Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,133,793 A→G I36V (ATC→GTC)  b3943 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,133,7930AG85.9% 705.7 / 104.2 297I36V (ATC→GTC) b3943hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/19);  new base G (116/139);  total (139/158)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

Only 100 of 298 total aligned reads displayed.

AAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCG  >  NC_000913/4133703‑4133885
                                                                                          |                                                                                            
aaGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTc                                                                                            <  2:4563607/93‑1 (MQ=255)
aaGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTc                                                                                            >  1:439471/1‑93 (MQ=255)
aaGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATc                                                                                            <  1:3330285/93‑1 (MQ=255)
 agagCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCg                                                                                           >  1:4796353/1‑93 (MQ=255)
  gagCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCgg                                                                                          <  1:4178831/93‑1 (MQ=255)
     cAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGc                                                                                       <  1:2044042/93‑1 (MQ=255)
      aaCCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGcc                                                                                      >  1:2563330/1‑93 (MQ=255)
      aaCCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGcc                                                                                      >  2:1131958/1‑93 (MQ=255)
       aCCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCt                                                                                     <  1:3889179/93‑1 (MQ=255)
       aCCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCt                                                                                     >  2:2339592/1‑93 (MQ=255)
       aCCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCt                                                                                     <  1:7073735/93‑1 (MQ=255)
        cccACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCtt                                                                                    >  1:1033624/1‑93 (MQ=255)
         ccACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTc                                                                                   <  1:972241/93‑1 (MQ=255)
         ccACTGGCAATCAGTGGCCTGGTCGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTc                                                                                   >  1:167520/1‑93 (MQ=255)
          cACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCg                                                                                  <  1:598586/93‑1 (MQ=255)
          cACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCg                                                                                  <  1:5458231/93‑1 (MQ=255)
          cACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCg                                                                                  >  1:4138379/1‑93 (MQ=255)
           aCTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGa                                                                                 >  2:548561/1‑93 (MQ=255)
            cTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGAc                                                                                <  1:6797372/93‑1 (MQ=255)
            cTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGAc                                                                                <  1:1073016/93‑1 (MQ=255)
               gCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACttt                                                                             >  2:4408431/1‑93 (MQ=255)
                  aTCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTAcc                                                                          <  1:2281493/93‑1 (MQ=255)
                  aTCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTAcc                                                                          >  1:775321/1‑93 (MQ=255)
                   tCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCg                                                                         >  2:1522445/1‑93 (MQ=255)
                    cAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGc                                                                        >  2:1689713/1‑93 (MQ=255)
                    cAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGc                                                                        <  2:1013159/93‑1 (MQ=255)
                     aGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGcc                                                                       <  1:6482100/93‑1 (MQ=255)
                      gTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCt                                                                      <  2:2838319/93‑1 (MQ=255)
                         gCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTAc                                                                   >  1:6882521/1‑93 (MQ=255)
                         gCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTAc                                                                   <  1:5374747/93‑1 (MQ=255)
                           cTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGc                                                                 <  2:3725045/93‑1 (MQ=255)
                            tGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCt                                                                <  2:4017661/93‑1 (MQ=255)
                              gTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGc                                                              <  2:6851646/93‑1 (MQ=255)
                              gTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGc                                                              >  2:4145642/1‑93 (MQ=255)
                               ttGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCa                                                             <  1:6266143/93‑1 (MQ=255)
                                tgtgCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCAt                                                            >  2:7228257/1‑93 (MQ=255)
                                 gtgCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCAtt                                                           <  2:908105/93‑1 (MQ=255)
                                 gtgCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCAtt                                                           <  1:3632105/93‑1 (MQ=255)
                                  tgCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCAttt                                                          >  1:2164450/1‑93 (MQ=255)
                                   gCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCAtttt                                                         >  2:6166372/1‑93 (MQ=255)
                                   gCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCAtttt                                                         >  2:2104000/1‑93 (MQ=255)
                                    cTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTc                                                        >  1:1598122/1‑93 (MQ=255)
                                    cTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTc                                                        <  2:5944221/93‑1 (MQ=255)
                                     tCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCg                                                       <  1:143768/93‑1 (MQ=255)
                                     tCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCg                                                       >  2:306456/1‑93 (MQ=255)
                                     tCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCg                                                       <  1:5025006/93‑1 (MQ=255)
                                        cacTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGgcg                                                    >  1:4574428/1‑93 (MQ=255)
                                          cTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGct                                                  >  1:5287420/1‑93 (MQ=255)
                                           ttATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGctc                                                 <  2:1453275/93‑1 (MQ=255)
                                           ttATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGctc                                                 >  2:6753904/1‑93 (MQ=255)
                                           ttATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGctc                                                 <  1:1849216/93‑1 (MQ=255)
                                             aTCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCtctc                                               <  2:4772449/93‑1 (MQ=255)
                                              tCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCg                                              >  2:6594123/1‑93 (MQ=255)
                                               cTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGt                                             <  2:4333133/93‑1 (MQ=255)
                                                 ggAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGttt                                           >  1:398870/1‑93 (MQ=255)
                                                   aGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTa                                         <  2:2484945/93‑1 (MQ=255)
                                                    gTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTAt                                        <  2:3275770/93‑1 (MQ=255)
                                                     ttATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTAtt                                       >  1:3092639/1‑93 (MQ=255)
                                                     ttATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTAtt                                       <  1:2664076/93‑1 (MQ=255)
                                                       ataGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCa                                     <  1:1907627/93‑1 (MQ=255)
                                                         aGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATc                                   <  2:5285842/93‑1 (MQ=255)
                                                             ggATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTcc                               <  2:6277713/93‑1 (MQ=255)
                                                              gATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCt                              >  2:3612207/1‑93 (MQ=255)
                                                               aTTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCtt                             >  2:1265591/1‑93 (MQ=255)
                                                               aTTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCtt                             <  1:5211703/93‑1 (MQ=255)
                                                                ttttCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCttt                            <  2:5354633/93‑1 (MQ=255)
                                                                ttttCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCttt                            <  2:7528416/93‑1 (MQ=255)
                                                                  ttCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTtta                          <  2:262938/93‑1 (MQ=255)
                                                                  ttCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTtta                          <  1:5584926/93‑1 (MQ=255)
                                                                  ttCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTtta                          >  1:5908423/1‑93 (MQ=255)
                                                                  tcaaTGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTtta                          <  1:2416249/90‑1 (MQ=255)
                                                                   tCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTttat                         >  2:7162986/1‑93 (MQ=255)
                                                                    cATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTttatt                        >  2:6461314/1‑93 (MQ=255)
                                                                     aTGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTttatta                       >  1:3188425/1‑93 (MQ=255)
                                                                     aTGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTttatta                       <  2:783635/93‑1 (MQ=255)
                                                                     aTGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTttatta                       >  2:4196753/1‑93 (MQ=255)
                                                                      tGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTAc                      <  1:4310655/93‑1 (MQ=255)
                                                                      tGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTAc                      <  1:5724618/93‑1 (MQ=255)
                                                                      tGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTAc                      <  2:7032127/93‑1 (MQ=255)
                                                                      tGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTAc                      >  2:3911092/1‑93 (MQ=255)
                                                                       gAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACg                     <  2:5403901/93‑1 (MQ=255)
                                                                       gAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACg                     >  2:7341918/1‑93 (MQ=255)
                                                                       gAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACg                     >  2:1816056/1‑93 (MQ=255)
                                                                        aaGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGt                    <  1:3256358/93‑1 (MQ=255)
                                                                          gCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTgg                  >  2:2937169/1‑93 (MQ=255)
                                                                          gCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTgg                  >  2:3360544/1‑93 (MQ=255)
                                                                           cAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGt                 <  1:2984225/93‑1 (MQ=255)
                                                                           cAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGt                 <  2:5859902/93‑1 (MQ=255)
                                                                            aaGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTc                <  1:6124353/93‑1 (MQ=255)
                                                                            aaGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTc                <  1:3425906/93‑1 (MQ=255)
                                                                            aaGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTc                <  1:6834526/93‑1 (MQ=255)
                                                                             aGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGtag               >  2:6547762/1‑91 (MQ=255)
                                                                              gTCACCAGTTACGACGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTcgc              <  2:5793362/93‑1 (MQ=255)
                                                                                  ccAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATCACGTGGTCGCGGaa          <  1:7492386/93‑1 (MQ=255)
                                                                                    aGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATg        <  1:2829729/93‑1 (MQ=255)
                                                                                    aGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATg        >  2:3408614/1‑93 (MQ=255)
                                                                                    aGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATg        <  2:3525399/93‑1 (MQ=255)
                                                                                     gTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATgc       <  1:3766156/93‑1 (MQ=255)
                                                                                     gTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATgc       <  1:1323345/93‑1 (MQ=255)
                                                                                     gTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATgc       >  1:6625874/1‑93 (MQ=255)
                                                                                          |                                                                                            
AAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCG  >  NC_000913/4133703‑4133885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGA  >  NC_000913/4133701‑4133886
                                                                                            |                                                                                             
GAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGT                                                                                              >  SRR4302542.3232206/1‑94 (MQ=60)
 AAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTC                                                                                             >  SRR4302542.443282/1‑94 (MQ=60)
 AAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTC                                                                                             <  SRR4302542.1426407/94‑1 (MQ=60)
 AAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTC                                                                                             <  SRR4302542.3033253/94‑1 (MQ=60)
 AAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTC                                                                                             >  SRR4302542.6140634/1‑94 (MQ=60)
  AAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCG                                                                                            >  SRR4302542.4843086/1‑94 (MQ=60)
  AAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCG                                                                                            >  SRR4302542.3981517/1‑94 (MQ=60)
  AAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCG                                                                                            <  SRR4302542.2056079/94‑1 (MQ=60)
  AAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCG                                                                                            <  SRR4302542.3361628/94‑1 (MQ=60)
   AGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGG                                                                                           >  SRR4302542.6905293/1‑94 (MQ=60)
   AGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGG                                                                                           <  SRR4302542.325692/94‑1 (MQ=60)
    GAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGT                                                                                          <  SRR4302542.4220039/94‑1 (MQ=60)
     AGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTG                                                                                         >  SRR4302542.3692425/1‑94 (MQ=60)
       CAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCC                                                                                       >  SRR4302542.2588181/1‑94 (MQ=60)
       CAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCC                                                                                       <  SRR4302542.2061714/94‑1 (MQ=60)
       CAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCC                                                                                       >  SRR4302542.1877379/1‑94 (MQ=60)
        AACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCT                                                                                      <  SRR4302542.6636672/94‑1 (MQ=60)
        AACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCT                                                                                      >  SRR4302542.5356580/1‑94 (MQ=60)
        AACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCT                                                                                      <  SRR4302542.3422350/94‑1 (MQ=60)
         ACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTT                                                                                     <  SRR4302542.3927574/94‑1 (MQ=60)
         ACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTT                                                                                     >  SRR4302542.1042511/1‑94 (MQ=60)
         ACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTT                                                                                     <  SRR4302542.7143157/94‑1 (MQ=60)
         ACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTT                                                                                     <  SRR4302542.557533/94‑1 (MQ=60)
          CCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTC                                                                                    <  SRR4302542.3772014/94‑1 (MQ=60)
          CCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTC                                                                                    >  SRR4302542.3222845/1‑94 (MQ=60)
          CCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTC                                                                                    <  SRR4302542.1892324/94‑1 (MQ=60)
          CCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTC                                                                                    <  SRR4302542.5867248/94‑1 (MQ=60)
          CCCACTGGCAATCAGTGGCCTGGTCGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTC                                                                                    >  SRR4302542.168939/1‑94 (MQ=60)
           CCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCG                                                                                   <  SRR4302542.980624/94‑1 (MQ=60)
           CCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCG                                                                                   >  SRR4302542.4179154/1‑94 (MQ=60)
           CCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCG                                                                                   <  SRR4302542.329525/94‑1 (MQ=60)
            CACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGA                                                                                  <  SRR4302542.603744/94‑1 (MQ=60)
            CACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGA                                                                                  <  SRR4302542.5511260/94‑1 (MQ=60)
             ACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGAC                                                                                 <  SRR4302542.4403763/94‑1 (MQ=60)
             ACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGAC                                                                                 >  SRR4302542.4963129/1‑94 (MQ=60)
              CTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACT                                                                                <  SRR4302542.6864214/94‑1 (MQ=60)
              CTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACT                                                                                <  SRR4302542.1082236/94‑1 (MQ=60)
                GGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTT                                                                              <  SRR4302542.34330/94‑1 (MQ=60)
                   AATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACC                                                                           <  SRR4302542.4395342/94‑1 (MQ=60)
                   AATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACC                                                                           >  SRR4302542.782015/1‑94 (MQ=60)
                    ATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCG                                                                          <  SRR4302542.2301097/94‑1 (MQ=60)
                    ATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCG                                                                          >  SRR4302542.2592327/1‑94 (MQ=60)
                      CAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCC                                                                        >  SRR4302542.3126055/1‑94 (MQ=60)
                       AGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCT                                                                       <  SRR4302542.6545966/94‑1 (MQ=60)
                        GTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTT                                                                      <  SRR4302542.4172051/94‑1 (MQ=60)
                        GTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTT                                                                      <  SRR4302542.5315658/94‑1 (MQ=60)
                        GTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTT                                                                      <  SRR4302542.597597/94‑1 (MQ=60)
                        GTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTT                                                                      <  SRR4302542.687164/94‑1 (MQ=60)
                        GTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTT                                                                      >  SRR4302542.1671795/1‑94 (MQ=60)
                          GGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTAC                                                                    <  SRR4302542.5102017/94‑1 (MQ=60)
                          GGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTAC                                                                    >  SRR4302542.6950125/1‑94 (MQ=60)
                           GCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACG                                                                   <  SRR4302542.4258605/94‑1 (MQ=60)
                           GCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACG                                                                   <  SRR4302542.5426973/94‑1 (MQ=60)
                           GCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACG                                                                   <  SRR4302542.4829212/94‑1 (MQ=60)
                            CCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGC                                                                  >  SRR4302542.2723992/1‑94 (MQ=60)
                             CTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCT                                                                 >  SRR4302542.5287121/1‑94 (MQ=60)
                             CTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCT                                                                 <  SRR4302542.2064954/94‑1 (MQ=60)
                             CTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCT                                                                 >  SRR4302542.4675773/1‑94 (MQ=60)
                              TGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTG                                                                <  SRR4302542.2537453/94‑1 (MQ=60)
                              TGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTG                                                                <  SRR4302542.3080341/94‑1 (MQ=60)
                                GTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCA                                                              >  SRR4302542.1655715/1‑94 (MQ=60)
                                 TTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCAT                                                             <  SRR4302542.6327892/94‑1 (MQ=60)
                                   GTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTT                                                           >  SRR4302542.2183089/1‑94 (MQ=60)
                                   GTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTT                                                           <  SRR4302542.3665947/94‑1 (MQ=60)
                                   GTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTT                                                           >  SRR4302542.5748464/1‑94 (MQ=60)
                                    TGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTT                                                          <  SRR4302542.1823453/94‑1 (MQ=60)
                                     GCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTC                                                         >  SRR4302542.1611924/1‑94 (MQ=60)
                                     GCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTC                                                         <  SRR4302542.4196637/94‑1 (MQ=60)
                                      CTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCG                                                        >  SRR4302542.2430487/1‑94 (MQ=60)
                                      CTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCG                                                        >  SRR4302542.3287521/1‑94 (MQ=60)
                                       TCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGG                                                       <  SRR4302542.5073783/94‑1 (MQ=60)
                                       TCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGG                                                       <  SRR4302542.144967/94‑1 (MQ=60)
                                        CACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGC                                                      >  SRR4302542.3157436/1‑94 (MQ=60)
                                         ACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCG                                                     >  SRR4302542.4619188/1‑94 (MQ=60)
                                           ACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCT                                                   >  SRR4302542.5338818/1‑94 (MQ=60)
                                           ACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCT                                                   >  SRR4302542.4572295/1‑94 (MQ=60)
                                            CTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTC                                                  >  SRR4302542.1507325/1‑94 (MQ=60)
                                            CTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTC                                                  <  SRR4302542.2458049/94‑1 (MQ=60)
                                            CTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTC                                                  >  SRR4302542.2979205/1‑94 (MQ=60)
                                             TTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCT                                                 <  SRR4302542.1865213/94‑1 (MQ=60)
                                             TTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCT                                                 >  SRR4302542.6432463/1‑94 (MQ=60)
                                              TATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTC                                                >  SRR4302542.7210837/1‑94 (MQ=60)
                                              TATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTC                                                <  SRR4302542.5504820/94‑1 (MQ=60)
                                               ATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCG                                               >  SRR4302542.7071259/1‑94 (MQ=60)
                                                  TGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTT                                            >  SRR4302542.402317/1‑94 (MQ=60)
                                                  TGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTT                                            <  SRR4302542.3838670/94‑1 (MQ=60)
                                                   GGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTT                                           <  SRR4302542.3823712/94‑1 (MQ=60)
                                                   GGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTT                                           <  SRR4302542.6474030/94‑1 (MQ=60)
                                                    GAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTA                                          >  SRR4302542.2291471/1‑94 (MQ=60)
                                                      GTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATT                                        <  SRR4302542.761642/94‑1 (MQ=60)
                                                      GTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATT                                        >  SRR4302542.3122056/1‑94 (MQ=60)
                                                       TTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTC                                       <  SRR4302542.1184887/94‑1 (MQ=60)
                                                       TTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTC                                       <  SRR4302542.6258683/94‑1 (MQ=60)
                                                       TTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTC                                       >  SRR4302542.6902142/1‑94 (MQ=60)
                                                       TTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTC                                       <  SRR4302542.2689791/94‑1 (MQ=60)
                                                        TATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCA                                      >  SRR4302542.598056/1‑94 (MQ=60)
                                                         ATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCAT                                     <  SRR4302542.1924112/94‑1 (MQ=60)
                                                          TAGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATC                                    >  SRR4302542.3019588/1‑94 (MQ=60)
                                                           AGCTGGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCG                                   <  SRR4302542.6697722/94‑1 (MQ=60)
                                                           AGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCG                                   >  SRR4302542.4856265/1‑94 (MQ=60)
                                                               GGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCT                               <  SRR4302542.2811266/94‑1 (MQ=60)
                                                               GGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCT                               >  SRR4302542.3636462/1‑94 (MQ=60)
                                                               GGATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCT                               <  SRR4302542.6817505/94‑1 (MQ=60)
                                                                 ATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTT                             >  SRR4302542.6140797/1‑94 (MQ=60)
                                                                 ATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTT                             <  SRR4302542.5262391/94‑1 (MQ=60)
                                                                 ATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTT                             >  SRR4302542.2348750/1‑94 (MQ=60)
                                                                 ATTTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTT                             >  SRR4302542.5712925/1‑94 (MQ=60)
                                                                  TTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTT                            >  SRR4302542.4492922/1‑94 (MQ=60)
                                                                  TTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTT                            <  SRR4302542.889987/94‑1 (MQ=60)
                                                                   TTTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTA                           <  SRR4302542.3657577/94‑1 (MQ=60)
                                                                   TTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTA                           >  SRR4302542.5965681/1‑94 (MQ=60)
                                                                    tcaATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTAT                          <  SRR4302542.2439743/91‑1 (MQ=60)
                                                                    TTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTAT                          <  SRR4302542.880078/94‑1 (MQ=60)
                                                                    TTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTAT                          <  SRR4302542.5639198/94‑1 (MQ=60)
                                                                    TTCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTAT                          <  SRR4302542.7306474/94‑1 (MQ=60)
                                                                     TCATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCCTTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATT                         >  SRR4302542.2613454/1‑94 (MQ=60)
                                                                      CATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTA                        <  SRR4302542.710016/94‑1 (MQ=60)
                                                                      CATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTA                        >  SRR4302542.3218627/1‑94 (MQ=60)
                                                                      CATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTA                        <  SRR4302542.1555702/94‑1 (MQ=60)
                                                                       ATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTAC                       >  SRR4302542.2011103/1‑94 (MQ=60)
                                                                       ATGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTAC                       <  SRR4302542.2142079/94‑1 (MQ=60)
                                                                        TGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACG                      <  SRR4302542.4353019/94‑1 (MQ=60)
                                                                        TGAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACG                      <  SRR4302542.5780185/94‑1 (MQ=60)
                                                                         GAAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGT                     >  SRR4302542.2900493/1‑94 (MQ=60)
                                                                          AAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTG                    <  SRR4302542.5109526/94‑1 (MQ=60)
                                                                          AAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTG                    <  SRR4302542.3287120/94‑1 (MQ=60)
                                                                          AAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTG                    <  SRR4302542.6895351/94‑1 (MQ=60)
                                                                          AAGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTG                    <  SRR4302542.7597216/94‑1 (MQ=60)
                                                                          AAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTG                    <  SRR4302542.4283454/94‑1 (MQ=60)
                                                                           AGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGG                   <  SRR4302542.6285753/94‑1 (MQ=60)
                                                                           AGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGG                   <  SRR4302542.5465271/94‑1 (MQ=60)
                                                                           AGCAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGG                   <  SRR4302542.3935539/94‑1 (MQ=60)
                                                                             CAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTC                 <  SRR4302542.3012800/94‑1 (MQ=60)
                                                                             CAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTC                 <  SRR4302542.1692757/94‑1 (MQ=60)
                                                                             CAAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTC                 >  SRR4302542.4201472/1‑94 (MQ=60)
                                                                              AAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCG                <  SRR4302542.1385231/94‑1 (MQ=60)
                                                                              AAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCG                <  SRR4302542.6183610/94‑1 (MQ=60)
                                                                              AAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCG                <  SRR4302542.3457993/94‑1 (MQ=60)
                                                                              AAGTCACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCG                <  SRR4302542.6901684/94‑1 (MQ=60)
                                                                               AGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGC               >  SRR4302542.6672609/1‑94 (MQ=60)
                                                                                  CACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGA            >  SRR4302542.5802422/1‑94 (MQ=60)
                                                                                  CACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGA            >  SRR4302542.2293559/1‑94 (MQ=60)
                                                                                   ACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAA           >  SRR4302542.1064381/1‑94 (MQ=60)
                                                                                   ACCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAA           <  SRR4302542.1376570/94‑1 (MQ=60)
                                                                                    CCAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAAT          >  SRR4302542.5559570/1‑94 (MQ=60)
                                                                                    CCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATCACGTGGTCGCGGAAT          <  SRR4302542.7565924/94‑1 (MQ=60)
                                                                                     CAGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATG         <  SRR4302542.5385900/94‑1 (MQ=60)
                                                                                     CAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATG         >  SRR4302542.987484/1‑94 (MQ=60)
                                                                                      AGTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGC        <  SRR4302542.2856925/94‑1 (MQ=60)
                                                                                      AGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGC        >  SRR4302542.6691198/1‑94 (MQ=60)
                                                                                       GTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCG       <  SRR4302542.3803408/94‑1 (MQ=60)
                                                                                       GTTACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCG       <  SRR4302542.1334739/94‑1 (MQ=60)
                                                                                          ACGTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCC    >  SRR4302542.5087397/1‑94 (MQ=60)
                                                                                          ACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCC    >  SRR4302542.4112442/1‑94 (MQ=60)
                                                                                           CATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCG   >  SRR4302542.1269922/1‑94 (MQ=60)
                                                                                            GTCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGA  >  SRR4302542.2534253/1‑94 (MQ=60)
                                                                                            |                                                                                             
GAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGA  >  NC_000913/4133701‑4133886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑