Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,483,905 T→C V50A (GTG→GCG)  crp → cyclic AMP receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,483,9050TC82.2% 644.3 / 140.9 304V50A (GTG→GCG) crpcyclic AMP receptor protein
Reads supporting (aligned to +/- strand):  ref base T (18/36);  new base C (103/147);  total (121/183)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 307 total aligned reads displayed.

ACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCC  >  NC_000913/3483815‑3483997
                                                                                           |                                                                                            
 caTTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCt                                                                                           >  2:6547739/1‑93 (MQ=255)
  aTTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTg                                                                                          <  2:4989834/93‑1 (MQ=255)
   ttCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGa                                                                                         <  2:1239056/93‑1 (MQ=255)
   ttCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGa                                                                                         <  1:386435/93‑1 (MQ=255)
   ttCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGa                                                                                         <  2:4024169/93‑1 (MQ=255)
       tAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCaa                                                                                     >  1:1744871/1‑93 (MQ=255)
       tAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCaa                                                                                     <  2:7122452/93‑1 (MQ=255)
        aaGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCaaa                                                                                    <  2:5462639/93‑1 (MQ=255)
          gTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGa                                                                                  >  1:7453812/1‑93 (MQ=255)
           tACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGAc                                                                                 <  1:6368549/93‑1 (MQ=255)
           tACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGAc                                                                                 >  2:5739406/1‑93 (MQ=255)
             cccATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGa                                                                               >  1:3263377/1‑93 (MQ=255)
             cccATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGa                                                                               >  2:3173654/1‑93 (MQ=255)
              ccATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGaa                                                                              <  1:6797191/93‑1 (MQ=255)
              ccATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGaa                                                                              <  2:1124025/93‑1 (MQ=255)
                aTCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAaga                                                                            <  1:4340949/93‑1 (MQ=255)
                aTCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAaga                                                                            <  2:260001/93‑1 (MQ=255)
                 tCCAAGAGCACGCTTATTTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCCCTGATCAAAGACGAaga                                                                            <  1:2373694/93‑1 (MQ=255)
                 tCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAagag                                                                           <  2:1693193/93‑1 (MQ=255)
                 tCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAagag                                                                           >  2:6066332/1‑93 (MQ=255)
                  ccAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAgg                                                                          >  1:2488902/1‑93 (MQ=255)
                  ccAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAgg                                                                          <  2:6775608/93‑1 (MQ=255)
                   cAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAggg                                                                         >  2:6981239/1‑93 (MQ=255)
                   cAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAggg                                                                         >  1:5886274/1‑93 (MQ=255)
                     agagCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTa                                                                       >  2:1879872/1‑93 (MQ=255)
                      gagCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTaa                                                                      <  1:304835/93‑1 (MQ=255)
                      gagCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGGaa                                                                      >  1:1288923/1‑93 (MQ=255)
                       agCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTaaa                                                                     <  2:6398150/93‑1 (MQ=255)
                        gCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAg                                                                    <  2:3793665/93‑1 (MQ=255)
                         cACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGa                                                                   <  1:692484/93‑1 (MQ=255)
                          aCGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGaa                                                                  >  1:6649748/1‑93 (MQ=255)
                          aCGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGaa                                                                  >  1:5556741/1‑93 (MQ=255)
                           cGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGaaa                                                                 <  2:2407154/93‑1 (MQ=255)
                           cGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGaaa                                                                 <  2:5308048/93‑1 (MQ=255)
                            gCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAAt                                                                >  1:5478532/1‑93 (MQ=255)
                             cTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATg                                                               >  1:2747298/1‑93 (MQ=255)
                             cTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATg                                                               >  1:123855/1‑93 (MQ=255)
                              ttA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGa                                                              <  2:4902157/93‑1 (MQ=255)
                                a‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATc                                                            >  2:1504445/1‑93 (MQ=255)
                                  ttCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                                           >  2:1000017/1‑93 (MQ=255)
                                  ttCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                                           <  2:3546817/93‑1 (MQ=255)
                                   tCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCct                                                          <  1:2978949/93‑1 (MQ=255)
                                   tCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCct                                                          >  2:4161575/1‑93 (MQ=255)
                                    cACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCctc                                                         <  1:1050184/93‑1 (MQ=255)
                                    cACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCctc                                                         >  2:4495162/1‑93 (MQ=255)
                                       cAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTcc                                                      <  2:4592687/93‑1 (MQ=255)
                                        aGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCt                                                     >  1:2046188/1‑93 (MQ=255)
                                        aGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCt                                                     <  1:1623718/93‑1 (MQ=255)
                                         gggTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTa                                                    <  1:2233320/93‑1 (MQ=255)
                                         gggTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTa                                                    >  1:1386278/1‑93 (MQ=255)
                                           gTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATc                                                  <  2:3739626/93‑1 (MQ=255)
                                           gTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATc                                                  <  1:5680949/93‑1 (MQ=255)
                                            tGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                                                 <  1:5738416/93‑1 (MQ=255)
                                            tGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                                                 >  1:3533320/1‑93 (MQ=255)
                                             gAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTg                                                <  2:3374713/93‑1 (MQ=255)
                                              aaaaaGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGa                                               <  2:4356591/93‑1 (MQ=255)
                                                  aGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCa                                           <  2:1371117/93‑1 (MQ=255)
                                                   gCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAg                                          <  2:7389269/93‑1 (MQ=255)
                                                     ggAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAggg                                        <  2:267085/93‑1 (MQ=255)
                                                      gAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGt                                       >  2:3810077/1‑93 (MQ=255)
                                                      gAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGt                                       >  2:47939/1‑93 (MQ=255)
                                                      gAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGt                                       <  2:2224812/93‑1 (MQ=255)
                                                        aaCGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGa                                     <  1:3850049/93‑1 (MQ=255)
                                                           gCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAttt                                  >  1:689391/1‑93 (MQ=255)
                                                             tGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTa                                >  2:1072639/1‑93 (MQ=255)
                                                             tGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTa                                <  2:2025540/93‑1 (MQ=255)
                                                             tGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTa                                <  1:7263752/93‑1 (MQ=255)
                                                              gTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAt                               >  1:3440926/1‑93 (MQ=255)
                                                               tactacATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                              <  1:3303577/93‑1 (MQ=255)
                                                               tactacATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                              >  1:7100842/1‑93 (MQ=255)
                                                                 ctacATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTgg                            <  1:457018/93‑1 (MQ=255)
                                                                  tacATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc                           <  2:2947434/93‑1 (MQ=255)
                                                                  tacATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGc                           >  1:4073233/1‑93 (MQ=255)
                                                                   acATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCg                          >  2:4816296/1‑93 (MQ=255)
                                                                   acATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCg                          <  1:1077618/93‑1 (MQ=255)
                                                                     aTCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGaa                        <  2:3273405/93‑1 (MQ=255)
                                                                     aTCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGaa                        >  1:529240/1‑93 (MQ=255)
                                                                      tCGTTATAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAAc                       >  2:6650548/1‑93 (MQ=255)
                                                                      tCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAAc                       >  2:4427937/1‑93 (MQ=255)
                                                                       cGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACt                      <  2:5630415/93‑1 (MQ=255)
                                                                       cGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACt                      <  1:79284/93‑1 (MQ=255)
                                                                       cGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACt                      <  1:4837900/93‑1 (MQ=255)
                                                                         ttAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTgg                    >  1:5086048/1‑93 (MQ=255)
                                                                         ttAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTgg                    <  1:3350742/93‑1 (MQ=255)
                                                                            aaGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGcc                 >  1:3753022/1‑93 (MQ=255)
                                                                            aaGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGcc                 >  1:621964/1‑93 (MQ=255)
                                                                              ggCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTg               >  2:4668521/1‑93 (MQ=255)
                                                                               gCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGt              >  1:6613959/1‑93 (MQ=255)
                                                                                 tctGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGttt            <  2:1940945/93‑1 (MQ=255)
                                                                                 tctGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGttt            >  2:6356277/1‑93 (MQ=255)
                                                                                   tgtgGCAGCACTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGa          <  1:4179859/93‑1 (MQ=255)
                                                                                    gtgGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGaa         <  1:5152925/93‑1 (MQ=255)
                                                                                    gtgGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGaa         <  1:2832760/93‑1 (MQ=255)
                                                                                     tgGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAag        <  1:2639701/93‑1 (MQ=255)
                                                                                      ggCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAaga       <  1:7350681/93‑1 (MQ=255)
                                                                                      ggCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAaga       <  1:4265301/93‑1 (MQ=255)
                                                                                      ggCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAaga       <  1:1486726/93‑1 (MQ=255)
                                                                                       gCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAagag      <  2:2275707/93‑1 (MQ=255)
                                                                                          gTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGc   <  2:3393083/93‑1 (MQ=255)
                                                                                           tGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCCGTTTGAAGAGGGcc  <  2:7485290/93‑1 (MQ=255)
                                                                                           |                                                                                            
ACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCC  >  NC_000913/3483815‑3483997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCA  >  NC_000913/3483812‑3483998
                                                                                              |                                                                                             
GCCACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGC                                                                                               >  SRR4302542.251565/1‑94 (MQ=60)
GCCACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGC                                                                                               <  SRR4302542.6931/94‑1 (MQ=60)
 CCACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCG                                                                                              >  SRR4302542.2836281/1‑94 (MQ=60)
 CCACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCG                                                                                              >  SRR4302542.1753883/1‑94 (MQ=60)
 CCACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCG                                                                                              >  SRR4302542.1239623/1‑94 (MQ=60)
  CACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGC                                                                                             >  SRR4302542.653424/1‑94 (MQ=60)
  CACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGC                                                                                             <  SRR4302542.4241366/94‑1 (MQ=60)
   ACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCT                                                                                            <  SRR4302542.3902201/94‑1 (MQ=60)
   ACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCT                                                                                            >  SRR4302542.5167825/1‑94 (MQ=60)
      TTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGAT                                                                                         <  SRR4302542.991735/94‑1 (MQ=60)
      TTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGAT                                                                                         <  SRR4302542.389789/94‑1 (MQ=60)
      TTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGGGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGAT                                                                                         >  SRR4302542.6281327/1‑94 (MQ=60)
         ATAAGTACCCATCCAAGAGCATGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAA                                                                                      >  SRR4302542.5756069/1‑94 (MQ=60)
         ATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAA                                                                                      >  SRR4302542.2845316/1‑94 (MQ=60)
         ATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAA                                                                                      >  SRR4302542.1760009/1‑94 (MQ=60)
          TAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAA                                                                                     <  SRR4302542.2520548/94‑1 (MQ=60)
            AGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGA                                                                                   >  SRR4302542.7526802/1‑94 (MQ=60)
            AGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGA                                                                                   <  SRR4302542.5640692/94‑1 (MQ=60)
             GTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGAC                                                                                  >  SRR4302542.3681687/1‑94 (MQ=60)
              TACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACG                                                                                 <  SRR4302542.6431265/94‑1 (MQ=60)
               ACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGA                                                                                >  SRR4302542.3294191/1‑94 (MQ=60)
                CCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAA                                                                               <  SRR4302542.5122875/94‑1 (MQ=60)
                CCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAA                                                                               >  SRR4302542.3341119/1‑94 (MQ=60)
                 CCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAG                                                                              <  SRR4302542.4120411/94‑1 (MQ=60)
                 CCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAG                                                                              <  SRR4302542.6864030/94‑1 (MQ=60)
                 CCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAG                                                                              <  SRR4302542.5772730/94‑1 (MQ=60)
                  CATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGA                                                                             <  SRR4302542.3326350/94‑1 (MQ=60)
                   ATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAG                                                                            <  SRR4302542.3899471/94‑1 (MQ=60)
                   ATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAG                                                                            >  SRR4302542.4200986/1‑94 (MQ=60)
                   ATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAG                                                                            <  SRR4302542.4383602/94‑1 (MQ=60)
                   ATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAG                                                                            <  SRR4302542.276795/94‑1 (MQ=60)
                   ATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAG                                                                            <  SRR4302542.4099609/94‑1 (MQ=60)
                    TCCAAGAGCACGCTTATTTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCCCTGATCAAAGACGAAGAG                                                                            <  SRR4302542.2394743/94‑1 (MQ=60)
                    TCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGG                                                                           >  SRR4302542.2513051/1‑94 (MQ=60)
                    TCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTAAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGG                                                                           >  SRR4302542.955870/1‑94 (MQ=60)
                     CCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGG                                                                          <  SRR4302542.3642699/94‑1 (MQ=60)
                     CCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGG                                                                          <  SRR4302542.2354170/94‑1 (MQ=60)
                     CCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGG                                                                          >  SRR4302542.6845939/1‑94 (MQ=60)
                     CCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGG                                                                          >  SRR4302542.5943325/1‑94 (MQ=60)
                      CAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGT                                                                         <  SRR4302542.738918/94‑1 (MQ=60)
                      CAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGT                                                                         >  SRR4302542.5592194/1‑94 (MQ=60)
                       AAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACTCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTA                                                                        <  SRR4302542.2475679/94‑1 (MQ=60)
                       AAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTA                                                                        >  SRR4302542.6902369/1‑94 (MQ=60)
                        AGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAA                                                                       >  SRR4302542.3528887/1‑94 (MQ=60)
                        AGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAA                                                                       <  SRR4302542.2082428/94‑1 (MQ=60)
                        AGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGGAA                                                                       >  SRR4302542.1300021/1‑94 (MQ=60)
                         GAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAA                                                                      <  SRR4302542.307498/94‑1 (MQ=60)
                          AGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAG                                                                     <  SRR4302542.4925870/94‑1 (MQ=60)
                          AGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAG                                                                     >  SRR4302542.2768613/1‑94 (MQ=60)
                          AGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAG                                                                     <  SRR4302542.3472121/94‑1 (MQ=60)
                           GCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGA                                                                    <  SRR4302542.3851976/94‑1 (MQ=60)
                            CACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAA                                                                   <  SRR4302542.698470/94‑1 (MQ=60)
                            CACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAA                                                                   >  SRR4302542.6715280/1‑94 (MQ=60)
                            CACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAA                                                                   >  SRR4302542.5610730/1‑94 (MQ=60)
                             ACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAA                                                                  <  SRR4302542.1204958/94‑1 (MQ=60)
                              CGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAAT                                                                 <  SRR4302542.6038029/94‑1 (MQ=60)
                              CGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAAT                                                                 <  SRR4302542.1080014/94‑1 (MQ=60)
                              CGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAAT                                                                 >  SRR4302542.5531745/1‑94 (MQ=60)
                               GCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATG                                                                >  SRR4302542.124866/1‑94 (MQ=60)
                               GCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATG                                                                >  SRR4302542.2773786/1‑94 (MQ=60)
                                CTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGA                                                               <  SRR4302542.4334888/94‑1 (MQ=60)
                                CTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGA                                                               <  SRR4302542.2950051/94‑1 (MQ=60)
                                 TTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGAT                                                              <  SRR4302542.4333993/94‑1 (MQ=60)
                                   A‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCC                                                            <  SRR4302542.2803685/94‑1 (MQ=60)
                                     TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCT                                                           <  SRR4302542.7322758/94‑1 (MQ=60)
                                      TCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTC                                                          <  SRR4302542.3007477/94‑1 (MQ=60)
                                       CACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCT                                                         <  SRR4302542.1059222/94‑1 (MQ=60)
                                         CCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCC                                                       <  SRR4302542.3062744/94‑1 (MQ=60)
                                         CCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCC                                                       <  SRR4302542.3397207/94‑1 (MQ=60)
                                         CCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCC                                                       >  SRR4302542.694367/1‑94 (MQ=60)
                                          CAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCT                                                      <  SRR4302542.3115285/94‑1 (MQ=60)
                                          CAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCT                                                      >  SRR4302542.6340714/1‑94 (MQ=60)
                                          CAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCT                                                      >  SRR4302542.2063875/1‑94 (MQ=60)
                                          CAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCT                                                      <  SRR4302542.6984667/94‑1 (MQ=60)
                                           AGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTA                                                     >  SRR4302542.1398251/1‑94 (MQ=60)
                                           AGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTA                                                     <  SRR4302542.1637740/94‑1 (MQ=60)
                                            GGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTAT                                                    <  SRR4302542.2252537/94‑1 (MQ=60)
                                             GGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATC                                                   >  SRR4302542.1985146/1‑94 (MQ=60)
                                              GTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCT                                                  >  SRR4302542.3566343/1‑94 (MQ=60)
                                              GTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCT                                                  <  SRR4302542.5736117/94‑1 (MQ=60)
                                              GTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCT                                                  <  SRR4302542.6680915/94‑1 (MQ=60)
                                               TGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTG                                                 <  SRR4302542.5794135/94‑1 (MQ=60)
                                                  AAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAAT                                              <  SRR4302542.1671491/94‑1 (MQ=60)
                                                   AAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATC                                             <  SRR4302542.7474066/94‑1 (MQ=60)
                                                   AAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATC                                             <  SRR4302542.4081786/94‑1 (MQ=60)
                                                   AAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATC                                             >  SRR4302542.6768441/1‑94 (MQ=60)
                                                    AAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCA                                            <  SRR4302542.1812592/94‑1 (MQ=60)
                                                    AAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCA                                            <  SRR4302542.1063377/94‑1 (MQ=60)
                                                     AGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAG                                           <  SRR4302542.2879981/94‑1 (MQ=60)
                                                     AGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAG                                           >  SRR4302542.4564921/1‑94 (MQ=60)
                                                      GCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGG                                          <  SRR4302542.2308152/94‑1 (MQ=60)
                                                       CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGG                                         <  SRR4302542.3519562/94‑1 (MQ=60)
                                                       CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGG                                         >  SRR4302542.152074/1‑94 (MQ=60)
                                                       CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGG                                         >  SRR4302542.3336687/1‑94 (MQ=60)
                                                        GGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGT                                        <  SRR4302542.5316501/94‑1 (MQ=60)
                                                        GGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGT                                        <  SRR4302542.6974595/94‑1 (MQ=60)
                                                         GAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTG                                       >  SRR4302542.1468466/1‑94 (MQ=60)
                                                          AAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGA                                      >  SRR4302542.1306867/1‑94 (MQ=60)
                                                           AACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAT                                     <  SRR4302542.3888089/94‑1 (MQ=60)
                                                            ACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATT                                    >  SRR4302542.2432480/1‑94 (MQ=60)
                                                             CGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTT                                   >  SRR4302542.695346/1‑94 (MQ=60)
                                                             CGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTT                                   >  SRR4302542.6711424/1‑94 (MQ=60)
                                                              GCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTT                                  >  SRR4302542.792343/1‑94 (MQ=60)
                                                                TGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAT                                >  SRR4302542.3473139/1‑94 (MQ=60)
                                                                TGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAT                                <  SRR4302542.7334959/94‑1 (MQ=60)
                                                                 GTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATT                               <  SRR4302542.7372885/94‑1 (MQ=60)
                                                                 GTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATT                               >  SRR4302542.7170501/1‑94 (MQ=60)
                                                                  TACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTG                              >  SRR4302542.4604788/1‑94 (MQ=60)
                                                                  TACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTG                              <  SRR4302542.3334734/94‑1 (MQ=60)
                                                                   ACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGG                             >  SRR4302542.6567894/1‑94 (MQ=60)
                                                                   ACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGG                             <  SRR4302542.5520549/94‑1 (MQ=60)
                                                                    CTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGC                            <  SRR4302542.460966/94‑1 (MQ=60)
                                                                    CTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGC                            >  SRR4302542.4113393/1‑94 (MQ=60)
                                                                      ACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGA                          <  SRR4302542.1086875/94‑1 (MQ=60)
                                                                       CATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAA                         >  SRR4302542.533815/1‑94 (MQ=60)
                                                                        ATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAAC                        <  SRR4302542.5368024/94‑1 (MQ=60)
                                                                        ATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAAC                        >  SRR4302542.6150428/1‑94 (MQ=60)
                                                                         TCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACT                       <  SRR4302542.769545/94‑1 (MQ=60)
                                                                         TCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACT                       <  SRR4302542.287882/94‑1 (MQ=60)
                                                                         TCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACT                       <  SRR4302542.4428248/94‑1 (MQ=60)
                                                                         TCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACT                       <  SRR4302542.2130524/94‑1 (MQ=60)
                                                                         TCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACT                       <  SRR4302542.1096661/94‑1 (MQ=60)
                                                                          CGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTG                      >  SRR4302542.7035371/1‑94 (MQ=60)
                                                                          CGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTG                      <  SRR4302542.4885022/94‑1 (MQ=60)
                                                                          CGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTG                      <  SRR4302542.6850752/94‑1 (MQ=60)
                                                                          CGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTG                      >  SRR4302542.5654472/1‑94 (MQ=60)
                                                                          CGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTG                      <  SRR4302542.79944/94‑1 (MQ=60)
                                                                           GTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGG                     >  SRR4302542.5135477/1‑94 (MQ=60)
                                                                           GTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGG                     >  SRR4302542.5830173/1‑94 (MQ=60)
                                                                           GTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGG                     >  SRR4302542.1544568/1‑94 (MQ=60)
                                                                            TTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGG                    <  SRR4302542.2516121/94‑1 (MQ=60)
                                                                            TTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGG                    >  SRR4302542.5605740/1‑94 (MQ=60)
                                                                            TTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGG                    <  SRR4302542.3382267/94‑1 (MQ=60)
                                                                              AAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCC                  <  SRR4302542.7463211/94‑1 (MQ=60)
                                                                              AAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCC                  <  SRR4302542.1264592/94‑1 (MQ=60)
                                                                              AAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCC                  >  SRR4302542.627318/1‑94 (MQ=60)
                                                                              AAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCC                  <  SRR4302542.5137851/94‑1 (MQ=60)
                                                                              AAAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCC                  >  SRR4302542.3790151/1‑94 (MQ=60)
                                                                               AAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT                 <  SRR4302542.1443076/94‑1 (MQ=60)
                                                                               AAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT                 >  SRR4302542.4233820/1‑94 (MQ=60)
                                                                               AAGGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCT                 <  SRR4302542.5271217/94‑1 (MQ=60)
                                                                                AGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG                >  SRR4302542.7162827/1‑94 (MQ=60)
                                                                                 GGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGT               >  SRR4302542.4292050/1‑94 (MQ=60)
                                                                                 GGCTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGT               >  SRR4302542.6679162/1‑94 (MQ=60)
                                                                                   CTCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTT             >  SRR4302542.1270059/1‑94 (MQ=60)
                                                                                    TCTGTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTG            <  SRR4302542.1693345/94‑1 (MQ=60)
                                                                                      TGTGGCAGCACTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAA          <  SRR4302542.4221077/94‑1 (MQ=60)
                                                                                       GTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAG         <  SRR4302542.2859989/94‑1 (MQ=60)
                                                                                       GTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAG         >  SRR4302542.3020647/1‑94 (MQ=60)
                                                                                       GTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAG         <  SRR4302542.3317557/94‑1 (MQ=60)
                                                                                       GTGGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAG         <  SRR4302542.5203006/94‑1 (MQ=60)
                                                                                        TGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGA        <  SRR4302542.2665195/94‑1 (MQ=60)
                                                                                         GGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAG       <  SRR4302542.5822456/94‑1 (MQ=60)
                                                                                         GGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAG       <  SRR4302542.7302707/94‑1 (MQ=60)
                                                                                         GGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAG       >  SRR4302542.4673540/1‑94 (MQ=60)
                                                                                         GGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAG       <  SRR4302542.3853699/94‑1 (MQ=60)
                                                                                         GGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAG       <  SRR4302542.4307284/94‑1 (MQ=60)
                                                                                         GGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAG       <  SRR4302542.7422665/94‑1 (MQ=60)
                                                                                         GGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAG       <  SRR4302542.3216355/94‑1 (MQ=60)
                                                                                         GGCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAG       <  SRR4302542.1499562/94‑1 (MQ=60)
                                                                                          GCAGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGG      <  SRR4302542.4731254/94‑1 (MQ=60)
                                                                                            AGCGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC    >  SRR4302542.2030260/1‑94 (MQ=60)
                                                                                             GTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCC   <  SRR4302542.1361686/94‑1 (MQ=60)
                                                                                              CGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCA  >  SRR4302542.2115670/1‑94 (MQ=60)
                                                                                              CGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCA  >  SRR4302542.2178914/1‑94 (MQ=60)
                                                                                              |                                                                                             
GCCACATTCATAAGTACCCATCCAAGAGCACGCTTA‑TTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCA  >  NC_000913/3483812‑3483998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑