Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,090,676 A→G R279R (CGT→CGC)  ycdS ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,090,6760AG99.3% 952.1 / ‑3.3 277R279R (CGT→CGC) ycdSputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (139/136);  total (139/138)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 288 total aligned reads displayed.

TGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATACGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATA  >  NC_000913/1090587‑1090765
                                                                                         |                                                                                         
tGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATgcgc                                                                                        <  1:4166562/93‑1 (MQ=255)
 tccccGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCt                                                                                       <  1:3738939/92‑1 (MQ=255)
 gCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCt                                                                                       >  1:4011904/1‑93 (MQ=255)
 gCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCt                                                                                       >  2:7734038/1‑93 (MQ=255)
   cccGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTgg                                                                                     >  1:4707113/1‑93 (MQ=255)
   cccGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTgg                                                                                     >  2:5903205/1‑93 (MQ=255)
    ccGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGt                                                                                    >  2:1364901/1‑93 (MQ=255)
     cGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCACCCTGAATGCGCTGGTa                                                                                   >  2:4358026/1‑93 (MQ=255)
     cGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTa                                                                                   >  2:8074093/1‑93 (MQ=255)
     cGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTa                                                                                   <  1:7911477/93‑1 (MQ=255)
     cGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTa                                                                                   <  2:6751575/93‑1 (MQ=255)
      gTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTAc                                                                                  >  1:1766501/1‑93 (MQ=255)
      gTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTAc                                                                                  >  2:7827336/1‑93 (MQ=255)
      gTCTTTTTTAATAGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTAc                                                                                  >  1:1377981/1‑93 (MQ=255)
         ttttttAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTgg                                                                               >  2:6352690/1‑93 (MQ=255)
         ttttttAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTgg                                                                               <  1:616177/93‑1 (MQ=255)
          tttttAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTggg                                                                              <  1:7705941/93‑1 (MQ=255)
           ttttAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGc                                                                             >  2:4548530/1‑93 (MQ=255)
             ttAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAg                                                                           >  1:6482445/1‑93 (MQ=255)
               aaTCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTg                                                                         >  2:4045230/1‑93 (MQ=255)
               aaTCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTg                                                                         >  2:6945494/1‑93 (MQ=255)
                aTCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGc                                                                        <  2:8436624/93‑1 (MQ=255)
                 tCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCg                                                                       <  1:6635160/93‑1 (MQ=255)
                  cGCTAATAGTTAGAAATATCGTCTTTATAACGATCGCTAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCgg                                                                      <  2:6568528/93‑1 (MQ=255)
                   gCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGt                                                                     >  2:4313780/1‑93 (MQ=255)
                    cTGATAGTGAGAAATCACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTc                                                                    <  1:7332095/93‑1 (MQ=255)
                    cTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTc                                                                    <  2:279439/93‑1 (MQ=255)
                     tGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCACCCTGAATGCGCTGGTACTGGGCAGTGCGGTCt                                                                   >  1:961825/1‑93 (MQ=255)
                     tGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCt                                                                   <  2:248853/93‑1 (MQ=255)
                     tGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCt                                                                   >  2:5003389/1‑93 (MQ=255)
                     tGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCt                                                                   >  2:1680681/1‑93 (MQ=255)
                      gATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTg                                                                  >  2:7432483/1‑93 (MQ=255)
                      gATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTg                                                                  >  2:7376833/1‑93 (MQ=255)
                      gATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTg                                                                  >  1:7046709/1‑93 (MQ=255)
                       aTAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTgg                                                                 >  1:8421398/1‑93 (MQ=255)
                         aGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGt                                                               <  2:2173102/93‑1 (MQ=255)
                          gTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGtt                                                              >  1:5485787/1‑93 (MQ=255)
                          gTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGtt                                                              <  2:7567098/93‑1 (MQ=255)
                          gTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGtt                                                              <  1:3316952/93‑1 (MQ=255)
                           tGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTa                                                             <  1:2141801/93‑1 (MQ=255)
                             agaAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATc                                                           <  1:1612298/93‑1 (MQ=255)
                              gaAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCg                                                          >  1:3760078/1‑93 (MQ=255)
                                aaTAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTg                                                        >  2:186661/1‑93 (MQ=255)
                                   aaCGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCTGTGCGGTCTGGGTTATCGTGCca                                                     >  2:6270316/1‑93 (MQ=255)
                                    aCGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGTcac                                                    >  1:3659094/1‑93 (MQ=255)
                                      gTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAg                                                  >  1:4897196/1‑93 (MQ=255)
                                      gTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAg                                                  >  1:8619129/1‑93 (MQ=255)
                                       tCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGa                                                 >  1:8215547/1‑93 (MQ=255)
                                          ttATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAAtt                                              >  2:1230243/1‑93 (MQ=255)
                                          ttATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAAtt                                              >  2:6668651/1‑93 (MQ=255)
                                            ataACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTc                                            <  2:3379670/93‑1 (MQ=255)
                                             taACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCt                                           >  1:5990803/1‑93 (MQ=255)
                                             taACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCt                                           >  2:2501157/1‑93 (MQ=255)
                                              aaCGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTa                                          >  2:3683962/1‑93 (MQ=255)
                                              aaCGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTa                                          >  2:2820184/1‑93 (MQ=255)
                                              aaCGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTa                                          <  1:2219622/93‑1 (MQ=255)
                                               aCGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTaa                                         >  1:2705212/1‑93 (MQ=255)
                                                  aTCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGc                                      >  2:2909564/1‑93 (MQ=255)
                                                   tCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCa                                     <  2:4629584/93‑1 (MQ=255)
                                                   tCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCa                                     <  1:5356649/93‑1 (MQ=255)
                                                    cgcgAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAg                                    >  1:2373106/1‑93 (MQ=255)
                                                       gAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGTTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGt                                 <  2:8628158/93‑1 (MQ=255)
                                                       gAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGt                                 >  1:2900668/1‑93 (MQ=255)
                                                        aGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTa                                <  2:3756070/93‑1 (MQ=255)
                                                         gTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTAt                               <  1:716386/93‑1 (MQ=255)
                                                         gTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTCATCGTGCCACAGAATTTCTAATGCAGCGTAt                               >  1:2100470/1‑93 (MQ=255)
                                                          ttAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTAtt                              <  2:2630162/93‑1 (MQ=255)
                                                          ttAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTAtt                              <  1:6926638/93‑1 (MQ=255)
                                                           taataaCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAACTTCTAATGCAGCGTATTg                             >  2:2702781/1‑93 (MQ=255)
                                                            aataaCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTgg                            <  1:4313061/93‑1 (MQ=255)
                                                             ataaCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTggg                           <  2:4169796/93‑1 (MQ=255)
                                                              taaCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGc                          <  2:767633/93‑1 (MQ=255)
                                                              taaCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGc                          >  1:8642796/1‑93 (MQ=255)
                                                              taaCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGc                          <  2:1095641/93‑1 (MQ=255)
                                                              taaCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGc                          <  1:121603/93‑1 (MQ=255)
                                                                aCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGa                        <  1:8738697/93‑1 (MQ=255)
                                                                aCGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGa                        >  2:7540450/1‑93 (MQ=255)
                                                                  gcgcCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAgg                      <  2:871565/93‑1 (MQ=255)
                                                                  gcgcCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAgg                      <  2:5211951/93‑1 (MQ=255)
                                                                   cgcCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAggg                     <  2:1973279/93‑1 (MQ=255)
                                                                    gcCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGgc                    >  1:8042221/1‑93 (MQ=255)
                                                                     ccAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGgcg                   >  2:7065573/1‑93 (MQ=255)
                                                                      cAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGgcgc                  >  1:6310243/1‑93 (MQ=255)
                                                                       aaGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGcgcg                 >  1:1159920/1‑93 (MQ=255)
                                                                       aaGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGcgcg                 >  2:8371134/1‑93 (MQ=255)
                                                                       aaGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGcgcg                 >  2:3826320/1‑93 (MQ=255)
                                                                        aGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGa                >  2:5575532/1‑93 (MQ=255)
                                                                         gatgatCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGAt               <  1:2439285/93‑1 (MQ=255)
                                                                         gatgatCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGAt               >  2:7968053/1‑93 (MQ=255)
                                                                             atCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGc           <  1:1911466/93‑1 (MQ=255)
                                                                             atCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGc           >  1:1504830/1‑93 (MQ=255)
                                                                               cAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCaa         <  1:30087/93‑1 (MQ=255)
                                                                                aaCCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAAt        >  1:1007238/1‑93 (MQ=255)
                                                                                 aCCTGAATGTGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATg       >  2:4709308/1‑93 (MQ=255)
                                                                                 aCCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATg       >  2:1481497/1‑93 (MQ=255)
                                                                                 aCCTGAACGCGCTGGTACTGGGGAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATg       <  1:1891963/93‑1 (MQ=255)
                                                                                  ccTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATgg      <  1:4582434/93‑1 (MQ=255)
                                                                                    tGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCa    >  1:6732393/1‑93 (MQ=255)
                                                                                     gAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCAt   <  1:5680628/93‑1 (MQ=255)
                                                                                      aaTGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATa  <  2:6048188/93‑1 (MQ=255)
                                                                                         |                                                                                         
TGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATACGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATA  >  NC_000913/1090587‑1090765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATACGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATAACGT  >  NC_000913/1090583‑1090769
                                                                                             |                                                                                             
AATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATG                                                                                               <  SRR4302541.4872848/94‑1 (MQ=60)
AATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATG                                                                                               <  SRR4302541.4966470/94‑1 (MQ=60)
 ATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGC                                                                                              <  SRR4302541.2788230/94‑1 (MQ=60)
    TGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCT                                                                                           <  SRR4302541.4207829/94‑1 (MQ=60)
    TGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCT                                                                                           >  SRR4302541.4051585/1‑94 (MQ=60)
    TGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCT                                                                                           <  SRR4302541.6176611/94‑1 (MQ=60)
     TCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTG                                                                                          <  SRR4302541.3776574/94‑1 (MQ=60)
     GCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTG                                                                                          <  SRR4302541.4694202/94‑1 (MQ=60)
     GCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAAACTGAATGCGCTG                                                                                          >  SRR4302541.8824638/1‑94 (MQ=60)
      CCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGG                                                                                         <  SRR4302541.2157300/94‑1 (MQ=60)
      CCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGG                                                                                         >  SRR4302541.4755100/1‑94 (MQ=60)
      CCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGG                                                                                         <  SRR4302541.7355905/94‑1 (MQ=60)
      CCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGG                                                                                         <  SRR4302541.4173581/94‑1 (MQ=60)
       CCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCGAAGATGATCAACCTGAATGCGCTGGA                                                                                        >  SRR4302541.7018749/1‑94 (MQ=60)
       CCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGT                                                                                        >  SRR4302541.1820188/1‑94 (MQ=60)
       CCCGTCTTGTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGT                                                                                        <  SRR4302541.1923418/94‑1 (MQ=60)
         CGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTAC                                                                                      >  SRR4302541.7552526/1‑94 (MQ=60)
         CGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTAC                                                                                      >  SRR4302541.1783682/1‑94 (MQ=60)
         CGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTAC                                                                                      <  SRR4302541.7998526/94‑1 (MQ=60)
         CGTCTTTTTTAATAGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTAC                                                                                      >  SRR4302541.1391528/1‑94 (MQ=60)
          GTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACT                                                                                     >  SRR4302541.8733964/1‑94 (MQ=60)
          GTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACT                                                                                     <  SRR4302541.534468/94‑1 (MQ=60)
           TCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTG                                                                                    >  SRR4302541.1450332/1‑94 (MQ=60)
             TTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGG                                                                                  <  SRR4302541.621952/94‑1 (MQ=60)
              TTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGC                                                                                 <  SRR4302541.7787893/94‑1 (MQ=60)
               TTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCA                                                                                >  SRR4302541.4420939/1‑94 (MQ=60)
                TTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAG                                                                               >  SRR4302541.6548517/1‑94 (MQ=60)
                 TTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGT                                                                              <  SRR4302541.4778485/94‑1 (MQ=60)
                  TAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTG                                                                             <  SRR4302541.1116243/94‑1 (MQ=60)
                   TATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGC                                                                            <  SRR4302541.6737929/94‑1 (MQ=60)
                   AATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGC                                                                            <  SRR4302541.2851388/94‑1 (MQ=60)
                    ATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCG                                                                           >  SRR4302541.2300401/1‑94 (MQ=60)
                    ATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCG                                                                           <  SRR4302541.2431697/94‑1 (MQ=60)
                     TCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGG                                                                          <  SRR4302541.6703185/94‑1 (MQ=60)
                      CGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGC                                                                         >  SRR4302541.1410653/1‑94 (MQ=60)
                        CTGATAGTGAGAAATCACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCT                                                                       <  SRR4302541.7410388/94‑1 (MQ=60)
                        CTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCACCCTGAATGCGCTGGTACTGGGCAGTGCGGTCT                                                                       >  SRR4302541.970887/1‑94 (MQ=60)
                        CTGATAGTGAGAAATAACGTCTTCATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCT                                                                       <  SRR4302541.4237133/94‑1 (MQ=60)
                         TGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTG                                                                      >  SRR4302541.7121645/1‑94 (MQ=60)
                          GATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGG                                                                     <  SRR4302541.2011569/94‑1 (MQ=60)
                          GATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGG                                                                     >  SRR4302541.8512432/1‑94 (MQ=60)
                             AGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTT                                                                  >  SRR4302541.5906695/1‑94 (MQ=60)
                             AGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTT                                                                  >  SRR4302541.2767568/1‑94 (MQ=60)
                             AGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTT                                                                  <  SRR4302541.6740256/94‑1 (MQ=60)
                             AGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTT                                                                  >  SRR4302541.5542018/1‑94 (MQ=60)
                              GTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTA                                                                 <  SRR4302541.3350233/94‑1 (MQ=60)
                               TGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTAT                                                                <  SRR4302541.2162520/94‑1 (MQ=60)
                                 AGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCG                                                              >  SRR4302541.3797860/1‑94 (MQ=60)
                                 AGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCG                                                              <  SRR4302541.1628317/94‑1 (MQ=60)
                                     ATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCC                                                          >  SRR4302541.8434909/1‑94 (MQ=60)
                                      TAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCA                                                         <  SRR4302541.302738/94‑1 (MQ=60)
                                       AACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGTCAC                                                        >  SRR4302541.3696219/1‑94 (MQ=60)
                                       AACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGTCAC                                                        >  SRR4302541.3696225/1‑94 (MQ=60)
                                         CGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAG                                                      >  SRR4302541.4947165/1‑94 (MQ=60)
                                         CGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAG                                                      >  SRR4302541.8711533/1‑94 (MQ=60)
                                          GTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGA                                                     >  SRR4302541.8305173/1‑94 (MQ=60)
                                            CTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAAT                                                   >  SRR4302541.2357597/1‑94 (MQ=60)
                                            CTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAAT                                                   >  SRR4302541.2213406/1‑94 (MQ=60)
                                            CTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAAT                                                   >  SRR4302541.1799856/1‑94 (MQ=60)
                                            CTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAAT                                                   <  SRR4302541.6996041/94‑1 (MQ=60)
                                               TATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATACGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTC                                                <  SRR4302541.8577749/94‑1 (MQ=60)
                                                ATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCT                                               <  SRR4302541.3785981/94‑1 (MQ=60)
                                                ATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCT                                               >  SRR4302541.6052165/1‑94 (MQ=60)
                                                  AACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAA                                             <  SRR4302541.2241142/94‑1 (MQ=60)
                                                  AACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAA                                             >  SRR4302541.2732118/1‑94 (MQ=60)
                                                   ACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAAT                                            <  SRR4302541.815007/94‑1 (MQ=60)
                                                   ACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAAT                                            <  SRR4302541.7066044/94‑1 (MQ=60)
                                                   ACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAAT                                            >  SRR4302541.6502652/1‑94 (MQ=60)
                                                    CGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATG                                           >  SRR4302541.5335679/1‑94 (MQ=60)
                                                    CGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATG                                           >  SRR4302541.2729274/1‑94 (MQ=60)
                                                     GATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGC                                          <  SRR4302541.371512/94‑1 (MQ=60)
                                                     GATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGC                                          >  SRR4302541.1590494/1‑94 (MQ=60)
                                                      ATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCA                                         >  SRR4302541.3007820/1‑94 (MQ=60)
                                                      ATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCA                                         >  SRR4302541.8827845/1‑94 (MQ=60)
                                                      ATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCA                                         <  SRR4302541.1765924/94‑1 (MQ=60)
                                                       TCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAG                                        >  SRR4302541.2396275/1‑94 (MQ=60)
                                                       TCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAG                                        <  SRR4302541.5411679/94‑1 (MQ=60)
                                                        CGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGC                                       >  SRR4302541.1216863/1‑94 (MQ=60)
                                                        CGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGC                                       >  SRR4302541.4293016/1‑94 (MQ=60)
                                                        CGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGC                                       >  SRR4302541.5553908/1‑94 (MQ=60)
                                                        CGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGC                                       >  SRR4302541.3191143/1‑94 (MQ=60)
                                                         GCGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCG                                      <  SRR4302541.118000/94‑1 (MQ=60)
                                                          CGAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGT                                     >  SRR4302541.2929809/1‑94 (MQ=60)
                                                           GAGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTA                                    <  SRR4302541.5475112/94‑1 (MQ=60)
                                                            AGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTAT                                   >  SRR4302541.2614320/1‑94 (MQ=60)
                                                            AGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTAT                                   <  SRR4302541.60592/94‑1 (MQ=60)
                                                            AGTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTCATCGTGCCACAGAATTTCTAATGCAGCGTAT                                   >  SRR4302541.2120828/1‑94 (MQ=60)
                                                             GTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATT                                  >  SRR4302541.5441141/1‑94 (MQ=60)
                                                             GTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATT                                  <  SRR4302541.723147/94‑1 (MQ=60)
                                                             GTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATT                                  >  SRR4302541.4747596/1‑94 (MQ=60)
                                                             GTTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATT                                  <  SRR4302541.723148/94‑1 (MQ=60)
                                                              TTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTG                                 <  SRR4302541.7000550/94‑1 (MQ=60)
                                                              TTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTG                                 >  SRR4302541.8078120/1‑94 (MQ=60)
                                                              TTAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTG                                 <  SRR4302541.3788968/94‑1 (MQ=60)
                                                               TAATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGG                                >  SRR4302541.7595575/1‑94 (MQ=60)
                                                                AATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGG                               <  SRR4302541.4356134/94‑1 (MQ=60)
                                                                AATAACGCACCAAGATGATCAGCCTGAATGCGCTGGTTCTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGG                               <  SRR4302541.8271758/94‑1 (MQ=60)
                                                                 ATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGC                              >  SRR4302541.8735371/1‑94 (MQ=60)
                                                                 ATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGC                              <  SRR4302541.1264841/94‑1 (MQ=60)
                                                                 ATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGC                              <  SRR4302541.7850026/94‑1 (MQ=60)
                                                                 ATAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGC                              >  SRR4302541.8735373/1‑94 (MQ=60)
                                                                  TAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCG                             <  SRR4302541.156018/94‑1 (MQ=60)
                                                                  TAACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCG                             <  SRR4302541.122571/94‑1 (MQ=60)
                                                                    ACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAG                           <  SRR4302541.6402125/94‑1 (MQ=60)
                                                                    ACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAG                           <  SRR4302541.8832237/94‑1 (MQ=60)
                                                                    ACGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAG                           <  SRR4302541.8832236/94‑1 (MQ=60)
                                                                     CGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGG                          >  SRR4302541.2149883/1‑94 (MQ=60)
                                                                     CGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGG                          <  SRR4302541.2165513/94‑1 (MQ=60)
                                                                     CGCGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGG                          >  SRR4302541.787750/1‑94 (MQ=60)
                                                                       CGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGC                        >  SRR4302541.8130656/1‑94 (MQ=60)
                                                                       CGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGC                        <  SRR4302541.5699970/94‑1 (MQ=60)
                                                                       CGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGC                        <  SRR4302541.3385910/94‑1 (MQ=60)
                                                                       CGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGC                        >  SRR4302541.939076/1‑94 (MQ=60)
                                                                       CGCCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGC                        <  SRR4302541.1777052/94‑1 (MQ=60)
                                                                         CCAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGC                      >  SRR4302541.6374754/1‑94 (MQ=60)
                                                                         CCAAGATGATCAACCTGAATACGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGC                      <  SRR4302541.6924580/94‑1 (MQ=60)
                                                                          CAAGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCG                     >  SRR4302541.1171049/1‑94 (MQ=60)
                                                                            AGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGAT                   >  SRR4302541.4168040/1‑94 (MQ=60)
                                                                            AGATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGAT                   <  SRR4302541.1007147/94‑1 (MQ=60)
                                                                             GATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATC                  <  SRR4302541.397325/94‑1 (MQ=60)
                                                                             GATGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATC                  <  SRR4302541.2463466/94‑1 (MQ=60)
                                                                               TGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGG                >  SRR4302541.5831733/1‑94 (MQ=60)
                                                                               TGATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGG                <  SRR4302541.3537000/94‑1 (MQ=60)
                                                                                GATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGC               >  SRR4302541.1519747/1‑94 (MQ=60)
                                                                                 ATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCA              >  SRR4302541.6635351/1‑94 (MQ=60)
                                                                                 ATCAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCA              <  SRR4302541.1929847/94‑1 (MQ=60)
                                                                                   CAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAAT            >  SRR4302541.1016577/1‑94 (MQ=60)
                                                                                   CAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAAT            <  SRR4302541.30329/94‑1 (MQ=60)
                                                                                   CAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAAT            <  SRR4302541.3933372/94‑1 (MQ=60)
                                                                                   CAACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAAT            <  SRR4302541.4594540/94‑1 (MQ=60)
                                                                                    AACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCCGAGTATTGGGCGAGGGCGCGATCGGCAATG           >  SRR4302541.1911073/1‑94 (MQ=60)
                                                                                    AACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATG           >  SRR4302541.8321221/1‑94 (MQ=60)
                                                                                    AACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATG           >  SRR4302541.6233271/1‑94 (MQ=60)
                                                                                     ACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGG          >  SRR4302541.7202168/1‑94 (MQ=60)
                                                                                     ACCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGG          >  SRR4302541.8564634/1‑94 (MQ=60)
                                                                                     ACCTGAACGCGCTGGTACTGGGGAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGG          <  SRR4302541.1910127/94‑1 (MQ=60)
                                                                                      CCTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGC         <  SRR4302541.4628872/94‑1 (MQ=60)
                                                                                       CTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCA        <  SRR4302541.243481/94‑1 (MQ=60)
                                                                                       CTGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCA        >  SRR4302541.6804251/1‑94 (MQ=60)
                                                                                        TGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCAT       <  SRR4302541.7400586/94‑1 (MQ=60)
                                                                                        TGAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCAT       <  SRR4302541.62894/94‑1 (MQ=60)
                                                                                         GAATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATA      <  SRR4302541.5738587/94‑1 (MQ=60)
                                                                                          AATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATAA     >  SRR4302541.1100340/1‑94 (MQ=60)
                                                                                           ATGCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATAAC    <  SRR4302541.3217057/94‑1 (MQ=60)
                                                                                             GCGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATAACGT  >  SRR4302541.596428/1‑94 (MQ=60)
                                                                                             |                                                                                             
AATTTGCCCCGTCTTTTTTAATCGCTGATAGTGAGAAATAACGTCTTTATAACGATCGCGAGTTAATAACGCGCCAAGATGATCAACCTGAATACGCTGGTACTGGGCAGTGCGGTCTGGGTTATCGTGCCACAGAATTTCTAATGCAGCGTATTGGGCGAGGGCGCGATCGGCAATGGCATAACGT  >  NC_000913/1090583‑1090769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑