Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1061 294.1 15065038 98.6% 14854127 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,527,603 A→G L70P (CTA→CCA)  sgcC ← putative PTS system enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,527,6030AG83.2% 692.4 / 143.6 322L70P (CTA→CCA) sgcCputative PTS system enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base G (149/119);  total (176/146)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 324 total aligned reads displayed.

AAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCA  >  NC_000913/4527513‑4527695
                                                                                          |                                                                                            
aaaaTAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTggg                                                                                            >  1:2810817/1‑93 (MQ=255)
 aaaTAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGc                                                                                           >  2:6681338/1‑93 (MQ=255)
    tAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGa                                                                                        <  1:1764539/93‑1 (MQ=255)
       cTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAAc                                                                                     >  1:734920/1‑93 (MQ=255)
       cTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAAc                                                                                     >  2:6151523/1‑93 (MQ=255)
       cTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAAc                                                                                     <  1:1024600/93‑1 (MQ=255)
       cTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAAc                                                                                     <  2:5365702/93‑1 (MQ=255)
        tGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACg                                                                                    >  1:3112931/1‑93 (MQ=255)
          ggATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCt                                                                                  >  2:4896749/1‑93 (MQ=255)
             tAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCaa                                                                               >  2:4604091/1‑93 (MQ=255)
              aaTCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAat                                                                              <  2:2345918/93‑1 (MQ=255)
              aaTCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAat                                                                              <  2:1237595/93‑1 (MQ=255)
               aTCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAatc                                                                             >  1:3339938/1‑93 (MQ=255)
               aTCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAatc                                                                             >  2:1559924/1‑93 (MQ=255)
                tCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAatca                                                                            <  2:2889549/93‑1 (MQ=255)
                tCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAatca                                                                            >  1:5467950/1‑93 (MQ=255)
                tCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAatca                                                                            <  1:3213585/93‑1 (MQ=255)
                  aTGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAatcatc                                                                          >  2:2026639/1‑93 (MQ=255)
                    ggCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCAc                                                                        <  2:5669778/93‑1 (MQ=255)
                     gcgcCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACt                                                                       >  1:5864129/1‑93 (MQ=255)
                      cgcCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACtt                                                                      <  1:6885410/93‑1 (MQ=255)
                       gcCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACttt                                                                     >  2:6910687/1‑93 (MQ=255)
                        ccAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTg                                                                    >  2:748064/1‑93 (MQ=255)
                        ccAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTg                                                                    >  1:6569949/1‑93 (MQ=255)
                         cAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGa                                                                   >  1:3805958/1‑93 (MQ=255)
                         cAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGa                                                                   <  1:1290325/93‑1 (MQ=255)
                         cAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGa                                                                   >  2:4125135/1‑93 (MQ=255)
                         cAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGa                                                                   <  1:4553787/93‑1 (MQ=255)
                          aaTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGAt                                                                  >  1:2924874/1‑93 (MQ=255)
                           aTGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATa                                                                 <  1:3281962/93‑1 (MQ=255)
                            tGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAg                                                                >  2:3174816/1‑93 (MQ=255)
                            tGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAg                                                                >  2:2679855/1‑93 (MQ=255)
                            tGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAg                                                                >  2:4801918/1‑93 (MQ=255)
                             ggCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAgg                                                               <  1:1584525/93‑1 (MQ=255)
                             ggCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAgg                                                               <  1:2083153/93‑1 (MQ=255)
                              gCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAggg                                                              <  1:4042641/93‑1 (MQ=255)
                               cTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAgggg                                                             <  1:5914972/93‑1 (MQ=255)
                               cTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAgggg                                                             >  2:7117273/1‑93 (MQ=255)
                                 gTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGa                                                           <  1:6251992/93‑1 (MQ=255)
                                     cATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGa                                                       >  1:4794890/1‑93 (MQ=255)
                                     cATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGa                                                       <  2:5759826/93‑1 (MQ=255)
                                     cATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGa                                                       <  1:926330/93‑1 (MQ=255)
                                       tAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAgg                                                     >  2:3842062/1‑93 (MQ=255)
                                       tAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAgg                                                     >  1:4310052/1‑93 (MQ=255)
                                        aaCCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGc                                                    >  2:4182600/1‑93 (MQ=255)
                                         aCCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCt                                                   >  2:5460075/1‑93 (MQ=255)
                                          ccAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTg                                                  >  1:2573105/1‑93 (MQ=255)
                                          ccAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTg                                                  <  1:7267081/93‑1 (MQ=255)
                                           cAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGt                                                 >  1:4230467/1‑93 (MQ=255)
                                            aaCGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTc                                                <  2:3058145/93‑1 (MQ=255)
                                            aaCGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTc                                                <  1:7125035/93‑1 (MQ=255)
                                            aaCGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTc                                                >  2:5260753/1‑93 (MQ=255)
                                            aaCGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTc                                                <  1:2220613/93‑1 (MQ=255)
                                            aaCGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTc                                                >  2:894171/1‑93 (MQ=255)
                                             aCGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCg                                               >  1:3550573/1‑93 (MQ=255)
                                               gCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGAt                                             <  1:6776830/93‑1 (MQ=255)
                                                      gCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATg                                      >  1:2438724/1‑93 (MQ=255)
                                                       cAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGa                                     <  1:6671057/93‑1 (MQ=255)
                                                        aGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGAc                                    >  2:2411634/1‑93 (MQ=255)
                                                         gggCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGGCTGAGGCTGTCGATCGCCATGACg                                   >  2:139423/1‑93 (MQ=255)
                                                          ggCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGa                                  <  2:5176431/93‑1 (MQ=255)
                                                          ggCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGa                                  <  2:4470463/93‑1 (MQ=255)
                                                          ggCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGa                                  >  2:7031974/1‑93 (MQ=255)
                                                           gCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGAt                                 <  1:5317710/93‑1 (MQ=255)
                                                           gCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGAt                                 <  2:2208141/93‑1 (MQ=255)
                                                             cTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCa                               <  1:4948686/93‑1 (MQ=255)
                                                             cTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCa                               >  2:6748882/1‑93 (MQ=255)
                                                              tGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCAc                              >  2:7365740/1‑93 (MQ=255)
                                                               gcgcCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCAcc                             <  2:5073283/93‑1 (MQ=255)
                                                                cgcCACCGTAAAAGACAGGCGGGGCGGGGCCAAACCGCTAAATCATCCTTTTGAGGGGGGGACGGGGGCTGTCGACCGCCATGACGATCACCa                            <  1:5110568/93‑1 (MQ=255)
                                                                cgcCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCa                            <  2:600627/93‑1 (MQ=255)
                                                                cgcCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCa                            <  1:2296434/93‑1 (MQ=255)
                                                                 gcCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAg                           <  1:6391910/93‑1 (MQ=255)
                                                                  ccAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGt                          >  1:1938007/1‑93 (MQ=255)
                                                                  ccAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGt                          >  2:2562784/1‑93 (MQ=255)
                                                                  ccAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGt                          >  1:3995572/1‑93 (MQ=255)
                                                                  ccAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGt                          <  1:1232251/93‑1 (MQ=255)
                                                                   cAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTc                         >  2:6276736/1‑93 (MQ=255)
                                                                   cAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTc                         >  2:1753937/1‑93 (MQ=255)
                                                                   cAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTc                         <  1:2642920/93‑1 (MQ=255)
                                                                   cAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTc                         >  1:4848257/1‑93 (MQ=255)
                                                                   cAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTc                         <  2:4071670/93‑1 (MQ=255)
                                                                    aaCGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTcc                        >  2:3370135/1‑93 (MQ=255)
                                                                        tCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATa                    <  1:4096603/93‑1 (MQ=255)
                                                                        tCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATa                    <  1:6075873/93‑1 (MQ=255)
                                                                        tCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATa                    <  2:7079651/93‑1 (MQ=255)
                                                                           aaGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCg                 >  2:3003228/1‑93 (MQ=255)
                                                                           aaGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCg                 <  1:1535917/93‑1 (MQ=255)
                                                                           aaGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCg                 >  1:6472081/1‑93 (MQ=255)
                                                                             gACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGAc               >  2:1896172/1‑93 (MQ=255)
                                                                             gACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGAc               <  1:3633102/93‑1 (MQ=255)
                                                                               cATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACaa             >  2:1651866/1‑93 (MQ=255)
                                                                                 tGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACaaaa           <  2:2786900/93‑1 (MQ=255)
                                                                                  gCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAAc          <  1:5048958/93‑1 (MQ=255)
                                                                                  gCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAAc          <  2:7167417/93‑1 (MQ=255)
                                                                                   cAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAAcc         <  2:944273/93‑1 (MQ=255)
                                                                                     gggCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCaa       <  1:352307/93‑1 (MQ=255)
                                                                                     gggCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCaa       >  1:113473/1‑93 (MQ=255)
                                                                                       gCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATc     <  2:2073188/93‑1 (MQ=255)
                                                                                        cTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATcc    >  1:1366656/1‑93 (MQ=255)
                                                                                          |                                                                                            
AAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCA  >  NC_000913/4527513‑4527695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAA  >  NC_000913/4527511‑4527696
                                                                                            |                                                                                             
GAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGG                                                                                              <  SRR4302542.1570488/94‑1 (MQ=60)
GAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGG                                                                                              <  SRR4302542.3195517/94‑1 (MQ=60)
 AAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGG                                                                                             >  SRR4302542.2837873/1‑94 (MQ=60)
   AAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCC                                                                                           >  SRR4302542.704351/1‑94 (MQ=60)
   AAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCC                                                                                           >  SRR4302542.4572344/1‑94 (MQ=60)
   AAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCC                                                                                           >  SRR4302542.5060190/1‑94 (MQ=60)
   AAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCC                                                                                           <  SRR4302542.4952279/94‑1 (MQ=60)
    AATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCG                                                                                          >  SRR4302542.2516501/1‑94 (MQ=60)
     ATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGA                                                                                         <  SRR4302542.4725734/94‑1 (MQ=60)
      TAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAA                                                                                        >  SRR4302542.3130005/1‑94 (MQ=60)
      TAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAA                                                                                        >  SRR4302542.2119452/1‑94 (MQ=60)
      TAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAA                                                                                        <  SRR4302542.1779845/94‑1 (MQ=60)
       AACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAA                                                                                       >  SRR4302542.2344249/1‑94 (MQ=60)
        ACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAAC                                                                                      <  SRR4302542.142983/94‑1 (MQ=60)
        ACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAAC                                                                                      <  SRR4302542.4486367/94‑1 (MQ=60)
        ACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAAC                                                                                      <  SRR4302542.6363190/94‑1 (MQ=60)
        ACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAAC                                                                                      >  SRR4302542.741291/1‑94 (MQ=60)
         CTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACG                                                                                     >  SRR4302542.3142508/1‑94 (MQ=60)
         CTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACG                                                                                     >  SRR4302542.1131215/1‑94 (MQ=60)
         CTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACG                                                                                     <  SRR4302542.1033427/94‑1 (MQ=60)
              ATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAA                                                                                >  SRR4302542.2202253/1‑94 (MQ=60)
              ATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAA                                                                                >  SRR4302542.3572100/1‑94 (MQ=60)
               TAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAAT                                                                               <  SRR4302542.1584496/94‑1 (MQ=60)
                AATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATC                                                                              <  SRR4302542.5331485/94‑1 (MQ=60)
                AATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATC                                                                              >  SRR4302542.3587071/1‑94 (MQ=60)
                AATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATC                                                                              >  SRR4302542.3371373/1‑94 (MQ=60)
                AATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATC                                                                              <  SRR4302542.7243990/94‑1 (MQ=60)
                 ATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCA                                                                             >  SRR4302542.3263386/1‑94 (MQ=60)
                 ATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCA                                                                             >  SRR4302542.5521065/1‑94 (MQ=60)
                 ATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCA                                                                             >  SRR4302542.6034577/1‑94 (MQ=60)
                  TCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCAT                                                                            <  SRR4302542.1039862/94‑1 (MQ=60)
                  TCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCAT                                                                            <  SRR4302542.1236978/94‑1 (MQ=60)
                  TCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCAT                                                                            <  SRR4302542.3244000/94‑1 (MQ=60)
                  TCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCAT                                                                            <  SRR4302542.5499276/94‑1 (MQ=60)
                    ATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCA                                                                          <  SRR4302542.3770349/94‑1 (MQ=60)
                    ATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCA                                                                          >  SRR4302542.1355226/1‑94 (MQ=60)
                    ATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCA                                                                          <  SRR4302542.3367035/94‑1 (MQ=60)
                     GGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCAC                                                                         <  SRR4302542.424445/94‑1 (MQ=60)
                      GGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACT                                                                        >  SRR4302542.5920984/1‑94 (MQ=60)
                      GGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACT                                                                        >  SRR4302542.4854504/1‑94 (MQ=60)
                        CGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTT                                                                      <  SRR4302542.3286017/94‑1 (MQ=60)
                        CGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTT                                                                      <  SRR4302542.6953037/94‑1 (MQ=60)
                         GCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTG                                                                     >  SRR4302542.6634691/1‑94 (MQ=60)
                         GCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTG                                                                     <  SRR4302542.5578523/94‑1 (MQ=60)
                          CCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGA                                                                    >  SRR4302542.5109238/1‑94 (MQ=60)
                          CCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGA                                                                    >  SRR4302542.3843622/1‑94 (MQ=60)
                          CCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGA                                                                    >  SRR4302542.735512/1‑94 (MQ=60)
                           CAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGAT                                                                   <  SRR4302542.1301441/94‑1 (MQ=60)
                           CAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGAT                                                                   >  SRR4302542.6582822/1‑94 (MQ=60)
                           CAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGAT                                                                   >  SRR4302542.230764/1‑94 (MQ=60)
                           CAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGAT                                                                   >  SRR4302542.2952897/1‑94 (MQ=60)
                           CAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGAT                                                                   <  SRR4302542.4598348/94‑1 (MQ=60)
                            AATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATA                                                                  >  SRR4302542.3559411/1‑94 (MQ=60)
                            AATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATA                                                                  >  SRR4302542.6684391/1‑94 (MQ=60)
                            AATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATA                                                                  >  SRR4302542.263322/1‑94 (MQ=60)
                            AATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATA                                                                  >  SRR4302542.467394/1‑94 (MQ=60)
                             ATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAG                                                                 <  SRR4302542.3593509/94‑1 (MQ=60)
                             ATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAG                                                                 <  SRR4302542.6270375/94‑1 (MQ=60)
                             ATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAG                                                                 <  SRR4302542.3312923/94‑1 (MQ=60)
                              TGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGG                                                                <  SRR4302542.1471894/94‑1 (MQ=60)
                              TGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGG                                                                <  SRR4302542.2266256/94‑1 (MQ=60)
                               GGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGG                                                               <  SRR4302542.2101144/94‑1 (MQ=60)
                               GGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGG                                                               <  SRR4302542.1598202/94‑1 (MQ=60)
                                GCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGG                                                              <  SRR4302542.2481244/94‑1 (MQ=60)
                                GCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGG                                                              <  SRR4302542.1991533/94‑1 (MQ=60)
                                GCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGG                                                              <  SRR4302542.4082530/94‑1 (MQ=60)
                                GCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGG                                                              <  SRR4302542.4498827/94‑1 (MQ=60)
                                 CTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGG                                                             <  SRR4302542.2326930/94‑1 (MQ=60)
                                 CTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGG                                                             <  SRR4302542.5972291/94‑1 (MQ=60)
                                  TGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGA                                                            >  SRR4302542.976574/1‑94 (MQ=60)
                                  TGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGA                                                            >  SRR4302542.6821086/1‑94 (MQ=60)
                                  TGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGA                                                            >  SRR4302542.6925029/1‑94 (MQ=60)
                                  TGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGA                                                            >  SRR4302542.6821087/1‑94 (MQ=60)
                                   GTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGAC                                                           <  SRR4302542.6313596/94‑1 (MQ=60)
                                    TCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACT                                                          <  SRR4302542.3390999/94‑1 (MQ=60)
                                     CGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTG                                                         >  SRR4302542.2951279/1‑94 (MQ=60)
                                      GCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGA                                                        >  SRR4302542.4841616/1‑94 (MQ=60)
                                      GCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGA                                                        >  SRR4302542.5534658/1‑94 (MQ=60)
                                       CATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAG                                                       <  SRR4302542.934302/94‑1 (MQ=60)
                                       CATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAG                                                       <  SRR4302542.2588366/94‑1 (MQ=60)
                                       CATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAG                                                       >  SRR4302542.7420990/1‑94 (MQ=60)
                                        ATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGG                                                      >  SRR4302542.4352414/1‑94 (MQ=60)
                                        ATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGG                                                      >  SRR4302542.6716065/1‑94 (MQ=60)
                                        ATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGG                                                      >  SRR4302542.1877392/1‑94 (MQ=60)
                                          AACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCT                                                    >  SRR4302542.6266147/1‑94 (MQ=60)
                                          AACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCT                                                    >  SRR4302542.318450/1‑94 (MQ=60)
                                          AACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCT                                                    <  SRR4302542.5370933/94‑1 (MQ=60)
                                           ACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTG                                                   >  SRR4302542.4046935/1‑94 (MQ=60)
                                           ACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTG                                                   >  SRR4302542.2598030/1‑94 (MQ=60)
                                           ACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTG                                                   >  SRR4302542.2640140/1‑94 (MQ=60)
                                            CCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGT                                                  <  SRR4302542.7338318/94‑1 (MQ=60)
                                            CCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGT                                                  <  SRR4302542.3488523/94‑1 (MQ=60)
                                            CCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGT                                                  >  SRR4302542.4272136/1‑94 (MQ=60)
                                            CCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGT                                                  >  SRR4302542.2041028/1‑94 (MQ=60)
                                             CAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTC                                                 <  SRR4302542.3121535/94‑1 (MQ=60)
                                             CAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTC                                                 >  SRR4302542.595574/1‑94 (MQ=60)
                                              aactgcgcAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCG                                                <  SRR4302542.6078430/86‑1 (MQ=60)
                                              AACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCG                                                <  SRR4302542.2239714/94‑1 (MQ=60)
                                              AACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCG                                                >  SRR4302542.3583764/1‑94 (MQ=60)
                                              AACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCG                                                <  SRR4302542.4882895/94‑1 (MQ=60)
                                              AACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCG                                                <  SRR4302542.7194963/94‑1 (MQ=60)
                                                CGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGAT                                              <  SRR4302542.4127784/94‑1 (MQ=60)
                                                 GCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATC                                             <  SRR4302542.2893190/94‑1 (MQ=60)
                                                 GCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATC                                             <  SRR4302542.1248106/94‑1 (MQ=60)
                                                 GCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATC                                             <  SRR4302542.6843497/94‑1 (MQ=60)
                                                  CCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCG                                            >  SRR4302542.4154995/1‑94 (MQ=60)
                                                   CGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGC                                           >  SRR4302542.4351244/1‑94 (MQ=60)
                                                    GGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCC                                          >  SRR4302542.2759551/1‑94 (MQ=60)
                                                    GGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCC                                          >  SRR4302542.728590/1‑94 (MQ=60)
                                                      AGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCAT                                        <  SRR4302542.5977637/94‑1 (MQ=60)
                                                      AGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCAT                                        <  SRR4302542.6567505/94‑1 (MQ=60)
                                                       GGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATG                                       >  SRR4302542.2462455/1‑94 (MQ=60)
                                                        GCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGA                                      >  SRR4302542.4035551/1‑94 (MQ=60)
                                                         CAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGAC                                     <  SRR4302542.6736779/94‑1 (MQ=60)
                                                         CAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGAC                                     <  SRR4302542.5004983/94‑1 (MQ=60)
                                                          AGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACG                                    >  SRR4302542.1844998/1‑94 (MQ=60)
                                                          AGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACG                                    >  SRR4302542.6520567/1‑94 (MQ=60)
                                                          AGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACG                                    >  SRR4302542.17486/1‑94 (MQ=60)
                                                             GCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATC                                 <  SRR4302542.5369366/94‑1 (MQ=60)
                                                             GCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATC                                 >  SRR4302542.1637034/1‑94 (MQ=60)
                                                             GCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATC                                 <  SRR4302542.533369/94‑1 (MQ=60)
                                                             GCCTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATC                                 >  SRR4302542.5147353/1‑94 (MQ=60)
                                                               CTGCGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCAC                               <  SRR4302542.4996766/94‑1 (MQ=60)
                                                                  CGCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAG                            <  SRR4302542.2316165/94‑1 (MQ=60)
                                                                  CGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAG                            >  SRR4302542.6406421/1‑94 (MQ=60)
                                                                   GCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGT                           >  SRR4302542.4342712/1‑94 (MQ=60)
                                                                   GCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGT                           >  SRR4302542.6780867/1‑94 (MQ=60)
                                                                   GCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGT                           >  SRR4302542.1954768/1‑94 (MQ=60)
                                                                   GCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGT                           >  SRR4302542.3440871/1‑94 (MQ=60)
                                                                   GCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGT                           >  SRR4302542.4034984/1‑94 (MQ=60)
                                                                   GCCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGT                           >  SRR4302542.1189619/1‑94 (MQ=60)
                                                                   GCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGT                           <  SRR4302542.6454881/94‑1 (MQ=60)
                                                                    CCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTC                          <  SRR4302542.5786004/94‑1 (MQ=60)
                                                                    CCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTC                          <  SRR4302542.1242850/94‑1 (MQ=60)
                                                                    CCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTC                          >  SRR4302542.1504957/1‑94 (MQ=60)
                                                                    CCAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTC                          >  SRR4302542.7433443/1‑94 (MQ=60)
                                                                    CCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTC                          >  SRR4302542.4895467/1‑94 (MQ=60)
                                                                     CAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCC                         <  SRR4302542.3229194/94‑1 (MQ=60)
                                                                     CAACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCC                         <  SRR4302542.2668446/94‑1 (MQ=60)
                                                                     CAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCC                         <  SRR4302542.5090785/94‑1 (MQ=60)
                                                                      AACGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCC                        <  SRR4302542.4927592/94‑1 (MQ=60)
                                                                        CGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCAT                      >  SRR4302542.307622/1‑94 (MQ=60)
                                                                        CGTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCAT                      >  SRR4302542.6455546/1‑94 (MQ=60)
                                                                         GTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATA                     >  SRR4302542.7377044/1‑94 (MQ=60)
                                                                         GTCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATA                     <  SRR4302542.6937749/94‑1 (MQ=60)
                                                                          TCAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC                    <  SRR4302542.4137012/94‑1 (MQ=60)
                                                                          TCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATAC                    <  SRR4302542.6134717/94‑1 (MQ=60)
                                                                           CAAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACC                   >  SRR4302542.3947196/1‑94 (MQ=60)
                                                                            AAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCG                  >  SRR4302542.2692600/1‑94 (MQ=60)
                                                                            AAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCG                  >  SRR4302542.6535845/1‑94 (MQ=60)
                                                                            AAAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCG                  >  SRR4302542.2063448/1‑94 (MQ=60)
                                                                             AAGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGA                 <  SRR4302542.1549180/94‑1 (MQ=60)
                                                                              AGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGAC                >  SRR4302542.3996031/1‑94 (MQ=60)
                                                                              AGACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGAC                >  SRR4302542.1290382/1‑94 (MQ=60)
                                                                               GACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACA               <  SRR4302542.3666955/94‑1 (MQ=60)
                                                                                ACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAA              <  SRR4302542.7559129/94‑1 (MQ=60)
                                                                                ACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAA              >  SRR4302542.3315074/1‑94 (MQ=60)
                                                                                ACATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAA              >  SRR4302542.700667/1‑94 (MQ=60)
                                                                                  ATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAA            >  SRR4302542.4105688/1‑94 (MQ=60)
                                                                                  ATGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAA            >  SRR4302542.1373452/1‑94 (MQ=60)
                                                                                   TGCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAAC           <  SRR4302542.3902260/94‑1 (MQ=60)
                                                                                    GCAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACC          <  SRR4302542.5097972/94‑1 (MQ=60)
                                                                                     CAGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCA         >  SRR4302542.379768/1‑94 (MQ=60)
                                                                                      AGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAA        >  SRR4302542.114405/1‑94 (MQ=60)
                                                                                      AGGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAA        >  SRR4302542.5948215/1‑94 (MQ=60)
                                                                                       GGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAAT       <  SRR4302542.1570026/94‑1 (MQ=60)
                                                                                       GGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAAT       <  SRR4302542.6428333/94‑1 (MQ=60)
                                                                                       GGGCTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAAT       <  SRR4302542.355382/94‑1 (MQ=60)
                                                                                         GCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCC     >  SRR4302542.1378467/1‑94 (MQ=60)
                                                                                          CTGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCC    >  SRR4302542.6463845/1‑94 (MQ=60)
                                                                                           TGGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCA   <  SRR4302542.4904026/94‑1 (MQ=60)
                                                                                            GGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCGGTCCCATACCGACAAAACCAATCCCAA  >  SRR4302542.4083505/1‑94 (MQ=60)
                                                                                            AGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAA  >  SRR4302542.3628641/1‑94 (MQ=60)
                                                                                            |                                                                                             
GAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGGCAGGGCCTGCGCCAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAA  >  NC_000913/4527511‑4527696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑