Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,182,169 A→G H1116R (CAC→CGC)  rpoB → RNA polymerase beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,1690AG99.7% 947.5 / ‑4.8 286H1116R (CAC→CGC) rpoBRNA polymerase beta subunit
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (150/134);  total (152/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 286 total aligned reads displayed.

CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4182079‑4182258
                                                                                           |                                                                                         
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGcc                                                                                         >  1:2226703/1‑93 (MQ=255)
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGcc                                                                                         >  1:2614360/1‑93 (MQ=255)
 ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCt                                                                                        >  2:7341314/1‑93 (MQ=255)
 ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCt                                                                                        >  2:1268886/1‑93 (MQ=255)
 ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCt                                                                                        >  1:3915633/1‑93 (MQ=255)
  tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTg                                                                                       <  2:707031/93‑1 (MQ=255)
  tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTg                                                                                       <  2:3413248/93‑1 (MQ=255)
   aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTgg                                                                                      <  1:7500073/93‑1 (MQ=255)
    cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTggg                                                                                     >  1:938512/1‑93 (MQ=255)
     gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGt                                                                                    >  1:126991/1‑93 (MQ=255)
     gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGt                                                                                    >  2:4488848/1‑93 (MQ=255)
      aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTa                                                                                   <  2:2996563/93‑1 (MQ=255)
      aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTa                                                                                   >  1:3604865/1‑93 (MQ=255)
      aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTa                                                                                   <  2:2124580/93‑1 (MQ=255)
      aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGGCAGATCCTCGAAAC‑CGGCCTGGGtt                                                                                   >  1:2019055/1‑92 (MQ=255)
       tGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTAt                                                                                  <  1:6711968/93‑1 (MQ=255)
       tGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTAt                                                                                  >  1:357255/1‑93 (MQ=255)
        gAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATg                                                                                 >  1:2539760/1‑93 (MQ=255)
        gAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATg                                                                                 <  2:3617601/93‑1 (MQ=255)
           aaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCt                                                                              >  1:7010131/1‑93 (MQ=255)
           aaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCt                                                                              <  1:1606943/93‑1 (MQ=255)
              ggTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCg                                                                           >  1:4096954/1‑93 (MQ=255)
                 aCGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGaaa                                                                        <  1:5945019/93‑1 (MQ=255)
                  cGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAg                                                                       >  2:1880262/1‑93 (MQ=255)
                      ggTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTAt                                                                   <  2:4565481/93‑1 (MQ=255)
                      ggTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTAt                                                                   >  1:3462735/1‑93 (MQ=255)
                       gTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATc                                                                  >  2:2529918/1‑93 (MQ=255)
                        tAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGGAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCg                                                                 >  2:4062186/1‑93 (MQ=255)
                         aGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCgg                                                                >  2:5859537/1‑93 (MQ=255)
                          gACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGc                                                               <  1:1614709/93‑1 (MQ=255)
                           aCATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCg                                                              <  1:567158/93‑1 (MQ=255)
                            cATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                             >  2:949370/1‑93 (MQ=255)
                              tCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACa                                                           >  2:4383186/1‑93 (MQ=255)
                              tCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACa                                                           >  1:5210696/1‑93 (MQ=255)
                                gTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                         >  2:798260/1‑93 (MQ=255)
                                  aCTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                       <  2:6151440/93‑1 (MQ=255)
                                  aCTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                       <  1:6627503/93‑1 (MQ=255)
                                   cTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                      <  2:6504202/93‑1 (MQ=255)
                                   cTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                      <  1:2913680/93‑1 (MQ=255)
                                    tGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                     >  2:4969740/1‑93 (MQ=255)
                                     gAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                    >  2:6314238/1‑93 (MQ=255)
                                     gAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                    >  2:545549/1‑93 (MQ=255)
                                      aaCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                   >  1:2823522/1‑93 (MQ=255)
                                      aaCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                   <  2:4685649/93‑1 (MQ=255)
                                       aCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACg                                                  <  1:6682951/93‑1 (MQ=255)
                                       aCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACg                                                  >  2:2777898/1‑93 (MQ=255)
                                        cccGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                 >  2:6385910/1‑93 (MQ=255)
                                        cccGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                 <  2:4113447/93‑1 (MQ=255)
                                         ccGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                                >  1:1434850/1‑93 (MQ=255)
                                         ccGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                                                >  2:2401290/1‑93 (MQ=255)
                                          cGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                                               >  1:800818/1‑93 (MQ=255)
                                          cGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                                               <  2:4805399/93‑1 (MQ=255)
                                           gCTGGGCGTTCCTGCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAt                                              <  1:5071036/93‑1 (MQ=255)
                                             tGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGc                                            >  1:1469728/1‑93 (MQ=255)
                                                  gTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       >  2:1515458/1‑93 (MQ=255)
                                                  gTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       <  2:4151663/93‑1 (MQ=255)
                                                  gTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       >  1:7067851/1‑93 (MQ=255)
                                                  gTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       <  2:457995/93‑1 (MQ=255)
                                                  gTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCTGCGGCAAGATCAACGCCATGCTGaaa                                       >  1:7324003/1‑93 (MQ=255)
                                                   tACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                      <  2:37325/93‑1 (MQ=255)
                                                   tACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                      >  1:1852655/1‑93 (MQ=255)
                                                   tACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                      >  1:4789370/1‑93 (MQ=255)
                                                    aCCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAca                                     <  1:7144377/93‑1 (MQ=255)
                                                     ccGTCTCGTATGAACATCGGTCAGATCCTCGAAACGCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAca                                     <  1:3718685/93‑1 (MQ=255)
                                                     ccGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                    <  1:4673230/93‑1 (MQ=255)
                                                     ccGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                    >  2:3226383/1‑93 (MQ=255)
                                                     ccGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                    >  2:3958732/1‑93 (MQ=255)
                                                     ccGACTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGACCAACGCCATGCTGAAAcag                                    <  1:1162291/93‑1 (MQ=255)
                                                      cGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                   >  2:2964420/1‑93 (MQ=255)
                                                      cGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                   >  2:2907196/1‑93 (MQ=255)
                                                       gTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                  >  2:6032045/1‑93 (MQ=255)
                                                        tctcGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                 <  2:6576949/93‑1 (MQ=255)
                                                           cGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                              >  2:7507883/1‑93 (MQ=255)
                                                           cGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                              <  2:1605874/93‑1 (MQ=255)
                                                             tATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                            <  2:1431815/93‑1 (MQ=255)
                                                             tATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                            >  1:6534059/1‑93 (MQ=255)
                                                             tATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                            >  1:1717373/1‑93 (MQ=255)
                                                             tATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                            >  2:5592410/1‑93 (MQ=255)
                                                              aTGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  2:7144828/93‑1 (MQ=255)
                                                                   cATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                      >  2:1719656/1‑93 (MQ=255)
                                                                   cATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                      <  2:5190346/93‑1 (MQ=255)
                                                                    aTCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgcg                     >  2:3773760/1‑93 (MQ=255)
                                                                    aTCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgcg                     <  1:3284441/93‑1 (MQ=255)
                                                                        gTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                 <  1:6021514/93‑1 (MQ=255)
                                                                        gTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                 <  2:1116710/93‑1 (MQ=255)
                                                                        gTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                 >  2:5219236/1‑93 (MQ=255)
                                                                        gTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                 >  2:2262412/1‑93 (MQ=255)
                                                                        gTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATAGGCGACAAGAGCAACGCCATGCTGAAAAACCAGCAAGAAATCGCGAAAc                 >  1:6184481/1‑93 (MQ=255)
                                                                             aTCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc            >  1:42496/1‑93 (MQ=255)
                                                                             aTCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc            >  1:2792437/1‑93 (MQ=255)
                                                                              tCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg           >  1:1816219/1‑93 (MQ=255)
                                                                              tCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg           >  2:6752727/1‑93 (MQ=255)
                                                                                 tCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt        >  1:2168553/1‑93 (MQ=255)
                                                                                 tCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt        <  1:5339123/93‑1 (MQ=255)
                                                                                 tCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt        <  1:4409475/93‑1 (MQ=255)
                                                                                  cGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt       <  1:3016186/93‑1 (MQ=255)
                                                                                    aaaC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa     <  1:4597355/93‑1 (MQ=255)
                                                                                      aC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc   >  2:5938874/1‑93 (MQ=255)
                                                                                      aC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc   <  2:2705593/93‑1 (MQ=255)
                                                                                      aC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc   >  2:3019476/1‑93 (MQ=255)
                                                                                           |                                                                                         
CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4182079‑4182258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  >  NC_000913/4182076‑4182261
                                                                                              |                                                                                            
TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCG                                                                                              <  SRR4302542.5251885/94‑1 (MQ=60)
 GCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGC                                                                                             >  SRR4302542.377315/1‑94 (MQ=60)
  CCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCC                                                                                            >  SRR4302542.2245864/1‑94 (MQ=60)
  CCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCC                                                                                            <  SRR4302542.1386113/94‑1 (MQ=60)
  CCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCC                                                                                            >  SRR4302542.3795128/1‑94 (MQ=60)
  CCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCC                                                                                            >  SRR4302542.2639619/1‑94 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCT                                                                                           <  SRR4302542.6055223/94‑1 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCT                                                                                           >  SRR4302542.5839519/1‑94 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCT                                                                                           >  SRR4302542.3160367/1‑94 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCT                                                                                           >  SRR4302542.3092778/1‑94 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCT                                                                                           >  SRR4302542.3954271/1‑94 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCT                                                                                           >  SRR4302542.5206937/1‑94 (MQ=60)
      ACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGG                                                                                        <  SRR4302542.7573839/94‑1 (MQ=60)
      ACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGG                                                                                        >  SRR4302542.946595/1‑94 (MQ=60)
       CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGT                                                                                       >  SRR4302542.128028/1‑94 (MQ=60)
       CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGG                                                                                       >  SRR4302542.3741150/1‑94 (MQ=60)
        GATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTA                                                                                      >  SRR4302542.4887366/1‑94 (MQ=60)
        GATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTA                                                                                      >  SRR4302542.3638456/1‑94 (MQ=60)
        GATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTA                                                                                      <  SRR4302542.1838212/94‑1 (MQ=60)
         ATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTAT                                                                                     >  SRR4302542.1479974/1‑94 (MQ=60)
         ATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTAT                                                                                     <  SRR4302542.7024497/94‑1 (MQ=60)
         ATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTAT                                                                                     >  SRR4302542.360371/1‑94 (MQ=60)
          TGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATG                                                                                    <  SRR4302542.6778083/94‑1 (MQ=60)
          TGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATG                                                                                    >  SRR4302542.2564384/1‑94 (MQ=60)
           GAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGG                                                                                   >  SRR4302542.3942901/1‑94 (MQ=60)
           GAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGCATGG                                                                                   >  SRR4302542.1370446/1‑94 (MQ=60)
             AAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCT                                                                                 >  SRR4302542.7078987/1‑94 (MQ=60)
              AACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTG                                                                                <  SRR4302542.1620821/94‑1 (MQ=60)
                CGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCG                                                                              <  SRR4302542.2185905/94‑1 (MQ=60)
                CGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCG                                                                              <  SRR4302542.7583734/94‑1 (MQ=60)
                CGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCG                                                                              >  SRR4302542.4137366/1‑94 (MQ=60)
                 GGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGG                                                                             >  SRR4302542.2197600/1‑94 (MQ=60)
                 GGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGG                                                                             >  SRR4302542.5450601/1‑94 (MQ=60)
                    ACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAG                                                                          <  SRR4302542.20420/94‑1 (MQ=60)
                    ACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAG                                                                          <  SRR4302542.6002604/94‑1 (MQ=60)
                       CCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTA                                                                       <  SRR4302542.6661811/94‑1 (MQ=60)
                       CCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGA                                                                       >  SRR4302542.6476237/1‑94 (MQ=60)
                        CGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTAT                                                                      >  SRR4302542.3495164/1‑94 (MQ=60)
                        CGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTAT                                                                      <  SRR4302542.2584023/94‑1 (MQ=60)
                         NNTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATC                                                                     <  SRR4302542.1626990/94‑1 (MQ=60)
                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATC                                                                     >  SRR4302542.332503/1‑94 (MQ=60)
                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATC                                                                     <  SRR4302542.6593053/94‑1 (MQ=60)
                           TAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGG                                                                   <  SRR4302542.1410201/94‑1 (MQ=60)
                            AGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGC                                                                  <  SRR4302542.5321004/94‑1 (MQ=60)
                             GACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCG                                                                 <  SRR4302542.1628658/94‑1 (MQ=60)
                              ACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGA                                                                <  SRR4302542.4829072/94‑1 (MQ=60)
                              ACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGA                                                                <  SRR4302542.572037/94‑1 (MQ=60)
                               CATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGAC                                                               >  SRR4302542.3412024/1‑94 (MQ=60)
                                ATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACA                                                              <  SRR4302542.1793658/94‑1 (MQ=60)
                                ATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACA                                                              >  SRR4302542.4321679/1‑94 (MQ=60)
                                ATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACA                                                              >  SRR4302542.5520181/1‑94 (MQ=60)
                                ATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACA                                                              >  SRR4302542.5261370/1‑94 (MQ=60)
                                    TACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGAT                                                          >  SRR4302542.2909029/1‑94 (MQ=60)
                                     ACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATC                                                         >  SRR4302542.908753/1‑94 (MQ=60)
                                     ACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATC                                                         >  SRR4302542.2436203/1‑94 (MQ=60)
                                     ACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATC                                                         <  SRR4302542.6692845/94‑1 (MQ=60)
                                     ACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATC                                                         <  SRR4302542.5961765/94‑1 (MQ=60)
                                      CTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCA                                                        <  SRR4302542.2941626/94‑1 (MQ=60)
                                      CTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCA                                                        <  SRR4302542.3520302/94‑1 (MQ=60)
                                       TGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAA                                                       <  SRR4302542.127803/94‑1 (MQ=60)
                                        GAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAC                                                      >  SRR4302542.2850670/1‑94 (MQ=60)
                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACG                                                     <  SRR4302542.498704/94‑1 (MQ=60)
                                          ACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGC                                                    <  SRR4302542.6748776/94‑1 (MQ=60)
                                           CCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCC                                                   >  SRR4302542.1447244/1‑94 (MQ=60)
                                            CCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCA                                                  >  SRR4302542.807702/1‑94 (MQ=60)
                                            CCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCA                                                  >  SRR4302542.7298384/1‑94 (MQ=60)
                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAT                                                 >  SRR4302542.1911425/1‑94 (MQ=60)
                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAT                                                 <  SRR4302542.846915/94‑1 (MQ=60)
                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAT                                                 <  SRR4302542.7205667/94‑1 (MQ=60)
                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAT                                                 <  SRR4302542.6739180/94‑1 (MQ=60)
                                              GCTGGGCGTTCCTGCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATG                                                <  SRR4302542.5120312/94‑1 (MQ=60)
                                              GCTGGGCGTACCGGCTCGTATGACCATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATG                                                <  SRR4302542.2428680/94‑1 (MQ=60)
                                               CTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGC                                               <  SRR4302542.1720140/94‑1 (MQ=60)
                                               CTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGC                                               >  SRR4302542.1482417/1‑94 (MQ=60)
                                                TGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCT                                              <  SRR4302542.3636808/94‑1 (MQ=60)
                                                TGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCT                                              >  SRR4302542.2849369/1‑94 (MQ=60)
                                                TGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCT                                              <  SRR4302542.3007114/94‑1 (MQ=60)
                                                   GCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAA                                           <  SRR4302542.7087907/94‑1 (MQ=60)
                                                    CGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAA                                          >  SRR4302542.1508796/1‑94 (MQ=60)
                                                    CGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAA                                          >  SRR4302542.7137223/1‑94 (MQ=60)
                                                    CGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAA                                          >  SRR4302542.4534292/1‑94 (MQ=60)
                                                    CGTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCTGCGGCAAGATCAACGCCATGCTGAAA                                          >  SRR4302542.7395738/1‑94 (MQ=60)
                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                         >  SRR4302542.1626106/1‑94 (MQ=60)
                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                         >  SRR4302542.1868675/1‑94 (MQ=60)
                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                         >  SRR4302542.4836041/1‑94 (MQ=60)
                                                      TACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACA                                        <  SRR4302542.30266/94‑1 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       >  SRR4302542.6772339/1‑94 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302542.5257344/94‑1 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302542.6028972/94‑1 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302542.7214486/94‑1 (MQ=60)
                                                        CCGTCTCGTATGAACATCGGTCAGATCCTCGAAACGCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302542.3755475/94‑1 (MQ=60)
                                                        CCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGC                                      <  SRR4302542.4718895/94‑1 (MQ=60)
                                                        CCGACTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGACCAACGCCATGCTGAAACAGC                                      <  SRR4302542.1172276/94‑1 (MQ=60)
                                                          GTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAG                                    >  SRR4302542.1723689/1‑94 (MQ=60)
                                                            CTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCA                                  <  SRR4302542.4427745/94‑1 (MQ=60)
                                                              CGTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAG                                <  SRR4302542.6743462/94‑1 (MQ=60)
                                                               GTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGA                               >  SRR4302542.6598463/1‑94 (MQ=60)
                                                               GTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGA                               <  SRR4302542.2650327/94‑1 (MQ=60)
                                                               GTATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGA                               >  SRR4302542.1732286/1‑94 (MQ=60)
                                                                TATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAA                              >  SRR4302542.270708/1‑94 (MQ=60)
                                                                TATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCACCAAGAA                              <  SRR4302542.3574837/94‑1 (MQ=60)
                                                                 ATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                             >  SRR4302542.2238339/1‑94 (MQ=60)
                                                                 ATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                             <  SRR4302542.46993/94‑1 (MQ=60)
                                                                 ATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                             <  SRR4302542.3987249/94‑1 (MQ=60)
                                                                 ATGAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                             <  SRR4302542.3956681/94‑1 (MQ=60)
                                                                   GAACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTC                           >  SRR4302542.1464584/1‑94 (MQ=60)
                                                                    AACATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                          <  SRR4302542.4150200/94‑1 (MQ=60)
                                                                      CATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCG                        <  SRR4302542.1490819/94‑1 (MQ=60)
                                                                      CATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCG                        >  SRR4302542.7112651/1‑94 (MQ=60)
                                                                       ATCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGA                       <  SRR4302542.3315418/94‑1 (MQ=60)
                                                                        TCGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAA                      >  SRR4302542.2582843/1‑94 (MQ=60)
                                                                         CGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAA                     >  SRR4302542.1755653/1‑94 (MQ=60)
                                                                         CGGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAA                     >  SRR4302542.286876/1‑94 (MQ=60)
                                                                          GGTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGGATAGGCGACAAGAGCAACGCCATGCTGAAAAACCAGCAAGAAATCGCGAAAC                    >  SRR4302542.6244501/1‑94 (MQ=60)
                                                                           GTCAGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACT                   <  SRR4302542.6079829/94‑1 (MQ=60)
                                                                              AGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCG                >  SRR4302542.767936/1‑94 (MQ=60)
                                                                              AGATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCG                >  SRR4302542.7513818/1‑94 (MQ=60)
                                                                               GATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGC               >  SRR4302542.2819311/1‑94 (MQ=60)
                                                                               GATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGC               >  SRR4302542.42866/1‑94 (MQ=60)
                                                                                ATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG              >  SRR4302542.1831952/1‑94 (MQ=60)
                                                                                ATCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG              >  SRR4302542.4228537/1‑94 (MQ=60)
                                                                                 TCCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGA             >  SRR4302542.7238547/1‑94 (MQ=60)
                                                                                  CCTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAA            >  SRR4302542.4508248/1‑94 (MQ=60)
                                                                                   CTCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAT           >  SRR4302542.2187227/1‑94 (MQ=60)
                                                                                    TCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATT          <  SRR4302542.7566323/94‑1 (MQ=60)
                                                                                    TCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATT          <  SRR4302542.5390998/94‑1 (MQ=60)
                                                                                    TCGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATT          <  SRR4302542.4452778/94‑1 (MQ=60)
                                                                                     CGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC         >  SRR4302542.6805916/1‑94 (MQ=60)
                                                                                     CGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC         >  SRR4302542.4704143/1‑94 (MQ=60)
                                                                                     CGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC         <  SRR4302542.3045009/94‑1 (MQ=60)
                                                                                     CGAAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC         <  SRR4302542.5273296/94‑1 (MQ=60)
                                                                                       AAAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT       <  SRR4302542.4642303/94‑1 (MQ=60)
                                                                                        AAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC      >  SRR4302542.6614187/1‑94 (MQ=60)
                                                                                        AAC‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC      >  SRR4302542.3608814/1‑94 (MQ=60)
                                                                                        AAC‑CCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC      >  SRR4302542.6763097/1‑94 (MQ=60)
                                                                                          C‑CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA    <  SRR4302542.1955287/94‑1 (MQ=60)
                                                                                            CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG   >  SRR4302542.287067/1‑94 (MQ=60)
                                                                                             CGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  <  SRR4302542.7596149/94‑1 (MQ=60)
                                                                                              |                                                                                            
TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAC‑CCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  >  NC_000913/4182076‑4182261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑