Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,783,092	T→C	V69A (GTT→GCT)	yibO →	putative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,783,092	0	T	C	86.4%	635.3 / 86.9	265	V69A (GTT→GCT)	yibO	putative 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand): ref base T (16/20); new base C (98/131); total (114/151)
Fisher's exact test for biased strand distribution p-value = 8.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-02

Only 100 of 267 total aligned reads displayed.

GTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGA  >  NC_000913/3783000‑3783184

gtgGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGt                                                                                              <  1:4620481/93‑1 (MQ=255)

  gggCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTa                                                                                            <  1:1520803/93‑1 (MQ=255)

  gggCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTa                                                                                            >  1:1923127/1‑93 (MQ=255)

   ggCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTaa                                                                                           <  1:3557705/93‑1 (MQ=255)

   ggCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTaa                                                                                           <  2:212846/93‑1 (MQ=255)

    gCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAAc                                                                                          >  2:1649795/1‑93 (MQ=255)

      cAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCt                                                                                        <  1:6474445/93‑1 (MQ=255)

      cAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCt                                                                                        >  1:5639181/1‑93 (MQ=255)

      cAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCt                                                                                        >  2:1184821/1‑93 (MQ=255)

      cAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCt                                                                                        >  1:3001978/1‑93 (MQ=255)

       aaTCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTg                                                                                       <  2:400649/93‑1 (MQ=255)

       aaTCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTg                                                                                       >  1:1399415/1‑93 (MQ=255)

       aaTCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTg                                                                                       <  1:7273976/93‑1 (MQ=255)

       aaTCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTg                                                                                       >  2:2663838/1‑93 (MQ=255)

        aTCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTgg                                                                                      >  2:60235/1‑93 (MQ=255)

        aTCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTgg                                                                                      >  1:5398593/1‑93 (MQ=255)

        aTCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTgg                                                                                      >  1:4953624/1‑93 (MQ=255)

        aTCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTgg                                                                                      <  2:3225068/93‑1 (MQ=255)

         tCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTggg                                                                                     <  1:5353774/93‑1 (MQ=255)

          cGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGt                                                                                    <  2:5838274/93‑1 (MQ=255)

           gTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTg                                                                                   <  2:1993127/93‑1 (MQ=255)

              cGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTgccg                                                                                >  2:6200225/1‑93 (MQ=255)

                  tACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTgccggccg                                                                            >  1:6010016/1‑93 (MQ=255)

                  tACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTgccggccg                                                                            >  1:3812889/1‑93 (MQ=255)

                  tACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTgccggccg                                                                            >  2:1142942/1‑93 (MQ=255)

                    cccTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCa                                                                          >  1:4544408/1‑93 (MQ=255)

                    cccTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCa                                                                          <  1:6781847/93‑1 (MQ=255)

                    cccTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCa                                                                          >  1:655333/1‑93 (MQ=255)

                       tAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCg                                                                       >  2:3735067/1‑93 (MQ=255)

                       tAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCg                                                                       >  1:2044450/1‑93 (MQ=255)

                       tAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACATTAACCTGGGTGCCGGCCGCATCg                                                                       >  2:4783443/1‑93 (MQ=255)

                        aaTCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCgt                                                                      >  1:138044/1‑93 (MQ=255)

                        aaTCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCgt                                                                      <  2:6881491/93‑1 (MQ=255)

                        aaTCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCgt                                                                      <  1:429248/93‑1 (MQ=255)

                         aTCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCgtg                                                                     <  1:3468319/93‑1 (MQ=255)

                         aTCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCgtg                                                                     <  2:6367112/93‑1 (MQ=255)

                          tCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCgtgt                                                                    >  1:7522273/1‑93 (MQ=255)

                           cGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTa                                                                   >  2:1547021/1‑93 (MQ=255)

                            gACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTAt                                                                  >  2:2436203/1‑93 (MQ=255)

                             aCGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATc                                                                 >  1:1759869/1‑93 (MQ=255)

                             aCGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATc                                                                 <  2:1058619/93‑1 (MQ=255)

                              cGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCa                                                                <  1:2615495/93‑1 (MQ=255)

                               gCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAg                                                               >  2:2231982/1‑93 (MQ=255)

                               gCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAg                                                               <  2:7024127/93‑1 (MQ=255)

                                 ttCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGa                                                             <  2:7129963/93‑1 (MQ=255)

                                 ttCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGa                                                             >  2:6904348/1‑93 (MQ=255)

                                 ttCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGa                                                             >  2:2488860/1‑93 (MQ=255)

                                  tCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAc                                                            >  2:2204211/1‑93 (MQ=255)

                                   ccGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAcc                                                           <  2:202189/93‑1 (MQ=255)

                                   ccGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAcc                                                           <  2:5410028/93‑1 (MQ=255)

                                   ccGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAcc                                                           <  1:5210628/93‑1 (MQ=255)

                                     ggTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTg                                                         <  2:1297069/93‑1 (MQ=255)

                                      gTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCtnn                                                        >  2:1929166/1‑91 (MQ=255)

                                       tCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGAc                                                       <  2:29390/93‑1 (MQ=255)

                                       tCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGAc                                                       <  2:7135254/93‑1 (MQ=255)

                                       tCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGAc                                                       <  2:179073/93‑1 (MQ=255)

                                       tCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGAc                                                       <  1:6233561/93‑1 (MQ=255)

                                       tCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGAc                                                       >  2:4337378/1‑93 (MQ=255)

                                          ggAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCg                                                    >  1:2544012/1‑93 (MQ=255)

                                             aGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCt                                                 >  1:7320931/1‑93 (MQ=255)

                                             aGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCt                                                 <  2:4161328/93‑1 (MQ=255)

                                              gTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTg                                                >  2:2409369/1‑93 (MQ=255)

                                              gTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTg                                                >  1:7128421/1‑93 (MQ=255)

                                                cGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGa                                              <  1:6441552/93‑1 (MQ=255)

                                                cGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGa                                              <  1:3974470/93‑1 (MQ=255)

                                                  gTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACg                                            >  2:6479580/1‑93 (MQ=255)

                                                     tGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTg                                         >  2:3724285/1‑93 (MQ=255)

                                                      gCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGa                                        <  1:5708045/93‑1 (MQ=255)

                                                        cTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGaaa                                      >  1:5018322/1‑93 (MQ=255)

                                                             cGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCaaa                                 <  1:492400/93‑1 (MQ=255)

                                                             cGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCaaa                                 <  1:940279/93‑1 (MQ=255)

                                                              gTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAg                                <  2:3046782/93‑1 (MQ=255)

                                                              gTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAg                                >  2:7391466/1‑93 (MQ=255)

                                                               tCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGa                               <  1:122448/93‑1 (MQ=255)

                                                               tCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGa                               <  2:61841/93‑1 (MQ=255)

                                                               tCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGa                               <  1:1096014/93‑1 (MQ=255)

                                                               tCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGa                               <  1:4073337/93‑1 (MQ=255)

                                                                cAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGAt                              <  1:3300075/93‑1 (MQ=255)

                                                                cAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGAt                              <  2:4609889/93‑1 (MQ=255)

                                                                 aGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATc                             <  1:6816907/93‑1 (MQ=255)

                                                                 aGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATc                             >  1:3174477/1‑93 (MQ=255)

                                                                 aGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATc                             <  2:6423820/93‑1 (MQ=255)

                                                                  gATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATcg                            >  1:259999/1‑93 (MQ=255)

                                                                         aaCTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGctttc                     <  2:1511936/93‑1 (MQ=255)

                                                                         aaCTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGctttc                     <  1:1303861/93‑1 (MQ=255)

                                                                         aaCTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGctttc                     <  2:3132386/93‑1 (MQ=255)

                                                                           cTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGctttctt                   <  2:1621682/93‑1 (MQ=255)

                                                                            tCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGctttcttt                  >  1:2850679/1‑93 (MQ=255)

                                                                            tCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGctttcttt                  >  2:5743846/1‑93 (MQ=255)

                                                                            tCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGctttcttt                  <  2:6711483/93‑1 (MQ=255)

                                                                              cGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGc                <  2:826518/93‑1 (MQ=255)

                                                                               gAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCt               <  1:988816/93‑1 (MQ=255)

                                                                                 aGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTaa             <  1:6331976/93‑1 (MQ=255)

                                                                                  gTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAAt            <  1:2138976/93‑1 (MQ=255)

                                                                                   tAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATc           <  1:5810972/93‑1 (MQ=255)

                                                                                     ggCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCg         <  1:5944418/93‑1 (MQ=255)

                                                                                     ggCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCg         <  1:2288435/93‑1 (MQ=255)

                                                                                         aCGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGc     <  2:5220852/93‑1 (MQ=255)

                                                                                         aCGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGc     >  2:2559060/1‑93 (MQ=255)

                                                                                            ttAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGa  >  2:2335339/1‑93 (MQ=255)

GTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGA  >  NC_000913/3783000‑3783184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGAC  >  NC_000913/3782999‑3783185

TGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGT                                                                                               <  SRR4302542.997896/94‑1 (MQ=60)

 GTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTT                                                                                              <  SRR4302542.4665623/94‑1 (MQ=60)

 GTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCT                                                                                              >  SRR4302542.1190715/1‑94 (MQ=60)

 GTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCT                                                                                              <  SRR4302542.5290826/94‑1 (MQ=60)

 GTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTACCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCT                                                                                              <  SRR4302542.3378948/94‑1 (MQ=60)

  TGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTA                                                                                             >  SRR4302542.1939740/1‑94 (MQ=60)

   GGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAA                                                                                            <  SRR4302542.1533936/94‑1 (MQ=60)

   GGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAA                                                                                            >  SRR4302542.2144739/1‑94 (MQ=60)

   GGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAA                                                                                            <  SRR4302542.2008540/94‑1 (MQ=60)

   GGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAA                                                                                            >  SRR4302542.6509661/1‑94 (MQ=60)

    GGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAAC                                                                                           <  SRR4302542.3590939/94‑1 (MQ=60)

     GCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACC                                                                                          <  SRR4302542.1393753/94‑1 (MQ=60)

     GCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACC                                                                                          <  SRR4302542.5921155/94‑1 (MQ=60)

      CCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCT                                                                                         <  SRR4302542.5710447/94‑1 (MQ=60)

      CCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCT                                                                                         >  SRR4302542.3030689/1‑94 (MQ=60)

      CCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCT                                                                                         >  SRR4302542.5693940/1‑94 (MQ=60)

       CAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTG                                                                                        >  SRR4302542.1411510/1‑94 (MQ=60)

       CAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTG                                                                                        <  SRR4302542.6538231/94‑1 (MQ=60)

       CAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTG                                                                                        >  SRR4302542.676488/1‑94 (MQ=60)

        AATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGG                                                                                       >  SRR4302542.6013055/1‑94 (MQ=60)

        AATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGG                                                                                       >  SRR4302542.5715224/1‑94 (MQ=60)

        AATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGG                                                                                       <  SRR4302542.7345273/94‑1 (MQ=60)

        AATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGG                                                                                       >  SRR4302542.5451032/1‑94 (MQ=60)

        AATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGG                                                                                       <  SRR4302542.3991898/94‑1 (MQ=60)

        AATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGG                                                                                       >  SRR4302542.5001758/1‑94 (MQ=60)

        AATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGG                                                                                       >  SRR4302542.4188835/1‑94 (MQ=60)

        AATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGG                                                                                       >  SRR4302542.4559238/1‑94 (MQ=60)

         ATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGG                                                                                      >  SRR4302542.1582950/1‑94 (MQ=60)

         ATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGG                                                                                      <  SRR4302542.7320742/94‑1 (MQ=60)

          TCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGT                                                                                     <  SRR4302542.5405795/94‑1 (MQ=60)

           CGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTG                                                                                    <  SRR4302542.7482031/94‑1 (MQ=60)

           CGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTG                                                                                    <  SRR4302542.300160/94‑1 (MQ=60)

           CGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTG                                                                                    <  SRR4302542.6580990/94‑1 (MQ=60)

            GTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGC                                                                                   >  SRR4302542.4964734/1‑94 (MQ=60)

              CCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCG                                                                                 >  SRR4302542.6870667/1‑94 (MQ=60)

               CGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGG                                                                                >  SRR4302542.2524597/1‑94 (MQ=60)

                  ATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCG                                                                             >  SRR4302542.6068214/1‑94 (MQ=60)

                  ATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCG                                                                             >  SRR4302542.5176537/1‑94 (MQ=60)

                  ATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCG                                                                             >  SRR4302542.3850609/1‑94 (MQ=60)

                    ACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCA                                                                           >  SRR4302542.660990/1‑94 (MQ=60)

                    ACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCA                                                                           >  SRR4302542.4588889/1‑94 (MQ=60)

                     CCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCAT                                                                          <  SRR4302542.6848557/94‑1 (MQ=60)

                     CCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCAT                                                                          <  SRR4302542.990344/94‑1 (MQ=60)

                     CCATAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCAT                                                                          >  SRR4302542.1842355/1‑94 (MQ=60)

                      CCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATC                                                                         >  SRR4302542.5037815/1‑94 (MQ=60)

                       CTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCG                                                                        >  SRR4302542.2062123/1‑94 (MQ=60)

                        TAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGT                                                                       >  SRR4302542.139195/1‑94 (MQ=60)

                        TAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGT                                                                       >  SRR4302542.4061232/1‑94 (MQ=60)

                        TAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGT                                                                       >  SRR4302542.1979501/1‑94 (MQ=60)

                         AATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTG                                                                      <  SRR4302542.432956/94‑1 (MQ=60)

                          ATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGT                                                                     <  SRR4302542.3500794/94‑1 (MQ=60)

                          ATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGT                                                                     >  SRR4302542.7596244/1‑94 (MQ=60)

                          ATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGT                                                                     <  SRR4302542.5412143/94‑1 (MQ=60)

                          ATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGT                                                                     <  SRR4302542.3245744/94‑1 (MQ=60)

                             GACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATC                                                                  >  SRR4302542.1775138/1‑94 (MQ=60)

                               atctgaCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAG                                                                >  SRR4302542.462717/7‑94 (MQ=60)

                               CGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAG                                                                <  SRR4302542.2640766/94‑1 (MQ=60)

                                  TTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGAC                                                             <  SRR4302542.5905985/94‑1 (MQ=60)

                                   TCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACC                                                            <  SRR4302542.2925967/94‑1 (MQ=60)

                                    CCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCT                                                           <  SRR4302542.5261302/94‑1 (MQ=60)

                                      GGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGA                                                         <  SRR4302542.4997356/94‑1 (MQ=60)

                                      GGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGA                                                         >  SRR4302542.750806/1‑94 (MQ=60)

                                      GGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGA                                                         >  SRR4302542.2872171/1‑94 (MQ=60)

                                       GTCTGGAAGTCGGTCTGCCTGACCGTCAGGTGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGAC                                                        >  SRR4302542.4123948/1‑94 (MQ=60)

                                        TCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACT                                                       <  SRR4302542.6294934/94‑1 (MQ=60)

                                         CTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTC                                                      <  SRR4302542.3576150/94‑1 (MQ=60)

                                         CTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTC                                                      >  SRR4302542.2601404/1‑94 (MQ=60)

                                          TGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCG                                                     >  SRR4302542.2568672/1‑94 (MQ=60)

                                             AAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCT                                                  >  SRR4302542.4987485/1‑94 (MQ=60)

                                             AAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCT                                                  <  SRR4302542.6903130/94‑1 (MQ=60)

                                             AAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCT                                                  <  SRR4302542.3447260/94‑1 (MQ=60)

                                             AAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCT                                                  >  SRR4302542.7392633/1‑94 (MQ=60)

                                              AGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTG                                                 <  SRR4302542.6393581/94‑1 (MQ=60)

                                              AGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTG                                                 >  SRR4302542.1479307/1‑94 (MQ=60)

                                              AGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTG                                                 >  SRR4302542.7198381/1‑94 (MQ=60)

                                               GTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGG                                                >  SRR4302542.527333/1‑94 (MQ=60)

                                                 CGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGAC                                              <  SRR4302542.1608024/94‑1 (MQ=60)

                                                 CGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGAC                                              <  SRR4302542.3807742/94‑1 (MQ=60)

                                                 CGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGAC                                              <  SRR4302542.644152/94‑1 (MQ=60)

                                                 CGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGAC                                              <  SRR4302542.4013684/94‑1 (MQ=60)

                                                 CGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGAC                                              <  SRR4302542.6504992/94‑1 (MQ=60)

                                                    TCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTT                                           <  SRR4302542.2590077/94‑1 (MQ=60)

                                                    TCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTT                                           >  SRR4302542.5334651/1‑94 (MQ=60)

                                                      TGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGA                                         <  SRR4302542.7338927/94‑1 (MQ=60)

                                                       GCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAA                                        <  SRR4302542.237220/94‑1 (MQ=60)

                                                       GCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAA                                        <  SRR4302542.65442/94‑1 (MQ=60)

                                                       GCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAA                                        <  SRR4302542.5763454/94‑1 (MQ=60)

                                                        CCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAA                                       >  SRR4302542.5067056/1‑94 (MQ=60)

                                                         CTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAAT                                      >  SRR4302542.2068843/1‑94 (MQ=60)

                                                         CTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAAT                                      >  SRR4302542.5799609/1‑94 (MQ=60)

                                                         CTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAAT                                      >  SRR4302542.1282669/1‑94 (MQ=60)

                                                         CTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAAT                                      <  SRR4302542.894498/94‑1 (MQ=60)

                                                         CTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAAT                                      >  SRR4302542.2257816/1‑94 (MQ=60)

                                                            ACCGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAA                                   <  SRR4302542.759683/94‑1 (MQ=60)

                                                              CGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAG                                 <  SRR4302542.948371/94‑1 (MQ=60)

                                                              CGTCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAG                                 <  SRR4302542.496647/94‑1 (MQ=60)

                                                               GTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGA                                <  SRR4302542.3737914/94‑1 (MQ=60)

                                                                TCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGAT                               <  SRR4302542.6834937/94‑1 (MQ=60)

                                                                TCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGAT                               <  SRR4302542.123451/94‑1 (MQ=60)

                                                                TCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGAT                               <  SRR4302542.4113498/94‑1 (MQ=60)

                                                                TCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGAT                               <  SRR4302542.6403728/94‑1 (MQ=60)

                                                                TCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGAT                               <  SRR4302542.1105402/94‑1 (MQ=60)

                                                                TCAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGAT                               >  SRR4302542.1511008/1‑94 (MQ=60)

                                                                 CAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATC                              <  SRR4302542.3331208/94‑1 (MQ=60)

                                                                 CAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATC                              >  SRR4302542.3204576/1‑94 (MQ=60)

                                                                 CAGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATC                              <  SRR4302542.4848656/94‑1 (MQ=60)

                                                                  AGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCG                             <  SRR4302542.4460816/94‑1 (MQ=60)

                                                                  AGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCG                             <  SRR4302542.1007649/94‑1 (MQ=60)

                                                                  AGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCG                             <  SRR4302542.6883920/94‑1 (MQ=60)

                                                                  AGATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCG                             >  SRR4302542.262208/1‑94 (MQ=60)

                                                                    ATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCG                           <  SRR4302542.1305438/94‑1 (MQ=60)

                                                                    ATGGGTAACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCG                           <  SRR4302542.3063182/94‑1 (MQ=60)

                                                                          AACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCT                     >  SRR4302542.267469/1‑94 (MQ=60)

                                                                          AACTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCT                     <  SRR4302542.1315087/94‑1 (MQ=60)

                                                                            CTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTT                   <  SRR4302542.4647937/94‑1 (MQ=60)

                                                                            CTCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTT                   >  SRR4302542.2878082/1‑94 (MQ=60)

                                                                             TCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTG                  <  SRR4302542.6143837/94‑1 (MQ=60)

                                                                             TCCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTG                  <  SRR4302542.5518787/94‑1 (MQ=60)

                                                                              CCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGC                 >  SRR4302542.121257/1‑94 (MQ=60)

                                                                              CCGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGC                 <  SRR4302542.5147766/94‑1 (MQ=60)

                                                                               CGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGATCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCT                >  SRR4302542.3899274/1‑94 (MQ=60)

                                                                               CGAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCT                >  SRR4302542.2625637/1‑94 (MQ=60)

                                                                                GAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTA               <  SRR4302542.997332/94‑1 (MQ=60)

                                                                                GAAGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTA               >  SRR4302542.6011604/1‑94 (MQ=60)

                                                                                  AGTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAAT             <  SRR4302542.6394368/94‑1 (MQ=60)

                                                                                   GTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATC            <  SRR4302542.1317230/94‑1 (MQ=60)

                                                                                   GTAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATC            <  SRR4302542.2157432/94‑1 (MQ=60)

                                                                                    TAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCC           <  SRR4302542.6299057/94‑1 (MQ=60)

                                                                                    TAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCC           <  SRR4302542.7360691/94‑1 (MQ=60)

                                                                                    TAGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCC           <  SRR4302542.5867359/94‑1 (MQ=60)

                                                                                     AGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCG          >  SRR4302542.1364557/1‑94 (MQ=60)

                                                                                     AGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCG          <  SRR4302542.3456421/94‑1 (MQ=60)

                                                                                     AGGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCG          >  SRR4302542.3229818/1‑94 (MQ=60)

                                                                                      GGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGG         <  SRR4302542.6001999/94‑1 (MQ=60)

                                                                                      GGCCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGG         <  SRR4302542.2308098/94‑1 (MQ=60)

                                                                                        CCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTG       <  SRR4302542.5564901/94‑1 (MQ=60)

                                                                                        CCACGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTG       >  SRR4302542.7153442/1‑94 (MQ=60)

                                                                                           CGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTG    >  SRR4302542.2523137/1‑94 (MQ=60)

                                                                                           CGCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTG    <  SRR4302542.6661774/94‑1 (MQ=60)

                                                                                            GCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGA   >  SRR4302542.1535275/1‑94 (MQ=60)

                                                                                            GCTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGA   <  SRR4302542.2942438/94‑1 (MQ=60)

                                                                                             CTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGAC  <  SRR4302542.2178648/94‑1 (MQ=60)

                                                                                             CTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGAC  <  SRR4302542.5581138/94‑1 (MQ=60)

TGTGGGCCAATCGTCCGCATACCCTAATCGACGCTTCCGGTCTGGAAGTCGGTCTGCCTGACCGTCAGATGGGTAACTCCGAAGTAGGCCACGTTAACCTGGGTGCCGGCCGCATCGTGTATCAGGACCTGACTCGTCTGGACGTTGAAATCAAAGATCGCGCTTTCTTTGCTAATCCGGTGCTGAC  >  NC_000913/3782999‑3783185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`