Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,195,775 A→G V503A (GTG→GCG)  glnE ← GlnE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,195,7750AG83.3% 513.9 / 91.4 233V503A (GTG→GCG) glnEGlnE
Reads supporting (aligned to +/- strand):  ref base A (17/22);  new base G (82/112);  total (99/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

CACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTA  >  NC_000913/3195684‑3195867
                                                                                           |                                                                                            
    aGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGa                                                                                         <  1:2490741/93‑1 (MQ=255)
    aGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGa                                                                                         <  2:2579368/93‑1 (MQ=255)
       aaGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGt                                                                                      <  1:3468978/93‑1 (MQ=255)
        aGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTc                                                                                     >  2:4983953/1‑93 (MQ=255)
        aGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTc                                                                                     >  1:2957443/1‑93 (MQ=255)
         ggCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTcc                                                                                    <  2:7470584/93‑1 (MQ=255)
         ggCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTcc                                                                                    >  2:1903308/1‑93 (MQ=255)
         ggCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTcc                                                                                    <  1:4155562/93‑1 (MQ=255)
          gCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCt                                                                                   <  1:7281043/93‑1 (MQ=255)
          gCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCt                                                                                   >  1:1324815/1‑93 (MQ=255)
          gCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCt                                                                                   >  1:6895184/1‑93 (MQ=255)
            ggTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcg                                                                                 <  2:5592243/93‑1 (MQ=255)
             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                <  1:6140090/93‑1 (MQ=255)
             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                <  1:852430/93‑1 (MQ=255)
             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                <  2:6041264/93‑1 (MQ=255)
             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                <  2:7205386/93‑1 (MQ=255)
             gTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgc                                                                                >  1:604913/1‑93 (MQ=255)
              tAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTcgcg                                                                               >  2:3550542/1‑93 (MQ=255)
              tAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTcgcg                                                                               >  1:4575827/1‑93 (MQ=255)
                 tGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGcc                                                                            >  1:6668868/1‑93 (MQ=255)
                 tGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGcc                                                                            <  2:3878775/93‑1 (MQ=255)
                 tGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGcc                                                                            <  1:3192404/93‑1 (MQ=255)
                 tGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGcc                                                                            <  2:7247535/93‑1 (MQ=255)
                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         >  2:2568894/1‑93 (MQ=255)
                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         <  1:795123/93‑1 (MQ=255)
                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         <  2:1189973/93‑1 (MQ=255)
                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         <  1:2532916/93‑1 (MQ=255)
                    gcgACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGa                                                                         <  1:2075886/93‑1 (MQ=255)
                      gACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGTGGCCCGATg                                                                       >  2:3496777/1‑93 (MQ=255)
                       aCAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATgg                                                                      <  2:1255030/93‑1 (MQ=255)
                       aCAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATgg                                                                      <  1:1231998/93‑1 (MQ=255)
                       aCAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATgg                                                                      <  2:953197/93‑1 (MQ=255)
                        cAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGt                                                                     <  1:1262417/93‑1 (MQ=255)
                        cAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGt                                                                     <  1:92300/93‑1 (MQ=255)
                          gCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTgc                                                                   <  1:2306015/93‑1 (MQ=255)
                            gTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTgcgc                                                                 >  2:144070/1‑93 (MQ=255)
                             tAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCt                                                                >  1:5615069/1‑93 (MQ=255)
                             tAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCt                                                                <  1:2142963/93‑1 (MQ=255)
                              aaCGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCtt                                                               <  2:658545/93‑1 (MQ=255)
                              aaCGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCtt                                                               >  2:1161460/1‑93 (MQ=255)
                                 ggCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATc                                                            <  1:135744/93‑1 (MQ=255)
                                 ggCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATc                                                            <  1:4731209/93‑1 (MQ=255)
                                     gCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGc                                                        >  1:2217900/1‑93 (MQ=255)
                                     gCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGc                                                        <  1:3337721/93‑1 (MQ=255)
                                        tCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGctct                                                     >  1:4049269/1‑93 (MQ=255)
                                        tCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGctct                                                     >  1:1882867/1‑93 (MQ=255)
                                          ttCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCttt                                                   >  1:1518836/1‑93 (MQ=255)
                                          ttCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCttt                                                   <  1:3649916/93‑1 (MQ=255)
                                           tCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTg                                                  <  1:4810494/93‑1 (MQ=255)
                                             aCGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCg                                                <  1:4987386/93‑1 (MQ=255)
                                             aCGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCg                                                >  1:7495195/1‑93 (MQ=255)
                                             aCGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCg                                                >  1:6942129/1‑93 (MQ=255)
                                               gcgcACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGa                                              >  1:5661516/1‑93 (MQ=255)
                                                 gcACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGTCCCGATGGTGCGCTTATCCAGCTCTTTGCGGaaa                                            <  2:2702074/93‑1 (MQ=255)
                                                 gcACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGaaa                                            <  1:2855007/93‑1 (MQ=255)
                                                   acaGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATc                                          <  2:1346640/93‑1 (MQ=255)
                                                   acaGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATc                                          <  2:2973040/93‑1 (MQ=255)
                                                    caGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCg                                         >  1:430614/1‑93 (MQ=255)
                                                      gACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGc                                       >  1:679548/1‑93 (MQ=255)
                                                      gACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGc                                       <  2:3300633/93‑1 (MQ=255)
                                                        cATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCaa                                     >  1:4116180/1‑93 (MQ=255)
                                                        cATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCaa                                     <  2:5060038/93‑1 (MQ=255)
                                                         aTCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAAt                                    <  2:671860/93‑1 (MQ=255)
                                                         aTCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAAt                                    <  2:2885072/93‑1 (MQ=255)
                                                         aTCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAAt                                    >  2:3163322/1‑93 (MQ=255)
                                                          tCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATc                                   >  1:1576782/1‑93 (MQ=255)
                                                          tCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATc                                   <  1:244516/93‑1 (MQ=255)
                                                          tCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATc                                   <  1:2682036/93‑1 (MQ=255)
                                                           cACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCa                                  >  1:1132696/1‑93 (MQ=255)
                                                            aCTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAg                                 >  1:5166615/1‑93 (MQ=255)
                                                            aCTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAg                                 >  1:6527562/1‑93 (MQ=255)
                                                            aCTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCAGCAATCAg                                 <  1:3767070/93‑1 (MQ=255)
                                                             cTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGc                                <  2:6197952/93‑1 (MQ=255)
                                                             cTTAGCAGATGCGGCATCAGATGGACGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGc                                <  2:4788503/93‑1 (MQ=255)
                                                               tAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGt                              >  1:3034164/1‑93 (MQ=255)
                                                                aGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGtt                             <  2:2069750/93‑1 (MQ=255)
                                                                 gCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTa                            <  2:5680359/93‑1 (MQ=255)
                                                                  cAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAg                           <  1:7177642/93‑1 (MQ=255)
                                                                  cAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAg                           <  2:5312072/93‑1 (MQ=255)
                                                                     aTGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGcgc                        <  2:5957638/93‑3 (MQ=255)
                                                                      tGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGcc                       <  1:3821651/93‑1 (MQ=255)
                                                                         ggCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGt                    <  2:4405038/93‑1 (MQ=255)
                                                                         ggCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGt                    >  2:4029894/1‑93 (MQ=255)
                                                                          gCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGtt                   <  2:2599592/93‑1 (MQ=255)
                                                                          gCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGtt                   <  1:5292412/93‑1 (MQ=255)
                                                                          gCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGtt                   <  2:163541/93‑1 (MQ=255)
                                                                           cATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGttt                  <  1:6407472/93‑1 (MQ=255)
                                                                             tCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGc                >  2:2785734/1‑93 (MQ=255)
                                                                             tCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGc                <  2:4612022/93‑1 (MQ=255)
                                                                             tCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGc                >  2:4190321/1‑93 (MQ=255)
                                                                              cAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCg               <  2:5473746/93‑1 (MQ=255)
                                                                               aGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGa              >  2:1658584/1‑93 (MQ=255)
                                                                                 aTGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATGAGCGTTAGCGCCTGTTTGCGatc            >  2:6971093/1‑93 (MQ=255)
                                                                                  tGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatca           <  2:2335441/93‑1 (MQ=255)
                                                                                   ggTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGatcat          <  2:7269726/93‑1 (MQ=255)
                                                                                    gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc         <  2:1585069/93‑1 (MQ=255)
                                                                                    gTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGatcatc         >  1:3137161/1‑93 (MQ=255)
                                                                                       gAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTc      <  2:2845184/93‑1 (MQ=255)
                                                                                        aGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCg     >  1:6247971/1‑93 (MQ=255)
                                                                                           aCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCCGTTTGCGATCATCCTCGCTa  >  1:5400581/1‑93 (MQ=255)
                                                                                           |                                                                                            
CACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTA  >  NC_000913/3195684‑3195867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAA  >  NC_000913/3195684‑3195868
                                                                                           |                                                                                             
CACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACC                                                                                             <  SRR4302542.1710403/94‑1 (MQ=60)
 ACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCT                                                                                            <  SRR4302542.2581469/94‑1 (MQ=60)
  CCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTG                                                                                           <  SRR4302542.4109527/94‑1 (MQ=60)
   CAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGA                                                                                          <  SRR4302542.6324017/94‑1 (MQ=60)
    AGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGAC                                                                                         <  SRR4302542.2514906/94‑1 (MQ=60)
    AGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGAC                                                                                         <  SRR4302542.4766003/94‑1 (MQ=60)
    AGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGAC                                                                                         <  SRR4302542.6968931/94‑1 (MQ=60)
     GCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACG                                                                                        >  SRR4302542.5403817/1‑94 (MQ=60)
      CAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGT                                                                                       >  SRR4302542.5110136/1‑94 (MQ=60)
       AAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTC                                                                                      <  SRR4302542.3501458/94‑1 (MQ=60)
       AAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTC                                                                                      >  SRR4302542.2985790/1‑94 (MQ=60)
       AAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTC                                                                                      >  SRR4302542.2730411/1‑94 (MQ=60)
       AAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACCCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTC                                                                                      >  SRR4302542.6266514/1‑94 (MQ=60)
         GGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCT                                                                                    <  SRR4302542.4196470/94‑1 (MQ=60)
         GGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCT                                                                                    >  SRR4302542.1336224/1‑94 (MQ=60)
         GGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCT                                                                                    >  SRR4302542.6962895/1‑94 (MQ=60)
          GCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTC                                                                                   <  SRR4302542.7352445/94‑1 (MQ=60)
            GGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGC                                                                                 >  SRR4302542.610121/1‑94 (MQ=60)
            GGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGC                                                                                 >  SRR4302542.2555851/1‑94 (MQ=60)
             GTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCG                                                                                <  SRR4302542.6199503/94‑1 (MQ=60)
             GTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCG                                                                                <  SRR4302542.859778/94‑1 (MQ=60)
             GTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCG                                                                                <  SRR4302542.6960797/94‑1 (MQ=60)
             GTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCG                                                                                >  SRR4302542.4620596/1‑94 (MQ=60)
                ATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCC                                                                             >  SRR4302542.6734574/1‑94 (MQ=60)
                 TGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCC                                                                            <  SRR4302542.3222637/94‑1 (MQ=60)
                    GCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGAT                                                                         <  SRR4302542.2093819/94‑1 (MQ=60)
                    GCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGAT                                                                         <  SRR4302542.801968/94‑1 (MQ=60)
                    GCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGAT                                                                         <  SRR4302542.2557489/94‑1 (MQ=60)
                      GACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGG                                                                       >  SRR4302542.2694828/1‑94 (MQ=60)
                       ACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGT                                                                      <  SRR4302542.1242593/94‑1 (MQ=60)
                       ACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGT                                                                      >  SRR4302542.86810/1‑94 (MQ=60)
                        CAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTG                                                                     <  SRR4302542.93074/94‑1 (MQ=60)
                        CAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTG                                                                     <  SRR4302542.1273270/94‑1 (MQ=60)
                         AGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGC                                                                    <  SRR4302542.2488659/94‑1 (MQ=60)
                          GCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCG                                                                   <  SRR4302542.6463409/94‑1 (MQ=60)
                          GCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCG                                                                   <  SRR4302542.2325819/94‑1 (MQ=60)
                            GTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCT                                                                 >  SRR4302542.5669619/1‑94 (MQ=60)
                             TAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTT                                                                <  SRR4302542.2161449/94‑1 (MQ=60)
                             TAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTT                                                                <  SRR4302542.1343667/94‑1 (MQ=60)
                               ACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTAT                                                              <  SRR4302542.2412554/94‑1 (MQ=60)
                               ACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTAT                                                              <  SRR4302542.3282938/94‑1 (MQ=60)
                                 GGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCC                                                            <  SRR4302542.4777346/94‑1 (MQ=60)
                                 GGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCC                                                            >  SRR4302542.7051506/1‑94 (MQ=60)
                                 GGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCC                                                            <  SRR4302542.136882/94‑1 (MQ=60)
                                 GGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCC                                                            >  SRR4302542.4409158/1‑94 (MQ=60)
                                  GCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCA                                                           <  SRR4302542.654715/94‑1 (MQ=60)
                                  GCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCA                                                           <  SRR4302542.3613889/94‑1 (MQ=60)
                                   CAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAG                                                          >  SRR4302542.1315512/1‑94 (MQ=60)
                                    AGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGC                                                         >  SRR4302542.4335252/1‑94 (MQ=60)
                                    AGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGC                                                         >  SRR4302542.2236981/1‑94 (MQ=60)
                                     GCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCT                                                        <  SRR4302542.3369132/94‑1 (MQ=60)
                                      CGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTC                                                       >  SRR4302542.3160927/1‑94 (MQ=60)
                                      CGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTC                                                       >  SRR4302542.4998854/1‑94 (MQ=60)
                                       GTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCT                                                      >  SRR4302542.1899164/1‑94 (MQ=60)
                                       GTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCT                                                      >  SRR4302542.2092832/1‑94 (MQ=60)
                                       GTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCT                                                      <  SRR4302542.2970111/94‑1 (MQ=60)
                                       GTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCT                                                      >  SRR4302542.4089214/1‑94 (MQ=60)
                                         CTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTT                                                    >  SRR4302542.669714/1‑94 (MQ=60)
                                         CTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTT                                                    >  SRR4302542.1531957/1‑94 (MQ=60)
                                          TTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTG                                                   <  SRR4302542.3684006/94‑1 (MQ=60)
                                           TCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGC                                                  <  SRR4302542.4857350/94‑1 (MQ=60)
                                            CACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCG                                                 >  SRR4302542.7010339/1‑94 (MQ=60)
                                            CACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCG                                                 >  SRR4302542.7568807/1‑94 (MQ=60)
                                             ACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGG                                                <  SRR4302542.5035781/94‑1 (MQ=60)
                                              CGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGA                                               >  SRR4302542.5716514/1‑94 (MQ=60)
                                               GCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAA                                              <  SRR4302542.5737003/94‑1 (MQ=60)
                                                CGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAA                                             >  SRR4302542.2309768/1‑94 (MQ=60)
                                                 GCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAAT                                            <  SRR4302542.2882445/94‑1 (MQ=60)
                                                   ACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCG                                          >  SRR4302542.434339/1‑94 (MQ=60)
                                                   ACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCG                                          >  SRR4302542.795364/1‑94 (MQ=60)
                                                    CAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGG                                         <  SRR4302542.5309244/94‑1 (MQ=60)
                                                     AGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGC                                        >  SRR4302542.685415/1‑94 (MQ=60)
                                                     AGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGC                                        >  SRR4302542.344249/1‑94 (MQ=60)
                                                      GACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCA                                       <  SRR4302542.4842212/94‑1 (MQ=60)
                                                       ACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAA                                      >  SRR4302542.4156738/1‑94 (MQ=60)
                                                        CATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAAT                                     <  SRR4302542.1283920/94‑1 (MQ=60)
                                                         ATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATC                                    >  SRR4302542.2196943/1‑94 (MQ=60)
                                                         ATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATC                                    >  SRR4302542.1590397/1‑94 (MQ=60)
                                                         ATCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATC                                    >  SRR4302542.7297338/1‑94 (MQ=60)
                                                          TCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCA                                   <  SRR4302542.246615/94‑1 (MQ=60)
                                                          TCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCA                                   <  SRR4302542.2707909/94‑1 (MQ=60)
                                                          TCACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCA                                   <  SRR4302542.3015083/94‑1 (MQ=60)
                                                          TCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCA                                   >  SRR4302542.1142392/1‑94 (MQ=60)
                                                           CACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAG                                  >  SRR4302542.5216828/1‑94 (MQ=60)
                                                           CACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAG                                  >  SRR4302542.6591890/1‑94 (MQ=60)
                                                           CACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAG                                  <  SRR4302542.2370002/94‑1 (MQ=60)
                                                           CACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAG                                  <  SRR4302542.3874515/94‑1 (MQ=60)
                                                            ACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGC                                 >  SRR4302542.44034/1‑94 (MQ=60)
                                                            ACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGC                                 <  SRR4302542.2144083/94‑1 (MQ=60)
                                                            ACTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCAGCAATCAGC                                 <  SRR4302542.3804330/94‑1 (MQ=60)
                                                             CTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATaagct                                >  SRR4302542.7556115/1‑89 (MQ=60)
                                                             CTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCG                                <  SRR4302542.3349518/94‑1 (MQ=60)
                                                             CTTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCG                                >  SRR4302542.2143876/1‑94 (MQ=60)
                                                              TTAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGT                               >  SRR4302542.3063112/1‑94 (MQ=60)
                                                               TAGCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTT                              <  SRR4302542.1262928/94‑1 (MQ=60)
                                                                AGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTA                             <  SRR4302542.1563018/94‑1 (MQ=60)
                                                                 GCAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAG                            <  SRR4302542.879415/94‑1 (MQ=60)
                                                                 GCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAG                            <  SRR4302542.3806340/94‑1 (MQ=60)
                                                                  CAGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGC                           <  SRR4302542.7248049/94‑1 (MQ=60)
                                                                  CAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGC                           >  SRR4302542.3730117/1‑94 (MQ=60)
                                                                   AGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCG                          >  SRR4302542.3777064/1‑94 (MQ=60)
                                                                   AGATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCG                          >  SRR4302542.6122320/1‑94 (MQ=60)
                                                                    GATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGC                         >  SRR4302542.1564626/1‑94 (MQ=60)
                                                                     ATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCC                        >  SRR4302542.5496370/1‑94 (MQ=60)
                                                                     ATGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCC                        >  SRR4302542.7402304/1‑94 (MQ=60)
                                                                     ATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACC                        <  SRR4302542.3190054/94‑1 (MQ=60)
                                                                      TGCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCT                       <  SRR4302542.3859462/94‑1 (MQ=60)
                                                                       gccgccataaATGGTCCAGCGCCTGACGTCCTCGCGGCCCGATTGTGCGCTTATTCCGCTCTTTGCGGAAATCGGgaaaccgcctttagcgcct                      <  SRR4302542.3690173/84‑20 (MQ=47)
                                                                       GCGGCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTG                      <  SRR4302542.4950698/94‑1 (MQ=60)
                                                                        CGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGT                     >  SRR4302542.6819498/1‑94 (MQ=60)
                                                                          GCATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTT                   <  SRR4302542.5343849/94‑1 (MQ=60)
                                                                           CATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTG                  <  SRR4302542.6470607/94‑1 (MQ=60)
                                                                           CATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTG                  >  SRR4302542.5753977/1‑94 (MQ=60)
                                                                            ATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGC                 <  SRR4302542.1439831/94‑1 (MQ=60)
                                                                            ATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGC                 >  SRR4302542.4135437/1‑94 (MQ=60)
                                                                            ATCAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGC                 >  SRR4302542.5446939/1‑94 (MQ=60)
                                                                             TCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCG                >  SRR4302542.6838872/1‑94 (MQ=60)
                                                                              CAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGA               <  SRR4302542.853931/94‑1 (MQ=60)
                                                                              CAGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGA               >  SRR4302542.5707443/1‑94 (MQ=60)
                                                                               AGATGGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGAT              >  SRR4302542.4170872/1‑94 (MQ=60)
                                                                                   GGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATC          >  SRR4302542.3166980/1‑94 (MQ=60)
                                                                                   GGTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATC          >  SRR4302542.1182927/1‑94 (MQ=60)
                                                                                    GTCGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATGATCC         <  SRR4302542.7041981/94‑1 (MQ=60)
                                                                                      CGAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTC       <  SRR4302542.3043636/94‑1 (MQ=60)
                                                                                       GAGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCG      >  SRR4302542.6309538/1‑94 (MQ=60)
                                                                                        AGCGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGC     >  SRR4302542.207670/1‑94 (MQ=60)
                                                                                          CGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTA   >  SRR4302542.1493077/1‑94 (MQ=60)
                                                                                          CGCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTA   >  SRR4302542.2045996/1‑94 (MQ=60)
                                                                                          CACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCCGTTTGCGATCATCCTCGCTA   >  SRR4302542.5453046/1‑94 (MQ=60)
                                                                                           GCCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCGCCTGTTTGCGATCATCCTCGCTAA  <  SRR4302542.6821484/94‑1 (MQ=60)
                                                                                           |                                                                                             
CACCAGCAAGGCGGTAATGCGCGACAGCGTAACGGCAGCGTCTTCACGCGCACAGACATCACTTAGCAGATGCGGCATCAGATGGTCGAGCACCTGACGTCCTCGCGGCCCGATGGTGCGCTTATCCAGCTCTTTGCGGAAATCGGCAATCAGCGTTAGCACCTGTTTGCGATCATCCTCGCTAA  >  NC_000913/3195684‑3195868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑