Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 888,260 A→G D261D (GAT→GAC)  b0847 ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913888,2600AG99.7% 979.1 / ‑5.4 284D261D (GAT→GAC) b0847putative transport protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (145/138);  total (146/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 295 total aligned reads displayed.

TTCTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATT  >  NC_000913/888170‑888349
                                                                                          |                                                                                         
ttcttTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACACTATTTCATCGCCCATTTGTAGCACGGCGTc                                                                                         >  2:4862368/1‑93 (MQ=255)
 tcttTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTcc                                                                                        >  1:1649973/1‑92 (MQ=255)
 tcttTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCa                                                                                        <  2:4144120/93‑1 (MQ=255)
  cttTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCAc                                                                                       >  1:4996016/1‑93 (MQ=255)
   tttACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCAcc                                                                                      >  1:3952382/1‑93 (MQ=255)
   tttACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCAcc                                                                                      <  2:4422769/93‑1 (MQ=255)
      tccGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTc                                                                                   <  2:7559502/92‑1 (MQ=255)
      tccGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTc                                                                                   <  1:3184530/92‑1 (MQ=255)
      aCCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTc                                                                                   >  1:2669552/1‑93 (MQ=255)
      aCCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTc                                                                                   >  1:8034308/1‑93 (MQ=255)
       ccGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCt                                                                                  >  2:5247679/1‑93 (MQ=255)
            aCGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGCtt                                                                             >  2:4283777/1‑93 (MQ=255)
            aCGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGAtt                                                                             <  1:6222213/93‑1 (MQ=255)
            aCGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGAtt                                                                             <  1:6561981/93‑1 (MQ=255)
            aCGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGAtt                                                                             >  2:4716511/1‑93 (MQ=255)
             cGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGAttt                                                                            >  1:1173417/1‑93 (MQ=255)
             cGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGAttt                                                                            <  2:1658811/93‑1 (MQ=255)
              ggAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTg                                                                           >  1:5898797/1‑93 (MQ=255)
              ggAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTg                                                                           <  1:3741775/93‑1 (MQ=255)
                aaGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGcc                                                                         <  2:4888436/93‑1 (MQ=255)
                 aGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCa                                                                        <  1:6093262/93‑1 (MQ=255)
                 aGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCa                                                                        <  1:3533982/93‑1 (MQ=255)
                    tGGGATCTAGTAGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCAGTGGTAGCACGGCGTCACCGTCTGGATTTGCCAGaa                                                                     <  1:390164/93‑1 (MQ=255)
                     gggATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAAt                                                                    >  2:3348673/1‑93 (MQ=255)
                     gggATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAAt                                                                    >  2:8797901/1‑93 (MQ=255)
                         tCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCg                                                                <  1:824940/93‑1 (MQ=255)
                            aGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTa                                                             <  1:7767541/93‑1 (MQ=255)
                              tCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACg                                                           <  2:3783642/93‑1 (MQ=255)
                               cGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGt                                                          >  2:6138736/1‑93 (MQ=255)
                               cGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGt                                                          <  1:1438296/93‑1 (MQ=255)
                                  gCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGa                                                       >  2:8284106/1‑93 (MQ=255)
                                  gCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGa                                                       <  2:1991859/93‑1 (MQ=255)
                                    aTGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAAt                                                     >  1:343804/1‑93 (MQ=255)
                                    aTGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAAt                                                     <  2:4562452/93‑1 (MQ=255)
                                     tGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGACTa                                                    <  2:6682174/93‑1 (MQ=255)
                                     tGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATa                                                    <  2:7286870/93‑1 (MQ=255)
                                     tGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGGCTGGATTTGCCAGAATCCCGTTACGCTGAATa                                                    >  2:7381156/1‑93 (MQ=255)
                                         cGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGtt                                                <  2:462726/93‑1 (MQ=255)
                                          gTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTc                                               <  1:4844708/93‑1 (MQ=255)
                                           tCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCCCCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCa                                              <  2:2416927/93‑1 (MQ=255)
                                           tCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCa                                              >  1:2749246/1‑93 (MQ=255)
                                           tCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCa                                              >  1:8451673/1‑93 (MQ=255)
                                           tCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCa                                              <  1:7611126/93‑1 (MQ=255)
                                            cGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCaa                                             <  2:7778468/93‑1 (MQ=255)
                                            cGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCaa                                             <  1:6629057/93‑1 (MQ=255)
                                             gggATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAAt                                            <  2:7723486/93‑1 (MQ=255)
                                              ggATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATg                                           >  1:5779218/1‑93 (MQ=255)
                                               gATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATGCCGTTACGTCGAATACGTTCaagga                                          >  1:7290251/1‑90 (MQ=255)
                                               gATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGt                                          <  2:3063128/93‑1 (MQ=255)
                                                 tAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTag                                        >  1:2926447/1‑93 (MQ=255)
                                                 tAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTag                                        >  1:974305/1‑93 (MQ=255)
                                                 tAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTag                                        <  1:1684289/93‑1 (MQ=255)
                                                  aGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagc                                       <  1:675715/93‑1 (MQ=255)
                                                  aGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagc                                       >  2:3861898/1‑93 (MQ=255)
                                                  aGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagc                                       <  1:5075552/93‑1 (MQ=255)
                                                   tccTGCCAACGCGATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGACTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagca                                      <  2:7393269/92‑1 (MQ=255)
                                                   gCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagca                                      >  2:5072382/1‑93 (MQ=255)
                                                   gCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagca                                      <  1:235399/93‑1 (MQ=255)
                                                    ccTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagcag                                     >  1:1022679/1‑93 (MQ=255)
                                                    ccTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagcag                                     >  1:7955346/1‑93 (MQ=255)
                                                    ccTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagcag                                     >  1:5648062/1‑93 (MQ=255)
                                                     cTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagcagc                                    >  1:402097/1‑93 (MQ=255)
                                                     cTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagcagc                                    >  1:8147342/1‑93 (MQ=255)
                                                     cTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTagcagc                                    <  2:4101421/93‑1 (MQ=255)
                                                      tACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGcc                                   >  2:6342943/1‑93 (MQ=255)
                                                      tACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGcc                                   >  2:1878053/1‑93 (MQ=255)
                                                      tACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGcc                                   >  2:5030725/1‑93 (MQ=255)
                                                      tACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGcc                                   >  2:1285660/1‑93 (MQ=255)
                                                       aCCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCg                                  >  2:5742378/1‑93 (MQ=255)
                                                        cgaaCGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCATTACGTCGAATACGTTCAATGTAGCAGCCgg                                 <  2:3267839/91‑1 (MQ=255)
                                                         cAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGt                                <  2:577261/93‑1 (MQ=255)
                                                          aaCGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGtt                               <  2:2807519/93‑1 (MQ=255)
                                                          aaCGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGtt                               <  1:2099316/93‑1 (MQ=255)
                                                           aCGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGttt                              <  1:1095902/93‑1 (MQ=255)
                                                           aCGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGttt                              <  2:2913873/93‑1 (MQ=255)
                                                           aCGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGttt                              >  1:6403578/1‑93 (MQ=255)
                                                           aCGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGttt                              <  1:3376913/93‑1 (MQ=255)
                                                              cTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTc                           <  2:5489949/93‑1 (MQ=255)
                                                              cTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTc                           >  2:6399356/1‑93 (MQ=255)
                                                               tATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCg                          >  1:519920/1‑93 (MQ=255)
                                                                aTTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGa                         <  1:82838/93‑1 (MQ=255)
                                                                 tttCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGAt                        <  2:586141/93‑1 (MQ=255)
                                                                 tttCATCGCCCATGTGTAGCACGGCGTCTCCGTCTGGATTTGCCAGAATCCCGTTACGTAGAATACGTTCAATGTAGCAGCCGGTTTGTCGAt                        <  1:4485001/93‑1 (MQ=255)
                                                                   tCATCGCCCATTTGGAGCGAGGCGTTACCGTCTGGGTTTGACAGAATCCCCTTACGCCGAATACGTTCAATGTGGCAGCCGGCTTGTCGACaa                      >  2:7184563/1‑93 (MQ=255)
                                                                    cATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATaaa                     >  1:526045/1‑93 (MQ=255)
                                                                    cATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATaaa                     <  2:1194098/93‑1 (MQ=255)
                                                                     aTCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAAt                    >  2:4227382/1‑93 (MQ=255)
                                                                      tCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATa                   >  2:2205863/1‑93 (MQ=255)
                                                                       cGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATAc                  <  1:4332355/93‑1 (MQ=255)
                                                                        gCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATAcc                 >  1:6937810/1‑93 (MQ=255)
                                                                           cATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAg              >  1:3058800/1‑93 (MQ=255)
                                                                           cATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAg              >  2:951852/1‑93 (MQ=255)
                                                                           cATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAg              >  1:741236/1‑93 (MQ=255)
                                                                             tttGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGtt            >  2:2923847/1‑93 (MQ=255)
                                                                              ttGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTc           >  2:6994008/1‑93 (MQ=255)
                                                                                gTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGCTTGTCGTTAAATACCTAGTTCAc         >  2:6904083/1‑93 (MQ=255)
                                                                                   gCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCa      <  1:459002/93‑1 (MQ=255)
                                                                                     aCGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGa    <  1:215820/93‑1 (MQ=255)
                                                                                     aCGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGa    <  2:3684721/93‑1 (MQ=255)
                                                                                       ggCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGAtt  <  1:7452659/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTCTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATT  >  NC_000913/888170‑888349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTG  >  NC_000913/888169‑888353
                                                                                           |                                                                                             
CTTCTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTC                                                                                             <  SRR4302541.6503912/94‑1 (MQ=60)
 TTCTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCA                                                                                            >  SRR4302541.5094357/1‑94 (MQ=60)
 TTCTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCA                                                                                            >  SRR4302541.3990287/1‑94 (MQ=60)
  TCTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCAC                                                                                           >  SRR4302541.5047331/1‑94 (MQ=60)
   CTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACC                                                                                          >  SRR4302541.3991679/1‑94 (MQ=60)
   CTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACC                                                                                          >  SRR4302541.8242184/1‑94 (MQ=60)
   CTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACC                                                                                          >  SRR4302541.2163588/1‑94 (MQ=60)
      TACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTC                                                                                       <  SRR4302541.1900919/94‑1 (MQ=60)
      TACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTC                                                                                       >  SRR4302541.2696083/1‑94 (MQ=60)
      TACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTC                                                                                       >  SRR4302541.8122632/1‑94 (MQ=60)
       TCCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCT                                                                                      <  SRR4302541.3216696/94‑1 (MQ=60)
          GTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGA                                                                                   <  SRR4302541.5804062/94‑1 (MQ=60)
             ACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGCTTT                                                                                >  SRR4302541.3835440/1‑94 (MQ=60)
             ACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTT                                                                                <  SRR4302541.6285790/94‑1 (MQ=60)
             ACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTT                                                                                <  SRR4302541.4774703/94‑1 (MQ=60)
             ACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTT                                                                                >  SRR4302541.1184739/1‑94 (MQ=60)
             ACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTT                                                                                <  SRR4302541.6629083/94‑1 (MQ=60)
              CGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTG                                                                               >  SRR4302541.4408538/1‑94 (MQ=60)
              CGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTG                                                                               >  SRR4302541.6303193/1‑94 (MQ=60)
              CGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTG                                                                               >  SRR4302541.5958829/1‑94 (MQ=60)
               GGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGC                                                                              <  SRR4302541.3779434/94‑1 (MQ=60)
               GGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGC                                                                              >  SRR4302541.6940006/1‑94 (MQ=60)
               GGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGC                                                                              <  SRR4302541.8345225/94‑1 (MQ=60)
                GAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCC                                                                             <  SRR4302541.3296083/94‑1 (MQ=60)
                 AAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCA                                                                            <  SRR4302541.4579737/94‑1 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           <  SRR4302541.3569602/94‑1 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           <  SRR4302541.2969313/94‑1 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           <  SRR4302541.3569603/94‑1 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           <  SRR4302541.3620054/94‑1 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           >  SRR4302541.6173603/1‑94 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           <  SRR4302541.6155458/94‑1 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           <  SRR4302541.5121538/94‑1 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           <  SRR4302541.6155457/94‑1 (MQ=60)
                  AGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAG                                                                           >  SRR4302541.2141189/1‑94 (MQ=60)
                    CTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAA                                                                         <  SRR4302541.3311359/94‑1 (MQ=60)
                     TGGGATCTAGTAGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCAGTGGTAGCACGGCGTCACCGTCTGGATTTGCCAGAAT                                                                        <  SRR4302541.393768/94‑1 (MQ=60)
                     TGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAGT                                                                        >  SRR4302541.6914246/1‑94 (MQ=60)
                     TGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAAT                                                                        >  SRR4302541.7703796/1‑94 (MQ=60)
                     TGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAAT                                                                        <  SRR4302541.393767/94‑1 (MQ=60)
                     TGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAAT                                                                        <  SRR4302541.8139056/94‑1 (MQ=60)
                       GGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCC                                                                      >  SRR4302541.8728132/1‑94 (MQ=60)
                         ATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCG                                                                    <  SRR4302541.3140388/94‑1 (MQ=60)
                         ATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCG                                                                    <  SRR4302541.4037905/94‑1 (MQ=60)
                          TCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGT                                                                   <  SRR4302541.832450/94‑1 (MQ=60)
                           CGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTT                                                                  <  SRR4302541.4013680/94‑1 (MQ=60)
                           CGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTT                                                                  <  SRR4302541.931666/94‑1 (MQ=60)
                           CGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTT                                                                  >  SRR4302541.7032696/1‑94 (MQ=60)
                            GAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTA                                                                 <  SRR4302541.2703865/94‑1 (MQ=60)
                            GAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTA                                                                 >  SRR4302541.1560585/1‑94 (MQ=60)
                             AGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTAC                                                                <  SRR4302541.8531336/94‑1 (MQ=60)
                             AGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTAC                                                                <  SRR4302541.5265077/94‑1 (MQ=60)
                             AGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTAC                                                                >  SRR4302541.349863/1‑94 (MQ=60)
                             AGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTAC                                                                <  SRR4302541.2284488/94‑1 (MQ=60)
                             AGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTAC                                                                <  SRR4302541.2310093/94‑1 (MQ=60)
                             AGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTAC                                                                <  SRR4302541.7850167/94‑1 (MQ=60)
                              GTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACG                                                               >  SRR4302541.8280706/1‑94 (MQ=60)
                              GTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACG                                                               <  SRR4302541.6630558/94‑1 (MQ=60)
                                CGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTC                                                             <  SRR4302541.1452294/94‑1 (MQ=60)
                                CGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTC                                                             <  SRR4302541.7760525/94‑1 (MQ=60)
                                CGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTC                                                             >  SRR4302541.2704085/1‑94 (MQ=60)
                                  GGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGA                                                           >  SRR4302541.6698724/1‑94 (MQ=60)
                                    CATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAAT                                                         >  SRR4302541.346894/1‑94 (MQ=60)
                                    CATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAAT                                                         >  SRR4302541.76217/1‑94 (MQ=60)
                                     ATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATA                                                        >  SRR4302541.6591235/1‑94 (MQ=60)
                                     ATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATA                                                        >  SRR4302541.1058583/1‑94 (MQ=60)
                                     ATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATA                                                        >  SRR4302541.4621/1‑94 (MQ=60)
                                     ATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATA                                                        >  SRR4302541.4429559/1‑94 (MQ=60)
                                      TGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATAC                                                       <  SRR4302541.2944188/94‑1 (MQ=60)
                                      TGGGCGTCCGCATAGCCTACCAACGCTATTTCACCGCCCATTTGTAGCACGGCGCCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATAC                                                       <  SRR4302541.1140908/94‑1 (MQ=60)
                                        GGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGT                                                     >  SRR4302541.1652644/1‑94 (MQ=60)
                                        GGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGT                                                     <  SRR4302541.7539150/94‑1 (MQ=60)
                                           GTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCA                                                  <  SRR4302541.526882/94‑1 (MQ=60)
                                           GTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCA                                                  >  SRR4302541.8542896/1‑94 (MQ=60)
                                           GTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCA                                                  >  SRR4302541.2776865/1‑94 (MQ=60)
                                           GTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCA                                                  <  SRR4302541.4894089/94‑1 (MQ=60)
                                           GTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCA                                                  >  SRR4302541.3560861/1‑94 (MQ=60)
                                            TCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAA                                                 <  SRR4302541.7692454/94‑1 (MQ=60)
                                             CGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAAT                                                >  SRR4302541.8121108/1‑94 (MQ=60)
                                             CGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAAT                                                <  SRR4302541.6697021/94‑1 (MQ=60)
                                              GGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATG                                               >  SRR4302541.3117483/1‑94 (MQ=60)
                                              GGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATG                                               >  SRR4302541.5837980/1‑94 (MQ=60)
                                               GGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATGCCGTTACGTCGAATACGTTCAAgga                                              >  SRR4302541.7368065/1‑91 (MQ=60)
                                               GGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGT                                              >  SRR4302541.7368063/1‑94 (MQ=60)
                                                GATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTA                                             <  SRR4302541.1671239/94‑1 (MQ=60)
                                                 ATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAG                                            >  SRR4302541.620934/1‑94 (MQ=60)
                                                 ATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAG                                            >  SRR4302541.983444/1‑94 (MQ=60)
                                                 ATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAG                                            >  SRR4302541.2955871/1‑94 (MQ=60)
                                                  TAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGC                                           <  SRR4302541.1700842/94‑1 (MQ=60)
                                                  TAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGC                                           >  SRR4302541.2589791/1‑94 (MQ=60)
                                                   AGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCA                                          <  SRR4302541.682094/94‑1 (MQ=60)
                                                   AGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCA                                          <  SRR4302541.5127708/94‑1 (MQ=60)
                                                    GCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTTCGTCGAATACGTTCAATGTAGCAG                                         >  SRR4302541.6514748/1‑94 (MQ=60)
                                                    GCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAG                                         <  SRR4302541.237246/94‑1 (MQ=60)
                                                    GCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAG                                         >  SRR4302541.8043011/1‑94 (MQ=60)
                                                    GCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAG                                         >  SRR4302541.8043010/1‑94 (MQ=60)
                                                    GCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAG                                         >  SRR4302541.5705777/1‑94 (MQ=60)
                                                    GCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAG                                         >  SRR4302541.1032128/1‑94 (MQ=60)
                                                    ACCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAG                                         <  SRR4302541.7518031/94‑1 (MQ=60)
                                                     CCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGC                                        >  SRR4302541.8236433/1‑94 (MQ=60)
                                                     CCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGC                                        >  SRR4302541.57592/1‑94 (MQ=60)
                                                     CCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGC                                        >  SRR4302541.3472381/1‑94 (MQ=60)
                                                     CCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGC                                        >  SRR4302541.405820/1‑94 (MQ=60)
                                                     CCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGC                                        >  SRR4302541.6675007/1‑94 (MQ=60)
                                                      CTACCCACACTCTTTCATCCCCCATTTGTAGCACCGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCC                                       <  SRR4302541.3435682/94‑1 (MQ=60)
                                                      CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCC                                       >  SRR4302541.4085962/1‑94 (MQ=60)
                                                      CTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCC                                       <  SRR4302541.4937710/94‑1 (MQ=60)
                                                       TACCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCG                                      <  SRR4302541.8392738/94‑1 (MQ=60)
                                                         CCAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGT                                    <  SRR4302541.6365498/94‑1 (MQ=60)
                                                          CAACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTT                                   >  SRR4302541.2891272/1‑94 (MQ=60)
                                                           AACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTT                                  <  SRR4302541.2119659/94‑1 (MQ=60)
                                                           AACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTT                                  >  SRR4302541.6468912/1‑94 (MQ=60)
                                                            ACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTG                                 <  SRR4302541.3410578/94‑1 (MQ=60)
                                                            ACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTG                                 <  SRR4302541.1106145/94‑1 (MQ=60)
                                                            ACGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTG                                 <  SRR4302541.2594611/94‑1 (MQ=60)
                                                             CGCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGT                                >  SRR4302541.4819765/1‑94 (MQ=60)
                                                              GCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTC                               >  SRR4302541.8170628/1‑94 (MQ=60)
                                                              GCTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATAAGTTCAATGTAGCAGCCGGTTTGTC                               >  SRR4302541.6820686/1‑94 (MQ=60)
                                                               CTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCG                              >  SRR4302541.747414/1‑94 (MQ=60)
                                                               CTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCG                              <  SRR4302541.1695831/94‑1 (MQ=60)
                                                               CTATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCG                              >  SRR4302541.524778/1‑94 (MQ=60)
                                                                TATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGA                             >  SRR4302541.5161979/1‑94 (MQ=60)
                                                                 ATTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGAT                            <  SRR4302541.83553/94‑1 (MQ=60)
                                                                  TTTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATA                           <  SRR4302541.1797761/94‑1 (MQ=60)
                                                                  TTTCATCGCCCATGTGTAGCACGGCGTCTCCGTCTGGATTTGCCAGAATCCCGTTACGTAGAATACGTTCAATGTAGCAGCCGGTTTGTCGATA                           <  SRR4302541.4530209/94‑1 (MQ=60)
                                                                   TTCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAA                          >  SRR4302541.3964318/1‑94 (MQ=60)
                                                                    TCATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAA                         >  SRR4302541.530969/1‑94 (MQ=60)
                                                                     CATCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAAT                        <  SRR4302541.1368912/94‑1 (MQ=60)
                                                                       TCGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATAC                      <  SRR4302541.5128834/94‑1 (MQ=60)
                                                                        CGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACC                     <  SRR4302541.4375608/94‑1 (MQ=60)
                                                                        CGCCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACC                     >  SRR4302541.7011823/1‑94 (MQ=60)
                                                                          CCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCA                   <  SRR4302541.8360085/94‑1 (MQ=60)
                                                                          CCCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCA                   >  SRR4302541.1330868/1‑94 (MQ=60)
                                                                           CCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAG                  >  SRR4302541.748274/1‑94 (MQ=60)
                                                                           CCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAG                  >  SRR4302541.1171476/1‑94 (MQ=60)
                                                                           CCATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAG                  >  SRR4302541.3089785/1‑94 (MQ=60)
                                                                             ATTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTT                >  SRR4302541.4919742/1‑94 (MQ=60)
                                                                              TTTGTAGCACGGCGTCCCCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACACAGTTC               >  SRR4302541.8012986/1‑94 (MQ=60)
                                                                              TTTGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTC               >  SRR4302541.7438365/1‑94 (MQ=60)
                                                                                TGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCAC             <  SRR4302541.3013440/94‑1 (MQ=60)
                                                                                TGTAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCAC             <  SRR4302541.5649745/94‑1 (MQ=60)
                                                                                  TAGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGC           >  SRR4302541.5403333/1‑94 (MQ=60)
                                                                                   AGCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCA          >  SRR4302541.6147935/1‑94 (MQ=60)
                                                                                    GCACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAG         <  SRR4302541.463376/94‑1 (MQ=60)
                                                                                     CACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGA        >  SRR4302541.5882170/1‑94 (MQ=60)
                                                                                     CACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGA        >  SRR4302541.591373/1‑94 (MQ=60)
                                                                                      ACGGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGAT       <  SRR4302541.217480/94‑1 (MQ=60)
                                                                                        GGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTT     <  SRR4302541.7532255/94‑1 (MQ=60)
                                                                                        GGCGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTT     >  SRR4302541.4976285/1‑94 (MQ=60)
                                                                                          CGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTT   <  SRR4302541.2056948/94‑1 (MQ=60)
                                                                                          CGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTT   <  SRR4302541.505476/94‑1 (MQ=60)
                                                                                          CGTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTT   <  SRR4302541.8807113/94‑1 (MQ=60)
                                                                                           GTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTG  <  SRR4302541.4657957/94‑1 (MQ=60)
                                                                                           GTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTG  <  SRR4302541.4657958/94‑1 (MQ=60)
                                                                                           GTCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTC  >  SRR4302541.6826378/1‑94 (MQ=60)
                                                                                           |                                                                                             
CTTCTTTACCGTTACGGAAGCTGGGATCGAGTCGGGCATGGGCGTCGGGATAGCCTACCAACGCTATTTCATCGCCCATTTGTAGCACGGCATCACCGTCTGGATTTGCCAGAATCCCGTTACGTCGAATACGTTCAATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTG  >  NC_000913/888169‑888353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑