Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,207,165	(G)_7→8	coding (442/630 nt)	ycfK →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,207,158	1	.	G	98.0%	686.5 / 4.6	202	coding (435/630 nt)	ycfK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (85/113); total (88/114)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 243 total aligned reads displayed.

ACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGC  >  NC_000913/1207066‑1207244

aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAGAGCATCTGCctct                                                                                             >  2:3843404/1‑93 (MQ=255)

aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCctct                                                                                             <  1:5497850/93‑1 (MQ=255)

aCCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCctct                                                                                             >  2:1564358/1‑93 (MQ=255)

 ccGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑g                                                                                         >  1:1707001/1‑93 (MQ=255)

   ggTTTCATAGCTATACCGGCTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGACGCAAAAGCATCTGCCTCT‑‑‑gag                                                                                       >  1:866487/1‑91 (MQ=255)

    gTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑gggg                                                                                      <  2:1071556/93‑1 (MQ=255)

     tttCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCTGCAAAAGCATCTGCCTCT‑‑‑ggggg                                                                                     >  2:6289624/1‑93 (MQ=255)

     tttCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑ggggg                                                                                     <  1:8415770/93‑1 (MQ=255)

     tttCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑ggggg                                                                                     >  1:6054147/1‑93 (MQ=255)

     tttCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑ggggg                                                                                     <  1:1547480/93‑1 (MQ=255)

     tttCATAGCGATACCGGGTATGATTGGTGGTAGTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑ggggg                                                                                     <  1:5136736/93‑1 (MQ=255)

      ttCATAGCTATACCGGTTATGATTGGTGGTATTTAGCAATCAGTAAGCATTCAGTGGGGGTGGAAGGCCGCAAAAGCATCTTCCTCT‑‑‑ggggg‑‑g                                                                                  <  2:1248227/93‑1 (MQ=255)

       tCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑ggggg‑‑gg                                                                                 >  1:1375485/1‑93 (MQ=255)

         aTAGCTATACCGGTGATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑ggggg‑‑ggga                                                                               <  2:8381389/93‑3 (MQ=255)

           aGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑ggggg‑‑gggatg                                                                             <  1:2322352/93‑5 (MQ=255)

           aGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑ggggg‑‑gggatg                                                                             <  2:4395455/93‑5 (MQ=255)

             cTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGa                                                                            >  1:210248/1‑93 (MQ=255)

              tataCCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGaa                                                                           >  2:5522942/1‑93 (MQ=255)

                   cGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACa                                                                      >  2:4308591/1‑93 (MQ=255)

                    ggTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAg                                                                     <  2:4839593/93‑1 (MQ=255)

                     gTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGt                                                                    >  1:7220884/1‑93 (MQ=255)

                      ttATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGtt                                                                   <  1:1009678/93‑1 (MQ=255)

                      gtATGATTGGTTGTATTGAGCAATCAGTAATCATTCAGTGGGGGGGGAATGCCGCAAAAGCATCTGCATCTG‑‑GGGGG‑‑GGATGGAAATACAGtt                                                                   <  2:6792222/92‑1 (MQ=255)

                       tATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTg                                                                  <  2:3301695/93‑1 (MQ=255)

                         tgtttGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCAGCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTa                                                                <  2:8468195/90‑1 (MQ=255)

                         tGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTa                                                                <  1:4303945/93‑1 (MQ=255)

                           aTTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTAtt                                                              <  2:7215233/93‑1 (MQ=255)

                           aTTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTAtt                                                              >  1:2139991/1‑93 (MQ=255)

                            ttGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTc                                                             <  1:1192952/93‑1 (MQ=255)

                             tggtggTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTcc                                                            <  1:4334214/93‑1 (MQ=255)

                              tggggtATTTCGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCTCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTccc                                                           <  2:8271454/90‑1 (MQ=255)

                                tggtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCgg                                                         >  1:6866043/1‑93 (MQ=255)

                                 ggtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGt                                                        <  1:941264/93‑1 (MQ=255)

                                 ggtATTGAGCAATAAGTAATCATTCAGGGGGGGTGGAATGCCGGAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGt                                                        <  1:663689/93‑1 (MQ=255)

                                  gtcttGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGtt                                                       <  2:1316579/90‑1 (MQ=255)

                                  gtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGtt                                                       >  2:7363210/1‑93 (MQ=255)

                                  gtATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGtt                                                       <  2:88589/93‑1 (MQ=255)

                                   tATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGG‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGtt                                                       >  1:812851/1‑93 (MQ=255)

                                   tATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTg                                                      >  2:5080328/1‑93 (MQ=255)

                                   tATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑GGGGGGCGGATGGAAATACAGTTGTATTCCCGGtt                                                       >  2:8524240/1‑93 (MQ=255)

                                   tATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTATG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTg                                                      <  1:7639194/93‑1 (MQ=255)

                                    aTTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGc                                                     >  2:813793/1‑93 (MQ=255)

                                    aTTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGc                                                     >  2:5621955/1‑93 (MQ=255)

                                     ttGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCg                                                    <  2:2246967/93‑1 (MQ=255)

                                         gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGG‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGttt                                                 >  1:5985025/1‑93 (MQ=255)

                                         gCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTta                                                <  2:8614314/93‑1 (MQ=255)

                                           aaTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaat                                              <  2:8567779/93‑1 (MQ=255)

                                           aaTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaat                                              <  1:5439046/93‑1 (MQ=255)

                                            aTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaata                                             >  1:6537420/1‑93 (MQ=255)

                                            aTCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaata                                             <  2:1672354/93‑1 (MQ=255)

                                             tCAGTATTCATGCAGGGGGGGTGGAAGGCCGCAAAAGCATCGGCCTTTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaataa                                            <  2:8438545/93‑1 (MQ=255)

                                             tCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTtaataa                                            >  1:443655/1‑93 (MQ=255)

                                              cAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTaataat                                           <  2:8704569/93‑1 (MQ=255)

                                              cAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTaataat                                           >  1:8595447/1‑93 (MQ=255)

                                              cAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTaataat                                           <  1:4701173/93‑1 (MQ=255)

                                               aGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATg                                          >  1:2753530/1‑93 (MQ=255)

                                                gTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGc                                         <  2:1575378/93‑1 (MQ=255)

                                                 tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                        <  2:3176446/93‑1 (MQ=255)

                                                 tAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGcc                                        <  1:3829539/93‑1 (MQ=255)

                                                  aaTCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCt                                       >  2:8674108/1‑93 (MQ=255)

                                                   aTCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCtg                                      >  1:1916961/1‑93 (MQ=255)

                                                    tCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGG‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCtg                                      <  2:7612312/93‑1 (MQ=255)

                                                    tCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCtgt                                     <  2:5317724/93‑1 (MQ=255)

                                                      aTTCATTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAAGACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTgtgt                                   <  1:6365078/93‑1 (MQ=255)

                                                       ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGtt                                  <  2:346696/93‑1 (MQ=255)

                                                       ttCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGtt                                  <  1:6001370/93‑1 (MQ=255)

                                                           gTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTGG‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTTcc                               >  1:1151034/1‑93 (MQ=255)

                                                           gTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCg                              >  1:6964546/1‑93 (MQ=255)

                                                            tGGGGGTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgt                             <  2:674326/93‑1 (MQ=255)

                                                              ggggTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttg                           >  2:2568259/1‑93 (MQ=255)

                                                              ggggTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttg                           <  2:588495/93‑1 (MQ=255)

                                                                ggTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttgtt                         <  2:4086189/93‑1 (MQ=255)

                                                                ggTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCgttgtt                         <  1:8731264/93‑1 (MQ=255)

                                                                 gTGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGttgttg                        >  2:3813756/1‑93 (MQ=255)

                                                                  tGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGc                       >  1:636230/1‑93 (MQ=255)

                                                                  tGGAATGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGc                       <  2:6049053/93‑1 (MQ=255)

                                                                    gAATGCCGCAAAAGCATCTGCCTCTGGGGGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGc                       >  1:468762/1‑93 (MQ=255)

                                                                     aaTGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                    <  2:3536354/93‑1 (MQ=255)

                                                                     aaTGCCGCAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCaaa                    <  1:3330495/93‑1 (MQ=255)

                                                                        gCCGCAAAAGCATCTGCCTCT‑‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTAt                >  1:1451131/1‑93 (MQ=255)

                                                                            cacaaGCAACTGCCTCTG‑‑GGGGG‑‑GGATGGACATACTGTAGTATTCCCAGTTGCGTTTAATAATGACTGTGTTGCCGTAGATGCAAAATATAAc             <  1:7792777/90‑1 (MQ=255)

                                                                            cAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGAc             <  1:4006714/93‑1 (MQ=255)

                                                                            cAAAAGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGAc             >  2:7670858/1‑93 (MQ=255)

                                                                             aaaaGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACa            >  1:3035698/1‑93 (MQ=255)

                                                                             aaaaGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACa            >  2:2297750/1‑93 (MQ=255)

                                                                             aaaaGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACa            >  2:460710/1‑93 (MQ=255)

                                                                              aaaGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACaa           <  2:298894/93‑1 (MQ=255)

                                                                               aaGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAAt          <  2:1488466/93‑1 (MQ=255)

                                                                                aGCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATg         <  2:5817188/93‑1 (MQ=255)

                                                                                 gCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGt        <  2:3066577/93‑1 (MQ=255)

                                                                                 gCATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGt        >  2:1903559/1‑93 (MQ=255)

                                                                                  cATCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTc       <  2:4532791/93‑1 (MQ=255)

                                                                                   aTCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATATTGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCa      >  1:3403464/1‑93 (MQ=255)

                                                                                    tCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAg     >  2:7412822/1‑93 (MQ=255)

                                                                                    tCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAg     >  1:2440126/1‑93 (MQ=255)

                                                                                    tCTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAg     <  2:2608532/93‑1 (MQ=255)

                                                                                     cTGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATACCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgc    <  2:2439528/93‑1 (MQ=255)

                                                                                      tGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcg   >  1:3284107/1‑93 (MQ=255)

                                                                                      tGCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcg   >  1:5065870/1‑93 (MQ=255)

                                                                                       gCCTCTG‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAgcgc  <  2:8650296/93‑1 (MQ=255)

ACCGGTTTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTCT‑‑‑GGGGG‑‑GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGC  >  NC_000913/1207066‑1207244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAG  >  NC_000913/1207072‑1207258

TTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑‑‑GG‑GGGGG                                                                                               >  SRR4302541.1389011/1‑94 (MQ=60)

  CATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑‑‑GG‑GGGGGga                                                                                             <  SRR4302541.1147531/94‑3 (MQ=60)

  CATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑‑‑GG‑GGGGGga                                                                                             >  SRR4302541.6610199/1‑92 (MQ=60)

    TAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATG                                                                                            <  SRR4302541.4453135/94‑1 (MQ=60)

    TAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATG                                                                                            <  SRR4302541.8880785/94‑1 (MQ=60)

    TAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATG                                                                                            <  SRR4302541.3674626/94‑1 (MQ=60)

     AGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGG                                                                                           <  SRR4302541.2344889/94‑1 (MQ=60)

     AGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGG                                                                                           <  SRR4302541.4336156/94‑1 (MQ=60)

     AGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGG                                                                                           <  SRR4302541.8341665/94‑1 (MQ=60)

      GCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGA                                                                                          <  SRR4302541.5973317/94‑1 (MQ=60)

      GCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGA                                                                                          >  SRR4302541.211852/1‑94 (MQ=60)

      GCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑‑‑GG‑GGGGGggtgga                                                                                         >  SRR4302541.6615539/1‑88 (MQ=60)

       CTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAA                                                                                         >  SRR4302541.3311381/1‑94 (MQ=60)

       CTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAA                                                                                         <  SRR4302541.8061443/94‑1 (MQ=60)

         ATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAAT                                                                                       >  SRR4302541.8594132/1‑94 (MQ=60)

            CCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAGGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACA                                                                                    <  SRR4302541.4539004/94‑1 (MQ=60)

            CCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACA                                                                                    <  SRR4302541.6936340/94‑1 (MQ=60)

            ACGGTGATGATTGGTGGTAGTGGGCGATCAGTAATCGTTCCGTGGGGGTGGAATGCCGCACAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACA                                                                                    <  SRR4302541.4539002/94‑1 (MQ=55)

             CGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAG                                                                                   <  SRR4302541.7365503/94‑1 (MQ=60)

             CGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAG                                                                                   <  SRR4302541.2473060/94‑1 (MQ=60)

              GGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGT                                                                                  >  SRR4302541.7297914/1‑94 (MQ=60)

                TTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTG                                                                                <  SRR4302541.1019027/94‑1 (MQ=60)

                 TATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGT                                                                               <  SRR4302541.4364530/94‑1 (MQ=60)

                 TATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGT                                                                               <  SRR4302541.2266985/94‑1 (MQ=60)

                 TATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGT                                                                               >  SRR4302541.8846249/1‑94 (MQ=60)

                  ATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTA                                                                              >  SRR4302541.1162199/1‑94 (MQ=60)

                   TGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTAT                                                                             <  SRR4302541.4925868/94‑1 (MQ=60)

                   TGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTAT                                                                             >  SRR4302541.3190830/1‑94 (MQ=60)

                   TGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTAT                                                                             <  SRR4302541.4346925/94‑1 (MQ=60)

                    GATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATT                                                                            >  SRR4302541.2160685/1‑94 (MQ=60)

                     ATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTC                                                                           <  SRR4302541.1879539/94‑1 (MQ=60)

                      TTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCC                                                                          <  SRR4302541.1204515/94‑1 (MQ=60)

                       TGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCC                                                                         <  SRR4302541.4377486/94‑1 (MQ=60)

                         GTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGG                                                                       >  SRR4302541.6939391/1‑94 (MQ=60)

                          TGTTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTATATTCCCGGT                                                                      <  SRR4302541.6289376/94‑1 (MQ=60)

                          TGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGT                                                                      >  SRR4302541.1617271/1‑94 (MQ=60)

                           GGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTT                                                                     <  SRR4302541.950233/94‑1 (MQ=60)

                           GGTATTGAGCAATAAGTAATCATTCAGGGGGGGTGGAATGCCGGAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTT                                                                     <  SRR4302541.669941/94‑1 (MQ=60)

                           GGTATCGAGCAATAAGTCATCATTCAGTGGGGGTGGAATGCCGAAAAACCATCTGCCCC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTT                                                                     <  SRR4302541.5312455/94‑1 (MQ=60)

                            caattaaaCAATCAGTAATCATTCAGTGGGCGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTG                                                                    <  SRR4302541.8244198/86‑1 (MQ=60)

                            GTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGG‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTT                                                                     >  SRR4302541.820279/1‑94 (MQ=60)

                             TATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGC                                                                   <  SRR4302541.8013830/94‑1 (MQ=60)

                             TATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTA‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGC                                                                   <  SRR4302541.7720651/94‑1 (MQ=60)

                                  AGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGG‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTT                                                               >  SRR4302541.6046292/1‑94 (MQ=60)

                                     AATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATA                                                           >  SRR4302541.3389116/1‑94 (MQ=60)

                                     AATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATA                                                           <  SRR4302541.5495093/94‑1 (MQ=60)

                                     AATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATA                                                           <  SRR4302541.1458915/94‑1 (MQ=60)

                                     AATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATA                                                           <  SRR4302541.5494857/94‑1 (MQ=60)

                                     AATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATA                                                           >  SRR4302541.6604179/1‑94 (MQ=60)

                                      ATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAA                                                          >  SRR4302541.6429073/1‑94 (MQ=60)

                                      ATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAA                                                          >  SRR4302541.447831/1‑94 (MQ=60)

                                       TCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAAT                                                         >  SRR4302541.8687664/1‑94 (MQ=60)

                                       TCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAAT                                                         <  SRR4302541.2701427/94‑1 (MQ=60)

                                       TCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAAT                                                         <  SRR4302541.7168887/94‑1 (MQ=60)

                                        CAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATG                                                        <  SRR4302541.4749098/94‑1 (MQ=60)

                                        CAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATG                                                        >  SRR4302541.2781196/1‑94 (MQ=60)

                                        CAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATG                                                        <  SRR4302541.4749097/94‑1 (MQ=60)

                                         AGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGC                                                       >  SRR4302541.69981/1‑94 (MQ=60)

                                           TAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCT                                                     <  SRR4302541.3868113/94‑1 (MQ=60)

                                            AATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTG                                                    >  SRR4302541.1935402/1‑94 (MQ=60)

                                            AATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTG                                                    <  SRR4302541.6528208/94‑1 (MQ=60)

                                              TCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTG                                                  >  SRR4302541.6492727/1‑94 (MQ=60)

                                               CATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGT                                                 <  SRR4302541.692432/94‑1 (MQ=60)

                                                ATTCATTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAAGACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTT                                                <  SRR4302541.6430163/94‑1 (MQ=60)

                                                 TTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTG                                               <  SRR4302541.6062849/94‑1 (MQ=60)

                                                 TTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTG                                               <  SRR4302541.6062847/94‑1 (MQ=60)

                                                 TTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTG                                               <  SRR4302541.5971005/94‑1 (MQ=60)

                                                  TCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGC                                              >  SRR4302541.5924819/1‑94 (MQ=60)

                                                   CAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCC                                             >  SRR4302541.6088359/1‑94 (MQ=60)

                                                    AGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TGG‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTTCC                                             >  SRR4302541.1162023/1‑94 (MQ=60)

                                                    AGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCG                                            >  SRR4302541.7038782/1‑94 (MQ=60)

                                                     GTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGT                                           <  SRR4302541.2990817/94‑1 (MQ=60)

                                                      TGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTT                                          <  SRR4302541.1478341/94‑1 (MQ=60)

                                                      TGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTT                                          <  SRR4302541.1478342/94‑1 (MQ=60)

                                                       GGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑‑‑GGAGGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTG                                         <  SRR4302541.3921689/94‑1 (MQ=60)

                                                        GGGGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGT                                        <  SRR4302541.5237254/94‑1 (MQ=60)

                                                          GGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTG                                      <  SRR4302541.8824745/94‑1 (MQ=60)

                                                          GGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTG                                      <  SRR4302541.1234753/94‑1 (MQ=60)

                                                          GGTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTG                                      >  SRR4302541.2150887/1‑94 (MQ=60)

                                                           GTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGC                                     >  SRR4302541.642209/1‑94 (MQ=60)

                                                           GTGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGC                                     <  SRR4302541.7369210/94‑1 (MQ=60)

                                                            TGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCA                                    >  SRR4302541.8633736/1‑94 (MQ=60)

                                                            TGGAATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCA                                    >  SRR4302541.8726958/1‑94 (MQ=60)

                                                             GGAATGCCGCAAAAGCATCTGCCTC‑TGGGGG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGC                                     >  SRR4302541.473223/1‑94 (MQ=60)

                                                               AATGCCGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAAT                                 <  SRR4302541.3363853/94‑1 (MQ=60)

                                                                 TGCCGCAAAAGCATCTGCCTC‑T‑‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTAT                              >  SRR4302541.1465274/1‑94 (MQ=60)

                                                                    CGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGA                            >  SRR4302541.1840391/1‑94 (MQ=60)

                                                                    CGCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCGGTGTTGCCGTTGTTGCAAttattga                            >  SRR4302541.2340280/1‑87 (MQ=60)

                                                                     GCAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGAC                           <  SRR4302541.3328228/94‑1 (MQ=60)

                                                                      CAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACA                          >  SRR4302541.2166677/1‑94 (MQ=60)

                                                                      CAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACA                          <  SRR4302541.4046352/94‑1 (MQ=60)

                                                                      CAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACA                          >  SRR4302541.876898/1‑94 (MQ=60)

                                                                      CAAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACA                          >  SRR4302541.3066352/1‑94 (MQ=60)

                                                                       AAAAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAA                         >  SRR4302541.4194857/1‑94 (MQ=60)

                                                                         AAGCATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATG                       <  SRR4302541.5476584/94‑1 (MQ=60)

                                                                           GCATCTGCCTC‑T‑‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCA                    >  SRR4302541.7782522/1‑94 (MQ=60)

                                                                            CATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATATTGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCA                    >  SRR4302541.3437427/1‑94 (MQ=60)

                                                                            CATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCA                    <  SRR4302541.2354825/94‑1 (MQ=60)

                                                                            CATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCA                    >  SRR4302541.377261/1‑94 (MQ=60)

                                                                            CATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCA                    <  SRR4302541.1303072/94‑1 (MQ=60)

                                                                             ATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAG                   >  SRR4302541.2464318/1‑94 (MQ=60)

                                                                             ATCTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAG                   <  SRR4302541.2100018/94‑1 (MQ=60)

                                                                              TCTGCATCGT‑‑‑GG‑GGGGGAGGGAAATACATTTGGATTCCCGGTTGCGTTTAATAATGCCTGGGTTGCCGTTGTTGCAAATTATGACAATGTCAGC                  <  SRR4302541.7414039/94‑1 (MQ=60)

                                                                               CTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCG                 >  SRR4302541.5117947/1‑94 (MQ=60)

                                                                               CTGCCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCG                 >  SRR4302541.3317198/1‑94 (MQ=60)

                                                                                  CCTC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCAC              >  SRR4302541.1592222/1‑94 (MQ=60)

                                                                                    TC‑TG‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCT            >  SRR4302541.8464837/1‑94 (MQ=60)

                                                                                       ctggGG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCTTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCT            <  SRR4302541.4564399/90‑1 (MQ=60)

                                                                                       G‑‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATC         >  SRR4302541.7301684/1‑94 (MQ=60)

                                                                                           GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCA        >  SRR4302541.4034963/1‑94 (MQ=60)

                                                                                           GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCA        <  SRR4302541.8744369/94‑1 (MQ=60)

                                                                                            G‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAA       >  SRR4302541.5529725/1‑94 (MQ=60)

                                                                                              GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAAT      <  SRR4302541.1759449/94‑1 (MQ=60)

                                                                                                GGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGC    >  SRR4302541.432870/1‑94 (MQ=60)

                                                                                                GGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGC    >  SRR4302541.2894408/1‑94 (MQ=60)

                                                                                                 GGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCA   >  SRR4302541.2830543/1‑94 (MQ=60)

                                                                                                  GATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAG  >  SRR4302541.7159840/1‑94 (MQ=60)

TTCATAGCTATACCGGTTATGATTGGTGGTATTGAGCAATCAGTAATCATTCAGTGGGGGTGGAATGCCGCAAAAGCATCTGCCTC‑T‑‑‑GG‑GGGGGATGGAAATACAGTTGTATTCCCGGTTGCGTTTAATAATGCCTGTGTTGCCGTTGTTGCAAATTATGACAATGTCAGCGCACCTATCAATGCAG  >  NC_000913/1207072‑1207258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`