ=60)

  CGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGG                                                                                            <  SRR4302537.3770130/94‑1 (MQ=60)

  CGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGG                                                                                            <  SRR4302537.5480087/94‑1 (MQ=60)

   GGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGA                                                                                           <  SRR4302537.6431822/94‑1 (MQ=60)

     TGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATC                                                                                         <  SRR4302537.3674761/94‑1 (MQ=60)

     TGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATC                                                                                         <  SRR4302537.2393300/94‑1 (MQ=60)

     TGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATC                                                                                         <  SRR4302537.852655/94‑1 (MQ=60)

      GCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCG                                                                                        >  SRR4302537.3229698/1‑94 (MQ=60)

      GCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCG                                                                                        <  SRR4302537.444919/94‑1 (MQ=60)

        CGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCC                                                                                      <  SRR4302537.6509450/94‑1 (MQ=60)

        CGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCC                                                                                      >  SRR4302537.2980439/1‑94 (MQ=60)

        CGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCC                                                                                      <  SRR4302537.4824474/94‑1 (MQ=60)

        CGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCC                                                                                      <  SRR4302537.1051792/94‑1 (MQ=60)

        CGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCC                                                                                      <  SRR4302537.1951919/94‑1 (MQ=60)

        CGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCC                                                                                      <  SRR4302537.6443240/94‑1 (MQ=60)

        CGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCC                                                                                      >  SRR4302537.3756578/1‑94 (MQ=60)

         GGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCG                                                                                     >  SRR4302537.3923015/1‑94 (MQ=60)

         GGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCC                                                                                     >  SRR4302537.1095610/1‑94 (MQ=60)

          GATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGC                                                                                    >  SRR4302537.4646001/1‑94 (MQ=60)

          GATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGC                                                                                    >  SRR4302537.5113586/1‑94 (MQ=60)

           ATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCT                                                                                   >  SRR4302537.871846/1‑94 (MQ=60)

            TTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTA                                                                                  >  SRR4302537.275643/1‑94 (MQ=60)

             TTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTAC                                                                                 <  SRR4302537.5778766/94‑1 (MQ=60)

             TTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTAC                                                                                 >  SRR4302537.3607823/1‑94 (MQ=60)

               ACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCT                                                                               <  SRR4302537.4305336/94‑1 (MQ=60)

                  CAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCG                                                                            <  SRR4302537.4462224/94‑1 (MQ=60)

                   AGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACTGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGG                                                                           <  SRR4302537.654401/94‑1 (MQ=60)

                   AGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGG                                                                           >  SRR4302537.2217829/1‑94 (MQ=60)

                   AGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGG                                                                           <  SRR4302537.4176226/94‑1 (MQ=60)

                    GCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGT                                                                          <  SRR4302537.2143204/94‑1 (MQ=60)

                     CAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTG                                                                         >  SRR4302537.4924140/1‑94 (MQ=60)

                     CAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTG                                                                         <  SRR4302537.5841742/94‑1 (MQ=60)

                       ACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTA                                                                       <  SRR4302537.2791619/94‑1 (MQ=60)

                        CACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAA                                                                      <  SRR4302537.710043/94‑1 (MQ=60)

                         ACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAAC                                                                     >  SRR4302537.4916706/1‑94 (MQ=60)

                         ACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAAC                                                                     <  SRR4302537.2894544/94‑1 (MQ=60)

                          CGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACG                                                                    >  SRR4302537.4890184/1‑94 (MQ=60)

                          CGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACG                                                                    <  SRR4302537.1099529/94‑1 (MQ=60)

                          CGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACG                                                                    <  SRR4302537.1683396/94‑1 (MQ=60)

                          CGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCGTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACG                                                                    <  SRR4302537.598858/94‑1 (MQ=60)

                            CGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTA                                                                  >  SRR4302537.5832402/1‑94 (MQ=60)

                             GTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTAC                                                                 >  SRR4302537.1138202/1‑94 (MQ=60)

                              TCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACG                                                                >  SRR4302537.581102/1‑94 (MQ=60)

                              TCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTAAG                                                                <  SRR4302537.2077814/94‑1 (MQ=60)

                               cggactgtgctcttgtctctaccttttgggcggtaggggttTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGT                                                               <  SRR4302537.5148003/53‑1 (MQ=60)

                                CACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTT                                                              >  SRR4302537.2655554/1‑94 (MQ=60)

                                 ACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATAGCCGCTACCTGCGGTGTAACGTACGTTA                                                             >  SRR4302537.2869240/1‑94 (MQ=60)

                                 ACGGTTGCCCAGCGCAGCTTCAACCGCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTTCCTGCGGTGTAACGTACGTTA                                                             <  SRR4302537.999830/94‑1 (MQ=60)

                                  CGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAA                                                            >  SRR4302537.2803679/1‑94 (MQ=60)

                                  CGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAA                                                            <  SRR4302537.1441886/94‑1 (MQ=60)

                                  CGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAA                                                            <  SRR4302537.4395518/94‑1 (MQ=60)

                                   GGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAAC                                                           <  SRR4302537.6122863/94‑1 (MQ=60)

                                   GGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAAC                                                           >  SRR4302537.3561017/1‑94 (MQ=60)

                                   GGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAAA                                                           >  SRR4302537.2430889/1‑94 (MQ=60)

                                   GGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGAAACGTACGTTAAC                                                           >  SRR4302537.142969/1‑94 (MQ=60)

                                     TTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCA                                                         <  SRR4302537.6011997/94‑1 (MQ=60)

                                      TGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAG                                                        <  SRR4302537.1832147/94‑1 (MQ=60)

                                      TGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAG                                                        <  SRR4302537.1519830/94‑1 (MQ=60)

                                        CCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAA                                                      <  SRR4302537.6463640/94‑1 (MQ=60)

                                        CCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAA                                                      <  SRR4302537.888878/94‑1 (MQ=60)

                                           AGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCT                                                   <  SRR4302537.4582201/94‑1 (MQ=60)

                                           AGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCT                                                   >  SRR4302537.1643715/1‑94 (MQ=60)

                                            GCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTG                                                  <  SRR4302537.4917419/94‑1 (MQ=60)

                                            GCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTG                                                  <  SRR4302537.5767685/94‑1 (MQ=60)

                                             CGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTACCCAGAGTCTGC                                                 >  SRR4302537.673120/1‑94 (MQ=60)

                                             CGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGC                                                 >  SRR4302537.4360530/1‑94 (MQ=60)

                                             CGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGC                                                 >  SRR4302537.5533701/1‑94 (MQ=60)

                                              GCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCG                                                <  SRR4302537.3688338/94‑1 (MQ=60)

                                              GCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCG                                                <  SRR4302537.5167639/94‑1 (MQ=60)

                                               CAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGG                                               <  SRR4302537.4195103/94‑1 (MQ=60)

                                               CAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGG                                               <  SRR4302537.5694074/94‑1 (MQ=60)

                                               CAGCTGCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGG                                               <  SRR4302537.4652734/94‑1 (MQ=60)

                                                AGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGGGGG                                              >  SRR4302537.1582545/1‑94 (MQ=60)

                                                AGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGG                                              <  SRR4302537.2173483/94‑1 (MQ=60)

                                                AGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGG                                              <  SRR4302537.3560452/94‑1 (MQ=60)

                                                 GCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGA                                             <  SRR4302537.5702673/94‑1 (MQ=60)

                                                 GCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGA                                             <  SRR4302537.3056835/94‑1 (MQ=60)

                                                 GCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGA                                             >  SRR4302537.4845526/1‑94 (MQ=60)

                                                  CTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAA                                            >  SRR4302537.6013413/1‑94 (MQ=60)

                                                  CTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAA                                            <  SRR4302537.1868220/94‑1 (MQ=60)

                                                   TTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAAC                                           >  SRR4302537.3645173/1‑94 (MQ=60)

                                                   TTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAAC                                           >  SRR4302537.1237625/1‑94 (MQ=60)

                                                   TTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAAC                                           <  SRR4302537.3884842/94‑1 (MQ=60)

                                                    TCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACA                                          >  SRR4302537.3332186/1‑94 (MQ=60)

                                                      AACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATT                                        <  SRR4302537.5326833/94‑1 (MQ=60)

                                                       ACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTT                                       >  SRR4302537.4657437/1‑94 (MQ=60)

                                                       ACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTT                                       <  SRR4302537.719356/94‑1 (MQ=60)

                                                       ACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTT                                       <  SRR4302537.5665671/94‑1 (MQ=60)

                                                         CTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTC                                     <  SRR4302537.3048943/94‑1 (MQ=60)

                                                         CTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTC                                     <  SRR4302537.756994/94‑1 (MQ=60)

                                                          TCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCA                                    <  SRR4302537.655353/94‑1 (MQ=60)

                                                              CGGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCC                                >  SRR4302537.2066583/1‑94 (MQ=60)

                                                               GGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCG                               <  SRR4302537.3017085/94‑1 (MQ=60)

                                                               GGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCG                               <  SRR4302537.3609327/94‑1 (MQ=60)

                                                               GGTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCG                               >  SRR4302537.93065/1‑94 (MQ=60)

                                                                GTCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGC                              >  SRR4302537.3776/1‑94 (MQ=60)

                                                                 TCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCT                             <  SRR4302537.4729470/94‑1 (MQ=60)

                                                                 TCACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCT                             <  SRR4302537.622331/94‑1 (MQ=60)

                                                                  CACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTG                            <  SRR4302537.769679/94‑1 (MQ=60)

                                                                  CACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTG                            <  SRR4302537.5126822/94‑1 (MQ=60)

                                                                  CACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTG                            <  SRR4302537.2068784/94‑1 (MQ=60)

                                                                  CACGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTACGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTG                            >  SRR4302537.4586845/1‑94 (MQ=60)

                                                                    CGGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCA                          >  SRR4302537.1833126/1‑94 (MQ=60)

                                                                     GGCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAA                         >  SRR4302537.923271/1‑94 (MQ=60)

                                                                      GCTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAA                        >  SRR4302537.4355107/1‑94 (MQ=60)

                                                                       CTTTAACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAG                       <  SRR4302537.4332317/94‑1 (MQ=60)

                                                                           AACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCG                   <  SRR4302537.360084/94‑1 (MQ=60)

                                                                           AACTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCG                   <  SRR4302537.6494448/94‑1 (MQ=60)

                                                                             CTGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTG                 >  SRR4302537.5516748/1‑94 (MQ=60)

                                                                              TGACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGC                <  SRR4302537.5029807/94‑1 (MQ=60)

                                                                               GACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAAACAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCA               >  SRR4302537.3292983/1‑94 (MQ=60)

                                                                                ACTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAG              <  SRR4302537.1898455/94‑1 (MQ=60)

                                                                                 CTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGG             <  SRR4302537.2298048/94‑1 (MQ=60)

                                                                                 CTCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGG             <  SRR4302537.1123410/94‑1 (MQ=60)

                                                                                 CTAATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACAAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGG             <  SRR4302537.4093899/94‑1 (MQ=60)

                                                                                  TCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGC            <  SRR4302537.1882577/94‑1 (MQ=60)

                                                                                  TCATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGC            <  SRR4302537.465911/94‑1 (MQ=60)

                                                                                   CATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCT           <  SRR4302537.5383818/94‑1 (MQ=60)

                                                                                   CATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCT           <  SRR4302537.499354/94‑1 (MQ=60)

                                                                                    ATGCTCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTC          >  SRR4302537.1519147/1‑94 (MQ=60)

                                                                                        TCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATAT      <  SRR4302537.1686690/94‑1 (MQ=60)

                                                                                        TCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATAT      <  SRR4302537.3836939/94‑1 (MQ=60)

                                                                                        TCAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATCTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATAT      <  SRR4302537.6349898/94‑1 (MQ=60)

                                                                                         CAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATG     >  SRR4302537.1671274/1‑94 (MQ=60)

                                                                                         CAAGCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATG     <  SRR4302537.4564609/94‑1 (MQ=60)

                                                                                            GCGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGGTT  <  SRR4302537.5454511/94‑1 (MQ=60)

TTCGGTGCCGGATTTACGCAGCAACACGCGTCCACGGTTGCCCAGCGCAGCTTCAACCTCTGCGGTCACGGCTTTAACTGACTCATGCTCAAGTGGATCGCCGCTACCTGCGGTGTAACGTACGTTAACCAGAATCTGCGGGAACATTTTCATGCCGCTGCAAAGGTCGTGCAGGCTCATATGGTT  >  NC_000913/3320464‑3320649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`