Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1061	294.1	15065038	98.6%	14854127	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,663	(G)_8→9	noncoding (72/88 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,655	1	.	G	80.5%	390.9 / 114.7	179	noncoding (80/88 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (24/11); new base G (101/43); total (125/54)
Fisher's exact test for biased strand distribution p-value = 8.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGT  >  NC_000913/4603563‑4603739

ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑Ga                                                                                         <  1:4245769/93‑1 (MQ=255)

ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑Ga                                                                                         >  1:7298552/1‑93 (MQ=255)

ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑Ga                                                                                         >  2:7340856/1‑93 (MQ=255)

 tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑g                                                                                     >  1:920709/1‑93 (MQ=255)

 tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑g                                                                                     <  2:2831996/93‑1 (MQ=255)

  aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gg                                                                                    <  2:5058782/93‑1 (MQ=255)

  aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gg                                                                                    >  1:5102239/1‑93 (MQ=255)

   ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggg                                                                                   >  1:1195982/1‑93 (MQ=255)

   ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggg                                                                                   >  1:636872/1‑93 (MQ=255)

    tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggg                                                                                  <  1:5337390/93‑1 (MQ=255)

    tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggg                                                                                  <  1:363356/93‑1 (MQ=255)

    tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggg                                                                                  <  1:6090605/93‑1 (MQ=255)

     gCTGGCATCCTGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggg                                                                                 <  1:1383841/93‑1 (MQ=255)

      cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggggg                                                                                <  1:4569384/93‑1 (MQ=255)

       tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggg                                                                               >  2:6539252/1‑93 (MQ=255)

       tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggg                                                                               >  2:5621770/1‑93 (MQ=255)

         gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggg                                                                             <  1:6801583/93‑2 (MQ=255)

         gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggg                                                                             >  2:1859437/1‑92 (MQ=255)

         gCATAATGCTAATTATAATAAATTTAAATCAGGGAAAAAAAAAAAAAGAAGAAAAAAAAAAGGAAGAAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggg                                                                             <  1:2779295/93‑2 (MQ=255)

           atcatGGTGATGATAATTAATTTGAAAGAGAGATAAAAAATCAAGGAAAAAACAAGAAAGAAAATAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggggggggga                                                                           <  2:5729301/93‑4 (MQ=255)

           atcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACt                                                                           >  2:6266112/1‑93 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggggac                                                                          >  2:5496140/1‑89 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggggac                                                                          >  2:15436/1‑89 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑ggggggggggac                                                                          >  1:999841/1‑89 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑gggggggggact                                                                          >  2:60668/1‑89 (MQ=255)

                gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAAc                                                                      >  1:3816066/1‑93 (MQ=255)

                  tgatgaTAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAAccc                                                                    >  2:5238398/1‑93 (MQ=255)

                   gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAAcccc                                                                   >  2:2560793/1‑93 (MQ=255)

                    atgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAAccc                                                                    >  2:1977572/1‑93 (MQ=255)

                    atgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAAccc                                                                    <  2:7221573/93‑1 (MQ=255)

                      gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAAccccc                                                                  >  1:3919518/1‑93 (MQ=255)

                      gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCa                                                                 >  1:5921780/1‑93 (MQ=255)

                       atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCa                                                                 <  2:3399646/93‑1 (MQ=255)

                       atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCa                                                                 <  1:1073071/93‑1 (MQ=255)

                       aaaaTGAATTTGAATCAGGGATGAAAAATCAAGGAAAAAACAAGAAAGGAAGTAAAGATAATT‑‑GGGGCGGGG‑‑‑GGGGGGGGACTTGAACCCCCa                                                                 <  1:1096042/91‑1 (MQ=255)

                        tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCAc                                                                <  2:3223461/93‑1 (MQ=255)

                        tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGt                                                              >  1:1746595/1‑93 (MQ=255)

                        gaatgaatgTGAATAGGGGAAGAATAAGAAATGAAAAAACAAGAAAAGAAGGAAAAAAAAGT‑‑GGTGC‑‑GAGG‑GGGGGGGGTCTTGAACCCCCAc                                                                <  1:3482379/92‑1 (MQ=255)

                         aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACg                                                               >  2:7518050/1‑93 (MQ=39)

                          aTGAATTTGAATCAGGGATAAAAAATAAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGt                                                              <  2:7021360/93‑1 (MQ=38)

                              aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCg                                                           >  1:1516356/1‑93 (MQ=38)

                              aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGTCTTGAACCCCCACATCCGt                                                          >  2:2712305/1‑93 (MQ=255)

                                  gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGac                                                    <  1:4173190/93‑1 (MQ=255)

                                   aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                    <  2:1670583/93‑1 (MQ=255)

                                       aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                                                  >  1:1875737/1‑93 (MQ=37)

                                        gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                >  2:7081163/1‑93 (MQ=37)

                                        gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                >  1:2356797/1‑93 (MQ=37)

                                         ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTCGAACCCCCACGTCCGTAAGGACACTaa                                               >  2:4584679/1‑93 (MQ=37)

                                         ggATAAAAAACAAAAGAAGAAACAAGAAAGGAAGGAAAGATAAGG‑‑GGTGA‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                               <  2:4536861/93‑1 (MQ=17)

                                          gATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAac                                              <  2:7476318/93‑1 (MQ=37)

                                          aaaaaaaaaaaaAGGAAGAAAAAAAAAAGGAAGTAAAGATAATG‑‑GGGGG‑‑GAGGGGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTaa                                               <  2:1871109/89‑1 (MQ=12)

                                           ataaaaaaTAAAGGAAGAAAAAAAAAAGGAAGAAAAGAAAATTGGGGGGG‑‑GG‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca                                             <  1:5888013/90‑1 (MQ=12)

                                             gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                           >  2:6880182/1‑93 (MQ=25)

                                             aaaaaaaCAAGGAAGAAACAAGAAAGGAAGAAAAAAAAATT‑‑GGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                           <  1:662186/92‑1 (MQ=18)

                                                aaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGaa                                       <  2:4895380/93‑1 (MQ=255)

                                                 aaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                      >  1:839181/1‑93 (MQ=255)

                                                  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCGCCACGTCCGTAAGGACACTAACACCTGAAg                                      >  1:2950030/1‑93 (MQ=21)

                                                   tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                   >  2:5810699/1‑93 (MQ=255)

                                                   tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                   >  2:6837435/1‑93 (MQ=255)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                   >  2:119587/1‑93 (MQ=21)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                   >  1:6739157/1‑93 (MQ=21)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTAAACCCCCACGTCCGTAAGGACCCTAACCCCTGAAGCTa                                   >  1:4259514/1‑93 (MQ=21)

                                                      aGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                  >  1:5751096/1‑93 (MQ=21)

                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                 <  1:4659405/93‑1 (MQ=21)

                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                 >  1:3051779/1‑93 (MQ=21)

                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                               <  2:5902020/93‑1 (MQ=255)

                                                        gaagaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGAAACTAACACCTGAAGCTAgcg                                >  1:2500975/1‑93 (MQ=21)

                                                        gaagaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                >  1:681610/1‑93 (MQ=21)

                                                         aagaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                               >  1:5651577/1‑93 (MQ=18)

                                                         aagaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                               >  1:4866788/1‑93 (MQ=18)

                                                          agaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                              >  1:2148261/1‑93 (MQ=18)

                                                           gaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                             >  1:5362105/1‑93 (MQ=18)

                                                           gaaACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                            >  1:3533818/1‑93 (MQ=255)

                                                            aaaCAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                            <  2:2204511/93‑1 (MQ=18)

                                                              aCAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGGGTCTa                          >  1:440680/1‑93 (MQ=18)

                                                               cAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                          >  2:2785979/1‑93 (MQ=17)

                                                                 aGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                       >  1:212201/1‑93 (MQ=17)

                                                                  gAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                      >  1:6167119/1‑93 (MQ=17)

                                                                  gAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                    >  1:1993203/1‑93 (MQ=255)

                                                                  gAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                    >  2:4995339/1‑93 (MQ=255)

                                                                    aaGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                    >  1:7517842/1‑93 (MQ=17)

                                                                    aaGGAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                    >  1:3137153/1‑93 (MQ=17)

                                                                    aaGGAAGTAAAGATAATT‑‑GGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                   >  2:3495870/1‑93 (MQ=255)

                                                                     aGGAAGAAAAGATAATT‑‑GGGGC‑‑GAGG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                   <  2:1373798/93‑1 (MQ=14)

                                                                       gAAGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                 >  1:4068975/1‑93 (MQ=14)

                                                                        aaGTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                >  2:290453/1‑93 (MQ=14)

                                                                        aaGTAAAGATAATT‑‑GGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                <  2:4350298/93‑1 (MQ=14)

                                                                         aGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc             >  1:3594608/1‑93 (MQ=255)

                                                                          gTAAAGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa              >  1:4805600/1‑93 (MQ=14)

                                                                          gTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc            >  2:3393670/1‑93 (MQ=255)

                                                                            aaaGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc            >  1:5454612/1‑93 (MQ=14)

                                                                             aaGATAATT‑‑GGTGG‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt           <  2:6196243/93‑1 (MQ=11)

                                                                             aaGATAATT‑‑GGTGG‑‑GA‑‑‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc         <  2:3735532/93‑1 (MQ=255)

                                                                             aaGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc         >  2:4440453/1‑93 (MQ=38)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  2:5937678/1‑93 (MQ=11)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  2:5689429/1‑93 (MQ=11)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  1:3405080/1‑93 (MQ=11)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  1:3214846/1‑93 (MQ=11)

                                                                              aGATAATT‑‑GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt          >  2:3965645/1‑93 (MQ=11)

                                                                               gATAATT‑‑GGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc         <  1:6996666/93‑1 (MQ=11)

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATT‑‑GGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGT  >  NC_000913/4603563‑4603739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTT  >  NC_000913/4603571‑4603751

GGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGG                                                                                         >  SRR4302542.5679881/1‑94 (MQ=60)

 GCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGga                                                                                        <  SRR4302542.6868457/94‑3 (MQ=60)

 GCATAATGCTAATTATAATAAATTTAAATCAGGGAAAAAAAAAAAAAGAAGAAAAAAAAAAGGAAGAAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGgg                                                                                        <  SRR4302542.2806061/94‑3 (MQ=16)

   ATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGggac                                                                                      >  SRR4302542.1008445/1‑90 (MQ=60)

    TCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTG                                                                                     >  SRR4302542.7033934/1‑94 (MQ=60)

      ATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTG                                                                                     <  SRR4302542.6235572/94‑1 (MQ=60)

      ATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGggcttta                                                                                   >  SRR4302542.7015935/1‑87 (MQ=60)

       TGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAAC                                                                                  >  SRR4302542.3853810/1‑94 (MQ=60)

        GCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAA                                                                                   >  SRR4302542.2672477/1‑94 (MQ=60)

          TGATGATAATGAATTTGAATAAGGGATGAAAAATCAAGGAAAAAAAAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACC                                                                                 <  SRR4302542.4229735/94‑1 (MQ=60)

            ATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCC                                                                               >  SRR4302542.3119742/1‑94 (MQ=60)

            ATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCA                                                                             >  SRR4302542.5393290/1‑94 (MQ=60)

             TGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCC                                                                              >  SRR4302542.3958196/1‑94 (MQ=60)

             TGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCA                                                                             >  SRR4302542.5979158/1‑94 (MQ=60)

               ATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCAC                                                                            <  SRR4302542.1082292/94‑1 (MQ=60)

               ATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGT                                                                          >  SRR4302542.1761749/1‑94 (MQ=60)

               ATAATGAATTTGAATCAGGGAAGAAAAATCAAGGAAAAAAAAAGAAAGGAAGTAAAGATAATTGGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCAC                                                                            <  SRR4302542.1925449/94‑1 (MQ=60)

               AAAATGAATTTGAATCAGGGATGAAAAATCAAGGAAAAAACAAGAAAGGAAGTAAAGATAATTGGGGCGGGG‑‑‑GGGGGGGGACTTGAACCCCCAC                                                                            <  SRR4302542.1105431/94‑1 (MQ=60)

                AAATGAATTTGAATCAGGGATGAAAAATAAAGGAAAAAAAAAGAAAGGAAGAAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACG                                                                           <  SRR4302542.369163/94‑1 (MQ=60)

                     AATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCG                                                                       >  SRR4302542.1529455/1‑94 (MQ=60)

                     AATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGT                                                                      <  SRR4302542.911615/94‑1 (MQ=60)

                        TTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAG                                                                   >  SRR4302542.4856075/1‑94 (MQ=60)

                          GAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACA                                                               <  SRR4302542.4214348/94‑1 (MQ=60)

                           AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGAC                                                                >  SRR4302542.3632415/1‑94 (MQ=60)

                           AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGTGGGGACTTGAATCCCCACGTCCGTAGGGACAC                                                              >  SRR4302542.3242749/1‑94 (MQ=60)

                            ATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGAC                                                                >  SRR4302542.2061124/1‑94 (MQ=60)

                             TCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC                                                              <  SRR4302542.1141233/94‑1 (MQ=60)

                             TCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGggctttaaacccccccgcccgtagggaacc                                                            >  SRR4302542.4027725/1‑64 (MQ=60)

                              CAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC                                                              >  SRR4302542.1891971/1‑94 (MQ=60)

                               AGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTA                                                            >  SRR4302542.2377055/1‑94 (MQ=60)

                               AGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTA                                                            >  SRR4302542.3435133/1‑94 (MQ=60)

                               AGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTA                                                            >  SRR4302542.1389716/1‑94 (MQ=60)

                                 gaaaaaaaaaaaaagaaaaaaaaaaaAAAGGAAGAAAAAATAATTGGGGC‑‑GAGG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAAC                                                          <  SRR4302542.4136264/68‑1 (MQ=1)

                                 GGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAAC                                                          <  SRR4302542.1003021/94‑1 (MQ=60)

                                   ATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAC                                                        >  SRR4302542.2156868/1‑94 (MQ=60)

                                    gaaaaaaaaaaaaagaaaaaaaaaaaaaagaaaaaaaaaaaattggggggggggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAAC                                                          <  SRR4302542.234708/40‑1 (MQ=0)

                                    TGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCT                                                      <  SRR4302542.5019851/94‑1 (MQ=60)

                                     GAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCT                                                      >  SRR4302542.5974573/1‑94 (MQ=60)

                                     GAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGgggtttgaacccccccggccgtaagggaatcgacacct                                                    >  SRR4302542.3853266/1‑56 (MQ=60)

                                     AAAAAAACAAGGAAGAAACAAGAAAGGAAGAAAAAAAAATTGGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCT                                                      <  SRR4302542.667893/94‑1 (MQ=27)

                                        AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAG                                                  >  SRR4302542.846435/1‑94 (MQ=60)

                                        AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAG                                                  >  SRR4302542.929601/1‑94 (MQ=60)

                                        AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGC                                                 >  SRR4302542.5467148/1‑94 (MQ=60)

                                         AATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCGCCACGTCCGTAAGGACACTAACACCTGAAG                                                  >  SRR4302542.2978270/1‑94 (MQ=60)

                                         AATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAG                                                  >  SRR4302542.2212493/1‑94 (MQ=60)

                                         AATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGC                                                 >  SRR4302542.5812354/1‑94 (MQ=60)

                                           TAAAGGAAGAAAAAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG                                              <  SRR4302542.3044140/94‑1 (MQ=60)

                                            CAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA                                               >  SRR4302542.6805488/1‑94 (MQ=60)

                                            CAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTAAACCCCCACGTCCGTAAGGACCCTAACCCCTGAAGCTA                                               >  SRR4302542.4301455/1‑94 (MQ=60)

                                            CAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG                                              >  SRR4302542.3607572/1‑94 (MQ=60)

                                             AAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG                                              >  SRR4302542.6282522/1‑94 (MQ=60)

                                             AAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG                                              >  SRR4302542.4838953/1‑94 (MQ=60)

                                             AAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG                                              >  SRR4302542.5806924/1‑94 (MQ=60)

                                              AGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                             >  SRR4302542.6771276/1‑94 (MQ=60)

                                              AGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                             >  SRR4302542.3080903/1‑94 (MQ=60)

                                               GGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGAAACTAACACCTGAAGCTAGCG                                            >  SRR4302542.2525223/1‑94 (MQ=60)

                                               GGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG                                            <  SRR4302542.4704956/94‑1 (MQ=60)

                                               GGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG                                            >  SRR4302542.687492/1‑94 (MQ=60)

                                                GAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGC                                           >  SRR4302542.4914168/1‑94 (MQ=60)

                                                GAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGC                                           >  SRR4302542.5706449/1‑94 (MQ=60)

                                                GAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGT                                         <  SRR4302542.683451/94‑1 (MQ=60)

                                                GAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGT                                         >  SRR4302542.1098772/1‑94 (MQ=60)

                                                 ttttaattttttatttaaatttttaaaggtcgtggggcgagGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                             <  SRR4302542.6409569/53‑1 (MQ=0)

                                                 AAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCG                                          >  SRR4302542.686791/1‑94 (MQ=60)

                                                 AAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCG                                          >  SRR4302542.2166781/1‑94 (MQ=60)

                                                  AGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGT                                         >  SRR4302542.5414216/1‑94 (MQ=60)

                                                  AGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAG‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTC                                        >  SRR4302542.3566847/1‑94 (MQ=60)

                                                   aaaaaaaaaaaaaagaaagaaaagaaattggggggggggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGC                                           <  SRR4302542.831675/55‑1 (MQ=0)

                                                   GAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACCCCTGAAGCTAGCGCCTC                                        >  SRR4302542.2963960/1‑94 (MQ=54)

                                                   GAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTC                                        >  SRR4302542.6976426/1‑94 (MQ=60)

                                                    AAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAC                                     >  SRR4302542.4434455/1‑94 (MQ=60)

                                                     AACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGGGTCTA                                      >  SRR4302542.444498/1‑94 (MQ=53)

                                                     AACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTA                                      <  SRR4302542.4688342/94‑1 (MQ=55)

                                                      ACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAC                                     >  SRR4302542.145876/1‑94 (MQ=51)

                                                        tttttatttcattttttaaaagtggtggtgggggGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTA                                      <  SRR4302542.6402955/60‑1 (MQ=0)

                                                        AAGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCA                                   >  SRR4302542.214025/1‑94 (MQ=48)

                                                        AAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                 >  SRR4302542.834474/1‑94 (MQ=52)

                                                         AGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAA                                  >  SRR4302542.6226873/1‑94 (MQ=44)

                                                         AGAAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAA                                  >  SRR4302542.5254898/1‑94 (MQ=46)

                                                         AGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                >  SRR4302542.2010403/1‑94 (MQ=50)

                                                           AAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                >  SRR4302542.7543759/1‑94 (MQ=41)

                                                           AAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                >  SRR4302542.3166972/1‑94 (MQ=41)

                                                           AAAGGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                >  SRR4302542.7591776/1‑94 (MQ=41)

                                                            aaaaaggaagaaaagaaaatgggtggggggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAA                                  <  SRR4302542.7151564/64‑1 (MQ=0)

                                                             AGGAAGTAAAGATAATTGGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTC                               >  SRR4302542.4290405/1‑94 (MQ=36)

                                                             AGGAAGAAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                              <  SRR4302542.1757925/94‑1 (MQ=23)

                                                              GGAAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                             >  SRR4302542.4109101/1‑94 (MQ=32)

                                                                AAGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCC                           >  SRR4302542.2411302/1‑94 (MQ=29)

                                                                AAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                         >  SRR4302542.3628131/1‑94 (MQ=30)

                                                                 AGTAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCA                          >  SRR4302542.4852417/1‑94 (MQ=26)

                                                                   agaaaaaaaaattggggggggggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCC                           <  SRR4302542.3132413/71‑1 (MQ=0)

                                                                   TAAAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACC                        >  SRR4302542.5507602/1‑94 (MQ=22)

                                                                     AAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                      >  SRR4302542.3437001/1‑94 (MQ=14)

                                                                     AAGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                      >  SRR4302542.3245273/1‑94 (MQ=14)

                                                                      AGATAATTGGTGC‑‑GAGG‑GGGGGGGGGCTTGAACCCCCACCTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                     >  SRR4302542.3566721/1‑94 (MQ=8)

                                                                      AGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                     >  SRR4302542.5734390/1‑94 (MQ=9)

                                                                      AGATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                     >  SRR4302542.236956/1‑94 (MQ=9)

                                                                       GATAATTGGGGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                    <  SRR4302542.7065402/94‑1 (MQ=0)

                                                                        ATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                   >  SRR4302542.386825/1‑94 (MQ=5)

                                                                        ATAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGGCCGTAAGGACACTAAAACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                   >  SRR4302542.6024306/1‑94 (MQ=4)

                                                                         taaagtgggggggcgggGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                     <  SRR4302542.6620055/77‑1 (MQ=0)

                                                                         TAATTGGTGC‑‑GAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                   >  SRR4302542.7211694/1‑94 (MQ=2)

                                                                         TAATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCA                  >  SRR4302542.3610849/1‑94 (MQ=2)

                                                                         TAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACA                >  SRR4302542.2692456/1‑94 (MQ=8)

                                                                           ATTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCA                >  SRR4302542.4222153/1‑94 (MQ=0)

                                                                            TTGGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG               <  SRR4302542.6212157/94‑1 (MQ=2)

                                                                              GGTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTC             <  SRR4302542.5015753/94‑1 (MQ=7)

                                                                               tgggtgtggggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG               <  SRR4302542.653427/83‑1 (MQ=3)

                                                                               tggggggggggGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG               <  SRR4302542.2994216/83‑1 (MQ=3)

                                                                               aatggggggggGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG               <  SRR4302542.47877/83‑1 (MQ=3)

                                                                               GTGC‑‑GAGG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA            >  SRR4302542.1654459/1‑94 (MQ=10)

                                                                                 gggggggggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTC             <  SRR4302542.6977701/85‑1 (MQ=9)

                                                                                  ggggggggGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCA            <  SRR4302542.1540428/86‑1 (MQ=11)

                                                                                   gtgggagGGGGGGGGACTTGAACCCCCACGTCCGGAAGGGCACTAACCCCTGAAGCTAGCGCGTTTTCCAATTCCGCCCCCTTCaggtgttaag           <  SRR4302542.6259428/87‑11 (MQ=0)

                                                                                    ggcaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC          <  SRR4302542.3748062/88‑1 (MQ=22)

                                                                                     cgaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCT         >  SRR4302542.2821980/6‑94 (MQ=24)

                                                                                      ggggGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTT        <  SRR4302542.356592/90‑1 (MQ=26)

                                                                                      G‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACT     >  SRR4302542.4640291/1‑94 (MQ=34)

                                                                                       gggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA       <  SRR4302542.1555174/91‑1 (MQ=29)

                                                                                          GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTT    <  SRR4302542.499127/94‑1 (MQ=37)

                                                                                          GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTT    >  SRR4302542.4963452/1‑94 (MQ=37)

                                                                                          GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTT    >  SRR4302542.6902872/1‑94 (MQ=37)

                                                                                           GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTT   >  SRR4302542.2233859/1‑94 (MQ=39)

                                                                                           GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTT   <  SRR4302542.6059222/94‑1 (MQ=39)

                                                                                            GGGGGGCCTTCACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTT  <  SRR4302542.2505883/94‑1 (MQ=38)

GGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTT  >  NC_000913/4603571‑4603751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`