Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,099,449 C→T A63V (GCG→GTG)  kdgT → 2‑keto‑3‑deoxy‑D‑gluconate transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,099,4490CT81.5% 538.2 / 101.1 251A63V (GCG→GTG) kdgT2‑keto‑3‑deoxy‑D‑gluconate transport system
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base T (94/109);  total (117/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-05

Only 100 of 252 total aligned reads displayed.

CGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCA  >  NC_000913/4099359‑4099540
                                                                                          |                                                                                           
 gggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTc                                                                                          >  1:3789371/1‑93 (MQ=255)
 gggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTc                                                                                          >  2:4725547/1‑93 (MQ=255)
  ggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCa                                                                                         >  2:1941981/1‑93 (MQ=255)
   gggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCaa                                                                                        >  2:380371/1‑93 (MQ=255)
        gggAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATaaaa                                                                                   <  1:4104110/93‑1 (MQ=255)
        gggAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATaaaa                                                                                   <  2:3379040/93‑1 (MQ=255)
        gggAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATaaaa                                                                                   >  1:3154583/1‑93 (MQ=255)
          gAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAAtt                                                                                 >  2:2864813/1‑93 (MQ=255)
          gAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAAtt                                                                                 >  1:971302/1‑93 (MQ=255)
           aaaTATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTa                                                                                >  1:2906960/1‑93 (MQ=255)
            aaTATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTaa                                                                               <  1:6514934/93‑1 (MQ=255)
            aaTATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTaa                                                                               >  2:2809279/1‑93 (MQ=255)
            aaTATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTaa                                                                               <  1:3486157/93‑1 (MQ=255)
            aaTATTTTGGATAATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTaa                                                                               <  2:7223166/93‑1 (MQ=255)
             atatTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAg                                                                              <  1:3293006/93‑1 (MQ=255)
              tatTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAgc                                                                             <  2:5237002/93‑1 (MQ=255)
               atTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAgcg                                                                            <  2:427568/93‑1 (MQ=255)
               atTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAgcg                                                                            >  2:149421/1‑93 (MQ=255)
                ttttGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAgcgc                                                                           >  2:6408774/1‑93 (MQ=255)
                 tttGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGcgcg                                                                          <  2:6792535/93‑1 (MQ=255)
                  ttGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGa                                                                         >  2:3022610/1‑93 (MQ=255)
                   tGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGAc                                                                        <  2:3972892/93‑1 (MQ=255)
                    ggATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACg                                                                       >  1:5637551/1‑93 (MQ=255)
                     gATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACgg                                                                      <  1:91676/93‑1 (MQ=255)
                        cATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGaa                                                                   >  2:842002/1‑93 (MQ=255)
                          ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACg                                                                 >  2:5395711/1‑93 (MQ=255)
                          ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACg                                                                 <  1:7361112/93‑1 (MQ=255)
                           tCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACgg                                                                <  2:1005157/93‑1 (MQ=255)
                              ccAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTAc                                                             <  1:6004591/93‑1 (MQ=255)
                                aaCGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTg                                                           >  1:5784539/1‑93 (MQ=255)
                                 aCGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGc                                                          >  2:5466440/1‑93 (MQ=255)
                                 aCGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGc                                                          <  1:7276303/93‑1 (MQ=255)
                                    ggATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTa                                                       >  2:2682004/1‑93 (MQ=255)
                                    ggATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTa                                                       >  1:4987362/1‑93 (MQ=255)
                                     gatgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTaa                                                      >  1:4864785/1‑93 (MQ=255)
                                     gatgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTaa                                                      >  1:2876331/1‑93 (MQ=255)
                                      atgatTACCGGTACGTTGCCCATTCTGGCGTTGTGGTTTTTTGGCATGGGGGTGTAAAAAAAATTAAGCGCGACGGGAACGGTACTGCGTaaa                                                     <  2:1873767/93‑1 (MQ=255)
                                      atgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTaaa                                                     <  1:6203148/93‑1 (MQ=255)
                                      atgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTaaa                                                     <  2:6835281/93‑1 (MQ=255)
                                       tgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAt                                                    <  2:1225751/93‑1 (MQ=255)
                                       tgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAt                                                    >  1:4499073/1‑93 (MQ=255)
                                       tgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAt                                                    >  1:6895920/1‑93 (MQ=255)
                                       tgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAt                                                    >  1:6955988/1‑93 (MQ=255)
                                        gatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATc                                                   <  2:6286799/93‑1 (MQ=255)
                                        gatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATc                                                   <  1:6025875/93‑1 (MQ=255)
                                        gatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATc                                                   <  2:3342023/93‑1 (MQ=255)
                                         atTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATcc                                                  <  1:7460144/93‑1 (MQ=255)
                                         atTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATcc                                                  <  2:6067120/93‑1 (MQ=255)
                                          ttACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCg                                                 <  2:1046526/93‑1 (MQ=255)
                                           tACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCgg                                                <  2:5756950/93‑1 (MQ=255)
                                            aCCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGt                                               >  2:5016069/1‑93 (MQ=255)
                                             ccGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTa                                              <  1:3977503/93‑1 (MQ=255)
                                             ccGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTa                                              <  2:6489448/93‑1 (MQ=255)
                                               ggTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACg                                            >  1:6587737/1‑93 (MQ=255)
                                                gTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGc                                           <  2:350291/93‑1 (MQ=255)
                                                gTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGc                                           >  2:1353532/1‑93 (MQ=255)
                                                gTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGc                                           >  1:7092128/1‑93 (MQ=255)
                                                  aCGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCtg                                         <  1:20786/93‑1 (MQ=255)
                                                     gTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCtggtg                                      >  2:3610521/1‑93 (MQ=255)
                                                         ccATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTaa                                  <  1:4747741/93‑1 (MQ=255)
                                                          cATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAAc                                 <  1:107634/93‑1 (MQ=255)
                                                           aTTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACt                                >  1:5396710/1‑93 (MQ=255)
                                                            ttCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTa                               >  2:5020765/1‑93 (MQ=255)
                                                            ttCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTa                               >  2:7359107/1‑93 (MQ=255)
                                                            ttCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTa                               >  1:4650829/1‑93 (MQ=255)
                                                            ttCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTa                               >  2:4582225/1‑93 (MQ=255)
                                                               tGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTaaaa                            >  2:4113084/1‑93 (MQ=255)
                                                                ggCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAAt                           <  1:1157199/93‑1 (MQ=255)
                                                                ggCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAAt                           <  1:2424586/93‑1 (MQ=255)
                                                                 gCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAAtt                          <  1:6274974/93‑1 (MQ=255)
                                                                 gCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAAtt                          >  2:2134152/1‑93 (MQ=255)
                                                                    gtgtGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGcc                       >  1:1608988/1‑93 (MQ=255)
                                                                    gtgtGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGcc                       >  1:3169102/1‑93 (MQ=255)
                                                                    gtgtGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGcc                       >  2:1370252/1‑93 (MQ=255)
                                                                     tgtgGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCg                      <  2:2268987/93‑1 (MQ=255)
                                                                     tgtgGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCg                      >  1:6785241/1‑93 (MQ=255)
                                                                     cgtgGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCg                      >  1:7201166/2‑93 (MQ=255)
                                                                      gtgGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGt                     <  1:5056001/93‑1 (MQ=255)
                                                                      gtgGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGt                     >  1:4672873/1‑93 (MQ=255)
                                                                         gTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                  >  1:5824953/1‑93 (MQ=255)
                                                                         gTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                  >  1:1683797/1‑93 (MQ=255)
                                                                          tttttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgcg                 >  2:1147959/1‑93 (MQ=255)
                                                                           ttttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgt                >  1:3629268/1‑93 (MQ=255)
                                                                           ttttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgt                <  2:1623798/93‑1 (MQ=255)
                                                                           ttttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgt                <  2:3053401/93‑1 (MQ=255)
                                                                           ttttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgt                <  1:5659060/93‑1 (MQ=255)
                                                                           ttttttGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgt                >  1:1768120/1‑93 (MQ=255)
                                                                            tttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGTGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtg               <  1:4548310/93‑1 (MQ=255)
                                                                            tttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtg               >  2:3680435/1‑93 (MQ=255)
                                                                             ttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgg              <  1:1487872/93‑1 (MQ=255)
                                                                             ttttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgg              <  1:4239936/93‑1 (MQ=255)
                                                                              tttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtggg             <  1:2313983/93‑1 (MQ=255)
                                                                               ttGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgggt            >  1:4199496/1‑93 (MQ=255)
                                                                                  cATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGggtggt         <  1:6424954/93‑1 (MQ=255)
                                                                                    tGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTg       >  2:1717739/1‑93 (MQ=255)
                                                                                    tGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTg       <  2:5213516/93‑1 (MQ=255)
                                                                                     gggggCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGc      >  1:5230268/1‑93 (MQ=255)
                                                                                       gggTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCgg    >  1:4352354/1‑93 (MQ=255)
                                                                                       gggCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCgg    >  2:7085678/1‑93 (MQ=255)
                                                                                         gCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCa  <  1:2223582/93‑1 (MQ=255)
                                                                                          |                                                                                           
CGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCA  >  NC_000913/4099359‑4099540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAT  >  NC_000913/4099356‑4099542
                                                                                             |                                                                                             
CGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGT                                                                                               <  SRR4302542.878519/94‑1 (MQ=60)
 GCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTG                                                                                              <  SRR4302542.5415217/94‑1 (MQ=60)
   CGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTC                                                                                            >  SRR4302542.3826837/1‑94 (MQ=60)
    GGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCA                                                                                           >  SRR4302542.1880937/1‑94 (MQ=60)
    GGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCA                                                                                           <  SRR4302542.6834846/94‑1 (MQ=60)
       GGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATA                                                                                        <  SRR4302542.1093879/94‑1 (MQ=60)
       GGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATA                                                                                        >  SRR4302542.5296550/1‑94 (MQ=60)
         CGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAA                                                                                      >  SRR4302542.6176002/1‑94 (MQ=60)
          GGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAA                                                                                     >  SRR4302542.3184535/1‑94 (MQ=60)
          GGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAA                                                                                     >  SRR4302542.88669/1‑94 (MQ=60)
          GGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAA                                                                                     <  SRR4302542.2113534/94‑1 (MQ=60)
           GGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAAT                                                                                    <  SRR4302542.6922436/94‑1 (MQ=60)
           GGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAAT                                                                                    <  SRR4302542.4144572/94‑1 (MQ=60)
           GGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAAT                                                                                    >  SRR4302542.4556690/1‑94 (MQ=60)
            GGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATT                                                                                   <  SRR4302542.6138130/94‑1 (MQ=60)
            GGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATT                                                                                   >  SRR4302542.7279837/1‑94 (MQ=60)
            GGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATT                                                                                   >  SRR4302542.979669/1‑94 (MQ=60)
            GGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATT                                                                                   <  SRR4302542.2268061/94‑1 (MQ=60)
            GGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTATGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATT                                                                                   <  SRR4302542.6280642/94‑1 (MQ=60)
             GAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTA                                                                                  >  SRR4302542.2934845/1‑94 (MQ=60)
               AATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAG                                                                                <  SRR4302542.4588439/94‑1 (MQ=60)
               AATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAG                                                                                <  SRR4302542.6579139/94‑1 (MQ=60)
               AATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAG                                                                                <  SRR4302542.3518766/94‑1 (MQ=60)
                ATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGC                                                                               >  SRR4302542.4813749/1‑94 (MQ=60)
                ATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGC                                                                               <  SRR4302542.3324084/94‑1 (MQ=60)
                ATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGC                                                                               <  SRR4302542.7273341/94‑1 (MQ=60)
                  ATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGC                                                                             <  SRR4302542.3195628/94‑1 (MQ=60)
                  ATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGC                                                                             >  SRR4302542.4047146/1‑94 (MQ=60)
                   TTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCG                                                                            <  SRR4302542.4760260/94‑1 (MQ=60)
                   TTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCG                                                                            <  SRR4302542.4984069/94‑1 (MQ=60)
                   TTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCG                                                                            <  SRR4302542.5579590/94‑1 (MQ=60)
                   TTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCG                                                                            <  SRR4302542.7442113/94‑1 (MQ=60)
                    TTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGA                                                                           <  SRR4302542.6607849/94‑1 (MQ=60)
                      TGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACG                                                                         <  SRR4302542.4235988/94‑1 (MQ=60)
                      TGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACG                                                                         >  SRR4302542.3869964/1‑94 (MQ=60)
                      TGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACG                                                                         >  SRR4302542.5692298/1‑94 (MQ=60)
                       GGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGG                                                                        <  SRR4302542.4746558/94‑1 (MQ=60)
                        GATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGG                                                                       <  SRR4302542.92446/94‑1 (MQ=60)
                        GATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGG                                                                       <  SRR4302542.2375522/94‑1 (MQ=60)
                         ATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGA                                                                      >  SRR4302542.5637904/1‑94 (MQ=60)
                           CATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAAC                                                                    <  SRR4302542.5217414/94‑1 (MQ=60)
                           CATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAAC                                                                    >  SRR4302542.3149595/1‑94 (MQ=60)
                           CATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAAC                                                                    <  SRR4302542.3906664/94‑1 (MQ=60)
                           CATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGGGTCAATAAAATTAAGCGCGACGGGAAC                                                                    >  SRR4302542.6063314/1‑94 (MQ=60)
                             ttgaattccaccatttctctgttcaatgattttacTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGG                                                                  <  SRR4302542.6693441/59‑1 (MQ=60)
                             TTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGG                                                                  <  SRR4302542.7433211/94‑1 (MQ=60)
                             TTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGG                                                                  <  SRR4302542.5698849/94‑1 (MQ=60)
                              TCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGT                                                                 <  SRR4302542.245223/94‑1 (MQ=60)
                               CACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTA                                                                <  SRR4302542.4312561/94‑1 (MQ=60)
                                ACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTAC                                                               >  SRR4302542.1477429/1‑94 (MQ=60)
                                 CCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACT                                                              <  SRR4302542.2474854/94‑1 (MQ=60)
                                 CCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACT                                                              <  SRR4302542.1785865/94‑1 (MQ=60)
                                 CCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACT                                                              <  SRR4302542.6062741/94‑1 (MQ=60)
                                 CCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACT                                                              >  SRR4302542.1229397/1‑94 (MQ=60)
                                 CCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACT                                                              <  SRR4302542.1730793/94‑1 (MQ=60)
                                  CAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTG                                                             >  SRR4302542.5840706/1‑94 (MQ=60)
                                   AACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGC                                                            >  SRR4302542.996353/1‑94 (MQ=60)
                                    ACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCG                                                           <  SRR4302542.7347667/94‑1 (MQ=60)
                                    ACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGGGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCG                                                           >  SRR4302542.6610187/1‑94 (MQ=60)
                                      GGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTA                                                         >  SRR4302542.5186336/1‑94 (MQ=60)
                                      GGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTA                                                         >  SRR4302542.5035757/1‑94 (MQ=60)
                                       GGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAA                                                        >  SRR4302542.2903947/1‑94 (MQ=60)
                                       GGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAA                                                        >  SRR4302542.4912151/1‑94 (MQ=60)
                                         ATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAT                                                      >  SRR4302542.4543203/1‑94 (MQ=60)
                                         ATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAT                                                      >  SRR4302542.6963638/1‑94 (MQ=60)
                                         ATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAT                                                      >  SRR4302542.635197/1‑94 (MQ=60)
                                         ATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAT                                                      <  SRR4302542.6263953/94‑1 (MQ=60)
                                         ATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAAT                                                      >  SRR4302542.7024331/1‑94 (MQ=60)
                                          TGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATC                                                     <  SRR4302542.3265230/94‑1 (MQ=60)
                                           GATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCC                                                    <  SRR4302542.2262324/94‑1 (MQ=60)
                                           GATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCC                                                    <  SRR4302542.5062241/94‑1 (MQ=60)
                                           GATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCC                                                    <  SRR4302542.6084229/94‑1 (MQ=60)
                                            ATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCG                                                   <  SRR4302542.7533223/94‑1 (MQ=60)
                                            ATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCG                                                   <  SRR4302542.5006779/94‑1 (MQ=60)
                                             TTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGG                                                  <  SRR4302542.4503755/94‑1 (MQ=60)
                                              TACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGT                                                 <  SRR4302542.6061744/94‑1 (MQ=60)
                                              TACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGT                                                 <  SRR4302542.6425985/94‑1 (MQ=60)
                                               ACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTA                                                >  SRR4302542.5806516/1‑94 (MQ=60)
                                                CCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTAC                                               <  SRR4302542.4016747/94‑1 (MQ=60)
                                                 CGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACG                                              >  SRR4302542.6652643/1‑94 (MQ=60)
                                                  GGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGC                                             >  SRR4302542.7161703/1‑94 (MQ=60)
                                                   GTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCT                                            <  SRR4302542.6556504/94‑1 (MQ=60)
                                                     ACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGG                                          <  SRR4302542.20964/94‑1 (MQ=60)
                                                       GGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTG                                        <  SRR4302542.3104334/94‑1 (MQ=60)
                                                        GTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGAAACGGTACTGCGTAAATCCGGTACGCTGGTGG                                       <  SRR4302542.6981781/94‑1 (MQ=60)
                                                          GCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTA                                     >  SRR4302542.1683626/1‑94 (MQ=60)
                                                            CCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAAC                                   >  SRR4302542.4602398/1‑94 (MQ=60)
                                                            CCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAAC                                   <  SRR4302542.4794012/94‑1 (MQ=60)
                                                             CATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACT                                  <  SRR4302542.2926171/94‑1 (MQ=60)
                                                             CATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACT                                  >  SRR4302542.7218428/1‑94 (MQ=60)
                                                             CATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACT                                  <  SRR4302542.108517/94‑1 (MQ=60)
                                                             CATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACT                                  >  SRR4302542.5449127/1‑94 (MQ=60)
                                                              ATTCTGGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTA                                 >  SRR4302542.377202/1‑94 (MQ=60)
                                                              ATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTA                                 >  SRR4302542.4696293/1‑94 (MQ=60)
                                                                  TGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAAT                             <  SRR4302542.6886010/94‑1 (MQ=60)
                                                                   GGCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATT                            <  SRR4302542.1167117/94‑1 (MQ=60)
                                                                   GGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATT                            <  SRR4302542.2448170/94‑1 (MQ=60)
                                                                    GCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTG                           <  SRR4302542.7405384/94‑1 (MQ=60)
                                                                    GCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTG                           >  SRR4302542.596503/1‑94 (MQ=60)
                                                                    GCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTG                           >  SRR4302542.7031346/1‑94 (MQ=60)
                                                                    GCGGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTG                           <  SRR4302542.6336822/94‑1 (MQ=60)
                                                                      GGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCC                         >  SRR4302542.1622885/1‑94 (MQ=60)
                                                                      GGTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCC                         >  SRR4302542.3199163/1‑94 (MQ=60)
                                                                       GTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCG                        <  SRR4302542.3577464/94‑1 (MQ=60)
                                                                       GTGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCG                        >  SRR4302542.6851977/1‑94 (MQ=60)
                                                                       GCGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCG                        >  SRR4302542.7271837/1‑94 (MQ=60)
                                                                        TGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGT                       <  SRR4302542.4613269/94‑1 (MQ=60)
                                                                        TGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGT                       >  SRR4302542.4718534/1‑94 (MQ=60)
                                                                        TGTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGT                       >  SRR4302542.5793918/1‑94 (MQ=60)
                                                                        TGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGT                       <  SRR4302542.4268779/94‑1 (MQ=60)
                                                                         GTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTC                      >  SRR4302542.1576127/1‑94 (MQ=60)
                                                                         GTGGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTC                      <  SRR4302542.5105099/94‑1 (MQ=60)
                                                                           GGTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGC                    >  SRR4302542.5881494/1‑94 (MQ=60)
                                                                           GGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGC                    >  SRR4302542.1698369/1‑94 (MQ=60)
                                                                            GTTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCG                   >  SRR4302542.901088/1‑94 (MQ=60)
                                                                             TTTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGT                  >  SRR4302542.3663080/1‑94 (MQ=60)
                                                                             TTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGT                  <  SRR4302542.5868058/94‑1 (MQ=60)
                                                                             TTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGT                  >  SRR4302542.1783452/1‑94 (MQ=60)
                                                                              TTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTG                 <  SRR4302542.5714040/94‑1 (MQ=60)
                                                                              TTTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTG                 <  SRR4302542.3632952/94‑1 (MQ=60)
                                                                               TTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGTGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGG                <  SRR4302542.4592825/94‑1 (MQ=60)
                                                                               TTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGG                >  SRR4302542.4698979/1‑94 (MQ=60)
                                                                               TTTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGG                <  SRR4302542.7385293/94‑1 (MQ=60)
                                                                                TTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGG               <  SRR4302542.4281730/94‑1 (MQ=60)
                                                                                TTTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGG               <  SRR4302542.1500741/94‑1 (MQ=60)
                                                                                 TTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGT              <  SRR4302542.2333844/94‑1 (MQ=60)
                                                                                 TTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGT              >  SRR4302542.702545/1‑94 (MQ=60)
                                                                                 TTTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGT              >  SRR4302542.4240901/1‑94 (MQ=60)
                                                                                  TTGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTG             <  SRR4302542.4181115/94‑1 (MQ=60)
                                                                                   TGCATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGG            >  SRR4302542.3472966/1‑94 (MQ=60)
                                                                                     CATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTT          <  SRR4302542.6488269/94‑1 (MQ=60)
                                                                                     CATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTT          <  SRR4302542.3234816/94‑1 (MQ=60)
                                                                                     CATGGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTT          <  SRR4302542.4362244/94‑1 (MQ=60)
                                                                                      ATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTG         <  SRR4302542.925583/94‑1 (MQ=60)
                                                                                       TGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGC        >  SRR4302542.5281131/1‑94 (MQ=60)
                                                                                        GGGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCG       <  SRR4302542.1697635/94‑1 (MQ=60)
                                                                                         GGGGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGG      >  SRR4302542.4395117/1‑94 (MQ=60)
                                                                                           GGTGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCA    <  SRR4302542.6056119/94‑1 (MQ=60)
                                                                                            GCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAA   <  SRR4302542.2242718/94‑1 (MQ=60)
                                                                                             TGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAT  >  SRR4302542.6834413/1‑94 (MQ=60)
                                                                                             CGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAT  >  SRR4302542.2136776/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAT  >  NC_000913/4099356‑4099542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑