Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,012,261 A→G intergenic (‑1/+47) b2875 ← / ← b2876 putative synthases/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,012,2610AG83.3% 644.8 / 117.9 287intergenic (‑1/+47)b2875/b2876putative synthases/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (20/28);  new base G (123/116);  total (143/144)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 287 total aligned reads displayed.

GGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATA  >  NC_000913/3012171‑3012350
                                                                                          |                                                                                         
  gAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCaa                                                                                       <  1:1644781/93‑1 (MQ=255)
    gTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAAcc                                                                                     <  1:2903015/93‑1 (MQ=255)
      cGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTc                                                                                   <  1:1242717/93‑1 (MQ=255)
        aGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCaa                                                                                 <  1:1002745/93‑1 (MQ=255)
        aGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCaa                                                                                 <  2:240627/93‑1 (MQ=255)
        aGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCaa                                                                                 <  1:6644889/93‑1 (MQ=255)
           cTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATgg                                                                              >  1:4281450/1‑93 (MQ=255)
           cTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATgg                                                                              >  2:7294900/1‑93 (MQ=255)
            tCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGa                                                                             <  1:7199583/93‑1 (MQ=255)
            tCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGa                                                                             <  2:1698636/93‑1 (MQ=255)
            tCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGa                                                                             >  2:219149/1‑93 (MQ=255)
             cGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGat                                                                            >  1:235896/1‑93 (MQ=255)
              ggCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGata                                                                           <  1:4774691/93‑1 (MQ=255)
              ggCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGata                                                                           >  1:1698029/1‑93 (MQ=255)
               gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGatat                                                                          >  1:763643/1‑93 (MQ=255)
                cTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATc                                                                         >  2:3507023/1‑93 (MQ=255)
                 tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATcc                                                                        >  1:4467539/1‑93 (MQ=255)
                 tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATcc                                                                        <  2:3334082/93‑1 (MQ=255)
                 tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATcc                                                                        <  1:6877304/93‑1 (MQ=255)
                  aTCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCt                                                                       >  2:6168648/1‑93 (MQ=255)
                  aTCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTAAAAATATTCATGCCAACCTCAATGGATATCCt                                                                       <  1:6174082/93‑1 (MQ=255)
                   tCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCtt                                                                      >  1:5038788/1‑93 (MQ=255)
                   tCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCtt                                                                      <  1:2055383/93‑1 (MQ=255)
                    caacaaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGACATCCttt                                                                     <  1:42565/93‑1 (MQ=255)
                     aacaaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTc                                                                    <  1:1849694/93‑1 (MQ=255)
                      acaaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCa                                                                   <  1:5856650/93‑1 (MQ=255)
                        aaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGt                                                                 <  1:5121032/93‑1 (MQ=255)
                         aTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTa                                                                >  1:1028504/1‑93 (MQ=255)
                           ttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAAc                                                              <  2:150137/93‑1 (MQ=255)
                            tGCGCCATAGCAAACGGGCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCACCCTCAATGGATATCCTTTCAGTAAcc                                                             <  1:5407028/93‑1 (MQ=255)
                            tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAAcc                                                             <  2:5056451/93‑1 (MQ=255)
                             gcgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAAccc                                                            >  2:6809163/1‑93 (MQ=255)
                                 cATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGaa                                                        <  1:4423718/93‑1 (MQ=255)
                                  aTAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAAt                                                       <  2:890479/93‑1 (MQ=255)
                                  aTAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAAt                                                       <  2:6145941/93‑1 (MQ=255)
                                   tAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATa                                                      >  2:6066336/1‑93 (MQ=255)
                                   tAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATa                                                      >  2:559169/1‑93 (MQ=255)
                                     gCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATAcc                                                    >  1:3390815/1‑93 (MQ=255)
                                     gCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATAcc                                                    <  1:5563517/93‑1 (MQ=255)
                                      cAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATAccc                                                   <  2:834667/93‑1 (MQ=255)
                                       aaaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCg                                                  <  2:4305883/93‑1 (MQ=255)
                                        aaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCgg                                                 >  2:453933/1‑93 (MQ=255)
                                        aaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCgg                                                 >  1:2687407/1‑93 (MQ=255)
                                        aaCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCgg                                                 <  2:3749564/93‑1 (MQ=255)
                                         aCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCggg                                                <  1:94778/93‑1 (MQ=255)
                                            gACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCg                                             >  2:6707324/1‑93 (MQ=255)
                                            gACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCg                                             >  1:2545696/1‑93 (MQ=255)
                                            gACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCg                                             >  2:6406921/1‑93 (MQ=255)
                                             aCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGc                                            >  1:5934652/1‑93 (MQ=255)
                                               aaTTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTa                                          >  2:2775571/1‑93 (MQ=255)
                                                 ttttGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAAt                                        <  1:3620757/93‑1 (MQ=255)
                                                 ttttGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAAt                                        <  2:3439213/93‑1 (MQ=255)
                                                  tttGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAAtt                                       <  2:1152234/93‑1 (MQ=255)
                                                     gCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTcac                                    >  2:2753243/1‑93 (MQ=255)
                                                       tctTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACaa                                  <  2:4483362/93‑1 (MQ=255)
                                                        ctTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACaaa                                 <  1:525127/93‑1 (MQ=255)
                                                        ctTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATACACaaa                                 <  2:4776679/93‑1 (MQ=255)
                                                         ttCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAAt                                >  2:530906/1‑93 (MQ=255)
                                                          tCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATc                               >  1:6010379/1‑93 (MQ=255)
                                                           cGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCt                              <  1:2990004/93‑1 (MQ=255)
                                                            gAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTg                             >  2:2954737/1‑93 (MQ=255)
                                                             aGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgc                            >  2:6518467/1‑93 (MQ=255)
                                                             aGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgc                            <  1:6108215/93‑1 (MQ=255)
                                                              gTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcg                           >  2:1327886/1‑93 (MQ=255)
                                                              gTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcg                           >  2:4059851/1‑93 (MQ=255)
                                                               ttttGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgc                          <  2:1833501/93‑1 (MQ=255)
                                                               ttttGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgc                          <  2:3595540/93‑1 (MQ=255)
                                                                tttGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgcg                         <  1:1453763/93‑1 (MQ=255)
                                                                tttGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgcg                         >  1:680981/1‑93 (MQ=255)
                                                                  tGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGcgcgcg                       <  1:6276447/93‑1 (MQ=255)
                                                                   gCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGa                      <  2:2676844/93‑1 (MQ=255)
                                                                     aGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGAtt                    <  1:3896917/93‑1 (MQ=255)
                                                                      gCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTg                   >  2:3143492/1‑93 (MQ=255)
                                                                      gCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTg                   <  1:3534890/93‑1 (MQ=255)
                                                                        ctctGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCa                 >  2:1597768/1‑93 (MQ=255)
                                                                        ctctGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCa                 >  1:2813668/1‑93 (MQ=255)
                                                                        ctctGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCa                 >  2:4182818/1‑93 (MQ=255)
                                                                         tctGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAg                >  1:2935928/1‑93 (MQ=255)
                                                                         tctGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAg                >  2:5850952/1‑93 (MQ=255)
                                                                         tctGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAg                >  2:4438573/1‑93 (MQ=255)
                                                                           tgtgAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGa              <  1:531075/93‑1 (MQ=255)
                                                                           tgtgAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGa              <  2:6102522/93‑1 (MQ=255)
                                                                           tgtgAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGa              >  2:3900439/1‑93 (MQ=255)
                                                                           tgtgAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGa              >  2:5925041/1‑93 (MQ=255)
                                                                            gtgAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGAt             >  1:4943615/1‑93 (MQ=255)
                                                                             tgAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATg            <  2:2511194/93‑1 (MQ=255)
                                                                               aaaaTATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGtt          <  1:562234/93‑1 (MQ=255)
                                                                               aaaaTATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGtt          >  1:3771101/1‑93 (MQ=255)
                                                                                aaaTATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTc         >  2:2084674/1‑93 (MQ=255)
                                                                                aaaTATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTc         <  1:5717221/93‑1 (MQ=255)
                                                                                  atatTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCtt       <  2:5161304/93‑1 (MQ=255)
                                                                                   tatTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTg      <  2:1057494/93‑1 (MQ=255)
                                                                                   tatTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTg      >  2:1540233/1‑93 (MQ=255)
                                                                                     ttCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGta    <  2:5775792/93‑1 (MQ=255)
                                                                                     ttCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGta    >  1:4679938/1‑93 (MQ=255)
                                                                                     ttCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGta    >  2:7468370/1‑93 (MQ=255)
                                                                                     ttCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGta    <  1:3257901/93‑1 (MQ=255)
                                                                                      tCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGtat   >  1:7447641/1‑93 (MQ=255)
                                                                                       cATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGtata  <  2:6238521/93‑1 (MQ=255)
                                                                                       cATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGtata  <  2:4651994/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATA  >  NC_000913/3012171‑3012350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGC  >  NC_000913/3012168‑3012354
                                                                                             |                                                                                             
GCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATG                                                                                               <  SRR4302542.6935440/94‑1 (MQ=60)
GCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATG                                                                                               <  SRR4302542.2903860/94‑1 (MQ=60)
 CGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGC                                                                                              >  SRR4302542.225610/1‑94 (MQ=60)
 CGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGC                                                                                              >  SRR4302542.29192/1‑94 (MQ=60)
  GGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCC                                                                                             >  SRR4302542.4569595/1‑94 (MQ=60)
   GGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCA                                                                                            <  SRR4302542.653306/94‑1 (MQ=60)
   GGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCA                                                                                            <  SRR4302542.6298559/94‑1 (MQ=60)
     GAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAAC                                                                                          <  SRR4302542.5941283/94‑1 (MQ=60)
     GAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAAC                                                                                          <  SRR4302542.1658997/94‑1 (MQ=60)
      AGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACC                                                                                         <  SRR4302542.2947663/94‑1 (MQ=60)
       GTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCT                                                                                        <  SRR4302542.2930877/94‑1 (MQ=60)
       GTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCT                                                                                        <  SRR4302542.5709204/94‑1 (MQ=60)
       GTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCT                                                                                        <  SRR4302542.6320091/94‑1 (MQ=60)
        TCGAACCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTC                                                                                       >  SRR4302542.7576680/1‑94 (MQ=60)
         CGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCA                                                                                      <  SRR4302542.1253395/94‑1 (MQ=60)
         CGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCA                                                                                      >  SRR4302542.4987757/1‑94 (MQ=60)
           AGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAAT                                                                                    >  SRR4302542.5188072/1‑94 (MQ=60)
           AGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAAT                                                                                    <  SRR4302542.6710383/94‑1 (MQ=60)
           AGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAAT                                                                                    <  SRR4302542.6741233/94‑1 (MQ=60)
           AGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAAT                                                                                    >  SRR4302542.7127944/1‑94 (MQ=60)
           AGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAAT                                                                                    <  SRR4302542.1011377/94‑1 (MQ=60)
           AGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAAT                                                                                    <  SRR4302542.6792865/94‑1 (MQ=60)
             CCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGG                                                                                  >  SRR4302542.4323565/1‑94 (MQ=60)
               TCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGAT                                                                                <  SRR4302542.1459492/94‑1 (MQ=60)
               TCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGAT                                                                                <  SRR4302542.7270235/94‑1 (MQ=60)
               TCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGAT                                                                                >  SRR4302542.237924/1‑94 (MQ=60)
                CGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATA                                                                               >  SRR4302542.6177982/1‑94 (MQ=60)
                CGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATA                                                                               >  SRR4302542.1712739/1‑94 (MQ=60)
                 GGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATAT                                                                              >  SRR4302542.3742389/1‑94 (MQ=60)
                 GGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATAT                                                                              >  SRR4302542.770260/1‑94 (MQ=60)
                 GGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT                                                                              <  SRR4302542.4821227/94‑1 (MQ=60)
                  GCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATC                                                                             >  SRR4302542.3412721/1‑94 (MQ=60)
                  GCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATC                                                                             >  SRR4302542.7263632/1‑94 (MQ=60)
                   CTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCC                                                                            >  SRR4302542.2920944/1‑94 (MQ=60)
                   CTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCC                                                                            >  SRR4302542.4511360/1‑94 (MQ=60)
                   CTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCC                                                                            >  SRR4302542.252898/1‑94 (MQ=60)
                    TATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCT                                                                           <  SRR4302542.6944854/94‑1 (MQ=60)
                    TATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCT                                                                           <  SRR4302542.2690644/94‑1 (MQ=60)
                     ATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTT                                                                          >  SRR4302542.3182328/1‑94 (MQ=60)
                     ATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTT                                                                          >  SRR4302542.2755982/1‑94 (MQ=60)
                     ATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTT                                                                          >  SRR4302542.5087705/1‑94 (MQ=60)
                     ATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTT                                                                          >  SRR4302542.3616879/1‑94 (MQ=60)
                     ATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTAAAAATATTCATGCCAACCTCAATGGATATCCTT                                                                          <  SRR4302542.6233930/94‑1 (MQ=60)
                      TCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTT                                                                         <  SRR4302542.2792452/94‑1 (MQ=60)
                      TCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTT                                                                         <  SRR4302542.2073146/94‑1 (MQ=60)
                       CAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGACATCCTTTC                                                                        <  SRR4302542.42935/94‑1 (MQ=60)
                        AACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCA                                                                       >  SRR4302542.183938/1‑94 (MQ=60)
                        AACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCA                                                                       <  SRR4302542.1865697/94‑1 (MQ=60)
                         ACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAG                                                                      <  SRR4302542.1232028/94‑1 (MQ=60)
                         ACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAG                                                                      <  SRR4302542.1256986/94‑1 (MQ=60)
                         ACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAG                                                                      <  SRR4302542.5913445/94‑1 (MQ=60)
                         ACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAG                                                                      <  SRR4302542.609668/94‑1 (MQ=60)
                          CAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGT                                                                     <  SRR4302542.5279047/94‑1 (MQ=60)
                           AATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTA                                                                    <  SRR4302542.5170761/94‑1 (MQ=60)
                           AATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTA                                                                    >  SRR4302542.1037367/1‑94 (MQ=60)
                            ATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAA                                                                   <  SRR4302542.4484459/94‑1 (MQ=60)
                            ATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAA                                                                   >  SRR4302542.5539801/1‑94 (MQ=60)
                               TGCGCCATAGCAAACGGGCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCACCCTCAATGGATATCCTTTCAGTAACCC                                                                <  SRR4302542.5459552/94‑1 (MQ=60)
                                  GCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGA                                                             >  SRR4302542.2589192/1‑94 (MQ=60)
                                   CCATAGCAATCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAA                                                            <  SRR4302542.6460881/94‑1 (MQ=60)
                                    CATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAAT                                                           <  SRR4302542.4467162/94‑1 (MQ=60)
                                    CATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAAT                                                           >  SRR4302542.1029231/1‑94 (MQ=60)
                                    CATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAAT                                                           <  SRR4302542.5900015/94‑1 (MQ=60)
                                    CATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAAT                                                           <  SRR4302542.1742774/94‑1 (MQ=60)
                                      TAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATAC                                                         <  SRR4302542.4922666/94‑1 (MQ=60)
                                      TAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATAC                                                         <  SRR4302542.2942384/94‑1 (MQ=60)
                                       AGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACC                                                        >  SRR4302542.3422635/1‑94 (MQ=60)
                                        GCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCC                                                       <  SRR4302542.5617576/94‑1 (MQ=60)
                                         CAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCG                                                      <  SRR4302542.5995820/94‑1 (MQ=60)
                                          AAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGG                                                     >  SRR4302542.2713317/1‑94 (MQ=60)
                                          AAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGG                                                     >  SRR4302542.5314446/1‑94 (MQ=60)
                                            ACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGC                                                   <  SRR4302542.95585/94‑1 (MQ=60)
                                             CGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCC                                                  >  SRR4302542.2203345/1‑94 (MQ=60)
                                              GGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCG                                                 >  SRR4302542.2570368/1‑94 (MQ=60)
                                              GGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCG                                                 >  SRR4302542.6917080/1‑94 (MQ=60)
                                               GACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGC                                                >  SRR4302542.5992146/1‑94 (MQ=60)
                                                ACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCT                                               <  SRR4302542.5643388/94‑1 (MQ=60)
                                                ACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCT                                               <  SRR4302542.3793153/94‑1 (MQ=60)
                                                 CAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTA                                              <  SRR4302542.1660887/94‑1 (MQ=60)
                                                 CAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTA                                              >  SRR4302542.3475451/1‑94 (MQ=60)
                                                   ATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAAT                                            >  SRR4302542.4732076/1‑94 (MQ=60)
                                                   ATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAAT                                            >  SRR4302542.5011514/1‑94 (MQ=60)
                                                    TTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATT                                           <  SRR4302542.3654488/94‑1 (MQ=60)
                                                     TTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTC                                          >  SRR4302542.1939065/1‑94 (MQ=60)
                                                      TTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCA                                         <  SRR4302542.5446340/94‑1 (MQ=60)
                                                       TGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCAC                                        <  SRR4302542.928776/94‑1 (MQ=60)
                                                         CTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAA                                      >  SRR4302542.5676182/1‑94 (MQ=60)
                                                         CTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAA                                      <  SRR4302542.1499134/94‑1 (MQ=60)
                                                          TCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAA                                     >  SRR4302542.5100745/1‑94 (MQ=60)
                                                           CTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAAT                                    <  SRR4302542.529664/94‑1 (MQ=60)
                                                           CTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAAT                                    <  SRR4302542.958977/94‑1 (MQ=60)
                                                           CTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAAT                                    <  SRR4302542.3873278/94‑1 (MQ=60)
                                                            TTCGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATC                                   <  SRR4302542.5042656/94‑1 (MQ=60)
                                                            TTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATC                                   >  SRR4302542.6068578/1‑94 (MQ=60)
                                                              CGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTG                                 <  SRR4302542.3018621/94‑1 (MQ=60)
                                                              CGAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCC‑GAATACCCCGGCCGGTAATTtaacaatcttc                                >  SRR4302542.6910585/1‑83 (MQ=60)
                                                              CGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTG                                 >  SRR4302542.170222/1‑94 (MQ=60)
                                                               GAGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGC                                >  SRR4302542.2894342/1‑94 (MQ=60)
                                                                AGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCG                               >  SRR4302542.3120301/1‑94 (MQ=60)
                                                                AGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCG                               <  SRR4302542.4323627/94‑1 (MQ=60)
                                                                AGTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCG                               <  SRR4302542.6167332/94‑1 (MQ=60)
                                                                 GTTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGC                              <  SRR4302542.1578036/94‑1 (MQ=60)
                                                                  TTTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCG                             >  SRR4302542.388337/1‑94 (MQ=60)
                                                                  TTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCG                             >  SRR4302542.686861/1‑94 (MQ=60)
                                                                   TTTGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGC                            <  SRR4302542.1466307/94‑1 (MQ=60)
                                                                     TGCAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGA                          <  SRR4302542.6338308/94‑1 (MQ=60)
                                                                       CAGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATT                        <  SRR4302542.4360437/94‑1 (MQ=60)
                                                                        AGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTG                       >  SRR4302542.4568999/1‑94 (MQ=60)
                                                                        AGCCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTG                       >  SRR4302542.4772452/1‑94 (MQ=60)
                                                                        AGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTG                       >  SRR4302542.7466932/1‑94 (MQ=60)
                                                                        AGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTG                       <  SRR4302542.3935392/94‑1 (MQ=60)
                                                                         GCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGC                      <  SRR4302542.5916973/94‑1 (MQ=60)
                                                                         GCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGC                      <  SRR4302542.3567926/94‑1 (MQ=60)
                                                                          CCTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCA                     >  SRR4302542.2840742/1‑94 (MQ=60)
                                                                          CCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCA                     >  SRR4302542.3597141/1‑94 (MQ=60)
                                                                           CTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAG                    >  SRR4302542.2964045/1‑94 (MQ=60)
                                                                           CTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAG                    >  SRR4302542.7458324/1‑94 (MQ=60)
                                                                           CTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAG                    >  SRR4302542.2043073/1‑94 (MQ=60)
                                                                           CTCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAG                    >  SRR4302542.5111102/1‑94 (MQ=60)
                                                                            TCTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGG                   >  SRR4302542.4321229/1‑94 (MQ=60)
                                                                             CTGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGA                  >  SRR4302542.3015650/1‑94 (MQ=60)
                                                                              TGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGAT                 >  SRR4302542.4991649/1‑94 (MQ=60)
                                                                              TGTGAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGAT                 <  SRR4302542.535661/94‑1 (MQ=60)
                                                                               GTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATG                <  SRR4302542.861324/94‑1 (MQ=60)
                                                                                 GAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTT              <  SRR4302542.1225626/94‑1 (MQ=60)
                                                                                 GAAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTT              >  SRR4302542.3808394/1‑94 (MQ=60)
                                                                                  AAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTC             <  SRR4302542.567078/94‑1 (MQ=60)
                                                                                  AAAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTC             >  SRR4302542.6016571/1‑94 (MQ=60)
                                                                                   AAATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCT            <  SRR4302542.5772709/94‑1 (MQ=60)
                                                                                    AATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTT           <  SRR4302542.4317829/94‑1 (MQ=60)
                                                                                     ATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTG          <  SRR4302542.5755093/94‑1 (MQ=60)
                                                                                     ATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTG          <  SRR4302542.3457/94‑1 (MQ=60)
                                                                                     ATATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTG          >  SRR4302542.3955975/1‑94 (MQ=60)
                                                                                      TATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCAATTGCAGGATGTTCTTGT         <  SRR4302542.5571072/94‑1 (MQ=60)
                                                                                       ATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTA        >  SRR4302542.6201433/1‑94 (MQ=60)
                                                                                       ATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTA        >  SRR4302542.5378785/1‑94 (MQ=60)
                                                                                       ATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTA        >  SRR4302542.4725668/1‑94 (MQ=60)
                                                                                       ATTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCCGCGCGCGATTACAGGATGTTCTTGTA        <  SRR4302542.4231429/94‑1 (MQ=60)
                                                                                        TTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTAT       <  SRR4302542.2382806/94‑1 (MQ=60)
                                                                                        TTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTAT       >  SRR4302542.2972985/1‑94 (MQ=60)
                                                                                        TTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTAT       >  SRR4302542.5835992/1‑94 (MQ=60)
                                                                                        TTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTAT       <  SRR4302542.3288672/94‑1 (MQ=60)
                                                                                        TTCATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTAT       >  SRR4302542.7520560/1‑94 (MQ=60)
                                                                                           ATGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATC    >  SRR4302542.760954/1‑94 (MQ=60)
                                                                                            TGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCG   <  SRR4302542.2473670/94‑1 (MQ=60)
                                                                                            TGCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCG   <  SRR4302542.4855336/94‑1 (MQ=60)
                                                                                             GCCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGC  <  SRR4302542.4468984/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGGGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGC  >  NC_000913/3012168‑3012354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑